Modified gum arabic

FIELD: chemistry.

SUBSTANCE: present invention pertains to modified gum arabic, widely used as an emulsifying agent, thickener, coating agent, binder and capsule material. Description is given of water soluble modified gum arabic from Acacia Senegal, with weight-average molecular weight of not less than 0.9 million Da and RMS-radius of circular motion of arabinogalactan-protein from 46.3-138 nm. The proposed gum arabic has high content of arabinogalactan-protein and better emulsification properties, emulsion stability, film-forming, encapsulating and adhesive capabilities.

EFFECT: higher content of arabinogalactan-protein and better emulsification properties, emulsion stability, film-forming, encapsulating and adhesive capabilities.

19 cl, 5 dwg, 10 tbl, 8 ex

 

The technical field

This invention relates to a modified gum Arabic. In particular, the invention relates to the modified gum Arabic, the properties of which, such as emulgiruet, the stability of the emulsion encapsulating ability, adhesiveness, the property of the protective colloid and film-forming ability, improved or enhanced in comparison with natural gum Arabic. More precisely, the invention relates to the modified gum Arabic obtained from gum Arabic, belonging to the species Acacia senegal, modified gum Arabic containing components with a specific molecular weight and having protein distribution, which are suitable for providing uniform excellent emuliruemogo.

The level of technology

Gum Arabic is a natural bleed and have exudate from the stems and branches of plants that belong to the genus Acacia (especially Acacia senegal and Acacia seyal) family Leguminasae. Gum Arabic is highly soluble in water, and its aqueous solution provides high emulgiruet, the emulsion stability, the ability to encapsulate, adhesiveness (stickiness), the property of the protective colloid and the ability of film formation even at low concentrations, so it is widely used as emulsifier, thickener, stabilizer is ora, binder and covering agent.

Gum Arabic is collected in different countries in the Sahara region in Africa, and it has a large change in molecular weight and composition forming its components due to differences in soil and climate of each place of growth and age of the individual tree. For this reason, the function of gum Arabic in its original condition-dependent habitats are heterogeneous, and therefore, the properties of the drug, which is used gum Arabic, are impermanent (Williams, P. A. and Phillips, G. O. (2000) in Handbook of Hydrocolloids, pp.155-168, Editors: Williams, P. A. and Phillips, G. O., Woodhead, London and New York). In this description such gum Arabic is referred to as simply "gum Arabic" or "natural gum Arabic", or "unmodified gum Arabic" as opposed to "modified gum Arabic" according to this invention.

As mentioned above, emulgiruet is one of the useful properties that gum Arabic manifests in a wide variety of products. Various methods have been proposed and investigated to reduce, to the extent possible, changes in emuliruemogo between samples attributed to changes in the properties of natural gum Arabic, and to improve emulgiruet. For example, one method involves the removal of metal ions from gum Arabic with getting the Arabian acid and its thermal modification for the Sovershenstvovanie its emuliruemogo (not passed examination patent publication Japan No. 1990-49001), another method involves modifying gum Arabic having a loss on drying not more than 50 wt.%, by heating it at 60-140°C for not less than 30 minutes, to thereby improve its emulgiruet (not passed examination patent publication Japan No. 2000-166489).

However, these methods are insufficient modifying gum Arabic, in order to achieve the expected emuliruemogo. So still need efficient ways of producing modified gum Arabic having consistent quality and improved emulgiruet. Furthermore, it is necessary to develop a method for the production of modified gum Arabic, which would have improved properties such as emulsion stability, the ability to encapsulate, adhesiveness (stickiness), the property of the protective colloid or the ability of film formation, and the specified emulgiruet, and have uniform quality by reducing the variance between samples of natural gum Arabic.

Documents related to gum Arabic, include the following documents 1-4, which can be listed in addition to the above documents.

Document 1: Mikio Nakamura, Pharmaceutics, Vol.42, No. 1 (1982) pp.25-29.

Document 2: Carbohydrate research, 246 (1993) pp.303-318.

Document 3: WO 02/072862.

Document 4: has not passed the examination of the patent public is of Japan No. 1983-18370.

Brief description of drawings

Figure 1 (a) and (b) shows the chromatogram of sample 1 and sample 1/36 prepared in experimental example 1 was obtained when these samples were subjected to GPC-MALLS, as mentioned in experimental example 1. Figure 1 (A) shows the chromatogram of sample 1 (raw: natural gum Arabic). Figure 1 (B) shows the chromatogram of the sample 1/36 (heat-treated: modified gum Arabic).

Figure 2 shows the chromatogram of a sample of gum Arabic (raw: natural gum Arabic) of type A. seyal, received when he was subjected to GPC-MALLS, as mentioned in experimental example 1.

Figure 3 shows the profile of the UV-absorbance (wavelength: 214 nm) of the samples 2, 2/24 and 2/48 prepared in experimental example 2, obtained on the basis of GPC-MALLS, which shows their protein distribution.

Figure 4 shows the molecular weight distribution of samples 2, 2/24 and 2/48 prepared in experimental example 2, which is obtained on the basis of GPC-MALLS.

Figure 5 shows the ratio of immunological inhibition (%)obtained by an indirect comparative analysis of ELISA using quantifiable antibodies (SYCC7), for sample 3, sample 3/24 and sample 3/48 in experimental example 8.

The invention

The first whole the present invention is to provide the modified gum Arabic having a high emulgiruet, in particular the modified gum Arabic having essentially uniform quality of emuliruemogo. In addition, the invention relates to emulsifier having such a modified gum Arabic.

The second objective of this invention is to provide a modified gum Arabic, any one or more properties of which, such as emulgiruet, the emulsion stability, the ability to encapsulate, the adhesion property of the protective colloid or the ability of film formation, enhanced or improved compared to natural (unmodified) gum Arabic.

The third objective of this invention is to provide a safe subject modified gum Arabic, exhibiting immunological activity, such as a natural (unmodified) gum Arabic, or similar.

Conducted extensive research to achieve the above goals and found that emulgiruet improved by heating natural gum Arabic (Acacia senegal or Acacia seyal) under particular conditions, and that such modified gum Arabic with improved emuliruemogo differs significantly from natural gum Arabic at least srednevekovoi molecular weight and protein distribution. This izobreteny is done on the basis of specified information and contains the following aspects.

Paragraph 1. Water-soluble modified gum Arabic having srednevekovoy molecular weight of not less than 0.9 million, which is obtained by heating gum Arabic from Acacia senegal.

Paragraph 2. Water-soluble modified gum Arabic having srednevekovoy molecular weight of not less than 1.5 million, which is obtained by heating gum Arabic from Acacia senegal.

Item 3. Water-soluble modified gum Arabic having srednevekovoy molecular weight of not less than 2 million, which is obtained by heating gum Arabic from Acacia senegal.

Item 4. Water-soluble modified gum Arabic having content arabinogalactan-protein not less than 17 wt.%, which is obtained by heating gum Arabic from Acacia senegal.

Paragraph 5. Water-soluble modified gum Arabic having srednevekovoy molecular weight of not less than 0.9 million and the content of the arabinogalactan-protein not less than 10 wt.%, which is obtained by heating gum Arabic from Acacia senegal.

Item 6. Water-soluble modified gum Arabic according to claims 1-5, where the difference in the degree of immunological inhibition is within ±10% between the water-soluble modified gum Arabic and the unmodified gum Arabic from Acacia senegal, as measured by indirect comparative analysis of ELISA using TF is the existing quantitative determination of antibodies to gum Arabic.

Item 7. Water-soluble modified gum Arabic having srednevekovoy molecular weight of not less than 2.5 million, which is obtained by heating gum Arabic from Acacia seyal.

Item 8. Water-soluble modified gum Arabic having content proteincoding high molecular weight component is not less than 25 wt.%, which is obtained by heating gum Arabic from Acacia seyal.

Item 9. Water-soluble modified gum Arabic having srednevekovoy molecular weight of not less than 1.5 million and the content proteincoding high molecular weight component is not less than 22 wt.%, which is obtained by heating gum Arabic from Acacia seyal.

Paragraph 10. Water-soluble modified gum Arabic according to any one of claims 7 to 9, where the difference in the degree of immunological inhibition is within ±10% between the water-soluble modified gum Arabic and the unmodified gum Arabic from Acacia seyal, as measured by indirect comparative analysis of ELISA using quantifiable antibodies for gum Arabic.

Paragraph 11. Water-soluble modified gum Arabic according to any one of claims 1 to 6, which is obtained by heating gum Arabic from Acacia senegal at 110°C for not less than 10 hours, or conditions conducive to achieve essentially the same effect the KTA.

Item 12. Water-soluble modified gum Arabic according to any one of claims 7 to 10, which is obtained by heating gum Arabic from Acacia seyal at 110°C for not less than 10 hours, or conditions conducive to achieve essentially the same effect.

Paragraph 13. The method of obtaining modified gum Arabic according to any one of claims 1 to 6, comprising a stage of heating gum Arabic from Acacia senegal at 110°C for not less than 10 hours, or conditions conducive to achieve essentially the same effect.

Paragraph 14. The method of obtaining modified gum Arabic according to any one of claims 7 to 10, comprising a stage of heating gum Arabic from Acacia seyal at 110°C for not less than 10 hours, or conditions conducive to achieve essentially the same effect.

Paragraph 15. Emulsifier containing a modified gum Arabic according to any one of claims 1 to 10 as an active ingredient.

Paragraph 16. The emulsifier according to § 15 containing the modified gum Arabic according to any one of claims 1 to 4 as an active ingredient.

Paragraph 17. New emulsifier containing a modified gum Arabic as an active ingredient, which has protein distribution that is different from that of the natural gum Arabic from Acacia senegal or Acacia seyal, and a higher content of arabinogalactan-protein than in natural gum Arabic from Acacia senegal, iliessa seyal.

Paragraph 18. The emulsion, which is obtained by dispersing and stabilization of the hydrophobic substance in the hydrophilic solvent with the use of the emulsifier according to item 15 or 16.

Paragraph 19. Emulsion on p, which is an emulsion M/or emulsion/M/C.

Paragraph 20. Emulsion on p, where the hydrophobic substance is an edible hydrophobic substance.

Paragraph 21. Emulsion according to any one of p-20, where the hydrophobic substance is at least one substance selected from the group consisting of essential oils, oleomac, Absolutes, flavorants based on oil-based stains, oil, soluble oil, vitamins, unsaturated polybasic18-C22fatty acids, animal and vegetable fats and oils, SAIB and triglycerides6-C12fatty acid.

Paragraph 22. The production method of the emulsion, including the state of dispersion of a hydrophobic substance in a hydrophilic solvent using any emulsifier in item 15.

Paragraph 23. Thickener, mineral agent, a binder and a material for capsules containing the modified gum Arabic according to any one of claims 1 to 10 as an active ingredient.

