Composition and methods of care of oral cavity based on morinda citrifolia

FIELD: medicine.

SUBSTANCE: invention relates to the compositions for care of the oral cavity, which contain the components of the Indian Mulberry, known as Morinda citrifolia L., for treatment of single or multiple abnormalities of the oral cavity and dental abnormalities, including the periodontal diseases, such as gingivitis and periodontitis, tooth destruction, bad smell and other disorders and irritations of the oral cavity.

EFFECT: composition provides efficient care of the oral cavity.

36 cl, 20 tbl, 11 ex

 

1. The scope of the invention

The present invention relates to compositions for the care of the oral cavity and, more specifically, to compositions for oral care mouth that contribute to the health of the teeth and chewing gum, including plant components of the Indian mulberry, known in science asMorinda citrifolia L.

2. Background of the invention and the technical field

Teeth are the only body tissue of a mammal, not subject to metabolic turnover, which thus saves them almost no damage. Despite this, the teeth are constantly exposed to bacteria that can cause decalcomanie tooth enamel and erosion of the surrounding tissues of the mouth.

The teeth are solid containing calcium structures attached to the jaw bones of vertebrate animals so that in the first place to carry out the function of mastication. Humans and most other mammals have a temporary set of teeth, changing, or milk teeth; in humans they usually erupt between 6 and 24 months. Their total number is 20: 2 Central incisors, 2 lateral incisors, 2 canines, and 4 small molar teeth in each jaw. At the age of about 6 years temporary teeth begin to fall out as they are replaced by permanent teeth. The last permanent teeth (wisdom teeth) mo is ut not appear until the age of 25 years and some people do not erupt. The number of permanent teeth is usually 32: 4 incisors, 2 canine, 2 premolars and 4 (or 6, if developed wisdom teeth) molar in each jaw. Canines are the smallest found in mammals. In all mammals the tooth consists of a crown, the part that is visible in the mouth, and one or more roots that are attached to deepen the gums. Part of the gums surrounding the root, known as the periodontal membrane, surrounds the root bone in his deepening. The jaw bones are strong pinning for root. The Central part of the crown is filled with soft tissue of the pulp, which contains blood vessels and nerves; this fabric extends to the end of the root canal. Surrounds the pulp and is more solid tooth, bone - dentin. Part of the root is the upper layer of cement of the tooth, whereas the part of the crown has an additional layer of enamel, the hardest substance of the body.

For the development and maintenance of healthy teeth need proper nutrition, especially adequate amounts of calcium, phosphorus and vitamins D and C. the Most common violation, damaging the teeth is dental caries (tooth decay). A widely accepted explanation of the process of tooth decay is that bacteria saliva turn the particles of carbohydrates in the mouth into lactic acid that attacks the enamel, dentin and if not is been treated, the pulp of the tooth. Regular cleaning and checkup at the dentist once every six months are important for preventing tooth decay and gum disease. Fluoridation of water sources for the population and the use of fluoridated toothpastes also help to prevent tooth decay.

Periodontal diseases, including gingivitis, are an infection of the tissues that surround and support the teeth, including the gums, periodontal ligament and alveolar bone. Although several factors can exacerbate periodontal disease, the primary cause of periodontal disease is associated with bacteria contained in plaque.

Plaque is a sticky, colorless film of bacteria and sugars that constantly forms on the teeth. Plaque causes the formation of holes in the teeth, when acid residue affects your teeth after eating, eventually causing the destruction of tooth enamel, leading to destruction of the tooth and the emergence of bad breath. If left untreated, periodontal disease can develop, leading ultimately to tooth loss, as well as contributing to such painful conditions as stroke, diabetes, premature birth, heart disease and respiratory disease.

Microorganisms play a significant role in the development of caries, endodontal and periodontal h the problems which can destroy the tissues of the oral cavity. In the report of 1999 stated that more than 50% of all children, 85% of all adults over the age of 18 years and more than 50% of people older than 75 years suffer from caries. The decay of the active places lesions often must be removed to prevent progressive damage to the remaining parts of the teeth. Remove caries is the main cause of tooth restoration. Secondary caries is also directly responsible for 50% of all unsuccessful cases of recovery. The net effect of caries is that in the United States 90 million restored teeth should be replaced and another 200 million restorations should be done every year. Additionally remove countless millions of teeth, and each year in the United States for 15 million tooth is root canal treatment. Only in the USA, approximately 60 billion dollars is spent annually on professional dental treatment, 172 billion dollars on medical products, 122 billion dollars on drugs and $ 50 billion for other medical products. In the remaining part of the world, according to estimates, over 200 billion dollars have been spent on professional dental treatment.

Identified a large number of species of bacteria that inhabit the mouth. However, because the bacterium is s infections, availability of power and low oxidation potential in the root canals with necrotic pulp number of species of bacteria in endodontic infections is limited. These selective conditions lead to the dominance of anaerobic microorganisms that survive and multiply, causing infection, which stimulate local bone resorption and are more resistant to endodontic treatment. Cleaning is one of the main tasks of preparation of root canals. Thorough cleaning removes microorganisms, allows better adaptation of materials-fillers and enhances the effect of intracanal medications. The choice of leaching agents is of great importance because they act as lubricants during tooling, wash away debris and bacteria from the channel and interact with pulp, necrotic tissue and microorganisms and their byproducts. With this purpose for several decades have been extensively used in sodium hypochlorite. Its excellent properties of tissue dissolution and antimicrobial activity determined his choice as an irrigating agent in the treatment of teeth with pulp necrosis, despite some undesirable characteristics, such as toxicity to tissues at high concentrations, risk of emphysema when s is ipolnenie, allergenicity and unacceptable odour and taste. Moreover, the sodium hypochlorite is not completely cleans the surface of the walls. For a variety of purposes as an alternative to sodium hypochlorite was studied chlorhexidine; including antimicrobial activity and biocompatibility. However, recently it was found that its ability to clean the walls of the root canals of the tooth pulp inferior to sodium hypochlorite. These problems suggest that the sodium hypochlorite and other alternative medicines, including chlorhexidine, not fully optimized; and should evaluate new irrigating agents for use as a dental medicines.

Ideal irrigating agent should possess antimicrobial activity, low toxicity and good biocompatibility in relation to oral tissues and must possess the ability to clean the walls of the root canal and remove the thick layer of the resulting deposits. Layer formed of sediments is a denatured finely divided debris thickness of 1 mm formed on instrumental processed surfaces of the cavity, and is composed of dentin, odontodactylus products, nonspecific inorganic impurities and microorganisms. Removing the layer formed deposits with treated walls of the root canal is the tsya controversial. Its removal provides the best adhesion materials-fillers to dentin and prevents the passage of microorganisms in the tissues of the mouth. Infiltration of microorganisms in the tissues of the mouth should be avoided because they often cause complications leading to treatment failure.

Thus, although the improvement in alternative treatment options of patients with periodontal diseases and other disorders of the mouth and dental disorders will occur in the coming decades, researchers are constantly attempting to improve methods of treatment, because some of them are junk. Accordingly, it would be a real improvement in this area to enhance or even replace the currently used methods of treatment to other to get the best results in the treatment of disorders of the mouth and dental diseases and disorders. Such methods of treatment and compositions disclosed and described in the present invention.

Summary of the invention

The present invention relates to compositions for the care of the oral cavity and, more specifically, to compositions for oral care mouth that contribute to the health of the teeth and chewing gum, including plant components of the Indian mulberry, known the tion in science as Morinda citrifolia L.Morinda citrifoliahas a favorable antimicrobial (antibacterial) properties, while being biocompatible, and thus represents a significant medical potential as part of dental treatment.

In some embodiments, implementation of the present invention are plant components of the Indian mulberry, known in science asMorinda citrifolia L., to treat one or more disorders of the oral cavity or dental disorders, including periodontal diseases such as gingivitis and periodontitis, tooth decay, halitosis, and other irritations of the mouth. The present invention includes plant components of the Indian mulberry as components of compositions for oral care mouth for the treatment and prevention of various disorders of the oral cavity and related to dental disorders. Some embodiments of the present invention includes a component basis excipient and active component that includesMorinda citrifoliaup to fifty percent by weight.

With regard to the foregoing, some embodiments of the present invention relate to a dental preparation for the oral cavity that can cause re immune response in diseases of the gums or razrusheny the teeth.

In addition, some embodiments of the present invention relate to a dental preparation for the oral cavity and method of reducing bleeding gums by reducing inflammation in the gingival sulcus and increase the density of collagen in the gums.

Some embodiments of the present invention relate to a dental preparation for the oral cavity and the method of reducing loss of periodontal ligament by reducing plaque formation and/or adhesion of dental plaque on the teeth and surrounding tissues of the mouth.

Some embodiments of the present invention relate to a dental preparation for the oral cavity and method of reducing the permeability of the walls of the cells of the epithelium to bacterial toxins and, in addition, to reduce the activity collagenase enzymes in the presence of bacteria.

Another objective of certain embodiments of the present invention is to provide a dental product for the oral cavity and method of increasing cellular respiration and oxygen saturation of the cells in the mouth.

Some embodiments of the present invention relate to a dental preparation for the mouth and the way to reduce the frequency and duration of proceeding without suppuration ulcerations of the mouth is alasti.

Some embodiments of the present invention relate to a dental preparation for the oral cavity and method of cleaning teeth, removing spotted painted dirt from the teeth and teeth whitening.

Some embodiments of the present invention relate to a dental preparation for the oral cavity and method of treatment and prevention of gum disease and tooth decay in mammals and, in addition, improve the General health of the oral cavity in General.

In addition, some embodiments of the present invention relate to a dental preparation for the mouth and the way to reduce bad breath in mammals.

These and other features and advantages of the present invention will be mentioned hereinafter or will be more fully apparent from the following description and the accompanying claims. Distinctive features and advantages may be realized and obtained with the use of equipment and combinations specified in the attached claims. In addition, distinctive features and advantages of the invention can be identified in the practical implementation of the invention or will be apparent from the description as follows :

Detailed description of the invention

The present image is eenie can be carried out in other specific forms, without departing from its essence or essential characteristics. Describes the different ways of implementation should be considered in all respects only as illustrative and not as limiting. Scope of the invention, therefore, is specified in the accompanying claims, and not in the following description. All the changes that coincide with the value and fall within the range of equivalents of the claims are covered by the scope of the invention.

As used in the present description, the term "breach of oral or dental violation" refers to any type of disease, condition, properties or impairment that affects the teeth, gums and surrounding tissue of the oral cavity. Examples of such disorders of the oral cavity or dental disorders include periodontal diseases such as gingivitis and periodontitis, tooth decay, bad breath, irritation and damage to the mouth and other conditions affecting the teeth, gums and/or surrounding tissue of the oral cavity. The term "Morinda citrifolia" refers to any component of the plantMorinda citrifolia(L.), including fruit juiceMorinda citrifolia, its extracts, concentrates, fruit juice, its oil, leaves, leaf powder, extracts of leaves, bark, extracts of the bark, roots, extract roots, bark, roots and extracts of the bark of the roots. The term juice Tahitian Noni®" tositsa to the product, which includes recycled components plantsMorinda citrifolia L. In one embodiment, the Tahitian Noni® includes restored fruit juiceMorinda citrifolia L.pure puree juice of French Polynesia. Tahitian Noni® it may also include other natural juices, such as concentrate, natural grapefruit juice concentrate, natural blueberry juice and/or concentrate other natural juice. In the following embodiment, the Tahitian Noni® derived from dried or powderedMorinda citrifolia L. Tahitian Noni® can be obtained from the Morinda Inc., the head office of which is located at 5152 N Edgewood Dr.#100, Provo, UT, 84604.

In the present description, the term "effective amount" refers to the amount sufficient to favorable impacts or achieve the desired results. An effective amount can be entered by one or more injections of applications or dosages. For example, an effective amountMorinda citrifoliais a quantity sufficient to reduce plaque on teeth, inhibit the growth of bacteria and reduce the adhesion of dental plaque, thereby inhibiting the formation of dental caries. Such effective amounts can be determined without additional experiments specialists in this field.

In accordance with this is their invention, components of the plantMorinda citrifolia(L.) can be used in combination with the basis of excipient for the treatment and/or prevention of disorders of the oral cavity or dental disorders. Some embodiments of the present invention may be a food additive, applied topically dental medication or any other form known to the specialists.

The following description of the present invention is grouped into three subheadings, namely "General discussionMorinda citrifoliaand the methods used to obtain processed foodsMorinda citrifolia", "Drugs and methods of administration and Care of the oral cavity". The application of subsections is intended only for the convenience of the reader, and should not be construed as limiting in any sense.

It is obvious that the elements of the present invention, described in General terms and illustrated specific items can be combined and used for a wide variety of different drugs and methods. Thus, the following more detailed description of embodiments of the system and method of the present invention is not intended to limit the scope of the invention as it is set forth in the claims, and is only an illustration of the preferred at the moment the choices done by the means of the present invention.

1. A General discussionMorinda citrifoliaand the methods used to obtain processed foodsMorinda citrifolia

Edisca mulberry or Noni, is known in science asMorinda citrifolia L. (the"Morinda citrifolia"), is a shrub or small tree up to 10 m in height. Leaves elliptical or oval shape, are located opposite each other. Small white flowers are collected in juicy, spherical, resembling the head inflorescence. Fruit large, juicy, egg-shaped. Being Mature, they have a creamy-white color and are edible, but have an unpleasant taste and smell. The plant is native of Southeast Asia and was spread in the early time on the large territory from India to Eastern Polynesia. It grows randomly in the wild, and it was cultivated on plantations and in small individual farms. Flowers ofMorinda citrifolia- small, white, with three to five petals, tubular, fragrant, and about the length of 1.25 see the Flowers develop into fruit, consisting of many small bones, in the United ovoid, ellipsoidal or round knobby fruit with waxy white or greenish-white, or yellowish, translucent skin. The fruit has "eyes" on its surface like potatoes. The fruit is juicy, bitter, light as the tym or yellowish-white and contains numerous red-brown, solid, oblong-triangular, borne on 2-cell granules, each of which contains four seed.

When fully ripe, the fruit has a distinct smell, reminiscent of rancid cheese. Although the fruit is eaten by several nationalities, the most commonly accepted use of plants as a source of red and yellow dyes. Recently, interest in food benefits and favorable action on the health of the plantMorinda citrifoliaadditionally discussed next.

Because the fruit ofMorinda citrifoliais inedible for all practical purposes, the fruit must be processed to make it pleasant for human consumption and included in the food additives used to facilitate the care of the oral cavity. Processed fruit juiceMorinda citrifoliacan be obtained by separating the seeds and peel from the juice and pulp of ripe fruitsMorinda citrifolia; filtering the pulp from the juice and juice packaging. Alternatively, instead of packing juice, it can be immediately included as an ingredient in another food product, frozen or pasteurized. In some embodiments, the implementation of the juice and pulp can be converted into a homogeneous mixture for mixing with other ingredients. Another method involves freeze drying the fruit juice. Fruit and juice can be reset is determined during the final juice product. Other methods include air drying fruits and juices before chopping.

The present invention also encompasses the use of fruit juice and/or puree fruit juice extracted from plantsMorinda citrifolia. In the currently favored method of obtaining fruit juiceMorinda citrifoliathe fruits are harvested either manually or using mechanical equipment. The fruit can be picked when they reach at least one inch (2-3 cm) and up to 12 inches (24-36 cm) in diameter. Fruit preferably have a color varying from dark green through yellow-green until white and having different color gradations within this range. The fruit is thoroughly washed after collection and prior to any processing.

