Filtering and sorbing self-decontaminating material for personal protective equipment from impact of organic phosphorus compounds

FIELD: technological processes.

SUBSTANCE: material has an upper layer designed to prevent access of liquid toxic agent and provided with polyurethane or fluoroolefin component, middle sorbing and self-decontaminating layer that includes ferment with organophosphatehydrolase properties and cross-linked acrylate and lower layer made of woven or nonwoven cellulose containing material designed for contact with skin; where all layers are glued at the following elements ratio (mass percent): membrane and woven component - 10.3-21.4; cross-linked acrylate - 12.7-21.4; polypeptide with organophosphatehydrolase activity - 0.00125-0.4 (by protein); buffer - 7.1-44.8; nonwoven/woven cellulose containing component - 7.4-21.4; antiinfection agent - 0-1.2; adhesive - up to 100 (of total weight of material).

EFFECT: material ensures nonoccurence of vapors of highly toxic organic phosphorus compounds behind the layer of protective material and can be stored for a long period without alteration of maintenance of protective properties.

7 ex, 2 dwg

 

The invention relates to a technology for samoreguliruyuschaya filtering and sorbing material, in particular to a method for filtering and sorbing materials that can be used in the manufacture of high-performance personal protective equipment, including a large number of people in emergency situations (disasters and terrorist acts, from toxic substances highly toxic and toxic substances, in particular organophosphorus compounds, namely from exposure to organophosphorus combat toxic chemical (sarin, soman, V-x) and pesticides.

Today, widely known for a variety of filtering and sorbing materials chemical protective actions protective action of the vast majority of which are based on the use of activated carbon or activated carbon fibers as filtering and sorbing component [RF Patent №2107520 (1998), AV 17/00; RF Patent №2110627 (1998), D03D 1/00, 15/00, A62D 5/00; RF Patent №2281798 (2006), AV 23/02, B01D 39/04; Patent No. 2281800 (2006), A62D 5/00].

Thus, the known filtering and sorbing material, in which the filter element is used layers of woven or non-woven activated carbon fibers. Between the layers are divided particles of thermoplastic material (polyvinylchlorid is a or polyethylene) and active carbon. Further layers are connected to each other when heated.

The filter element can additionally have a layer of glass fiber as a reinforcing - mesh canvas cotton [the United Kingdom Patent No. 1476761, B01D 46/00, 46/10, 46/52].

The main disadvantages of this material are:

the use of water-insoluble polymer compounds for fusing to the paper sorbent (activated carbon), which leads to the loss of sorption capacity;

the need to introduce multiple layers of carbon fibers and a reinforcing layer to increase the mechanical strength of the material as a whole.

It should be noted that regardless of the method of producing protective materials based on activated carbon of their total significant disadvantage is the possibility of desorption of toxic substances, as well as the limitations of the protective properties of the sorption capacity of activated carbon (carbon fibers) and, as a consequence, the limited duration of the period of time during which guaranteed the absence of a toxic chemical vapors over the layer of protective material. This period of time, generally not to exceed 24 hours

In this regard, the most effective for use in personal protection are the materials that provide not only effective sorption and retention of toxic substances, but also to carry out what their their decomposition (degassing).

So, there are various filtering and sorbing protective materials, the mechanism of the protective action of which is based on the use of sorbents containing different substances that catalyze the decomposition of adsorbed toxic substances to less toxic products.

Known protective materials, which as sorbents and simultaneously catalysts for the decomposition of toxic substances are introduced chemically activated oxides of aluminum, zinc, magnesium, titanium, cerium, silver (up to 65% by weight of the sorbent) with the addition of monoperoxyphthalate magnesium (up to 35% by weight of the sorbent) [U.S. Patent No. 5689038 (1997), A62D 3/00; U.S. Patent 6403653, B1 (2002), AK 31/02; 31/08; 47/00; 7/42] or complex salts of the same metals (polyoxometalate) [U.S. Patent 6410603, B1 (2002), AC 31/02; 31/08; 47/00; 7/42].

Such protective materials provide when applied to the surface of the substance VI-x or soman at a concentration of 10 g/m2their neutralization by the primary decomposition products by 59% and 98%, respectively, for 24 hours

However, the use of such chemical catalysts in the composition of the protective materials leads to enormous cost of the materials themselves, as for efficient hydrolysis requires a high concentration of catalysts and a high degree of grinding (grain size up to 250 microns). Furthermore, these catalysts are capable not specific utilizirovat only primary degradation of toxic chemicals, as you know, the danger to humans are not only toxic chemicals, and primary products of their hydrolysis.

The alternative to chemical catalysts introduced into the sorbent composition of protective materials, are enzymes that is peculiar to catalyze the hydrolysis of toxic substances, it is known that the speed of decomposition of the original substances under the action of enzymes exceed the speed of reactions catalyzed chemical reagents [Efremenko E.N., Varfolomeev S.D. Enzymes degradation of organophosphorus neurotoxins. // Success Biol. chemistry. T, 2004, s-340]. This allows you to provide the same degree of decomposition of toxic substances during the same period of time, as in the case of chemical catalysts, but at the same time significantly reduce the concentration of enzymes introduced into the composition of sorbents used as components of filtering and sorbing semidegenerate materials.

The most expedient is the use of enzymes in immobilized form, which provides long-term preservation of catalytic activity of enzymes and simplifies the process of their introduction into the structure of the protective materials. It should be noted that the catalytic efficiency of immobilized enzymes included in the protective materials, is determined by the choice of the Fe is ment and the method of immobilization.