Paragraph 24. The modified gum Arabic according to any one of claims 1 to 10 to obtain an emulsifier.

Paragraph 25. The modified gum Arabic according to any one of claims 1 to 10 to obtain the emulsion.

Item are modified gum Arabic according to any one of claims 1 to 10 to obtain a thickener, opaque agent, a binder and a material for capsules.

As mentioned above, this invention relates to the modified gum Arabic of the species Acacia senegal or Acacia seyal, which is obtained by processing natural gum Arabic from Acacia senegal or Acacia seyal, respectively. These natural gumarabic of these species have a different molecular weight, different protein distribution and have different properties due to their structural differences. For example, a natural gum Arabic from A. senegal is levogyrate and has specific optical rotation is approximately -30 degrees. On the other hand, natural gum Arabic from A. seyal is Pervouralsk and has a specific optical rotation of about +50 degrees. Additionally, it is known that in comparison with gum Arabic from A. senegal gum Arabic from A. seyal has a lower content of protein nitrogen (nitrogen content), lower viscosity and different composition of sugars.

The best way to carry out the invention

(1) Modified gum Arabic obtained from A. senegal

(1-1) This invention relates to water-soluble modified gum Arabic having srednevekovoy molecular weight of not less than 0.9 million, preferably not less than 1.5 million and more preferably not less than 2 million to the th obtained by heating gum Arabic from Acacia senegal.

Srednevekovoy molecular weight determined using gel chromatography, where three of the detector, i.e. the detector polygonal laser light scattering (MALLS)detector, refractive index (RI) and ultraviolet (UV) detector in the process stream. In this description of the technology of this gel chromatography is referred to as "GPC-MALLS". In accordance with the GPC-MALLS molecular weight is measured by the detector MALLS, weight of each component (the components) is measured by the RI detector and the protein content was measured by UV-detector. Therefore, it is possible to set the molecular weight and composition of the analyzed components without reference to the standard gum Arabic known molecular weight. Detail about the main features and characteristics of the GPC-MALLS see Idris, O. H. M., Williams, P. A., Phillips, G. O., Food Hydrocolloids, 12 (1998), pp.375-388.

Conditions for the GPC-MALLS used in this invention are the following:

- Column: Superose (6HR) (Pharmacia Biotech, Sweden)

- Flow rate: 0.5 ml/min

The solvent for elution: 0.2 M NaCl

- Preparation of sample: the Sample is diluted with a solvent for elution (0.2 M NaCl).

Concentration of sample: 0,4% (wt./about.)

- The injected volume of the sample solution: 100 ál

dn/dc: 0,141

- Temperature: room temperature

Detector: 1. MALLS (polygonal detector is aseania laser light), detector: DAWN DSP (manufactured by Wyatt Technology Inc., USA), 2. the RI detector, 3. UV detector (absorbance at 214 nm)

The conversion of data obtained by GPC-MALLS under the above conditions, with the use of the software, i.e. ASTRA Version 4.5 (Wyatt Technology), can be set each parameter component of gum Arabic, such as srednevekovaja molecular weight, the extraction ratio (wt.%), the value of polydispersity (R) and the root mean square value of the radius of free rotation (Rg). When the data is converted, taking all peaks in the chromatogram obtained using the RI detector, one peak is obtained molecular weight identify as srednevekovoy molecular mass (Mwt) according to this invention (more specifically "Mwtconverted as a single peak). When the point where the graph of RI begins to rise from a baseline of the chromatogram, defined as "a starting point", and the point where the graph of RI decreases and crosses the base line is defined as the "end point", the above-mentioned single peak on the chromatogram indicates the area from the starting point to the end point.

There is no limit for srednevekovoi molecular weight of the modified gum Arabic according to this invention as long as she is not less than 0.9 million, but preferably it is not less than 1.2 million, more prepact the tion of not less than 1.5 million and even more preferably not less than 2 million. There is no specific upper limit for srednevekovoi molecular weight up until modified gum Arabic is soluble in water, but is preferably 2.5 million or less.

Moreover, the modified gum Arabic according to this invention differs in that it has a specified srednevekovoy molecular weight and is soluble in water. "Water-soluble" in this description means that the sample is almost completely dissolves in excess water, regardless of the type of water, such as water, subjected to ion exchange, or yesterady water, or the water temperature up until the gum Arabic is soluble.

Gidrogenizirovannye gum Arabic can not be dissolved in water, even if you add a large amount of water or when heated, and therefore the term "water-soluble" is used in this description to distinguish the modified gum Arabic according to this invention from hydrogenerating gum Arabic, which is insoluble in water. In other words, the modified gum Arabic according to the invention does not include a modified polymer gum Arabic, which is insoluble in water, such as hydrogels, etc.

Moreover, it is preferable that the modified gum Arabic according to this invention has the above srednevekovoy molecular weight is what I soluble in water and is the same or similar remotefilename the gum Arabic on the immunological reactivity. The expression is the same or similar remotefilename the gum Arabic on the immunological reactivity" means that the difference between the degree of immunological inhibition of modified gum Arabic and unmodified gum Arabic from Acacia senegal is within ±10%, as measured by indirect comparative analysis of ELISA using quantifiable antibodies for gum Arabic, such as "SYCC7". [Thurston, M. I. et al., Detection of gum from Acacia seyal and species of combretum in mixtures with A. Senegal using monoclonal antibodies, Food & Agric. Immunol., 10:237-241(1998); Thurston, M. I. et al., Effect of heat and pH on carbohydrate epitopes from Acacia Senegal by specific monoclonal antibodies, Food & Agric. Immunol., 11:145-153(1999)].

The form of the modified gum Arabic is not limited, and it can take any form, including blocks, balls, rough pulverizate (particles, the obtained coarse spray), granules, tablets and powders.

The modified gum Arabic according to this invention can be obtained by heating gum Arabic from Acacia senegal with the help of a thermostat or a heater such as an oven, for example, at 110°C for not less than 10 hours.

Unmodified gum Arabic (A. senegal), used as starting material in the present embodiment, is a natural bleed and have exudate obtained from the stems and branches of Acacia senegal kind of Acacia family Leguminasae or any other tree, belong to easigo to the same genus. It is also possible to use unmodified gum Arabic, which has been subjected to processing, such as a cleansing treatment, demineralization treatment, spraying or spray drying, etc.

Unmodified gum Arabic (A. senegal) produced in the countries of Northern and Western Africa from Ethiopia to Senegal, Ethiopia, Sudan, Senegal, Nigeria, Niger and Ghana), East Africa such as Kenya and Uganda, the region Sahara Africa and in the pool tributaries of the Nile. Unmodified gum Arabic (A. senegal)obtained in any of these areas can be used in this invention, regardless of its origin.

Moreover, unmodified gum Arabic (A. senegal) is not specifically limited in content in the water. The water content in commercially available unmodified gum Arabic (A. senegal) is reduced when it is dried by heating at 105°C for 6 hours (loss on drying), usually not more than 40 wt.%, preferably not more than 30 wt.% and more preferably not more than 20 wt.%. In this invention, unmodified gum Arabic (A. senegal), with a residual water content or showing a decrease in water content (loss on drying), can be used without restrictions.

Unmodified gum Arabic (A. senegal) usually can be provided in any form blocks, W is Rykov, rude pulverization, granules, tablets and powders (including spray dried powder). However, in this invention, unmodified gum Arabic (A. senegal) any shape can be used without limitation as the source material for processing. You can use a spray dried powder of gum Arabic having a mean particle diameter of from several tens μm to several hundreds μm. There is no specific upper limit for the average particle diameter, but, from the point of view of efficiency modifications, the average particle diameter is preferably not more than 100 mm, the Average particle diameter is preferably in the range from 1 mm to 100 mm and more preferably in the range from 2 mm to 50 mm

Examples of methods of heating unmodified gum Arabic (A. senegal) include heating unmodified gum Arabic at 110°for more than 10 hours with the use of the furnace (thermostat), as mentioned above. The preferred heat treatment is such that when the unmodified gum Arabic (A. senegal) is heated for a period of not less than 15 hours, more preferably for not less than 24 hours and more preferably for not less than 48 hours. Depending on the type of unmodified gum Arabic (A. senegal), which must be subjected to heat the OIC processing, the upper limit of the duration of heating, when it occurs at 110°may be about 72 hours. Until it is possible to obtain a modified gum Arabic, which has a molecular mass of, a precisely defined in this invention, and as long as it is soluble in water, the method of heating is not limited to these examples, and the temperature of the heating, the duration of heating means for heating and heating conditions (relative humidity, open or closed system) can be selected. The effects of the present invention achieved by the heat treatment under the conditions listed above can be also achieved by a method, where the unmodified gum Arabic is heated at a temperature lower than 110°for more than 10 hours or at a temperature higher than 110°for a shorter time. Especially as one example of the first of them can be mentioned a method where the unmodified gum Arabic is heated at 80°within from 3 days to 1 week or more long-term. When unmodified gum Arabic is heated using microwave radiation instead of the furnace, the same effects can be achieved in a shorter time. In addition, heat treatment in the absence of oxygen, such as in conditions of displacement with nitrogen, is desirable because it can is predotvratite discoloration of the gum Arabic.

(1-2) This invention relates to water-soluble modified gum Arabic, which contains not less than 17 wt.% arabinogalactan-protein, which is obtained by heating gum Arabic from Acacia senegal.

Arabinogalactan-protein (referred to hereafter simply as "AGP") is one of the three main components contained in the gum Arabic along with arabinogalactan (AG) and glycoprotein (GP). Unmodified gum Arabic (A. senegal) usually contains the AGP in the relative amount of 5-15 wt.%.

The content of AGP in gum Arabic (unmodified gum Arabic and modified gum Arabic) can be determined by GPC-MALLS, as described above. In particular, when the schedule RI chromatogram obtained using the RI detector, divided into two parts, i.e. peak 1 (high-molecular fraction)which detects the first loireau part, and peak 2 (low molecular weight fraction), which detects later loireau part and then convert using software ASTRA Version 4.5 (Wyatt Technology)received the recovery factor of the peak 1 (wt.%) in line with the AGP (wt.%) in gum Arabic. This is explained in detail with reference to the chromatogram (Figure 1(A))shows the results when the unmodified gum Arabic (A. senegal) analyzed by GPC-MALLS. On the RI chromatogram point, where g is Afik RI begins to rise from a baseline of the chromatogram, defined as "a starting point", and the point where the graph of RI decreases and crosses the base line is defined as the "end point". Between the initial point and the end point of the point where the value of RI shows the minimum, is defined as the boundary, and the area between the starting point and the boundary is defined as peak 1 and the area between the border and the end point is defined as peak 2.

The content of AGP in a modified gum Arabic according to this invention are not particularly limited as long as it is not less than 17 wt.%, but preferably it is not less than 20 wt.%. Its upper limit is not specifically limited as long, while the modified gum Arabic is soluble in water, but is typically about 30 wt.%.