The fruit is left to ripen for a period of 0 to 14 days, with most fruit stand 2 to 3 days. Fruits give you the opportunity to Mature or ripen, placing them on the equipment so that they do not come in contact with the ground. Preferably served with a cloth or mesh material during aging, but they can Mature and open. Being ready for further processing, the fruits are bright in color from light green, light yellow, white or translucent colors. The fruit is inspected for safety or intense green color and firm the th. Depraved and hard green fruit is separated from the usable fruit.

Ripe and vyriausias fruit is preferably placed in covered plastic containers for further processing and transport. Containers with ripe fruit can be stored from 0 to 120 days. Most containers with fruit stand from 7 to 14 days before processing. Containers are optional and can be kept in the refrigerator or at ambient temperature/room temperature before further processing. The fruit extract of containers for storage and processed using manual or mechanical separator. The seeds and skin are separated from the juice and pulp.

The juice and pulp can be Packed into containers for storage and transportation. Alternatively, the juice and pulp can be immediately processed into the final product of the juice. The containers can be stored in the refrigerator, frozen or room temperature conditions.

The juice and pulpMorinda citrifoliapreferably milled into a homogeneous mixture, which can then be mixed with other ingredients such as flavorings, sweeteners, nutritional ingredients, herbal supplements and dyes. The final product of the juice is preferably heated and pasteurized at a minimum temperature of 181°F (83°C) or at a higher temp is the temperature to 212 ° F (100°C).

Other manufactured product is a puree and juice pureeMorinda citrifoliaor in concentrated or diluted form. Puree is essentially the pulp is separated from the seeds, and differs from the described product fruit juice.

Each product downloads and hermetically sealed in the final packaging of plastic, glass or other suitable material that can withstand the processing temperatures. Packaging is maintained at a temperature filling or they can be quickly cooled and then placed in the container for transportation. The container preferably is wrapped in the material in such a way as to maintain or control the temperature of the product in final packaging.

The juice and pulp can be further processed by separating the pulp from the juice using equipment for filtering. Filtering equipment preferably consists of, but is not limited to the above, the centrifuge Cup, mesh filter with a cell size of from 0.01 to 2000 μm, and any other standard commercial filtration devices. The applied pressure during the filtration process usually ranges from 0.1 to about 1000 ft/psi. The flow rate is preferably from 0.1 to 1000 g/min and more preferably from 5 to 50 g/min Wet pulp is washed and filtered at least once and up to 10 times to completely remove the juice from the pulp. The wet pulp typically has a fiber content of 10 to 40 percent by weight. The wet pulp is preferably pasteurized at a minimum temperature of 181°F (83°C) and then Packed in a drum-type containers for further processing or manufacturing of the high fiber content of the product.

Recycled productMorinda citrifoliaalso can exist in the form of dietary fiber. Also redesigned the productMorinda citrifoliaalso can exist in the form of oil. OilMorinda citrifoliausually includes a mixture of several different fatty acids in the form of triglycerides, such as palmitic, oleic and linoleic fatty acids or other fatty acids present in smaller quantities. In addition, the oil preferably includes an antioxidant to inhibit deterioration of the oil. Preferably use conventional antioxidants nutritional quality.

Drying can be further processed raw flesh. The drying methods may include drying by freezing (freeze drying), drum drying, drying in the sun and spray drying. The dried pulpMorinda citrifoliamay contain moisture in the range from 0.1 to 15 percent by weight and more preferably from 5 to 10 percent by weight. The dried pulp preferably has a fiber content in the range from 0.1 to 30 percent by weight or more is predpochtitelno from 5 to 15 percent by weight.

High product may include wet or dry pulpMorinda citrifoliaadditional fibrous ingredients, water, sweeteners, flavorings, colorants and/or nutritional ingredients. Additional fibrous ingredients may include fiber products plant-based, or commercially available or developed privately. Examples of some typical fibrous products are guar gum, Arabia gum, soy fiber, oat fiber, pea fiber, shell from the pulp of citrus, hydroxymethylcellulose, cellulose, algae, lumber or wood pulp food quality, hemicellulose, etc. Other additional fibrous ingredients can be obtained from grain or grain products. The concentration of these other source of fibers typically range from 0 to 30 percent by weight and more preferably from 10 to 30 percent by weight.

Typical sweeteners include, but are not limited to the above, the natural sugar derived from corn, sugar beet, sugar cane, potato, tapioca or other starchy sources, which can be chemically or enzymatically converted to crystalline chunks, powders, and/or syrups. In addition, sweeteners may consist of artificial is whether high-intensity sweeteners, some of which are aspartame, Sucralose, stevia, saccharin, etc. are the Concentration of sweeteners can be from 0 to 50 percent by weight relative to the total weight of the composition and more preferably from 1 to 5 percent by weight.

Typical flavorings may include, but are not limited to specified, artificial and/or natural flavorings or ingredients that contribute in palatability. The concentration of flavors may vary, for example, from 0 to 15 percent by weight of the total weight of the composition. The dyes may include coloring agents, food quality or natural coloring agents having a concentration ranging from 0 to 10 percent by weight of the formula.

Typical nutritional ingredients may include vitamins, minerals, trace elements, herbs, Botanical extracts, bioactive chemical compounds and compounds in concentrations from 0 to 10 percent by weight. Examples of vitamins that can be added to the composition of the fibers include, but are not limited to specified, vitamins a, B1, B12, C, D, E, folic acid, Pantothenic acid, Biotin, etc. are Examples of minerals and trace elements that can be added to the composition of the fibers include, but are not limited to specified, calcium, chromium, copper, cobalt, boron, magnesium, iron, selenium, manganese, molybdenum, potassium, iodine,zinc, phosphorus etc. Herbs and Botanical ecstacy include, but are not limited to specified, Echinacea root extract, Gingko biloba, horsetail forest, Indian mulberry, shiitake mushrooms, spirulina algae, grape seed extract, etc. Typical of bioactive chemicals may include, but are not limited to specified, caffeine, ephedrine, L-carnitine, creatine, lycopene, etc.

The juice and pulp can be dried using a variety of methods. The mixture of juice and pulp can be pasteurized or enzymatic treated before drying. Enzymatic method begins by heating the product to a temperature between 75 and 135°F. It is then treated or separate enzyme or combination of enzymes. These enzymes include, but are not limited to specified, amylase, lipase, protease, cellulase, bromelain, etc. Juice and pulp can be dried together with other ingredients, such as described above in connection with the high fiber content of the product. Typical nutritional profile of the dried juice and pulp contains from 1 to 20 percent of water, from 0.1 to 15 percent protein, from 0.1 to 20 percent fiber and vitamins and minerals.

The filtered juice and water, obtained by washing the crude pulp, preferably mixed together. The filtered juice can be one stripped off under vacuum to a volume of from 40 to 70 and content of the logs from 0.1 to 80 percent, more preferably from 25 to 75 percent. The concentrated juiceMorinda citrifoliacan be pasteurized or not pasteurized. For example, the juice must be pasteurized in the case, when the sugar content or blagochinnost were low enough to prevent microbial growth. It is Packed for storage, transportation and/or additional processing.

In accordance with the present invention a composition for caring for the oral cavity is in the form of a dietary Supplement or applied topically oral dental drug or other form used for the treatment and/or prevention of one or more disorders of the oral cavity or dental disorders. The number used for the treatment may depend on many factors, including the type of disorder of the oral cavity or dental disorders, the patient's physical characteristics, etc.

The plantMorinda citrifoliarich content of natural ingredients. These ingredients, which have been identified include: (of leaves): alanine, anthraquinones, arginine, ascorbic acid, aspartic acid, calcium, beta-carotene, cysteine, cystine, glycine, glutamic acid, glycosides, histidine, iron, leucine, isoleucine, methionine, Niacin, phenylalanine, phosphorus, Proline, resin, Riboflavin, serine, beta-sitosterol, thiamine,threonine, tryptophan, tyrosine, orsolino acid and valine; (of flowers): acacetin-7-o-beta-d(+)-glucopyranose, 5,7-dimethyl-apigenin-4'-o-beta-d(+)galactopyranoside and 6.8-dimethoxy-3-methylanthracene-1-o-beta-rhamnosyl-glucopyranoside; (of fruit): acetic acid, asperuloside, butane acid, benzoic acid, benzyl alcohol, 1-butanol, Caprylic acid, dekanovu acid, (E)-6 dodecene-gamma-lactone, (Z,Z,Z)-8,11,14-eicosatrienoic acid, Sadovoy acid, etilgexanol, ethylhexanoic, ethyloctanoic, Etisalat, (Z)-6-(ethylthiomethyl)benzene, eugenol, glucose, heptane acid, 2-heptanon, hexanal, hexanamide, hexandiol acid, hexanoic acid (gekaeva acid), 1-hexanol, 3-hydroxy-2-butanone, lauric acid, lemon, linaeve acid, 2-methylbutanoyl acid, 3-methyl-2-butene-1-ol, 3-methyl-3-butene-1-ol, metalmechanic, metalloids, methylhexanoate, methyl 3-methylthiopropionate, methyloctanoic, methyl oleate, metrpolitan, 2-methylpropanoyl acid, 3-methylthiopropionate acid, myristic acid, nonanoyl acid, octanoic acid (Oktava acid), oleic acid, palmitic acid, potassium, scopoletin, undecanoyl acid, (Z,Z)-2,5-undecadien-1-ol and woeful; (from the roots): anthraquinones, asperuloside (rubyhornet acid), damnacanthal, glycosides, Morondava, morindin, morindin, mucilaginous substances, nor is damnacanthal, rubiadin, onomatology simple ether rubiadin, resin, arancibia, steady and trihydroxysilyl Antoine-onomatology simple esters; (root bark): alizarin, horrobin, glycosides (pentose, hexose), Morondava, marinangel, morindin, morindin, retinoid substance, onomatology simple ether rubiadin and arancibia; (of wood): astragalo-2,3-dimethyl simple ester; (from tissue culture): damnacanthal, lucigen, lucigen-3-premieroil and Merendon-beta-pyreverse; (of plants): alizarin, alizarin-alpha methyl simple ether, anthraquinones, asperuloside, hexanoic acid, Morondava, morindin, morindland, octanoic acid and oralbuy acid. The present invention involves the use of all parts of the plantMorinda citrifoliaseparately, in combination with each other or in combination with other ingredients. The above parts of the plantM. citrifolianot an exhaustive list of the used parts of the plant, and are for illustration purposes only. Thus, although some parts of the plantM. citrifolianot noted above (for example, seeds, fruits, pericarpel fruit, tree bark) the present invention involves the use of all parts of the plant.

Recently, as noted, it was discovered many health benefits based on the use of products containingMrinda citrifolia . One of the identified benefitsMorinda citrifolialies in its ability to distinguish and produce xeronine, which is a small alkaloid possessing physiological activity in the body. Xeronine exists in all healthy cells of plants, animals and microorganisms. Even despite the fact thatMorinda citrifoliacontains negligible amount of free of xeronine, it contains appreciable quantities of predecessor xeronine called proxeronine. In addition,Morinda citrifoliacontains an inactive form of the enzyme proxeronine, which releases xeronine of proxeronine. In an article entitled "The Pharmacologically Active Ingredient of Noni" (Pharmacologically active ingredient of Noni) R.M.Heinicke, University of Hawaii, States thatMorinda citrifoliarepresents the "best raw material for application to selection of xeronine" due to the presence of building blocks proxeronine and proxeronine. These building blocks contribute to the selection and production of xeronine in the body. Action essential nutrient of xeronine is fourfold.

First, xeronine activate at rest enzymes identified in the small intestine. These enzymes are crucial for effective digestion, calm the nervous system and General the th physical and emotional energy.

Secondly, xeronine protects and maintains the shape and elasticity of the protein molecules so that they are able to pass through cell walls and used for the formation of healthy tissue. Without additions of these nutrients into the cell, the cell is unable to effectively do their jobs. Our cells and subsequently the body suffer, in the absence of proxeronine for the production of xeronine.

Thirdly, xeronine helps to expand the pores of the membranes of cells. This extension allows large chains of peptides (amino acids or proteins) to pass into the cell. If these circuits are not used, they become waste.

Fourthly, xeronine, which is obtained from proxeronine, increases pores for better absorption of nutrients.

Each tissue has cells that contain proteins, which are receptor sites for absorption of xeronine. Some of these proteins are inactive forms of enzymes that require absorption of xeronine to become active. Thus, xeronine by transformation in procollagenase system of the body in specific protease safely and quickly removes dead tissue from the skin. Other proteins become potential sites of receptors for hormones after interaction with xeronine. Thus the actionMorinda citrifolia,leading to the well-being of the individual, probably caused by xeronine, converting some receptor proteins of the brain in the active sites for the absorption of endorphins, the feel-good hormone. Other proteins form a pore through the membrane in the intestine, blood vessels and other organs of the body. Absorption of xeronine these proteins causes a change in shape of cells and thus affects the passage of molecules through the membrane.

It is known that the many advantages ofMorinda citrifoliahas a number of separate actions in the case of individuals with cancer, arthritis, headaches, digestive disorders, malignant tumors, broken bones, high blood pressure, diabetes, pain, infection, asthma, toothache, physical defects, immune system disorders, and others.

Compositions containingMorinda citrifoliamay be in a form suitable for oral administration, for example in the form of tablets or lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsions, syrups or elixirs. Compositions intended for oral use can be obtained in accordance with any method known in this field for the manufacture of compositionsMorinda citrifoliaand such compositions may contain one or more agents selected from the group consisting of sweeteners, aromatization is, dyes and preservatives. Tablets containMorinda citrifoliain a mixture with non-toxic pharmaceutically acceptable excipients which are suitable for receiving tablets. These excipients, for example, can be an inert diluents, granulating and dezintegriruetsja agents, binding agents and lubricants. Tablets can be without shell or they can be coated using known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a prolonged action over a longer period of time. For example, you can use slow down time a substance such as glycerol monostearate or distearate glycerin.

Aqueous suspensions contain theMorinda citrifoliain a mixture with excipients suitable for receiving water suspensions. Such excipients are suspendresume agents, such as carboxymethylcellulose sodium, methylcellulose, hypromellose, sodium alginate, polyvinylpyrrolidone, tragacanth gum and Arabic gum; dispersing or wetting agents may be a natural phosphated, for example lecithin, or condensation products of accelerated with fatty acids, for example polyoxyethylene, or condensation products is telenokia with long chain aliphatic alcohols, for example heptadecafluorooctane, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol, such as polyoxyethylenesorbitan, or condensation products of ethylene oxide with partial esters derived from fatty acids and anhydrides hexitol, such as polyoxyethylenesorbitan.

2. Drugs and routes of administration

The present invention relates to methods of caring for the oral cavity and drugs, includingMorinda citrifoliasuch as toothpaste, gels, tooth powders, liquids for rinsing the mouth, gargle for mouth, gum, sprays and mouth lozenges containingMorinda citrifolia.Morinda citrifoliacan be represented in the form of fruit juices, extracts, concentrates, fruit juice, oil, powder of leaves or leaf extract.Morinda citrifoliainjected in various media or food compositions suitable for the treatment of mammals in vivo. RevisedMorinda citrifoliamay be included in a toothpaste or gel, powder, granules, dezintegriruetsja tablets, rinse mouth, lozenges or chewing gum. The composition may further include water, flavoring agents, active substances, emulsifiers, alcohol, sweeteners, thickeners, surfactants, knit what it means and tonic medicinal extracts, proofreaders taste, abrasives and polishing agents, deodorizing agents, preservatives, flavoring buffers, whitening agents, wound healing and inhibiting inflammatory substances, colorants, dyes, pigments, abrasives, polishing agents, antimicrobial agents, pH buffers, and other adjuvants and fillers. Oral compositions for the care of the oral cavity can also include water, flavors, and other active compounds. Advanced composition for care of the oral cavity can include other ingredients selected from the group consisting of an emulsifier, alcohol, sweeteners, thickeners, surfactants, binders and tonic medicinal extracts, proofreaders taste, abrasive or polishing substances, deodorizing agents, preservatives, flavoring buffers, whitening agents, wound healing and inhibiting inflammation substances, coloring agents, dyes, pigments, abrasives, polishing substances, antimicrobial agents, pH buffers, and the like and combinations thereof, as well as other auxiliary additives and fillers, the choice and the number of which will depend on the nature of the composition for oral care mouth.

