Agent for superficial mycoses treatment
SUBSTANCE: agent for superficial mycoses treatment contains antimycotic Ketoconazole; emulsion carrier including emulsifiers, castor oil, water; end additives stabiliser and preserving agent. Agent is aerosol liniment additionally containing antioxidant Dibunolum or butylhydroxytoluene. As emulsifier agent contains emulsifiers of the first and second type that are distilled monoglycerides, emulsion wax, Tvin 80; as stabiliser it contains oxymethylpropylcellulose (OMPC), and preserving agent is mixture of Nipaginum and Nipazole.
EFFECT: provided lasting contact medicinal substance with affected region and its environmental protection; lowered risk of mycosis transfer from infected person to other people and higher sedimentation stability of agent.
3 tbl, 4 ex
The present invention relates to the field of medicine, namely pharmaceuticals, and can be used in the pharmaceutical industry for the production of the drug used in the treatment of superficial mycoses.
Fungal infections are fairly common diseases. Of particular relevance to their treatment gains in connection with the rapid growth of immunodeficiency, as weakening of the immune protection activated many fungi, with which the body peacefully coexisted before. In addition, fungal infections can occur in cases of violation of the microbial flora of man.
In this regard, the important is the problem of finding new reliable and at the same time safe antifungal agents.
Well-known antifungal agents with broad spectrum of activity, representing a composition, containing a pharmacologically active agent, terbinafine (RF Patent 2093152 invention "ANTIMYCOTIC PHARMACEUTICAL COMPOSITION (OPTIONS)", CL AC 31/135, publ. 1997.10.20). Terbinafine is an effective antifungal agent in oral and local application. It is effective against a wide spectrum of fungal diseases. In Russia there are several dosage forms terbinafine hydrochloride for external use: - terbinafine (Lamisil) cream 1% (t the BA 15 g), Novartis Pharma, Switzerland; Activin (Exifine) cream 1% (dose 10 g), Dr. Reddy's Laboratories, India; terbinafine (Lamisil) is a topical solution 1%, vial, 30 ml, Novartis Pharma, Switzerland; terbinafine (Lamisil) spray for topical application of 1%, bottle of spray 30 ml, Novartis Pharma, Switzerland. Famous cream terbinafine 1% of the company Novartis Pharma, Switzerland, contains terbinafine hydrochloride 1%, benzyl alcohol, isopropyl myristate, monostearate, sorbitan, sodium hydroxide, Polysorbate 60, stearyl alcohol, cetyl alcohol, cetylpalmitate, demineralized water. (Reference VIDAL. Drugs in Russia, M., Attraversare, 2001, strb-305). However, the application of terbinafine may cause adverse reactions : headaches, gastrointestinal symptoms, anomalies detected during the study of the liver, dermatological symptoms such as itching, hives and rash, and taste disorders, such as loss of taste.
The most effective and low-toxic antifungal agents are clotrimazole and ketoconazole. On the basis of the known number of pharmaceutical compounds: ointments, creams, vaginal gels, emulsions, etc. Known composition in the form of a gel, containing (wt.%): as the active component - clotrimazole - 0.5-3; as a gelling agent carboxyvinyl polymer is 0.1-3; as solvent - 1,3-butyleneglycol - 5-90 and predpochtitel is about, additionally ethanol - 30; as the pH regulator is an organic amine and water [patent EP 0186055, IPC AC 31/415, 1986]. A disadvantage of the known compositions is that the solvent used is not physiologically indifferent. Alcohol has a defatting to the skin effect, leading to exacerbation of the pathological process, additional drying with the skin. In addition, the solvent does not provide prolonged contact of medicinal substances from the affected area, has tanning and irritant effect on skin and mucous membranes. Tanning action of ethyl alcohol leads to strengthening and thickening of the walls of the spores of the fungus, and thereby reduces therapeutic effect of the drug.