Enzyme organophosphorous (ridiuculous, paraoxonase, phosphotriesterase, EC 3.1.8.1) catalyzes the hydrolysis of P-O, P-F, P-S and P-CN bonds in a wide range of substrates, each representing a different derivatives of phosphoric and thiophosphorous acids [Efremenko E.N., Sergeeva, B.C., Organophosphorous - enzyme that catalyzes the degradation of organophosphorus warfare agents and pesticides. // Izvestiya an. Ser. Chem., 2001, s-1749]. The rate of the enzymatic hydrolysis of a number of organophosphorus neurotoxins by 1-2 orders of magnitude higher than chemical. In this regard, the use of this enzyme in the composition of filtering and sorbing semidegenerate materials is the most promising.

Known filtering and sorbing imagegapsize material, which is a polyurethane sponge containing covalently immobilized enzyme organophosphorous and particles of activated carbon [U.S. Patent No. 6642037, B2 (2003), C12N 11/04; 11/08; C12S 9/00; 13/00]. The inclusion of activated carbon in the sorbent and the immobilization of the enzyme is carried out directly in the polymerization process and the formation of a polyurethane carrier. To do this, prepare a mixture of two phases (at a weight ratio of 1:1): the aqueous phase, consisting of 1-2% solution of surfactant (Pluronic P-65, P-85 or L-62), prepared on the basis of 50 mm phosphate is the buffer (pH 8.0), and the solution purified enzyme, mixed together in a ratio of 10:1 by volume, and a hydrophobic phase, representing polyurethane prepolymer Hypol TDI 3000 or Hypol Plus FHP-5000 (tolyl-diisocyanate). The resulting two-phase system intensively stirred (2500 rpm), whisking in the foam for 0.5-1 min due to the feed in the reaction mixture stream of carbon dioxide, and leave for sidezym enzymatic formation of polymeric compositions within 10 minutes, the resulting material is washed thoroughly with 50 mm phosphate buffer (pH 8.0), dried (within 6 h) and stored in sealed form at 4°C. the Maximum concentration of the enzyme in the composition of the material is 8 mg/cm2. This material is intended for detoxification of toxic substances such as sarin, soman and VI-x and can be placed between several layers of polyester to be used in the composition of personal protective equipment [K. LeJeune, Dravis B.C., Yang F., Hetro A.D., Doctor, B.P., Russell A.J. Fighting nerve agent chemical weapons with enzyme technology. // Annals. NY Acad. Science, 1998. V.864, p.153-170].

The developers of this material in the text of the patent does not lead characteristics of the material with respect to its ability to retain and degassing any toxic chemicals. An analysis of several articles by the same authors, partially revealing the properties of the material with immobilized organophosphorous [LeJeune CE, Mesiano A.J., Bowr S.B., J.K. Grimsley, Wild, J.R., Russell A.J. Dramatically stabilized phosphotriesterase-polymers for nerve agents degradation. // Biotechnol. Bioeng., 1997. V.54, p.105-114; LeJeune KE, Wild, J.R., A.J. Russel Nerve agents degraded by enzymatic foams. // Nature, 1998. V.392, p.27-28; Havens P.L., H.F. Rase Reusable Immobilized Enzyme/Polyurethane Sponge for Removal and Detoxification of Localized Organophosphate Pesticide Spills. // Ind. Eng. Chem. Res., 1993. V.32 (10), p.2254-2258; LeJeune CE, Russell A.J. Biocatalytic nerve agent detoxification in fire fighting foams. // Biotechnol. Bioeng., 1999. V.62 (6), p.659-665], suggests that this material when applied to the surface of a substance paraoxon (pesticide) at a concentration of 0.9 g/m2provides addressing it to the primary decomposition products 100% for 1 h Because it is known that the catalytic efficiency of the enzyme organophosphorous used in the composition of this material, in relation to substances VI-x 105times less in comparison with paraoxon [Kolakowski J.E., J.J. DeFrank, S.P. Harvey, Szafraniec L.L., Beaudry W.T., K. Lai, J.R. Wild Enzymatic hydrolysis of the chemical warfare agent Vx and its neurotoxic analogues by organophosphorus hydrolase. // Biocatal. Biotrans., 1997. V.15, p.297-312; Efremenko E.N., Sergeeva, B.C., Organophosphorous - enzyme that catalyzes the degradation of organophosphorus warfare agents and pesticides. // Izvestiya an. Ser. Chem., 2001, s-1749], therefore, to neutralize the same amount (0.9 g/m2) applied substances VI-x or sarin on the same material will be required, respectively, in 105and 104hours more, that is about 416 and 417 su is OK, respectively.

The obvious reason for low efficiency of the material in relation to toxic substances is extremely low catalytic activity of the enzyme in the composition of the material, is primarily due to the chosen method of immobilization of the enzyme. In the covalent immobilization of the enzyme in the formation of media is the plural formation of covalent bonds between the carboxyl and amino groups of the protein with prepolymers. In addition, low concentration of the aqueous phase (in this case formed due to the presence of phosphate buffer in the material is not more than 1% wt.) in the composition of the material obtained in the microenvironment of the enzyme leads to the presence of difficult catalysis.

Known filtering and sorbing imagegapsize protective material with:

the top layer is made of polypropylene, polycarbonate or bottled rubber in contact with drops of toxic substances, the insulating inner layers of material against penetration of liquid phase toxic substances and providing uniform supply vapour to the inner layers of the material;

the middle layer is designed for sorption of vapors of toxic substances and their degassing and consisting of rubber or foamed plastic impregnated with particles of activated carbon, the enzyme phosphorylacetate and artiodactyl the th acid [U.S. Patent 4781959 (1988), F41H 3/00];

the bottom layer intended for contact with the skin and represents a cellulose-containing material.