The active ingredients can be extracted from various parts of the plantMorinda citrifoliaand the use of various alcohols, or solutions based on alcohols, such as methanol, ethanol and ethyl acetate, and other derivatives on the basis of alcohols using any known method in this field. Recycled productMorinda citrifoliais an active ingredient or contains one or more active ingredients, such as quercetin, rutin, and others, to care of the oral cavity. Active ingredients quercetin and rutin present in amounts by weight, fluctuating in the range from 0.01 to 10 percent by weight of the total preparation or composition. These quantities can be concentrated to a higher concentration in which they are present in quantities of from 10 to 100 percent. Additionally, in the present invention refers to chemical and mechanical methods of extraction, including chromatographic system.

Recycled productMorinda citrifoliacan be entered in the formulations with various other ingredients to produce different compositions, such as food compositions, compositions for internal use or other. Ingredients for use in food compositions can be any that are safe for ingestion by a mammal, in particular humans, and composition can exist in various forms, such as liquids, tablets, lozenges, aqueous or oil Rast is ora, dispersible powders or granules, emulsions, syrups, elixirs, etc. moreover, because the food composition is most likely to be used orally, it can contain one or more agents selected from the group consisting of sweeteners, flavors, colorants, preservatives and other medicinal agents, as specified.

The carrier medium may include any ingredient that can be introduced into the body of the mammal and which is capable of providing a carrier medium for recycled productMorinda citrifolia. Specific drugs environments carriers well known in the field and are not described in detail in the present description. The purpose of the carrier medium is, as indicated, to provide the means for delivery of recycled productMorinda citrifoliain the body of the patient. The media components of the present compositions can be any orally acceptable carrier, suitable for use in the oral cavity. Such media include conventional components of toothpastes, tooth powders, prophylactic pastes, pastilles, chewing gum and the like and will be described in detail next.

Flavors and sweeteners

The flavors used in the invention can be any flavor or pharmaceuticas and acceptable flavor and specific flavors will depend on the type of composition to care for the oral cavity. Preferably, the flavoring includes natural aromatic oils, including selected from the group consisting of oil of peppermint, oil of gaultheria supine, oil curly mint, clove oil from flower buds, Petrushenko oil, eucalyptus oil and the like. You can also use a combination of oils and oils with other flavors. Suitable flavoring agents may be selected from a list that includes menthol, mental, anethole, methyl salicylate, eucalyptol, Chinese cinnamon oil, 1-methyl acetate, sage, eugenol, Oksanen, alpha ireson, marjoram, lemon, orange, propenolatomethyl, cinnamon, vanilla, thymol, linalool, glycerinate cinnamic aldehyde, and the like, and combinations thereof. The flavoring may include combinations of natural aromatic oils and other flavorings, such as the above compounds. The flavoring may include cooling agents such as menthol, N-substituted p-Menten-3-carboxamide (such as N-ethyl-p-methane-3-carboxamide), 3,1-methoxypropane-1,2-diol and the like, and combinations thereof. Flavoring agents are generally used in the compositions at levels from about 0.001 to about 5% by weight of the composition.

In the liquid compositions for oral cavity, rinse for Polo the tees mouth, spray to the oral cavity, chewing gum or lozenges can use any sweetener food quality and/or pharmaceutically acceptable sweeteners, including saccharin, fructose, xylitol, salt saccharin, thaumatin, aspartame, D-tryptophan, dihydrochalcones, Acesulfame and cyclamate salt. In particular the sodium cyclamate and saccharin sodium, and combinations thereof.

In addition to flavorings and sweetener as an optional ingredient in the compositions of the present invention can be used cooling agents that cause salivation agents, warming agents, and cause the effect of numbing agents. Preferred warming agents include capsicum and nicotinate esters, such as benzylsuccinic. Preferred causing effects numbness agents include benzocaine, lidocaine, clove oil from flower buds and ethanol. These agents are present in the compositions at levels from about 0.001 to about 10%, preferably from about 0.1 to about 1% by weight of the composition.

The cooling agent can represent one of a variety of substances. These include such substances as carboxamide, menthol, ketals, diols, and mixtures thereof. Preferred cooling agents in the present compositions are parameterbased agents, such as N-ethyl-p-methane-3-carboxamid is, known as "WS-3", N-trimethyl-2-isopropylmalonic, known as "WS-23", and their mixtures. Additional preferred cooling agents are selected from the group consisting of menthol, 3-1-methoxypropan-1.2-diol acetal of pentaglycine and mantelatto. Terms menthol and Menthyl as used in this description, include the right - and levogyrate isomers of these compounds and their racemic mixture. TC-10 is described in U.S. patent No. 4459425, Amano et al., issued July 10, 1984 WS-3 and other agents described in U.S. patent No. 4136163, Watson et al., issued on January 23, 1979

Other active ingredients

Active compounds of the composition to care for your mouth will depend on the nature and application of the composition. Usually active compounds for oral care mouth mask the unpleasant smell from the mouth, destroying chemical compounds that lead to the unpleasant smell from the mouth, kill or inhibit the growth of bacteria in the mouth that cause odor or halitosis, destroy wine stone, remove dirt from the teeth and mouth, and/or whiten the teeth. For example, when the composition for the care of the oral cavity in the form of liquids for rinsing the oral cavity, means for rinsing the oral cavity, chewing gum, sprays, oral, pastilles and the like, the active components include hygienically the active substance to the oral cavity, antibacterial substances that reduce the sensitivity of agents, agents against plaque formation, and combinations thereof, such as selected from the group consisting of chlorine dioxide, fluoride, alcohols, triclosan, domainbased, chloride of cetylpyridinium, calcium lactate, salts of calcium lactate and the like and combinations thereof. In embodiments of the invention, when the composition for the care of the oral cavity represent a tooth powder or tooth paste, such as toothpaste, gels and the like, the active components include hygienic active substances to the mouth, antibacterial agents, reducing sensitivity agents, agents against plaque formation, and combinations thereof, such as selected from the group consisting of sodium fluoride, fluoride of divalent tin, monophosphate sodium, triclosan, chloride of cetylpyridinium, zinc salts, pyrophosphate, calcium lactate, salts of calcium lactate 1-hydroxyethane-1,2-diphosphonic acid, 1-phosphonopropyl-1,2,3-tricarboxylic acid, azacycloheptane-2,2-diphosphonic acids, cyclic aminophosphonic acids and the like and combinations thereof.

The present compositions to the oral cavity may also include other active agents, such as antimicrobial agents. These include such agents as water-insoluble nicotianae antimicrobial Agay is you, such as halogenated diphenyl ethers, phenolic compounds including phenol and its homologs, mono - and polyalkylene and aromatic halogenfrei, resorcinol and its derivatives, bisphenol compounds and halogenated salicylanilide, esters of benzoic acid and halogenated carbanilide. Water-soluble antimicrobial agents include Quaternary ammonium salts and bis-biguanidine salt along with other things. Triclosan monophosphate is an additional water-soluble antimicrobial agent. Quaternary ammonium agents include those in which one or two of the substituents on the Quaternary nitrogen atom have a carbon chain length (typically alkyl group) from about 8 to about 20, typically from about 10 to about 18 carbon atoms while the remaining substitutes (typically alkyl or benzyl group) have a lower number of carbon atoms, such as from about 1 to about 7 carbon atoms, typically a methyl or ethyl group. Bromide of dodecyltrimethylammonium, chloride tetradecylbenzene, bromide, domiphen, chloride, N-tetradecyl-4-ethyl pyridinium, bromide, dodecyldimethyl(2-phenoxyethyl)ammonium, chloride of benzyldimethylamine, chloride cetylpyridinium, quaternionic 5-amino-1,3-bis(2-ethylhexyl)-5-methyl-hexahydropyridine, chloride benzalconia,chloride benzene and chloride methylbenzene are examples of conventional Quaternary ammonium antibacterial agents. Other compounds are bis[4-(R-amino)-1-pyridine]alkanes, as described in U.S. patent No. 4206215 issued Bailey 3 June 1980, Other antimicrobial agents, such as bisglycinate copper, glycinate copper, zinc citrate and zinc lactate, can also be included in the compositions. Enzymes and other types of active substances can be used in these compositions. Useful enzymes include those that belong to the category of proteases that cleave enzymes, inhibitors of matrix of dental plaque and oxidase. Proteases include papain, pepsin, trypsin, fitsin, bromelain; splitting the cell wall enzymes include secrete lysozyme; inhibitors of matrix of dental plaque include dextranase, atanazy; and oxidases include glucose oxidase, lactate oxidase, galactose oxidase, oxidase uric acid, peroxidase, including horseradish peroxidase, myeloperoxidase, lactoperoxidase, chloroperoxidase. Oxidase also have whitening/cleaning activity in addition to antimicrobial properties. Such agents are described in U.S. patent No. 2946725 issued by Norris and others, July 26, 1960, and U.S. patent No. 4051234 issued by Gieske et al., September 27, 1977, Other antimicrobial agents include chlorhexidine, triclosan, triclosan monophosphate and aromatic oils such as thymol. Triclosan and other agents of this type are described Parran., r., and al. in U.S. patent No. 5015466, issued may 14, 1991, and U.S. patent No. 4894220, issued January 16, 1990, Nabi These and other agents that benefit against plaque may be present at levels from about 0.01 to about 5.0 percent by weight of the composition of the tooth powder or tooth paste.

Thickeners

Upon receipt of toothpaste or gels, you can add a little thickening material to provide the desired consistency of the composition to provide the desired release characteristics of the active substances in the application, to ensure stability during storage and to ensure the stability of the composition, etc. Preferred thickeners are carboxyvinyl polymers, carrageen, hydroxyethyl cellulose, laponite and simple soluble salts of cellulose ethers such as carboxymethylcellulose sodium and karboksimetiltselljuloza sodium. You can also use natural gums, such as Karay gum, xanthan gum, Arabic gum and tragacanth gum. Colloidal marialullaby silicate or finely dispersed silicon dioxide can be used as part of the thickening agent to further improve the texture.

The thickener or binder for tooth powder or tooth paste can be selected from groups who, consisting of fine particles of silica gels and nonionic hydrocolloids, such as carboxymethylcellulose, hydroxymethylcellulose sodium, hydroxyethylcellulose, hydroxypropionate guar gum, hydroxyethyloxy starch, polyvinylpyrrolidone, vegetable gums, such as tragacanth gum, agar, carrageen, Arabian gum, xanthan gum, guar gum, gum seeds of acacia, carboxyvinyl polymers, evaporated silicon dioxide, silicon clay and the like and combinations thereof. The thickener or binder can be used in conjunction with the carrier or without media, such as glycerin, polyethylene glycol (PEG-400) or their combination, however, when using the carrier, up to about 5% thickening agent or binder, preferably from about 0.1 to about 1.0%, combined with from about 95,0 to about 99.9% of the carrier, preferably from about 99,0 to about 99.9 percent, based on the total weight of the combination of the thickener/media. A preferred class of thickeners or gelling agents includes the class of homopolymers of acrylic acid crosslinked with alkylamine ethers of pentaerythritol or alkylamine ethers of sucrose or Carbonari.

Copolymers of lactide monomers and glycolide, the copolymer having a molecular weight in the range from about 1000 is about 120000 (srednecenovogo), useful for delivery of active agents in the periodontal pocket or around the periodontal pockets as submessage gel media". These polymers are described in U.S. patent No. 5198220, issued March 30, 1993, and U.S. patent No. 5242910, issued September 7, 1993, both issued Damani, and U.S. patent No. 4443430 issued Mattei 17 April 1984 Thickeners can be used in an amount of from about 0.1 to about 15%, preferably from about 2 to about 10%, more preferably from about 4 to about 8% by weight of the total weight of the composition toothpaste or gel. Higher concentrations can be used for chewing gum, lozenges (including mint flavored breath fresheners), Sasha, non-abrasive gels and subdecay gels.

In the compositions of the fluids of the mouth, rinse the mouth, spray for the mouth, chewing gum or lozenges can use any thickener or filler food grade or pharmaceutically acceptable. The thickener or filler can be dispersed in a medium such as glycerine, polyethylene glycol or their combinations (the dispersion of the thickener/media). Thickeners and fillers are those selected from the group consisting of xanthan gum, polymeric complex polyester compounds, natural gums (e.g., Karay gum, Arabian Kama is ü, tragacanth gum), Cartagena, hydroxyethylcellulose, methyl cellulose, carboxymethyl cellulose, powder flour from underground shoots or rhizomes arrowroot starch, in particular corn starch and potato starch and the like, and combinations thereof. The thickener or filler can be used in conjunction with the carrier or without the media, however, when using the carrier, up to about 5% of a thickener or filler, preferably from about 0.1 to about 1.0%, combined with from about 95,0 to about 99.9% of the carrier, preferably from about 99,0 to about 99.9 percent, based on the total weight of the combination of the thickener/media.

Giving opacity agents

Giving opacity agents that can be used in the compositions of the fluids of the mouth, rinse the mouth, spray to the oral cavity, chewing gum or lozenges, include selected from the group consisting of calcium citrate, esters of rosin, of an emulsion of vegetable gums, triglycerides of Caprylic/capric acids, some gums, such as guar gum or Arabian gum, and high-stability oils.

Abrasives or polishing agents

You can use any standard abrasives or polishing agents, including selected from the group consisting of chalk, calcium carbonate is Oia, of dicalcium phosphate, insoluble metaphosphate sodium, aluminum silicate, calcium pyrophosphate, finely dispersed synthetic resins in the form of particles, silicates, aluminum oxide, three-hydrate of alumina, hydroxyapatite and the like or combinations thereof. Abrasives or polishing agents preferably may consist wholly or mainly fine xerogel of silica, the hydrogel silicon dioxide, precipitated silicon dioxide, alumina trihydrate and fine aluminum oxide in the form of particles, and combinations thereof.

Surfactants

Surfactants that can be used in toothpastes or gels, are selected from the group consisting of anionic viscoplastic surfactants, such as linear C12-18the sodium alkyl sulphates; sodium salt With12-16linear alilovic polyglycolic of ethersulfate containing from 2 to 6 glycol ether groups in the molecule; alkyl-(C12-16)benzosulfimide; linear alkane-(C12-18)-sulfonates; monoalkyl-(C12-18)-ethers sulfonterol acids; sulfated monoglycerides of fatty acids; sulfated of alkanolamide fatty acids; alkyl-(C12-18)-ethers sulfoxyde acid and acylcarnitine, acetamido and ACI is isocyanato, all of which contain from 8 to 18 carbon atoms in the acyl fragment. Non-ionic surfactants such as ethoxylates, mono - and diglycerides of fatty acids, esters of sorbitol and fatty acids, and copolymers of ethylene oxide-propylene oxide are also suitable. Particularly preferred surfactants are sodium lauryl sulphate and sarcosinate. You can use a combination of surface-active substances.