Known for a number of pharmaceutical antifungal compounds: ointments, creams, emulsions, etc., lipophilic-hydrophilic basis, providing a high drug release and not having irritating to the skin. Thus, in the Federal Republic of Germany patent N3243544 (IPC AC 31/415; AC 47/10; publ. 30.05.1984) proposed composition with clotrimazolum on lipophilic-hydrophilic base containing: clotrimazole - 10%, span (arbitarily ether fatty acids) - 2% (emulsifier), twins (polyoxyethylenesorbitan ether fatty acids) - 1.5 (emulsifier), synthetic spermaceti - 3% (lipophilic base), cetylstearyl alcohol - 3,5% (stabilizat is p), isopropylmyristate - 13,5% (lipophilic base), benzyl alcohol 1% (preservative), water up to 100% (hydrophilic base). Also known emulsion compositions suitable for use in the form of ointments, gels, creams. In the United Kingdom patent N2187956 (IPC AC 31/41; AC 31/415; AC 33/38; AC 31/41, publ. 23.09.1987) described ingredients: clotrimazole is 1%, a silver salt sulfapyridine - 1%, vaseline oil - 6%, cetosteatil alcohol was 7.2%, Atamanova of 1.8%, paraffin white - 15,0%, glycerin 5%, and water to 100%. In the Federal Republic of Germany patent N3600947 (IPC AC 31/415; AC 47/10; AC 47/26, publ. 16.07.1987) described emulsion and lipophilic-hydrophilic compounds clotrimazolum used in the form of a cream: clotrimazole - 1%, span 60 (arbitarily ether fatty acids) - 2%, the twin 60 - 1,5%, cetylpalmitate - 3%. Lannete (a mixture of primary hexadecenoic and octadecenoic alcohols) - 10%, ethylene glycol monotropy ether - 31,25%, water - 41,25%. The disadvantage of these compositions is the use of hydrocarbon bases (vaseline, vaseline oil, paraffin), which is slowly and incompletely release of medicinal substance, can cause disorders in the physiological functions of the skin, to provide alligatoroidea and sensitizing effect.
Closest to the proposed invention, the essential features and the achieved therapeutic effect is a pharmaceutical composition with p is olivegrove activity containing clotrimazole or ketoconazole; emulsion liofilizirovannuu basis, including castor oil and emulsifier, which contains a derivative of methylcellulose; target additive is a stabilizer and a preservative; sodium benzoate and succinic acid in the following ratios, wt.%: clotrimazole or ketoconazole is 0.5 - 10; derived methylcellulose - 1-5; castor oil - 8-20; sodium benzoate (0.01 to 7; succinic acid is 0.01-5; target additives up to 100 (patent RF №2120305 to the invention PHARMACEUTICAL COMPOSITION HAVING anti-fungal ACTIVITY, AND METHOD thereof", IPC AC 47/38, AK 47/44, AK 31/415, publ. 20.10.1998). Known pharmaceutical composition does not cause allergic and sensibilise action, does not form a solid film when dry. The presence of castor oil in the composition provides high bioavailability of the composition. The disadvantage of the pharmaceutical composition is a low sedimentation stability and lack of hygiene when using. Ointment form of known composition requires special conditions of use, can contaminate clothing and impede the natural breathing of the skin. In addition, the use of funds will not protect against infection with other people
The task to be solved by the invention is the creation of a new headlight is aseptically composition for the treatment of superficial mycoses, having a high therapeutic effect and improved characteristics that remain stable over a long time - at least two years.
The technical result that can be obtained by carrying out the claimed invention is:
- improving therapeutic effect by providing prolonged contact of medicinal substances from the affected area and protect it from external influences;
- reducing the risk of transmission of infections from an infected person to other people;
improving hygienic use;
- improved sedimentation stability.
This technical result is achieved in that the means for the treatment of superficial mycoses contains antifungal substance - namely ketoconazole; emulsion media, including emulsifiers, castor oil, water, additives target stabilizer and preservative. The tool is made in the form of aerosol liniment, further comprises an antioxidant, which used BHT or equivalent. As emulsifier declare that the product contains emulsifiers first or second kind, distilled monoglycerides, emulsion wax, tween 80; as a stabilizer contains oxymethylphenyl (OPMC), and as a preservative mixture, SOS is ojasuu of nipagina and nipazola, when the following ratios, wt%:
|Of distilled monoglycerides||0,5-5,0|
|Dibunola or butylhydroxytoluene||0,01-0,5|
|Purified water||to 100 g|
In all cases, implementation of the proposed remedy for the treatment of superficial mycosis is different from the above known closest to him means:
- implementation in the form of aerosol liniment;
- additional content of antioxidant, which used BHT or butylhydroxytoluene;
- content as an emulsifier emulsifier of the first and second kind - distilled monoglycerides, emulsion wax, tween 80;
- content oxymethylphenyl (OPMC) as a stabilizer;
- content of the mixture consisting of nipagina and nipazola, as a preservative
- the following ratio of components, wt.%:
Ketoconazole 1,0-5,0 Of distilled monoglycerides 0,5-5,0 Emulsion wax 0,1-1,0 Castor oil 1,0-10,0 Tween-80 0,5-5,0 Dibunola or butylhydroxytoluene 0,01-0,5 Nipagina 0,05-0,5 Nipazola of 0.01-0.1 OPMC 0,5-5,0 Purified water to 100 g
The proposed ratio of operating, auxiliary substances and bases is optimal in terms of quality and quantity, found experimentally, is the original, unknown in the prior art.
