Method of simultaneous verification of uveal melanoma course forecast
FIELD: medicine; ophthalmology.
SUBSTANCE: invention is intended for simultaneous verification of uveal melanoma and forecast of metastasis development. Immunohistochemical analysis of protein S-100 and melanin-A expression within tumour cell is accompanied with simultaneous estimation of S-100-positive cells number within visual field. Uveal melanoma is verified if reaction of S-100 and melanin-A is positive with forecasted high possibility of tumour dissemination in case number of S-100-positive cells number is less than 50.
EFFECT: simplified examination associated with high specificity and sensibility of simultaneously verified uveal melanoma and forecasted metastasis.
1 tbl, 1 dwg, 2 ex
The invention relates to ophthalmology, namely oftalmologii, and is intended for simultaneous diagnosis and prognosis of metastasis of uveal melanoma (UM).
According to the modern requirements to the diagnosis of human cancers each tumor must be confirmed not only by light or histological level, but also on immunohistochemical tissues. Nowadays, the histologists have a wide range timedifference markers by which conduct verification of tumors. The term "verification" means the determination of its tissue of origin or histogenesis.
For verification of MIND, as well as melanoma of the skin, use a few standard melanomamelanoma markers. These include: S-100, tyrosinase, melanin-And NIV-45, MITF [Guide immunohistochemical diagnosis of tumors of the human - edited Svitava and Nutricline. - Kazan. - 2000 - Str]. The frequency of detection of these markers in MIND, as well as the number of positive tumor cells for these markers in the tumor, according to the literature, varies considerably. This means that the sensitivity of different melanomamelanoma markers for verification of melanomas in different locations varies significantly. In other words, according to some authors, there may be situations when this is the markers do not recognize melanoma, the reaction of this marker will be a false-negative [Beckenkamp G., Schafer H., von Domarus D. Immunocytochemical parameters in ocular malignant melanoma.// Eur j Cancer Clin Oncol, 1988, vol.24, p.41-45; Bumier M.N. Jr., I.W. McLean, Gamel J.W. Immunohistochemical evaluation of uveal melanocytic tumors.// Cancer, 1991, vol.68, p.809-14; Femando S.S., Johnson, S., Bate J. Immunohistochemical analysis of cutaneous malignant melanoma: comparison of S-100 protein, NIV-45 monoclonal antibody and NKI-C3 monoclonal antibody. // Pathology, 1994, vol.26, p.16-19. Heegard, S., Jensen O.A., Prause J.U. Immunohistochemical diagnosis of malignant melanoma of the conjunctiva and uvea: comparison of the novel antibody against melan-A with S-100 protein and HMB-45.// Melanoma Res, 2000, vol.10, p.350-354.]
Therefore, to clarify the situation and to improve the quality of tissues diagnosis, we tried to find the most reliable and accurate markers for verification of these tumors among other intraocular neoplasm. For this we have taken from the archive of our Institute paraffin blocks of tissue MIND, obtained from 82 patients operated for more than 5 years ago, and conducted its own tissues-study. The purpose of these studies was to evaluate the reliability of the use of any marker in the diagnosis or, in other words, the evaluation of their sensitivity. This could be done by the number of false negative cases. To assess the specificity of the markers we also took other intraocular tumors - leiomyosarcoma and lymphoma. The specificity of tissues markers, generally analysed to identify false positive reactions melanocytic markers opuholyah kamelancien series. Along the way we conducted a retrospective analysis of the results of the tissues studies in correlation with clinical data regarding the survival of patients.
It turned out that almost all markers in the majority of cases (94,9-100%) give a positive reaction with S-100, tyrosinase, melanin-A, HMB-45 and MITF. However, the sensitivity of different melanomamelanoma markers really is different. In fairness, we note that this difference was much lower than what was described by these authors (see table 1). The results of this research showed that using a single S-100 cannot with absolute conviction to assert that S-100-positive tumor is melanoma. This marker gave a positive response and tumor myogenic series - leiomyosarcoma. That is talking about the fact that this marker is not highly specific, such as the melanocyte lineage markers tyrosinase, melanin-A, HMB-45, MITF. It is the latter are highly specific markers and not give false positive results. Therefore, their presence is necessary for verification of melanomas. However, they give false negative results. So, from 82 melanoma in 1 case, the reaction with tyrosinase was negative. In practice, this means that using only one of tyrosinase in tissues diagnosis would lead to diagnostic error. Similar to the context would have been and when using only one of MITF. This marker also gave a false-negative reaction with melanoma. Ideal melanomamelanoma markers were NIV-45 and melanin-A (see table).
