Strain of tramertes pubescens c-23 fungus - ergosterine producer and agent, which positively affects tissue metabolism, stimulates immunogenesis and promotes liver oxydase-mixed functional recovery

FIELD: medicine.

SUBSTANCE: strain of Tramertes pubescens VKPM Р-839 is characterised by increased ergosterine synthesis. The ergosterine content in biomass is 0.25 g/100 g. The medicine is made of dried and purified biomass of strain of Tramertes pubescens VKPM Р-839, which contains total protein 45 weight%, carbohydrates 27 weight%, lipids 4.5 weight%, nucleic acids 4 weight%, mineral substances 7 weight%), vitamins 0.4 weight%,).

EFFECT: medicine stimulates the immunogenesis and promotes the liver functional recovery.

2 cl, 12 tbl, 4 ex

 

The technical field

The present invention relates to new strains of microorganisms and drugs produced on the basis of these strains.

The most effective present invention can be used in cancer and liver disease.

Prior art

It is known that fruit bodies of fungi of the genus Trametes (modern synonym of the previously used name of the genus Coriolus), in particular, C.versicolor used in folk medicine in Japan as a medicinal plant (Cochran K.W. The biololigy and cultivation of edible mushroom. - New York: Acad. Press., 1978, p.168-197; Ikekawa et al., 1968). Hypolipidemic activity of these fungi is also confirmed in animal experiments (Cohran, 1978; Ginter, Bobek, 1988).

With hypolipidemic and hypocholesterolemic activity is closely linked to their anti-atherogenic and anti-sclerotic effect. According to the Institute of nutrition alcoholic extract of the fruiting bodies of Trametes pubescens has in vitro anti-sclerotic activity, which is manifested in the reduction of intracellular total cholesterol and inhibition of cell proliferation (Lee Hwa Ren and others, 1989; Ryong et al., 1989).

It is also known for the treatment and prevention of hyperlipidemia (In Jp Application No. 3-61651, AK 35/84, publ. 91.09.20 No. 3-1542)containing glycoprotein obtained by the extraction of fruit bodies or cultured mycelium of Trametes.

Long ago Paul the sugars from mushroom Trametes used as therapeutic agents for pneumonia, bronchitis and other diseases (U.S. patent No. 4289688, 1979, the priority of Japan No. 40105, 1979). There is evidence that nitrogen-containing polysaccharides (glycoproteins) from T.versicolor increase the sensitivity of bacteria to antibiotics (France application No. 2387038, 1978). In particular, the patented product obtained by extraction of the mycelium of the fungus Trametes, which has Antirheumatic, hemostatic, analgesic, hypolipidemic and anti-inflammatory action. The drug lowers blood sugar, increases the blood flow in the coronary vessels, lowers blood pressure, improves the protein content in the blood, improves proteinemia, regulates the formation or metabolism of prostaglandins (the Federal Republic of Germany patent No. 3429551, 1985).

Polysaccharides extracted from T.versicolor, possess antiviral and immunostimulatory activity (Chen et al., 1986). In particular, from the mycelium and fruiting bodies of Trametes by extracting offered to get a drug for the treatment of retroviral infections, such as AIDS (EPO application No. 0295962, 1989), and nitrogen-containing polysaccharide from Trametes restored the level of the immune response in the body in case of loss (U.S. patent No. 469978, 1987).

Protein-polysaccharide complexes with antitumor activity, extracted from the mycelium and fruit bodies of such species of the genus Trametes, as T.versicolor, T.conchifer (Schw.) Pat., T.pargamenus (fr. Pat., T.hirsutus (Fr.) Quel, T.mioformis (Klotz.) Pat., T.consors (Berk.) Jmaz., T.pubescens (Fr.) Quel. (U.S. patent No. 4209688, 1988; UK patent No. 1 521168, 1978; France application No. 2335235, 1977; Japan patent No. 150114, 1975; UK patent No. 1538399, 1979).

The most well-known drug, derived from the fungus Trametes versicolor, is the christening (PSK). In Japan christening issued since 1977 the company "Sankyo" (Chemical-pharmaceutical production abroad. Express information. UNISANTE, 1988, issue 1, p.7-12). It is known that the christening affects T-cell-mediated immunity (VA, Denian et al, 1981). Found that it enhances the body's resistance to certain infectious diseases, probably due to activation of macrophages (Denin et al 1981.) In clinical practice immunological properties Kristina used in the treatment of cancer (Akiyoshi, Tsuyoshi on et al., 1981).

Known preparations containing related proteins polysaccharides extracted from the fruiting bodies of Trametes pubescens with antitumor activity (U.S. patent No. 4289688, 1981; Japan patent No. 704431, 1979), and nitrogen-containing polysaccharides (UK patent No. 1521168, 1978; France application No. 2335235, 1977, the priority of Japan No. 150114, 1975; Japan's bid (In) No. 63-28413, 1988). However, therapeutic efficacy of these drugs is low. Synthesis of ergosterol known strains of the fungus and the availability of it products based on biomass or fruit bodies Trametespubescens, not found.