One of the preferred optional agents of the present invention is a surfactant, preferably selected from the group consisting of sarcosinate surfactants, isethionate surfactants and taurate surfactants. Preferred for use in this invention are alkali metal salts or ammonium salts of data surfactants. Most preferred are the sodium and potassium salts of the following substances: laurylsarcosine, myristoleate, palmitoylcarnitine, stearoylethanolamine and oleoresins. This surfactant may be present in the compositions of the present invention in an amount of from about 0.1 to about 2.5%, preferably from about 0.3 to about 2.5% and most predpochtitelno about 0.5 to about 2.0 percent by weight of the total composition. In the compositions of the present invention is not necessarily possible to use other suitable compatible surfactant or in combination with sarcosinates surface-active substance. Suitable optional surfactants are described in more detail in U.S. patent No. 3959458, issued may 25, 1976, Agricola and others; US patent No. 3937807, issued February 10, 1976 Haefele; and U.S. patent No. 4051234, issued on 27 September 1988 Gieske and other

Preferred anionic surfactants used in the present invention include water-soluble salts of alkyl sulphates having from 10 to 18 carbon atoms in the alkyl radical, and soluble salts from sulphonated of monoglycerides of fatty acids having from 10 to 18 carbon atoms. Sodium lauryl sulfate and coconut monoglycerides sodium are examples of anionic surfactants of this type. It is also possible to use mixtures of anionic surface-active substances.

Preferred cationic surfactants that can be used in the present invention can be broadly defined as derivatives of aliphatic Quaternary ammonium compounds having one long alkyl chain containing from 8 to 18 carbon atoms, such as chloride of lauryldimethylamine, chloride cetiner the Shandong Jinlong, bromide of cetyltrimethylammonium, chloride di-isobutylphthalate-dimethylbenzylamine; nitrite coconut alkyltrimethylammonium, fluoride of cetylpyridinium etc. Preferred compounds are the Quaternary ammonium fluorides described in U.S. patent No. 3535421, issued October 20, 1970 Briner, etc. where these fluorides of Quaternary ammonium possess detergent properties. Some cationic surfactants can also act as bactericides in the compositions described herein. Cationic surfactants, such as chlorhexidine, though, and are suitable for use in the present invention, are not preferred due to their ability to paint hard tissues of the oral cavity. Specialists in this area is known for the opportunity, and cationic surfactants should be included in the compositions, only taking into account this constraint.

Preferred nonionic surfactants that can be used in the present invention can be broadly defined as compounds produced by the condensation of groups alkalisation (hydrophilic in nature) with an organic hydrophobic compound which may be aliphatic or alkylaromatic in nature. Examples of suitable nonionic surface is STN-active substances include pluronic, condensates of polyethylene oxide and alkyl phenols, products derived from the condensation of ethylene oxide with the product of the interaction of propylene oxide and Ethylenediamine, condensates of ethylene oxide with aliphatic alcohols, oxides, long chain tertiary amines, oxides, long chain tertiary phosphine, a long-chain diallylsulfide and mixtures of these substances.

Preferred zwitterionic synthetic surfactants that can be used in the present invention can be broadly described as derivatives of aliphatic Quaternary ammonium, fofanah and Solonevich compounds in which the aliphatic radicals can be linear or branched and where one of the aliphatic substituents contains from about 8 to 18 carbon atoms, and the other contains anionic contributing to solubilize in the water group, such as carboxyl, sulphonate, sulphate, phosphate or phosphonate.

Preferred betainovuyu surfactants described in U.S. patent No. 5180577 Polefka and others, issued January 19, 1993, a Typical alkyldiphenylamine include decile betaine or 2-(N-decyl-N,N-dimethylammonio)acetate, Comblain or 2-(N-Kok-N,N-dimethylammonio)acetate, myristylated, palmitoylation, laurylether, zeilboten, stearylamine and so on Aminobutane polluter is designed by cocoamidopropybetaine, cocamidopropylbetaine, lauramidopropyl and the like. Selected betaines preferably represent cocamidopropylbetaine and more preferably lauramidopropyl.

Chelating agents

Another preferred binding agent is a chelating agent such as tartaric acid and its pharmaceutically acceptable salts, citric acid and alkali metal citrates and mixtures thereof. Chelating agents capable of forming a complex with the calcium found in the cell walls of bacteria. Chelating agents can also destroy plaque by removing calcium from the calcium bridges that help the biomass maintained in nastraivaemim condition. However, it is undesirable to use a chelating agent, the affinity of the calcium is too high, as this can lead to demineralization of the teeth, which is contrary to the objectives and intent of the present invention.

Sodium citrate and potassium are the preferred alkali metal citrates, while the citrate is the most preferred. Also preferred is a combination of citric acid/citrate of an alkali metal. Preferred in the present invention are alkali metal salts of tartaric acid. Most preferred for use in this and the finding are disodium tartrate, dicale tartrate, sodium potassium tartrate, hydrotartrate sodium and hydrotartrate potassium. The amount of chelating agent suitable for use in the present invention ranges from about 0.1 to about 2.5%, preferably from about 0.5 to about 2.5% and more preferably from about 1.0 to about 2.5%. Chelating agent, which represents a salt of tartaric acid, can be used separately or in combination with other optional chelating agents.

You can use other optional chelating agents. Preferably such chelating agents have a binding constant of the calcium from about 101up to 105to ensure superior cleaning properties with reduced formation of plaque and calculus.

Another group of agents that are suitable for use as chelating agents in the present invention, form a soluble pyrophosphates. Salts of pyrophosphates used in these compositions can be any of pyrophosphate salts of an alkali metal. Specific salts include Tetra - (alkali metal)pyrophosphate, di(alkali metal) likely pyrophosphate, three(alkali metal) monocyclic pyrophosphate and mixtures thereof, where alkali metals are preferably represents sodium or potassium. Salt used in their hydrated, t is to UN-hydrated forms. An effective amount pyrophosphate salt used in the present composition, is usually sufficient to provide at least 1.0% of pyrophosphatase ion, preferably from about 1.5 to about 6%, more preferably from about 3.5 to about 6% of such ions. It should be understood that the level pyrophosphate ions is such that is able to provide the composition (i.e., theoretical quantity at a suitable pH), and that pyrophosphate form, different from P2About7may be present when the pH of the final product. Pyrophosphate salts are described in more detail in Kirk &Othmer, Encyclopedia of Chemical technology, Second Edition, Volume 15, Interscience Publishers (1968).

Optional agents, which are used instead of, or in combination with the pyrophosphate salt include such well-known substances as polyaminopropyl acid (AMPS), the trihydrate of zinc citrate, polyphosphates (e.g., tripolyphosphate, hexametaphosphate), diphosphonates (e.g., EHDP, AHP), polyphosphate, phosphonate copolymers, polypeptides (such as poliasparaginovaya and polyglutamine acid) and mixtures thereof. Examples of phosphonate copolymers are polymers based diphosphonate described in U.S. patent No. 5011913 issued by Benedict and other Preferred polymer is modified by diphosphonates polyacrylic acid. Appropriate postnationalism polymers are described in U.S. patent No. 5980776, issued by Zakikhani et al.

Polyphosphates are also optionally included in the present compositions. The term polyphosphate usually realize that the substance contains two or more phosphate molecules arranged in a linear configuration, although there may be some cyclic derivatives. In addition to the pyrophosphates and tripolyphosphate, which represent technical polyphosphates, also along with other desirable polyphosphates having four or more phosphate fragments, i.e. metropolitical and sodium hexametaphosphate. Polyphosphates larger than Metropolitanate, usually exist in the form of an amorphous glassy material. Preferred in the present invention are linear "glassy" polyphosphates. These polyphosphates may be used individually or in combination.

Another possible group of chelating agents suitable for use in the present invention are polymeric polycarboxylate. Such materials are well known in this field and are used in the form of their free acids or partially or preferably fully neutralized water soluble alkali metal salts (e.g. potassium and preferably sodium) or ammonium salts. Preferred are copolymers in a ratio of from 1:4 to 4:1 of maleic anhydride or acid with another which they are polymerized ethyleneamines monomer, having a molecular weight (MW) from about 30,000 to about 1000000.

Other functional polymer polycarboxylate include, for example, the copolymers in the ratio of 1:1 maleic anhydride with acrylate, hydroxyethylmethacrylate, N-vinyl-2-pyrrolidone or ethylene and copolymers of acrylic acid with methyl or hydroxyethylmethacrylate, methyl - or acrylate, isobutylparaben ether or N-vinyl-2-pyrrolidone.

Additional functional polymer polycarboxylate described in U.S. patent No. 4138477 issued Gaffar February 6, 1979, and U.S. patent No. 4182914 issued Gaffar January 15, 1980, and include copolymers of maleic anhydride with styrene, isobutylene or ethylvanillin ether, polyacrylic, politekonomii and primulinum acids and sulfoacetate the oligomers having a molecular weight of such low as 1000, and is available as Uniroyal ND-2.

The fluorine source

Generally accepted to contain additional water-soluble compound of fluorine present in the means for brushing your teeth and other compositions for the oral cavity in a quantity sufficient to create a concentration of fluoride ions in the composition at 25°and/or when using it from about 0,0025 to about 5.0 percent by weight, preferably from roughly 0.005 to about 2.0 percent by weight, to provide additional protiveris the activity. As sources of soluble fluoride in the present compositions can be used a great many giving ion of fluorine substances. Examples of suitable substances, giving the fluorine ion contained in U.S. patent No. 3535421 issued by Briner and other October 20, 1970, and U.S. patent No. 3678154 issued by Widder and other July 18, 1972 Illustrative ion sources of fluoride include tin fluoride, sodium fluoride, potassium fluoride, monitoroff sodium and many others. The tin fluoride and sodium fluoride are particularly preferred, and mixtures thereof.

Active agents, whitening teeth, and substances that modify the color of the teeth

Active agents, whitening the teeth, which can be used in compositions for caring for the oral cavity according to the present invention include bleaching or oxidizing agents such as peroxides, perborates, percarbonates, peroxyacids, persulfates, chlorites metals, and combinations thereof. Suitable peroxide compounds include hydrogen peroxide, urea peroxide, calcium peroxide, and mixtures thereof. The preferred percarbonate is percarbonate sodium. Other suitable active agents, whitening teeth, include persulfates, potassium, ammonium, sodium and lithium, mono - and tetrahydrate perborate and peroksigidrat pyrophosphate sodium. Suitable metal chlorites include chlorite calcium, barium chlorite, magnesium chlorite, chlorine is t lithium the sodium chlorite and potassium chlorite. The preferred chlorite is sodium chlorite. Additional whitening agents can be represented as hypochlorite and chlorine dioxide.

In addition to the bleaching agents as giving white teeth agents as active additives to care for the oral cavity, which can be used in the present invention, it is possible to consider the matter of modifying the color of the teeth. These substances are suitable for modifying the color of teeth to satisfy the consumer. These substances include particles, which when applied to the surface of the teeth modify the surface in terms of absorption or reflection of light. Such particles provide advantages appearance, when a film containing such particles, deposited on the surface of the tooth or teeth.

Particles are most useful in the present invention include pigments and dyes, commonly used in cosmetics. There are no specific limitations to the pigment or dye used in the present compositions, in addition to limiting effect caused by the light source when the illumination of the surface of the teeth. Pigments and dyes include inorganic white pigments, inorganic colored pigments, pearlescent agents, filling powders and the like. Spiral the examples selected from the group including talc, mica, magnesium carbonate, calcium carbonate, magnesium silicate, aluminumagnesium, silicon dioxide, titanium dioxide, zinc oxide, red iron oxide, brown iron oxide, yellow iron oxide, black iron oxide, salesonlinevyv ferrocyanide, manganese violet, ultramarine, nylon powder, polyethylene powder, methacrylate powder, powder of polystyrene, silk powder, crystalline cellulose, starch, titanate mica containing iron oxide titanate mica, bismuth oxychloride, and mixtures thereof. Most preferred are substances selected from the group consisting of titanium dioxide, oxychloride bismuth, zinc oxide and mixtures thereof. Pigments that are generally regarded as safe, are listed in the C.T.F.A. Cosmetic Ingredient Handbook, 3 rd Ed., Cosmetic and Fragrance Assn., Inc., Washington D.C. (1982).

Pigments are generally used as imparting opacity agents, and dyes. These pigments can be used in the form of processed particles or in the form themselves untreated pigments. Typical levels of pigments chosen for a specific action that you want the consumer. For example, for teeth that are particularly dark or colored, usually will make use of the pigments in quantities sufficient to lighten the teeth. On the other hand, when individual teeth or stains on the teeth with Atlee, than the other teeth, it is possible to use pigments that make the teeth darker. The levels of pigments and dyes are generally used in the range from about 0.05 to about 2%, preferably from about 0.10 to about 15% and most preferably from about 0.25 to about 10% by weight of the composition.

Humidifiers

Another optional component of the external oral carriers of the compositions according to the invention is a humidifier. The humectant serves to prevent toothpaste from hardening under the action of air, giving the feeling of moisture in the mouth, for certain humidifiers to impart desirable sweetness of the taste of the toothpaste compositions. Humidifier, based on a net basis humidifier, typically comprises from about 0 to about 70%, preferably from about 5 to about 25% by weight by weight of the composition. Suitable humectants for use in compositions according to the invention include food polynuclear alcohols, such as glycerin, sorbitol, xylitol, butyleneglycol, polyethylene glycol and propylene glycol, in particular sorbitol and glycerin.

Bicarbonate salt of an alkali metal

The present invention may also include a bicarbonate salt of an alkali metal. Bicarbonate salts of alkali metals are soluble in water and, if they are not stable, tend wisweb is to give carbon dioxide in the aqueous system. Sodium bicarbonate also known as baking soda, bicarbonate is the preferred alkali metal salt. The present composition may contain from about 0.5 to about 30%, preferably from about 0.5 to about 15% and most preferably from about 0.5 to about 5% bicarbonate salt of an alkali metal.

Mixed media

Water used in the preparation of commercially suitable compositions for the oral cavity, preferably should have a low content of ions and must not contain organic impurities. Water is usually from about 10 to about 50% and preferably from about 20 to about 40% by weight of the water in the toothpaste composition in accordance with the description. Such amounts of water include the free water which has been added, plus the water, which is introduced with other materials, such as with sorbitol.

Titanium dioxide may also be added in this composition. Titanium dioxide is a white powder, which adds the opacity of the composition. Titanium dioxide typically includes from about 0.25 to about 5% by weight of the composition of the tooth powder or tooth paste.

Other optional agents that can be used in these compositions include Dimethicone copolyol selected from alkyl - and alkoxyimino with the of aiolou, such as C12 and C20 alkyldiethanolamine copolyol and mixtures thereof. Vysokoproizvoditelnykh is acidimetry copolyol supplied to the market under the trade name Abil EM90. Dimeticone copolyol usually present at a level from about 0.01 to about 25%. Preferably from about 0.1 to about 5%, more preferably from about 0.5 to about 1.5% by weight. Dimeticone copolyol help to ensure a positive impact on the teeth.