As shown by our own research, the use of two markers (S-100 and one of melanomacrophage markers: melanin-a or NIV-45) increases the reliability tissues diagnostics. The advantage of melanin-And before NIV-45 is the fact that melanin-And stain premelanosomes, while NIV-45 - Mature melanosomes, and does not stain tumors of other origins. That is, these two markers are responsible for 2 different stages of maturation of melanosomes. And because different populations of tumor cells, as a rule, are at different stages of maturity and, therefore, at different stages of melanogenesis, the MIND will find melanin-A-positive tumor cells. That, in fact, we have obtained empirically.
Our retrospective analysis also showed that there is a direct close relationship between the expression of S-100 and survival of patients. This fact for some reason was not reflected in the literature, or simply not been noticed by other authors. It turned out that the smaller the tumor is detected S-100-positive cells, the worse the prognosis. Statistical retrospective analysis showed that the critical number of S-100-positive TC is the current forecast 50. That is, in other words, the detection in tumors less than 50 S-100-positive cells is the probability of developing metastasis was high and reached 82.2 per cent. As proof here is a graph of the survival of patients with two diametrically opposite indicators in the tumor. Thus, according to this schedule (see drawing), among 28 patients with less than 50 S-100-positive cells in the tumor (stratum A), metastases developed in 9 cases (32% of cases), and the frequency of their 5-year survival was 68%, respectively. Among 29 patients with ≥50 S-100-positive cells in the tumor (stratum) metastasis occurred in 5 (17.8 per cent of cases), and the survival rate reached 82.2 per cent. The difference in survival rates reliable. This is confirmed by 3 different tests quality control of the survey.
Therefore, in our subsequent work we are using these two markers for verification purposes, uveal melanoma, and quantitative analysis of S-100-positive cells in tumors has served us as a marker for prediction of metastasis.
The task of the invention is to provide such a method that would allow at the same time not only to verify uveal melanoma, but also to predict the likelihood of its spread.
The technical result is to solve simultaneously the time of the two tasks (verification of the tumor and the prediction of the probability of metastasis) using only two markers (S-100 and melanin-a) and one-step immunohistochemical studies, that provides simplified way with a positive economic effect.
The technical result is achieved by using two timedifference markers S-100 and melanin, responsible for the affiliation of the tumor to neoplasma melanocyte lineage.
Our method of prediction is as follows.
1. After enucleation of eyes with uveal melanoma prepare paraffin sections with a thickness of 3-5 μm on a standard methodology, deparaffinized in xylene and rehydration in the battery spirits.
2. Immunohistochemical study performed by standard methods. To open antigenic determinants spend processing sections in citrate buffer [pH 6.0] for 30 minutes at 95°With water bath.
3. Incubation with primary antibodies spend over night at 4°C. To visualize the reaction of the antigen-antibody used streptavidin test system LSAB+kit [DAKO Corp] according to the instructions. As a Chromogen use DAB+[DAKO Corp]. As the primary antibodies used rabbit polyclonal antibodies to S-100 (dilution 1:500, DAKO Corp) and melanin-a (dilution 1:80, DAKO Corp).
4. Then the slices Domracheva with hematoxylin Mayer and conclude in canadian balsam.
5. The reaction is evaluated using a light microscope (magnification ×400) according to the following criteria. otricatelniy considered reaction in the absence of specific staining of tumor cells in the entire area of the tumor tissue. A positive reaction was assessed by staining of tumor cells. When a positive response of tumor cells with two markers, the tumor will verify as uveal melanoma.
6. Quantitative assessment of expression of the marker S-100 by slice is defined as the number immunopositive cells in the field of view of the microscope at magnification × 400. In the case where the number of S-100-positive cells was less than 50, define a high risk of developing metastases. If the number of S-100 positive cells in the tumor is greater or equal to 50, it is considered that the risk of metastases to the patient is low.
Example 1. The patient 54 years old. Diagnosis: the MIND in stage T3NoMo on his right eye. Conducted conservative treatment in the amount of iridocyclectomy. Immunohistochemistry paraffin slice of the tumor at the specified method showed S-100-positive and melanin-A-positive tumor, which was verified as melanoma. Estimate the number of S-100-positive cells on the cut MIND when zooming in × 400: they were 17. The patient is classified as at high risk of developing metastases. Assigned shorter intervals between visits to the doctor. After 8 months of follow-up after the operation the patient revealed metastases in the liver. Conducted immunotherapy to slow the rate of metastasis.