The closest analogue (prototype) of the declared object is a method of producing polysaccharide with increased antitumor and immunostimulating effect by extraction Trametes pubescens water solvent with a pH of 4-10 at a temperature 3-27°C, branch substances with a molecular weight of not less than 8000 and processing of the extract by the gel filtration method when 3-27°With (Japan, application No. 63-28418 declared 57-57-122016 82 07 15, publ. 88.06.08 No. 3-711, the applicant Fujiwara Muzizi etc. AND C 35/84, SW 37/00).

However, the tool obtained by a specified method, also not efficient enough, and all attempts to improve it have failed.

Apparently, to achieve the required efficiency using the above means, even by their improvement is impossible, so the present invention is to nd the most promising strain of the fungus Trametes pubescens, and the creation of more effective drugs that have hepatoprotective, annotationname and immunomodulatory properties that meet modern medicine.

Disclosure of inventions

The problem is solved by the fact that found a new strain that is registered as a strain-23 (VKPM F-839) of the fungus Trametes pubescens, possessing more effective than the well-known strains, hepatoprotective, annotationname immunomoduliruushimi properties a method of deep cultivation of the biomass of this strain, which gives the possibility to obtain it on an industrial scale, and on the basis of this strain created absolutely safe for human and animal drug, significantly stimulating the immunogenesis of the body and contributing to the restoration of disturbed oxidase-mixed liver function. The drug is easily made and stored, since it consists only of dried and separated from impurities biomass natural strain-23 VKPM F-839 fungus Trametes pubescens. The strain deposited at the all-Union collection of industrial microorganisms "Vniigenetika" under registration number VKPM F-839.

The biomass of a new strain C-23 (VKPM F-839) of the fungus Trametes pubescens get bioengineering by submerged culture in a sterile liquid nutrient medium containing carbohydrates, mineral nitrogen - and phosphorus-containing salts and urea.

The culture is grown in a fermentation apparatus for aeration of 0.1 to 2.0 rpm rpm with a stirring 50-300 rpm or without, at a temperature of 20-40°With, in the range of pH 3.0-7.5 on a medium containing molasses, ammonium compounds, phosphates and urea. The cultivation process can be 24-72 hours.

Seeds contribute in a wide range - from 2% to 50% of the medium volume. It is prepared as follows. Culture grown on garitano wort in test tubes, flasks or biological mattresses, transferred into a flask with liquid nutrient medium (e.g., beer, wort, medium with molasses). Insulinopenia can be done agar blocks, crushed pieces of the environment with mycelium, gomogenizirovannykh mycelium grown surface on the liquid medium, or large parts of the colonies without grinding. Inoculated flasks are placed on a rocking chair. The cultivation process is 150-220 hours. Thus obtained deep culture is used as a seed (with gomogenizirovannom or without him) further transfers during laboratory preparation of inoculum for the production. In the flowsheet valid serial cultivation of several generations of culture in a liquid nutrient medium in flasks and apparatus-inoculators before the transfer to the fermentation apparatus.

In the process of growing producer at all stages of the use of microscopic and microbiological purity and morpho-physiological state of the culture. The process of growing monocultures producer is carried out in a strictly sterile conditions. Used to prepare the environment molasses pre-screened for biological purity.

The cultivation of the producer may be periodic or supply-detachable p is the bench.

After completion of the cultivation process the biomass is separated by filtration, washed with cold water, pressed and dried with hot air at a temperature of at least 70°C, then subjected to grinding and standardization. The finished drug mark, pasportizirovat and hermetically Packed.

Characteristics of culture

Cultural and morphological characteristics.

On wort agar culture of the fungus forms colonies initially white fluffy khlopkovaia-woolly, then felt, eventually forming a leathery membrane. The peripheral part of the colony is less dense. The edge of the colony is smooth, adpressed, reversum not changed, then whitened. In aging colonies become yellowish or white shade, form the beginnings of the fruiting bodies of cream color in the form of tubercles 3-10 mm in diameter, often confluent, or fruiting bodies in the form of sit-hats-ROM with raised edges, often confluent, with unclear zoning, white, yellowing over time to cremello color. Sometimes formed fruiting bodies (always single layer) with well developed hymenophore and basidiospores (light yellow powder). The smell is pleasant, mushroom. The Petri dish with a diameter of 90 mm at a temperature of 26°With overgrown with fungal culture for 6 days.