The proposed composition is an aerosol liniment used in the form of a spray, dosed by a pump and spray push button type, which has high bioavailability; do not dry the skin; this does not affect the natural breathing of XI; washes off easily with water and will not stain clothes; has a prolonged action; hygienic to use.
Ketoconazole is an antifungal drug from the group of imidazole derivatives. Important features of ketoconazole are its effectiveness when administered, as well as its impact spacecraft is on the surface, and systemic mycoses. Linked to the drug-impaired biosynthesis of ergosterol, triglycerides, and phospholipids necessary for the formation of cell mambrane mushrooms. Assign ketoconazole in superficial and systemic mycoses: dermatomycosis and onychomycosis caused by blastomycete; avium scalp; vaginal mycosis; blastomycetes the oral cavity and gastrointestinal tract, genitourinary tract and other fungal infections of internal organs.
The preservatives used in the proposed structure can be represented by the following components: mixture of nipagin and nipazola. The introduction of the preservative in the composition ensures the stability of the drug in relation to antimicrobial action. Nipagin inhibits the growth of fungi; nipazol yeast. These preservatives are used, for example, FS No. 42-1460-89; FS No. 42-2079-91.
As an antioxidant in the proposed composition is used equivalent, which is used as an antioxidant in medicines for external use. As a stabilizer of the dispersed phase (ketoconazole), the proposed composition is used oxymethylphenyl (OPMC)providing sedimentation stability. For suspension must have a uniform distribution of the dispersed phase in the liquid with the food when drinking (GF XI) - resuspendable maintained throughout the shelf life. The results of the evaluation of the sedimentation stability are presented in table 1.
Comparative evaluation of the effect of the dispersion medium on the sedimentation stability of suspensions of ketoconazole (n=5)
|The dispersion medium||Shelf life|
|1 day||2 days||5 days||1 month||12 months||24 months||36 months|
|Emulsion without OPMC||Uniform distribution on volume||Resuspendable satisfy your high.||Not resuspended||Not resuspended||Not resuspended||Not resuspended||Not resuspended|
|An aqueous solution OPMC without emulsion||Uniform distribution on volume||Uniform distribution on volume||Resuspendable satisfy your high.||Not resuspended||Not resuspended||Not resuspended||Not resuspended|
|Emulsion with the addition of OPMC||Uniform distribution on the volume of the mu||Uniform distribution on volume||Uniform distribution on volume||Resuspendable with saved him a uniform distribution for 5 days||Resuspendable preserving a uniform distribution for 5 days||Resuspendable preserving a uniform distribution for 5 days||Resuspendable preserving a uniform distribution for 5 days|
In addition, UPMC forms on the skin is thin, breathable film, which after being committed on the skin, provides long contact of medicinal substances from the affected area, protects it from external influences and reduces the likelihood of transmission of infections to other people. Oxymethylphenyl used, for example, on THE 2231-107-0574-2755-96.
As the carrier in the proposed composition is used, the emulsion composition which can be represented by a mixture of emulsifiers, dispersion medium and dispersed phase (castor oil).
As emulsifiers can be used emulsifiers of the first and second kind, for example, distilled monoglycerides on THE 10-1197-95; emulsion wax on THE 915411-012-20820231-01 and tween-80 (imported), which are widely used in pharmaceutical and cosmetic production quality is ve emulsifying components.
As the dispersion medium use water purified by FC 42-2619-97.
As the dispersed phase in the emulsion is used castor oil with penetrating power due to ricinoleic acids. The presence in the composition of castor oil provides high bioavailability of the proposed structure. In addition, the oil softens dry skin, which is especially important when hyperkeratosis treatment.
The proposed set of media components provides a uniform dosing and stability of the dosage form.
When the concentration of ketoconazole in the composition is less than 1% of the drug is pharmacologically ineffective concentration of more than 5% technologically feasible.
The concentration of castor oil (from 1 to 10%) due to the pharmacological efficiency and expediency.
Concentration OPMS (from 0.5 to 5.0%) due to technological efficiency. Less than 0.5%, it does not ensure the stability of the dispersed phase, more than 5% over the long-term storage in the system is the formation that is accompanied by the impossibility of dispensing.