Preservatives and pH-buffers

The preservatives and antimicrobial agents that can be used in toothpaste or gels include selected from the group consisting of p-hydroxybenzoic acid; the methyl, ethyl or propyl ether complex; sorbate sodium; sodium benzoate, bremgarten, esters phenylsalicylate acid, thymol, and the like and combinations thereof.

the pH of the present compositions preferably regulate through the use of buffering agents. The term "buffering agent"as used herein, refers to agents that can be used to regulate the pH of the compositions in the range of from about 4.5 to about a 9.5. Buffer agents include mononitrite, trisodium phosphate, sodium hydroxide, sodium carbonate, acidic sodium pyrophosphate, citric acid and sodium citrate. Buffering agents can be introduced at the level of p is IMEMO 0.5 to about 10% by weight of the present compositions. the pH of the compositions tooth powder or tooth paste is measured for aqueous suspension tooth powder or tooth paste in the ratio 3:1, for example 3 parts water to 1 part toothpaste. Suitable pH buffers include selected from the group consisting of primary, secondary or tertiary phosphates of alkali metals, citric acid, sodium citrate and the like or combinations thereof. Healing wounds and inhibiting inflammatory substances include selected from the group consisting of allantoin, urea, azulene, the active substances of chamomile and derivatives of acetylsalicylic acid and the like or combinations thereof.

Dyes

In the present invention can use food grade or pharmaceutically acceptable coloring agents or pigments that will be clear to the person skilled in the art. An example of a pigment to provide a bright white color is titanium dioxide (such as U.S.P. quality, available from Whittaker, Clark &Daniels). As will be clear to the person skilled in the art, these compositions can be used food grade or pharmaceutically acceptable coloring agents, pigments or dyes, including FD&C pigments, primarily FD&C blue No. 1, FD&C blue No. 2, FD&C green No. 3, FD&C yellow No. 5, FD&C yellow No. 6, FD&C red No. 3, FD&C red No. 33 and FD&C red No. 40 and reddish pigments FD& C blue No. 1, FD&C blue No. 2, FD&C yellow No. 5, FD&C yellow No. 6, FD&C red No. 2, FD&C red No. 3, FD&C red No. 33, FD&C red No. 40, and combinations thereof. Can be used as pigments and dyes. The composition preferably contains from about 0.1 to about 10% of these agents, preferably from about 0.1 to about 1% by weight of composition.

Typical liquid composition for the oral cavity, rinse for the mouth, spray to the oral cavity, chewing gum and mints will include from about 30 to about 80% water, from about 2 to about 35% of humectant, from about 1 to about 50% of the active compounds comprising at least Morinda citrifolia, from about 0.01 to about 0.50% is at least one of a sweetener, from about 0.01 to about 0.50% is at least one thickener or filler, which can be dispersed in from about 2.5 to about 10% of the medium, such as glycerin, polyethylene glycol (PEG-400) or their combinations, from about 0.03 to about 3% of at least one surfactant and from about 0.01 to about 1% of at least one flavoring. Typical liquid composition for the oral cavity, rinse for the mouth, spray to the oral cavity, chewing gum and mints may not necessarily include from about 0.01 to about 1.0% of a dye substance, to the which includes dyes and pigments, and from about 0.01 to about 1.0 percent of the agents, giving the opacity. The composition may further include from about 0.01 to about 1.0% titanium dioxide.

In another embodiment of the invention, the composition for the care of the oral cavity may be in the form of tools for cleaning teeth, such as toothpaste or gels. It is usually assumed that toothpastes and gels are pasty or gel-like preparations, which are applied directly to the teeth, usually with the help of a toothbrush, and a means for cleaning the teeth can be a combination of pastes and gels, as well as a combination of gel or toothpaste with liquids for oral or mouthwash for mouth. Chewing gum and mints can also be used as a means for brushing your teeth, provided that they include active ingredients that are commonly associated with compositions for cleaning teeth.

Composition for cleaning teeth will usually contain from about 5 to about 20% water, from about 5 to about 75% of humectant, from about 0.25 to about 10% of at least one thickener or filler, which can be dispersed in from about 2.5 to about 10% of media, such as glycerin, polyethylene glycol (PEG-400) or their combinations, from about 0.01 to about 0.05% sweeteners, from about 5 to primer is 40% abrasive and polishing substances, from about 0.5 to about 3% surfactant, from about 0.01 to about 50.0% of active compounds, includingMorinda citrifoliaand which may contain active hygienic substances to the mouth, antibacterial agents, lowering the sensitivity of agents, agents against the formation of plaque and combinations thereof, and from about 0.25 to about 3.0 percent of flavors. Composition for cleaning teeth, can also include fillers and adjuvants, for example, from about 0.05 to about 1.0% of a preservative and/or antimicrobial agent, from about 0.50 to about 10.0% of buffers, from about 0.05 to about 5.0% of wound healing and inhibiting inflammation substances, from about 0.01 to about 2.0% of the coloring matter such as pigments, dyes or particles for special effect, and from about 0.05 to about 10.0 percent of bleaching agents such as hydrogen peroxide and pyrophosphates. Below are several embodiments of the compositions. However, they are intended only for illustration, as an ordinary person skilled in the art will be apparent other compounds or compositions, including recycled productMorinda citrifolia.

In one illustrative embodiment, an additional distinctive feature of the present invention is a method of introducing pisew the th song includingMorinda citrifoliato care for the oral cavity. The method includes the following stages: (a) the creation of a food composition, including partly processed productMorinda citrifoliapresent in amounts of between about 0.01 and 95% by weight, where the composition also includes a carrier, such as water or purified water, and other natural or artificial ingredients; (b) introduction of food composition in the organism, so that the processed productMorinda citrifoliaabsorbed in sufficient degree; (C) repeating the above stages as often as necessary to ensure the effective number of processed productMorinda citrifolia.

Stage of introduction of food composition in the body includes oral ingestion of the composition by one of several ways. Specifically, the food composition may be made in the form of liquid, gel, solid, or several other varieties, which will allow you to quickly and easily digest it, to chew, or to influence other way on tissue present in the mouth of a mammal. In addition, revisedMorinda citrifoliamay be included in the toothpaste or gel, powder, granules, dezintegriruetsja tablets, liquid oral, lozenges or chewing gum.

These pills illustrate or represent the some of the preferred compounds or compositions, considered in this invention. As indicated, they are presented only as illustrative embodiments of, and are not intended to limit in any way.

Table 1.

Tooth powder, granules or dezintegriruetsja tablets
IngredientWeight %
Calcium carbonate50,0
Morinda citrifolia31,0
Microcrystalline cellulose14,6
Pluronic F1212,0
Xanthan gum1,0
Methocel K15MP0,5
Flavor0,5
GTerminal ammonium0,4

Another variant of implementation of the present invention provide a tool for brushing in the paste. A typical formula toothpaste in accordance with the claims of this invention are shown in table 2.

Table 2.

Toothpaste
IngredientWeight %
Morinda citrifolia35,9
Waterthe 33.4
carbonat calcium 24,1
Pluronic4,0
Cellulose gum1,5
Methocel0,5
Glycyrrhizinate of dItalia0,4
Flavor0,2

One of the variants of the present invention is a means for brushing your teeth in the form of a gel. A typical formula is given in table 3.

Table 3.

Dental gel
IngredientWeight %
Morinda citrifolia38,0
Water33,3
Thickener silicon dioxide10
Glycerin7,3
The abrasive is silicon dioxide5
Pluronic4
Cellulose gum1,5
Methocel0,5
Glycyrrhizinate of dItalia0,2
Flavor0,2

One of the embodiments of the invention is a solid or soft toffee, preventing the formation of plaque for sucking the consumer. A typical formula for the lozenges according to Faure who uloi of the invention are shown in table 4.

Table 4.

The toffee for brushing
IngredientWeight %
Morinda citrifoliato 91.6
Pluronic4,0
Cellulose gum1,0
Methocel0,5
Calcium carbonate2,0
Flavor0,5
Glycyrrhizinate of dItalia0,4

One of the embodiments of the invention is a chewing gum that prevents the formation of plaque. A typical formula for chewing gum in accordance with the invention are listed in table 5.

Table 5.

Chewing gum for teeth brushing
IngredientWeight %
The basis of chewing gum20,0
Morinda citrifolia67,5
Calcium carbonate5,0
Glycerin3,0
Pluronic2,0
Cellulose gum1,0
Methocel0,5
Flavor0,5
Glycyrrhizinate of monoamine0,4
Xanthan gum0,1

One of the embodiments of the invention is a liquid for oral cavity, preventing the formation of plaque. A typical formula of liquid to the oral cavity are listed in table 6.

Table 6.

Liquid oral
IngredientWeight %
Water65,49
Morinda citrifolia32,1
Pluronic1,0
Cellulose gum0,24
Methocel0,12
Flavor0,5
Glycyrrhizinate disodium0,4
Preservative0,1
Sodium fluoride0,05

In one preferred method of person who wants to take care of the oral cavity, as described above, accept, or enter at least one ounce of liquid to the oral cavity (shown in table 6) early in the morning and at least one ounce in the evening before you go to bed. In one example, which is not implied is provided as limiting in any way, providing beneficial effectsMorinda citrifoliaprocessed into juice Tahitian Noni®produced by Morinda Incorporated in Orem, Utah.

In another preferred method according to the present invention a person who wants to take care of the oral cavity, as described above, applies the above toothpaste using a cleaning mechanism for applying the paste on the teeth. After cleaning using a toothpaste according to the present invention the person holds at least one ounce of liquid for oral cavity according to the present invention in the mouth for a sufficient period of time. In a preferred embodiment of the present invention, this method is repeated many times throughout the day, including early morning, before bed and after meals. These specific methods of administration of the composition may include any effective way of introducing the composition to the patient to care for the oral cavity. Although there are many ways, in the present invention it is clear that the composition can be administered in a number of mechanisms, including oral administration. No matter how the method is used, it is important to regulate the amount of active ingredient which a patient acts in such a way that carried out properly the tasks of caring for the oral cavity. In the following examples, the decree is about and presents the action to care for the oral cavity with the use of Morinda citrifolia. These examples are not intended to limit the invention in any way, and are only an illustration of the advantages and benefits of the use ofMorinda citrifoliato care for your mouth.

3. Stimulation of care for the oral cavity

The present invention may be embodied in other specific forms without departing from its essence and essential features. Described embodiments of all aspects are considered only as illustrative, and not restrictive. Scope of the invention, therefore, is specified in the accompanying claims, and not by the description above. All changes that occur within the meaning and range of equivalents of the claims covered by this volume.

The present invention uses these properties by introducing components of the plantMorinda citrifoliaas components of compositions for oral care mouth for the treatment and prevention of various disorders of the oral cavity and associated dental disorders. Some embodiments of the present invention include a core component of excipient and active component that includesMorinda citrifoliaup to fifty percent by weight.

Example 1. Prevention of gum disease

Taking into consideration the above, the task some options about what westline of the present invention is to create a dental product for the oral cavity, can cause incremental immune response to gum disease or tooth decay. In this embodiment of the present invention a person who wants to take care of the oral cavity, as described above, applies the above toothpaste using a cleaning mechanism for applying the paste on the teeth. After cleaning using a toothpaste according to the present invention of man holds in his mouth at least one ounce of the above-described liquid to the oral cavity for a period of time sufficient to inhibit gum disease or tooth decay. In a preferred embodiment of the present invention, this method is repeated many times throughout the day, including early morning, before bed and after meals. These specific methods of administration of the composition may include any effective way of introducing the composition to the patient to care for the oral cavity, including the use of the above-described tooth powder, granules, dezintegriruetsja tablets, paste, gel, lozenges, chewing gum, or liquid for oral separately or in combination with each other. No matter how the method is used, it is important to regulate the amount of active ingredient which a patient acts in such a way that was carried out in a suitable manner for the ACI care for the oral cavity.

Example 2. Increase in the density of collagen

Another objective of certain embodiments of the present invention is to provide a dental product for the oral cavity and method of reducing bleeding gums by reducing inflammation in the hollows of the gums and increase the density of collagen in the gums. In this embodiment of the present invention a person who wants to take care of the oral cavity, as described above, applies the above toothpaste using a cleaning mechanism for applying the paste on the teeth. After cleaning using a toothpaste according to the present invention of man holds in his mouth at least one ounce of the above-described liquid to the oral cavity for a period of time sufficient to increase the density of collagen in the gums. In a preferred embodiment of the present invention, this method is repeated many times throughout the day, including early morning, before bed and after meals. These specific methods of administration of the composition may include any effective way of introducing the composition to the patient to care for the oral cavity, including the use of the above-described tooth powder, granules, dezintegriruetsja tablets, paste, gel, lozenges, chewing gum, or liquid for oral cavity according to the Department of the property or in combination with each other. No matter how the method is used, it is important to regulate the amount of active ingredient which a patient acts in such a way that carried out properly the tasks of caring for your mouth.

Example 3. Reducing plaque

Another objective of certain embodiments of the present invention is to provide a dental product for the oral cavity and of the method of reducing loss of periodontal accession by reducing plaque formation and/or adhesion of plaque to the teeth and surrounding tissues of the oral cavity. In this embodiment of the present invention a person who wants to take care of the oral cavity, as described above, applies the above toothpaste using a cleaning mechanism for applying the paste on the teeth. After cleaning using a toothpaste according to the present invention of man holds in his mouth at least one ounce of the above-described liquid to the oral cavity for a period of time sufficient to reduce plaque formation and/or adhesion of plaque to the teeth and surrounding tissues of the oral cavity. In a preferred embodiment of the present invention, this method is repeated many times throughout the day, including early morning, before bed and after meals. These specific STRs is usually used for injection of the composition may include any effective way of introducing the composition to the patient to care for the oral cavity, including the use of the above-described tooth powder, granules, dezintegriruetsja tablets, paste, gel, lozenges, chewing gum, or liquid for oral separately or in combination with each other. No matter how the method is used, it is important to regulate the amount of active ingredient which a patient acts in such a way that carried out properly the tasks of caring for your mouth.

Example 4. Reducing bacterial activity

Another objective of certain embodiments of the present invention is to provide a dental product for the oral cavity and method of reducing the permeability of cell walls, bacterial toxins, and additionally to reduce collagenase enzymatic activity in the presence of bacteria. In this embodiment of the present invention a person who wants to take care of the oral cavity, as described above, applies the above toothpaste using a cleaning mechanism for applying the paste on the teeth. After cleaning using a toothpaste according to the present invention of man holds in his mouth at least one ounce of the above-described liquid to the oral cavity for a period of time sufficient to reduce collagenase enzymatic activity in the presence of bacteria. In p edocfile embodiment of the present invention, this method is repeated many times during the day, including early morning, before bed and after meals. These specific methods of administration of the composition may include any effective way of introducing the composition to the patient to care for the oral cavity, including the use of the above-described tooth powder, granules, dezintegriruetsja tablets, paste, gel, lozenges, chewing gum, or liquid for oral separately or in combination with each other. No matter how the method is used, it is important to regulate the amount of active ingredient which a patient acts in such a way that carried out properly the tasks of caring for your mouth.

Example 5. The increase of cellular respiration

Another objective of certain embodiments of the present invention is to provide a dental product for the oral cavity and method for increasing cellular respiration and oxygen saturation of the cells of the mouth. In this embodiment of the present invention a person who wants to take care of the oral cavity, as described above, applies the above toothpaste using a cleaning mechanism for applying the paste on the teeth. After cleaning using a toothpaste according to the present invention of man holds in his mouth at least one ounce of the above-described liquid to the oral cavity during the period of time, sufficient to increase cellular respiration and oxygen saturation of the cells of the mouth. In a preferred embodiment of the present invention, this method is repeated many times throughout the day, including early morning, before bed and after meals. These specific methods of administration of the composition may include any effective way of introducing the composition to the patient to care for the oral cavity, including the use of the above-described tooth powder, granules, dezintegriruetsja tablets, paste, gel, lozenges, chewing gum, or liquid for oral separately or in combination with each other. No matter how the method is used, it is important to regulate the amount of active ingredient which a patient acts in such a way that carried out properly the tasks of caring for your mouth.