Hyphae septate, septa dolipore. Hyphae of three types (trimi the practical type of mycelium) branched, branching at right angles, the diameter of the hyphae of 0.8 to 1.6 μm; 2.0 to 3.0 μm and 2.0 to 4.0 μm. Forms in culture buckle single, large, steeply, often without clearance. Irregular forms chlamydospores, arthrospira and oidia. It forms prismatic crystals, cubic and a needle shape.

Gives a positive reaction for the presence of extracellular oxidase on Bavendamm. On agar wort with Gallic acid forms a dark pigment, while the diameter of the pigmented area is larger than the diameter of the colony (the colony with a dark brown halo).

On the surface of the liquid wort (4° Balling) forms a fluffy felt the film over time compactible to leathery on the surface of which laid the beginnings of a fruit phone

In the deep culture of filamentous aggregates of spherical or irregular shape. In the Central part of the globules hyphae thickened, curved, large globules on the slice is observed zoning, of the cavity. Depending on the cultivation conditions globule smooth or pubescent, with a diameter of from 1 to 8 mm (usually 2-5 mm). On media with molasses and ethanol are easily transferred to the dispersed form of growth.

Deep culture hyphae actively branching, long, cytoplasm is homogeneous structure, lipid inclusions and vacuoles little, the ends of hyphae rounded. Under unfavourable conditions in the culture of ablauts swollen wide cells of irregular shape. The ends of the hyphae become swollen or sawtoothed shape. Dominated by late age phase, characterised by a large number of lipid inclusions and vacuoles, sometimes inlaid with crystals and drops of oil. The crystals are cubic, prismatic or acicular shape. In dispersed culture is not observed the formation of cubic crystals, abundantly present on the surface of the globules in the old culture. Forms chlamydospores, arthrospira and oidia. Hyphae are branched at right angles, mainly of two types: thin (0,8-1,6 µm) and thick (2,0-4,0 µm). Buckle single large pipe, often without a gap.

Physiological and biochemical characteristics

Culture utilizes glucose, sucrose, mannitol, sorbitol, starch, raffinose, glycerol, galactose, maltose, lactose, arabinose, ethanol.

As the nitrogen source uses organic forms of nitrogen (peptone, urea, asparagine and ammonium salt.

Liquefies gelatin. Milk rolls and peptonized.

Grows in the temperature range from +24°C to +34°withstands temperatures from +2°C to 40°C, optimum temperature radial growth +30°C.

Increases in the range of pH from 3.0 to 7.5 with an optimum of 4.0 to 6.0.

Growth requires oxygen.

Pathogenicity

Strain absolutely not pathogenic and harmless to humans and animals. About what is worn to the ecological group of wood-destroying saprotrophs.

Antagonistic properties

Antibiotics are not formed. Resistant to streptomycin and penicillin, is suppressed by nystatin.

Biotech feature.

When grown in sterile conditions periodically submerged culture medium of the following composition (%): sugar beet molasses - 1.5 for reducing substances; ammonium nitrate - 0,3; potassium phosphate one-deputizing - 0,12; urea - 0,07; with a dose of planting to 10.0 vol.%, in continuous mild stirring and aeration, the corresponding sulfite indicator 0.5 mmol oxygen/l.min, biomass accumulation occurs in 24-48 hours of cultivation and is 7-8 g/l environment on absolutely dry substance.

Storing strain

Strain well preserved on agar wort at a temperature of +4°-+6°with periodic re-seeding after six months.

Preparation on the basis of a new strain C-23 (VKPM F-839) of the fungus Trametes pubescens.

For a better understanding of the invention, most of the parameters of the proposed product are presented in tabular form.

List of tables.

Table 1. The amino acid composition of the drug.

Table 2. Fatty acid composition of the preparation (% of total).

Table 3. The content of biologically active phospholipids.

Table 4. The content of vitamins of group of Century

Table 5. The content of macro - and microelement the century

Table 6. Changes in the level of tumor markers after administration of the proposed new drug.

Table 7. The amino acid composition of preparation example 1.

Table 8. The amino acid composition of preparation example 3.

Table 9. The amino acid composition of preparation example 4.

Table 10. The content of macro - and microelements in preparation example 4.

Table 11. Change indicators reflecting the state of cellular immunity while taking the drug.

Table 12. Change oxidase-mixed liver of the person under the influence of the drug.

Based on the culture of Trametes pubescens strain-23 created the drug, possess hepatoprotective, annotationname, immunomodulatory properties, consisting of dried and separated from impurities biomass natural strain affecting tissue metabolism, stimulating the immunogenesis and contributing to the restoration of disturbed oxidase-mixed liver function. The chemical composition of the drug, wt.%:

Total protein - 40-50;

Carbohydrates - 25-30, including polysaccharides (beta-glikana) - 1-4;

Lipids - 3,5-5,0, including unsaturated fatty acids, phospholipids, ergosterol.