Example 2. The patient was 72 years. Moved the nucleation of the left eye about the MIND in the stage T4NoMo. Immunohistochemically showed S-100-positive and melanin-A-positive tumor, which was verified as uveal melanoma. Estimate the number of S-100-positive cells. On the cut MIND when zooming in × 400 of them turned out to be 87. The patient is classified as low risk of developing metastases. Continuous observation of the patient during the 3 years showed favorable course of the disease and the absence of metastases.
Thus, the proposed method with a high degree of probability allows to simultaneously perform both verification uveal melanoma and to evaluate the prognosis of its flow, which allows the selection of patients at risk of developing metastases and justifies the tactics of management of patients with this pathology. The use of only two markers allows to reduce the duration of the study and the economic costs of their implementation.
The method of simultaneous verification of uveal melanoma and to predict metastasis, including immunohistochemical analysis of the expression of protein S-100 and melanin-And simultaneously counting the number of S-100-positive tumor cells in field of view, and verification of uveal melanoma with positive reaction for S-100 and melanin-And predicting a high probability of metastasis of a tumor in the presence of men who e 50 S-100-positive cells.
FIELD: medicine, veterinary.
SUBSTANCE: specific antibodies to virus of goose enteritis are determined by IEA where virus received following injection of epizootic strain "P-75" of enteritis virus, is used as an antigen, and macro porous glass with diameter not less than 700 is used for purification of virus-containing material, the goose Ig G specific immunoperoxidase conjugate is used as an anti-specific agent, and the IEA results are calculated by use of the formula: titer = antiIg[2.02(lg S/P)+3.51], where S is for optical density of tested serum; P is for optical density of the positive control.
EFFECT: method reduces the test time and economical costs.
2 tbl, 2 ex
SUBSTANCE: method involves determining total IgA quantity using immunoenzyme method based on polyclonal IgA antibodies as binding antibodies on the micropanel and the same antibodies in conjugate with peroxidase for detecting bound IgA in sample before and after treatment with specific IgA1-protease. Total IgA1 and IgA2 content is determined before being treated with enzyme. IgA2 content only is determined after being treated with enzyme. IgA1 is not determined after being disintegrated with IgA1-protease. Kit has microplate containing absorbed polyclonal IgA antibodies, conjugate of the same antibodies with peroxidase, reference sample having given IgA concentration, IgA1-protease and substrate buffer.
EFFECT: simplified method and kit for determining IgA1 and IgA2; improved results reproducibility; no specific monoclonal antibodies being used.
2 cl, 1 tbl
SUBSTANCE: method involves absorbing myeloma IgA1 on micro panel, incubating solutions containing IgA1-protease and determining enzyme activity from IgA1 sorbate quantity reduction determined by conjugate with polyclonal IgA antibodies peroxidase. Determining IgA1-protease activity with inhibitors available in various concentrations enables one to calculate its inhibition constants. Kit has microplate containing absorbed myeloma IgA1 preparation, peroxidase conjugate with antibodies against human IgA and substrate buffer.
EFFECT: simplified method and kit for determining IgA1-protease activity and studying its inhibition process.
2 cl, 1 dwg, 1tbl
FIELD: veterinary virology.
SUBSTANCE: the present innovation deals with interaction of antibodies with an antigen, with antibodies labeled with horseradish peroxidase, addition of substrate mixture and registration of reaction results. Moreover, one should apply plotting boards with presorbed antibodies and general immunoenzymatic conjugate. The innovation enables to shorten terms for diagnosis and obtain more significant results of diagnostics.
EFFECT: higher efficiency.
FIELD: biotechnology, analytical chemistry.
SUBSTANCE: claimed method includes providing of complexes between antigen molecules and specific antibodies on carrier surface, wherein said complexes are disclosed by addition of enzyme label thereto followed detection thereof based on formation of enzyme reaction product. Enzyme label addition is carried out by two protein interaction, namely bacterial ribonucleaze barnase and barstare, which is inhibitor thereof, wherein either of the two is added to immunoreagent, and the other one is added to enzyme label. Abovementioned complexes have high affinity, specificity, and binding constant of 1014 M-1.