The developers of this material did not provide any factual data that reflects the composition of the material components in the mass ratio, as well as evidence of the effectiveness of declared material and reflecting its characteristics. However, analysis of the composition of this samoreguliruyuschaya protective material leads to the conclusion that he has a number of significant drawbacks and may not have satisfactory characteristics as the composition of the material used enzyme, characterized by extremely low non-specific catalytic activity against toxic substances, comparable to the action of chemical reagents at pH 8.0 [I.B. Wilson, J. Dayan The free energy of hydrolysis of phosphoryl-phosphatasa. // Biochemistry, 1965. V.4 (4), p.645-649]. Indirectly, the inefficiency of enzyme confirmed the introduction of the protective material Artiodactyla acid with strong oxidizing properties against various toxic substances and used as a degasser in practice [Yang Y.C., Baker J.A., Ward J.R. Decontamination of chemical warfare agents. // Chem. Rev., 1992, V.92. p.1729-1743; R. Ramaseshan, S. Sundarrajan, Liu Y., Barhate R.S., Lala N.L., Ramakrishna S. Functionalized polymer nanofibre membranes for protection from chemical warfare stimulants. // Nanotechnoloy, 2006. V.17, p.2947-2953].

In addition, with the introduction of Artiodactyla acid (pH<7) the specified enzyme loses its catalytic activity, and, thus, its introduction in the composition of the protective material becomes completely useless, and degassing the action reduces to the action of the chemical agent (orthobenzoquinone acid).

The presence in the microenvironment of immobilized enzyme rubber or foamed plastic and, therefore, no withholding these materials water in the microenvironment of the enzyme should also adversely affect its catalytic properties.

Disadvantages of using particles of activated carbon as a sorbent are possible desorption of toxic substances, and low sorption capacity of this sorbent material.

This solution, as the closest to the claimed purpose and method of production, namely obtaining protective filtering and sorbing samoreguliruyuschaya material consisting of three main layers (protective filter layer absorbing and simultaneously degassing layer containing an enzyme and a layer intended for contact with the skin), adopted for the prototype.

Object of the present invention to provide an effective filtering and sorbing samoreguliruyuschaya mA is Arial for use in personal protection from exposure to organophosphorus compounds.

The problem is solved in that the filtering and sorbing imagegapsize material for personal protection from exposure to highly toxic and toxic organophosphorus substances contains upper-layer insulating against the penetration of toxic substances in liquid form, the average sorbent and imagegapsize layer contains the enzyme, and the bottom layer, made of woven or non-woven cellulose-containing material is suitable for contact with the skin, and the top layer material contains polyurethane or ferolino membranacei component, as a degassing element contains the polypeptide with properties of organophosphites, and as a component, sorbing and retaining degassing element in the buffer contains cross-linked acrylate with sizing all layers of binder material in the following ratio of components (wt.%): membranacea component of 10.3÷21,4; cross-linked acrylate - 12,7÷21,4; a polypeptide with the activity of organophosphorous - 0,00125÷0,4 (for protein); buffer - 7,1÷44,8; cellulose woven/non-woven component - 7,4÷21,4; antimicrobial substance - 0÷1,2; binder to 100 (by weight of the total material).

The use of polyurethane or ferreirola membranaceous component (polyamidoamine the Naya fabric with paletterollupinfo or polyurethane surface, having oleophobic properties) in the protective material allows protection from the liquid phase of various toxic substances, not passing it to the underlying layers and the skin. This ensures uniform distribution and dosage for supplying the vapor phase of various toxic substances to the absorptive layer (see figure 1).

The claimed technical solution as an absorbing and decontaminating layer is used polymeric sorbent, representing a cross-linked acrylate (copolymer of acrylic acid, partially neutralized with a solution of potassium hydroxide, and acrylamide), not soluble in water and organic solvents, swelling to a gel upon contact with aqueous solutions. When the swelling is able to absorb the aqueous phase, increasing its own weight of 3000 times. Thus, the sorbent has a great absorption capacity and ability to hold large amounts of sorbed substances. In addition, this sorbent is completely non-toxic as oral ingestion, and effects on skin and eyes of humans and animals, but it does not show even a minimal irritant to the skin and eyes.

Physical immobilization (sorption) in the amount of absorbent solution of the polypeptide having the properties of organophosphate the s [RF Patent №2255975 (2005), C12N 1/21, 15/52, 15/70], is absolutely new, previously unknown technical solution for creating a chemical protective materials.

First, the polypeptide is characterized by significantly improved compared to the initial enzyme of organophosphorous catalytic characteristics, can be used for hydrolysis of organophosphorous chemical agents in a wider range of pH and temperature, as well as to conduct more effective and rapid hydrolysis of the same substrates [Vaccinia Y.A., Efremenko E.N., Aliyev BECAUSE, Varfolomeev S.D. (2006). Properties hexacitinellidae organophosphites. // Biochemistry, T (2), s-222; Gudkov D.A., Vaccinia Y.A., Efremenko E.N. (2006). Hydrolysis paraoxon catalyzed by organophosphorous containing polyhistidine sequence at the C-end of the protein molecule. // Vestnik MGU, ser. Chem. T (1), p.15-20]. Besides, the presence polyhistidine sequence in the polypeptide molecule provides quick selection of E. coli cells [Efremenko E., Votchitseva Y., Plieva F., I. Galaev, B. Mattiasson (2006). Purification of His6-organophosphate hydrolase using supermacroporous monolithic polyacrylamide cryogels developed for immobilized metal affinity chromatography // Appl. Microb. Biotech. V.70 (5), p.558-563], and therefore, scaling of process developments for use in obtaining the claimed protective material is easily realizable.