The proposed ratio of preservatives in the composition provides for the preservation of biological activity and the absence of contamination of the medicinal product putrefactive bacteria and fungi during storage, as well as maintain the pH level.
When reducing the number of any component creates an opportunity for the growth of pathogenic organisms, if the increase is not excluded allergic reactions when using the composition.
Antioxidant exerts its action with concentrations of 0.05%, its content in the composition of more than 0.5% is inappropriate.
Examples of the composition of the proposed pharmaceutical composition is shown below.
|Of distilled monoglycerides||1.2 g|
|Emulsion wax (import)||1.0 g|
|Castor oil FS 42-1906-95||5.0 g|
|Tween-80 (import)||2.0 g|
|Dibunola VFS 42-457-75||0.1 g|
|Nipagina FS 42-1460-89||0,15|
|Nipazola THE 18-16-387-81||0,05|
|OPMC (TU 2231-107-0574-2755-96 or imported)||1.6 g|
|Purified water (FS 42-2619-97)||to 100 g|
|Of distilled monoglycerides||0,5|
|Dibunola or botiller is kitawala||0,01|
|Purified water||to 100 g|
|Of distilled monoglycerides||5,0|
|Dibunola or butylhydroxytoluene||0,5|
|Purified water||to 100 g|
|Of distilled monoglycerides||2,5|
|Dibunola or butylhydroxytoluene||0,25|
|to 100 g|
Examples with specified ratios of ingredients provide a pharmaceutical composition that meets the requirements of the global Fund XI (in appearance, microbiological purity and other parameters), stable during storage. Offered as an invention composition is an effective drug in superficial mycoses: dermatomycosis and onychomycosis caused by blastomycete.
Preclinical study of the effect of spray with ketoconazole was conducted on the basis of the clinical laboratory. Antimicrobial activity of the claimed medicinal preparation was studied in accordance with the "Manual on experimental (preclinical) study of new pharmacological substances" (2005).
Test the fungicidal activity of the dosage form was evaluated in relation to cultures of fungi in the yeast and mycelial forms. As the test microorganisms used strains of Candida albicans and Aspergillus niger recommended GF-X1 for testing antimicrobial activity. The cultures were grown on solid medium Saburo. From yeast cells were preparing the suspension according to the turbidity standard of 10 U and diluted in sterile saline to a concentration of 107cells/ml Conidia of Aspergillus niger suspended in a solution of Tween-80, 0.5% and counting the number of PR is led in the camera Goryaeva. The content of conidia was 108/ml.
The determination of minimum fungicidal activity was performed by the method of serial dilutions in liquid nutrient medium Saburo in increments of 2 times. The initial concentration of the test dosage form of the drug was 1 mg/ml in terms of substance. The test drug was compared with a known drug, selected as a prototype. After dilution in all test tubes number made the same microbial load in a volume of 0.1 ml, incubated for 24 hours at a temperature of 24°C. In connection with the fact that the mixture of the drug with a nutrient medium are not transparent, the sowing was made from all tubes. To check the viability of used agar medium Saburo, seeding bar method. After incubation for 48 hours fungicidal effect was assessed by the absence of crop growth. Control was the growth of the test strain in a nutrient medium without drug. The results are presented in tables 2 and 3.
Fungicidal activity against Aspergillus niger
|Medication||The content of active substance, mg/ml|
|"+" is growth; "-" - no growth.|
The data show that the fungicidal action of the test drug is at a concentration of 63 mg/ml, and prototype completely inhibited the mycelial growth of the fungus when the content of 125 µg/ml Thus, the activity of the test drug against Aspergillus niger in 2 times higher than that of the prototype.
Fungicidal activity against Candida albicans
|Medication||The content of active substance, mg/ml|
|"+" is growth; "-" - no growth.|
For ease of presentation, results of concentration in the first 8 tubes of the row, where there is a complete inactivation of yeast cells, indicated as a range. The results show that the cells of the yeast Candida much more sensitive to the action of the substance in the checked dosage form than filamentous fungus. For the manifestation of fungicide enough of 0.2 µg/ml of the active substance in the test preparation and the prototype comparable.
The remedy for the treatment of superficial mycoses, containing antifungal substance - namely, ketoconazole, emulsion media, including castor oil, emulsifiers and water, additives target stabilizer and preservative, characterized in that it is made in the form of aerosol liniment, further comprises an antioxidant, which used BHT or equivalent, as an emulsifier contains emulsifiers of the first and second kind, distilled monoglycerides, emulsion wax, tween 80 as a stabilizer contains oxymethylphenyl (OPMC), and the preservative is a mixture consisting of nip is Gina and nipazola in the following proportions, wt.%:
|Of distilled monoglycerides||0,5-5,0|
|Emulsion wax||0,1 -1,0|
|Dibunola or butylhydroxytoluene||0,01-0,5|
|Purified water||to 100 g|
FIELD: medicine; pharmaceutics.