EFFECT: new method for antigen detection.
6 dwg, 2 ex
FIELD: veterinary and medicine.
SUBSTANCE: invention, in particular, relates to production and use of biological preparations intended for differential diagnostics of brucellosis and to a method of differentially diagnosing brucellosis. Method involves serologic analysis of sera using antigenic "IFK", which is horse-radish peroxidase-labeled electrophoretically purified polypeptide fraction of virulent strain B.arborus 54. Diagnosis of brucellosis is stated when anti-brucellosis antibodies in sick animal sera diluted to 1/100 and higher are revealed at CSP reaction intensity 2.1 and higher.
EFFECT: elaborated method increasing immuno-enzymatic test specificity and allowing performing differentiation of postvaccinal immunological reactions and postinfectious reactions induced by microorganisms having antigenic affinity with brucellas, especially Yersinia enteriocolitica.
2 cl, 4 ex
FIELD: medicine, biotechnology.
SUBSTANCE: the present innovation deals with elaborating diagnostic reagents for testing prionic protein in mammalian cerebral tissue due to IEA technique and refers to antibodies specifically reacting with prionic protein PrP or its fragment. The innovation includes polyclonal antibodies obtained to synthetic peptides including amino acid sequences of bovine prionic protein 143-168, 101-134 and 211-241, their conjugates with horseradish peroxidase and diagnostic reagents obtained upon their basis that enable to detect prionic protein in mammalian cerebral tissues.
EFFECT: higher specificity of detection.
15 cl, 8 ex, 8 tbl
FIELD: medicine, obstetrics.
SUBSTANCE: in pregnant women at pregnancy terms being after 39 wk in average portion of morning urine one should detect due to a solid-phase immunoenzymatic assay the concentration of pregnancy-associated protein-A (PAPP-A). At the level of PAPP-A being 768.2 ng/ml and more it is possible to conclude upon physiological mature pregnancy. The innovation is noninvasive and enables to increase accuracy in predicting true over-mature pregnancy.
EFFECT: higher efficiency and accuracy of diagnostics.
2 ex, 1 tbl
SUBSTANCE: one should test blood serumal samples in immunoenzymatic assay by applying a test system ELI-N-1, the components of which are being antigens of nervous tissue or their immunochemical analogs specifically binding antibodies with tendency to antigens of nervous tissue, and, also, idiotypical antibodies to them, or their variable parts. Prediction should be performed according to the level of idiotypical and anti-idiotypical autoantibodies to antigens of nervous tissue and, also, according to their ratio. Thus, a new test system has been elaborated that enables to predict the flow of available nervous-psychic diseases.
EFFECT: higher accuracy of prediction.
2 cl, 7 ex
FIELD: medicine, cardiology.
SUBSTANCE: one should detect the level of activity of IgM and IgG immunoglobulins to cytomegalovirus on the 5th and 15th d of large-focus myocardial infarction. At increased diagnostically valuable result of specific immunoglobulins of type M by 0.10-0.20 times and for type G by 0.73-2.09 times it is possible to predict favorable clinical flow of large-focus myocardial infarction without exudative pericarditis. At the value of specific immunoglobulins exceeding diagnostically valuable result for type M - by 0.86-1.67 times and for type G by 2.42-3.01 times one should predict early clinical flow of post-infarction pericarditis. The method enables to carry out prophylactic measures in due time.
EFFECT: higher accuracy of prediction.
5 ex, 2 tbl
FIELD: medicine; ophthalmology.
SUBSTANCE: anatomical features of eye are measured and coefficient of glaucoma acute exacerbation forecast C is calculated. Coefficient is equal: where: C is coefficient of glaucoma acute exacerbation forecast; AC - anterior chamber deep in mm; CL - crystalline lens thickness in mm; APA - anteroposterior axis in mm. C value less 0.29 indicates that there is no glaucoma acute exacerbation hazard. If C value is from 0.3 to 0.32 developing glaucoma acute exacerbation is forecasted, and preventive treatment is prescribed. C value equal 0.33 and more indicates glaucoma acute exacerbation.
EFFECT: provides forecast of glaucoma acute exacerbation.
FIELD: medicine; immunotherapy.