Secondly, f is the physical method of immobilization of the polypeptide in contrast to chemical methods, used in counterparts and prototype ensures maximum preservation of the baseline characteristics of the enzyme. The immobilization process is very simple, takes less than 1 minute and consists of mixing a solution of the enzyme in the buffer with the sorbent, which in turn quickly absorbs the solution. In addition, the sorbent, having great sorption capacity, allows you to enter different amount of enzyme in the sorption layer, on the one hand, and, on the other, the sorbent is guaranteed keeps a buffer solution with a given pH in the microenvironment of the enzyme and creates favorable conditions for efficient enzymatic catalysis.

The composition of the buffer, from which is prepared a solution of the polypeptide, are administered antimicrobial substances [Legin GY, Schechtman NM, V.M. Andreev Preservation of cosmetic products and effective modern preservatives. 1983. Issue 3, p.1-36; Anisimov, Y., Gudz O.V., Capowski NI, yalovenko methods H. the Effect of preservatives on the regeneration process of the skin // I-III international. scientific-practical conference "Biologically active substances: new technologies and products in cosmetics", M, 1998, p.40-41], which prevent during prolonged storage of the claimed material development extraneous microflora in its internal layers containing the introduced moisture. The introduction of the protective material antimic is one of the substances, the use of which is permitted, including in children's cosmetics, creates favorable conditions for long-term storage of the biocatalyst in a sealed form in the absence of damage to the finished product and ready to use, helps prevent any risk of exposure to consumers of skin diseases associated with microbial contamination caused by, for example, cultures of Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris and others, and makes a potentially safe use of protective material to the consumer without any age restrictions.

Sorption layer in the present material provides the sorption of vapors of toxic substances that have passed through the upper layer (see Drawing 1), pellets of a polymeric sorbent and subsequent hydrolysis themselves of toxic substances and products of their hydrolysis under the action of the immobilized polypeptide.

Bottom hygienic layer made of woven or non-woven cellulose-containing material is suitable for contact with the skin and functions as the substrate for the overlying layers, and allows removal of moisture vapour away from the body. The use of woven and nonwoven materials based on cotton (calico, gauze, felt, and others) as a porous material due to the fact that they, firstly, a material based on cotton, in the best of the e from synthetic, have a high sorptive capacity and can easily hold large amounts of hydrophilic liquids. Secondly, these materials are produced in large volumes textile industry, and, consequently, the potential production of protective material on the basis of such materials can be easily scaled using the equipment of textile enterprises.

To give the material strength, as well as to ensure uniform distribution of the sorbent in the inner layer composed of a binder material is used, which apply solutions of polymers (gelatin, starch, polyvinyl alcohol, polyethylene glycol and others).

This combination of all the major components of filtering and sorbing samoreguliruyuschaya material as the claimed technical solution, even to the same when the claimed ratios in the composition of the material that previously was not known and allows to characterize the proposed solution is as new.

The following are specific examples of implementation of the proposed technical solution. The protective properties of the material, in particular the penetration of toxic substances through the samples was determined by approved methods (GOST In 16797-76 1976).

Illustration of the proposed technical solution is held on the toxic substances type VI-x, sarin and soman, oxitest which substantially exceeds the toxicity of organophosphorus pesticides (paraoxon, methylparathion, parathion, karbofos, etc.), belong to the same class of substances, which are known to be easily hydrolyzed peptide with organophosphorous activity [Vaccinia Y.A., Efremenko E.N., Aliyev BECAUSE, Varfolomeev S.D. (2006). Properties hexacitinellidae organophosphites. // Biochemistry. T (2), s-222].

Example 1. Filtering and sorbing imagegapsize material containing a highly purified polypeptide with organophosphorous activity and Katon CG as antimicrobial substances.

In 300 ml of 50 mm phosphate buffer (pH of 7.8)containing 0.1 mg/ml purified polypeptide with organophosphorous activity and 0.05% kathon CG, make 100 g of sorbent. Swollen sorbent distribute membranaceum material on the surface of which is pre-applied binder (1% starch solution), on top of the sorbent impose mercerized cellulose woven material containing a binder on its surface, after which all the layers are pressed.

The obtained filtering and sorbing imagegapsize material has the following composition (wt.%): membranacea component - 15,2; cross-linked acrylate - 20,3; a polypeptide with the activity of organophosphorous - 0,00125 (for protein); buffer - 30,4; woven cellulose component to 10.1; antimicrobial substances the STV - 0,03; binder - 100 (mass of material).

Such filtering and sorbing imagegapsize material when applied to the surface of a substance VI-x at a concentration of 10 g/m2provides neutralizing vapors at 22°100% for 5 h at pH of 7.8, ensuring the absence of vapors of toxic chemical after layer of protective material over at least 96 hours. This material retains its protective properties at 100% after storage at +8°in an airtight container for 6 months.

Example 2. Filtering and sorbing imagegapsize material containing a highly purified polypeptide with organophosphorous activity and propylparaben as antimicrobial substances.

In 400 ml of 50 mm carbonate buffer (pH of 10.5)containing 0.2 mg/ml purified polypeptide with organophosphorous activity and 0.1% propyl paraben, make 100 g of sorbent. Swollen sorbent distribute membranaceum material, the surface of which is coated binder (1% solution of gelatin), on top of the sorbent impose a cellulosic non-woven material containing a binder on its surface, after which all the layers are pressed.

The obtained filtering and sorbing imagegapsize material has the following composition (wt.%): membranacea component - 4,3; cross-linked acrylate - 12,7; a polypeptide with the activity of organophosphorous - 0,0076 (for protein); buffer - 38,2; cellulose nonwoven component - 12,4; antimicrobial substance - 0,09; binder - 100 (mass of material).

