Method of obtaining of biologically active preparation (versions) and biologically active preparation

FIELD: medicine; chemistry.

SUBSTANCE: biologically active preparation represents a liquid allocated of biological raw materials a destruction of cellular structure processed by constant and low-frequency variable or impulsive field, from information structures of initial raw materials, with characteristic frequencies of spectra of excitation inherent in last and absorption in an ultra-violet and dark blue part of a spectrum of 190-440 nanometers, and also versions of way of its obtaining. The way by the first version includes raw materials freezing, thawing and fast sublimation at temperature 30-45°C with the subsequent condensation and condensate processing by the combined constant field in a range of magnetic induction 0.01-100 mT and a low-frequency variable or impulsive field in a range of a magnetic induction 0.01-100 mT then separate the firm rest with obtaining of a target product in the form of condensate. The way by the second version includes raw materials drying under vacuum at warming with simultaneous processing by the combined constant field in a range of a magnetic induction 0.01-100 mT, and low-frequency variable or impulsive field in a range of a magnetic induction 0.01-100 mT, condensation on walls of the closed volume which temperature support within dew-point temperature then separate the solid residue with obtaining of a target product in the form of condensate.

EFFECT: expansion of functionality, an exception of by-effects and contraindications, reduction of terms and reception doses, augmentation of a period of storage and expansion of raw-material base at the expense of use of vegetative and animal raw materials.

8 cl, 11 ex

The invention relates to organic chemistry, biotechnology and medicine as a fundamentally new biologically active structured products to normalize the physiological state of the organism of man and animals, in particular, for the prevention of pathologies and restore the functional activity of organs and tissues.

Known biologically active drug with antioxidant and immunomodulatory effects, containing vegetable raw materials, subjected to homogenization by degradation and hydrolysis and heating at a temperature of 65-75°With, mainly with a particle size of 5 to 45 μm on dry biomass. (RU # 2283124, publ. 2006.09.10).

The disadvantages of this drug is narrow functional treatment and prevention opportunities, limited preventive effect, a high incidence of side effects and a large amount of contraindications for persons sensitive to the drugs of marine origin, long term and large amount of dosage of the drug to produce meaningful results due to the low bioavailability of the drug, a small storage period up to 6 months.

Known biologically active agent with immunomodulatory property, based on the organic components of the cell membranes of homogenizing the authorized animal tissue, containing thermostable, low-molecular components of cell membranes, modified during embryogenesis and/or proliferatio, and/or differentiation of cells, and/or pathology, preceded and/or accompanied regeneration and/or repair tissue. (RU # 2041717, 1995).

The disadvantages of this drug is narrow functional treatment and prevention opportunities for a wide range of diseases, high incidence of side effects and a large amount of contraindications, long term and large amount of dosage of the drug to produce meaningful results due to the low bioavailability of the drug, small storage period.

Known biologically active agent based on the organic compounds of water-soluble drugs hydrolysis ingredients crushed animal tissue containing composition is hydrolysis stable, low-molecular components orthoplasty and glycocalix cells modified with the processes of embryogenesis and/or proliferation and/or differentiation of cells, and/or pathology, preceded and/or accompanied regeneration and/or repair tissue. The drug consists of modified components orthoplasty and glycocalix cells thermostable components. (RU # 20041715, 1995).

The disadvantages of this is Reparata are narrow functional treatment and prevention opportunities for a wide range of diseases, the high incidence of side effects and a large amount of contraindications, long term and large amount of dosage of the drug to obtain significant results, due to the low bioavailability of the drug, small storage period.

Known biologically active drug and the method of its production, including the location of the layer of raw materials in a closed volume, drying under vacuum while heating by injecting fluid through the layer of raw materials and the subsequent selection of the volatile components, and drying is performed under vacuum 250-380 mm Hg, heating of raw materials spend the contact method to 35-41°With, as the coolant using generated in the drying process vapors, and volatile components are selected by or on their walls closed volume, the temperature of which is maintained within the range of the dew point, when the condensate is a biologically active water. (RU # 2001622, 1993, prototype)

The disadvantages of these methods, and preparation are the narrowness of functional therapeutic and preventive capabilities of the final product in relation to a wide range of diseases, high incidence of side effects and a large amount of contraindications, long term and large amount of dosage of the drug to obtain significant results, due to the lack of bioavailability of the drug, not effektivnaya structure derived biologically active water complete loss of dry residue, the exclusive use of vegetable raw materials, small storage period.