Example 6. Inhibition of ulceration of the oral cavity

The next challenge some embodiments of the present invention is to provide a dental product for the oral cavity and method for reducing the frequency and duration of ulceration of the oral cavity. In this embodiment of the present invention a person who wants to take care of the oral cavity, as described above, applies the above toothpaste using the Chi is shining, the mechanism for applying the paste on the teeth. After cleaning using a toothpaste according to the present invention of man holds in his mouth at least one ounce of the above-described liquid to the oral cavity for a period of time sufficient to reduce the frequency and duration of ulceration of the oral cavity. In a preferred embodiment of the present invention, this method is repeated many times throughout the day, including early morning, before bed and after meals. These specific methods of administration of the composition may include any effective way of introducing the composition to the patient to care for the oral cavity, including the use of the above-described tooth powder, granules, dezintegriruetsja tablets, paste, gel, lozenges, chewing gum, or liquid for oral separately or in combination with each other. No matter how the method is used, it is important to regulate the amount of active ingredient which a patient acts in such a way that carried out properly the tasks of caring for your mouth.

Example 7. Remove plaque

Another objective of certain embodiments of the present invention is to create a dental product for the oral cavity and method of cleaning teeth, removing plaque from the teeth and teeth whitening. In this variant the implementation of the present invention of man, who wants to take care of the oral cavity, as described above, applies the above toothpaste using a cleaning mechanism for applying the paste on the teeth. After cleaning using a toothpaste according to the present invention of man holds in his mouth at least one ounce of the above-described liquid to the oral cavity for a period of time sufficient to clean your teeth, removing plaque from the teeth and teeth whitening. In a preferred embodiment of the present invention, this method is repeated many times throughout the day, including early morning, before bed and after meals. These specific methods of administration of the composition may include any effective way of introducing the composition to the patient to care for the oral cavity, including the use of the above-described tooth powder, granules, dezintegriruetsja tablets, paste, gel, lozenges, chewing gum, or liquid for oral separately or in combination with each other. No matter how the method is used, it is important to regulate the amount of active ingredient which a patient acts in such a way that carried out properly the tasks of caring for your mouth.

Example 8. Improving dental health

Another objective of certain embodiments of the present from which bretania is to create a dental product for the oral cavity and method of treatment and prevention of gum disease and tooth decay in mammals and in addition to the overall improvement of the cavity mouth in General. In this embodiment of the present invention a person who wants to take care of the oral cavity, as described above, applies the above toothpaste using a cleaning mechanism for applying the paste on the teeth. After cleaning using a toothpaste according to the present invention of man holds in his mouth at least one ounce of the above-described liquid to the oral cavity for a period of time sufficient for the treatment and prevention of gum disease and tooth decay in mammals and in addition to the overall improvement of oral health in General. In a preferred embodiment of the present invention, this method is repeated many times throughout the day, including early morning, before bed and after meals. These specific methods of administration of the composition may include any effective way of introducing the composition to the patient to care for the oral cavity, including the use of the above-described tooth powder, granules, dezintegriruetsja tablets, paste, gel, lozenges, chewing gum, or liquid for oral separately or in combination with each other. No matter how the method is used, it is important to regulate the amount of active ingredient which a patient acts in such a way as to suitably carried out the way the tasks of caring for your mouth.

Example 9. Reducing bad breath

The next challenge some embodiments of the present invention is to provide a composition for the oral cavity and the way to reduce bad breath in mammals. In this embodiment of the present invention a person who wants to take care of the oral cavity, as described above, uses the above-described toothpaste, using a cleaning mechanism for applying the paste on the teeth. After cleaning toothpaste of the present invention the person holds at least one ounce of the above-mentioned liquid to the mouth in the mouth over a period of time sufficient to reduce bad breath in mammals. In a preferred embodiment of the present invention, this method is repeated many times throughout the day, including early morning, before bed and after meals. These specific methods of administration of the composition may include any effective way of introducing the composition to the patient to care for the oral cavity, including the use of the above-described tooth powder, granules, dezintegriruetsja tablets, paste, gel, lozenges, chewing gum or rinse for the mouth separately or in combination with each other. No matter which method is used, it is important that regulation if the ESCWA active ingredient, which affects the patient so that was carried out appropriate tasks of caring for your mouth.

Example 10. Antimicrobial properties of recycled productsMorinda citrifolia

Research conducted for the reinforcement of the present invention demonstrate that recycled productsMorinda citrifoliapossess antimicrobial activity. Minimum inhibitory concentration (MIC) of the bactericide is defined as the maximum dilution of the product, which still inhibits growth of the test microorganism. The minimum lethal concentration (MLC) of the bactericide is defined as the maximum dilution of the product that will kill the test organism. The values of MIC/MLC can be defined by a set of standard methods of testing. The most common used methods are the method of cultivation in vitro and method of dilution in agar. The method of cultivation in vitro was proposed for this product to determine the MIC, and aliquots in Petri dishes from dilutions showed the best inhibition of growth to determine the MLC. Serial dilution was carried out for the products in the environment for bacterial growth. The test organisms were added to the cultivation of products, incubated and were counting their growth. All tests were conducted three times again the minute.

This procedure is a standard antimicrobial test. The method includes the content and intentions of a methodology recommended by the American microbiological society (ASM). In the method of cultivation in vitro use dilution of the test product in an environment for bacterial growth, insulinopenia using a predetermined concentration of the test organism and visualization of growth after incubation. The methods of cultivation in the tubes is limited to products that do not precipitate or form of turbidity in the medium for growth within the expected target range.

Cell cultures were obtained from the original solution, the test organisms was transferred into a digested soybean casein broth (SCOB), and incubated at 37±2°With in 24-48 hours for bacteria and at 20-25°for yeast. If necessary, the suspension was brought to approximately 108 colony forming units (CFU) per ml by visual density in physiological solution (PHSS) and was performed by standard counting on the planets to determine the source of the title. The yeast culture was placed on dextrose-Sabouraud's agar (SDEX) and incubated at 20-25°C for 2-4 days, S.mutans incubated at 37±2°C for 3-5 days and all other bacteria were incubated at 37±2°C for 18-24 hours.

The tests were carried out at neutral the pH nom. The pH values were recorded before and after the delivery. Each test product was serially diluted 1:2 in sterile water. Chose cultivation, which should demonstrate the endpoint MIC/MLC. The evaluation of each test product was performed in triplicate for each organism. Dilution of the product was added to equal volume of 2X SCDB for additional dilution 1:2.

For each test organism got three tubes of the positive control by mixing sterile water with an equal volume of 2X SCDB. Three tubes of the negative control was prepared by mixing the highest tested cultivation of the investigated product with equal volumes of 2X SCDB. Three tubes of the control medium was prepared by mixing sterile water with an equal volume of 2X SCDB. In these tubes the test organisms were not added.

Approximately 0.05 ml suspension of each test organism was added to the sample in a test tube positive control. The tubes with the test bacteria were incubated at 37±2°C for 18-24 hours and tubes tested yeasts were incubated at 20-25°C for 2-4 days. After incubation, the growth was calculated as negative (0) or positive (+) to each tube.

Only tubes that were supposed lack is any growth, tested to determine the MLC. a 1.0 ml aliquot was removed from each tube and perform a serial dilution of 1/10 in neutralizing broth up to 1/1000. An aliquot of each dilution was placed on the agar Converter (NUAG). For positive control, 10-100 CFU was placed on NUAG. Negative control was obtained by placing 2 SCDB on NUAG. The cups were incubated at 20-25°C for 2-4 days for yeasts and at 37±2°C for 18-24 hours for all bacteria except S.mutans.

The lowest dilution tested product that has been tested against MLC, tested for the recovery of neutralization for each test organism. In triplicate 0.5 ml aliquots of the most concentrated products tested was placed on NUAG. Cups pinned 10-100 CFU of each of the test organisms. For comparison, three cups NUAG without the tested product also pinned the same 10-100 CFU of each of the test organisms.

With the exception of S.mutans all organisms inhibited neitralizovannykh Morinda citrifolia concentrate in a concentration of 1:2. None of the tested dilutions were not able to demonstrate the lethality for any organism. No inhibition or mortality has not been demonstrated neutralized by Noni concentrate when testing against S.mutans. The MIC results for all the of organizmov summarized in tables 7-13. The results of the MLC for each organism are summarized in tables 14-19. Because S.mutans not had any dilutions, as was assessed as not showing growth for the MIC part of the test, for a given organism definition MLC did not.

Restoration of neutralization for all tested organisms ranged 40-97%. Recovery neutralizing neutralizing medium used in this study are summarized in table 20.

Table 7: Results of MIC

Escherichia coli0157H7 ATCC # 43885
BreedingGrowth +/0
1:2000
1:4+++
1:8+++
1:16+++
1:32+++
1:64+++
Positive+++
Negative000
Wednesday000

Title 7,0×108CFU/ml

Table 8: Results of MIC

Staphylococcus aureusATCC # 6538
BreedingGrowth +/0
1:2000
1:4+++
1:8+++
1:16+++
1:32+++
1:64+++
Positive+++
Negative000
Wednesday000

The titer of 6.5×108CFU/ml

Table 9: Results of MIC

Bacillus subtilisATCC # 19659
BreedingGrowth +/0
1:2000
1:4+++
1:8+++
1:16+++
1:32+ ++
1:64+++
Positive+++
Negative000
Wednesday000

The titer of 8.5×107CFU/ml

Table 10: Results of MIC

Salmonella cholerauesuisserotypeenteritidisATCC # 13706
BreedingGrowth +/0
1:2000
1:4+++
1:8+++
1:16+++
1:32+++
1:64+++
Positive+++
Negative000
Wednesday000

Title 4,8×108CFU/ml

Tab the Itza 11: Results of MIC

Listeria monocytogenesATCC # 19111
BreedingGrowth +/0
1:2000
1:4+++
1:8+++
1:16+++
1:32+++
1:64+++
Positive+++
Negative000
Wednesday000

The titer of 3.9×108CFU/ml

Table 12: Results of MIC

Candida albicansATCC # 10231
BreedingGrowth +/0
1:2000
1:4+++
1:8+++
1:16+++
1:32+++
1:64 +++
Positive+++
Negative000
Wednesday000

The titer of 1.3×108CFU/ml

Table 13: Results of MIC

Streptococcus mutantsATCC # 25175
BreedingGrowth +/0
1:2+++
1:4+++
1:8+++
Positive+++
Negative000
Wednesday000

The titer of 1.0×108CFU/ml

Table 14. MLC counting tablet

Escherichia coli0157H7 ATCC # 43888
BreedingThe repetitionBreeding
10010-110-210-3
1:21TNTCTNTCTNTC245
2TNTCTNTCTNTC239
3TNTCTNTCTNTC215
Note: it is placed volume = 0,5 ml TNTC = too numerous to count

Table 15. MLC counting tablet

Staphylococcus aureusATCC # 6538
BreedingThe repetitionBreeding
10010-1102103
1:21TNTCTNTCTNTC200
2TNTCTNTCTNTC134
3TNTCTNTCTNTC114
TNTC = too numerous to count
Table 16. MLC counting tablet

Bacillus subtilisATCC # 19659
BreedingThe repetitionBreeding
10sup> 010-1102103
1:2127300
225200
318200
TNTC = too numerous to count
Table 17. MLC counting tablet

Salmonella cholerauesuisserotypeenteritidisATCC # 13706
BreedingThe repetitionBreeding
10010-1102103
1:21TNTCTNTC417
2TNTCTNTC755
3TNTCTNTC636
TNTC = too numerous to count
Table 18. MLC counting tablet

Listeria monocytogenesATCC # 19111
BreedingThe repetitionBreeding
10010-1102103
1:21TNTCTNTCTNTC109
2TNTCTNTCTNTC109
3TNTCTNTCTNTC179
TNTC = too numerous to count
Table 19. MLC counting tablet

Candida albicansATCC # 10231
BreedingThe repetitionBreeding
10010-1102103
1:21INTOTNTCTNTC168
2TNTCTNTCTNTC117
3TNTCTNTCTNTC138
TNTC = too numerous to count

Table 20. Neutralization
BodyA positive count Counting neutralizationThe percent recovery
123AVE123AVE
E. coli606358605350735997%
S.aureus486538504944424589%
B.subtilis536153562520222240%
S.choleraesuis364336393434313385%
L.monocytogenes433822342626343088%
C. albicans362521272020272072%
S.mutans11193 1499141391%

Example 11. RevisedMorinda citrifoliaas a dental medicines.

To assess the development of the use ofMorinda citrifoliaas a dental medicines can be carried out a simple scientific investigation. The present invention involves three areas of research that confirm the safety and efficacy ofMorinda citrifoliaas an agent to care for the oral cavity. First, a survey will be conducted to study the antimicrobial activity ofMorinda citrifoliain vitro versus conventional periodontal and endodontic pathogens. The second study will be performed to examine the efficacy ofMorinda citrifoliawhen cleaning and disinfection of the root canals of the tooth pulp. Thirdly, a survey will be conducted to assess the biocompatibility in vitroMorinda citrifoliain relation to oral tissues.

The antimicrobial effect irrigating agents tested using cultures in vitro conventional periodontal and endodontic pathogens. The most widely accepted and used method is the evaluation of test substances using a standardized method one disc. This method involves inoculation of discos test substances, in the case of the present invention withMorinda citrifoliato assess their antimicrobial activity. The advantage of using this standard method is connected with ease interpretation of antimicrobial activity and a high degree of attainable reproducibility. The effectiveness of the studied substances in relation to cleaning and disinfection of root canals can be evaluated using scanning electron microscopy to visualize the adhesion and removal of microbes. Remove microbes after endodontic therapy is important in order to avoid subsequent failure of treatment due to complications caused by the persistence of microbes. It is also important to remove any layer of dentin to improve the fastening of endodontic materials on the surface of the teeth. Inadequate fixation of endodontic materials can lead to infiltration of microbes in the tissues of the mouth, and this may cause complications during treatment, leading to failure. If it turns out that the tested substances have effective antimicrobial activity and is effective in cleaning and disinfection of root canals, they must be assessed for safety, because they can be used as part of dental treatment. Toxicity potential medicines investigated using tests to measure Biokovo is Timothy. The interaction between tissues and substances called biocompatibility. Biocompatibility is measured in accordance with the evaluation criteria defined by the International organization for standardization (ISO) and the International dental Federation. These organizations developed directives ISO 10993 and 7405, which specify specifically preclinical tests for devices and materials used as part of dental treatment. Dental materials that undergo preclinical tests ISO can be used all over the world, because the standards include local and national standards. The standards include requirements Management on sanitary inspection behind quality of foodstuff and medicines of the American dental Association.

Ideal irrigating solution should possess antimicrobial activity, low toxicity and good biocompatibility with tissue of the oral cavity and be able to clean the walls of the root canal and remove formed by a layer of sediments. Layer formed of sediments is a denatured finely divided debris thickness of 1 mm formed on instrumental processed surfaces of the cavity, and is composed of dentin, odontodactylus products, non-organic zagryaznenii microorganisms. Removing the layer formed deposits with treated walls of the root canal is controversial. Its removal provides the best adhesion materials-fillers to dentin and to exclude the passage of microorganisms in the tissues of the mouth. Infiltration of microorganisms in the tissues of the mouth should be avoided because they often cause complications leading to treatment failure.