The modified gum Arabic according to this invention differs in that it has the content AGP within the specified limits and is soluble in water. Moreover, it is preferable that the modified gum Arabic according to this invention contains the AGP in the specified quantity, is soluble in water and has properties that are the same or similar properties of unmodified gum Arabic on the immunological reactivity. "Water-soluble" and "properties, the same or similar properties of unmodified gum Arabic on the immunological reactivity", described here, have the same values, is to set in (1-1).

The form of the modified gum Arabic according to this invention are not specifically limited, and it can take any form, including blocks, balls, rough pulverizate, granules, tablets and powders (including spray dried powder).

As described above, the modified gum Arabic according to this invention can be obtained by heating unmodified gum Arabic (A. senegal) using a thermostat or heater such as an oven, for example, at 110°C for not less than 10 hours. As examples of unmodified gum Arabic (A. senegal) as the source material for recycling can be used above and in the specific ways heat can also be used with the methods specified above.

(1-3) This invention relates to water-soluble modified gum Arabic, which has srednevekovoy molecular weight of not less than 9,0×105(0.9 million) and AGP in an amount not less than 10 wt.%, which is obtained by heating unmodified gum Arabic from Acacia senegal.

Srednevekovaja molecular weight is preferably at least 10,0×105(1.0 million), more preferably at least 12,0×105(1.2 million), even more preferably at least 15,0×105(1.5 million), and even more prefer is Ino at least 20,0× 105(2.0 million). Its upper limit is not specifically limited as long, while the modified gum Arabic is soluble in water, but preferably is approximately 25,0×105(2.5 million) or less.

The content of AGP in a modified gum Arabic is preferably not less than 15 wt.%, more preferably not less than 17 wt.% and even more preferably not less than 20 wt.%. Its upper limit is not specifically limited as long, while the modified gum Arabic is soluble in water, but is preferably about 30 wt.% or less.

The modified gum Arabic according to this invention differs in that it has these properties and is soluble in water. Moreover, it is preferable that the modified gum Arabic according to this invention has the above srednevekovoy the molecular weight and the content of the AGP, being soluble in water, and has properties that are the same or similar properties of unmodified gum Arabic on the immunological reactivity. "Water-soluble" and "properties, the same or similar properties of unmodified gum Arabic on the immunological reactivity", described here, have the same meanings as set forth in (1-1).

The form of the modified gum Arabic according to this invention does not specifically limit the I, and it can take any form, including blocks, balls, rough pulverizate, granules, tablets and powders (including spray dried powder).

As described above, the modified gum Arabic according to this invention can be obtained by heating unmodified gum Arabic (A. senegal) using a thermostat or heater such as an oven, for example, at 110°C for not less than 10 hours. As examples of unmodified gum Arabic (A. senegal) for processing can be used above and in the specific ways heat can also be used in the methods above.

(2) Modified gum Arabic obtained from Acacia seyal

(2-1) This invention relates to water-soluble modified gum Arabic having srednevekovoy molecular weight of not less than 25,0×105(2.5 million), which is obtained by heating gum Arabic from Acacia seyal.

In this invention srednevekovaja molecular weight of the modified gum Arabic is not specifically limited as long as it is in the above range, and preferably it is not less than 26,0×105(2.6 million), and even more preferably not less than 30,0×105(3.0 million). The upper limit of its srednevekovoi molecular weight is not limited to,until modified gum Arabic is a water-soluble, but preferably it is about 40,0×105(4.0 million).

The modified gum Arabic according to this invention differs in that it has a specified srednevekovoy molecular weight and is soluble in water. Moreover, it is preferable that the modified gum Arabic according to this invention has the above srednevekovoy molecular weight, being soluble in water, and has properties that are the same or similar properties of unmodified gum Arabic on the immunological reactivity. "Water-soluble" and "properties, the same or similar properties of unmodified gum Arabic on the immunological reactivity", described here, have the same meanings as set forth in (1-1).

The form of the modified gum Arabic according to this invention are not specifically limited, and it can take any form, including blocks, balls, rough pulverizate, granules, tablets and powders (including spray dried powder).

The modified gum Arabic according to this invention can be obtained by heating unmodified gum Arabic (A. seyal) using a thermostat or heater such as an oven, for example, at 110°C for not less than 10 hours.

Unmodified gum Arabic (A. seyal), used as starting material in the present embodiment, is of genuine cowhide leather-makes the m bleed and have exudate from the stems and branches of Acacia seyal kind of Acacia family Leguminasae or any other plants, belonging to the same genus. It is also possible to use unmodified gum Arabic, which has been subjected to processing, such as a cleansing treatment, demineralization treatment, spraying or spray drying, etc.

Unmodified gum Arabic (A. seyal) is traditionally produced in the countries of Northern and Western Africa from Ethiopia to Senegal, Ethiopia, Sudan, Senegal, Nigeria, Niger and Ghana), East Africa such as Kenya and Uganda, the region Sahara Africa and in the pool tributaries of the Nile. Unmodified gum Arabic (A. seyal)obtained in any of these areas can be used as source material for modification according to this invention, regardless of its origin.

Moreover, unmodified gum Arabic (A. seyal) is not specifically limited in content in the water. Any commercially available unmodified gum Arabic (A. seyal) can be used, regardless of water content.

Unmodified gum Arabic (A. seyal) can usually be provided in any form, such as blocks, balls, rough pulverizate, granules, tablets and powders (including spray dried powder). Unmodified gum Arabic (A. seyal) of any shape can be used without limitation as a source of material which must be subjected Teplov the th treatment according to this invention. You can use spray dried gum Arabic having a mean particle diameter of from several tens μm to several hundreds μm. There is no specific upper limit for the average particle diameter, but, from the point of view of efficiency modifications, the average particle diameter is preferably not more than 100 mm, the Average particle diameter is preferably in the range from 1 mm to 100 mm and more preferably in the range from 2 mm to 50 mm

Examples of methods of heating unmodified gum Arabic (A. seyal) include heating unmodified gum Arabic at 110°C for not less than 10 hours with thermostat or heater, as mentioned above. The preferred heat treatment is such that when the unmodified gum Arabic (A. seyal) is heated at 110°C for not less than 15 hours, more preferably for not less than 24 hours and more preferably for not less than 48 hours. Depending on the type of unmodified gum Arabic (A. seyal), which must be subjected to heat treatment, the upper limit of the duration of heating, when it occurs at 110°With, may be, for example, about 72 hours. Until then, while it is possible to obtain a modified gum Arabic, which has a molecular weight exactly certain this is sobienie, and while it is soluble in water, the method of heating is not limited to these examples, and the temperature of the heating, the duration of heating means for heating and heating conditions (relative humidity, open or closed system) can be selected. The effects of the present invention achieved by the heat treatment under the conditions listed above can be also achieved by a method, where the unmodified gum Arabic is heated at a temperature lower than 110°for more than 10 hours or at a temperature higher than 110°for a shorter time. Especially as one example may be mentioned a method of heating unmodified gum Arabic at 80°within from 3 days to 1 week or more long-term. When unmodified gum Arabic is heated using microwave radiation instead of the furnace, the same effects can be achieved in a shorter time. In addition, heat treatment in the absence of oxygen, such as in conditions of displacement with nitrogen, is desirable because it can prevent discoloration of the gum Arabic.

(2-2) This invention relates to water-soluble modified gum Arabic, which contains more than 25 wt.% contains protein of high molecular weight component, which receive heating the receiving of gum Arabic from Acacia seyal.

The content contains protein of high molecular weight component in an unmodified gum Arabic from Acacia seyal and modified gum Arabic from it may be determined by GPC-MALLS, as described above. In particular, when the schedule RI chromatogram obtained using the RI detector, divided into two parts, i.e. peak 1 (high-molecular fraction)which detects the first loireau part, and peak 2 (low molecular weight fraction), which detects later loireau part and then convert using software ASTRA Version 4.5 (Wyatt Technology)received the recovery factor of the peak 1 (wt.%) consistent with the content (wt.%) contains protein of high molecular weight component in the gum Arabic. This is explained in detail with reference to the chromatogram (Figure 2)shows the results when the unmodified gum Arabic (A. seyal) analyzed by GPC-MALLS. On the RI chromatogram point where the graph of RI begins to rise from a baseline of the chromatogram, defined as "a starting point" and the point where the graph of RI decreases and crosses the base line, defined as "end-point". Between the initial point and the end point of the point where the value of RI shows the minimum, is defined as the boundary, and the area between the starting point and the boundary is defined as peak 1 and the area between the border and the end point is defined as p is 2.

As for Acacia senegal, contains protein of high molecular weight component (peak 1) is one of the main components contained in the unmodified gum Arabic (A. seyal). Unmodified gum Arabic (A. seyal) usually contains the specified component in the range from 10 to 24 wt.%.

The content contains protein of high molecular weight component (peak 1) in the modified gum Arabic (A. seyal) this invention are not particularly limited as long as it is within the specified limits. However, preferably it is not less than 26 wt.% and more preferably not less than 30 wt.%. Its upper limit is not specifically limited as long, while the modified gum Arabic is a water-soluble, however, it is preferable that the content contains protein of high molecular weight component comprises about 45 wt.% or less.

The modified gum Arabic (A. seyal), obtained according to this invention differs in that it has content containing protein macromolecular component within the specified limits and is soluble in water. Moreover, it is preferable that the modified gum Arabic according to this invention contains contains protein of high molecular weight component at the specified relative amount, being soluble in water, and has properties that are the same or podobnyesayty unmodified gum Arabic (A. seyal) on the immunological reactivity. "Water-soluble" and "properties, the same or similar properties of unmodified gum Arabic (A. seyal) on the immunological reactivity", described here, have the same meanings as set forth in (1-1).

There are no limitations on the form of the modified gum Arabic (A. seyal) according to this invention, and it can take any form, including blocks, balls, rough pulverizate, granules, tablets and powders (including spray dried powder).

As described above, the modified gum Arabic according to this invention can be obtained by heating unmodified gum Arabic (A. seyal) using a thermostat or heater such as an oven, for example, at 110°for more than 10 hours. As examples of unmodified gum Arabic (A. seyal), which should be modified, can be used above and in the specific ways heat can also be used in the methods above (see (2-1)).

(2-3) This invention relates to water-soluble modified gum Arabic, which has srednevekovoy molecular weight of not less than 15,0×105(1.5 million) or more and the content contains protein of high molecular weight component is not less than 22 wt.%, which is obtained by heating nimodipine is consistent gum Arabic from Acacia seyal.

Srednevekovaja molecular weight water-soluble modified gum Arabic (A. seyal) is preferably at least 20,0×105(2.0 million), and more preferably at least 25,0×105(2.5 million). Its upper limit is not specifically limited as long, while the modified gum Arabic (A. seyal) is soluble in water, but usually it is, for example, about 40,0×105(4.0 million) or less.