The technical task of the present group of inventions so linked as to form a single inventive concept is to provide effective biologically active drugs and expanding Arsenal of bioactive agents and methods for their preparation. A single inventive concept of the present group of inventions is the creation of liquid and solid drugs from plants and animal tissues, which will find application in medicine, biotechnology and the food industry.

The technical result, provides solution to this problem is to increase functional treatment and prevention opportunities for a wide range of diseases, with the exception of side effects and contraindications, the reduction of the duration and the dosage of liquid and/or solid preparation to obtain meaningful results, increase retention, expanding the resource base through the use of plant and animal raw materials. This result is due to the high activity and bioavailability of the obtained liquid drug, little different from the typical clean water with the simultaneous action of ultra-low doses of biologically active substances, which increases the specific activity, but INM is taut side effects. This result is also due to the high activity and bioavailability of the obtained solid product. Virtually unlimited shelf life is determined by effective structure bifurcated hydrogen bonds in clusters weak aqueous solutions, providing long-term existence of water clathrates with prints functionally active structures of the source of biological raw materials in the preparation of biologically active water.

Summary of the invention in the first embodiment of the drug is that the biologically active agent is a selected from the original biological material by destruction of the cell structure of intercellular and intracellular transparent liquid, a biologically active water containing ions of CA²⁺, Na⁺, K⁺and Cl^-processed after condensation of weak combined constant and low-frequency alternating or pulsed field, with the formation of quatrano spatially backbone skeleton of the spirals associated water molecules in cavities which are connected to the water molecules coordinating communication and presented in a coherent state to the cluster information structure of the feedstock, with its last characteristic frequencies of the excitation spectra and pogloshena is in the ultraviolet and blue spectrum region 190-440 nm.

At the same time frame of clathrates consists of spirals, the structure of which is a carrier of local modifications, representing the physical-chemical imprints functionally active molecules from the group of bioflavonoids, vitamins, protease inhibitors, enzymes, resveratrol, etc. included in the original biological raw materials, clathrates contain information structure of the group: tryptophan, tyrosine, phenylalanine, and cytochromes. The drug has a smell, taste, yield, polarization and conductivity characteristic of the source of biological material. The drug has antibacterial properties and stand-off is determined by the structure bifurcated hydrogen bonds in clusters, weak water solutions.

Summary of the invention according to the second version of the drug is that the biologically active agent is a dry part, isolated from the source of biological materials and consisting of all organic compounds and inorganic ingredients, the nature of this type of raw material precipitated after isolation from biological raw material of the liquid portion in the liquid form of the drug in the first embodiment of the drug.

The drug has baked to a powder or plastilinovye mass consistency, contains fats, proteins, fiber, carbohydrates, oils, vitamins, to acitelli, pigments, bioflavonoids and stores, the smell, the taste, the polarization, the persistence of excitation spectra and absorption, as well as the conductivity of the source of biological materials, and is soluble in the liquid in the first embodiment of the drug and has a standoff.

Summary of the invention according to the first variant of the method is that the method of obtaining biologically active compounds involves placing a layer of raw materials in a closed volume, freezing at temperatures from minus 5°-50°C, defrost and fast sublimation at a temperature of 30-45°With subsequent condensation and condensate treatment of weak combined constant field in the range of the magnetic induction from 0.01 to 100 MT and a low frequency alternating or pulsed field in the range of the magnetic induction of 0.01-100 MT, when the condensate is a biologically active liquid product, and the solid residue is a solid biologically active agent.

Preferably receive liquid medication is placed in a glass bowl egg-shaped, glass containers ovate within 2-10 hours are stirring the liquid preparation clockwise with a magnetic stirrer, a raw material is placed in a closed volume in powdered form, with humidity, sufficient to swell, which is 10-2 hours.

Summary of the invention according to the second variant of the method is that the method of obtaining biologically active compounds involves placing a layer of raw materials in a closed volume, drying under vacuum while heating by injecting fluid through the layer of raw material with simultaneous processing of weak combined constant field in the range of the magnetic induction from 0.01 to 100 MT and a low frequency alternating or pulsed field in the range of the magnetic induction of 0.01-100 MT, followed by selection of volatile components or them on the walls of the enclosed volume, the temperature of which is maintained within the range of the dew point temperature, and the drying is carried out at a vacuum 150-550 mm Hg, heating of raw materials spend the contact method to 30-41°With, as the coolant using generated in the drying process of the pair, when the condensate is a biologically active liquid medication, and the solid residue is a solid biologically active agent.

