Composition, bioactive microporous material imitating natural osseous structures, and method of manufacturing

FIELD: medicine.

SUBSTANCE: invention concerns medicine, particularly composition for bioactive microporous material, including medical glass reduced to powder, hydroxyapatite powder and carbonate porophore, with addition of zeolite for micropore structure generation and enhancement of durability of ceramic glass materials and articles.

EFFECT: efficient method of obtaining bioactive ceramic glass materials based on claimed composition and applicable in prosthetics of osseous tissues, teeth or their fragments of complex form or mixed porosity structure.

12 cl, 6 ex, 1 tbl

 

The technical field to which the invention relates.

The invention relates to medicine, specifically to compositions, the bioactive composite materials based on them and the way they are received, and can be used for the manufacture of bone implants in reconstructive surgery.

The level of technology

In the world annually more than 400 TYSABRI replacement of various bones and joints. In Russia, according to data for 2005, the total number of operations is about 60 tysw year, while the share of bone-plastic surgery with the use of artificial implant materials does not exceed 5 tysw year.

Before the healthcare of the Russian Federation the task is already in 2007 goudoti the total number of operations, and in 2008 to bring them up to 180 thousand

This involves the development of advanced medical technologies, including the production of medicines, materials and methods of their application. In this regard, the development of new bone substitute materials, technologies of their manufacture and methods of use is an extremely important task.

Accumulated clinical experience in the use of artificial materials has allowed to define the main desired these requirements:

- implant materials should not cause immune-conflict reactions, i.e. to be biocompatible with the body of the recipient;

- chemical, mines the General composition, the structure and properties they should be close to the mineral matrix of bone tissue;

- the material should not change the chemical nature of the zone installation in the body;

- partially or completely and in a short time to adjust to the bone of the recipient without changes in volume;

- be able to manufacture implants for replacement of defects of complex anatomical forms and heterogeneous in structure, such as the bones of the vault of the skull, spine, eye orbit, the chin Department, the zygomatic and temporal bones.

Currently on the world market implant of the most promising materials for bone replacement are bioactive non-metallic materials because they combine necessary for application in medicine properties: biocompatibility with living tissue of the body and durability.

These materials include biostable, bioceramics, biopolymers, bioglass ceramics and biocomposites.

Special interest porous biocomposite materials (BM) on hydroxyapatite (HA) and tricalcium phosphate (β-TKF) (see, for example, patent application U.S.№20070040478, 20060282166, 20060052479, 20050255159, 20030152606 and others).

They are structural analogues of the mineral matrix of bone, have similar mineral and chemical composition and thus the nutrient, comparable physico-mechanical and bio-energetic properties. These materials also possess unique biochemical properties that make them resorbable in the environment of the organism and the possibility of occurrence in it of the process of the volume of osteogenesis, leading to the formation of living bone tissue at the site of implant placement.

However, current knowledge about the structure of bone and osteogenesis impose additional requirements, which most of biocomposite materials (BM) on hydroxyapatite (HA) and tricalcium phosphate (β-TKF) do not possess.

First of all, these materials must meet certain requirements for porosity, i.e. to have an open porosity level 50-70% with a pore size of 100-500 microns for germination in the material of bone cells and blood vessels. Next, they must be highly permeable to interstitial fluids of the body, i.e. to be open micropores of less than 100 microns to provide education in implant blood clot, which is a prerequisite to the process of osteogenesis.

Thus, the most promising materials must have a bimodal size of the pore structure, the ratio of which is directed and quantitatively handling.

In addition, these materials are characterized by insufficient mechanical Hara is the characteristics, which usually increase due to the increase of the size of the implant or introducing titanium valves.

Thus, the problem of regulating the porosity and increase the strength of this class of implant materials is an open and highly relevant. Moreover, it can be argued that none of the existing osteoplastic materials based on hydroxyapatite (HA) and tricalcium phosphate (β-TKF) is not fully in line with these requirements.

Closest to the proposed invention is a material for bone implants based on medical glass and hydroxyapatite and a method of manufacturing this material (patent RU 2053737 - prototype).

The disadvantage of this known material is its low strength, component 18-20 MPa, and the lack of adjustable bimodal pore structure, which have artificial implants was proved above.