SUBSTANCE: indications for adjuvant immunotherapy of primary and metastatic malignant unresectable liver tumours are determined. For this purpose within surgical process incision biopsy of tumour and healthy liver tissue is performed. Then immediately after operation lymphocytes of tumour tissue, healthy tissue and peripheral blood by markers CD4+, CD8+, CD16+, CD56+ are immunophenotyped. In case lymphocyte marker levels CD4+, CD8+ and NK-cells levels CD16+ and CD56+ are lowered more than 10% for tumour tissue and peripheral blood in comparison with healthy tissue and peripheral blood, postoperative intraportal prolonged introduction of rhoncoleukin is indicated.
EFFECT: provides accurate direct evaluation of local immunity condition; enables to improve treatment results.
12 dwg, 4 tbl, 1 ex
SUBSTANCE: invention relates to medicine, namely to the endocrinology, oncology and radiotherapy. It is used for the diagnosis and assessment of the adipose tissue percentage in the mammary gland. The lattice is imposed on the mammogram with the squares sized 2 x 2 cm. Depending on the fat content there are identified the groups with its content which equals to 0-10%, > 10 - 25%, > 25-50%, > 50-75%, > 75-90% and > 90%. These groups are assigned the points from 1 to 6. Then the fat content ratio is calculated. The coefficient is a quotient from the sum division by the total number of squares. This method allows improving the accuracy of the adipose tissue percentage evaluation in the mammary glands of the women with postmenopausal and reproductive age on the basis of the analysis of mammograms.
EFFECT: improving the accuracy of the adipose tissue percentage evaluation in the mammary glands of the women with postmenopausal and reproductive age on the basis of the analysis of mammograms.
2 tbl, 3 dwg
SUBSTANCE: invention relates to medicine, namely to the phthisiology and it can be used for differential diagnosis of tuberculosis and spine osteomyelitis. The diagnostic criterion of tuberculosis and spine osteomyelitis is calculated by the formula using five most significant clinical and laboratory coded indicators. Figure 1 is a code for gradual start of the disease, lymphocyte transformation reaction with permanent positive pressure (R) >6%, adenosine deaminase values (A) >19.8 units/l, ELISA analysis indicators (I) > 0.2 units. RP, consumption reaction values of complement with tuberculosis antigen (K) > 17 standard units. Figure 2 is a code for the beginning of acute illness, R < 6%, A < 19.8 units / l, I < 0.2 unit, RP, K < 17 standard units. Ciphers of clinical and laboratory values are included into the diagnostic indicator of tuberculosis and spine osteomyelitis formula: Y1 (TB diagnostic indicator of the spine) = 90.0xO +35.5xR +31.7xA-21.1xI+13.1xK-100,3; Y2 (Diagnostic spine osteomyelitis index) = 139.0xO +50.5xR+45.5xA-32,7xI +19.8xR-220.2. The values Y1 and Y2 are calculated. The maximum diagnostic index "Y" indicates a form of the disease entity the patient suffers from. The method allows to establish the diagnosis more accurately.
EFFECT: increasing of accuracy in tuberculosis and spine osteomyelitis diagnosis.
1 tbl, 2 ex
SUBSTANCE: one performs gastric mucosa bioimpedansometry in the area of greater Z1 and lesser Z2 curvature. One evaluates the ration K, as relation of impedance Z1 to Z2 and, if K≥1.4, than it can be sail about gaster peptic ulcer.
EFFECT: proposed method is simple, objective and allows fail-safe detect the gaster peptic ulcer.
1 ex, 2 tbl
SUBSTANCE: therapeutic medical care system is a set of hardware and software including the computer system with the medical and economical standards generating and editing subsystem, access facilities to the database and facilities which provide implementation of medical care. The system can contain the subsystem of hospital customer-specialist terminals of departments and services and it can be compatible with patient accounting routine and accounting software. This invention provides the data systematization of diagnosis and cure of diseases, the ability to choose the appropriate set of medical service which excludes the reasonless volume prescription of diagnosis and medical care with the cost calculation of the pathology diagnosis and treatment for the patient.
EFFECT: strength increasing of direct care with simultaneous quality increasing of performed medical care without working hours increasing of medical staff.
2 cl, 11 dwg
FIELD: medicine, orthopedics and traumatology.
SUBSTANCE: method can be useful for early diagnostics and prevention of thromboembolism in hip and knee replacement in traumotologic, orthopedic, surgery hospital departments, clinics and research institutes. In preoperational period, antithrombin III is evaluated, sodium thiosulfate 10 ml is injected intravenously daily, at the same time cuff test is taken by blood sampling following tonometer's cuff application and pumping pressure equal to systolic, antithrombin III is evaluated in blood samples and if its level is not changed against the baseline preoperational preparation is taken with sodium thiosulfate injection until antithrombin III is elevated during cuff test. And at that, sodium thiosulfate can be injected during 5-8 days up to the moment when antithrombin III is increased 80% from baseline and positive dynamics in cuff test is maintained.
