Method of production of vaccine for animal necrobacteriosis prevention and treatment, vaccine for animal necrobacteriosis prevention and treatment and method of animal necrobacteriosis prevention and treatment
SUBSTANCE: group of inventions concerns veterinary microbiology and immunology. Method of vaccine production includes cultivation of strain B.necrophomm "0-1" "ВИЭВ" on nutrient medium, inactivation with formalin, biomass isolation from culture fluid by centrifugation, centrifugate isolation and exotoxin ultrafiltration concentration to protein content 5.5-6.0 mg/ml (by Lowry). Exotoxin B.necrophomm of molecular weight 18000-20000 D is produced. It is added with formalin solution to end concentration 0.4% and inactivated within 15 days. Formalin inactivated exotoxin is mixed with oily adjuvant at the following component proportions (mass%): formalin inactivated exotoxin B.necrophorum - 60.0-67.0, oily adjuvant - 33.0-40.0. Then produced suspension is homogenised and end vaccine is packed up. A vaccine is injected intradermally in single or twice dosage 0.2-0.4 ml. Invention enables to simplify method of production of highly effective vaccine, to produce vaccine of reactogenity, as well as to lower vaccination dose and increase convenience of introduction.
EFFECT: development of effective method of necrobacteriosis vaccination in veterinary science.
10 cl, 4 ex
The invention relates to the field of veterinary Microbiology and immunology, and can be used for the prevention and treatment of microbacteria animals.
There is a method of making a vaccine for the prevention and treatment of microbacteria, including the allocation of local hearth epizootic cultures of microbacteria, its cultivation, inactivation, the separation of the biomass from the culture fluid by centrifugation, extraction of antigen from biomass by three consecutive freeze-thawing of cells and their homogenization followed by centrifugation and isolation of the supernatant protective antigen, representing the cytoplasmic fraction containing endotoxin, and parallel to this, the selection of exotoxin deposition of gelatin, the Association received antigens in the ratio of 1:3, re-adding inactivator formalin and connection with oil-lanolin adjuvant in a 1:1 ratio. Get the vaccine of the following composition: cytoplasmic fraction containing endo - and exotoxin, taken in the ratio 1:3 - 450-550 ml, formalin - 3,5-5,0 ml mineral oil - 381-497 ml, ml to 1 L. the Vaccine lead subcutaneously once, at a dose of 1-2 ml (see, for example, RU 2043773 C1, 20.09.1995).
The known method involves a preliminary selection of local strains for each of concr the private sector, it is impossible in industrial production. In addition, ethnologica phase separation of the bacterial suspension and the concentration of exotoxin and subcutaneous method of vaccine administration is very time consuming.
There is a method of making a vaccine for the prevention and treatment of microbacteria, including the use of strain B.necrophorum VIEW selected on the basis of the maximum immunogenicity and antigenic activity after cultivation which separate the biomass from the culture fluid by centrifugation, the separated biomass resuspended in physiological solution and the resulting suspension extracted endotoxin repeated freezing and thawing to the complete destruction of bacterial cells and separation from cellular detrita by centrifugation, and the culture fluid secrete an exotoxin, then mix the resulting endotoxin and exotoxin in equal volume ratios and add adjuvant based on mineral oil and lanolin. The resulting vaccine is injected subcutaneously twice at a dose of 2 ml, and then at a dose of 3 ml (see, for example, RU 2109519 C1, 27.04.1998).
There is a method of making a vaccine also ethnological and time-consuming, and two subcutaneous administration of the vaccine is also time-consuming and leads to the formation of granulomas at the injection site.
The known method projectors, the manufacture of a vaccine for the prevention and treatment of microbacteria, including the use of the modified strain F.necrophorum, after cultivation which secrete an exotoxin, and receive three types of exotoxin: "inactivated" (with the addition of formalin), "raw" and "paleoceanic", which is used as vaccines (see, for example, US 5455034 And 03.10.1995). The vaccine is also administered subcutaneously twice at a dose of 4.6-20 ml.
The known method is very time-consuming and laborious used doses are very high.