1. Study of antimicrobial activity of Morinda citrifolia in vitro against conventional periodontal and endodontic pathogens

The antimicrobial effectMorinda citrifoliait was not until this point been tested on normal periodontal and endodontic pathogens. The purpose of this part of the application is to assess the antimicrobial activity ofMorinda citrifoliain vitro against seven normally allocated microbiological pathogens of the oral cavity. Isolates specific microbes will be obtained from the American type culture collection (ATSS). Pure cultures will be stored in 1.5 ml closed glass vials at a temperature of -68°to -72°C. After removal of data vials from the freezer leave them to thaw before inoculation into sterile test tubes containing the medium broth for growth. The suspension will be shaken in a vortex mixer and incubate for 3-5 days at 37°C in an anaerobic chamber Koya containing the tmosferu, consisting of 85% nitrogen, 10% hydrogen and 5% carbon dioxide. For all types of broths one control sample (without adding inoculum) will be processed in the same way as the inoculated tubes to ensure sterility.

After a period of inoculation purity of each culture on stage broth cultures will be evaluated by the method ramakrisha microscopy before inoculation on agar plates. After shaking cultures using a vortex mixer will conduct inoculation on agar tablets on the blood of sheep by moistening a sterile cotton swabs in cultures that remove excess inoculum by pressing the pad on the opposite side of the test tube, and then distribute the inoculum evenly over the entire agar surface. Tablets leave to air dry for 2 to 3 minutes.

Susceptibility of different microorganisms toMorinda citrifoliawill be assessed using the method of standardized single disk, similarly used to test the sensitivity to antibiotics. Sterile blank discs carefully placed on the agar surface using sterile techniques. The disks then load 10 ál of different concentrations ofMorinda citrifolia. Clindamycine disks will be used as polojitelnogo the control and sterile disks with sterile saline instead of Morinda citrifoliawill be used as a negative control. The disks will be in spatial terms placed on the surface covered by the blood agar as far as possible from each other (about 3 cm), while maintaining a distance of at least 2 cm from the ends of the Petri dishes. The time elapsed between the destruction of the culture of the anaerobic chamber, the distribution of the inoculum, the placement drives and load different experimental treatments, estimated at 15 minutes. For each species of microbes will be received five replicates of three spatially separated disks on agar tablets (15 tablets on the bacterial species). A concentrated solutionMorinda citrifoliawill be divorced in 10-fold concentrations (dosage; 1x, 10x, 100x and 1000x) and loaded onto blank disks. the pH of the dilutionMorinda citrifoliawill be adjusted so that it was identical to the pH of the initial solution (e.g., pH 8,9)

Sown plates will be incubated for 5-7 days in an anaerobic chamber. Zones of inhibition produced by various concentrations ofMorinda citrifoliawill be measured using calipers. Registration 7 mm (diameter of the disk) indicates no zone of inhibition of bacterial growth. The outer boundary of the zone of inhibition will be determined as the most remote area from the border of the disk, where fully the inhibition of bacterial growth. If you attempt to evaluate the minimum inhibitory concentrationMorinda citrifoliafor each of the 6 species of microbes, intermediate doseMorinda citrifoliawill be calculated from the concentrations that give a large zone of inhibition, and those that do not inhibit bacterial growth. Will examine five of these inhibitory doses.

All data will be analyzed using statistical test one-way analysis of variants (ANOVA) with subsequent post test for multiple pairwise comparisons at a significance level of P<0,05. This statistical method is the most conservative and reliable among the methods available for the analysis of the original data.

2. Study of the effectiveness of Morinda citrifolia for cleaning and disinfection of root canals

Human teeth will be obtained from clinics with the approval of the Internal Commission for supervision. Will be selected sixty viewgallery intact permanent teeth that have not been stored in antibacterial or fixing solutions and have not been root canal treatment. All teeth have a single root canal and the length of the channels will range from 12 to 16 mm will Be received accepted available drugs and root canals will handle at a distance of 1 mm from the vertex of the pass using files of Hedstrom (Hedstroem) on the 50 size. During processing will be conducted by irrigation with sterile water. After preparation of the root canal extended vertex passage will close with epoxy resin to prevent the ingress of bacteria. In order to facilitate the handling and identification of teeth will be installed vertically in plastic blocks and sterilized in the autoclave for 20 minutes at 121°C.

For infection of root canals in remote teeth will be using pure culture ofEnterococcus fecalisATTCC 29212 grown in the Cerebro-heart infusion broth. Root canals will be inoculated with 10 FL suspension of 1.5×108CFU/ml using sterile 1 ml tuberculin syringes. Plastic blocks will be placed inside boxes of stainless steel and incubate at 37°C for 24 hours. After incubation, infected teeth will be divided into six groups according to the irrigation regimes: (1) teeth (n10) c root canals irrigated with 2 ml ofMorinda citrifoliausing the optimum concentration determined in phase 1 of this application; (2) teeth (n10) c root canals irrigated with 2 ml of 2%chlorhexidine solution; (3) teeth (n10) c root canals irrigated with 2 ml of 6%sodium hypochlorite solution; (4) teeth (n10) c root canals irrigated with 2 ml of 0.9%sterile fiziologicheskogo the solution as a processing negative control; (5) the teeth (n10) c root canals irrigated with 2 ml of a mixture ofMorinda citrifoliaand 2% chlorhexidine; (6) teeth (n10) c root canals irrigated with 2 ml of a mixture ofMorinda citrifoliaand 6% sodium hypochlorite solution.

6 irrigations (discussed above) will be conducted using a 2 ml sterile plastic syringe and needle 24 size. The needle will be inserted in the root canal is approximately 1 mm from the walls and 2 ml of irrigating fluid will get into the channel. Irrigating solution will be injected into the channel within 5 minutes and be removed from the channel by spraying 1 ml of sterile physiological solution.

The effectiveness of 6 variants of the irrigation treatment for cleaning and disinfection of the root canals will be assessed using micrographic images of the root canals, high power, obtained using scanning electron devices (SEM). The teeth will be prepared for use in SEM by fixing the dental tissues in 10%formalin solution at 18°C for 24 hours. The teeth will then be postvictorian in osmium tetroxide (1% V/V) for 2 hours before were dehydrated in a ranked series of ethanol solutions of(20%, 50%, 70%, 90%, for 15 minutes each, followed by treatment 3×10 minutes in 100% ethanol). Then they will be removed from solution and placed in hexamethyldisilazane 5 minutes of graphicsati digidrirovanny samples. Sections of the teeth are dried on filter paper dry. The dried samples will be posted on aluminum stereocamera small stands with or conductive carbon cement or quickly grasping Araldite. Dried fixed-base samples will be covered 20-30 mm thin metal layer of gold/palladium in a device for coating by the method of spraying Poiaron E5000 and assessed in Quita 200 SEM. SEM micrograph will be obtained when the ×2000 increase with the use of the software analysis of digital images. Will explore all 60 samples (each split into two) and micrographic images will be stored as digital files in the computer Acer, which is connected with the SEM. Each of the root canals will be scanned in its entirety to get a General idea of the topography of the surface. Micrography will be made for illustrative areas typical of the General topography of the surface of each sample. Detinova the surface of the root canals will be assessed for the presence of a layer of sediments and microorganisms using a semiquantitative visual criterion using a scale from zero to four.

The effectiveness ofMorinda citrifoliaand the irrigation treatment for cleaning and disinfection of the root canals will be assessed the BL is using semiquantitative criteria:

0: no removal of microorganisms and sediment layer from the root canal.

1: less than 50% removal of microorganisms and sediment layer.

2: approximately 50% removal of microorganisms and sediment layer.

3: more than 50% removal of microorganisms and sediment layer.

4: complete removal of microorganisms and sediment layer.

3. Evaluation of in vitro biocompatibility Morinda citrifolia with the tissues of the mouth.

Toxicological studies were conducted on rats to assess acute toxicityMorinda citrifolia. Using the probe was introduced fifteen thousand mg/kg Animals were observed for 14 days after treatment. All animals survived, and adverse signs were observed. After necropsy signs of General toxicity were observed.

To assess risk of allergicMorinda citrifoliatwo surveys have been conducted on Guinea pigs. Both studies included phase induction and rest period followed by the introduction ofMorinda citrifolia. The first study included two test groups of six animals in each group, positive control and negative control group. After injection, animals were observed for 24 hours. Allergic reactions toMorinda citrifoliain this study, was not observed. The second study included forty-five Guinea pigs. The study included several what about the test groups using different forms and concentrations of Morinda citrifolia, which corresponded to the negative control group. The test group was induced three times a week for two weeks. After thirty-two days of rest all animals were injected substance and observed the symptoms of allergic response. After the introduction, none of the groups of animals with the introduction of Noni positive allergic reactions were not observed.

13-week study of oral toxicity was performed to further assess the system securityMorinda citrifolia. Eighty rats Spruque Dawley were divided into four groups: a control group and three groups of dosage. Daytime is inserted through the probe dose was 0.4 ml/kg 4 ml/kg and 8 ml/kg of Spent observing animals on adverse clinical signs, food consumption and weight loss. Additionally took blood samples for hematological and clinical chemical analysis at the conclusion of the study. In addition, we measured the weight of individual organs and tissue samples 55 bodies were taken for microscopic examination. All groups showed no handling-related differences in body weight and organs, food consumption, clinical examinations, blood chemistry, hematological measurements and histological examination of tissues.

Spent the second 13-week toxicity study ofMorinda citrifolia. This research who covers higher doses, compared to the previous 13-week study. In this study consisted of three groups of dosage. Evaluate the samples wereMorinda citrifoliaone concentration is 2.5 times more concentratedMorinda citrifoliaand 4 times more concentratedMorinda citrifolia. Concentrated samples were used to achieve a dose equivalent to 50 ml/kg body weight and 80 ml/kg body weight. Protocol and measurements for the second 13-week study were the same as in the first study. The results of this study show that the no-observed-level-adverse-effect (NOAEL) was above 20 ml 4 times more concentratedMorinda citrifolia/kg/day. This is equivalent to 80 ml ofMorinda citrifolia/kg/day. Prospectively, the number is 8% of body weight of the animal. The highest safe level of consumption was still not achieved in these studies. Data show thatMorinda citrifoliacan safely be used in effective amounts.

In addition to those already conducted tests for biocompatibility there are a number of screening tests that can be used to assess the safetyMorinda citrifoliabefore it can be used as a dental material. The screening tests for measuring the biocompatibility of dental materials defined by ISO. ISO represents the t of the international Federation of the main committees of the national standards (the main committees-ISO member bodies). ISO 7405 [international organization for standardization, 2003] cover specific dental materials and ISO 10993 specifically cover medical devices, which also include dental materials. International standard represents the coordination and combination of national standards, including the document number 41 of the American dental Association [Adopted policy directions, 1998; American dental Association], the British standard 5736 [1989] and Deutches Institut for Normung DIN V 13 930 [1996]. ISO defines dental material as a substance or combination of substances, specifically obtained and/or presented for use by the authorized entity in dental practice and/or related procedures. Specified the main goal of ISO 10993, entitled "Biological evaluation of medical devices", is to protect people [international organization for standardization, 2003]. It is the most common nowadays is a guiding document for selection of tests that should be used to assess biological responses related to medical or dental materials and safety devices. ISO 7405 entitled "Preclinical evaluation of biocompatibility of medical devices used in dentistry - methods testireba the Oia dental materials" [international organization for standardization 2003] and relates to preclinical testing of materials, used in dentistry, and additional materials ISO 10993.

Guidance ISO 10993 and 7405 recommended standard practices for biological evaluation of dental materials. Briefly, they include: (i) the manufacturer of dental materials rests with the selection of appropriate tests based on the alleged use of materials and known or suspected toxicity profile of the material or its components. (ii) the Manufacturer may choose one of the three tests for cytotoxicity, preferring one compared to the other for reasons of cost, work experience and other reasons. (iii) New materials should be evaluated using the original cytotoxicity and secondary screening tests tissues before advanced animal testing and clinical trials. (iv) the test Results should always be interpreted in the specified by the manufacturer of the application of the material.

For the implementation of phase II of this research proposal, the authors present invention was planned to evaluateMorinda citrifoliain accordance with the first phase of the program, biological testing, recommended by ISO. If these studies are successful, the authors will further other pre-clinical phase of the program, biological testing in the next study.

Permanent cell line of mouse fibroblasts (clone L-929) has provided a means for a good reproducibility when testing the cytotoxicity between different laboratories. This cell line grows easily and is widely used for biological screening dental materials. Cell line L-929 will continue to grow until confluence in the culture cups 50 ml using a modified method of Dulbecco eagle medium (VWR, Suwanee, GA, USA), 10% fetal bovine serum (VWR, Suwanee, GA, USA) and 1% penicillin and streptomycin (VWR, Suwanee, GA, USA). Cell culture will be maintained in an incubator in an atmosphere of 5% carbon dioxide at 37°during the testing period. Immediately before testing, the cells will stain with neutral red vital dye (VWR, Suwanee, GA, USA). A thin layer of agar will be received and placed on top of the cell cultures using sterile methods. Millitary filter of cellulose acetate is brought into contact with confluent cell cultures. A concentrated solutionMorinda citrifoliawill be diluted in 10-fold concentrations (dose, 1×, 10×, 100× 1000×) and 50 μl is loaded on the filters. For each concentration will be obtained for five replicates. The testing period will be 24 hours. During this period, any p is streamie substances must diffuse through the pores of the filter with a diameter of 0.45 μm to provide any cytotoxic effect on the cells. The appearance of the tested leachate between areasMorinda citrifolia-contact with the cell will be registered in accordance with the scoring system for the classification of cytotoxic response. The presence of the discharge of toxic substances fromMorinda citrifoliawill be manifested by loss of the dye inside the cells, because they will be subjected to lysis. The ability of this simple strategy to determine the cytotoxic danger of material in vitro and to reduce the data to systems in vivo has proven successful as the results are easily interpreted.

1. Composition for the care of the oral cavity, comprising an effective amount of at least one of Morinda citrifolia product in the form selected from the group consisting of fruit of the Morinda citrifolia fruit extract, Morinda citrifolia, concentrate fruit juice of the fruit of Morinda citrifolia, Morinda citrifolia oil, powder of leaves of Morinda citrifolia and extract of the leaves of Morinda citrifolia; and active ingredients selected from the group consisting of binders, tonic medicinal extracts, abrasive or polishing agents, deodorizing and bleaching agents, wound healing and inhibiting inflammation substances and media.

2. The composition according to claim 1, where the carrier comprises ingredients selected from the group consisting of an emulsifier, alcohol, sweeteners, thickeners, surface-active substances is STV, suspendida agents, proofreaders taste, preservatives, flavouring buffers, colorants, dyes, pigments, antimicrobial agents, which impart opacity agents, gelling agents, pastes, pH buffers, and combinations thereof.

3. The composition according to claim 1, where the media further comprises a substance selected from the group consisting of glycerin, food polynuclear alcohols, polyols and combinations thereof.

4. The composition according to claim 3, where the polyols are selected from the group consisting of glycerin, propylene glycol, propylene glycol and glycerin, polyethylene glycol, isomaltitol, xylitol, maldita, sorbitol, mannitol and combinations thereof.

5. The composition according to claim 1, where the carrier includes a flavoring selected from the group of compounds consisting of oil of peppermint, oil of gaultheria supine, oil curly mint, clove oil from flower buds, oil, parsley, eucalyptus oil, menthol, Montana, anethole, methyl salicylate, eucalyptol, Chinese cinnamon oil, 1-methyl acetate, sage, eugenol, Oksanen, alpha irisone, marjoram, lemon, orange, propenolatomethyl, cinnamon, vanilla, thymol, linalool, glycerinate cinnamic aldehyde, N-substituted p-Menten-3-carboxamido, 3,1-methoxypropane-1,2-diol and combinations thereof.