Such filtering and sorbing imagegapsize material when applied to the surface of a substance VI-x at a concentration of 10 g/m2provides neutralizing vapors at 22°100% for 3 hours, ensuring the absence of vapors of toxic chemical after layer of protective material over at least 96 hours. This material retains its protective properties at 100% after storage at +4°in a sealed package within 8 months.

Example 3. Filtering and sorbing imagegapsize material containing digidrirovanny highly purified polypeptide with organophosphorous activity.

In 500 ml of 50 mm carbonate buffer (pH 10,0)containing 0.5 mg/ml purified polypeptide with organophosphorous activity, contribute 150 g of sorbent. Swollen sorbent together with the enzyme rehydration, drying it at 25°With up to 10%residual moisture, and distribute membranaceum material, the surface of which is coated binder (3% starch solution), on top of the sorbent impose a cellulosic non-woven material containing a binder on with the Oia surface, then all the layers are pressed.

The obtained filtering and sorbing imagegapsize material has the following composition (wt.%): membranacea component - 21,4; cross-linked acrylate - 21,4; a polypeptide with the activity of organophosphorous - 0,0041 (for protein); buffer - 7,1; cellulose nonwoven component - 21,4; binder - 100 (mass of material).

Such filtering and sorbing imagegapsize material when applied to the surface of a substance soman at a concentration of 10 g/m2provides neutralizing vapors at 37°100% for 4 hours, ensuring the absence of vapors of toxic chemical after layer of protective material over at least 96 hours. This material retains its protective properties at 100% after storage at +4°in a sealed package within 12 months.

Example 4. Filtering and sorbing imagegapsize material containing the crude polypeptide with organophosphorous activity and chloride benzene as antimicrobial substances.

650 ml 100 mm CHES buffer (pH 9,0), containing supernatant with a polypeptide having organophosphorous activity obtained after disintegration of cells with a total protein concentration of 2.5 mg/ml, and 1% chloride benzene contribute to 220 g of sorbent. Swollen sorbent distribute membenarkan the first material, on the surface of which is coated with binder (1% solution of polyvinyl alcohol), on the top of the sorbent impose a cellulosic non-woven material containing a binder on its surface, after which all the layers are pressed.

The obtained filtering and sorbing imagegapsize material has the following composition (wt.%): membranacea component - 13,5; cross-linked acrylate - 15,0; a polypeptide with the activity of organophosphorous - 0,11 (for protein); buffer - 44,8; cellulose nonwoven component - 7,4; antimicrobial substance - 0,45; binder - 100 (mass of material).

Such filtering and sorbing imagegapsize material when applied to the surface of a substance sarin at a concentration of 10 g/m2provides neutralizing vapors at 28°100% for 7 h, guaranteeing the absence of a toxic chemical vapors over the layer of protective material over at least 96 hours. This material retains its protective properties at 100% after storage at +4°in an airtight container for 6 months.

Example 5. Filtering and sorbing imagegapsize material containing highly purified registrationentry polypeptide with organophosphorous activity and methylparaben as antimicrobial substances.

350 ml of 50 mm carbonate-phosphate buffer(pH 8.5), containing 5 mg/ml registrationpage of liofilizirovannogo purified preparation of the polypeptide having organophosphorous activity, and 2% of methylparaben, make 150 g of sorbent. Swollen sorbent distribute membranaceum material, the surface of which is coated binder (2% solution of polyethylene glycol), on top of the sorbent impose woven cellulose material containing a binder on its surface, after which all the layers are pressed.

The obtained filtering and sorbing imagegapsize material has the following composition (wt.%): membranacea component - 15,0; cross-linked acrylate - 13,2; a polypeptide with the activity of organophosphorous - 0,22 (for protein); buffer - 40,0; woven cellulose component is 9.8; antimicrobial substance - 0,9; binder - 100 (mass of material).

Such filtering and sorbing imagegapsize material when applied to the surface of a substance VI-x at a concentration of 10 g/m2provides neutralizing vapors at 22°100% for 3 hours, ensuring the absence of vapors of toxic chemical after layer of protective material over at least 96 hours. This material retains its protective properties at 100% after storage at +10°in an airtight container for 4 months.

Example 6. Filtering-SOR is yuushi imagegapsize material, containing disintegrated cells of bacteria containing the polypeptide having organophosphorous activity, and propylparaben as antimicrobial substances.

In 475 ml of 50 mm carbonate buffer (pH of 10.5)containing 10 mg/ml disintegrated cells of E. coli containing the polypeptide having organophosphorous activity, and 3% of propyl paraben, make 210 g of sorbent. Swollen sorbent distribute membranaceum material with carbonaceous nonwoven base on the surface of which is applied binder (3% starch solution), on top of the sorbent impose woven cellulose material containing a binder on its surface, after which all the layers are pressed.

The obtained filtering and sorbing imagegapsize material has the following composition (wt.%): membranacea component and 10.3; cross-linked acrylate - 14,4; a polypeptide with the activity of organophosphorous of 0.4 (for protein); buffer - 32,6; woven cellulose component - 9,4; antimicrobial substance - 1,2; binder - 100 (mass of material).

Such filtering and sorbing imagegapsize material when applied to the surface of a substance VI-x at a concentration of 10 g/m2provides neutralizing vapors at 45°100% for 3 hours, ensuring the absence of vapor toxic the CSOs chemical after layer of protective material over at least 96 hours. This material retains its protective properties at 100% after storage at +8°in an airtight container for 6 months.

Example 7. Degassing properties of filtering and sorbing material.