The present invention is to develop a method that allows to obtain highly immunogenic harmless vaccine, which you can enter the animal intradermally, and thus to reduce the dose of an administered drug.
The problem is solved by a method for manufacturing vaccines for the prevention and treatment of microbacteria includes the cultivation of the strain B.necrophorum "0-1" VIEW on a nutrient medium, inaktivirovanie formalin, the separation of the biomass from the culture fluid by centrifugation, separation of centrifugate and concentrated of exotoxin by ultrafiltration to a protein content of 5.5-6.0 mg/ml (Lowry), obtaining exotoxin with a molecular mass of 18000-20000 D., adding formalin to a final concentration of 0.4%, inaktivirovanie received exotoxin B.necrophorum within 15 days, mixing inactivated by formalin exotoxin with oil is a valuable adjuvant in the following components (in wt.%):
|Inactivated by formalin exotoxin B.necrophorum||60,0-67,0|
the homogenization of the resulting suspension and packing of the finished vaccine.
The problem is solved also due to the fact that in the process of cultivation B.necrophorum through the medium periodically miss purified nitrogen concentration of exotoxin carry on ultrafiltration plant with hollow fibers having a pore size of 1.5 kDa, and after mixing inactivated by formalin exotoxin B.necrophorum with oil adjuvant homogenization resulting suspension is carried out on colloidal mill.
The problem is solved and due to the fact that is manufactured by the above method, the vaccine contains (in wt.%):
|Inactivated by formalin exotoxin B.necrophorum||60,0-67,0|
Suitable as an oil adjuvant use a light mineral oil or mineral oil HMD-6M.
The problem is solved also due to the fact that received the vaccine administered to the animals one, twice intradermally at a dose of 0.2-0.4 ml
It is expedient to introduce is accino using without needle injector. As without needle injector is advisable to use a injector B-7 or B-7M.
Suitable for the prevention and treatment of cattle in disadvantaged by nitrobacteria agriculture to administer the vaccine twice with an interval of 4-6 weeks and then once every 6 months at a dose of 0.4 ml
It is advisable to prevent microbacteria cattle to introduce the vaccine in conjunction with hyperimmune serum.
For the prevention and treatment of microbacteria deer it is expedient to introduce the vaccine intradermally a single dose of 0.2 ml.
The technical result of the claimed invention is a simplified method of manufacturing a highly effective vaccine with low reactogenicity, and reduced vaccination doses of the vaccine with increasing facilities of its introduction.
The invention is illustrated by the following examples.
Strain B.necrophorum "0-1" VIEW cultivated on medium Kitt-Tarozzi in bottles for 2 days at a temperature of 37°With, then subcultured into vials with the same environment. Grown macrovu sowing the seed is obtained in the bioreactor with a pre-sterilized nutrient medium, made on the basis of parivara of Hottinger, and cultured for 24-36 hours at 37°C. In the process of cultivation through Wednesday periodically miss purified nitrogen. OK what nanii growing culture checked for purity and optical concentration. Inactivate formalin to a final concentration of 0.4%. Grown inactivated culture is divided into biomass and the culture fluid by centrifugation. From the culture fluid is isolated and concentrated exotoxin on ultra-filtration plant with hollow fibers having a pore size of 1.5 kDa to protein content of 6.0 mg/ml (Lowry), then add formalin to a final concentration of 0.4% and incubated at 42°C for 15 days. Get exotoxin with a molecular mass of 18000-20000 Etc. For the manufacture of vaccines received an inactivated exotoxin mixed with oil adjuvant in the following ratio of components (wt.%): inactivated exotoxin - 60,0-67,0, oil adjuvant - 33,0-40,0. Then the vaccine is homogenized in a colloid mill and Packed. As oil adjuvant use a light mineral oil or mineral oil HMD-6M.
The vaccine obtained in example 1 and having the following composition (in wt.%) inactivated exotoxin - 65,0, oil adjuvant (light mineral oil) - 35,0 use in disadvantaged by nitrobacteria sector for the prevention and treatment of cattle.