These options group of inventions based on the fact that all living organisms on Earth in the middle are 70-90% water and 16% to 19% protein. Water, having been in the body, captures all structural and informational elements of living matter. Therefore, allocating water from any organism, we get its matrix in liquid form. This matrix according to its physical, helices them, biophysical, sensory, and other parameters is different from a simple mixture of water and organic compounds, obtained by squeezing. For example, in the fresh juice. To obtain water matrix it is necessary to separate the inside of the cell and extracellular water from the solid parts of the plant or animal. The effect of the drug is mediated through effects on the skeleton from spiral bound water cluster, which contains the minimum component of the feedstock. The same applies to the physical factors, the reinforcing effect with the decrease in their intensity at specific intervals power and doses.

Present in the structure of the drug imprints in the form of certain "defects" are capable of prolonged survival and transitions subsequent dilutions up to the state when there is no substance itself of the feedstock. This condition holds only if the water does not evaporate. The same applies to the physical factors, the reinforcing effect with the decrease in their intensity at specific intervals power and doses.

The emergence in non-equilibrium conditions specific clusters of nanometer-sized "latent" phase, or quatrano, which, according to classical theory, there should not be. They cannot be described by the terminology of the known States of matter, because I represent is a completely original, "ultra-low" and "ultra-fast" form of his existence, without macroblog. Quatrone precursor not only of crystalline embryos, embryos" minerals, but also, for example, known fullerenes, relatively recently discovered class of carbon compounds, structural units which are hollow clathrates. The report of the member-correspondent of the Russian Academy of Sciences Amirkhanova. Institute of Geology, Komi science centre, Syktyvkar ("Clusters latent phase (quatrani), their properties and role in the formation of crystalline and non-crystalline materials").

The method of obtaining biologically active compounds from plant material is realized in the first embodiment of the method as follows.

Before loading into the chamber of the reactor of the original animal or plant material is washed with running water, ground in a meat grinder or a knife to see 2-3 Then a layer of raw material is placed in a closed volume and freeze at the temperature from minus 10°-50°C. is then thawing and rapid sublimation at a temperature of 30-45°With subsequent condensation and condensate treatment of weak combined constant field in the range of the magnetic induction from 0.01 to 100 MT and a low frequency alternating or pulsed field in the range of the magnetic induction from 0.01 to 100 MT.

As a result, the condensate is a biological and an active liquid medication, which contains ions of CA²⁺, Na⁺, K⁺and Cl^-and quatrone spatially backbone skeleton of the spirals associated water molecules in cavities which are connected to the water molecules coordinating communication and presented in a coherent state to the cluster information structure of the feedstock, with its last characteristic frequencies of the excitation spectra and absorption in the ultraviolet and blue spectrum region 190-440 nm.

In the drawings 1, 2 and 3 presents the image patterns of quatrano the liquid preparation made with an electron microscope.

The residue is dry (solid) biologically active drug.

The method of obtaining biologically active compounds from plant or animal raw materials is implemented according to the second variant of the method as follows.

Before loading into the chamber of the reactor of the original plant material is washed with running water, ground in a meat grinder or a knife to see 2-3 Then a layer of raw material is placed in a closed volume in the tray camera response. Before switching can fill with water to 100 ml of water per 1 kg of body weight. (Depending on the water content in the sample and in each case the amount of water varies). The camera is drying under vacuum while heating by blowing those which of monocytes through the layer of raw material with simultaneous processing of weak combined constant (magnetic induction in the range from 0.01 to 100 MT) and low-frequency alternating or pulsed (magnetic induction in the range of 0.01-100 MT) field (EMF) with subsequent selection of volatile components. Drying is carried out at a pressure (under vacuum) 150-550 mm Hg, which is installed in the vacuum valve. The heated raw material spend the contact method to 30-41°using an electric heater. As the coolant using generated in the drying process vapors, and volatile components are selected by or on their walls closed volume, the temperature of which is maintained within the range of the dew point.

As a result, the condensate is a biologically active liquid preparation that contains ions of CA²⁺, Na⁺, K⁺and Cl^-and quatrone spatially backbone skeleton of the spirals associated water molecules in cavities which are connected to the water molecules coordinating communication and presented in a coherent state to the cluster information structure of the feedstock, with its last characteristic frequencies of the excitation spectra and absorption in the ultraviolet and blue spectrum region 190-440 nm.