SUBSTANCE: pharmaceutical formulations contains as reactants 1) betamethasone dipropionate or 2) betamethasone dipropionate combined with gentamicin sulphate or 3) betamethasone dipropionate combined with gentamicin sulphate and clotrimazole. Pharmaceutical formulations contain ointment hydrophobic base containing white petrolatum and target additives - preserving agent and hydrophobic nonaqueous solvent. New pharmaceutical formulations have high antibacterial preserving action, high microbiological purity value and potentised antibacterial specific action showed by produced therapeutic effect and simultaneous prevention of by-effect local complication caused by activation of bacterial and mycotic infections.
EFFECT: prevention of by-effect local complication caused by activation of bacterial and mycotic infections.
18 cl, 11 tbl, 6 ex
FIELD: medicine, gynecology, pharmacy.
SUBSTANCE: invention relates to preparing vaginal suppositories containing laevomycetin and metronidazol as antibacterial components, pernisolon as an anti-inflammatory component, and cacao butter and lanolin as filling agents wherein these components are taken in the following ratio, g: laevomycetin, 0.25 ± 0.01; metronidazol, 0.25 ± 0.01; nystatin, 0.0625 ± 0.0025, or 250000 ± 10000 U; prednisolone, 0.003; cacao butter, 2 ± 0.2, and lanolin 0.05. Proposed suppository possesses the enhanced effectiveness against mixed infections of lower regions of female reproductive sphere and providing increasing remission period of disease.
EFFECT: improved and valuable medicinal properties of suppository, enhanced effectiveness of treatment.
1 tbl, 4 ex
FIELD: chemical-pharmaceutical industry, biochemistry, chemistry of peptides.
SUBSTANCE: invention relates to biologically active latarcin peptides possessing antimicrobial effect that can be used in biotechnology and medicine. Method for preparing antimicrobial latarcin peptides with reduced hemolytic activity by chemical or biotechnological method involves preliminary modeling the space structure of the parent latarcin peptide with high hemolytic activity in its α-helical configuration and change of hydrophobic amino acid residues for hydrophilic residue in the synthesis process when hydrophobic amino acids Ile7 and Phe10 are replaced for hydrophilic amino acids Gln7 and Lys10. After modeling the space structure of the parent latarcin peptide in its α-helical configuration measurement of hydrophobic properties of peptide is carried out additionally and with taking into account the molecular hydrophobic potential value in each point of peptide surface and detection of hydrophobic clusters and amino acid residues localized in them followed by identification of the most effective hydrophilic amino acid residues for using in the synthesis process. Method provides preparing peptides with significantly reduced hemolytic properties and to decrease cytotoxicity of latarcin peptides.
EFFECT: improved preparing method of peptides.
3 tbl, 2 dwg, 6 ex
SUBSTANCE: invention relates to a preparation used in treatment of mycosis and suppurative infections in animals and humans. The preparation represents propolis 10% alcoholic tincture containing additionally ichthyol in the concentration 8-12% of solution volume. Composition consisting of propolis alcoholic extract and ichthyol is used topically with break for 0.5-1 day. Invention provides enhancing effectiveness in treatment of mycosis complicated by suppurative infection and to reduce treatment period.
EFFECT: enhanced and valuable medicinal properties of preparation.
4 tbl, 2 ex
FIELD: medicine, pharmacy.
SUBSTANCE: invention relates to medicinal agents, in particular, to a drug showing antifungal effect prepared as ointment formulation and based on terbinafine hydrochloride. Invention proposes agent possessing antifungal effect and comprising terbinafine hydrochloride, rare-cross-linked polyacrylic acid, nipagin, Tween-80, vaselin oil, propylene glycol, sodium hydroxide and purified water wherein these components are taken in the definite ratio. Also, invention relates to a method for preparing this antifungal agent. Invention provides the high therapeutic effect of agent, increasing storage time, high stability against oxidation, high tropicity to skin and low cost.
EFFECT: improved and valuable medicinal and pharmaceutical properties of agent.
5 cl, 14 tbl, 3 ex
FIELD: medicine, mycology, dermatology.