The vaccine is administered twice with an interval of 5 weeks, then once every 6 months. at a dose of 0.4 ml intradermally using a needleless injector B-7. Su is th was vaccinated about 100 thousand sick and apparently healthy cattle in 25 regions of the Russian Federation.
Vaccine efficacy was 98±0,2%. The vaccine was no inflammation. The vaccine induces immunity within 6 months after vaccination.
To prevent microbacteria cattle injected hyperimmune serum, and then the vaccine obtained in example 1 and having the following composition: inactivated exotoxin - 63,0 wt.%, oil mineral oil adjuvant HMD-6M is 37.0 wt.%. The vaccine is administered twice with an interval of 6 weeks and then once every 6 months at a dose of 0.3 ml intradermally using a needleless injector B-7.
Inspection conducted on the farms of the Moscow region, showed that the vaccine together with hyperimmune serum helps eradicate the disease within 1-1,5 years.
Obtained in example 1 vaccine and having the following composition: inactivated exotoxin - 60,0 wt.%, oil adjuvant (light mineral oil) - 40.0 wt.% used for the prevention and treatment of microbacteria reindeer. Extensive production and testing of vaccines was conducted in deer farms of the Republic of Sakha (Yakutia). Was vaccinated about 110 thousand deer, incidence after vaccination was 1±0.4 percent. The vaccine was administered intradermally once using batigol the CSOs injector BI-7M dose of 0.2 ml.
The tests showed that the vaccine viacommunity, harmless, protect against disease microbacterium 98-99% vaccinated deer and creates immunity duration of 6 months.
Thus, the claimed invention allows to simplify the method of manufacturing a highly effective vaccine against microbacteria animals receiving the vaccine with low reactogenicity, and reduce vaccination vaccine dose and increase the convenience of its introduction.
1. A method of manufacturing a vaccine for the prevention and treatment of microbacteria animals, including cultivation of the strain B.necrophorum "0-1" VIEW on a nutrient medium, inaktivirovanie formalin, the separation of the biomass from the culture fluid by centrifugation, separation of centrifugate and concentration of exotoxin by ultrafiltration to a protein content of 5.5-6.0 mg/ml (Lowry), obtaining exotoxin B.necrophorum with a molecular mass of 18000-20000 D., adding formalin to a final concentration of 0.4%, inaktivirovanie received exotoxin within 15 days, mixing inactivated by formalin exotoxin with oil adjuvant at the following content, wt.%: inactivated by formalin exotoxin B.necrophorum - 60,0-67,0; oil adjuvant 33,0-40,0, the homogenization of the resulting suspension and packing of the finished vaccine.
2. The method according to claim 1, characterized in that h is in the process of cultivation B.necrophorum through the medium periodically miss purified nitrogen, the concentration of exotoxin carry on ultrafiltration plant with hollow fibers having a pore size of 1.5 KDa, and after mixing inactivated by formalin exotoxin B.necrophorum with oil adjuvant homogenization resulting suspension is carried out on colloidal mill.
3. Vaccine for the prevention and treatment of microbacteria animals produced by the method according to claim 1, containing, in wt.%: inactivated by formalin exotoxin B.necrophorum - 60,0-67,0; oil adjuvant 33,0-40,0.
4. The vaccine according to claim 3, characterized in that as an oil adjuvant contains a light mineral oil or mineral oil HMD-6M.
5. Method for the prevention and treatment of microbacteria animals, including the introduction of a vaccine, characterized in that the animals injected intradermally vaccine according to any one of p and 4 one - twice in a dose of 0.2-0.4 ml
6. The method according to claim 5, characterized in that the vaccine is injected with without needle injector.
7. The method according to claim 6, characterized in that as without needle injector use injector B-7 or B-7M.
8. The method according to claim 5, characterized in that for the prevention and treatment of cattle in disadvantaged by nitrobacteria sector vaccine administered intradermally at a dose of 0.4 ml twice with an interval of 4-6 weeks and then once every 6 months.
9. The method according to claim 5, characterized the eat, what's to prevent microbacteria cattle vaccine is administered in conjunction with hyperimmune serum.
10. The method according to claim 5, characterized in that for the prevention and treatment of microbacteria deer vaccine administered intradermally a single dose of 0.2 ml.
SUBSTANCE: invention refers to methods and compositions for treatment and/or prevention maintenance of infectious condition in liquid-containing organ or organ with natural external aperture. Composition includes antibacterial agent, and also, probably, other active agents, amphipathic oil which is soluble in water and insoluble in ethanol, microcrystalline wax and pharmaceutically acceptable nonaqueous carrier. The method of treatment is realised by introduction of a pharmaceutical composition in body through natural external aperture.
EFFECT: regarding composition, invention provides stability of the active agent against oxidising decomposition, low interfacial tension of composition, easy solubility in liquids, and short time of excretion; invention provides effective treatment and reduction of by-effects and toxicity.
61 cl, 12 ex
SUBSTANCE: can be used as method of treatment of osteomyelitis and septic arthritis, caused by bacterial agents, including with plural medicinal resistance. For this purpose mammal is introduced with pharmacologically effective amount of tigecycline separately or in combination with ryphampycine antibiotic. This agent is also offered also for manufacturing of medical products for treatment of given diseases.
EFFECT: invention provides increased efficiency of bone, marrow and joints infection treatment to preferential tigecycline distribution through bone tissue, marrow and synovial fluid.
48 cl, 5 dwg, 11 tbl, 3 ex
SUBSTANCE: invention relates to the N" -substituted 9a-N-(N'- carbamoyl-γ-aminopropyl) and 9a-M-(M'-thiocarbamoyl-γ-aminopropyl)) derivatives of 9-desoxo-9-dihydro-9a-ase-9a-homoerythromycine A or 5-O-desosaminyl-9-desoxo-9-dihydro-9a-ase-9a-homoerythronolide A, novel semisynthetic macrolide antibiotics from the group of azalides with the common formula 1, where R means H or cladinosyl fragment, R means H, R means the isopropyl group, 1-naphtyl, 2-naphtyl, benzene, 2-(trifluoromethyl)phenyl, 3-phenylpropyl, (3-phenylethyl, ehtoxicarbonylmethyl, 1-(1-naphtyl)ethyl, 3,4,5-trimethoxiphenyl and 2,4-dichlorphenyl, and X means O or S, or to their pharmaceutically compliant additive salts with the inorganic or organic acids and to the method of their production. The invention also relates to the pharmaceutical composition with the antibacterial activity and to the application of the compounds with the common formula 1 or their pharmaceutically compliant salts, in producing the said composition.
EFFECT: antibacterial activity.
16 cl, 26 ex, 1 tbl
FIELD: medicine; pharmacology.
SUBSTANCE: ointment includes vegetable raw materials: sprouts and buds of poplar, aspen or willow, swallowwort (herb and blossom), common wormwood herb, yellow melilot (herb and blossom), costmary (herb and blossom), silver fir (sprouts), Siberian cedar (sprouts), pine resin and fat base, vegetable raw materials ratio is 1:10. As fat base composition contains unsalted pork fat.
EFFECT: extended range of preventive and therapeutic vegetable-based medicinal agents providing effective treatment of inflammatory diseases.
2 cl, 3 ex
FIELD: medicine; veterinary science.
SUBSTANCE: medicinal agent is spirit tincture made on basis of mixed Scotch pine buds, purple Echinacea herb and blossom clusters, horseheal rhizomes and roots, taken in proportion 2:1:1 with 70% concentrated ethyl alcohol 70% at raw and extractant ratio 1:5. Spirit tincture is exposed within 7 days in dark place at temperature 18-20°C. Calves ate treated within three courses every 48 hours with medicinal agent as 3.5-5.0% aqueous solution taken 2.0-3.0 ml/m3 during 45-60 minutes.
EFFECT: provided immunocorrection of key immunity disorders and increased natural resistivity of calves.
2 cl, 4 tbl, 6 ex
FIELD: medicine; pharmacology.
SUBSTANCE: invention is medicinal agent containing rybafutin, copolymer of lactic and glycolic acids (PLGA 50/50), D-mannitol, polysorbate 80 and dimethyl sulfoxide, as well as related method of production implying that mixed rybafutin, PLGA 50/50, D- mannitol, polysorbate 80 are heated at 50÷60°C and stirred until solid phase is completely dissolved, then cooled to room temperature, added with water at ratio 1 : 20 to 1 : 10 followed with nanoparticles˜200÷400 nm suspension produced as a result.
EFFECT: reduced toxicity of active substance accompanying maintained antimicrobic action and simplified production of end formulation.
3 cl, 4 ex, 4 tbl, 1 dwg
FIELD: medicine; pharmacology.
SUBSTANCE: agent contains antibacterial substance of fluoroquinolones, hydrophilic base and optionally at least one adjuvant. As hydrophilic base agent contains silicone glycerohydrogel of composition Si(C3H7O3)4 · xC3H8O3 · yH2O, where 3 ≤ x ≤ 10, 20 ≤ y ≤40. As antibacterial substance of fluoroquinolones agent contains perphloxacyn, ophloxacyn, cyprophloxacyn, norphloxacyn, lomephloxacyn, moxyphloxacyn, csparphloxacyn or enoxacyn. Agent can contain as adjuvant chlorhexidine bigluconate, lydocaine hydrochloride, sea-buckthorn oil, brier oil or mixture. New agent for treatment of suppurative-inflammatory skin and soft tissues diseases including decubitus ulcers, burns, trophic ulcer, has high antibacterial effect due to wide antibacterial action (fluoroquinolones) and high transcutaneous and anti-edematous activity (silicone glycerohydrogel), and do not carry negative by-effects.
EFFECT: agent is stable, easy for keeping and well wipe-off.
6 cl, 5 dwg, 3 tbl, 6 ex
FIELD: medicine; pharmaceutics.
SUBSTANCE: pharmaceutical formulations contains as reactants 1) betamethasone dipropionate or 2) betamethasone dipropionate combined with gentamicin sulphate or 3) betamethasone dipropionate combined with gentamicin sulphate and clotrimazole. Pharmaceutical formulations contain ointment hydrophobic base containing white petrolatum and target additives - preserving agent and hydrophobic nonaqueous solvent. New pharmaceutical formulations have high antibacterial preserving action, high microbiological purity value and potentised antibacterial specific action showed by produced therapeutic effect and simultaneous prevention of by-effect local complication caused by activation of bacterial and mycotic infections.
EFFECT: prevention of by-effect local complication caused by activation of bacterial and mycotic infections.
18 cl, 11 tbl, 6 ex
FIELD: technological processes.
SUBSTANCE: protein variants are suggested that possess lysozyme activity. Protein-based antibacterial substance is described. DNA molecule is discovered that codes the specified protein, and also procariotic cell is discovered that contained the specified DNA molecule. Method is described to prepare protein with the help of procariotic cell.
EFFECT: simplifies preparation of antibacterial substance in commercially significant volumes.
13 cl, 3 ex
SUBSTANCE: invention relates to new derivatives of N-form hydroxylamine of the formula (I) where X means -CH2-, -CH(OH)-, -CH(OR)-,-CF2- or -CH(F) - where R meansC1-C7alkyl, R1 means phenyl, quinoline, izo quinoline, pyridyl oxypyridyl, unessentially replaced by substitutesR6, R7, R8, R9, or means a structural fragment of the formula where each of R10 and R11 independently means H, halogen; everyoneR2, R3, R4, R5 independently means H, C1-C7alkyl, n is equal to 0-2 provided that if n is equal to 0 Xmeans-CH2 everyone of R6, R7, R8, R9 H, OH independently means, halogen, C1-C7alkyl, replaced by halogen C1-C7alkyl, C1-C7alkoxy, phenyl or its pharmaceutically acceptable salt.
EFFECT: compounds display high anti-bacteria activity.
12 cl, 1 tbl, 50 ex
SUBSTANCE: escherichia coli cells containing adhesive gene K 88 ab, K 88 ac, K 88 ad, K 99, F-41, Att-25, 987P are cultivated in liquid nutrient medium to produce vaccine. Physical, chemical and biological indicators are investigated in the process of bacterial bulk growth. In addition, activity of fimbrial adhesin in passive hemagglutination of cellular bacterial bulk is determined. Further cultivation is terminated when fimbrial adhesins titers values are K 88 ab, K 88 ac, K 88 ad, K99, F-41, Att-25, 987P equal to: 1:2048-4096; 1:1024-2048; 1:256-512; 1:1024-2056; 1:1024-2056; 1:32-64; 1:256-512, accordingly. Biological bulk is then concentrated and spinned at 3000-5000 rev/min for 20-25 minutes to separate culture broth from cellular bacterial bulk. Then vaccine is inactivate.
EFFECT: improvement of target product quality.
2 tbl, 5 ex
FIELD: medicine, immunology, in particular method for prophylaxis of post-vaccinal complications in infants.
SUBSTANCE: claimed method includes determination of immunoglobulin A level in infants of one or more years old, and if said level is less than 0.25 g/l vaccination is carried out and ribomunil as administered daily for 12 days.
EFFECT: decreased complication frequency in postvaccinal period.
3 ex, 1 tbl
FIELD: biotechnology, medicine, allergology.
SUBSTANCE: invention proposes a transformed cell of lactobacillus microorganism. Cell expresses surface polypeptide that comprises peptide sequence mimicking part of conformational epitope of IgE molecule. Surface polypeptide is represented by protease from L. bulgaricus anchored on cellular surface. Also, invention proposes a nutrient composition and pharmaceutical composition containing such cell. Proposed invention can be used in treatment of allergy and/or prophylaxis of symptoms of allergic responses.
EFFECT: valuable medicinal properties of microorganism.
11 cl, 3 dwg, 6 ex
FIELD: biotechnology, immunology, genetic engineering.
SUBSTANCE: invention relates to a variant of protein subtilysine from Bacillus amyloliquiefaciens with change Y217L and comprising T-cellular epitope. This T-cellular epitope of indicated variant comprises one or some amino acid changes chosen from group consisting of residues corresponding to positions: 76, 79 and 122 wherein indicated subtilysine variant has optionally change at one or some following positions: 3, 31, 40, 41, 46, 47, 48, 50, 76, 101, 104, 107, 111, 128, 147, 154, 181, 182, 183, 185, 206, 215, 218, 238, 247, 248, 250, 254, 258 and 262. Also, invention relates to DNA molecules that encode subtilysine variants, host-cells containing this DNA, and to compositions used in skin care and containing indicated subtilysine variants. Invention provides preparing subtilysine variants that elicit reduced immunogenic response in human as compared with the parent subtilysine.
EFFECT: improved and valuable immune properties of protein, improved preparing methods.
31 cl, 25 dwg, 3 tbl, 14 ex
FIELD: medicine, vaccines, infections.
SUBSTANCE: invention relates to creature of preparations of bacterial origin. Vaccine strain Nik-sp. F. tularensis is prepared by selection by microbiological method from pure line of bacteria of R-form of the strain 15 NIIEG Francisella tularensis. The strain represents S-form with maximal immunizing dose for laboratory animals from 1 x 104 to 1 x 108 microbial cells in subcutaneous administration and it is able to protect their against infection with Francisella tularensis subsp. tularensis 503. The strain is deposited in All-Russian collection of industrial microorganisms FGUP GosNIIGenetics at number VKPM B-9311. The strain shows low reactogenicity, high stability, possibility in using standard media and technologies in culturing, storage and preparing that allows its using as live tularemia vaccine instead the existing vaccine 15 NIIEG F. tularensis. Invention can be used in public health, medicine and veterinary science.
EFFECT: improved and valuable properties of vaccine.
2 tbl, 4 ex
FIELD: pediatrics, otolaryngology, and infection diseases.
SUBSTANCE: treatment comprises determining level of secretory immunoglobulins sIgG and sIgA in saliva before administration of immunomodulator "IRS 19" and 7 days after administration. In case of increase of sIgA against reduction of sIgG, antibacterial therapy is not prescribed. If increase of sIgG is observed against reduction of sIgA or no changes in the latter, wide action spectrum antibiotics are prescribed in age-specific doses and in generally accepted administration schemes.
EFFECT: achieved long-term stable remission with minimum side effects.
2 tbl, 2 ex
SUBSTANCE: method involves carrying out morphological investigations using morphometric evaluation of preparation quality by its harmless and effectiveness of topical protective response based on state of three groups of cellular fractions of intestine mucosa comprising interepithelial lymphocytes, apudocytes and goblet-shaped cells. Material is sampled from three compartments of intestine: duodenum, ileum and ascending region of colon. Obtained quantitative data are subjected for mathematical treatment using developed coefficients and indices calculated by formulae. Advantage of invention involves expanding assortment of evaluating criteria. Invention is directed for enhancing evaluation of quality of vaccine preparations used against cholera.
EFFECT: improved and valuable evaluating method.
4 tbl, 2 ex
FIELD: biotechnology, pisciculture.
SUBSTANCE: the innovation deals with preparing nutritive media, inoculation material and obtaining sensitin out of the strains Vibrio anguillarum N2 and VUR-19 followed by sensitization of ram's erythrocytes. Moreover, it is necessary to apply 20%-suspension of formalinized erythrocytes upon phosphate-buffer solution at pH being 7.0-7.4. Sensitization should be carried out upon water bath in two stages. Erythrocytes and sensitin should be taken at the ratio of 1:0.3-1:0.35.
EFFECT: higher efficiency.
FIELD: medicine, pediatrics, otolaryngology, endocrinology.
SUBSTANCE: the present innovation deals with preventing acute respiratory diseases of the upper respiratory tract in children at insulin-dependent diabetes mellitus during the period of compensation and subcompensation. Before vaccination it is necessary to determine the concentration of antibodies to total antigens of "Pneumo 23" vaccine in blood serum and detect the coefficient of balance capacity (Cbc) in saliva. At the titer of total antibodies to total antigens of "Pneumo 23" vaccine being below 30 CU/ml and Cbc being above 2.1 one should be fulfill vaccination due to single introduction of "Pneumo 23" vaccine into area of deltoid muscle at the dosage of 0.5 ml simultaneously with immunomodulator "IRS-19" introduced per a dose for each nasal meatus twice daily for 14 d including the day of vaccination. The innovation suggested provides the stable maintenance of a stable high titer of antibodies to antigens of "Pneumo 23" vaccine, higher lysozyme activity and secretory immunoglobulin A and prevents acute respiratory diseases in postvaccinal period.
EFFECT: higher accuracy and efficiency of prophylaxis.
3 ex, 1 tbl
FIELD: molecular biology, veterinary.
SUBSTANCE: invention proposes isolated DNA sequence (variants) encoding Ehrlichia canis protein of size 30 kDa. Also, invention proposes vector comprising such sequence, recombinant Ehrlichia canis 28 kDa protein encoded by this sequence, a cell-host comprising this sequence, a method for preparing the protein, immunoreactive antibody specific to this protein and a method for inhibition of Ehrlichia canis infection in subject. Recombinant protein of size 28 kDa from Ehrlichia canis shows immune reactivity with respect to serum against Ehrlichia canis. Proposed group of inventions can be used in development of vaccines and serodiagnosticum that shows high effectiveness for prophylaxis of diseases and for carrying out the serodiagnosis.
EFFECT: improved preparing method, valuable medicinal and veterinary properties of protein.
19 cl, 17 dwg, 8 ex
FIELD: biotechnology, vaccines.
SUBSTANCE: vaccine comprises bacterial mass of Pasteurella multocida of serovariants A, B and D, Haemophilus pleuropneumonia of serogroups 1 and 2, and streptococcus of serogroups C and R, and also lysate-anigens of salmonellae Salmonella cholerae - suis, strain № 370 and Salmonella typhimurium № 415 mixed in the definite concentration. Vaccine elicits the high immunogenicity and provides the protection of pigs against infectious pneumonia of bacterial etiology and salmonellosis.
EFFECT: valuable veterinary properties of vaccine.
3 cl, 1 tbl, 5 ex