The invention, therefore, is an expansion of the means of this purpose in mind the relevance for the national bone surgery problems of artificial bone implants), obtaining implant materials with controllable bimodal pore structure with high mechanical properties, as well as the development of techno is Oginga of the manufacture method.

Disclosure of inventions

The problem is solved in that in the composition for bioactive microporous material containing powdered medical glass powder of hydroxyapatite and carbonate blowing agent, enter the zeolite, which forms microporous structure and increases the strength of ceramic materials and products, allowing you to use it as their active component, and to obtain bioactive glass-ceramic materials and products suitable for prosthetic bone tissue, teeth or their parts with a complex shape or a mixed pore structure.

In more detail, the invention consists in the fact that the proposed composition comprises a zeolite with a grain size of 1 mm and a pore size of from 0.5 nm to 20 μm, granulated together with hydroxyapatite to granule size from less than 50 μm to 1 mm, in the following ratio, wt.%:

Glass20-80
Hydroxyapatite10-50
Zeolite10-40
The pore-forming0,1-10

Hydroxyapatite is mainly the ratio of CA: P 1-2, and even more preferably of 1.66-1,67.

As the matrix glass is preferable to use a neutral copper is Ty aluminosilicate glass brand NA content (wt.%): 73,0 SiO 2,3,5 Al2About3, 2.5 V2O3, 1,0 MgO, 7,0 CaO, 11,0 Na2O, 2.0 To2O. This glass does not contain in its composition of oxides of heavy metals such as lead, zinc, barium, strontium, antimony, arsenic, and others whose presence is unacceptable from a Toxicological point of view, and has a number of important properties:

- high chemical resistance under the influence of solutions of various substances (II hydrolytic class), including preparations based on blood plasma;

- water extract from this glass has a neutral reaction;

- low tendency to crystallization due to the high degree of connectivity kremnekislotnosti radical.

Based on the proposed composition obtained bioactive microporous material that mimics the natural bone structure.

The material has a porous structure, mainly with a total porosity of 40-80%, open porosity of 30-70%, with pore size of 100 to 600 μm at the level of 30-50% of the total number of pores in the material, for germination in the material of bone cells and blood vessels, the micropores with a size of 20-100 μm at the level of 50-70% of the total number of pores in the material, to provide education in the material of the blood clot, which is a prerequisite to the process of osteogenesis, has a bulk density from 300-2500 kg/m3the compressive strength of 20-50 MPa, withstands compression the way the definition and dynamic loads on 1 cm 2the surface of 0.5 to 5 kN.

In the specified material fastening diploid human cells occurs in macroecono size 100-600 microns, and are present in the material micropores with a size of 20-100 μm accelerate the process of cell division.

The proposed material simulates spongy bone with a total porosity of from 40 to 80% and a pore size from 20 to 600 microns or cortical bone with a total porosity of from 20 to 50% and a pore size of from 20 to 100 μm and is capable of withstanding compression loads on 1 cm2the surface is not less than 2 kN.

The material may be a multilayer material with differentiated by size and then focused on the nature of their distribution structure and, thus, it can mimic the natural bone structure with the transition from dense cortical layer spongy layer.

It is also proposed a method of manufacturing a bioactive microporous material that mimics the natural bone structure in which the above composition is formed and subjected to heat treatment with the separation stages of sintering and foaming.

The heat treatment is carried out for 3 to 10 hours in two stages, first gradually heated to a temperature of 600-750°C, then rapidly to a temperature of 800-1000°and maintain the mixture at this maximum temperature for 5-60 minutes.

Forming blanks m is material to obtain a multi-layered or complex in form of products includes hydration original compositions up to 30 wt.% with the formation of densely Packed frame of granules of hydroxyapatite and zeolite, which ensures that the products are free of cracks and other structural defects.

Patented the application of the proposed bioactive material with controlled pore structure for the manufacture and replacement of multiple or mixed porous structure of the bone fragments, as well as the use of zeolite as an active component of bio-ceramic materials and products on the basis of glasses, providing education in their micropores to stimulate osteogenesis.

It should be noted that the zeolites used in medicine, in particular, it is known the use of zeolites in the composition of the bio-ceramic compositions for fillings in dentistry (patent application U.S. No. 2006088480, Japan patent No. 6001708, the United Kingdom patent No. 2238044; here we use the well-known ion-exchange properties of zeolites as a source of calcium or silver ions, providing the necessary, including antibacterial properties of the sealing material).