The content contains protein of high molecular weight component (peak 1) in the modified gum Arabic (A. seyal) is preferably not less than 25 wt.% and more preferably not less than 30 wt.%. Its upper limit is not specifically limited as long, while the modified gum Arabic is soluble in water, but usually it is, for example, about 45 wt.% or less.

The modified gum Arabic (A. seyal), obtained according to this invention differs in that it has these properties and is soluble in water. Moreover, it is preferable that the modified gum Arabic according to this invention has srednevekovoy the molecular weight and the content contains protein of high molecular weight component being soluble in water, and has properties that are the same or similar properties of unmodified gum Arabic on the immunological reactivity. Soluble in water, and properties, the same or similar properties of unmodified gum Arabic (A. seyal) on the immunological reactivity", described here, have the same meanings as set forth in (1-1).

The form of the modified gum Arabic (A. seyal) this invention are not specifically limited, and it can take any form, including blocks, balls, rough pulverizate, granules, tablets and powders (including spray dried powder).

As described above, the modified gum Arabic according to this invention can be obtained by heating unmodified gum Arabic (A. seyal) using a thermostat or heater such as an oven, for example, at 110°C for not less than 10 hours. As examples of unmodified gum Arabic (A. seyal), which should be modified, can be used above and in the specific ways heat can also be used in the above methods (see (2-1)).

The modified gum Arabic according to this invention, especially the modified gum Arabic, isolated from species A. senegal, represented by any one of items (1-1) to (1-3), can be clearly distinguished from the unmodified gum Arabic in emuliruemogo. The modified gum Arabic, especially the modified gum Arabic, isolated from species of A. senegal has a higher emulgiruet than it is deficieny gum Arabic.

The modified gum Arabic according to this invention, especially the modified gum Arabic, isolated from species A. seyal, of any of items (2-1) to (2-3), can be clearly distinguished from the unmodified gum Arabic in emuliruemogo, stability of the emulsion, the ability to encapsulate, adhesion (stickiness), property, protective colloid or the ability of film formation. The modified gum Arabic has improved emulgiruet, the emulsion stability, the ability to encapsulate, the adhesion property of the protective colloid or the ability of film formation compared with those properties of unmodified gum Arabic.

Emulgiruet modified gum Arabic, especially the modified gum Arabic, isolated from species A. senegal, can be estimated by measuring the mean diameter of the emulsion droplets (dispersed phase), when the emulsion is prepared with its use. It is preferable that the average diameter of the droplets of the emulsion prepared using the modified gum Arabic, is, 21, usually not more than 1 μm, preferably not more than 0.8 μm, more preferably not more than 0.7 μm and even more preferably not more than 0.6 μm. The emulsion for use in this assessment can be prepared according to the method described is in experimental example 1 (2).

In addition, it is preferable that the emulsion is stable in time. This emulsion stability over time can be assessed by measuring the mean diameter of the particles of the emulsion immediately after preparation [average particle diameter (a)] and the average particle diameter of the emulsion in a few days (2-7 days) of storage at 60° [average particle diameter (b)], respectively, and determine the difference between the two values [(b)-(a)]. Although it is not limited to, in the case of storage of the emulsion within 7 days at 60°preferably, this difference is equal to 1 μm or less, more preferably 0.3 μm or less and even more preferably of 0.1 μm or less.

(3) Modified gum Arabic according to this invention, especially the modified gum Arabic, isolated from species A. senegal, suitable as emulsifier for the preparation of a variety of emulsions, particularly emulsions of the type oil-in-water (M/V), or emulsions In/M/in food, pharmaceutical drugs, medicine, quasilogarithmic, perfumes, cosmetics and other fields. The modified gum Arabic is extremely applicable as emulsifier for the preparation of products that take by mouth, such as food products, pharmaceuticals and quasilocality. This invention relates to emulsifier containing specified Modific the integration of gum Arabic as an active component.

More specifically, the modified gum Arabic according to this invention can be suitably used as an emulsifier to emulsify foods or their constituents, such as confectionery (for example, sweets, chewing gum, cough medicine, diabetes tablets, chewable tablets, chewing candy, lozenges, tablets sweets, dry snacks etc), dairy products or frozen foods (e.g., ice cream, ice sherbets, bottled milk and cream and so on), bakery products, beverages (for example, liquid drinks, powdered drinks, desserts, processed fish products, processed livestock products, food products that have undergone a heat treatment, etc.; coatings for food or pharmaceutical products such as tablets, etc.; flavorant or cosmetic products oil-based, or based stains, oils etc.

Specified modified gum Arabic as such can be used without additives as emulsifier in the form of a solution, in granular form or in powder form, however, it is also possible to prepare it for use as an emulsifier adding other carriers and/or additives in accordance with the usual methods in these areas, if necessary. In this case, the media and DOB the key can be suitably selected in accordance with a customary method using natural gum Arabic as emulsifier in these areas depending on the type and purpose of the product, which must be emulsified. For example, to the modified gum Arabic can be added dextrin, maltose, lactose and the like saccharide or glycerol, propylene glycol and similar polyhydric alcohols. In this embodiment as a reference can be used to document 1 (Roy L. Whistlerand James N. BeMiller, INDUSTRIAL GUMS - Polysaccharides and Their Derivatives", SECOND EDITION, ACADEMIC PRESS, New York and London, 1973, pp.197-263) and document 2 (Martin Glicksman "Gum Technology in the Food Industry" ACADEMIC PRESS, New York and London, 1969, pp.94-124).

(4) the invention additionally relates to a method of preparation of the emulsion, using the specified modified gum Arabic, isolated from A. senegal. This emulsion can be prepared by dispersing and stabilization of the hydrophobic substance, which is dispersible substance in the hydrophilic solvent with the use of this modified gum Arabic as emulsifier. Examples of the emulsion is shown in this invention include emulsions of the type oil-in-water (M/V) or emulsion In a/M/C.

The hydrophobic substance to be emulsified according to this invention, is not specifically limited as long as it is a substance that can usually be included as part of the emulsion or should be processed in the emulsion; however, the preferred hydrophobic substances that are used in the field of PI is of evich products pharmaceuticals, quasilegal or fragrances and cosmetics, and hydrophobic substances that can be taken through the mouth, for example, particularly preferred edible hydrophobic substance.

Specific examples include essential oils extracted from plant sources, such as citrus plants, such as orange, lime, lemon, grapefruit, etc.; olomola isolated from plant sources, such as pepper, cinnamon, ginger, etc.; Absolutes extracted from plant sources, such as Jasmine, rose, etc.; flavorant-based oils, such as synthetic flavorant oil-based and mixed flavorant based oils etc; dye-based oils, such as β-carotene, the dye of paprika, lycopene, the palm oil carotene, carotene Donalliella, carrot carotene, etc.; soluble in oil vitamins such as vitamin a, D, E and K; polybasic unsaturated fatty acids, such as C18-C22polybasic unsaturated fatty acids, including unsaturated polybasic fatty acids n-6 (linoleic acid, γ-linolenic acid and arachidonic acid, etc.) and polybasic unsaturated fatty acids n-3 (α-linolenic acid, docosahexaenoic acid and eicosapentanoic acid etc); animal and vegetable fats and oils, such as soybean oil, rapso the second oil corn oil and fish oil; SAIB (mixed acetate-isobutyrate sucrose), food technology oil, such as C6-C12triglycerides of medium chain length, and optional mixtures of such food materials based on oil.

The method of preparation of the emulsion is not specifically limited, and may include the stage of mixing a hydrophobic substance and a hydrophilic solvent in the presence of modified gum Arabic according to the standard method of preparation of emulsions of the type oil-in-water (M/V), or emulsions In/M/, preferably mechanical agitation, for example, by using a homogenizer or the injection system high pressure. In particular, the following process can be described as an example.

First, the modified gum Arabic dissolved in a hydrophilic solvent such as water, and, if necessary, impurities are removed by appropriate means separation of solid and liquid, such as centrifugation or filtration with a filter press or the like, receiving an aqueous solution of gum Arabic. Target the hydrophobic substance (for example, oil or fat or a mixture obtained by dissolving flavorant or dye in such oil or fat) is mixed with the obtained aqueous solution of gum Arabic with the help of a mixer for pre-emulsification. During this process the Rel the relative density can be adjusted by means of an agent, regulating the relative density, such as SAIB. The pre-emulsion thus obtained, then emuleret using equipment for emulsification.

Examples of applicable hydrophobic substances include such substances. However, when emulsified flavorant or emulsified dye must be prepared using flavorant or dye-based oils, it is preferable to use a mixture of solutions, where flavorant or dye or oil-based pre-dissolved in the oil or fat as a hydrophobic substance. This gives the possibility to stabilize the emulsion and prevent evaporation of the components. Oils and fats in which such flavorant or dye-based oil needs to be diluted, is not specifically limited, but usually can be used triglycerides medium chain length (C6-C12triglycerides of fatty acids and vegetable oils such as corn oil, safflower oil and soybean oil.

There are no restrictions on used equipment for emulsification, and it may be suitably selected in accordance with the size of the droplets of the target emulsion and the viscosity of the material. For example, can be used homogenizer pressure homogenizer and other equipment for emulsification, such as m linica Disper Mill, colloid mill, etc.

The emulsification can be carried out by adding a hydrophobic substance to the hydrophilic solvent during mixing, the implementation of pre-emulsification to obtain an emulsion having a particle diameter of 2-5 μm, and processing it in the equipment for emulsification, such as a homogenizer to obtain an emulsion having fine and uniform particles (e.g., average particle diameter not more than 1 μm).

Many dyes, including β-carotene, exist in the form of crystalline suspensions. Therefore, to handle such dyes in the emulsion (emulsified dye), preferably in advance to mix and dissolve the crystals in a suitable oil or fat at a high temperature and then add the resulting solution in a hydrophilic solvent.

Compared with the emulsion prepared using natural (unmodified) of gum Arabic, the emulsion prepared using the modified gum Arabic according to this invention has a uniform particle diameters and is very stable, so that substantially prevents coagulation or merge emulsified particles caused by harsh treatment or exposure to severe conditions such as heat, prolonged storage, etc

(5) Modificarea the hydrated gum Arabic according to this invention, especially gum Arabic, isolated from A. seyal, can be suitably used as a thickener, binder, opaque agents, suspendida agents, agents for sizing and finishing, material for capsules (encapsulant) and others in the production of food, pharmaceuticals, quasilocal, flavorants/cosmetics, inks, paints, adhesives, lithographs, textiles because of its improved and enhanced emuliruemogo, stability of the emulsion, the ability to encapsulate, adhesion (stickiness), the properties of the protective colloid and the ability of film formation. Therefore, this invention relates to a thickener, binder, opaque agent and material for capsules (encapsulant), where specified, the modified gum Arabic, especially the modified gum Arabic (A. seyal), serves as the active component. Thickener, binder, opaque agent or material for capsules (encapsulant) is prepared according to a conventional method in this field except for using the modified gum Arabic according to this invention as the active component.