The residue is dry (solid) biologically active drug.

Examples preparation preparation

Example 1

Placenta pigs washed with water, passed through a meat grinder. Layer 1-3 cm raw material was placed in lock UTY volume in the tray camera response. Before turning poured water at the rate of 30 ml of water per 1 kg of body weight. In the chamber were freezing at a temperature of minus 12°C. Thawing and rapid sublimation was carried out at a temperature of 30°C. Processing of condensate produced weak combined constant field with a magnetic induction of 0.02 MT and low-frequency alternating field with a magnetic induction of 0.01 MT. As a result, the condensate is a biologically active liquid medication, and the residue is dry (solid) biologically active drug.

The liquid drug is collected in a glass bowl egg-shaped and produced in 6 hours stirring clockwise (right) using a magnetic stirrer.

Example 2

Umbilical cord and placenta 3 calves washed and minced in a meat grinder. Then a layer of 0.5-1 cm materials were placed in a closed volume in the tray camera response. Before turning poured water from a rate of 60 ml of water per 1 kg of body weight. In the chamber were freezing at a temperature of minus 30°C. Thawing and rapid sublimation was carried out at a temperature of 37°C. Processing of condensate produced weak combined constant field with a magnetic induction of 50 MT and a low-frequency pulsed field with a magnetic induction of 50 MT. As a result, the condensate is a biologically active liquid medication, and the balance - su is th (solid) biologically active drug.

The liquid drug is collected in a glass bowl egg-shaped and produced in 4 hours stirring clockwise (right) using a magnetic stirrer.

Example 3

Beef liver washed with water, filtered through a meat grinder. Then a layer of raw material 2-3 cm were placed in a closed volume in the tray camera response. Before turning poured water at the rate of 15 ml of water per 1 kg of body weight. In the chamber were freezing at a temperature of minus 50°C. Thawing and rapid sublimation was carried out at a temperature of 45°C. Processing of condensate produced weak combined constant field with a magnetic induction of 100 MT and a low-frequency alternating field with a magnetic induction of 100 MT. As a result, the condensate is a biologically active liquid medication, and the residue is dry (solid) biologically active drug.

The liquid drug is collected in a glass bowl egg-shaped and produced in 10 hours stirring clockwise (right) using a magnetic stirrer.

Example 4

Fresh beef washed with water, passed through a meat grinder Then a layer of raw material 2-3 cm were placed in a closed volume in the tray camera response. Before turning on water is poured at the rate of 100 ml water per 1 kg of weight. In the chamber were dried under vacuum by heating with the blowing of Teflon is sites through the layer of raw material with simultaneous processing of weak combined constant 35 MT and the low-frequency variables 55 MT field with subsequent selection of volatile components. Drying was carried out at a pressure (under vacuum) 150 mm Hg, which was set in the vacuum valve. Heating of raw materials held by the contact method to 41°using an electric heater. The coolant used is formed in the drying process vapors, and volatile components were selected by or on their walls closed volume, the temperature of which is maintained within the dew point temperature. As a result, the condensate is a biologically active liquid medication, and the residue is dry (solid) biologically active drug.

Example 5

Blood pigs layer of 1.6 cm was placed in a closed volume in the tray camera response. In the chamber were dried under vacuum by heating by injecting fluid through the layer of raw material with simultaneous processing of weak combined constant 80 MT and the low-frequency variables 80 MT field with subsequent selection of volatile components. Drying was carried out at a pressure (under vacuum) 350 mm Hg, which was set in the vacuum valve. Heating of raw materials held by the contact method up to 40°using an electric heater. The coolant used is formed in the drying process vapors, and volatile components were selected or their Nastenko closed volume, the temperature maintained in the range of dew point temperature. As a result, the condensate is a biologically active liquid medication, and the residue is dry (solid) biologically active drug.

Example 6

Fruits carrots washed and minced in a meat grinder. Then a layer of 0.5-1 cm raw material is placed in a closed volume in the tray camera response. Before turning poured water from a rate of 60 ml of water per 1 kg of body weight. In the chamber were dried under vacuum by heating by injecting fluid through the layer of raw material with simultaneous processing of weak combined constant 20 MT and a low-frequency pulse 70 MT field with subsequent selection of volatile components. Drying was carried out at a pressure (under vacuum) 550 mm Hg, which was set in the vacuum valve. Heating of raw materials held by the contact method up to 45°using an electric heater. The coolant used is formed in the drying process vapors, and volatile components were selected by or on their walls closed volume, the temperature of which is maintained within the dew point of minus 75°to minus 70°C. the resulting condensate is a biologically active liquid medication, and the residue is dry (solid) biologically active drugs is at.