A polypeptide with the activity of organophosphites, consisting of filtering and sorbing material having the composition described in Example 2, is capable of hydrolysis of not only P-O, P-S and P-F bonds in molecules of toxic organophosphorus compounds, as well as to catalyze the hydrolysis of P-C bond in the molecules of the primary products of hydrolysis of toxic substances. In particular, methylphosphonous acid resulting from the enzymatic hydrolysis of the substance VI-x, gradually accumulates in the degassing process VI-x in the sorbent in the microenvironment of the enzyme and also enzymatic hydrolyzed with the formation of phosphoric acid (see Drawing 2). Thus, in the present material, namely sorption imageguidance layer is deep destruction of the adsorbed toxic substances under the catalytic influence of the polypeptide with organophosphorous activity.

Thus, the inventive filtering and sorbing imagegapsize material has the following advantages in comparison with analogues and prototype:

1. The inventive material is superior counterparts and p is totip in its ability to degassing adsorbed toxic chemicals in 4-8 times in a wide range of pH and temperature.

2. The amount of the polypeptide with the activity of organophosphates used as a degassing element, which is part of the proposed material can be introduced in various forms, is considerably small compared with the number of metal catalysts and biocatalysts used in the models and the prototype.

3. Due to the action of the polypeptide with the activity of organophosphorous part of the claimed material is deeper destruction of highly toxic organophosphorus compounds than analogues and the prototype, before the formation of secondary decomposition products toxic substances (example 7).

4. The inventive material has significantly improved barrier properties compared with the known analogues based activated carbon, in particular for the period of time during which guaranteed the absence of vapors of highly toxic organophosphorus compounds for the layer of protective material is increased at least 4 times.

5. The introduction of the protective material of antimicrobial substances, the use of which is permitted, including in children's cosmetics, creates favorable conditions for long-term storage of biocatalyst in an airtight (up to 12 months) in the absence of damage to the finished product and ready for use by consumers without the but boot restrictions.

Filtering and sorbing imagegapsize material for personal protection from exposure to organophosphorus highly toxic and toxic substances, with the top layer, insulated from the penetration of toxic substances in liquid form, the average sorbent and imagegapsize the layer containing the enzyme, and the bottom layer, made of woven or non-woven cellulose-containing material intended for contact with the skin, characterized in that the top layer contains polyurethane or ferolino membranacei component, as a degassing element contains the polypeptide with properties of organophosphites, and as a component, sorbing and retaining degassing element in the buffer contains a cross-linked acrylate with sizing all layers of binder material in the following ratio of components (wt.%): membranacea component 10,3-21,4; cross-linked acrylate 12,7-21,4; a polypeptide with the activity of organophosphorous 0,00125 of 0.4 (for protein); buffer 7,1-44,8; cellulose woven/non-woven component of 7.4-21,4; antimicrobial substance 0-1,2; binder to 100 (by weight of the total material).



 

Same patents:

FIELD: medical and biological instrumentation industry; methods of modification of the hydrophobic surfaces.

SUBSTANCE: the invention is pertaining to the method of modification of hydrophobic surfaces and may be used for upgrading the hydrophobic surfaces used in the scanning probe microscopy, in the immune-enzyme analysis, at development of the biochips. The method of modification of hydrophobic surfaces includes deposition on the surface of the modifying monolayer of the complex composition molecules containing the functional group, the section forming the hydrogenous ties and the hydrophobic section. At that as the functional group the molecule contains, at least, one group sampled from the following row:NH2, СООН, СНО,ONH2, SH; as the section forming the hydrogen ties the molecule contains the fragments -NH(CH2)nCO- ,where п=1-5, and as the hydrophobic section the molecule contains the fragment -(СН2)n , where n=5-12, or the similar fragment with one or several insertions of the heteroatoms sampled from the row of O, S, NH. In particular, such molecules may have the following composition[Gly4-NHCH2]2C10H20. The invention allows to ensure the high quality evenness of the modified surfaces, reproducibility of their properties and reduction of the time interval of the modification.

EFFECT: the invention ensures the high quality evenness of the modified surfaces, reproducibility of their properties and reduction of the time interval of the modification.

2 cl, 3 dwg

FIELD: chemical technology.

SUBSTANCE: invention relates to particles of a thickened composition based on metal oxide comprising metal oxide and a binding agent, and to a method for forming particles from the thickened composition based on metal oxide. Metal oxide is chosen from iron and zinc oxides and a binding agent from starch, cellulose, carboxycellulose. The composition can comprise an activating agent additionally. The composition is used for removal of sulfur compound from a fluid medium being at temperature less 200°C mainly. Invention provides preparing stable particles showing high sulfur-capacity.

EFFECT: improved and valuable properties of composition.

29 cl, 8 dwg, 8 ex

FIELD: veterinary science.

SUBSTANCE: absorbing material contains at least one calcium compound mixed with plant fibers, and dry residue of distillation of mother liquor delivered from enterprises for production of aqueous ammonia soda. Also described is method for utilization of aqueous ammonia solution. Said material is used for absorption of liquid from ground. Described in Specification is litter for animals comprising such a material having good absorbing capacity for absorption of animal's liquid excrements.

EFFECT: improved liquid absorbing capacity and mechanical strength, and reduced adherence to ground during utilization of litter made from such absorbing material.

9 cl

FIELD: environment protection from radionuclides.

SUBSTANCE: proposed method for producing chemical sorbent to absorb nuclear fuel fission products (radionuclides of iodine, ruthenium, and their volatiles) includes impregnation of activated carbon in triethylene diamine followed by its drying at temperature of 110-130 °C. For the purpose use is made of activated carbon produced from raw bituminous coal having micropore volume vmp = 0.28-0.33 cm3/g and total pore volume vΣ = 0.85-1.0 cm3/g. Activated carbon is impregnated until triethylene diamine content is reduced to 1-2% of base mass. Then chemical sorbent obtained in the process is dried out and sifted.