In this embodiment as a reference can be used to document 1 (Roy L. Whistlerand James N. BeMiller, INDUSTRIAL GUMS - Polysaccharides and Their Derivatives", SECOND EDITION, ACADEMIC PRESS, New York and London, 1973, pp.197-263) and document 2 (Martin Glicksman "Gum Technology i the Food Industry" ACADEMIC PRESS, New York and London, 1969, pp.94-124).

EXAMPLES

The invention will be described below in detail with reference to the following experimental examples and examples, but it is not limited to these examples. In each example, unless otherwise specified, "part" means "part by weight" and "%" means "% by mass". In each composition the substance marked with a "*"means the product of San-Ei Gen F.F.I. Inc.

Experimental example 1

Modification of gum Arabic and evaluation of modified gum Arabic

70 kg of crushed gum Arabic (A. senegal: sample 1) (particle size 5 mm) loaded into a stainless steel drum with a volume of 100 l and then heated at 110°within 36 hours of receiving the sample 1/36". For carrying out chromatography those samples of gum Arabic (sample 1 and sample 1/36) is subjected to GPC-MALLS under the following conditions.

<Conditions GPC-MALLS>

- Column: Superose (6HR) 10/30 (Pharmacia Biotech)

- Flow rate: 0.5 ml/min

The solvent for elution: 0.2 M NaCl

- Sample preparation: samples for analysis of dissolved solvent for elution (0.2 M NaCl)

Concentration of sample: 0,4% (wt./about.)

- Load volume of sample: 100 ál

dn/dc: 0,141

- Temperature: room temperature

Detector: (1) the detector MALLS (multi-angle scattering of laser light): DAWN DSP (Wyatt Technology Inc.),

(2) the RI detector (figure Prelom the tion),

(3) UV detector (absorbance at 214 nm)

Chromatogram obtained from sample 1 and sample 1/36, shown in figure 1 (a) and (b), respectively. "Volume (ml)", corresponding to the abscissa shows the total volume of eluting solvent passing through the column, and "AUX, 90° detector", corresponding to the ordinate shows the relative intensity of each detector (MALLS detector, detector RI and UV detector). The chromatogram (figure MALLS), obtained using the detector MALLS, shows the intensity of light scattering at 90°, which correlates with the molecular weight distribution. The RI chromatogram (schedule RI)obtained with the RI detector, shows the intensity of the refractive index, which correlates with the mass of a component contained in each eluate. UV-chromatogram (UV graph) shows the UV absorbance at 214 nm, which correlates with the protein distribution.

On the basis of the schedule RI is obtained from RI detector, eluruume components can be classified into two factions, loireau fraction of high molecular weight components, which eluted first (peak area 1, shown in figure 1), and loireau fraction of low molecular weight components, which eluted later (peak area 2, shown in figure 1). More specifically, the point where the graph of RI (curve RI) begins to rise from a baseline chromate is grams, defined as "a starting point" and the point where the graph of RI (curve RI) falls and crosses the base line is defined as the "end point". The point where the intensity of the RI indicates the minimum between the initial point and the end point, defined as the boundary. The peak area between the starting point and the boundary is specified eluruumi fraction of high molecular weight components (peak area 1) and the peak area between the border and the end point is specified eluruumi fraction of low molecular weight components (peak area 2).

Elwira fraction of high molecular weight components (peak area 1) is the fraction containing the highest protein and its extraction ratio (wt.%) corresponds to the contents of the arabinogalactan-protein (AGP) in gum Arabic. The comparison between figure 1 (A)showing the elution profiles of gum Arabic (A. senegal) (sample 1)and figure 1 (B)showing the elution profiles heated gum Arabic (A. senegal) (sample 1/36), shows the following.

Elwira fraction protein component of high molecular weight (AGW) (peak area 1) in sample 1 (unmodified gum Arabic) found low measured value with detector light scattering detector (MALLS), monitored at 90° (peak height: about 1.3), low measured value RI (small quantity) and the extended UV absorption. On the contrary, elwira who may fraction protein component of high molecular weight (AGW) (peak area 1) in the sample 1/36 (heated gum Arabic) shows a high measured value with a MALLS detector (peak height: about 8), high intensity RI (a large number) and a sharp peak of UV absorption.

Srednevekovoy molecular weight, the extraction ratio (wt.%), the value of polydispersity (R) and the root mean square value RMS radius of free movement (Rg) obtained by converting the data obtained under these conditions with the use of the software ASTRA Version 4.5 (Wyatt Technology).

"Srednevekovoy molecular mass (Mwt)" (more precisely, Mwtconverted as a single peak)used in this invention, is defined as the molecular weight obtained when all peaks in the chromatogram obtained with the RI detector, is considered as one peak in the data conversion. These all peaks mean that they are represented by the area from the starting point to the end point, when the point where the graph of RI (curve RI) begins to rise from a baseline of the chromatogram RI, is defined as "a starting point", and the point where the graph of RI (curve RI) falls and crosses the base line is defined as the "end point". The extraction ratio (wt.%) the peak area 1 shows the contents of the AGP in the Arabic gum (natural gum Arabic (A. senegal), modified gum Arabic (A. senegal)). RMS is the radius of the free movement (Rg) is used as an indicator of molecular size. The value of Rg corresponds to the molecular weight, and therefore increased MOLEKULYaRNAYa corresponds to high value of Rg. The value of polydispersity (R) is defined as the ratio srednevekovoi molecular mass (Mwto srednetsenovoj molecular mass (Mn) [i.e., (Mw/Mn)]. When the value of R is high, the peaks in the chromatogram RI becomes wide, which indicates that the molecular weight has a high variability (polydispersity) (peaks of different molecular masses are present as mixed). When the value of R is low, the peaks in the chromatogram RI are sharp, which indicates that the polydispersity is low.

These parameters define the two types of data conversion: the conversion of data as a single peak of all peaks in the chromatogram obtained with the RI detector, and data conversion as two peaks with the schedule, divided into loireau fraction of high molecular weight components, which eluted first (peak area 1), and loireau fraction of low molecular weight components, which eluted later (peak area 2), as shown in figure 1 (a) and (B). The results are shown in table 1.

Table 1
Options converted as a single peakOptions converted as two peaks
Education is EC Mwt1)wt.%PRgMwtwt.%PRg
1are 5.36±0,02×1051012,0120,42,30±0,01×106< / br>
3,74±0,01×105
8,12)< / br>
92,9
1,19

1,56
34,0

18,9
1/361,97±0,04×106101to 4.6233,69,44±0,25×106< / br>
5,16±0,03×105
16,12)< / br>
84,9
1,79

the 1.44
54,5

27,7
1)Srednevekovaja molecular mass of the sample of gum Arabic.

2)The content of the arabinogalactan-protein sample of gum Arabic (% by weight).

These results show that heat treatment of gum Arabic it srednevekovaja molecular weight increases from about are 5.36×105(sample 1) to about 1,97×106(sample 1/36) and AGP increases from about 8.1% (sample 1) to 16.1% (sample 1/36).

(2) Evaluation of emulsifying ability

The emulsion is prepared using the specified samples of gum Arabic (sample 1 and sample 1/36), according to the mode p is epigenome below, and emulgiruet each sample estimate, determining the average particle diameter and the storage stability of the emulsions.

More specifically, the samples (sample 1 and sample 1/36), each dissolved in water, centrifuged to remove insoluble substances, and prepare aqueous solutions of 7.5%, 10%, 15% and 20% of gum Arabic. To 800 g of each of these solutions add 200 g triglycerides of medium chain length (triglyceride octane/decanoas acid O.D.O. (trademark, product of Nisshin Oil Mills, Ltd.)) under stirring and the mixture emuleret using a homogenizer (manufactured by APV Gaulin) (homogenized 4 times at a pressure of 44 MPa (450 kg/cm2)), obtaining the emulsion. The average particle diameter of the obtained emulsions was measured immediately after emulsification and after 2 days of storage at 60°using analyzer distribution of particle size (laser diffraction analyzer particle size SALD-1100, manufactured by Shimadzu).

As a rule, emulgiruet emulsifier is excellent, as the average particle diameter of the emulsion prepared using the emulsifier, and less particle diameter remains more stable over time ("Research method turbidimetric analysis of emulsion M/, emulsified with gum Arabic", Yakugaku Zasshi (Pharmacology Journal, 112(12) 906-913 (1992)).

Average particle diameter and the storage stability of e is ulsi, prepared with each sample of gum Arabic (sample 1 and sample 1/36) shown in table 2. The storage stability can be determined on the basis of the difference [(b)-(a)] between the average particle diameter of the emulsions immediately after emulsification (a) and the average particle diameter of the emulsions after accelerated testing (2 days storage at 60° (C) (b).

Table 2
Water< / br>
the solution of gum Arabic
The average particle diameter of emulsion (μm)
Sample 1Sample 1/36
Immediately after< / br>
emulsification
After the accelerated test< / br>
(2 days store< / br>
at 60°C)
ChangeImmediately< / br>
after< / br>
emulsification
After the accelerated test< / br>
(2 days store< / br>
at 60°C)
Change
7,50,98to 3.732,750,820,830,01
100,882,952,070,720,730,01
0,742,101,360,650,660,01
200,681,540,860,630,640,01

The smaller the average particle diameter of the emulsion, the better the storage stability of the emulsion. The conclusions that can be made when considering the average particle diameter of the emulsion, the following:

"Excellent emulgiruet": when the average particle diameter of the emulsion is less than 0.1 microns;

"Moderately good emulgiruet": when the average particle diameter of the emulsion is in the range from 0.1 μm to 1 μm, and

"Bad emulgiruet": when the average diameter of the emulsion particles is 1 μm or more.

As can be seen from table 2, in the sense of emuliruemogo, unmodified gum Arabic (sample 1) was poor, while the heated gum Arabic (sample 1/36) was excellent, as the average particle diameter was less than 0.1 ám.

Experimental example 2

Modification of gum Arabic and evaluation of modified gum Arabic

1 kg of crushed gum Arabic, belonging to the species Acacia senegal (natural gum Arabic from A. senegal: 'sample 2') (particle size 5 mm), placed in an unpressurized container is er stainless steel, open for access of air, and heated at 110°within 24 hours, and 48 hours using an oven (samples of gum Arabic is heated for 24 hours and 48 hours, referred to as 'sample 2/24' and 'sample 2/48', respectively). These samples of gum Arabic (sample 2, sample 2/24 and sample 2/48) is subjected to GPC-MALLS and the chromatogram receive the same manner as in experimental example 1. Srednevekovoy molecular weight, the extraction ratio (wt.%), the value of polydispersity (P) and the RMS radius of the circular motion (Rg) obtained by converting the received data using the software ASTRA Version 4.5 (Wyatt Technology). These parameters define the two types of data conversion: the conversion of data as a single peak of all peaks in the chromatogram obtained with the RI detector, and data conversion as two peaks with the schedule, divided into loireau fraction of high molecular weight components, which eluted first (peak area 1), and loireau fraction of low molecular weight components, which eluted later (peak area 2). The results are shown in table 3.

Table 3
Options converted as a single peakThe parameters of preobrazovan the e as two peaks
SampleMwt1)wt.%PRgMwtwt.%PRg
24,13±0,15×1051062,1428,11,87±0,19×106< / br>
3.04 from±0,11×105
7,382)< / br>
98
1,23

1,67
34,4

24,7
2/248,62±0,36×1051162,9936,43,52±0,14×106< / br>
3,97±0,16×105
17,32)< / br>
97
1,56

1,58
42,3

24,7
2/481,43±0,06×1061023,6842,5of 5.29±0,23×106< / br>
4,79±0,18×105
20,22)< / br>
82
1,76

1,49
46,3

29,6
1)Srednevekovaja molecular mass of the sample of gum Arabic.

2)The content of the arabinogalactan-protein sample of gum Arabic (% by weight).

Profile UV absorbance (wavelength: 214 nm)showing the protein distribution of each sample is shown in figure 3, and so the fil, showing the molecular weight distribution of each sample shown in figure 4. Figure 3 on the X-axis plotted the cumulative amount of the eluate from the column (ml) (volume (ml)) and Y-axis plotted the relative intensity of the UV response at a wavelength of 214 nm (LS, AUX (volts)).

The molecular weight of each sample of gum Arabic (sample 2, sample 2/24 and sample 2/48) at the maximum point of the RI curve (Mp/RImax) and at the maximum point of the UV-curve (Mp/UVmax) are shown in table 4.

Table 4
SampleMp/RImaxVolume (ml)RgMp/UVmaxVolume (ml)Rg
21,74±0,06×1066,008the 33.41,06±0,03×1066,66729,9
2/243,01±0,06×1065,842of 37.9of 4.38±0,01×1065,60840,4
2/483,97±0,12×1065,817of 40.99,86±0,33×1065,51744,5

These results show that heat treatment of gum Arabic it is srednevekovaja molecular weight increases from about 4,13× 105(sample 2) to about 8,62×105(sample 2/24) or about 1,43×106(sample 2/48) and its content AGP increases from about 7,38% (sample 2) to 17.3% (sample 2,24) or 20.2% (sample 2/48).

(2) Evaluation of emulsifying ability

The emulsion is prepared using the specified samples of gum Arabic (sample 2, sample 2/24 and sample 2/48), according to the method below, and the average particle diameter and the storage stability of the emulsions were determined to assess emulgiruet each sample.

More specifically, 1 kg of each of the obtained samples (sample 2, sample 2/24 and sample 2/48) dissolved in 4 kg of water, centrifuged to remove insoluble substances, and prepared 20% aqueous solution of each sample of gum Arabic. To 850 g of 20% aqueous solution of each sample, add 150 g triglycerides of medium chain length (triglyceride octane/decanoas acid O.D.O. (trademark, product of Nisshin Oil Mills, Ltd.)) under stirring, and each mixture emuleret using a homogenizer (manufactured by APV Gaulin) (homogenized 4 times at a pressure of 44 MPa (450 kg/cm2)), obtaining the emulsion. The average particle diameter of the obtained emulsions was measured immediately after emulsification and after 7 days storage at 60°using analyzer distribution of particle size (laser diffraction analyzer particle size SALD-1100, manufactured by Shimadzu).

Average particle diameter and the storage stability of emulsions prepared with each sample of gum Arabic (sample 2, sample 2/24 and sample 2/48), shown in table 5. The storage stability is determined on the basis of the difference [(b)-(a)] between the average particle diameter of the emulsions immediately after emulsification (a) and the average particle diameter of the emulsions after the accelerated test (7 days storage at 60° (C) (b).

Table 5
SampleGroup< / br>
emuliruemogo
The average particle diameter of the emulsion immediately after< / br>
emulsification< / br>
(mm) [a]
The average particle diameter of the emulsion after the accelerated test (7 days at 60°C)< / br>
(mm) [b]
Changing the diameter of the particles< / br>
(ám) [(b)-(a)]
20,632,121,49
2/24And0,510,510
2/48And0,510,510

The above table shows that the smaller the change in the size of the droplets of the emulsion (average particle diameter), the better the storage stability. Emulsifying ways the activity of samples classified into 3 groups based on changes in average particle diameter (the size of the droplets of the emulsion) as follows:

"Excellent emulgiruet (group a): when the average particle diameter less than 0.1 microns;

"Moderately good emulgiruet (group b): when the average particle diameter of the emulsion is in the range from 0.1 μm to 1 μm, and

"Bad emulgiruet (group C): when the average particle diameter is 1 μm or more.

Therefore, as shown in table 5, unmodified gum Arabic (sample 2) are referred to the group as showed poor emulsifying ability, whereas the heat treated samples of gum Arabic, and the sample 2/24, and the sample 2/48 assigned to group a, as demonstrated excellent emulsifying ability. There are relationships between properties and emuliruemogo gum Arabic, which are listed in the following items (1)to(4), on the basis of figure 3, showing the profile of the UV-absorption, figure 4, showing the molecular mass profile, and tables 3 and 4, showing the various parameters, which are shown by comparing the heat-treated samples 2/24 and 2/48 in group a (excellent emulsifying power) with the sample 2 in the group (bad emulsifying ability).

(1) When srednevekovaja molecular weight increases, emulgiruet improved. Specifically srednevekovaja molecular weight of the modified gum Arabic should be preferably 9 million or more, more preferably 1.2 million or more, even more preferably 1.5 million or more and more preferably 2 million or more, from the point of view of emuliruemogo.

(2) When the content of the AGW increases, emulgiruet improved. Specifically, the content of the AGP should be preferably 12% or more, more preferably 17% or more and more preferably 20% or more from the point of view of emuliruemogo.

(3) When the molecular weight at the maximum point of the RI curve (Mp/RImax) and molecular weight at the maximum point of the UV-curve (Mp/UVmax) become higher, emulgiruet improved. In particular, the molecular weight preferably 4000000 or more at the maximum point of the UV-curve (Mp/UVmax), from the point of view of emuliruemogo.

(4) When the form of the first peak in the UV-graph becomes more sharp, emulgiruet getting better.

This process (heat treatment) allows redistribution of proteins natural gum Arabic (in other words, allows you to change the protein distribution gum Arabic), to increase the content of AGP, which improves emulgiruet.

Experimental example 3

1 kg of crushed gum Arabic (A. senegal: 'sample 3') (particle size 5 mm) is heated at 110°within 24 hours, and 48 hours using an oven, in the same manner as the experimental example 2. Samples of gum Arabic is heated for 24 hours and 48 hours, referred to as 'sample 3/24' and 'sample 3/48', respectively. These samples of gum Arabic (sample 3, sample 3/24 and sample 3/48) is subjected to GPC-MALLS, and chromatogram receive the same manner as in experimental example 1. Various parameters (srednevekovoy molecular weight, the extraction ratio (wt.%), the value of polydispersity (P) and the RMS radius of the circular motion (Rg)) is obtained by converting the received data in the same manner as in experimental example 1. Srednevekovaja molecular mass (Mwtconverted as a single peak) samples of gum Arabic (sample 3, sample 3/24 and sample 3/48) shown in table 6.

Table 6
SampleMwtconverted as a single peak1)weight%
35,15±0,18×105101Control
3/241,15±0,21×106105Heated at 110°within 24 hours
3/481,91±0,17×106103Heated at 110°within 48 hours
1)srednevekovaja molecular mass of the sample of gum Arabic.

Experimental example 4

1 kg of spray dried gum Arabic (A. senegal: 'sample 4') (powdered form) is heated at 110°within 24 hours, using a microwave (gum Arabic is heated for 24 hours is referred to as 'sample 4/24'), in the same manner as in experimental example 2. These samples of gum Arabic (sample 4 and sample 4/24) is subjected to GPC-MALLS, and chromatogram receive the same manner as in experimental example 1. Various parameters (srednevekovoy molecular weight, the extraction ratio (wt.%), the value of polydispersity (P) and the RMS radius of the circular motion (Rg)) is obtained by converting the received data in the same manner as in experimental example 1. The results are shown in table 7.

Table 7
Options converted as a single peakOptions converted as two peaks
SampleMwt1)wt.%PRgMwtwt.%PRg
45,99±0,2×105107 2,24252,34±0,09×106< / br>
3,19±0,08×105
14,552)< / br>
br93.1
1,49

of 1.34
35,2

-
4/241,43±0,26×106102of 4.6697,66,38±0,91×106< / br>
3,82±0,08×105
182)< / br>
83,3
2,78

1,45
109

19
1)Srednevekovaja molecular mass of the sample of gum Arabic.

2)The content of the arabinogalactan-protein sample of gum Arabic (% by weight).

Experimental example 5

1 kg of spherical clusters of gum Arabic (A. senegal: 'sample 5') (cluster size of 20 mm × 30 mm or less) is heated at 110°within 24 hours, using a microwave (gum Arabic is heated for 24 hours is referred to as 'sample 5/24'), in the same manner as in experimental example 2. These samples of gum Arabic (sample 5 and sample 5/24) is subjected to GPC-MALLS, and chromatogram receive the same manner as in experimental example 1. Various parameters (srednevekovoy molecular weight, the extraction ratio (wt.%), the value of polydispersity (P) and the RMS radius of the circular motion (Rg)) is obtained by converting the received data in the same manner as in experimental is the detailed example 1. The results are shown in table 8.

Table 8
Options converted as a single peakOptions converted as two peaks
SampleMwt1)weight%PRgMwtweight%PRg
58,05±0,44×105992,4834,13,20±0,18×106< / br>
3,95±0,04×105
13,62)< / br>
85,5
1,55

1,39
49,7

-
5/241,63±0,28×1061074,731245,88±1,1×106< / br>
4,06±0,32×105
23,42)< / br>
84,5
2,43

1,48
138

21
1)Srednevekovaja molecular mass of the sample of gum Arabic.

2)The content of the arabinogalactan-protein sample of gum Arabic (% by weight).

Experimental example 6

Crushed gum Arabic (A. seyal: 'sample ') (particle size 5 mm) is heated at 110° C for 15 hours using an oven (gum Arabic, heated for 15 hours, referred to as 'sample 6/15'), in the same manner as in experimental example 2. These samples of gum Arabic (sample 6 and sample 6/15) is subjected to GPC-MALLS, and chromatogram receive the same manner as in experimental example 1. Various parameters (srednevekovoy molecular weight, the extraction ratio (wt.%), the value of polydispersity (P) and the RMS radius of the circular motion (Rg)) is obtained by converting the received data in the same manner as in experimental example 1. The results are shown in table 9.

Table 9
Options converted as a single peakOptions converted as two peaks
SampleMwt1)weight%PRgMwtweight%PRg
61,65±0,66×1061051,7727,14,82±0,19×106< / br>
1,10±0,10×106
15,772)< / br>
89,7
1,20

1,33
30,5

24,1
6/153,65±0,17×1061102,7540,2scored 8.38±0,44×106< / br>
1,21±0,04×105
37,22)< / br>
73,2
1,53

1,26
42,5

30,7
1)Srednevekovaja molecular mass of the sample of gum Arabic.

2)The content of the arabinogalactan-protein sample of gum Arabic (% by weight).

Experimental example 7

Dried spray gum Arabic (A. seyal: 'sample 7') (powder form: 2 mm or less) is heated at 110°within 24 hours, using a microwave (gum Arabic, warmed up within 24 hours, referred to as 'sample 7/24'), in the same manner as in experimental example 2. These samples of gum Arabic (sample 7 and sample 7/24) is subjected to GPC-MALLS, and chromatogram receive the same manner as in experimental example 1. Various parameters (srednevekovoy molecular weight, the extraction ratio (wt.%), the value of polydispersity (P) and the RMS radius of the circular motion (Rg)) is obtained by converting the received data in the same manner as in experimental example 1. The results are shown in table 10.

Table 10
Options converted as a single peakOptions converted as two peaks
SampleMwt1)weight%PRgMwtweight%PRg
71,15±0,04×1061072,8036,44,72±0,33×106< / br>
5,85±0,14×105
10,92)< / br>
96,7
1,73

1,56
47,8

13,2
7/241,46±0,26×1061043,1351,56,15±0,57×106< / br>
3,82±0,08×105
22,92)< / br>
82
1,81

1,47
60,3

18,5
1)Srednevekovaja molecular mass of the sample of gum Arabic.

2)The content of the arabinogalactan-protein sample of gum Arabic (% by weight).

Experimental example 8

The immune reaction of the modified gum Arabic

Evaluate the immunological reactivity of each of the samples of gum Arabic from A. senegal (sample 3, sample 3/24 and sample /48), obtained in experimental example 3. More specifically immunological reactivity of each of the samples of gum Arabic measured using tablets, immobilized each gum Arabic (concentration: 0,005, 0,01, 0,05, 0,1, 0,5, 1, 5 mg/ml)by indirect comparative analysis by ELISA according to Thurston, M. I. et al [Thurston, M. I. et al., Detection of gum from Acacia seyal and species of combretum in mixtures with A. senegal using monoclonal antibodies, Food & Agric. Immunol., 10:237-241 (1998); Thurston, M. I. et al., Effect of heat and pH on carbohydrate epitopes from Acacia senegal by specific monoclonal antibodies, Food & Agric. Immunol., 11:145-153 (1999)].

Method ELISA described below.

Get a monoclonal antibody, having specificity to any kind of gum Arabic and showing quantitative cross-reactivity. More specifically adjuvant added to the physiological solution containing 1 mg/ml gum Arabic from Acacia seyal, to get the immunogen. Mice Balb/c do intraperitoneally injections of immunogen three times with 2-week intervals. Splenocytes of mice with greatly increased titers of antibodies are selected and combined with the myeloma cells in polyethylene glycol. After incubation of the received cells on incubation tablet for 10 days cells hybridoma selected on the basis of the specificity of the antibodies produced in the supernatant proliferating cells hybridoma. The selected cells hybridoma incubated for 10 days at incubatio the nom tablet and specific cell hybridoma selected by the same procedure. Cell hybridoma, producing only antibodies SYCC7 without specificity to any kind of gum Arabic and showing quantitative cross-reactivity eventually selected.

Each of the solutions of 1 mg/ml and 5 mg/ml for the three samples (sample 3, sample 3/24 and sample 3/48) was diluted 10, 100 and 1000 times. 200 µl of each solution is added to the wells of plastic tablet and immobilized at 4°C for 1 hour. The wells are washed with saline solution, the block containing 0.3% casein saline solution and washed containing 0.05% Tween 20 saline solution. Add the culture supernatant obtained cells hybridoma and immobilized for 1 hour. After washing, as in the above procedure, the wells sequentially immobilized with peroxidase labeled goat artemisinine antibody (SIGMA, diluted 1000 times with saline) for 1 hour. After washing the wells, add tetramethylbenzidine as substrate, and the color intensity measured by the UV absorbance at 450 nm (UV450 nm). The ratio of inhibition (%) for each sample at various concentrations denote by comparing the UV absorbance at 450 nm (UV450 nm) natural gum Arabic from A. seyal, which is taken as 100% inhibition.

The results are shown in figure 5. Accessiblestateset concentration (mg/ml) of each sample of gum Arabic, used for coating tablets, and the ordinate represents the ratio of inhibition (%). The test results show that the modified gum Arabic according to this invention and a natural gum Arabic have the same or similar immunological reactivity as differences in the coefficient of immunological inhibition between them are within ±10% at all tested concentrations and, therefore, small.

Example 1

Emulsion β-carotene (emulsified preparation of dye)

Using the samples heat-treated gum Arabic (Acacia senegal), obtained in experimental examples 1-6, as samples of the modified gum Arabic, prepare emulsions β-carotene according to the following composition.

<Composition>

30% suspension β-carotene5
The triglyceride of medium chain length10
The modified gum Arabic (Acacia senegal)17
Water68
Total100 (mass%)

More specifically, 170 g of the modified gum Arabic (Acacia senegal) is dissolved in 680 g of water, and the solution centrifuged to remove insoluble substances, thus obtaining 20% in the command solution of modified gum Arabic. The solution of gum Arabic is used as emulsifier, and to the solution was added a mixed solution obtained by dissolving 100 g triglycerides of medium chain length (triglyceride octane/decanoas acid O.D.O. (trademark, product of Nisshin Oil Mills, Ltd.)) 50 g of a 30% suspension β-carotene when heated at 150aboutWith subsequent mechanical agitation. The mixture emuleret homogenizer (manufactured by APV Gaulin) (homogenized 4 times at a pressure of 44 MPa (450 kg/cm2))to obtain emulsion β-carotene, which serves as an emulsified preparation of dye.

Example 2

Emulsion orange flavorant (emulsified flavorant)

Using the samples heat-treated gum Arabic (Acacia senegal), obtained in experimental examples 1-6, as samples of the modified gum Arabic, prepare emulsions orange flavorant according to the following composition.

<Composition>

Orange flavorant2 (mass%)
The triglyceride of medium chain length13
The modified gum Arabic (Acacia senegal)17
Water68
Total100 (mass%)

More specifically 170 g modificirovana what about the gum Arabic (Acacia senegal) is dissolved in 680 g of water, and the solution centrifuged to remove insoluble substances, thus obtaining a 20% aqueous solution of modified gum Arabic. The solution of gum Arabic is used as emulsifier, and to the solution was added a mixed solution obtained by mixing sufficiently 20 g orange flavorant and 130 g triglycerides of medium chain length (triglyceride octane/decanoas acid O.D.O. (trademark, product of Nisshin Oil Mills, Ltd.)) at room temperature, followed by mechanical stirring. The mixture emuleret homogenizer (manufactured by APV Gaulin) (homogenized 4 times at a pressure of 44 MPa (450 kg/cm2))to obtain emulsion orange flavorant that serves as the emulsified flavorant.

Example 3

Emulsion docosahexaenoic acid (DHA) (emulsified drug DHA)

Using the samples heat-treated gum Arabic (Acacia senegal), obtained in experimental examples 1-6, as samples of the modified gum Arabic, prepare DHA emulsion according to the following composition.

<Composition>

Fish oil containing 20% DHA5 (mass%)
The triglyceride of medium chain length10
The modified gum Arabic (Acacia senegal)17
Water68
Total100 (mass%)

More specifically, 170 g of the modified gum Arabic (Acacia senegal) is dissolved in 680 g of water, and the solution centrifuged to remove insoluble substances, thus obtaining a 20% aqueous solution of modified gum Arabic. The solution of gum Arabic is used as emulsifier, and to the solution was added a mixed solution prepared by mixing 50 grams of fish oil containing 20% DHA, and 100 g triglycerides of medium chain length (triglyceride octane/decanoas acid O.D.O. (trademark, product of Nisshin Oil Mills, Ltd.)) and heating the mixture at 80°With subsequent mechanical agitation. The mixture emuleret homogenizer (manufactured by APV Gaulin) (homogenized 4 times at a pressure of 44 MPa (450 kg/cm2))to obtain emulsion DHA.

Example 4

Lemon powder flavorant

Using the samples heat-treated gum Arabic (Acacia seyal), obtained in experimental example 7, as a sample of the modified gum Arabic, prepare lemon powder flavorant according to the following composition.

<Composition>

tr>
Lemon oil20 (mass%)
The modified gum Arabic (Acacia seyal)20
Dextrin60
Water150
Total250 (mass%)

More specifically, 200 g of the modified gum Arabic (Acacia seyal) and 600 g of dextrin are dissolved in 1500 g of water to obtain an aqueous solution of modified gum Arabic. The solution of gum Arabic is used as an emulsifier. Lemon oil (200 g) are added to a solution of gum Arabic, followed by mechanical stirring. The resulting mixture emuleret homogenizer (manufactured by APV Gaulin) (homogenized 1 times at a pressure of 19.6 MPa (200 kg/cm2)). After that, the solution is spray dried using a spray dryer (manufactured Anhydro) (temperature at the input 140°C, outlet temperature 80° (C)receiving lemon powder flavorant (950 g).

Industrial application

This invention relates to a modified gum Arabic, especially to the modified gum Arabic of the species A. senegal, with improved emuliruemogo. Moreover, this invention can provide a modified gum Arabic, especially the modified gum Arabic of the species A. senegal, which shows a stable and improved emulgiruet by uniform regulation and standardization, particularly in relation to emuliruemogo, changes in kachestve the natural properties of gum Arabic, ascribed to various areas of collection, time and climate. Thus, the modified gum Arabic according to this invention, especially the modified gum Arabic of the species A. senegal, can be suitably used for emulsification of various hydrophobic substances, such as essential oils, dyes or oil-based, flavorant based oil, soluble oil, vitamins, etc. of the Emulsion prepared using the modified gum Arabic according to the invention are more stable as compared to cooked using natural (unmodified) gum Arabic, because the distribution of particles is uniform, and the quality deterioration caused by agglomeration or by integration of the particles of the emulsion under the influence of conditions such as heating or prolonged storage can be significantly suppressed.

This invention relates to a modified gum Arabic, especially to the modified gum Arabic of the species A. seyal, with enhanced and improved emuliruemogo, emulsion stability, ability encapsulation, adhesive property (adhesion), property, protective colloid or the ability of film formation compared to natural (unmodified) gum Arabic. Moreover, this invention can provide modifica is consistent gum Arabic, especially the modified gum Arabic of the species A. seyal, which shows stable emulgiruet, due to uniform regulation and standardization of changes in the quality and properties of gum Arabic, attributed to various areas of collection, time, climate, particularly emuliruemogo, ability encapsulation, adhesion (stickiness), property, protective colloid, the ability of film formation, etc. of Such modified gum Arabic according to the invention can be suitably used for a thickener, a binder, a material for capsules (encapsulant), opaque agent and emulsifier.

1. Water-soluble modified gum Arabic from Acacia senegal, with srednevekovoy molecular weight of not less than 0.9 million and RMS is the radius of the circular motion arabinogalactan-protein from 46.3 to 138 nm.

2. Water-soluble modified gum Arabic according to claim 1, where srednevekovaja molecular weight is not less than 1.5 million Da.

3. Water-soluble modified gum Arabic according to claim 1, where srednevekovaja molecular weight is not less than 2 million Yes.

4. Water-soluble modified gum Arabic according to claim 1, which has a content of arabinogalactan-protein not less than 10 wt.%.

5. Water-soluble modified gum Arabic according to claim 1, where the difference in degree immunologicals the th inhibition is within ± 10% between the water-soluble modified gum Arabic and the unmodified gum Arabic from Acacia Senegal, as measured by indirect comparative analysis of ELISA using quantifiable antibodies for gum Arabic.

6. Water-soluble modified gum Arabic according to claim 1, where the difference between the average diameter of the particles in the emulsion immediately after emulsification and after 2-7 days of storage at 60°With equal less than 0.1 microns.

7. Water-soluble modified gum Arabic from Acacia senegal, with the content of the arabinogalactan-protein not less than 17 wt.% and RMS is the radius of the circular motion arabinogalactan-protein from 42,3 to 138 nm.

8. Water-soluble modified gum Arabic according to claim 7, where the difference in the degree of immunological inhibition is within ±10% between the water-soluble modified gum Arabic and the unmodified gum Arabic from Acacia senegal, as measured by indirect comparative analysis of ELISA using quantifiable antibodies for gum Arabic.

9. Water-soluble modified gum Arabic according to claim 7, where the difference between the average diameter of the particles in the emulsion immediately after emulsification and after 2-7 days of storage at 60°With equal less than 0.1 microns.

10. Emulsifier containing a modified gum Arabic according to claim 1 or 7 as the active component.

11. The emulsion, which is obtained by dispersing and stabilization of the hydrophobic substance in the hydrophilic solvent with the use of the emulsifier of claim 10.

12. The emulsion according to claim 11, which is an emulsion M/or emulsion/M/C.

13. The emulsion according to claim 11, where the hydrophobic substance is an edible hydrophobic substance.

14. The emulsion according to claim 11, where the hydrophobic substance is at least one substance selected from the group consisting of essential oils, oleomac, Absolutes, flavorants based on oil-based stains, oil, soluble oil, vitamins, unsaturated polybasic C18-C22fatty acids, animal and vegetable fats and oils, SAIB and triglycerides6-C12fatty acid.

15. The production method of the emulsion, including the state of dispersion of a hydrophobic substance in a hydrophilic solvent using any emulsifier of claim 10.

16. Thickener, mineral agent, a binder and a material for capsules containing the modified gum Arabic according to claim 1 or 7 as the active component.

17. The modified gum Arabic according to claim 1 or 7 to get emulsifier.

18. The modified gum Arabic according to claim 1 or 7 to obtain the emulsion.

19. The modified gum Arabic according to claim 1 or 7 to get thickener, covering Agen is a, binder and material for capsules.



 

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EFFECT: obtaining oligosaccharides with antithrombotic activity.

28 cl, 6 ex

FIELD: chemistry, biochemistry.

SUBSTANCE: invention relates to biotechnology. The proposed method comprises thermal processing of larva, separated from a substratum upon termination of their cultivation, for 2 to 15 min at 75 to 100°C. Thermal processing over, larvae are crushed, chitine-containing fraction is separated from hemolymph. Now, the chitine-containing precipitate as-washed and crushed is subjected to enzymic hydrolysis at temperature 35 to 46°C with pH of 6.5 to 8.5 till achieving amine nitrogen content not below 0.8 G/%. Then, the separated chitine-containing precipitate is subjected to alkaline hydrolysis at continuous mixing for 60 to 90 min at 55 to 65°C. Finally, the aforesaid chitine-containing precipitate is subjected to demineralisation by 1.5 to 3.0 percent solution of hydrochloric or nitric acid for 30 to 40 min at 60 to 70°C. After that, the precipitate containing chitine is rinsed with water till pH of 6.5 to 7.5 and dried.

EFFECT: increase in biological value of target products with simultaneous increase in their number and simplification of process method.

4 cl, 1 ex

FIELD: chemistry.

SUBSTANCE: used are films made of glycosaminoglycan conjugates with 4- or 5-aminosalicylic acids or alginate conjugates with 4- or 5-aminosalicylic acids or intermixed conjugates or mixed conjugates and at least one polymer chosen from group including carboxymethyl cellulose, glycosaminoglycan, alginate, gelatine, albumin with salicylate content not less than 50%. Specified mixtures are treated with 2-20% iron (III) chloride solution at room temperature within 1-5 minutes. Surface complex lowers water- and biological liquids solubility of the film.

EFFECT: prolonged of biomaterial activity is provided.

10 ex

FIELD: chemistry.

SUBSTANCE: large part of the cells of material, containing non-starch polysaccharides is destroyed in the grinding process, obtaining particles, containing non-starch polysaccharides, which are high solubility when the product gets into contact with dissolving media. The non-starch polysaccharides are contained in particles, obtained by breaking up material until formation of particles with particle size less than the size of the corresponding initial cells of the non-starch polysaccharide. The material can be crushed using a combination of heat, pressure and shearing force. Crushing can be carried out through extrusion, intumescence and homogenisation under pressure. Preferred materials for such processing include oats, rye or their part. Solubility and conditions for dissolving the product can be regulated through mixing the material, subject to crushing, with a component rich in amylopectin. The product, obtained using this method, can be used in food products.

EFFECT: increased solubility of non-starch polysaccharides contained in grinded materials.

15 cl, 4 dwg, 8 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: molar ratio of cyclodextrin to the acid in the complex comprises 1:1. The complex is obtained by introduction of concentrated water cyclodextrin solution heated to boiling point to glacial acetic acid of room temperature, with further separation and drying of crystalline sediment. Complex of α- or β-cyclodextrin with acetic acid is stable in dry state, but in water solution it is decomposed into components. Obtained solution gains properties of dilated acetic acid, and therefore can be used as flavouring in food concentrates, as preservation agent, solution acidity regulator, and as buffer component in biochemistry and analytical chemistry.

EFFECT: enhanced efficiency of composition.

3 cl, 4 ex, 1 tbl

FIELD: chemistry.

SUBSTANCE: invention pertains to modified polysaccharide in particular to modified polysaccharide Neisseria meningitidis of serogroup A, which preserves immunogenicity, but has improved stability. The modified polysaccharide is obtained from reaction of capsular polysaccharide, or its fragment - oligosaccharide, with CDI type bifunctional reagent, accompanied by reaction with an amino-compound, such as dimethylamine. Description is also given of modified polysaccharide conjugates and vaccines, which are obtained from such conjugates.

EFFECT: obtaining modified saccharide.

70 cl, 17 dwg

FIELD: chemistry.

SUBSTANCE: invention relates to pectin and processed initial material, which contains pectin. Method of obtaining processed initial material by deactivation of pectinesterase includes obtaining fruit vegetable material, containing pectin, and bringing it in contact with water, which has pH value from 3.2 to 3.9, at temperature ≤70°C. After that processed initial material is extracted. In addition, invention relates to use of processed initial material, containing pectin, obtained according to said method, respectively, for pectin extraction, as food for animals or as ingredient in food products. In addition invention relates to pectin, obtained according to said method, by means of extraction from initial vegetable material, molecular weight of said pectin being higher on value up to 50% than molecular weight of pectin, obtained from extraction of similar but unprocessed initial vegetable material, containing pectin. Ratio of sensitivity to calcium of said pectin and sensitivity to calcium of pectin, extracted from similar, but unprocessed with washing, initial vegetable material, which contains pectin, is within 0.90-1.40. Pectin preserves molecular weight and etherification degree well, has high output and low sensitivity to calcium.

EFFECT: obtaining method of processing vegetable material, which contains pectin, preserving molecular weight and degree of pectin etherification.

30 cl, 2 dwg, 19 tbl, 9 ex

FIELD: chemistry.

SUBSTANCE: invention concerns aldehyde derivatives and conjugates of di-, oligo- or polysaccharide, of the general formula (I), methods of obtaining them, and pharmaceutical composition based on them and capable of staying in blood flow for prolonged time. , where R is -CH(CHO)CH2OH, -CH2CHO, -CH(CH2NHR1)CH2OH, -CH(CH2NHNHR1)CH2OH, -CH(CH=NNHR1)CH2OH, -CH2CH2NHR1, -CH2CH=N-NHR1, -CH2CH2NHNHR1; R1 is polypeptide or albumen; GlyO is a sialic acid bond; R3 is H; R4 is OH; n is 2 or more.

EFFECT: obtaining pharmaceutical composition based on aldehyde derivatives of sialic acid capable of staying in blood flow for prolonged time.

20 cl, 7 tbl, 22 dwg, 10 ex

FIELD: chemistry.

SUBSTANCE: invention describes a method of epi-K5-N-sulfate oversulfation for obtaining epi-K5-amine-O-oversulfate with very high sulfation degree, which produces new epi-K5-N,O-oversulfate derivatives with sulfation degree of 4-4.6 on following N-sulfation, the derivatives being almost inactive to fibrillation parametres and applicable in pharmaceutical compositions with antidermatitis and antiviral effect. The invention also describes new low-molecular epi-K5-N-sulfates applicable as transit products in obtaining the respective low-molecular epi-K5-N,O-oversulfate derivatives.

EFFECT: method of epi-K5-N-sulfate oversulfation for obtaining epi-K5-amine-O-oversulfate with extremely high sulfation degree.

55 cl, 4 dwg

FIELD: petroleum processing.

SUBSTANCE: invention relates to reagents stabilizing phase composition of emulsions with high water content in petroleum product, e.g. spent mineral oil. Water-oil emulsion stabilizer is based on polymer material, which is, in particular, petroleum polymer resin obtained via polymerization of dicyclopentadiene fraction of straight-run gasoline pyrolysis product.

EFFECT: enlarged assortment of water-oil emulsion stabilizers.

1 tbl, 17 ex

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