Example 7

Cloves garlic, washed and minced in a meat grinder. Then a layer of 1-1,5 cm raw material is placed in a closed volume in the tray camera response. Before turning poured water from the calculation of 55 ml of water per 1 kg of body weight. In the chamber were dried under vacuum by heating by injecting fluid through the layer of raw material with simultaneous processing of weak combined constant 70 MT of low-frequency pulsed 60 MT field with subsequent selection of volatile components. Drying was carried out at a pressure (under vacuum) 350 mm Hg, which was set in the vacuum valve. Heating of raw materials held by the contact method up to 30°using an electric heater. The coolant used is formed in the drying process vapors, and volatile components were selected by or on their walls closed volume, the temperature of which is maintained within the dew point temperature. As a result, the condensate is a biologically active liquid medication, and the residue is dry (solid) biologically active drug.

Example 8

Cabbage cabbage washed and minced in a meat grinder. Then a layer of 2.5-3 cm raw material is placed in a closed volume in the tray camera response. Before enabling filled with water at the rate of 25 ml of water per 1 kg of body weight. The camera was made you the suturing under vacuum while heating by injecting fluid through the layer of raw material with simultaneous processing of weak combined constant of 100 MT and a low-frequency alternating 95 MT field with subsequent selection of volatile components. Drying was carried out at a pressure (under vacuum) 225 mm Hg, which was set in the vacuum valve. Heating of raw materials held by the contact method to 37°using an electric heater. The coolant used is formed in the drying process vapors, and volatile components were selected by or on their walls closed volume, the temperature of which is maintained within the dew point temperature. As a result, the condensate is a biologically active liquid medication, and the residue is dry (solid) biologically active drug.

Example 9

Peeled from branches grapes washed and minced in a meat grinder. Then a layer of 1.5-1.8 cm raw material is placed in a closed volume in the tray camera response. In the chamber were dried under vacuum by heating by injecting fluid through the layer of raw material with simultaneous processing of weak combined constant 40 MT and a low-frequency pulse 40 MT field with subsequent selection of volatile components. Drying was carried out at a pressure (under vacuum) 150 mm Hg, which was set in the vacuum valve. Heating of raw materials held by the contact method to 34°using an electric heater. The coolant used obrazuyuschie is in the process of drying pairs, and the volatile components were selected by or on their walls closed volume, the temperature of which is maintained within the dew point temperature. As a result, the condensate is a biologically active liquid medication, and the residue is dry (solid) biologically active drug.

Example 10

The fruits of Jerusalem artichoke washed and minced in a meat grinder. Layer 1-3 cm raw material was placed in a closed volume in the tray camera response. Before turning poured water from the calculation of 95 ml of water per 1 kg of body weight. 10 hours in the tray was produced swelling of raw materials. In the chamber were freezing at a temperature of minus 40°C. Thawing and rapid sublimation was carried out at a temperature of 37°C. Processing of condensate produced weak combined constant field with a magnetic induction of 0.9 MT and low-frequency alternating field with a magnetic induction of 1.5 MT. As a result, the condensate is a biologically active liquid medication, and the residue is dry (solid) biologically active drug.

The liquid drug is collected in a glass bowl egg-shaped and produced in 3 hours stirring clockwise (right) using a magnetic stirrer.