EFFECT: enhanced quality of chemical sorbent obtained.

1 cl, 1 tbl, 9 ex

FIELD: chromatographic sorbents.

SUBSTANCE: invention relates to chromatographic sorbents, which can be used for analysis and preparative purification of optically active compounds. A novel sorbent for resolution of isomers of optically active compounds is developed containing, as chiral selector, macrocyclic glycopeptide antibiotic eremomycin, vancomycin, ristomycin A, teicoplanine, or their aglycons. Method of immobilization of macrocyclic glycopeptide antibiotics is also developed, which resides in that silica gel in aqueous buffer solution is first treated with 3-glycidoxypropyl(trialkoxy)silane and then, in alkaline aqueous or water-organic solution, above-indicated macrocyclic glycopeptide antibiotic is grafted to epoxy group-modified silica gel.

EFFECT: increased selectivity in enantiomer resolution and simplified preparation procedure.

9 cl, 12 dwg, 5 tbl, 9 ex

FIELD: oil pollution removal.

SUBSTANCE: biological preparation comprises substance carrier, microorganism growth factor, and biomass of microorganisms serving as oil destructors. Carrier is a composition made from Ca alginate gel, C14-C16-n-alkanes, and microorganism growth factor substance. Composition of biological preparation assists localization of microorganism growth density directly in the carrier near to interface between oil product and medium to be treated. Treatment method includes placing indicated dispersed biopreparation with microorganisms on water medium surface.

EFFECT: enabled use of well-workable granules and increased degree of purification.

5 cl, 2 tbl

The invention relates to the field of ecology, in particular the sorption purification of aqueous solutions

The invention relates to the field of processing radioactive liquid wastes and aqueous media containing petroleum products

FIELD: technological processes.

SUBSTANCE: invention is related to the filtration method of grafted polyol dispersions with use of repeated filter system. Invention describes a method of grafted polyol filtering with use of refillable filter system consisting in a) use of refillable filter system that consists of the first tank and the second tank; b) the first part of the positive-displacement filter material is installed between the first and the second tanks thus developing fluid-tight seal is developed between the first tank and filter material; c) dispersion of grafted polyol is loaded to the first tank; d) dispersion of grafted polyol is let to the second tank through filter material, and e) the first part of positive-displacement filter material is removed from the area between the first and the second tanks and the second part of clean positive-displacement filter material with twisting pores is installed between the first and the second tanks. Received filtered dispersion of grafted polyol has particles of maximum size of 25 microns and is stable for various conditions for minimum period of 9 weeks.

EFFECT: method ensures cost effective and fast filtration of large volumes of grafted polyol dispersion.

17 cl, 1 dwg, 3 ex

FIELD: technological processes.

SUBSTANCE: invention is related to the filtration method of grafted polyol dispersions with use of repeated filter system. Invention describes a method of grafted polyol filtering with use of refillable filter system consisting in a) use of refillable filter system that consists of the first tank and the second tank; b) the first part of the positive-displacement filter material is installed between the first and the second tanks thus developing fluid-tight seal is developed between the first tank and filter material; c) dispersion of grafted polyol is loaded to the first tank; d) dispersion of grafted polyol is let to the second tank through filter material, and e) the first part of positive-displacement filter material is removed from the area between the first and the second tanks and the second part of clean positive-displacement filter material with twisting pores is installed between the first and the second tanks. Received filtered dispersion of grafted polyol has particles of maximum size of 25 microns and is stable for various conditions for minimum period of 9 weeks.

EFFECT: method ensures cost effective and fast filtration of large volumes of grafted polyol dispersion.

17 cl, 1 dwg, 3 ex

FIELD: textiles; paper.

SUBSTANCE: multilayered sorption-filtering non-woven material consists of at least two sorption layers of non-woven material. The material consists of 2-4 sorption layers of non-woven material, consisting of fibres, filled with solid sorption particles, obtained from polymer solutions through aerodynamic shaping. The material also contains a reinforcing layer made from woven or non-woven material, on the outside of one or two sides of the sorption layers of the non-woven material. The layer of the reinforcing layer is ranges from 0.1 to 0.5 of the layer of the sorption layers. The ration of the diameter of the fibre of the reinforcing fabric layer to the diameter of the fibre of the sorption layers is about 6-14, 0.4-2 of the non-woven layer, and the ratio the specific surface of the reinforcing layer to the specific surface of the sorption layers is about 0.01-0.1.

EFFECT: increased physical and mechanical properties and simplification of the manufacturing technology.

2 cl, 2 ex

FIELD: chemistry.

SUBSTANCE: multilayer composition is invented where the layers are placed from top to bottom in the following sequence and at the following content of the components, in volume percent: 15-20 of the polyethylene or styrene granules with density not less than 1 g/cm3; 5-10 of the low-basic macroporous anionite with density 1.04-1.08 g/cm3; 10-15 of the impregnated by fulvic acids, with density 1.04-1.12 g/cm3; 43-56 of the strong-acid cation resin with density 1.2-1.3 g/cm3; 3-5 of the filtrating layer consisting of nepheline, aluminum oxide, bentonite and silicon carbide, with density 2.0-2.1 g/cm3; 1-5 of gravel with density 2.0-2.5 g/cm3; 1-5 of calcite with density 2.0-2.5 g/cm3.

EFFECT: complex water purification is provided.

3 tbl, 1 dwg

FIELD: chemistry.