SUBSTANCE: it is necessary to remove the affected part of a nail due to applying the equipment for apparatus chiropody. First, it is important to treat the protruded part of a nail with chiropody cutting pliers, then with a large metal drill with a transverse notch being a constituent of a chiropody set. One should scrape off the main hyperkeratotic masses, moreover, the thickness of nail reduced by one half. One should take a small metallic cutting with oblique transverse grooves out of chiropody apparatus set to remove hyperkeratotic masses up to a nail bed along the edge of a nail's healthy part. After that, with the help of a ceramic polisher one should polish the rest healthy part of a nail and the whole nail bed. After polishing one should treat a nail with antiseptic solution and apply a lacquer being of antimycotic action. The mentioned apparatus treatment should be fulfilled once monthly till the growth of healthy nails. Simultaneously, it is necessary to prescribe "Orungal" according to pulse therapy technique per 400 mg/d for 7 d followed by a 3-wk-long interval. At treating onychomycoses of feet one should apply about 3-4 course, that of wrists - 2 courses of pulse therapy. For softening nails one should apply a softening agent of "Gewol" firm (FRG). As lacquer being of antimycotic action one should apply "Loceryl". The innovation excludes traumatism of a nail bed, excludes hemorrhage and shortens terms of therapy.
EFFECT: higher efficiency of therapy.
FIELD: medicine, chemico-pharmaceutical industry.
SUBSTANCE: the present innovation deals with the plaster for the prophylaxis and/or the treatment of the dysfunction or the lesion of nail structure, especially, onychomycosis, ingrown nails, nail psoriasis, melanonychia of striate bodies and onychodystrophy, with application of the plaster mentioned and the ways of prophylaxis and/or treating the dysfunction and/or the lesion of nail structure due to application of the plaster mentioned. The latter is characterized by favorable therapeutic action onto the mentioned dysfunction or the lesion of nail structure, moreover, there is no necessity in drilling the foramen in the nail and/or in daily peeling the nail. The preferable variants deal with the plasters that contain hermetic protective layer and the layer fixed to the mentioned protective layer, moreover, the layer is in close contact with the nail and not obligatory with adjacent skin parts. The layer is made of the adhesive, the intensifier to penetrate skin and/or nail layer, therapeutic efficient quantity of sertaconasol as a free foundation and appropriate additives and/or additional pharmaceutically active agents.
EFFECT: higher efficiency of prophylaxis and therapy.
36 cl, 4 dwg, 6 ex
FIELD: chemical-and-pharmaceutical industry, biotechnology, medicine, in particular biologically active peptides with anti-microbial action.
SUBSTANCE: invention relates to peptides of general formula 1 (X1-Lys-Leu-X2-X3-X4-X5-X6-Arg-Lys-Ala-Ile-Ser-Tyr-Ala-Val-Lys-Ala-X2 (1) obtained by modification of starting structure of latarcin peptide, wherein X1 is absent or is glycine residue (Gly), or Phe-Gly dipeptide, or Leu-Phe-Gly tripeptide, or Gly-Leu-Phe-Gly tetrapeptide; X2 is glutamine residue (Gln) or isoleucyne residue (Ile); X3 is arginine residue (Arg) or lysine residue (Lys); X4 is arginine residue (Arg) or lysine (Lys) residue; X5 is lysine residue (Lys) or phenylalanine residue (Phe); X6 is glycine residue (Gly) or leucine residue (Leu); X7 is absent or is arginine residue (Arg), or Arg-Gly dipeptide, or Arg-Gly-Lys tripeptide, or Arg-Gly-Lys-His tetrapeptide, or Arg-Gly-Lys-Asn tetrapeptide.
EFFECT: new peptide with anti-microbial activity.
7 ex, 4 tbl, 3 dwg
SUBSTANCE: the present innovation deals with applying water-free warming lubricating compositions that contain multi-atomic alcohols, a gelatinization-inducing agent, a pH-regulating agent for treating fungous and bacterial infections and, also, with the methods to apply such compositions for warming, lubricating, introducing active ingredients and either preventing or treating fungous and bacterial infections. The innovation suggests nontoxic and nonirritating compositions being efficient for treating both fungous and bacterial infections.
EFFECT: higher efficiency of therapy.
10 cl, 11 dwg, 10 ex, 3 tbl
FIELD: medicine, dermatology.
SUBSTANCE: invention relates to using nitrogen oxide-producing components used for manufacturing a medicinal agent used in treatment or prophylaxis of subnail infection. Components comprise nitrite and organic acid. Before using each of indicated components is placed separately of one another. Nitrite is chosen from alkaline metal nitrites and earth-alkaline metal nitrites. Acid is chosen from formic acid, malic acid, maleic acid, acetic acid, lactic acid, citric acid, benzoic acid, tartaric acid, salicylic acid, ascorbic acid and their mixtures. At applying of indicated components on infected nail formed nitrogen oxides are able to penetrate through nail in the therapeutically effective dose. The effectiveness treatment index is similar or better as compared with oral treatment method but it shows lesser limitations with respect to safety including hepatotoxicity, severe cutaneous responses and interactions with other drugs.
EFFECT: improved method of treatment.
33 cl, 13 dwg, 16 tbl, 15 ex
SUBSTANCE: invention concerns medicine, namely to pharmacotherapy and can be used for prevention and treatment of freezing injuries. The composition from vegetative raw materials containing grass of bloodwort, flowers of wild camomile, common St. John's wort, birch leaves, door-weed, roots of common licorice, fruits of cinnamon rose (in the ratio 2:1:2:1:1:1:1), inserted in the form of 10% infusion to adult nondescript male rats promotes normalisation of level of the creatinine, inherent to cryo injury.
EFFECT: normalisation of level of the creatinine, inherent to cryo injury.
2 ex, 1 dwg
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention concerns the pharmaceutical, food and cosmetic industry. Perform extraction of the raw material crushed with water with obtaining of a water extract and pulp. The pulp, obtained after extraction of raw materials by the water, is extracted in two steps with ethyl alcohol. At the first step of extraction the constrictor is extracted within 4-6 hours using the following proportion: ethyl alcohol 1:(5-7) with concentration of alcohol of 30% either 50%, or 70%, at temperature of 60-75°C, obtaining an ethanolic extract of the first step of extraction and pulp; then, this pulp is repeatedly extracted within 4-6 hours with ethyl alcohol using the following proportion: ethyl alcohol 1:(3-5) with concentration of alcohol of 30% either 50%, or 70%, at temperature of 60-75°C, obtaining an ethanolic extract of the second step of extraction and pulp. After that the ethanolic extract of the first step of extraction is aggregated with the ethanolic extract of the second step of extraction.
EFFECT: increase in active substances content in an extract.
1 ex, 1 tbl
FIELD: chemistry; medicine.
SUBSTANCE: invention concerns carboxylic acid compounds of the formula (I) , where R1 is hydrogen or C1-4alkyl; m is 2 or 3; n is 0-2; R2 is phenyl, naphtyl, benzofuran, benzothiophene; Q is -CH2-O-Cyc1, -CH2-Cyc2, -L-Cyc3, each of R3a and R3b radicals is independently a hydrogen, alkyl, or the R3a and R3b radicals together form tetrahydro-2H-pyran; pharmaceutically acceptable salts, method of obtaining them, and pharmaceutical agent including this compound as active component. Compound of the formula (I) displays antagonistic effect on PGE2 receptor, especially EP3 receptor, which is its subtype, and is applied in prevention and/or treatment of itching, pain, urination disorder or diseases caused by stress.
EFFECT: increased efficiency of compounds.
11 cl, 37 ex, 5 dwg
SUBSTANCE: invention is referred to medicine, namely to dermatology, and can be used for treatment of hypertrophic or keloid skin cicatrixes. For this purpose inject diprospan in a cicatrix depth three times within 4 weeks from calculation of 7 mg on 5 cm2, but not more than 14 mg on all cicatrix at a time. After obtained medicamental oligotrophy, a cicatrical tissue is singly exposed to radiation of infra-red laser with wavelength of 540 nanometers, energy of impulse 50-150 mJ, pulse frequency of 1-4 Hz, duration of impulse - 1-2 nanoseconds. The method provides obtaining of cosmetic effect due to reduction of the area of cicatrix, formation of normotrophic cicatrical tissue; it is painless and safe, and does not affect surrounding tissues due to super-short interval of time of laser influence.
EFFECT: painless and hardly noticeable cicatrix.
1 ex, 2 tbl, 3 dwg
FIELD: medicine; surgery.
SUBSTANCE: method involves injections, in area of Achilles tendon, of a medicinal admixture consisting of 4 ml of 10% solution of anecaine, 25 mg of chymopsine, 80 mg of actovegin, 0.5 ml of neostigmine methylsulfate. The medicinal admixture is injected in periphery of Achilles tendon into projections of peripheric nerves n.tibialis and n.suralis on anticnemion.
EFFECT: intensifying local restorative processes and reduction of for time treatment.
FIELD: medicine, surgery.
SUBSTANCE: invention can be applied to septic wounds, cavities, and fistulas sanitation. The means comprise aqueous antiseptic solution, including 2.7-3.3% hydrogen peroxide, 0.9-10.0% sodium chloride, and carbon dioxide giving positive pressure 0.2 atm at +8°C.
EFFECT: invention provides safe and effective sanitation conditions, facilitates biological masses elimination, renders expressed geyser-like, dehydrating and antiseptic action.
SUBSTANCE: in case of extreme affections or pathological conditions accompanied by stem cells mobilisation, or in case of exposure of stem cell functional activity modifiers causing stem cells mobilisation, hyaluronidase is injected intraperitoneally in dose 500-1500 CU/kg within 2 days once a day.
EFFECT: increased level of stem cells mobilisation.
11 ex, 11 tbl
SUBSTANCE: general diabetes-corrigent therapy and low-intensity laser radiation are performed. Surgical processing of purulent-necrotic centre, hydromechanical wound clearing, ozone-therapy are followed with magnetic-infrared-laser radiation initially of trophic ulcer area within 2 minutes, then - of left infraclavicular region and inguinal vascular beams within 2 mines per zone, of thymus gland projection area within 1 mines, of pancreas projection area with scanning within 2 minutes. Frequency is 50 Hz. Infra-red radiation power of light-emitting diodes is 60 mW. Procedures are performed every second day, totally 10 - 16 procedures.
EFFECT: decreased dosage of sugar-lowering medicinal agents; reduced terms of wound surface clearance from purulent-necrotic tissues; pain syndrome reduction; inflammatory processes reduction; activated regenerative process; reduction of operative intervention volume and restoration of limb support function.
7 dwg, 1 tbl, 2 ex
SUBSTANCE: medicinal product contains carrier and dry mineral medicinal agent, particles of which are homogeneously distributed within volume and/or on the carrier surface. As dry mineral medicinal agent it contains powder with particles sized not more than 10 mcm of sterile mechanochemical activated natural zeolite with saline addition in ratio not less than 5:1. As saline addition product contains marine or lacustrine salt, or bischofite, and as natural zeolite product contains clinoptillolyte or chabazite, or heulandites, or phillipsyte. As carrier product contains material made as hydrophilic absorber and gas-permeable material of woven or non-woven material containing natural filaments or fibres. Carrier can be designed as bandage, or tissue, or plaster and is furnished with fixing elements on the surface. Carrier can be designed as capacity with netting dispenser in filler and cover. Content of zeolite powder and mixed zeolite with saline addition on carrier as bandage, tissue, or plaster does not exceed amount enough to form particle monolayer on one side of specified carrier.
EFFECT: high haemostatic, wound healing and anti-inflammatory properties of product.
7 cl, 1 tbl, 3 dwg, 4 ex
FIELD: medicine; pharmacology.
SUBSTANCE: invention concerns medicinal agent applied for various dermal diseases, injuries, stab, cut and septic wounds, burns and frostbites. Stated medicinal agent is antiseptic ointment containing iodophor as reactant, and polyethylene glycol as ointment base. As iodophor stated ointment contains pharmaiodine, and as ointment base - mixed polyethylene oxide-1500, polyethylene oxide-400 and glycerine taken in proportions as follows, (mass.%): pharmaiodine 5.0-20.0 (0.5-2.0 by active iodine); polyethylene oxide -1500 35-40; glycerine 15-17; polyethylene oxide-400 and other components to 100.
EFFECT: ointment is characterised by antiseptic, anti-inflammatory and wound healing action.
SUBSTANCE: invention concerns biopharmacology and medicine area. The antitumor agent representing a immunoliposome biological structure, including a liposome containing the therapeutic agent, sewed with a vector of peptide nature, thus for treatment of CNS tumors is declared, the liposome contains the therapeutic agent in a water phase, as a vector contains monoclonal antibodies to CD34+, and a linking represents 2-iminotiolan (IT) in 0.1% concentration. As a therapeutic agent, immunoliposome contains the substance chosen from the group: Daunomycin, Carminomycinum, Melphtalan, Methotrexatum, Cytarabinum, Doxorubicinum, Ricine. The method of obtaining of an antitumoral agent and way of inhibition of a tumor of the brain, consisting in agent administering due to item 1 in a pharmaceutically suitable carrier in effective quantity is declared also. Thus preliminary administer parenterally a preparation of hematological stem cells CD34+.
EFFECT: provision of highly effective delivery of an antitumor agent to a CNS tumor.
4 cl, 7 ex, 4 dwg