Example 11

Get both versions of the method the liquid product contains water with ions of CA²⁺, Na⁺, K⁺and Cl^{-/sup> pre-processed weak combined constant (0.01 to 100 MT) and low-frequency variables (0.01 to 100 MT) field that causes fluorescence of albumin. The magnitude of the effect depends on the frequency of the alternating field and the combination of ions. Contains a fairly large (700-900 daltons) and stable molecular aggregates. The water component of the biological solution is the primary target of action of EMFs. Detected a significant change in the content of free oxygen in water and aqueous solutions of eletrolytes under the action of electromagnetic radiation of millimeter range. It is shown that water and dilute aqueous solutions of glycitein have the sensitivity to long-term effects of weak EMFs (UV). Along with induced during processing processes were also observed spontaneous long-term process lasting several days. The duration and complexity of the dynamics of transient processes allows to treat water and aqueous solutions as non-equilibrium systems as a result of implementation of the present processing method for self-organization and, consequently, sensitive to weak physical effects. It is proved, on the basis of modular generalization of crystallographic data, all possible types of algorithms of generating the hierarchical backbone structures linked to the ow of water, matching morphological patterns, most often found in nature. The idea of hierarchical modular structures of bound water reflect the potential of education on the basis of their spatial structures of biopolymers and biological systems. Instead of the conventional model of direct interaction of ligands with biological targets a model of their interaction by directed hydrogen bonds with the backbone skeleton of the spiral-bound water. The action of ultra-low doses of biologically active substances is also mediated through their impact on the frame of the spiral bound water.}

^{Liquid medication has expressed General structure and properties that do not belong to any known substance on Earth, and consists mainly of extracellular and intracellular water necessary structures. Moreover, the liquid drug is always perfectly transparent, sometimes with a slight odor of raw materials. However, most often they is almost no smell. The liquid preparation can be stored in sterile containers for many years (7-10 years, perhaps centuries) without preservatives and cooling, absolutely without changing the organoleptic and physical properties: transparency, smell, taste, yield, polarization, permanence of the spectra of the excitation and absorption, conductivity, etc. during storage the Oia spectral characteristics of the drug are not changed, what about the preservation of structures that define the basis of the spectrum. Liquid preparation consists of water and minor amounts of aromatic substances, amino acids, proteins, ions, and unidentified compounds. Extremely high biological and pharmacological activity of the liquid preparation. Liquid drug acts on the patient body, self-correcting structure of homeostasis.}

^{Each kind of living organism has its own individual set of substances. Therefore, each of them differs from other representatives of the living world. For example, garlic is a repository of allicin and crab chitin, grapes, resveratrol, etc. it is Known that resveratrol (resveratrol) is a natural compound that can increase the life expectancy posvonotchnik organisms type of yeast, worm, .elegans and fly Drosophila. The high content of resveratrol in the seeds and skins of black grapes.}

^{The right sugar and left amino acids of the living substance of the body is exposed to certain external chirarizumu factor. However, when exposed to chemicals tendency of these "parts" of living matter, one or the other "side"is manifested in different degrees. The effect depends on the dose, the chemical composition of the "agent", direction of rotation, and according to "sign" the effect of initial characteristics of the volume is the same. The result was the kinetic paradoxes - "stratification" of the properties of biologically active drug. Decreasing its concentration to the minimum possible practically without changes is active, but the side effects disappear. The same applies to the physical factors, the reinforcing effect with the decrease in their intensity at specific intervals power and doses.}

^{The approach to the kinetic explanation of the paradox is based on the idea of allosteric interactions in the structure of clathrates. Assume that the enzyme or receptor contains several centers with different affinity to the substrate, for example, the dissociation constant for one centre equal to 10-13M, and the other 10-8M When you enter a low dose of the drug, its molecules are mainly associated with the high performance centre. When the dose is increased activates the second center. He interacts with allosteric first center, lowering its affinity to the substrate, and then all the molecules that have been associated with the first center, "go" with him. Again to contact him they can only after the drug concentration approaches the value of the dissociation constants of the complex of the ligand with the first center reached under the influence of the second center.}

The structure of water in dilute solutions of liquid PR the drugs for a long time after their preparation is undergoing changes and only after a few days comes to a stable equilibrium. The dynamics of transient relaxation processes can be both monotonic and oscillatory. The structures of water and aqueous solutions can be considered as the primary target for small concentrations of dissolved substances, and the impact of weak fields. The corresponding change of the properties of water leads to changes in the properties of biomembranes, and hence to changes in the functional activity of the cells. For verification of clathrate model the liquid preparation methods were applied dielectric and differential scanning calorimetry. The first method is confirmed that vysokorazvetvlennyi solutions contain free and bound in the form of clathrates molecules. The second method allowed to determine that the phase transitions caused by the breaking of clathrates at a certain temperature, which should not be exceeded when receiving the drug. This temperature is limited by the specific clathrates, which are characteristic of the original products. Surrounding structural nanoparticles of biologically active substances, clathrates "imprint" of its structure, and these prints can live long enough. Based on the fact that water is a single structure, dissolution therein of certain substances leads to the appearance in this structure, certain "defects"that are capable of long smestow is and transitions subsequent dilutions up to the state, when there is no substance itself. ("Effect of ultra-low doses of biologically active substances and low-intensity physical factors" Abbatecola, And, Elimeliah) Internet. Based on our unique data, all indicates that "prints" structural nanoparticles and clathrates water can exist for many years unchanged. This condition holds only if the water does not evaporate, so there is no condensation of the liquid preparation.

Certain conclusions about the structure of liquid products and solutions were obtained from the study of luminescence in dilute solutions of liquid drug. The intensity of the luminescence depends on the retention time and the presence of an impurity having or not having its own luminescence. When spectrophotometry in the band 190-440 nm of excitation spectra and absorption in the UV and blue part of the spectrum, as a rule, there are one or two peaks that are characteristic only of this type of plant or animal. This phenomenon can be used for identification or certification of the liquid preparation obtained from any biological object as a marker of belonging to one or another type.

The results of spectrophotometry.

Identification clear liquid preparations was performed using the absorption spectra in the ultraviolet and blue the region (190-420 nm). The research was carried out on a Spectrophotometer U-2000 (Hitachi). In the spectral analysis revealed that each liquid medication has its absorption spectrum with a pronounced one or two absorption peaks. These peaks correspond to electronic transitions in molecules (obviously, proteins)that are in fluid investigational product, and may be the natural characteristics of liquid biological products. And along with them, always at the far end of the spectrum (1000 nm) has a band characteristic of the water molecules.

Absorption spectrum of the liquid preparation, for example, derived from beef liver, is located in the region of 280 nm and emission in the region of 420 nm. Absorption spectrum of the liquid preparation of beef is in the region of 280 nm and emission in the region of 420 nm. Absorption spectrum of liquid drug from the blood of pigs is in the area of 282 nm, and the emission is in the area of 423 nm. Absorption spectrum of the liquid preparation from rosemary has a maximum at 266 nm. Absorption spectrum of the liquid preparation from cabbage is a maximum at 236 nm. Absorption spectrum of the liquid preparation from the Jerusalem artichoke has a maximum at 208 nm and the maximum emission - 316 nm. Absorption spectrum of the liquid preparation from the wormwood has a maximum at 192 nm and the maximum emission of 257 nm. Absorption spectrum of the liquid preparation from the black grapes has maximum at 206 nm, and a maximum of issuing the - 277 nm.

For the treatment of various diseases of liquid and solid drugs can be used together or separately.

Solid product, usually dry "crust" mass in the form of a powder or plastilinovye mass. It depends on the rounded object and its type. For example, grapes - plastilinovyy, and garlic powder. Stored solid drug in the same way for years in non-sterile packaging. When mixing solid and liquid drugs even after many years, it turns out the composition, organoleptic characteristics which are manifested as a freshly-picked raw materials.

The dry solid product contains all the ingredients: cellulose, starch, proteins, fats, minerals, vitamins, bioflavonoids, pigments, dyes, source of raw materials. The latter is important for the food and cosmetic industry. The degree of dehydration of solid (dry) of the drug depends on the amount of water in the feedstock and the degree of binding with its solid part of a specific raw material. When splitting the grapes even at a high vacuum glucose and gluten do not allow to dry grapes to powder. His solid preparation is always plastilinovyy. Parsley turns into powder even at a small negative pressure in the reactor. The solid product of plant or animal origin is to be placed can be applied in the light, the food industry, cosmetics, biotechnology, medicine, etc.

Clinical examples of the use of liquid and dry products in medical practice.

Example 1

30 women aged above 60 years of daily put on the face mask of cream with liquid preparation obtained from the placenta of the pig. After 3 months in 70% flattened nasolabial fold, decreased swelling under the eyes. In the area of the corners of his eyes disappeared "crow's feet". The use of the drug for 6 months all women have led to effective facial rejuvenation.

Example 2

Obtained from umbilical cords and placentas calves liquid product was used as a preservative for storage of stem cells derived from bone marrow of these same calves.

Stem cells from these calves were kept in the preservative for 2 years at room temperature. Periodic monitoring (3 months) has allowed to establish that the stem cells are stored without change of structure and activity.

