Method of treatment of malignant tumours in dogs

FIELD: medicine; veterinary science.

SUBSTANCE: method involves diagnosis, electric magnetic field radiation of tumour to prepare for removal of malignant tumour, surgical removal of tumour and postoperative follow-up of animal. Herewith in preoperative period, animal is exposed to variable local alternating very-low-frequency pulsed electric magnetic field (AVP EMF) radiation of tumour at magnetic field induction B=(20÷80) mtesla, impulse time ti=0.75 sec, pause time tp=0.5 sec, exposure duration 30-60 minutes within 20-40 days. Surgical operation of malignant tumour removal is carried out 3-7 day AVP EMF exposure course.

EFFECT: high efficiency, low risk of recurrent tumour.

2 cl, 10 tbl, 2 dwg

 

The invention relates to veterinary medicine, more specifically to Oncology, and can be used in veterinary clinics for the treatment of animals with malignant tumors.

It is known the use of ionizing radiation for the treatment of cancer through the remote and intracavitary irradiation of tumors (see Lindenblatt L.D. Medical radiology. - M.: Medicine, 2000, 672 S.).

The damaging effect of radiation therapy can effectively destroy their tissues. However, in the impact zone are healthy tissue. They suffer the same defeat as the tumor. In radiation therapy, there is a limit, when the repeated exposure of the same field not valid (on dose). After irradiation, as a rule, there are General (systemic and local adverse reactions of the organism. In addition, radiotherapy requires expensive equipment and specialized training of staff.

Known use in Oncology non-ionizing electromagnetic radiation or electromagnetic fields (EMFs). Thermal effects of microwave fields are used to protivoopujolevy hyperthermia at temperatures above 43,5°With (see Donchenko ASSUCH-hyperthermia in Oncology // abstracts of the "Medical physics" - 95 - M.: 1995. - P.113). It is possible that p is ocessi denaturation, leading to intoxicate and irreversible damage healthy tissues and the whole organism.

Cited studies of anti-stress and anti-tumor effects of weak ultralow-frequency EMF to slow the growth of tumors in rats (males), carcinogenesis which was initiated by the introduction into the skin of the back benzopyrene. It is established that the EMFs, profilaktorua the onset of oxidative stress and functional integrity of cell membranes of the brain, mediated influence on the dynamics of the development of neoplasms (reduced rate of growth) (see A.S. No. 826587, A61 1/42, Publ. 30.08.90., Bull. No. 32).

Another way of indirect effects on tumors was used in the postoperative period in the treatment generalizovannoe forms of melanoma. Stimulation of the neuroendocrine system was achieved by simultaneous exposure to the whole body of the patient ultralow-frequency periodic EMF with amplitude ˜ 3 MT. For effects were used magnetotherapy apparatus providing rotation of the magnetic field in the working cavity with frequency (50-100 Hz. (see Bakhmut N.G. Treatment generalizovannoe form of melanoma vortex magnetic field. // Russian oncological journal. - 2000,№6. P.32-35). The study's author concludes significantly less postoperative recurrence of the disease when the conditions is EMF exposure compared to control.

Positive dynamics of indirect effects of ultralow-frequency EMF indicates good prospects of its application in Oncology diseases.

Attempts to directly impact Transcaucasian EMFs on tumor tissue began to realize, when was the claimed method of treatment of malignant tumors by exposure to intermittent sinusoidal EMF (the amplitude of the induction was 40 MT.) (see A.S. No. 522688, A61 No. 1/42, Publ. 05.03.77. Bull. 9). According to the results of the studies indicated a softening of the tumor tissue. However, the effectiveness of such effects was low compared to the used radiation and chemotherapy. As a result, the method has not received clinical application.

In the ongoing search for more effective ways of influencing intensive ultralow-frequency EMFs on malignant neoplasms. So, for these purposes was used briefly ˜ 5 min pulsed EMF exposure when the amplitude of the magnetic induction of up to 1.8 T, the intervals between the pulses was 60 MS. The studies were carried out on outbred rats with neoplasms of the soft tissues of the thigh (sarcomas). The effects of magnetotherapy was manifested in necrotic processes of different intensity, which ultimately led to growth inhibition or complete regression of the tumor. This result d is let the search for the optimal parameters and modes of effects of ultralow-frequency EMFs on neoplasms (see Kaplan M.A. prospects of application of high-intensity pulsed magnetic fields in the treatment of malignant tumors. // Russian oncological journal. - 1998, No. 8, p.34-37).

The objective of the invention is to develop a method of treatment of malignant tumors in animals by local effects on tumors intensive ultralow-frequency EMFs. The parameters of the EMF, the method and irradiation regimes should provide selectivity against malignant tumors. The effects of this exposure on tumor tissue comparable to the effects obtained after courses of chemotherapy (conducted according to standard schemes) without hitting the adjacent healthy tissue. This will allow preoperational exposure of the animal in an outpatient setting using the secondary skills that do not require areas with special protection, without unnecessary injury and stress, and high efficiency of treatment and ensure the reduction of risk of relapse in the future, using domestic installations and appliances - emitters ultralow-frequency electromagnetic fields.