6. The composition according to claim 1, which contains active compounds selected from the group consisting of chlorine dioxide, fluoride, alcohols, triclosan, domainbased, chloride of cetylpyridinium, calcium lactate, salts of calcium lactate, sodium fluoride, fluoride of divalent tin, monophosphate sodium, chloride cetylpyridinium, zinc salts, pyrophosphate, 1-hydroxyethane-1,2-diphosphonic acid, 1-phosphonopropyl-1,2,3-tricarboxylic acid, azacycloheptane-2,2-diphosphonic acids, cyclic aminophosphonic acids and their combinations.

7. The composition according to claim 1, where the composition is represented in the form of a toothpaste, gel, liquid for rinsing the oral cavity, means for rinsing the oral cavity, chewing gum, spray to the oral cavity, lozenges, subgingival fluid for irrigation, dental silk threads in the shell, a bristle for a toothbrush, mirrorimages toothbrush, topically applied solution, all chewy candies, lollipops, Ledentsov tiles and nougat or combinations thereof.

8. The composition according to claim 2, where the sweetener is chosen from the group consisting of saccharin, fructose, xylitol, salt saccharin, thaumatin, aspartame, D-tryptophan, dihydrochalcones, Acesulfame, cyclamate salts and combinations thereof.

9. The composition according to claim 2, where giving opacity agents are selected from the group consisting of calcium citrate, esters of rosin, of an emulsion of vegetable gums, triglycerides of Caprylic/capric acids, gua the OIC gum, Arabian gum and high-stability oils.

10. The composition according to claim 1, where abrasives or polishing agents are selected from the group consisting of chalk, calcium carbonate, dicalcium phosphate, aluminum silicate, calcium pyrophosphate, finely dispersed synthetic resins in the form of particles, silica, alumina, three-hydrate of alumina, hydroxyapatite, finely dispersed insoluble metaphosphate sodium in the form of particles, xerogel silica, hydrogel silicon dioxide, precipitated silicon dioxide, three-hydrate of aluminum oxide and finely dispersed aluminum oxide in the form of particles and their combinations.

11. The composition according to claim 2, where the preservatives and antimicrobial agents are selected from the group consisting of p-hydroxybenzoic acid, methyl complex ether, ethyl ether complex, complex propyl ether; sorbate sodium; sodium benzoate, bremgarten, esters phenylsalicylate acid, thymol, and combinations thereof.

12. The composition according to claim 2, where the pH buffers are selected from the group consisting of primary, secondary or tertiary phosphates of alkali metals, citric acid, sodium citrate and combinations thereof.

13. The composition according to claim 1, where the media contains wound healing and inhibiting inflammation substance selected from the group consisting of allantoin, urea, azulene, the active substances chamomile, derivatives of ACE EliLilly acid and combinations thereof.

14. The composition according to claim 2, where the specified gelling agent selected from the group consisting of polycarboxylic acids, salts of polycarboxylic acids, polysaccharides, derivatives of polysaccharides, proteins, derivatives of proteins, polyalkyleneglycol and colloidal silicon dioxide.

15. The composition according to claim 1, where the specified effective amount of Morinda citrifolia is from about 8 to 33% by weight of the composition, and where the specified media ranging from 75 to 90% by weight of the composition.

16. The composition according to claim 1, where the specified effective amount of Morinda citrifolia is about 5 to 25% by weight of the composition, and where the specified media includes glycerin, calcium carbonate and silicon dioxide.

17. The composition according to item 16, where the specified glycerin is from 10 to 50% by weight of the carrier, the said calcium carbonate is from 20 to 40% by weight of the carrier, and the said silica is from about 20 to 40% by weight of the carrier.

18. Method for the treatment and prevention of disorders of the oral cavity and dental irregularities, including the introduction of an effective amount of a composition according to claim 1.

19. The method according to p, where the aforementioned introduction of the specified composition for the care of mouth cavity further includes a local application of the specified composition for the care of the oral cavity by at least one of the teeth, gums and surrounding tissues of the mouth on the spine of the specified patient.

20. The method according to p, where the aforementioned introduction of the specified composition for the care of mouth cavity further includes oral administration of the indicated patient a specified composition for the care of the oral cavity as a food additive.

21. The method according to p, where the carrier includes ingredients selected from the group consisting of an emulsifier, alcohol, sweeteners, thickeners, surface-active substances, suspendida agents, proofreaders taste, preservatives, flavouring buffers, coloring agents, dyes, pigments, antimicrobial agents, which impart opacity agents, gelling agents, pastes, pH buffers, glycerin, food polynuclear alcohols, polyols and combinations thereof.

22. The method according to item 21, where the polyols are selected from the group consisting of glycerin, propylene glycol, propylene glycol and glycerin, polyethylene glycol, isomaltitol, xylitol, maldita, sorbitol, mannitol and combinations thereof.

23. The method according to p, where the media contains flavorings selected from the group consisting of oil of peppermint, oil of gaultheria supine, oil curly mint, clove oil from flower buds, oil, parsley, eucalyptus oil, menthol, Montana, anethole, methyl salicylate, eucalyptol, Chinese cinnamon oil, 1-methyl acetate, sage, eugenol, Oksanen, alpha irisone, marjoram is, lemon, orange, propenolatomethyl, cinnamon, vanilla, thymol, linalool, glycerinate cinnamic aldehyde, N-substituted p-Menten-3-carboxamido, 3,1-methoxypropane-1,2-diol and combinations thereof.

24. The method according to p, where the composition comprises an active compound, selected from the group consisting of chlorine dioxide, fluoride, alcohols, triclosan, domainbased, chloride of cetylpyridinium, calcium lactate, salts of calcium lactate, sodium fluoride, fluoride of divalent tin, monophosphate sodium, chloride cetylpyridinium, zinc salts, pyrophosphate, 1-hydroxyethane-1,2-diphosphonic acid, 1-phosphonopropyl-1,2,3-tricarboxylic acid, azacycloheptane-2,2-diphosphonic acids, cyclic aminophosphonic acids and their combinations.

25. The method according to p, where the composition is represented in the form of a toothpaste, gel, liquid for rinsing the oral cavity, means for rinsing the oral cavity, chewing gum, spray to the oral cavity, lozenges, subgingival fluid for irrigation, dental silk threads in the shell, a bristle for a toothbrush, mirrorimages toothbrush, topically applied solution, all chewy candies, lollipops, Ledentsov tiles and nougat or combinations thereof.

26. The method according to item 21, where the sweetener is chosen from the group consisting of saccharin, fructose, xylitol, salt saccharin, thaumatin, aspartame, D-tryptophan, Digi is regalano, Acesulfame, cyclamate salts and combinations thereof.

27. The method according to item 21, where giving opacity agents are selected from the group consisting of calcium citrate, esters of rosin, of an emulsion of vegetable gums, triglycerides of Caprylic/capric acid, guar gum, Arabia gum, and high-stability oils.

28. The method according to p where abrasives or polishing agents are selected from the group consisting of chalk, calcium carbonate, dicalcium phosphate, aluminum silicate, calcium pyrophosphate, finely dispersed synthetic resins in the form of particles, silica, alumina, three-hydrate of alumina, hydroxyapatite, finely dispersed insoluble metaphosphate sodium in the form of particles, xerogel silica, hydrogel silicon dioxide, precipitated silicon dioxide, three-hydrate of aluminum oxide and finely dispersed aluminum oxide in the form of particles and their combinations.

29. The method according to item 21, where the preservatives and antimicrobial agents are selected from the group consisting of p-hydroxybenzoic acid, methyl complex ether, ethyl ether complex, complex propyl ether; sorbate sodium; sodium benzoate, bremgarten, esters phenylsalicylate acid, thymol, and combinations thereof.

30. The method according to item 21, where the pH buffers are selected from the group consisting of primary, secondary or tertiary phosphates, alkali m is the metal, citric acid, sodium citrate and combinations thereof.

31. The method according to p, where wound healing and inhibiting inflammatory substances selected from the group consisting of allantoin, urea, azulene, the active substances chamomile, derivatives of acetylsalicylic acid, and their combinations.

32. The method according to item 21, where the specified gelling agent selected from the group consisting of polycarboxylic acids, salts of polycarboxylic acids, polysaccharides, derivatives of polysaccharides, proteins, derivatives of proteins, polyalkyleneglycol and colloidal silicon dioxide.

33. The method according to p, where the specified effective amount of Morinda citrifolia is from about 8 to 33% by weight of the composition, and where the specified media ranging from 75 to 90% by weight of the composition.

34. The method according to p, where the specified effective amount of Morinda citrifolia is about 10 to 50% by weight of the composition, and where the specified media is from about 48 to 88% by weight of the composition.

35. The method according to p, where the specified effective amount of Morinda citrifolia is about 5 to 25% by weight of the composition, and where the specified media includes glycerin, calcium carbonate and silicon dioxide.

36. The method according to p where specified glycerin is from 10 to 50% by weight of the carrier, the said calcium carbonate is from 20 to 40% by weight of the carrier and the silicon dioxide comp is made from about 20 to 40% by weight of the carrier.



 

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FIELD: personal hygiene commodities.

SUBSTANCE: invention relates to aqueous fluid compositions and aims at obtaining fluid non-Newton on shear liquefiable composition suitable for use in personal hygiene commodities, which composition is capable of slurring water-insoluble particles and manifests stability under freezing/thawing conditions. Invention provides fluid composition containing, based on the mass of composition, at least one anionic surfactant, 5-30%, at least one alkanolamine, 0.1-10%, at least one electrolyte, and water. Composition may further contain one or several supplemental surfactants. Composition of invention has layered liquid-crystalline structure, has non-Newton on shear liquefaction property, and ability of slurring water-insoluble particles or partially insoluble components, while being stable in at least one freezing/thawing cycle. Proposed are also a method of preparing indicated composition or stable multiphase composition based thereon.

EFFECT: extended choice of practically useful on shear-liquefiable compositions.

54 cl, 11 tbl, 5 ex

FIELD: personal hygiene commodities.

SUBSTANCE: invention relates to aqueous fluid compositions and aims at obtaining fluid non-Newton on shear liquefiable composition suitable for use in personal hygiene commodities, which composition is capable of slurring water-insoluble particles and manifests stability under freezing/thawing conditions. Invention provides fluid composition containing, based on the mass of composition, at least one anionic surfactant, 5-30%, at least one alkanolamine, 0.1-10%, at least one electrolyte, and water. Composition may further contain one or several supplemental surfactants. Composition of invention has layered liquid-crystalline structure, has non-Newton on shear liquefaction property, and ability of slurring water-insoluble particles or partially insoluble components, while being stable in at least one freezing/thawing cycle. Proposed are also a method of preparing indicated composition or stable multiphase composition based thereon.

EFFECT: extended choice of practically useful on shear-liquefiable compositions.

54 cl, 11 tbl, 5 ex

FIELD: medicine; cosmetology.

SUBSTANCE: invention relates to the agents for elimination of grey hair. The agent contains the chromogenic agent, which contains the beer yeast, hop and water in the following correlation, in mass%: 5-10 of the beer yeast, 2.5-5 of hop, water - the rest.

EFFECT: these components are harmless and provide efficient restoration of dark color in grey hair.

1 tbl, 3 ex

FIELD: medicine; cosmetology.

SUBSTANCE: invention relates to the agents for elimination of grey hair. The agent contains the chromogenic agent, which contains the beer yeast, hop and water in the following correlation, in mass%: 5-10 of the beer yeast, 2.5-5 of hop, water - the rest.

EFFECT: these components are harmless and provide efficient restoration of dark color in grey hair.

1 tbl, 3 ex

FIELD: medicine; cosmetology.

SUBSTANCE: invention relates to the cosmetic agents, namely cosmetic agent for care and maintenance of the natural hair function; contains the routine cosmetic raw materials, differs in containing the 2-furanon derivative , at least one polymeric compound and at least one compound of protein hydrolysates and their derivatives.

EFFECT: invention efficiently maintains natural hair function.

6 cl, 32 ex, 1 dwg

FIELD: medicine; cosmetology.

SUBSTANCE: invention relates to the cosmetic agents, namely cosmetic agent for care and maintenance of the natural hair function; contains the routine cosmetic raw materials, differs in containing the 2-furanon derivative , at least one polymeric compound and at least one compound of protein hydrolysates and their derivatives.

EFFECT: invention efficiently maintains natural hair function.

6 cl, 32 ex, 1 dwg

FIELD: medicine; cosmetology.

SUBSTANCE: invention relates to the cosmetic agents, namely cosmetic agent for care and maintenance of the natural hair function; contains the routine cosmetic raw materials, differs in containing the 2-furanon derivative , at least one polymeric compound and at least one compound of protein hydrolysates and their derivatives.

EFFECT: invention efficiently maintains natural hair function.

6 cl, 32 ex, 1 dwg

FIELD: medicine; cosmetology.

SUBSTANCE: composition for local application contains: i) anti-dandruff agent; ii) conjugated linoleic acid and iii) cosmetic acceptable dissolvent or carrier.

EFFECT: invention provides increased efficiency in treatment and prevention of dandruff and itching of scalp skin.

14 cl, 1 dwg, 2 ex

FIELD: medicine; cosmetology.

SUBSTANCE: composition for local application contains: i) anti-dandruff agent; ii) conjugated linoleic acid and iii) cosmetic acceptable dissolvent or carrier.

EFFECT: invention provides increased efficiency in treatment and prevention of dandruff and itching of scalp skin.

14 cl, 1 dwg, 2 ex

FIELD: medicine.

SUBSTANCE: concentrated solution of stabilized ozone for treatment of inflammatory processes contains the ozone carrier, which includes the isotonic saline and ozone, and in addition, perfluorocarbon or mixture of perfluorocarbons and the surfactant. The components are taken in particular amount.

EFFECT: concentrated solution of stabilized ozone has long-lasting antimicrobial activity and storage time.

6 ex, 2 dwg

FIELD: medicine.

SUBSTANCE: bioactive food additive contains the products of Morinda citrifolia processing, which is used to inhibit the aromatase or aromatase enzymes transforming the androgens to estrogens, to inhibit the receptor binding to estrogens and to decrease and/or regulate the estrogen production. The invention reduces and regulates the estrogen production.

EFFECT: efficient in treatment of estrogen-dependent cancer and conversion of estrogen-dependent malignant tumors.

60 cl, 1 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: strain of Tramertes pubescens VKPM Р-839 is characterised by increased ergosterine synthesis. The ergosterine content in biomass is 0.25 g/100 g. The medicine is made of dried and purified biomass of strain of Tramertes pubescens VKPM Р-839, which contains total protein 45 weight%, carbohydrates 27 weight%, lipids 4.5 weight%, nucleic acids 4 weight%, mineral substances 7 weight%), vitamins 0.4 weight%,).

EFFECT: medicine stimulates the immunogenesis and promotes the liver functional recovery.

2 cl, 12 tbl, 4 ex

FIELD: medicine, oncology, amino acids.

SUBSTANCE: invention relates, in particular, to the development of an antitumor preparation based on natural substances. Invention relates to an amino acid preparation comprising at least one modified essential amino acid obtained by treatment of amino acid by ultraviolet radiation (UV) at wavelength 250-350 nm for 12-80 h at temperature 15-30oC or with ozone at temperature 15-25oC. The modified amino acid has no toxicity for health cells. Also, invention relates to a method for preparing such preparation. Invention provides the development of an antitumor preparation based on modified amino acids and expanded assortment of antitumor preparations being without cytotoxicity for normal cells.

EFFECT: valuable medicinal antitumor properties of preparation.

8 cl, 4 tbl, 2 dwg, 4 ex

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