Know the use of zeolite in biomaterial for bone prosthesis on the basis of hydroxyapatite and thermoplastic polymer (inert basis of biomaterial) (patent application U.S. No. 20060052479, French patent 2722694), which possess good biocompatibility and mechanical properties.

However, the role of zeolites in achieving these properties in patent publications is not disclosed.

the moreover, in the well-known inventions as the base material using a thermoplastic polymer, its chemical and physico-chemical properties very different from those used in this application fundamentals - inorganic aluminosilicate glass.

First of all, you can indicate such a significant, distinct from the properties of the polymer) property used in the present invention fundamentals as related to its chemical nature to the nature of the zeolite is at a high temperature heat treatment of the latter can engage in chemical and physico-chemical interaction with the glass, resulting in a change might occur (destruction), as in the original structure of the zeolites, and implemented phase transformations used in the glass, in particular, can occur in the processes of crystallization.

Thus, the use of zeolites in the proposed glass-ceramic materials to improve the strength properties of the material and the formation of a bimodal pore structure with the possibility of quantitative changes of the ratios of these two types of pores in the material) is not known from the prior art and clearly follows from the known properties of the zeolites. The set of distinctive features other objects of the present invention is also not disclosed in the prior art that allows AET in General to talk about the novelty and inventive step of the invention.

The implementation of the invention

In the developed material are mainly two types of pores: micropore size of 20-100 μm, obtained at the expense of own microscopic pores of the grains of zeolite and formed in the foaming process of stellamarisa in thermal decomposition of the blowing agent, and macropores from 100 to 600 μm, formed intergranular voids of the filler granules (hydroxyapatite, zeolite).

The pores of the developed material are open, which allows to relate the material to the discharge kolonizirali bioactive materials, the connection of which with the bone tissue is provided by the ingrowth of bone cells into the material by volume of colonization open pores.

Modification of the structure and material properties is achieved by changing particle size distribution, the ratio of components of the initial mixtures and synthesis conditions.

Particle size of the filler (hydroxyapatite, zeolite) varies from >50 μm to 1 mm

The content of the components varies within the following limits:

Amorphous phasefrom 20 to 80
Crystalline phasefrom 20 to 80
Adjustable porosityfrom 20 to 80

As a blowing agent in the synthesis danovaroussinova material is calcium carbonate, which is introduced into the mixture in amounts of from 0.1 to 10.0 wt.%.

The manufacturing process of the material is as follows.

Produce joint granulating powder of hydroxyapatite grains (granules) of zeolite a given size, limiting the contact surface of the zeolite with glass layers which are inert with respect to the glass hydroxyapatite. This procedure is necessary to prevent healing microporous structure of zeolite granules melt glass in the sintering process. Calculated in accordance with the specified part number hydroxyapatite, zeolite, matrix glass and blowing agent are mixed to obtain a homogeneous mixture.

The prepared mix is placed in the form of corundum, titanium or stainless steel and subjected to heat treatment with the separation of the stages of sintering and foaming in the range from 600 to 1000°With (at first slowly heated to a temperature of 600-750°C, then rapidly to a temperature of 800-1000°C, soaking at this temperature 5-60 minutes). The porosity of the finished product depends on the ratio of its components (the matrix filler), their fractional composition and varies from 40 to 80% when the open porosity of from 30 to 70%. The compressive strength of a material depends on its porosity and is in the range from 20 to 50 MPa. The temperature specification of the project for a biocomposite material changes with increasing filler content and the growth of the size of its granules in the initial powder mixtures and varies from 780-800° To 830-850°S, this corresponds to a decrease in the viscosity of stellamarisa 107-105,8PA·C. the dwell Time of the material in an electric furnace at a maximum temperature varies depending on the size of the sample and the specified end structure and varies from 5 to 60 minutes.

In the case of manufacturing a multilayer implant with adjustable texture (porosity, pore size), physical (strength, permeability) and biological (resorbable cell division) by the properties of the layers, it is advisable to moisten the mixture to 30 wt.%, ensure uniform compaction of the mix due to the tightening forces of interfacial tension in the system "filler - matrix-water with the formation of close-Packed framework of granular filler, which reduces material shrinkage during drying and sintering to 1%. It is established that at the prescribed temperature-time mode in multilayer composites retains all the regularities of the formation of pore structures of different types in separate layers.

Example 1 (Composition 1).

Matrix glass, hydroxyapatite, zeolite and a pore-forming, calcium carbonate is taken in relation to 19.9:50:30:0.1 wt.% and thoroughly mixed. And previously conducted joint granulation grains of zeolite size of 200 μm and powder of hydroxyapatite to the size of the of renal 200-900 mm.

Example 2 (Composition 2)

Matrix glass, hydroxyapatite, zeolite and a pore-forming, calcium carbonate is taken in the ratio of 70:10:10:10 wt.% and thoroughly mixed. And previously conducted joint granulation grains of zeolite size of 200 μm and powder of hydroxyapatite to the size of the granules 200-800 microns.

Example 3. The method of obtaining material from the composition of example 1.

Obtained in example 1, the mixture is loaded into platinum, titanium or corundum form, compacted and placed in an electric furnace. Next, produce heating to a temperature of 750°With speeds of no more than 8-10 degrees/min, then raising the temperature to 800°and maintain the mixture at this temperature for 60 minutes. Cooling the resulting material to room temperature is carried out with regard to the temperature range of annealing of the matrix glass.

Example 4. The method of obtaining material of the composition according to example 2.

Obtained according to example 2, the mixture is loaded into platinum, titanium or corundum form, compacted and placed in an electric furnace. Next, produce heating to a temperature of 600°With speeds of no more than 8-10 degrees/min, then raise the temperature to 1000°and maintain the mixture at this temperature for 5 minutes. Cooling the resulting material to room temperature is carried out with regard to temperature is nterval annealing of the matrix glass.

Example 5. The material characteristics of the composition according to example 1 and 2 respectively.

Ready-made materials of the compositions of examples 1 and 2, respectively obtained in examples 3 and 4, is subjected to mechanical processing before the opening of the pore structure.

The material obtained according to example 3 has the following characteristics :

Water absorption: 50%.

The total porosity: 80%.

The open porosity: 70% (87% for the total porosity).

The closed porosity of 10%.

The average pore size: 300-400 microns.

The share of long 100-600 microns: 70%.

The proportion of pores of less than 100 μm: 30%.

Material density: 2.5 g/cm3.

Compressive strength: 40 MPa.

The material obtained according to example 4 has the following characteristics:

Water absorption: 50%.

The total porosity: 50%.

The open porosity: 35% (70% for the total porosity).

The closed porosity: 15%.

The average pore size: 100-200 microns.

The share of long 100-600 μm: 50%.

The proportion of pores of less than 100 μm: 50%.

Material density: 2.0 g/cm3.

Compressive strength: 50 MPa.

The materials are able to withstand a compression load on 1 cm2surfaces 4 and 5 kN, respectively.

Example 6. Biological studies of composite materials. Biological studies on the possibility of a graft cell cultures was held in Ekaterinburg Institute of cellular medicine.

In the operation of the cell culture used medical immunobiological product "cell Cultures of diploid human replacement therapy", which is a strain of diploid human cells LEC 4(81)obtained in Ekaterinburg research Institute of viral infections health Ministry (Glinsky, NP and other AC No. 1147748) and the last control security viruses and bacteria in accordance with ANGUISH 4.1/4.2 588-96, the control of sterility, in accordance with ANGUISH 4.1/4.2 588-96, evaluation of the oncogenic potential and tumorigenicity in accordance with who requirements and JV 3.3.2.561-96.

For the analysis used a diploid cell culture, which is in a stable stage of development in an active growth phase. The passage was carried out at the rate of 100 testlets on 1 cm2sample. Used in the experiment, the concentration of cells was ensured by the formation of a monolayer in control at 3-5 days of growth. The analyzed material was soaked for 24 hours in an environment of maintaining cell cultures, in order to stabilize the chemical composition and normalization of pH. Then Wednesday maintain decanted and transferred material into the culture vial containing a suspension of cells in the growth medium. The growth rate of cells was determined from the change in pH of the growth environment, compared to control cells at neutral glass).

For studies of the prepared samples in the form of plates 25*30*10 mm Porous structure of the material does not allow for morphological analysis directly on the material. To identify possible morphological changes and umali from the culture vial, were rinsed in Hanks solution to remove reprecipitate cells and culture was transferred into a clean vial with a growth medium and a piece of cover glass. Continued cell growth, spreading material on the glass, forming a monolayer, the rate of its formation is an additional criterion for the activity of the cells. Then the glass was removed for morphological analysis. Morphological parameters were determined by known methods (blumkin V.N., Zhdanov V.M., 1973).

Results

The results of the study of the materials developed on the possibility of replanting medical immunobiological preparation "Culture cells diploid human replacement therapy" are summarized in table:

Table

The growth of cell cultures on substrates with different porosities
No. sampleThe open porosity, % (% calculated on the total porosity)The rate of impregnation saline, mm/minThe presence of cell growth
187 (example 3)40Growth active
24620Growth no
370 (example 4)80The growth act is wny
43820Growth weak
55025Growth active
69285Growth active

The results showed that the active growth phase cell cultures of diploid human best occurs at high values of open porosity and permeability, as occurred in samples 1, 3 and 6.

The obtained results allow to conclude that the bimodal pore structure of the material has an extremely important role in the creation of favorable conditions for the preservation and growth of cells. For example, sample 2, which had a predominantly large pores more than 500 μm, did not show a good result, while in the rest of the samples in which there was a sufficiently large number of pore size of 20-100 μm, was active cell growth. This suggests that in addition to cell size of 100-500 microns, which sits bone cell, play an important role micropores of a size less than 100 microns, which create favorable conditions for its preservation, and division.

Thus, biological tests developed single-layer and multilayer porous BM showed the suitability of their application as wear the I cell culture and production on their basis of living implants", positively influencing the degree of osseointegration in the body.

1. Composition for bioactive microporous material that mimics the natural bone structure containing powdered medical glass powder of hydroxyapatite and carbonate blowing agent, characterized in that it further comprises grains of zeolite with a grain size less than 1 mm and a pore size of from 0.5 nm to 20 μm, granulated together with hydroxyapatite to the size of the granules from more than 50 μm to 1 mm, in the following ratio, wt.%:

Glass19,9-80
Hydroxyapatite10-50
Zeolite10-40
The pore-forming0,1-10

2. The composition according to claim 1 characterized in that the quality of medical glass is used neutral medical aluminosilicate glass brand NA content, (wt.%): SiO273,0, Al2About33.5,2About32,5, MgO 1.0, CaO 7,0, Na2O 11,0, K2About a 2.0.

3. The composition according to claim 1, characterized in that it contains hydroxyapatite ratio of CA : P 1-2, mainly of 1.66-1,67.

4. Bioactive microporous material that mimics the natural bone structure, characterized in that it is obtained by heat treatment to the position according to claims 1-3, he has a pore structure characterized by the following parameters: total porosity of 40-80%, open porosity of 30-70%, where the pores with the size of 100-600 μm account for 30-50%of the micropores with a size of 20-100 μm - 50-70% of the total number of pores, respectively; and has a volumetric weight of 300-2500 kg/m3the compressive strength of 20-50 MPa, can withstand a compression load on 1 cm2the surface in 0.5 to 5 kN.

5. The material according to claim 4, characterized in that it additionally contains a diploid human cells, thus fixing the cells in the macropores size 100-600 microns, and are present in the material micropores with a size of 20-100 μm accelerate the process of dividing the established cells.

6. The material according to claim 4, characterized in that it mimics the spongy bone layer with a total porosity of from 40 to 80% and a pore size from 20 to 600 microns.

7. The material according to claim 4, characterized in that it mimics the cortical layer of bone with a total porosity of from 20 to 50% and a pore size of from 20 to 100 μm and can withstand a compression load on 1 cm2the surface is not less than 2 kN.

8. The material according to claim 4, characterized in that it is a multilayer material with differentiated by size and then focused on the nature of their distribution structure.

9. Material of claim 8, characterized in that it mimics the natural bone structure with the transition from plotno the cortical layer spongy layer.

10. A method of manufacturing a bioactive microporous material that mimics the natural bone structure according to claims 4 to 9, characterized in that a composition according to claims 1 to 3 is formed and then thermoablative within 3-10 hours in two stages to separate stages of sintering and foaming: first, it is gradually heated to a temperature of 600-750°C, then rapidly to a temperature of 800-1000°C, and then incubated the mixture at the specified temperature for 5-60 minutes

11. The method according to claim 10, characterized in that the molding blanks additionally includes hydration original compositions up to 30 wt.% with the formation of close-Packed framework of granules of hydroxyapatite and zeolite.

12. Application of bioactive microporous material according to claims 4 to 9, for replacement of bone fragments with a complex shape and/or mixed with the first structure, in which these bone fragments occurs stimulation of osteogenesis.



 

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7 cl, 7 dwg, 1 tbl, 6 ex

FIELD: chemistry.

SUBSTANCE: nano-sized highly-pure hydroxylapatite (HAP) is obtained in form of alcohol colloid solution (gel), which can be used for production of medicinal preventive preparations in stomatology, for applying bioactive coating on bone implants. Method includes hydroxylapatite synthesis by adding solution of orthophosphoric acid to calcium hydroxide solution and exposure to ultrasound impact, and ethyl or isopropyl alcohol is added to colloid of highly-pure nano-sized hydroxylapatite with concentration from 5% to 40±2% in order to obtain concentration of hydrooxylapatite in alcohol from 0.2 to 20%. After that alcohol mixture is processed with ultrasound with frequency 10-50 kHz during 1-2 hours.

EFFECT: alcohol colloid of nano-sized hydroxylapatite with high stability.

2 cl, 3 ex

FIELD: medicine.

SUBSTANCE: described is material for closing osteal defects at reparative-plastic operations, manufacturing osteal implants, replacement of defects at various osteal pathologies. The material is made of calcium phosphate, represents particles of carbon-replaced hydroxyapatite with the general formula: Ca10(PO4)x(OH)y(CO3)z where 5<X<6, 0<Y<2, 0<Z<1, contains of 0.6 to 6.0 wt % CO32- groups, with an adjustable nuclear ratio of calcium/phosphorus of 1.5 to 2.1. The material is made in the form of porous spherical granules with diameter of 100 to 1000 microns, having a rough microrelief of an external surface, with the size of pores from 0.5 to 15.0 mcm at the general open porosity of 50 to 80% and a specific surface of 0.3 to 0.6 m2/g. The material possesses the following properties: high adhesion in relation to cells; combination of osteoconductivity properties and osteoinductance; affinity of chemical and phase structure of implanted material to structure of replaced tissue; adjustability of rate of dissolution at its replacement by an osteal tissue; possibility of three-dimensional uniform filling of the osteal defect repeating its form. The material can be saturated with autologous mesenchymal stem cells.

EFFECT: improved properties of the material.

2 cl, 1 tbl, 6 dwg

FIELD: medicine, in particular agents for bone defect compensation.

SUBSTANCE: in the first embodiment claimed material includes hydroxyapatite with tricalcium phosphate and additionally it contains osteogenesis-inducing composition of non-collagen bone tissue proteins in specific component ratio per 100 g of material. In the second embodiment claimed material includes hydroxyapatite with tricalcium phosphate and/or collagen and additionally it contains osteogenesis-inducing composition of non-collagen bone tissue proteins. In the third embodiment claimed material includes hydroxyapatite with tricalcium phosphate and/or collagen and additionally it contains osteogenesis-inducing composition of non-collagen bone tissue proteins and double distilled water in specific component ratio per 100 g of material.

EFFECT: material with high osteoplastic ability.

9 cl, 3 ex, 6 dwg

FIELD: medicine.

SUBSTANCE: described are implants based on biodegradable thixotropic compound with pseudo-plastic properties and implant injected under skin or into skin in fibrous tissue. Containing microparticles of at least one biocompatible ceramic compound in suspension, in at least one liquid carrier containing at least one compound based hyaluronic acid and at least one biodegradable thixotropic compound with pseudo-plastic properties. Also disclosed is kit for preparation such implants directly before application, as well as implant production and using for filling of crinkles, and/or skin cavity, and/or cicatrices.

EFFECT: implants of simplified injection.

14 cl, 4 ex

FIELD: medicine, orthopedics, oral surgery, surgical stomatology.

SUBSTANCE: the present innovation refers to the system of delivering medicinal preparations and could be applied for filling in osseous defects or as a matrix for cell cultures. Porous hydroxyapatite ceramics with bimodal distribution of pores contains thin intragranular (under 10 mcm diameter) and large-scale interpenetrating intergranular pores, the size being above 100 mcm, at total quantity of about 41-70 rot.%. The method deals with manufacturing spherical granules of about 400-600 mcm diameter that contain hydroxyapatite powder and gelatin, pressing these spherical granules under 10-100 MPa pressure and thermal treatment at about 900-1250°C at keeping from 30 to 300 min. The innovation enables to create ceramics with bimodal distribution of pores.

EFFECT: higher efficiency of manufacturing.

2 cl, 1 tbl

Transplant mixture // 2301684

FIELD: medicine.

SUBSTANCE: transplant mixture has liophylized allogenic bone tissue and allogenic hydroxyapatite and patient autoblood platelets gel with Metronidazole, taken in the following components proportions (%): liophylized allogenic bone tissue - 65; allogenic hydroxyapatite - 10; patient autoblood platelets gel - 20; Metronidazole - 5.

EFFECT: enhanced effectiveness of treatment; no clamps required; reliably and tightly closed bone tissue defect; accelerated regenerate reorganization; improved antiseptic and immunomodulating action.

FIELD: medical-destination materials and products.

SUBSTANCE: preparation of hydroxyapatite-based porous ceramic granules, which can be used to fill bone defects in traumatology, orthopedics, maxillofacial surgery, and surgical stomatology, comprises preliminary synthesis of calcium phosphate powder with Ca/P ratio 1.5-1.67 and preparing suspension with 10% gelatin solution in proportion of 0.5-3 mL solution per 1 g powder at solution temperature 10 to 39° C. Suspension of hydroxyapatite in aqueous gelatin solution is then dispersed in neutral liquid vegetable oil, resulting mixture is stirred by blade stirrer at rotation speed 100 to 1500 rpm. Under effect of surface tension, spherical granules are formed, which are washed, dried, and subjected to heat treatment at 900-1250° C.

EFFECT: enabled preparation of granules with controlled size and open porosity occupying 20 to 80% of the volume.

1 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: mixture has lyophilized allogenic bone tissue and additionally has allogenic hydroxyapatite and patient autoplasma enriched in blood platelets. The components are taken in ratio in %: lyophilized allogenic bone tissue - 60; allogenic hydroxyapatite - 20; patient autoplasma enriched in blood platelets - 20.

EFFECT: provided increase in transplantation mixture plasticity; usability for modeling transplant from the given mixture of required form; tightly closed bone tissue defect.

4 dwg

FIELD: medical technologies; production of the carbonate hydroxyapatite ceramics.

SUBSTANCE: the invention is pertaining to the charge for the carbonate hydroxyapatite ceramics. The charge for production of the ceramic material on the basis of the carbonate of hydroxyapatite may be used for filling-up the osteal defects in traumatology and orthopedics, the maxillofacial surgery and the surgical stomatology. The charge of the carbonate hydroxyapatite additionally contains 2-20 mass % of the potassium carbonate. The technical result of the invention is production of the dense ceramic material and reduction of the temperature of its sintering. At that its kilning may be conducted in the air medium in the ordinary kilns without application of the special cost intensive inventory maintaining the set humidity and the gas medium.

EFFECT: the invention ensures production of the dense ceramic material and reduction of the temperature of its sintering.

1 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: method involves applying hydroxyapatite collagen material plate so that plate size is selected to be by 5-8 mm greater than trepanation opening.

EFFECT: enhanced effectiveness in making plastic repair of maxillary sinus; excluded allergic responses.

FIELD: medical equipment.

SUBSTANCE: the invention refers to biocompatible wearproof nanostructure thin-film materials on the basis of titan carbonitride, used as membranulas for manufacturing imlants, working under load. Total concentration of the basic and additional elements in a covering has the following ratio: where Xi - total concentration of basic elements Ti, Ta, C, N in the covering, Yj - total concentration of additional elements Ca, Zr, Si, K, Mn, O, P in a covering, concentration of elements in the covering being chosen at the following ratio of components, at.wt%: Ti - 30-50; Ta 6-50; C - 15-40; N - 0-35; O - 5-25; Ca - 0-7; Zr - 0-20; Si - 0-30; P - 0-1.5; Mn - 0-1.0; K - 0-1.0.

EFFECT: high hardness of covering; low elasticity module; high durability of coupling with a substrate; low factor of friction and rate of deterioration; high firmness to elastic deformation of destruction and plastic deformation; low roughness of the surface; negative charge of the surface in physiological mediums; bioactive surface; biocompatibility and absence of toxicity.

16 ex, 1 tbl

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