SUBSTANCE: proposed filter for filtering a water stream consists of filtering material made from a water soluble fibrous material, made from polyvinyl alcohol, and a carrier for the fibrous material, in which the filtering material contains a thread or bundle made from polyvinyl alcohol and the carrier has a core. The thread or bundle is wound around the core. A method of filtering a stream of water using the given filter is also proposed.

EFFECT: invention is efficient for eliminating wastes.

20 cl, 5 dwg

FIELD: engines and pumps.

SUBSTANCE: filter element for filter-separators is intended for clearing liquid and gaseous media of impurities and free moisture in the form of aerosols and may be used in petroleum refining and aircraft industries as well as in other industrial branches. The above filter incorporates a filtration and coagulation layers arranged streamwise the media being filtered and is made from thermoplastic polymer fibres, primarily from polypropylene. Note that coagulating layers are made from fine fibres and separated by a layer of coarse fibres, the density of coagulating layers on the side of the incoming medium to be filtered exceeding that on the outlet side.

EFFECT: simpler and cheaper filter design.

1 ex, 1 tbl, 2 cl

FIELD: chemistry.

SUBSTANCE: material for cartridge filter for water purification is proposed, which comprises the layers of the fibrous polymeric material with different packing compactness of interconnected microfibers in layers, formed at the porous frame and containing the sorbent particles in one of fibrous layers where the first layer along the purified water stream has the compactness of fiber packing to withhold the 10-20 mcm particles; the second layer contains the sorbent particles and supplementary chemical reagents to transfer the ferrous iron in ferric iron, hydrolysis and coagulation of hydrolised forms, the third layer has the compactness of fiber packing to withhold the 5-20 mcm particles; the fourth layer has the compactness of fiber packing to withhold the 1-2 mcm particles; and the fifth layer comprises the fibrous non woven bottom layer upon the porous frame. The method of preparation of the filter containing the invented sorption-filtering material is proposed.

EFFECT: invention provides high degree of water purification of iron and heavy metals.

6 cl, 1 dwg, 2 tbl, 5 ex

FIELD: physics.

SUBSTANCE: process for highly remanent fibrous composite production includes extrusion forming and subsequent pneumatic extrusion processing of granulated polyethylene filled with powdered strontium ferrite with mass concentration up to 30 %; before extrusion forming the filling material is during 10 minutes mixed with dioctylphtalate (polyethylene plasticizer) under ultrasonic radiation at 100 kHz; during subsequent pneumatic extrusion processing of granulated composition, pneumo-dispersive viscous-flow fibers container herein are additionally exposed to ultrasonic irradiation at 250 kHz at a distance 5-10 cm from spinneret and to magnetic field (1400 kA/m) at 10-20 cm from spinneret. The method ensures fine dispersion of ferrite particles in polyethylene-compatible low-viscous medium, facilitates homogeneous distribution of filling material in granules during extrusion beading and further pneumatic extrusion forming of fibrous material, improves deaggregation of ferrite particles, and provides ordered distribution of small particles within fibers.

EFFECT: increased remanence of fibrous composite.

1 tbl

FIELD: chemical industry; other industries; methods of manufacture of the filtering medium.

SUBSTANCE: the group of inventions is pertaining to the field of the water treatment, to be exact to the manufacture process of the polymers of the space-globular structure (SGS polymers), used in the capacity of the filtering mediums. The method of manufacture of the filtering medium consists, that preliminarily exercise thiocyanation of resorcinol, then conduct the joint polycondensation of the resorcinol, the formaldehyde and the product of thiocyanation of the resorcinol. The filtering medium is characterized by the fact, that it contains the product of the joint condensation of the formaldehyde, resorcinol and the product of the reaction of thiocyanation of the resorcinol, and also that it has space- thiocarbomate functional groups-S-C (O)-NR2 defined in the infra-red spectrum of absorption of the polymer sample by the bands with the minimums of 1163 cm-1 (the reference band), 1720 cm-1 (the reference band) and 615 cm-1 (the ill-defined band).

EFFECT: the group of the inventions presents the method of manufacture of the polymers of the space-globular structure used in the capacity of the filtering mediums.

8 cl, 2 tbl, 4 dwg, 6 ex

FIELD: mechanical engineering; filtering materials.

SUBSTANCE: group of invention relates to production of filtering materials featuring high absorbing and filtering properties, namely, to production of filtering materials on base of synthetic polymeric fibers for cleaning and decontamination of water, water solutions and other liquids. Proposed filtering material contains, as base, a polymeric fibrous material obtained by electroforming modified with particles of aluminum hydroxide attached to surface of base fibers to improved sorption properties and forming positive electric charge on material. Said filtering material is formed by modification of polymeric fibrous base received by method of electroforming. For this purpose initial aluminum-based material is applied to polymeric fibrous material and then hydrolysis of initial material is carried out, as a result of which particles of aluminum hydroxide are formed and attached to polymeric fibrous material.

EFFECT: provision of filtering material of low hydrodynamic resistance and high efficiency of filtration.

20 cl, 4 ex, 4 tbl, 1 dwg

FIELD: medical engineering.

SUBSTANCE: device has cover, casing having bottom, aerosol-protection filter and absorbing unit manufactured from beaded sorbent coalesced with plastic. The absorbing unit has mixture of beaded sorbent and polyethylene taken in following proportions (% by mass): carbon sorbent containing 24.0-28.0% of nickel sulfate - 31.0-32.0; carbon sorbent containing 6.0-7.5% of copper, 2.0-2.5% of chromium and 0.04-0.06% of silver - 11.0-11.5; active carbon - 19.0-20.0; active alumina containing 8.0-9.0% of potassium permanganate - 26.0-27.0; polyethylene 11.0-11.5.

EFFECT: enabled general protection against wide greater number of harmful substances having different physical and chemical properties; increased service life.

1 tbl

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