The task is solved by the fact that after diagnosis, comprehensive treatment is carried out in the period of pre-irradiation revealed malignant swollen is whether the local alternating ultralow-frequency pulsed electromagnetic field (ZIYA EMF) when worked and reasonable parameters: the amplitude of the magnetic field In=20÷ 80 MT; at the time of an electromagnetic pulse tuti=0,75 s, pause time tbreaks=0.5 seconds, with the duration of the session electromagnetic radiation 30-60 minutes within 20-40 days daily, and surgery to remove irradiated ZIYA EMF malignant tumors spend 3-7 days after completion of radiation therapy. In cases of relapse after surgery, re-irradiation of ultralow-frequency pulsed electromagnetic field of the affected area two courses for 15-20 sessions during the interval between courses of not more than 20 days. When irradiated using two counter-directed along one axis of the coil, emit an electromagnetic field, while the axis of the coils ZIYA EMFs have on the center of the irradiated tumors, and the coils have the top and bottom of the tumor at a distance of 2-5 cm from the animal, when the working area of irradiation ≈10÷30 cm2.

The method is characterized by the fact that in order to suppress the tumor cells are exposed to a bipolar alternating ultralow-frequency pulsed EMFs, with adjustable parameters generated in the space between the two counter-directed along one axis of the solenoids, providing selective regarding healthy morphologically reversible and irreversible changes in tissues of malignant tumors.

Re the implementation of the method is designed and manufactured by experienced magnetotherapeutic installation, one of the main elements of which is the generator ZIYA EMF.

The exposure apparatus is a fixed installation with the emitter ZIYA EMF, which is made in the form of two toroidal solenoids.

The coils are made of non-magnetic material and positioned on the upper and lower bases of the working hours, with a possible location between the object of influence. The design allows you to adjust the distance between the coils, and also to carry out rotation of the coils relative to the rack to change the position of the impact zone.

The power unit operates from AC mains frequency of 50 Hz. The power supply is connected in parallel to the coils of the magnetic field generator is a step-down transformer with a secondary winding to which the voltage is applied to the unit pulse shaping. The formation of the pulses is performed using thyristors. Work unit pulse shaping is carried out through the device run as system control and monitoring from a personal computer (figure 1). The need to include between your computer and install intermediate system management and monitoring using L-card due to the fact that the standard interfaces of the personal computer does not allow to generate special signals that understand the mouth of the STS launch. In memory of L-a map containing a number of functional blocks, loads of special control program that commands from the computer interacts with the installation. The amplitude of the induction EMF, the duration of forward and reverse pulses and the subsequent pauses can be adjusted individually. Simultaneously with the work control program, information about the exposure parameters and the image of the axial cross-section distribution of the magnetic field (the values of amplitudes) is recorded on the computer display. The generated field is assymmetrical. It is mainly concentrated in the working area between the coils.

Temporal structure of the generated magnetic field is determined by the parameters of the electric circuit of the coils, its resistance R and inductance L, which together determine its sluggishness. The change in the magnetic field pulse at increasing his forward and fall back edge is expressed as the ratio In(t)=about·e-t/twhere t=L/R is the relaxation time. The amplitude of pulsations of the magnetic induction ˜ 1 MT with a frequency of 100 Hz pulse allow the resulting excitation of the action potential. This improves the efficiency of impulse action.

The relaxation time of rise and fall times of the pulse edges is ˜50 MS. For distances IU the control coils of the order of 30 cm, the amplitude of the magnetic induction on the axis in the planes of the end faces of the coils ˜ 80 MT. For the given parameters field magnetic induction depends on the distance of honey coils and the magnitude of the current in the windings.

Characteristics used in the study experienced magnetotherapeutic installation are shown in table 1.

Table 1
Characteristics of magnetotherapy installation
FeaturesValue
The amplitude of the magnetic field (In)up to 80 MT
The current in the coil (l)27
The distance between the coils (h)(30-50)cm
The cross-sectional area of the working window (S)˜ 10÷30 cm2
The pulse duration (tAnd)0.01
Pause duration (tP)0.05
Power consumption (W)1.5 kW

In the course of the research was conducted periodic monitoring of work magnetotherapeutic installation. Contactless were measured current in the coils. The magnetic field was controlled using a Hall sensor. The pulse parameters were reproduced on the oscilloscope at given distances between the coils and installed temporary mode is X.

Material for research on the rationale of the proposed method were selected purebred dogs.

Before the experiment was examined about 150 animals (dogs). Of them for the experiment were selected 54 animals. The object of the study were 44 purebred dogs with spontaneous malignant tumors by histological structure - high - and usernotification ductal carcinoma one or more of the mammary glands. Dogs were divided into groups. Animals in the amount of 10 dogs formed the control group. They ZIYA EMFs were not carried out, and was only surgical removal of tumors with histological examination of the material obtained. The time of tumor development (from discovery before treatment) ranged from 8 to 24 months. The tumor size at the beginning of treatment (exposure) ranged from 2.5 to 7 cm in diameter. Age of dog - 6 to 13 years. The weight of animals - from 7 to 25 kg

Animals (depending on the results of histological research) were divided into subgroups:

- Subgroup And spent a number of sessions 20, session duration 30 minutes

Subgroup And is divided by the duty cycle:

- duty cycle, tand=(10-15)seconds; tp=(1-2)s;

- duty cycle, tand,=0,75 s; tp=0.5 sec.

The subgroup held a number of sessions 20, p is abolitionist session 45 min t and=0,75 s; tp=0.5 sec.

The subgroup - povedenie number of sessions 40, session duration 30 min; tAnd=0,75 s; tp=0.5 sec;

The sessions were conducted daily.

In the original version In=(20-80), the duration of tandand tpwas 15 sec and 1 sec, respectively. In the future, taking into account the results of preliminary studies the parameters used ZIYA EMFs were adopted: tand=0.75 in, tn=0,5c,˜40÷80 MT.

In the irradiation process, the animal was placed between the coils on a special table. The axis of the coils corresponded to the center of the irradiated tumors. During procedures, the body temperature of the animals was not different from the original and meets 38-39°C.

All animals before, middle and after a course of exposure by standard methods was determined hematological, biochemical and immunological parameters. In individual animals was also performed control of clinical laboratory tests one month after the end of the course of action.

At the Hematology research (erythrocytes, hemoglobin, leukocytes, eosinophils, neutrophils: young, stab, segmented; lymphocytes, monocytes, ESR) - the counting of blood cells in the samples was carried out in the chamber Goryaeva, leukocyte profile and the amount of hemoglobin definition is ranged according to standard techniques.

Biochemical analysis of blood parameters (total protein, total bilirubin, sugar, urea, creatinine) were performed by using photospectrometry apparatus photoelectric Photometer KLF - 3".

The study of the immune status included quantitative determination of T-(F-ROLL)- and-(M-ROL)lymphocytes in the reaction spontaneous rosethorne with sheep erythrocytes and mouse (number of cells, number of Lymph, AKL, T/a, LTI, T-(F-ROLL)-limp,-(M-ROL)-limp). Phagocytic activity of neutrophils was determined by the method opsonophagocytosis reaction using culture of Staphylococcus aureus (strain No. 209) was calculated phagocytic index and phagocytic number.

To rate the impact of all animals was performed needle biopsy tumor formation with subsequent histological and histostereometrically study of the material obtained. Painting of the drugs was performed using hematoxylin and eosin according to Romanovsky-from the Institute.

For (vistastartmenu) evaluation of volumetric ratios of the structural components of the studied tissues and their States used a grid with 100 points. Separately counted the number of tumor cells that are in a stable condition and in a state of degenerative changes and necrosis, lymphoid elements and stroma. When the calculations take into account the number of grid points, with uchino coinciding with each of the above types (States) cells. The results were expressed in percentage. For each drug to ensure the accuracy of the result by the given method the count was carried out ten times.

3-7 days after completion of the course the impact of ZIYA EMF animals was carried out surgical excision of tumor of the mammary glands. Received surgical material were subjected to histological and histostereometrically studies, and the studies were conducted in different parts of the tumor (to assess the impact of different values of the magnetic field).

The impact ZIYA EMF in tumors was determined by comparison of the results of histological studies and vistastartmenu irradiated and non-irradiated tumor tissues. Also a comparison was made of the effects on tumor and healthy tissue. Was also conducted qualitative comparison with the morphological effects after chemotherapy.

The accuracy of the data of clinical, laboratory and sistematicheskikh studies were evaluated by standard methods of mathematical statistics for each defined indicator (characteristic) with confidence level P<0,05.

In dogs with spontaneous malignant neoplasms of the mammary glands to justify this method was performed dynamic study of heme is ideological indicators (up, in the middle and at the end of the course the impact of ZIYA EMF) in comparison with the same indicators in the control group (health dogs without exchange rate effect). The data obtained are shown in table 2.

Table 2
Characteristic hematological parameters when exposed ZIYA EMF with the following parameters: B=(40÷80) MT; tand=0,75 s; tp=0.5 s; Teffects=(30 min·20 sessions) dynamics; (M±m)
IndexThe control groupUntil of course impactIn the middle of the course impactAfter course impacts
Erythrocytes, million/µl4,19±0,224,91±0,484,99±0,654,11±0,55
Hemoglobin, g/l133,69±3,85134,76±7,14139±6,38120,2±9,07
Leukocytes, thousand/ál7,78±0,368,95±0,94*7,52±1,06*6,47±1,17*
Eosinophils, thousand/ál0,48±0,040,43±0,120,42±0,130,38±0,10
Neutrophils p/I, thousand/ál0,14±0,030,12±0,09 0,34±0,130,10±0,08
Neutrophils with/I, thousand/álof 5.05±0,116,33±0,54*6,99±0,65*4,92±0,49*
Lymphocytes, thousand/ál1,94±0,111,99±0,25*1,63±0,27*1,21±0,12*
Monocytes, thousand/ál0,18±0,030,08±0,050,13±0,060,09±0,07
ESR, mm/hour2-84,84±1,476,33±2,496,40±2,37
* - p<0,05

From the table 2 it is seen that there are changes in some hematological parameters:

the decrease in leukocyte count (thousand/ál) to the end of the course impact (8,95±0,94) to (6,47±1,17), which is 28%;

- decrease in the number of lymphocytes (thousand/ál) to the end of the course impact (1,99±0,25) to (1,21±0,12), which was 39%;

- decrease in the number of neutrophils with/I (K/µl) to the end of the course impact (6,33±0,54) to (4,92±0,49), which amounted to 22%.

Comparison of biochemical parameters in blood dynamics in dogs with spontaneous malignant neoplasms of breast (before, middle and after the course of action) with the same parameters in dogs (without impact) character is used in the following changes, presented in table 3.

Table 3
Characterization of biochemical parameters of blood when exposed ZIYA EMF with parameters: impact=(40÷80) MT; tand=0,75 s; tp=0.5 s; Tvozdeistvia=(30 min·20 sessions) dynamics; (M±m)
IndexThe control groupMalignant neoplasm
Until of course impactAfter course impacts
Total protein, g/l55,00-75,0079,48±4,9279,75±3,43
Total bilirubin, µmol/l0,90-or 10.605,5±1,01of 5.83±0,57
Glucose, mol/l3,40-6,004,43±0,614,08±0,82
Urea, mol/l3,10-8,30? 7.04 baby mortality±1,246,63±1,99
Creatinine, µmol/l50,00-110,00103,54±9,31*117,97±8,23*
* - p<0,05

From table 3 it can be seen that changes in performance are insignificant and are within normal limits, except for the values for creatinine. His number had increased the camping to the end of the course impact (103,54± 9,50) µmol/l to (117,97±8,23) µmol/l, which is 14%.

Was conducted and the dynamic monitoring of immunological indices in dogs with spontaneous malignant neoplasms of the mammary glands during the study (before and after exposure) compared with similar indices in the control group (dogs without exchange rate effect). The data shown in table 4.

Table 4
Characterization of the immunological parameters when exposed ZIYA EMF with parameters: V=(40÷80) MT; tand=0,75 s; tp=0.5 s; Teffects=(30 min·20 sessions) dynamics; (M±m)
IndexThe control groupMalignant neoplasm
Until of course impactAfter course impacts
In leukocytes, thousand/ál7,78±0,368,95±0,94*6,47±1,17*
In lymphocytes, thousand/ál1,94±0,111,99±0,25*1,21±0,12*
T/1,76±0,141,71±0,352,00±0,28
The LTI17,60±1,8021,82±2,14 23,10±2,20
T-(F-ROLL)-lymphocytes, thousand/ál0,53±0,050,41±0,09*0,28±0,04*
In-(M-ROL)-lymphocytes, thousand/ál0,30±0,020,24±0,06*0,14±0,02*
Phagocytic activity, %26,88±2,3222,71±3,6822,57±2,64
Phagocytic number $ 1,68±0,212,42±0,611,62±0,48
Phagocytic index6,93±1,0710,65±2,697,18±2,12
* - p<0,95

From table 4 it is seen that:

- reducing the number of leukocytes (thousand/ál) to the end of the course impact (8,95±0,94) to (6,47±1,17), which amounted to 28%;

- reducing the number of lymphocytes (thousand/ál) to the end of the course impact (1,99±0,25) to (1,21±0,12), which is 39%;

- reduction of T-(F-ROLL) lymphocytes (thousand/ál) to the end of the course impact (0,41±0,09) to (0,28:g,04), which accounted for 51%;

- decrease-(M-ROL) lymphocytes (thousand/ál) to the end of the course impact (0,24±0,06) to (0,14±0,02), which amounted to 62%.

In animals subjected to a course of local impact ZIYA EMF marked external changes:

- increase the size of tumors due to edematous component is to the end of the course impact (2,5÷ 7) cm to (3,5÷8,5) cm in diameter;

- to the touch, after a course of exposure of the tumor became softer;

- tumors irradiated animals outside the zone of influence ZIYA EMFs, such changes during the course of exposure was not observed.

In histological preparations of spontaneous malignant tumors of the mammary glands without affecting ZIYA EMF (diagnosis - high and usernotification ductal carcinoma) among stroma are defined glandular and glandular cribrosa patterns of polymorphic atypical cells with hyperchromic nuclei, isolated areas dystrophies and foci of necrosis. There is a predominance of epithelial tumor component beneath the stroma.

A comparative histological study of spontaneous malignant tumors of the mammary glands (diagnosed with a moderate and well-differentiated ductal carcinoma) without impact and after impact ZIYA EMI options and modes: V=(20÷40) MT; tand=0,75 s; tp=0,75 s; Tvozdeistvia=(30 min·20 sessions) notes the following features:

- degenerative changes in the epithelial cells in the form of focal diffuse karyopyknosis and karyorhexis, vacuolization cytoplasm to (25÷50)%;

- increase the area of necrosis up to (15÷25)%;

the change in the ratio of stroma - e is filially tumor component in the direction of the stroma;

- moderate focal lymphoid infiltration of the stroma in the form of clusters of reactive lymphocytes;

the appearance of areas of hyalinosis and fibrosis;

- the presence of hemosiderosis in the stroma.

These changes are more pronounced in histological preparations of those tumors, which were subjected to the exchange rate impact ZIYA EMF with the amplitude of induction: V=(40÷80) MT and the exposure time Teffects=(30 min÷40 sessions), with therapeutic pathomorphosis reaches I-II degree. Degenerative changes and necrosis grow and be (40-60)% and (20-30)%, respectively.

At the same time, histological examination of the tissues of healthy mammary glands at all used parameters of influence ZIYA EMF degenerative changes are (1-3)%, necrosis was not found.

Conducted histochemically processing of histological specimens obtained after surgical removal of malignant tumors of the mammary glands in dogs.

Effects of exchange rate exposure ZIYA EMF in the tumor was evaluated according to vistastartmenu histological preparations. The statistical reliability of the results was provided:

- averaged relative number of structural components and their status for each slice of the tumor on different sections of it;

- averaging the relative share of the VA structural components together the same type of tumors under the same conditions of exposure.

Indicators of structural components of spontaneous malignant tumors of the mammary glands of dogs without impact and after impact local ZIYA EMF with parameters V=(40÷80) MT; tand=0,75 s; tp=0.5 s; Teffects=(30 min·40 sessions), selectively presented in tables 5-6.

2
Table 5
Indicators of structural components of spontaneous malignant tumors of the mammary gland without affecting ZIYA EMF
Structural components and their statusField of view (in %)
12345678910
Tumor cells28262928282631293125
Dystrophic altered cells232424452'3
The necrosis.132011101
Lymphoid elements3113122232
Stroma66676665686761636469

Averaged across the fields of view of the ratio of the structural components (table 5.) characterized by the following parameters (figure 1):

- tumor cells - 28,1%;

- dystrophic modified cells of 3.1%;

- necrosis - 1,2%;

- lymphoid elements to 2.0%;

- stroma - 65,6%.

Table 6
Indicators of structural components of spontaneous malignant tumors of the mammary gland after exposure ZIYA EMI exposure parameters=(40÷80) MT; tand=0,75 sec.; tp=0.5 s; Teffects=(30 min·40 sessions)
Structural components and their statusField of view (in %)
12345678910
Tumor cells1512119101211121210
Dystrophic altered cells1212112112
Necrosis0100000100
Lymphoid elements0101001020
Stroma84848888898786868588

Averaged across the fields of view of the ratio of the structural components (table 6.) characterized by the following parameters (figure 2):

- tumor cells to 11.2%;

dystrophic altered cells is 1.4%;

- necrosis - 0,2%;

- lymphoid elements - 0,5%;

- stroma was 86.7%.

Histochemically processing of histological preparations of spontaneous malignant novobrdo the deposits, subjected to rate the impact of local ZIYA EMF. As a result of irradiation ZIYA EMFs occur following structural changes, which are presented in tables 7-8.

* - p<0,05
Table 7
Characterization of the structural components of spontaneous malignant tumors of the mammary glands after a course of treatment for various amplitudes induction MP (tand=0,75 s; tp=0.5 s; Teffects=(30 min·20 sessions); (M±t)
Structural components and their statusNo impact (%) After a course of action with the induction of MP (20÷40) MT (%) After a course of action with the induction of MP (40÷80) MT (%)
Tumor cells28,49±2,08*23,05±2,47*18,58±1,09*
Dystrophic altered cells2,71±0,672,97±1,072,80±0,59
Necrosis0,80±0,35*1,21±0,41*1,46±0,29*
Lymphoid elements1,51±0,421,64±0,661,56±0,31
Stroma66,49±2,16*71,13±2,25*75,60±3,27*

Table 7 shows that when exposed with an increase in the induction of MT in tissues malignant neoplasms are defined by the following changes:

- significant decrease in tumor cells (%) (28,49±2,08) - no impact, to (23,05±2,47) and (18,58±1,09) - by induction MP=(20÷40) MT=(40÷80) MT after a course of exposure, respectively;

the increase of necrosis (%) C (0,80±0,35) - no impact, to (1,21±0,41) and (1,46±0,29) MT - induction MP=(20÷40) MT=(40÷80) MT after a course of exposure, respectively;

- significant increase in stremi (%) (66,49±,16) - without exposure to (71,13±2,25) and (75,60±3,27) - by induction MP=(20÷40) MT=(40÷80) MT after a course of exposure, respectively.

From table 8 it is seen that with increasing exposure effects in tissues malignant neoplasms occur following changes:

- significant decrease in the number of tumor cells (in%) (28,49±2,08) - no impact, to (18,58±1,09) and (15,24±1,33) - at Teffects=(30 min·20 sessions) and Teffects=(45 min·20 sessions), respectively; at Teffects=(30 min·40 sessions) number of tumor cells is reduced to (13,24±3,19);

- significant increase in stroma (%) (66,49±2,16) - no impact, to (75,60�B1; with 3.27) and (79,37±3,91) - at Teffects=(30 min·20 sessions) and Teffects=(45 min·20 sessions), respectively; at Teffects=(30 min·40 sessions) increased stroma reaches (81,01±8,79).

Table 9
Characterization of the structural components of spontaneous malignant tumors of the mammary glands after treatment with different duty cycle impacts (=(40÷80) MT; Tvozdeistvia=(30 min·20 sessions); (M±m)
Structural components and their statusNo impact (%) After course impacts with tand=(10-15)seconds. and tn=(1-2)s (%)After course impacts with tand=0,75 sec. and tn=0.5 sec (in%)
Tumor cells28,49±2,08*23,61±4,5718,58±1,09*
Dystrophic altered cells2,71±0,673,10±2,862,80±0,59
Necrosis0,80±0,35*1,10±2,581,46±0,29*
Lymphoid elements1,51±0,421,71±1,801,56±0,31
Stroma66,49±2,16*70,48±9,07 75,60±3,27*
* - p<0,05

Table 9 shows that when changing the duty cycle in the structures of spontaneous malignant tumors of the mammary glands following changes have occurred:

- reducing the number of tumor cells (in%) (28,49±2,08) - no impact, to (23,61±4,57) and (18,58±1,09) after impact with a duty cycle (tand=(10÷15) s; tp=(1÷2) s) and (tand=0,75 s; tp=0.5 sec), respectively;

- reduction of dystrophic changes of cells (%) (2,71±0,67) - no impact, to (3,10±2,86) and (2,80±0,59) after impact with a duty cycle (tand=(10÷15) s; tp=(1÷2) s and (tand=0,75 s; tp=0.5 sec), respectively;

- increase of necrosis (%) C (0,80±0,35) - no impact, to (1,10±2,58) and (1,46±0,29) after impact with a duty cycle (tand=(10÷15) s; tn=(1÷2) s and (tand=0,75 s; tp=0.5 sec, respectively;

- increase stroma (%) (66,49±2,16) - no impact, to (70,48±9.07) and (75,60±3,27) after impact with a duty cycle (tand=(10÷15) s; tp=(1÷2)s and (tand=0,75 s; tp=0.5 sec, respectively.

Additional assessment of the effectiveness and impact of local ZIYA EMFs on neoplasms of the mammary gland was obtained by comparison of the results of his pic is of edsti with the consequences after chemotherapy. Animal with malignant tumor (histological diagnosis of well-differentiated ductal carcinoma) underwent a course of chemotherapy scheme (Cyclophosphamide, 100 mg intravenously every other day No. 7; Metotrexat 10 mg intravenous drip in 1 and day 8; Fluorouracil

- 500 mg intravenous drip in 1 and day 8).

In tables 6 and 10 presents the corresponding histostereometrically data for this mapping.

Averaged across the fields of view of the ratio of the structural components of the table 10 is characterized by parameters:

- tumor cells with 25.9%;

- dystrophic modified cells of 3.1%;

- necrosis - 1,1%;

- lymphoid elements to 1.9%;

- stroma - 68,0%.

Table 10
Characterization of the structural components of a malignant tumor of the breast affected by polychemotherapy according to the scheme CMF
Structural components and their statusField of view (in %)
12345678910
Tumor cells242421262426283027
Dystrophic altered cells5523212533
Necrosis0070000004
Lymphoid elements1202313313
Stroma70697069667469646663

The results of numerous studies of the impacts ZIYA EMF with induction B=(20÷80) MTL allows to draw the following conclusions:

1. Fundamentally important are first installed selectivity and morphological irreversibility effects on tumor tissue in comparison with similar healthy. Defined therapeutic pathomorphosis of malignant tumors according to their structural characteristics confirm persp is aktivnosti, non-obviousness and the novelty of the use of ZIYA EMF in Oncology practice.

2. Used the suggested modes local ZIYA EMF exposure significantly lead to therapeutic pathomorphosis of I-II degree of spontaneous malignant tumors in experimental animals, which is consistent with the effects after one course of chemotherapy according to standard procedures.

3. Local impacts of intensive ZIYA EMF when used on animals with malignant tumors leads to significantly-reversible changes in clinical laboratory parameters. Collectively, the results of clinical and laboratory studies indicate the possibility of using ZIYA EMF for the effective treatment of malignant tumors in animals.

The proposed method and realized impact of ultralow-frequency alternating pulsed EMFs are promising for use in veterinary Oncology practice both independently and in combination with other treatment methods.

1. The method of treatment of malignant tumors in dogs, including diagnosis, irradiation of the tumor EMF in preparation for removal of a malignant tumor, surgical removal of the tumor and post-operative care of the animal, wherein the animal is mu in the preoperative period carry out the irradiation of the tumor adjustable local alternating ultralow-frequency pulsed electromagnetic field (ZIYA EMF), when the magnetic field B=(20÷80) MT, at the time of pulse tand=0,75 s, pause time tp=0.5 s, with a duration of irradiation session 30-60 min for a period of 20-40 days, and surgery to remove a malignant tumor spend 3-7 days after completion of the course ZIYA EMF exposure.

2. The method of treatment of malignant tumors in dogs according to claim 1, characterized in that in case of relapse after surgery, re-irradiation ZIYA EMFs from two sides of the affected area when the interval between courses of not more than 20 days.



 

Same patents:

FIELD: medicine; medical equipment.

SUBSTANCE: semiconducting thermoelectric thermomagnetic massager contains effecting plate with massage applicators and semiconducting thermoelectric modules individually connected to power supply, vibration element and control unit. Each massage applicator is made of ferromagnetic alloy with half-spherical end rounding off and mounted in thermal contact with first junctions of related semiconducting thermoelectric modules, and, in addition, belted with wires of opposite winding by adjacent massage applicators connecting power supply through control unit and related semiconducting thermoelectric module. Second junctions of module are in thermal contact with integrated air heater. Within massage applicators there are thermocouples connected to control unit, and vibration element s mounted in rod connecting shaft to effecting plate.

EFFECT: enables to extend range of massage products.

2 dwg

FIELD: medicine.

SUBSTANCE: method involves examining somatosensory evoked potentials. Transcranial magnetic stimulation is applied to motor cortex projection and in cervical radices region. The evoked motor responses are recorded and central motor transition time is measured. Short latent somatosensory evoked potentials are recorded and determined in one stage. The central motor transition time being found increased and somatosensory potentials values being within norm, diskectomy or corporectomy is carried out in anterior cervical access. The central motor transition time being within normal limits and somatosensory potentials values being changed, laminoplasty approach is selected.

EFFECT: wide range of means for determining patient treatment tactics.

2 dwg

FIELD: medicine; medical equipment.

SUBSTANCE: device can be used for improving state of health, of physical and functional reserves of human organism. Electromagnet therapy device has high frequency master oscillator, frequency divider, commutator, control device with control organs, modulating frequency number indicator, output amplifiers provided with operation modes switches, inductors and current availability indicator in inductors.

EFFECT: improved efficiency of treatment.

2 cl, 1 dwg, 5 ex

FIELD: medicine, ophthalmology.

SUBSTANCE: it is necessary to carry out structural-resonance electromagnetic therapy upon "REMATERP" device for the period of 43 min in scanning mode N43, 10 procedures/course at a 2-d-long interval during 1 mo. Simultaneously, it is necessary to fulfill antioxidant therapy with preparation "Vitrum Vision" per 1 tablet twice daily for 1 mo. The suggested innovation enables to decrease the percentage of disease progressing nature, decrease the quantity of complicated forms and the percentage of surgical interferences.

EFFECT: higher efficiency of therapy.

2 tbl

FIELD: medical technique.

SUBSTANCE: irradiator can be used in UHF-therapy. Irradiator for influencing of magnetic component of electromagnet field has two frames connected in opposition; ends of frames are connected with capacitors having similar capacitance; frames are turned one from the other. Adjacent sides of frames are open and third capacitor is introduced into breakage to connect both two first frames. Plates of third capacitor are connected with plates of two edge capacitors by crossed conductors. Sum of capacitances of two edge capacitors equals to 0,9-1,1 capacitance of third capacitor.

EFFECT: reduced power of parasitic radiation; improved precision of dosing of therapeutic radiation dose.

1 dwg

FIELD: medicine; physiotherapy.

SUBSTANCE: device has electric signal detector, control unit, amplifier and inductor. Inductor is made flexible in form of rectangle and it can be mounted if gypsum bandage. Electric signal detector is placed onto chest at area of heart's projection. Blood supply in damaged area id improved and effect of medicinal preparations used for treatment is localized.

EFFECT: improved efficiency of treatment.

3 dwg

FIELD: medicine; veterinary; biology.

SUBSTANCE: biological object is subject to action of pulse rotating magnetic field when making biological-physical tests. Device for acting on human organism by magnetic field has current pulse generator, inductor made of flat coils and control unit, which unit allows bringing coils into operation alternately one after another in some period of time. Coils are made in form of regular four-angled truncated pyramid. Control unit is made programmable for change in alternation of switch of as part of coils of conductor and all the coils, and for controlling parameters of generator as well. Programmable control unit allows switching coils in turn, which coils have to be side faces of pyramid, along circle or cross-wise. It also allows switching coils in turn, which coils have to be lower base and two opposite side faces of pyramid. Device provides variety of schemes of influence of magnetic field onto object and reduction in mutual induction effect as well as increase in working area, where magnetic field is quasi-uniform.

EFFECT: improved efficiency.

5 cl, 4 dwg

FIELD: medicine.

SUBSTANCE: method involves exposing instable angina pectoris patient blood samples to THz-band radiation. The radiation used for treating blood sample at molecular radiation and absorption spectrum nitrogen oxide frequency 240 GHz, power density of 1 mW/cm2 during 15 min.

EFFECT: enhanced effectiveness of treatment.

3 tbl

FIELD: medical equipment.

SUBSTANCE: the apparatus has a controlled power source and an inductor enveloping the working cavity. The inductor consists of a magnetic circuit made of rings wound of ships of electrical-sheet steel, and a winding. The winding is laid along the magnetic circuit. Holes are made in the magnetic circuit rings in radial directions, studs are fastened in the holes with the aid of nuts through insulating bushes. The studs are fastened in the framework for changing the clearance between the magnetic circuit rings and the framework. In thickness the rings represent a core of sheet steel, they are tightened by nuts in the points of passage of the studs. The longitudinal components of the framework are made of several sections interconnected by insulating inserts.

EFFECT: simplified construction, enhanced adaptability to manufacture, reduced loss of power and reduced noise at operation of the apparatus.

3 dwg

FIELD: medicine, neurosurgery.

SUBSTANCE: the innovation should be implemented due to laminectomy, preparing the stumps of spinal cord: one should suture up a ferromagnetic tip covered with biocompatible membrane to proximal end of cerebral cerebrospinal cicatrix, moreover, connection between proximal end of cerebrospinal cicatrix and biocompatible ferromagnetic tip should be fulfilled as a single block being behind dura mater, the developed single block should be placed into tubular biocompatible implant to be rigidly fixed towards posterior osseous structures of vertebrae at lesion level; tubular biocompatible implant should be applied along posterior surface of distal end of spinal cord without its compression, moreover, its length should exceed the size of spinal diastasis 2-fold, not less. Periodically, once daily a patient should be placed into solenoid at direct magnetic field of about 0.5-1.0 T intensity, moreover, intensity vector should coincide with cranio-caudal orientation of spinal conducting tract. Then comes dosed traction of proximal end of spinal cicatrix, moreover, the rate of traction of single block ranges about 0.25-0.3 mm daily; spinal traction should be conducted till exceeding regenerate's length against diastasis' length within 1.0-1.5 cm. On achieving a sufficient length of regenerate of proximal end of spinal cord it is necessary to connect them according to "end-to-end" technique that provides directed tissue regeneration.

EFFECT: higher efficiency of therapy.

FIELD: medicine; medical engineering.

SUBSTANCE: method involves acting upon pathological cells and tissues with constant and/or low frequency alternating electromagnetic field of 1 to 100 mTesla units large intensity and frequency of 1 to 1000 Hz. The device has means for producing constant and/or low frequency alternating electromagnetic fields permeating working space.

EFFECT: enhanced effectiveness in treating pathological tissues without harmfully influencing healthy cells.

19 cl, 5 dwg, 5 tbl

FIELD: medical engineering.

SUBSTANCE: device has three concentric inductance coils arranged in mutually perpendicular relation to each other, control unit producing signals for controlling feeding voltage of winding of each inductance coil. The control unit has PC with software installed and interface unit and three-channeled power amplifier. The PC is connected to the power amplifier for controlling voltage on each coil. The power amplifier is used for supplying voltage to coil windings. Coil windings are arranged turn-by-turn in a way that turns of the same number intersect.

EFFECT: enhanced effectiveness of treatment.

5 dwg

FIELD: medicine.

SUBSTANCE: method involves treating organism with rotating magnetic field. Action mode I has magnetic field intensity of 1.7-3.4 mTesla units giving comfortable organism state. Mode II has magnetic field intensity of 2.9-5.8 mTesla units. Mode III has magnetic field intensity of 0.6-2.3 mTesla units giving discomfort organism state. The treatment is spread over the whole organism by applying modes I-III in any succession order with mode I selected at the beginning and end. Exposure time is equal to 5-10 min in each mode depending on initial organism state. The number of sessions is selected from 3 to 20 in manner individual for each organism.

EFFECT: enhanced effectiveness of treatment.

3 dwg, 1 tbl

FIELD: medicine; medical engineering.

SUBSTANCE: method involves applying variable electromagnetic field excited by amplitude-modulated signal having carrier frequency in 100-1000 kHz bandwidth and modulating frequency in 10-8000 kHz bandwidth, and irradiation with low intensity quasi-monochromatic radiation of visible and proximal infrared wavelength bandwidth with small spectrum width. Two action elements are used when performing the treatment. The first one is vaginal action element having inductive transmitter of resonant type emitting electromagnetic oscillations with carrier frequency belonging to 100-1000 kHz bandwidth amplitude-modulated in 10-8000 kHz bandwidth and low intensity quasi-monochromatic radiation of visible and proximal infrared wavelength bandwidth with small spectrum width. The treatment is carried out concurrently and in continuous mode. The device has power supply source, sinusoid signal oscillator and vaginal action element having dielectric casing. The casing encloses inductive transmitter winding. The sinusoid signal oscillator has active oscillator with inductive transmitter winding being concurrently used as inductive element of oscillatory circuit of the active oscillator. Low frequency oscillator is additionally available. Its output is connected to active oscillator circuit via modulator. Control tract has in series connected amplitude detector, DC amplifier and indicating unit. An additional supracutaneous treatment element has active oscillator, inductive transmitter winding concurrently serving as active oscillator inductance element, modulator, which input is connected to low frequency oscillator output and its output is connected to active oscillator circuit. The control tract has in series connected amplitude detector, DC amplifier and indicating unit. M photodiodes are connected in series via timer and acoustic indication element.

EFFECT: enhanced effectiveness of treatment.

5 cl, 4 dwg

FIELD: medicine; medical engineering.

SUBSTANCE: method involves applying electromagnetic field excited with amplitude-modulated signal having carrying frequency of 100-1000 kHz and modulation frequency of 50-8000 Hz. Patient body trunk is subjected to field treatment and to concurrently acting electromagnetic field formed by amplitude-modulated signal having carrying frequency of 100-2000 kHz and modulation frequency of 50-8000 Hz in local mode. Device has power supply source, sinusoid signal oscillator, action element, low frequency source, one or several local action elements and control tracts. A control tract has amplitude detector, DC amplitude and indication unit.

EFFECT: enhanced effectiveness of treatment; accelerated healing process.

4 cl, 3 dwg

FIELD: medical engineering.

SUBSTANCE: device has cylinder cut along a plane passing through its axis and permanent electromagnet. The electromagnet is arranged in a way that one of its poles is in front of the first half of the cylinder and the other one is in front of the second half. Protrusions are available on the internal side of one of cylinder halves and openings are available on the other one. The openings and protrusions match each other in shape.

EFFECT: enhanced effectiveness in treating strictures with gradually increasing pressure without changing instrument; excluded traumatic urethral mucous membrane injuries.

1 dwg

FIELD: medical engineering.

SUBSTANCE: device has power supply source, M-photodiodes, inductive radiator mounted on flat dielectric carcass, active oscillator, modulator and low frequency oscillator circuits. The radiator is mounted in dielectric casing having two parts. Low frequency oscillator has oscillator of continuously variable frequency in frequency bandwidth of 10-8000 Hz. The device has additional control tract. The tract has in series connected amplitude detector, Dc amplifier and indicator light-emitting diode, timer and acoustic indication unit.

EFFECT: wide range of functional applications.

3 dwg

FIELD: medicine.

SUBSTANCE: method involves determining availability of acute or chronic inflammatory process, inflammation form and stage. Acute inflammatory process being detected in paranasal sinuses of exudative form in catarrhal stage, treatment with extraweak electromagnetic field is administered at the following frequencies: 1.5; 2.5; 2.9; 53.5; 57.0; 75.5; 910.0 Hz in combination with quantum therapy in red light matching the electromagnetic field and directed to nose area in frequency, daily, 4-5 sessions per course. Acute inflammatory process being detected in paranasal sinuses of exudative form in serous stage, treatment with extraweak electromagnetic field is administered at the following frequencies: 2.5; 2.9; 1.7; 1.75; 9.4; 57.0; 58.0; 73.5; 522.0; 4900.0 Hz in combination with quantum therapy in blue and green light matching the electromagnetic field and directed to nose area in frequency, daily, 5-6 sessions per course. Acute or chronic inflammatory process being detected in paranasal sinuses of exudative form in purulent stage, treatment with extraweak electromagnetic field is administered at the following frequencies: 2.5; 2.9; 57.0; 58.0; 53.5; 727.0; 787.0; 802.0; 880.0; 11000.0 Hz in combination with quantum therapy in blue, green and red light matching the electromagnetic field and directed to nose area in frequency, daily, 6-7 sessions per course.

EFFECT: enhanced effectiveness of treatment.

FIELD: medical engineering.

SUBSTANCE: device has high frequency active oscillator and emitter designed as oscillatory LC-circuit. Additional double-pin plug, matching four-terminal network, attenuator and diode rectifier are connected in series. Outlet rectifier terminals are connected to the active oscillator circuit. The device operates with the double-pin plug being connected to acoustic three-program wired radio socket.

EFFECT: enhanced effectiveness of blood circulation recovery; improved rheologic blood properties.

2 cl, 1 dwg

FIELD: medicine.

SUBSTANCE: physiotherapeutic apparatus has power supply, case with cap inside which M light-guides of one type and P light-guides of the other type are disposed. Device also inductive radiator with winding disposed onto flat dielectric frame, auto-generator circuit, modulator, low frequency signal generator and control circuit. Base with light-guides is installed in center of frame of inductive radiator. M light-guides are connected in series with power supply by means of first two-terminal two-positioned switch. P light-guides are connected in series with power supply by means of second two-terminal two-position switch. Control circuit has amplitude oscillator connected in series with dc amplifier and light-guide/indicator. Input of control circuit is connected with active oscillator circuit. Power circuits of active oscillator circuits, modulator, low frequency signal oscillator and control circuit are connected with power supply by means of third two-terminal two-position switch.

EFFECT: widened operational capabilities.

3 dwg

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