EFFECT: method increases impartiality and precision of preoperational diagnostics of thromboses and embolism.
3 cl, 3 tbl, 1 ex
SUBSTANCE: method involves preparing biological indicator and recording human pulse radiation. Cover glass is placed over human pulse projection zone which is covered with the biological indicator composed of 0.1% aqueous amino acids solution taken in equal proportions: aspartic acid, alanine, valine, serine, glycine, tyrosine, glutamic acid, threonine, 0.5% aqueous dophamine solution, 0.5% aqueous histamine solution, 12% aqueous magnesia sulfate solution taken in 3:2:1:4 proportion. Glass is kept on pulse 4-5 min long, then the preparation is dried at T=+18-20°C during 2-3 min and examined in polarized light with quartz compensator. Radial-beam and/or lotus-like spherulites being detected in sample, hyper-beta-endorphinia is considered to be the case.
EFFECT: simplified method.
SUBSTANCE: method involves carrying out immunohistochemical examination when applying neoadjuvant treatment, in particular, paratumoral chemotherapy on Autoplasma combined with radiation therapy. When examining treatment results with immunohistochemical methods, melanoma marker Melan PNL2 expression is determined. Non-uniform or moderately marked melanoma marker Melan PNL2 expression being found unchanged with cytospecific melanocyte Melan PNL2 antigen being retained, reaction with apoptosis factor p53, with antibodies bcl-2, with proliferation factor Ki-67 marker and with antibodies CD95 is recorded. If no reaction with apoptosis factor p53 and reaction with antibodies bcl-2 being observed or weak positive antigen staining, low proliferative melanocyte activity, proliferation block as Ki-67 protein localization injury and apoptosis activation in reaction with antibodies CD95 being available, the treatment is estimated as effective, pointing out to tumor pathomorphosis.
EFFECT: wide range of diagnostic applications.
SUBSTANCE: method involves determining polymorph markers of gene candidates. Genetic analysis of sportsman blood is done and predisposition and protection alleles and genotypes of polymorph markers of gene candidates are determined like T174M marker of AGT gene predisposition allele M and protection allele T, A(-153)G marker of AT2R1 gene predisposition genotypes AG, GG and allele G and protection genotype AA and allele A, A1298C marker of MTHFR gene predisposition genotype CC and allele C and protection allele A, C825T marker of GNB3 gene predisposition genotype CC, Ala(-9)Val marker of SOD2 gene predisposition genotype AA and allele A and protection genotype VV and allele V, T(-262)C marker of CAT gene predisposition genotype CC, Cys311Ser marker of PON2 gene predisposition genotype Ser/Ser and protection genotype Cys/Ser, I/D marker of gene ApoB predisposition genotype I/I and allele I and protecting genotypes I/I allele I and protection ID and DD and allele D for cardiac ischemia cases; Ser447Ter marker of LPL gene predisposition allele Ser and protection allele Ter, Pro12Ala marker of PPARG2 gene predisposition allele Ala and protection genotypes Pro/Pro and allele Pro, A1298C marker of MTHFR gene predisposition allele C and protection genotype AA and allele A, T(9282) marker of CAT gene predisposition genotype TC and protection genotype CC, Lys198Asn of END1 gene predisposition genotype Lys/Lys for hypertension disease cases; 4a/4b marker of NOS3 gene predisposition genotype 4b/4a and allele 4a and protection genotype 4b/4b and allele 4b, A(-153)G of AT2R1 gene predisposition genotypes AG and allele G and protection genotype AA and allele A, A1298C marker of MTHFR gene predisposition genotype CC and allele C and protection allele A, G7831A of gene ACE predisposition genotypes AA and GA and allele A and protection genotype GG and allele G for hypertension heart cases. It gives base for making conclusion about predisposition when quantitative predomination of predisposition genotypes and alleles take place. Equal quantities of predisposition and protection genotypes and alleles or quantitative predomination of protection genotypes and alleles being the case, genetic protection against pathologic changes development is concluded to be available.
EFFECT: high reproducibility, reliability and accuracy of complication development prognosis.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl