Method of biological age normalisation for persons with metabolic syndrome
FIELD: medicine; cardiology.
SUBSTANCE: method involves long-term individual hypocalorific diet, sensibly graded static and dynamic physical activity, administration of metformin by 500 mg twice per day after meals, essentiale forte by 1 capsule three times per day, tocopherol acetate by 0.1 g twice per day, and activated coal by 1 pill per 10 kg of weight by night.
EFFECT: bringing biological and chronological age of patients with metabolic syndrome.
2 ex, 1 dwg
The invention relates to medicine, namely to gerontology, cardiology and endocrinology.
There is a method of reducing biological age in patients with metabolic syndrome (MS) is the patent for the invention №2259196, however, this method can normalize the pathologically increased biological age for a long its application - not less than six months. This method is intended to normalize the pathologically increased biological age in patients with MS for two months, keeping him at the chronological level during the whole period of application of the proposed method.
The aim of the invention is the reduction of pathologically increased biological age of the patients with metabolic syndrome.
The essence of the proposed method lies in the fact that to reduce pathologically increased biological age patients with metabolic syndrome for a long period of time is assigned to a low-calorie diet, measured static and dynamic exercise, the drug Metformin at a dose of 500 mg 2 times a day after meals, Essentiale Forte 1 caps. 3 times a day, tocopherol acetate 0.1 g 1 caps. 2 times a day and as the sorbent is activated carbon 1 tab. on 10 kg of weight before going to sleep, not earlier than 3 hours after the last meal and other drugs.
The way you lead is in compliance with the biological and chronological age in patients with MS within two months. Subject to the subsequent recommendations of the proposed method may reduce the biological age below chronological, which will significantly improve the quality of life of patients with MS, to reduce their pathological burden, reduce the number of cases of temporary disability, to accelerate and improve the quality of patient treatment, to exclude disability, as well as extend the life and reduce mortality of patients with MS from a heart attack and stroke. Simultaneously with monitoring the status of patients and their biological age was carried out to estimate the level of chromosomal aberrations and the level of DNA repair in order to identify one of the possible mechanisms of normalization of biological age in persons with MS when applying the inventive method.
The inventive method is carried out as follows.
To create a negative energy balance in the body, patients are advised individually selected low-calorie diet.
Calorie daily diet individually for each patient with MS is calculated in kcal by the formula:
for women 18-30 years (0,0621 × body mass, kg + 2,0357) × 240,
31 - 60 years (0,0342 × body mass, kg + 3,5377) × 240,
over 60 years of age (0,0377 × body mass, kg + 2,7545) × 240,
for men 18-30 years (0,0630 × body mass, kg + 2,8957) × 240,
31 - 60 years (0,0484 × mA the sa body, kg + 3,6534) × 240,
over 60 years of age (0,0491 × body mass, kg + 2,4587) × 240.
The resulting coefficient remains unchanged with minimal physical activity, multiplied by 1.3 in moderate and 1.5 - high physical activity (usually in patients with MS, the level of physical activity low).
It is recommended that 3 main meals and 2 intermediate. With regular skipping one meal significantly increases the incidence of obesity, also revealed a positive correlation between obesity and skipping Breakfast.
It is recommended that the following distribution of daily calories: Breakfast - 25%, 2nd Breakfast - 10%, lunch - 35%, tea - 10%, dinner - 20%.
To compile menu the patient uses a special table with the instructions of the chemical composition and caloric content of products, given that the main sources of energy: protein (1 g contains 4 kcal), fat (9 kcal), carbohydrates (4 kcal) and alcohol (7 kcal).
The second component of the proposed method is feasible regular physical exercise.
In real life you can implement the three forms of physical activity: 1) morning hygienic gymnastics (charging); 2) health care gymnastics; 3) fractional exercise during the day.
1. Morning hygienic gymnastics (charging).
UTRAN is her gymnastics should do after waking up to Breakfast in a ventilated room. Under the influence of training the body is released from the state of inhibition of the physiological processes taking place during sleep.
The sequence of the exercises involves the alternation of load on different muscle groups (arms, legs, body). Exercises are performed with gradually increasing excursion breathing, stretching, limited use of power conditions. Breathing exercise should be free, rhythmic, without delay, mainly through the nose; the exhalation should be longer than the inhale (breath in 2-3 sec, exhale 3-5).
In good health the load, you can increase the number of repetitions faster, increase range of motion and shorter pauses between exercises.
When fatigue should reduce the load, increase the duration of pauses between exercises, to fill their quiet breathing.
After gymnastics, you must go to the rubbing or take a shower.
Morning exercises should evoke a sense of vitality, increase activity, improve well-being and health.
If you feel unwell, develop shortness of breath or pain in the heart and behind the sternum should be temporarily suspend classes and consult a doctor.
During the day, patients with MS recommended treatment and preventive exercises. Depending on the initial fitness applicatio is but the application in the beginning of lightweight option exercise, and later reinforced option.
2. Complexes of preventive and curative gymnastics for people with MS.
2.1. The lightweight version of preventive and curative gymnastics.
Exercise 1. Standing. A quiet walk with a gradual acceleration and deceleration 1-2 minutes on the exercise of the gradual deepening of the breath.
Exercise 2. Circular motion in the shoulder joints. When breeding - breath, when the note - exhale with a slight tilt of the torso and retraction of the abdominal wall 6-8 times. The fingers touch the shoulders, elbows close to the torso.
Exercise 3. Standing, hands on his belt. Alternately shifting the weight of the body from one foot to the other with a small bending the legs at the knee joint without lifting the feet from the floor. Exercise done 10-16 times with the muscles of the legs.
Exercise 4. Standing, feet apart at shoulder width, hands on his belt. Bending the trunk forward, touch right hand to left toe. 4-6 times alternately. When straightened position - breath, when the tilt - exhale. To look ahead.
Exercise 5. Standing with arms along the body. At the same time to take straight arm and one leg to the side with alternating lead leg. 4-6 times in each direction in turn. A wave of the hands and feet, breathe freely, do not delay.
Exercise 6. Standing, feet apart, hands in front of chest with palms down. With a turn of the body to stretch his hands in the pile by turning the palms up - the breath. To return to the original position with a small tilting forward, exhale. 3-5 times in each direction in turn. Breathing to harmonize with the movement of hands and body. By the end of exhalation to engage the wall of the abdomen.
Exercise 7. Standing, hands free. The walk free - 1 min, then with high flexion of the hips - 10-20 movements with subsequent transition to a peaceful walk 1-2 minutes Breathing rhythmic, medium depth.
Exercise 8. Sitting on the edge of the chair, hands on the waist. The bowing of the back and bending forward of the body. Repeat 6-10 times. Breathing is not to delay.
Exercise 9. Sitting on the edge of the chair, the emphasis tassels on the edge of a chair, and your feet slightly in front of the chair. Squats with a focus hands on the edge of a chair - 4-6 times. When you squat exhale.
Exercise 10. Standing sideways to the back of the chair to hold it with one hand. Swing free straight arm with leg pulling back. To return to the original position. Repeat 4-6 times for each leg and arm. Breathing is not to delay.
Exercise 11. Standing, legs apart widely, hands on waist. Turns the body right and left - 6-10 times alternately. The head does not turn, to look forward.
Exercise 12. Standing, feet together, hands down. Move the hands to the sides, inhale, bend the leg at the knee and pressed his hands to his stomach exhale. 3-5 times each leg alternately. Strive to maintain balance; on the exhale, draw your abdomen
Exercise 13. Standing, feet together, hands down. The side slopes of the case with flexion of the opposite hand ("pump"). Breathing is not to delay, to strive for greater excursion of movement.
Exercise 14. Standing. The walk is quiet with a steady breath average depth of 1-2 minutes
Exercise 15. Lying on the Mat to put under your head with a pillow. Go to a half-sitting position and bent to hug the hips, exhale, return to the starting position and inhale. Breath hold. Strive in a semi-sitting position to maintain balance. Run 4-6 times.
Exercise 16. Lying down, arms at sides, legs apart. A semi-circular motion right straight leg to the left with the rotation of the pelvis - exhale - return to the starting position and inhale. 3-5 times in each direction. Alternately left and right, with the greatest possible excursion movement of the leg, keeping the emphasis brushes the floor.
Exercise 17. Sitting on the floor; a) to stand on all fours; b) to move into position on his knees; hands up - breath, torso, and hands to take back - exhale. When the tilt pull the stomach. Repeat 4-6 times; d) to go into a standing position, and son-in-law in the supine position; (e) self-massage of the abdomen small polukrugom movements clockwise - 1-2 minutes Rest 2 min; W) quiet breathing; C) exercise the protrusion (calm) and retraction (active) abdominal wall. Retraction of the stomach is to be combined with the exhale through the mouth. Repeat 4-6 times.
Exercise 18. Standing with arms bent. Energetic walking on the spot or area of high flexion of the hips and waving of hands - 20-30 steps. Breathing is not to delay.
Exercise 19. Sitting on a chair, legs apart, hands on the waist or rest against the edge of a chair. The circular motion of the pelvis with the retraction of the abdomen when moving the pelvis back. 4-6 times in each direction.
Exercise 20. Standing. A peaceful walk with the breath of the average depth of 2-3 minutes
When the source sufficient trained or arising in the course of the practice of tolerance to physical loads you can use the following set of exercises.
2.2. Enhanced preventive and curative gymnastics.
Exercise 1. Standing. The quiet walk with rubbing the palms of the thorax, abdomen, lower back. Runs 1-2 minutes
Exercise 2. Standing, hands down. Simultaneous bending with tension and free "throwing" hand forward, sideways and upwards of 12-16 times. When you bend exhale, when the alignment of breath.
Exercise 3. Standing, hands on waist, legs shoulder-width apart. Lateral motion of the pelvis to the right and left, back and forth. Is 6-10 times alternately. Breathe freely.
Exercise 4. Standing, feet shoulder-width apart, hands pubescent. Circular motion direct hand in the shoulder joints, 6-10 times with a maximum excursion, breathing, do not delay.
Exercise 5. Standing, hands in front of chest, bent at the elbows. Walking with high flexion of the hips, with a touch of the hand. 6 to 10 times for each leg alternately. Motion of energetic, hip flexion - exhale through the mouth.
Exercise 6. The quiet walk 1-2 minutes
Exercise 7. Sitting on the edge of the chair, hands on the waist. Bending and bowing of the body. The exercise is performed 8-10 times. The energetic movement. When straightening - breath, bending exhale.
Exercise 8. Standing sideways to the chair, to hold back, the other hand on the waist. Alternate sweeps straight leg forward, with the greatest possible tour. Swing legs to be combined with the exhalation. Runs 4-6 times.
Exercise 9. Standing, feet apart, hands at sides. Bending the body forward, touch right brush left toe of the feet, the other hand is allotted up. To return to the original position. Tilt combined with exhalation, by straightening the breath by 4-6 times.
Exercise 10. Walking the quiet breathing of the average depth of 1-2 minutes
Exercise 11. Standing, hands in a fist and lowered: (a) bending of the arms at the elbows with the power and extension of their diverting ago, with possibly large rectification and voltage and delay in the stress state within 2-3 C. Standing, hands on waist; b) proprietary with subsequent lifting in socks and tension legs. The exercise is performed 4-6 times with possibly large straighten and what aprazolam, and delay in the stress state within 2-3 C.
Exercise 12. Sitting on the edge of the chair. In the raised position and bent knee leg to make alternate shaking the hands of the calf muscles and thighs. To reduce muscle tension, 1-2 minutes
Exercise 13. Sitting on the edge of the chair, straight leg divorced. Pinch open and direct the hands and feet. When breeding breath, when the note - exhale. Is 4-8 times.
Exercise 14. Standing. The quiet walk with the transition to the rhythmic quiet running (Jogging) on the spot or moving. For fatigue to go on a peaceful walk with the breath of the average depth of 2-5 minutes
Exercise 15. Lying on the Mat. Simultaneous lifting of the body and legs on the exhale, 4-6 times. Arms and legs should be straight, to keep the balance.
Exercise 16. Lying on the Mat, the emphasis brushes the floor. Cross movement straight leg - scissors, 4-8 times. Breathing is not to delay.
Exercise 17. With a quick straightening of the legs to move into a sitting position, then to lie down. You can help stroke 4-6 times.
Exercise 18. Lying on your back, legs bent. Calm breathing 4-6 times.
Exercise 19. Lying on his back, hands diluted with emphasis brushes the floor. With the rotation of the pelvis to the left, bend the legs, rotate the pelvis with legs crossed to the right and stretch the legs. To repeat such a circular motion feet 3-6 times in each direction paperimedioista hand off the floor. Breathing is not to delay.
Exercise 20. Lying on your back, arms and legs separated. Alternate turns the body right and left laying one hand to the other. Feet are not slipping. When divorced hands - breath, turning exhale. Performed 3-6 times.
Exercise 21. Lying on your back, arms along the body: a) turn on your left side; b) to turn on the right side; C) switch to lying on his stomach; to kneel; d) to move in a standing position; (e) walking on the spot with high flexion of the hips and the movement of the hand - 20-30 steps; g) to sit on the Mat; C) lie on your back, relaxing breath. The whole complex repeat 3-4 times.
Exercise 22. Standing. Walking the quiet breathing of the average depth of 1-2 minutes
Exercise 23. Standing, feet apart on the width of the foot, hands on waist. Circular motion of the pelvis, right and left 6-8 times. Breathing is not to delay.
Exercise 24. Standing with legs wide apart, hands in front of chest. Alternately bending the legs with the transfer to it of gravity of the body with stretching hand to toe of the bent leg. 4-6 times in each direction. The other leg is straight. When straightening - breath, when the tilt - exhale.
Exercise 25. Standing, feet apart at shoulder width, hands on waist. Circular head movements by 4-10 times in each direction.
Exercise 26. Standing, feet apart at shoulder width, hands on waist. Bend trunk to the right, with Giva left hand up breath. Return to starting position - exhale. 4-6 times alternately.
Exercise 27. The same hands to the shoulders. Circular motion of the brushes with the maximum excursion in the shoulder joints, when breeding - breath during the mixing and retraction of the abdominal wall exhale. Breathing deep.
Exercise 28. Standing. A peaceful walk and breath the average depth. The reduction of the total load, 1-2 minutes
3. Fractional exercise during the day.
The patient with MS is sick with a number of related disorders, primarily the cardiovascular system. This circumstance limits the simultaneous application of a high physical load during morning exercises, as well as in the main lesson, curative gymnastics. This is why increasing the physical activity of individuals with MS should be implemented by the distribution of physical activity in small doses throughout the day. This method of increasing physical activity in patients with MS should be considered as a method of fractional loads.
Depending on the real conditions of life of patients with MS, patients with various degree of abdominal obesity, you can recommend various exemplary embodiments of the fractional loads to increase physical (motor) activity during the day.
Exercise 1. Sitting - dilute elbows to the side of breath, extend hands forward voltage exhale, 4-6 times.
Exercise 2. Sit - to stand with the deflection of the housing and land - 8-10 times.
Exercise 3. Sitting mimicking the movements of a boxer with possibly a large turn of the body is 10-12 times.
Exercise 1. Standing - walking with high flexion in the hip - 16-30 times.
Exercise 2. Standing - circular head movements to the right and left - 3-8 times in each direction.
Exercise 3. Standing legs apart widely imitated the movements of Costa - 10-12 times.
Exercise 4. Sitting - transition into the standing - 10-12 times.
Exercise 1. The quiet walk - 1 min
Exercise 2. Walking fast - 1 minute
Exercise 3. Walking with high flexion of the hips and alternate wave of the hands 20-30 times.
Exercise 4. Walking the quiet breathing of 1-2 min; quiet running on the spot or move around the room - 2-5 minutes
Walk the pedestrian varying length.
Physical work in different ways and with different duration depending on the conditions (cleaning, home services, cleaning, gardening work, and others).
In addition to custom-made non-medical treatment for patients with MS on the background of stable anti-hypertensive therapy should be prescribed Metformin at a dose of 500 mg 2 times a day after meals. The drug is capable of fra is fucking insulin resistance, that combined with a low-calorie diet and a complex of physical activity normalizes blood lipid profile, lipid peroxidation and platelet hemostasis, reducing the pathologically increased biological age of the patients. In addition, in the medical complex includes Essentiale Forte 1 caps. 3 times, tocopherol acetate 0.1 g 1 caps. 2 times a day and as the sorbent is activated carbon at night on 1 tab. 10 kg weight in 3 hours after meals and other drugs. This complex is essential to reducing the overall pathological family history and pathologically increased biological age in patients with MS with normalization of DNA repair and breakage of chromosomes.
The level of chromosome breakage is defined as follows (Modern methods of chromosome analysis in clinical and cytogenetic studies / Casaitalia, Npoles, Dveselitem, Abbazia: Russian honey. Acad. poledit. image. - M.: Medicine, 1994. - 68 C.).
Cultivation of blood and preparation of preparations of metaphase chromosomes carried out according to the standard technique (guidelines / QI-HC. - M.: Medicine, 1989. - 113 S.; Bochkov N.P. Culture of lymphocytes as a test object for the study of genetic effects in persons exposed to mutagens / Neprochnov, Ussurka, Npoles // Dokl. THE USSR ACADEMY OF SCIENCES. - 1974. - 218 so. No. 2. - S-465). The blood is taken from the C cubital vein (10 ml) and fill it with sterile penicillin vials, which was a solution of heparin (0.1 ml per 1 ml of blood). Heparin is used in breeding pedestrianonly water 1:20. Blood cultured in sterile penicillinase bottles with a nutrient medium at 37°C for 72 hours. Nutrient medium consisting of medium 199 (6,0 ml) and bovine serum (1.5 ml). As a stimulant of proliferative activity using phytohemagglutinin. 2 hours before fixation in lymphocyte culture was added colchicine at a final concentration of 0.5 μg/ml
Fixation is performed on 72 hours of cultivation. After centrifugation and aspiration of the supernatant liquid cells incubated with pre-warmed to 37°With a 0.75 M solution of potassium chloride for 20 minutes at 37°C. After hypotonically spend centrifugation and the selection of the supernatant liquid. Cells fixed in the release of Carnoy (methanol + acetic acid) in the ratio 3:1 for three or more hours. During this time, make three changes of fixative. After complete bleaching of the cell suspension, its dig on fat-free slides, moistened with distilled cold water. Preparations are dried in air and encrypt.
The planting, cultivation of blood lymphocytes and cooking preparations carried out strictly standard in all cases. After cooking preparations remove the more at room temperature for routine staining to study the level of chromosomal aberrations - 2-3 days.
For research on mutagenesis, chromosome preparations stained with dye Romanovsky-Gimza water in the ratio 1:50, without pre-treatment. Time coloring in our studies, 10 minutes
Finished products studied under the microscope, with one person in our study was observed 200 metaphase plate. The norm of the estimated indicators of the level of chromosomal aberrations following : the number of cells with chromosomal aberrations of 1.05 to 1.12 per 100 cells, the number of chromosomal aberrations 1,08-1,12 100 cells, the total number of chromosome fragments 1,36 is 1.48 per 100 cells, the number of exchanges 0,09-0,14 100 cells, the number of single fragments of 0.48-0,54 100 cells, the number of paired fragments of 0.40 to 0.44 per 100 cells, the number of chromosomal exchanges of 0.05-0.08 per 100 cells, the number chromatide exchanges 0.04 to 0.07 per 100 cells.
DNA repair was assessed in lung I.N., Stukalov SV Genotoxic effects on humans in conditions of modern production. In the book: The diversity of modern human genetics. M. 2000. - pagination 126-153).
In linfatico blood was estimated source and indutsirovannymi reparative DNA synthesis (PC). To determine the baseline level PC to 1 ml of whole blood was added 0.6 ml of culture medium, 0.2 ml of 50 mm solution of hydroxyurea and 0.2 ml of N3-thymidine (100 ICC is/ml). When determining the induced level PC, in addition to these substances were added to the solution tiofosfamida to a final concentration of 1.5 µg/ml Cells were incubated at 37°C for 3 hours, after which the tube was added a cold culture medium and placed in the refrigerator. To determine the level of inclusion H3-thymidine, the cells were put on glass filters, which are washed twice with Hanks solution, then a 5% solution of trichloroacetic acid, and dried with absolute alcohol. The filters were placed in vials with scintillation fluid in toluene basis and then in the counter for counting the number of pulses in 1 minute. The degree of induction of PC judged against original PC to PC induced (index reparations). Norm 0,92-0,96.
The biological age of patients is determined by calculating the ratio of the number of electronegative cells to the total number of scraping buccal epithelium when conducting microelectrophoresis (Vgushakov, Allabatou, Twillweave. The method of determining the biological age of a person. Description of the invention to author's certificate SU 1169614). The obtained results are evaluated according to the reference curve (see drawing). The essence of this process explain the examples.
Example 1. Patient K., 63 years old, with body weight 95,0 kg, BMI of 33.3 kg/m2suffering from the metabolic syndr the IOM for 30 years taken 10 ml of blood with the release of lymphocytes and the subsequent estimation of DNA repair (index reparations to 0.80) and the level of breakage of chromosomes (number of cells with chromosomal aberrations 2,52 100 cells, the number of chromosomal aberrations 2,62 per 100 cells, the number of fragments of 1.93 per 100 cells, the number of exchanges of 0.36 per 100 cells, the number of single fragments of 0.95 per 100 cells, the number of paired fragments 0,98 100 cells, the number of chromosomal exchanges 0.19 per 100 cells, the number chromatide exchanges 0.19 per 100 cells). After a night of rest, the patient was taken the sample of buccal cells. A sample of cells, which have previously added to 0.5 μl of water was placed in a cell of microelectrophoresis. Included a constant voltage source, providing on the electrode chamber current of 0.1 ml, and within 5 min were observed under the microscope, by change of polarity, with the offset of cell nuclei in the electric field in five fields of view. The number of electronegative (EA) engines amounted to 20 per 100 cells (20%). Passport age of surveyed persons 63 years. This indicates that the result should be compared with the right branch of the reference curve to determine the biological age. Putting a value on the curve EA and projecting on the abscissa, we find the age corresponding to the age of 82. This indicates that this person older than his passport age of 19 years. The patient was assigned individually selected low-calorie diet (1519,2 kcal), daily physical activity, Metformin 500 mg 2 times the ducks, Essentiale Forte 1 caps. 3 times, tocopherol acetate 0.1 g 1 caps. 2 times a day, and activated carbon, 9.5 tablets at night. The patient was examined after 4 and 8 weeks after starting treatment. Simultaneously with the inspection was carried out the determination of the biological age.
To 8 week of treatment, the patient had decreased body weight (79,0 kg) and body mass index was 27.7 kg/m2normal DNA repair (index reparations 0,93) and the level of breakage of chromosomes (number of cells with chromosomal aberrations at 1.08 on 100 cells, the number of chromosomal aberrations 1,11 100 cells, the number of fragments to 1.38 per 100 cells, the number of exchanges 0,11 100 cells, the number of single fragments 0,52 100 cells, the number of paired fragments 0,42 100 cells, the number of chromosomal exchanges 0.07 per 100 cells, the number chromatide exchanges 0,04 100 cells), which contributed to the normalization of the electronegativity of the nuclei of cells (35%), testified under biological and chronological age in this sick as a result of treatment. The patient is asked to comply with these recommendations in future.
Example 2. Patient K., 46 years old with body mass 85,0 kg, body mass index and 32.3 kg/m2suffering from metabolic syndrome for 8 years under examination revealed the weakening of DNA repair (index repair of 0.82) and level lacost the chromosome (the number of cells with chromosomal aberrations 2,40 per 100 cells, the number of chromosomal aberrations to 2.57 per 100 cells, the number of fragments of 1.92 per 100 cells, the number of exchanges of 0.39 per 100 cells, the number of single fragments of 0.95 per 100 cells, the number of paired fragments of 0.97 per 100 cells, the number of chromosomal exchanges 0.19 per 100 cells, the number chromatide exchanges 0,20 per 100 cells). Found that the rate electronegative nuclei, defined as in example 1, amounted to 43% of the biological age of 65 years. The patient was assigned individually selected low-calorie diet (1543,2 kcal), daily physical activity, Metformin 500 mg 2 times a day, Essentiale Forte 1 caps. 3 times a day, tocopherol acetate 0.1 g 1 caps. 2 times a day, and activated carbon 8,5 tab. on the night. The patient was examined after 4 and 8 weeks after starting treatment. Simultaneously with the inspection was carried out the determination of the biological age.
To 8 week of treatment, the patient had normal body weight (74,2 kg) and body mass index (28,3 kg/m2), was achieved normalization of DNA repair (index reparations 0,96) the normal level of breakage of chromosomes (number of cells with chromosomal aberrations 1.07 per 100 cells, the number of chromosomal aberrations 1,09 per 100 cells, the number of fragments of 1.39 per 100 cells, the number of exchanges 0,09 100 cells, the number of single fragments of 0.50 per 100 cells, the number of pairs is of Rahmanov 0,41 100 cells, the number of chromosomal exchanges 0.06 per 100 cells, the number chromatide exchanges of 0.05 to 100 cells), which provided for a reduction in biological age level calendar. The electronegativity of nuclei was 58%, which indicated that the biological age of the patient was equal to 46 years.
The patient is recommended to observe her prescriptions in later.
The use of the proposed method of correction of biological age in gerontology, cardiology and endocrinology will help to avoid many complications in patients with metabolic syndrome, to reduce their number of cases of temporary disability, to reduce the duration of hospitalization, reduce morbidity and mortality.
The way to reduce pathologically increased biological age in patients with metabolic syndrome through the use of individualized low-calorie diet, a rational dosed static and dynamic physical loads, the introduction of Metformin 500 mg 2 times a day after meals, Essentiale Forte 1 capsule 3 times a day, tocopherol acetate 0.1 g 2 times a day, and activated charcoal 1 tablet per 10 kg of weight overnight.
SUBSTANCE: invention refers to production of genetically unmodified fibroblast cell cultures, multipotent mesenchymal stromal cells, marrow mononuclear leukocytes, and to production of combined transplant with matrix-carrier. Invention represents combined transplant for correction of connective tissue defects, characterised by that it contains cell cultures of marrow mononuclear leukocytes or multipatent mesenchymal stromal cells or human fibroblasts and matrix-carrier, produced by mixing of specified components, thus substratum is laminated with cell suspension of allogenic or autogenic cell culture at rate 5-10 million cells per 1 cm3, substrata with attached cells are wash out in Hanks' medium.
EFFECT: invention provides evident stimulating action on connective tissue growth within engraftment area.
6 cl, 1 ex
SUBSTANCE: troxevazin (Troxerutin) is offered as agent for sports efficiency improvement. Some sportsmen showed maximal oxygen consumption increased after troxevazin application. Invention is distinguished that troxevazin has various action for sports efficiency, and it depends on individual physiological behaviour of sportsman.
EFFECT: improved productivity of cardio-vascular system and increased level of muscular aerobic productivity.
1 tbl, 4 ex
SUBSTANCE: invention refers to intensive care of neurosurgery patients and can be used for treatment of diencephalic-catabolic syndrome (DCS) for patients suffering from central nervous system (CNS) affection. Continuous intravenous infusion of Clonidine in dose 0.05-0.3 mkg/kg/h is performed till vegetative stability is achieved that is body temperature not higher 38°C, heart rate not more than 100 beats per minute, arterial pressure at "working" level or not higher than 20% of these factors.
EFFECT: method improves efficiency of treatment and reduces number of complications for patients suffering from central nervous system affection.
SUBSTANCE: antibiotic in single average-therapeutic dose is dissolved in 0.9% NaCl solution NaCl and 5% KCl solution, thus 100 ml of 0.9% NaCl corresponds to 2-5 ml of 5% KCl. Blood is sampled with syringe and settled directly in syringe. Settled plasma is squeezed out with piston. Produced antibiotic solution is taken to syringe with cellular mass. Produced suspension is mixed and incubated in thermostat at temperature above 36.6°C. Blood volume is restored in syringe to initial value with sterile 0.9% NaCl solution.
EFFECT: method increases antibiotic concentration in blood corpuscle.
SUBSTANCE: invention refers to medical products and concerns application of 3-hydroxi-7-hydroxysteroid for protection from ischemic affection of peripheral organs and for treatment of spinal cord affection caused by spinal cord damage, where steroid is compound of formula and its pharmaceutically accepted salts and esters. Method of mammal protection from ischemia-induced tissue affection in peripheral organs or from damage-induced spinal cord affection by introduction of effective amount of 3-hydroxy-7-hydroxysteroid or its pharmaceutically accepted esters is also disclosed.
EFFECT: given invention extends range of means for protection from ischemic tissue affection in peripheral organs where each of R1, R2, Ra, Rb, A and n has values specified in description.
17 cl, 6 dwg, 14 tbl, 23 ex
FIELD: medicine; surgery.
SUBSTANCE: sclerotherapy of innocent tissue mass is carried out by percutaneous introductions of 10% calcium chloride spirit solution in amount sufficient to achieve medical effect.
EFFECT: method provides full destruction of mass cells within preparation introduction zone and excludes necessity of repeated manipulations in previously sclerosed sites.
2 ex, 8 dwg
FIELD: medicine; pharmacology.
SUBSTANCE: invention refers to medicinal agents containing combined inhibitor of dipeptidylpeptidase IV (DPPIV) and biguanide agent. Offered application of pharmaceutical agent implies production of preventive and therapeutic agent and method of intensified effects of active circulating GLP-1 and/or active circulating GLP-2. Besides, invention concerns method of prevention or treatment of disease connected with active circulating GLP-1 and/or active circulating GLP-2, specifically diabetes, obesity, hyperlipidemia, gastrointestinal diseases. Combined inhibitor DPPIV of formula (I) and biguanide agent, specifically phenformine, methformin or buformine stimulates action of active circulating glucagon-like peptide-1 (GLP-1) and/or active circulating glucagon-like peptide -2 (GLP-2) and, therefore inhibits destruction of GLP-1 and GLP-2, with their levels raised up with biguanide agent.
EFFECT: agent has improved efficiency.
17 cl, 12 tbl, 425 ex
FIELD: chemistry; pharmaceutical compositions.
SUBSTANCE: imidazole derivatives have general formula (I) where each of R1 and R2 independently represents hydrogen, (C1-C6)alkyl or (C3-C8)cycloalkyl; n=0.1 or 2; each of R3, R4, R5 and R6 independently represents hydrogen or (C1-C6)alkyl, halogen, cyano, (C1-C6)alkoxy, trifluoromethyl, (C1-C6)alkylthio, (C1-C6)alkylsulphonyl or (C1-C6)alkoxycarbonyl; R3 and R6 together with their carrier phenyl ring can also form N-methylbenzotriazole and to its acidic-additive salts, solvates and stereoisomer forms. Also, the given invention relates to pharmaceutical compositions, inhibitory aromatiser and application of derived imidazole of formula (I) for producing of medication.
EFFECT: new compounds, with useful biological properties.
23 cl, 2 tbl, 33 ex
SUBSTANCE: invention pertains to new 2,7-substituted indoles of formula: its pharmaceutical salts, where n represents 0, 1 or 2; p represents 1 or 2; R1 represents phenyl, optionally substituted with one or two substitutes, chosen from a group, including halogen, C1-C12alkyl, halogenC1-C12alkyl; R2 represents a mono-valent saturated residue, consisting of one ring, containing six ring atoms, one or two of which are nitrogen atoms, and the others are carbon atoms, optionally substituted with one or two C1-C12alkyls; R3 represents H, C1-C12alkyl. The compounds have antagonist action to the "5-ГТ6" receptor.
EFFECT: possibility of using these compounds in pharmaceutical compositions in an effective quantity.
8 cl, 2 tbl, 8 ex
SUBSTANCE: substance of invention includes application of ligand associated with carrier, where ligand is protein FasL, or Fas leukocyte receptor-directed antibody, to reduce leukocyte activity for extracorporal application. Besides, this invention provides method of leukocyte activity reduction my means of specified module.
EFFECT: damaging action of associated activated leukocytes is inhibited by inactivation module within minutes.
9 cl, 1 ex, 1 tbl, 1 dwg
SUBSTANCE: carbonic mesoporous hemosorbent in the form of smoothed granules of the pseudo-spherical form is proposed. They consist of nano-dispersed of a carbon-carbon material in the form of a three-dimensional matrix, formed curved by layers of carbon of thickness 30-400 Å with radius of curvature 100-1600 Å, interplanar spacing of the layers of carbon is 3.48-3.60 Å which contains not less than 99.5% of carbon, and has density by helium of 1.80-2.08 g/cm3, iodine number 175-245 mg/g, on a specific area on BET 300-400 m2/g, the strength of granules during abrasion is not more than 0.30%/min, total volume of pores not less than 0.4 cm3/g, average diameter mesopore 4.0-20.0 nanometers, it is characterised by the fact that hemosorbent contains granules with the size of 0.3-1.0 mm, and the quantity of granules in the size of 0.3-0.5 mm makes 11-45%, and the content of ashes is no more than 0.08%. Hemosorbent possesses high chemical cleanliness, high mechanical durability of granules, smoothed surface of granules and absence of a dust that gives to it increased blood-compatibility and inertness in relation to the regular elements of blood and provides good dynamics of the sorption cleaning, and prevailing presence mesopore and transitional pores provide a high degree of cleaning blood from low and medium molecular toxins.
EFFECT: hemosorbent has high resistance to sterilisation; is nontoxic and is apyrogenous
3 ex, 1 tbl, 2 dwg
SUBSTANCE: method involves aqueous mineral solution inhalations. The solution is prepared by drawing shungit on mineral water. Inhalation is carried out with solution temperature of 20-24°C within 10 min every day during 7-14 days.
EFFECT: enhanced effectiveness of treatment; reduced treatment cost.
SUBSTANCE: claimed adsorbent contains spherical activated carbon having size diameter of 0.01-1 mm, specific surface determined in accordance to Langmuir equation of 1000 m2/g or more, ratio of diffraction intensities R of 1.4 or more (R is determined as R = (I15-I35)/(I24-I35) (1), wherein I15 is diffraction intensity at diffraction angle (2θ) of X-ray diffraction is 15°C; I35 is diffraction intensity at diffraction angle (2θ) of X-ray diffraction is 35°C; I24 is diffraction intensity at diffraction angle (2θ) of X-ray diffraction is 24°C), and pore volume having diameter of 7.5-15000 nm is less than 0.25 mg/ml. In another embodiment claimed adsorbent contains abovementioned spherical activated carbon with modified surface. Also disclosed are pharmaceutical composition and method for prevention or treatment of kidney or liver diseases containing said adsorbent.
EFFECT: new adsorbent for peroral administration.
20 cl, 5 ex, 2 tbl, 12 dwg
FIELD: pharmaceutical industry.
SUBSTANCE: claimed adsorbent contains spherical active carbon, obtained from thermosetting resin as carbon source, having particle size of 0.001-1 mm, specific surface determined by Langmuir adsorption equation of 1000 m2/g or more and pore volume of 7.5-15000 nm in diameter less than 0.25 ml/g. Also disclosed is adsorbent being similar to abovementioned one, wherein total content of acidic groups is 0.40-1.00 meq/g; total content of basic groups is 0.4-1.1 meq/g. Pharmaceutical compositions contain said adsorbents and pharmaceutically acceptable carriers and recipients. Agents of present invention are useful in treatment of kidney or liver diseases or disorders associated with uremic substance by administration of said adsorbents.
EFFECT: products of increased selectivity.
21 cl, 5 ex, 2 tbl, 11 dwg
FIELD: biology and medicine, in particular therapy of viral and tumor diseases.
SUBSTANCE: claimed method includes exposing of viruses or cells with siglet oxygen generating from molecular oxygen by using of carbon photosensitizing agent contacting with molecular oxygen and said viruses or cells and irradiating with optical radiation. As photosensitizing agent slurry of polyhedral layered carbon nanostructures having interlaminar distances of 0.34-0.36 nm are used.
EFFECT: nanostructures with high heat and radiation resistance, precipitation resistance and relatively low cost.
10 cl, 3 ex
FIELD: medicine and medical chemistry, in particular treatment of exo- and endogenous intoxication.
SUBSTANCE: hemosorbent is treated with 0.005 N potassium permanganate solution; wherein sulfuric acid is used to produce acidic medium. Before heat treatment liquid phase is removed, heat treatment is carried out for 10 min, then hemosorbent is cooled, washed with distilled water and dried in air.
EFFECT: detoxication process of increased effectiveness; treatment with reduced time and cost.
3 ex, 4 tbl, 1 dwg
FIELD: medicine, endocrinology, surgery.
SUBSTANCE: it is necessary to perform intra-pelvic novocain-antibacterial blockade by a certain technique at keeping a catheter in pelvic fiber for further prolonged novocain-antibacterial blockade, then it is necessary to fulfill necrectomy and lance purulent congestions followed by treating with VIER in flow-aerosol mode and application of a sorbent OUT-M till the transition of early wound process into the stage of proliferation; in case of dressing procedure one should apply prolonged intra-pelvic novocain-antibacterial blockade; after transition of early wound process into the stage of proliferation one should treat the wound with VIER in aerosol mode till application of the secondary sutures. The present innovation enables to remove degenerated substances from wound surface very quickly, improve microhemodynamics and lymphodynamics that, in its turn, favors rapid transition of wound process into the stage of proliferation and, thus, the quickest wound healing.
EFFECT: higher efficiency of therapy.
2 cl, 1 ex
FIELD: medicine, pharmacy.
SUBSTANCE: invention relates to preparing medicinal and biologically active substances that can be used for carrying out the supporting therapy. Agent for supporting therapy comprises ultradispersed diamonds or nanodiamonds in the concentration 10-9-10-10 g/kg of the preparation providing the enhancement of activity of preparation of the natural origin and synthesized artificially and used in treatment and prophylaxis of diseases. Invention provides enhancing effect of medicinal agents.
EFFECT: enhanced and valuable medicinal properties of agent.
FIELD: medicine, pharmacy.
SUBSTANCE: invention relates to a pharmaceutical composition used for stabilization of homeostasis and arresting pathological processes in the body. Invention proposes a pharmaceutical composition as powder with particles size from 250 to 400 mcm comprising the following components by the first variant, wt.-%: carbon, 10.01-53.02; oxygen, 30.10-53.10; potassium, 0.26-1.99, and calcium, 0.20-31.37, and comprising the following components by the second variant, wt.-%: calcium, 0.35-31.20; carbon, 10.99-50.21; oxygen, 34.55-51.03; sulfur, 0.73-14.81, and phosphorus, 0.08-3.30. Invention provides compensation of trace elements unbalance that causes and accompanies many diseases, possibility for stabilization of trace element homeostasis and arresting pathological processes of different etiology.
EFFECT: improved and valuable medicinal properties of composition.
12 cl, 13 ex
SUBSTANCE: treatment includes individual hypocalorific diet, sensibly graduated static and dynamic physical activities. Also 500 mg of metformin 2 times per day, 80 mg of valsartan once per day, and 25 mg of hydrochlorthiazide in the mornings are prescribed. The treatment course takes 6 months.
EFFECT: possibility to avoid complications in hemostatic system.