Example 3

Patient L. 59 years. The diagnosis of gastric Cancer 4 tbsp. MTS in the liver and peritoneum. Cachexia. 2 years ago was diagnostic laparotomy, with the imposition of bypass anastomosis. A full course of chemotherapy. Six months ago there were pains in the stomach and difficulty in the process of swallowing solid food. When complaints about constant is s pain in the epigastrium and right hypochondrium, dyspnea, ascites, stool retention. A state of moderate severity, low power, pale skin. In blood there is a decrease in the number of erythrocytes, hemoglobin, albumin, increased liver transaminases level. On computed tomography in the stomach is determined by the volumetric education 4x5 see In the right and left lobes of the liver are determined by several entities of different sizes with uneven edges. The patient was asked to take the liquid medication from celandine, 2 tablespoons in a glass of water 3 times a day.

After 12 days from the beginning of treatment the patient has completely disappeared pain and appetite. In the process of treatment of a tumor in the stomach decreased in size up to 0.5×0,7 see After 3 months the tumor in the stomach is not defined, liver metastases are rare. Blood and urine in norm. Weight restored. No complaints.

Example 4.

Patient C. for 60 years. Diagnosis: Chronic destructive bronchitis. Sick 12 years. Frequent antibiotic therapy and anti-inflammatory treatment. However, every year 2-3 times came aggravation. In recent years, increased shortness of breath and appeared acrocyanosis. At the time of treatment complained of severe weakness, persistent pain in the lungs, cough, shortness of breath. A serious condition, low power, pale skin with cyanotic shade. The blood was the fast decrease in the number of erythrocytes, hemoglobin, leukocytes, albumin, increased ALT and ACT. In the right lung are determined by several bands of different sizes. In the right pleural cavity free fluid (exudate). The patient was offered treatment liquid and solid preparations obtained from plantain. The course lasted 3 months. After 10 days from the beginning of treatment the patient has completely disappeared pain and appetite. On the third week of treatment decreased shortness of breath. During treatment, recovered blood picture. After 6 months, a marked gradual improvement in blood, directly associated with the medication of plantain.

Example 5.

Patient O. 49 years. Diagnosis: Systemic lupus erythematosus.

Ill for 13 years. 13 years ago years ago, accidentally discovered the increase of the cervical lymph nodes and redness of the skin. Was diagnosed with lupus on the basis of complaints and analyses. Were treated with hormones, antiinflamatory drugs and GE is sorbcia. The treatment results in the six months to reduce inflammation in the joints and erythema of the skin. The patient is offered intensive therapy drugs from the violet field and red in the ratio 1:1. The technique was carried out three times a day half a Cup of these drugs 30 minutes before meals. The course lasted 3.5 months. After 14 days of starting treatment, the patient had completely disappeared back pain and joint pain. On the fourth week of the treatment of skin erythema decreased. After 6 months of repeated course these same drugs. During therapy swelling of the joints and the pain was not renewed. In the blood are not circulating immunoglobulin complexes. ESR - 14 mm/h

Currently, no complaints.

Example 6.

Patient N. 49 years. The automotive technician is C: Cancer of the left breast. MTS in the ribs and liver.

Complaints of excruciating pain in the chest and shortness of breath.

Three years ago, accidentally discovered in the left mammary gland tumor formation 4×3 see After a puncture biopsy was diagnosed with infiltrative cancer, after which produced a total mastectomy on the left, the removal of axillary lymph nodes. After the operation conducted radiotherapy and chemotherapy. Two years ago in postoperative scar opened a small ulcer that within 2 months increased to 3×4 see chemotherapy and local treatment did not lead to the desired result. In the area of the left breast there is a plague 10×6×9 cm with purulent malodorous discharge. On computed tomography in the field of 7-8 ribs on the left is determined by the volumetric education 4×3 see In the left pleural cavity free fluid (exudate). In the right lobe of the liver metastasis 3×3×4 see the Patient of the proposed treatment liquid preparations of pedophila and sea Holly field in a 1:1 ratio. The technique was carried out 3 times a day, a teaspoon of each drug in 200 ml of water half an hour before meals. The wound overlap band of the liquid preparation made from onions. The course lasted 3 months. 19 days from start of treatment, the patient was completely gone pain in left chest and shortness of breath have decreased. On the third week the Les treatment, the wound was cleansed from pus, started epitheliopathy from the edges. Two months later healed completely. In the treatment of metastases in the region of the ribs decreased to 0.1×0,2×0,1 see After 3 months underwent a second course of drugs according to the same scheme. During treatment the wound epithelials, metastasis to the ribs was illnerova. During treatment with drugs was noted positive dynamics in the blood.