Nucleic acid 3,0-4,5.

Minerals - 6,5-8,5, including calcium, potassium, phosphorus, magnesium, iron, copper, zinc, chromium, cobalt, manganese, molybdenum.

Vitamins: 0,35-0,45, including B1 (thiamine), B2(Riboflavin),3(Pantothenic acid),6(pyridoxine), folic acid, PP (nicotinic acid), Biotin, choline. Water - the rest.

Table 1

The amino acid composition of the drug
Amino acidContent, % ASV
Isoleucine2,7-3,2
Leucine2,6-3,4
Lysine2,7-3,9
Methionine+cystine(0,3-0,5)+(0,9-1,2)
Phenylalanine+tyrosine(1,1-1,6)+(1,2-1,6)
Tryptophan0,80-0,91
Threonine2,0-2,3
Valine1,8-2,1
Histidine1,6-1,8
Arginine2,0-2,2
Aspartic acid3,7-4,2
Series2,2-2,8
Glutamic acid6,0-7,2
Proline1,0-1,4
Glycine2,0-2,7
Alanine2,2-2,9

The overall composition of carbohydrates typical of basidiomycetes. Biologically active fraction of water-soluble polysaccharides beta-glycans in a final product ranges from 1.0 to 4.0%.

In the lipid fraction content of fatty acids is 74.6% of the number of non-polar lipids. Table 2 fatty acid represented only compounds with an even number of carbon atoms, the ratio of them in the faction close to the best vegetable oils.

Table 2

Fatty acid composition of the drug, % from amount
Fatty acidContent, wt. % from amount
Palmitic C16:034,5Total saturated acids - 38,6
Stearic18:04,1
Palmitoleic C16:11,3All essential unsaturated fatty acids - 61,4
Oleic18:1the 33.4
Linoleic C18:225,7
Linolenic18:3About 1.0

Table 3

The content of biologically active phospholipids
The phospholipidContent, wt.% from amount
Phosphatidylethanolamine44
Phosphatidylcholine 49
Phosphatidylserin7

In the composition of the lipid fraction contains a significant amount of ergosterol (substance D-vitamin activity) of 0.20-0.28 g/100 g of the product, as well as the 22-digidrosteron and 5-digidrosteron.

Table 4

The content of b vitamins
VitaminContent, mg/kg dia
B1(thiamine)3,6
In2(Riboflavin)35,8
In3(Pantothenic acid)14,5
In6(pyridoxine)4,2
In12(cobalamine)Traces
Folic acid8,0
PP (nicotinic acid)230
Biotin1-2
Choline3100-4200

The product is rich in calcium, potassium, phosphorus, magnesium, iron and other physiologically active macro - and microelements in organically bound form.

Table 5

The content of macro - and microelements
Content, mg/kgContent, mg/kg
Calcium67000-74000Manganese15-19
Phosphorus6800-8000Molybdenum15-20
Potassium4000-4500Nickelthe 3.7 to 7.9
Magnesium700-800Chromeof 5.0 to 10.5
Iron300-400Cobalt0,1-1,6
Copper31-44Fluoride2,9
Zinc90-200

The harmlessness of the drug is confirmed by the results of biomedical check performed by the Institute of nutrition. It is shown that the drug does not cause allergic reactions and has no contraindications for use. At the conclusion of the Institute, the drug received approval of the Ministry of health and the state Committee of sanitary.

Therapeutic and prophylactic properties of the proposed drug was studied by determining the number of tumor markers present in the blood of cancer patients before and after the course of treatment with the proposed medication was assessed blood levels of the following tumor markers: cancer-embryonic antigen (CEA), ferritin, carbohydrate antigen (CA-125) and muzykopodobnyh antigen (MTSA).

Determination of the levels of tumor markers in serum was carried out radioimmunoassay and enzyme immunoassay methods. For studies used a test system of foreign and domestic firms. Radiometry serum was held gamma counter 1175 firm "Searle". The data are processed statistically by the method of Maceviciute-öhringen E.N.

The analyses carried out on completion of the course receive a new proposed drug, showed a significant reduction of the levels of all defined tumor-associated antigens (table 6).

Table 6

Changes in the level of tumor markers in the serum after a course of reception of a new proposed drug
MainControlHealthy
Indicatorsthe group of patientsthe group of patientsface
x)XX)
Number
surveyed, people413810
Ferritin, μg/l:
before the treatment152,6±19,0142,4±11,961,4±5,6
after the treatment91,7±22,6126,1±8,6
REA, μg/l:
before the treatment14,1±2,411,8±2,44,1±0,3
after the treatment7,6±1,29,4±2,1
CA-125 u/l:
before the treatment11,4±2,312,1±2,65,9±0,1
after the treatment5,2±0,411,3±2,1
MTSA, u/l:
before the treatment9,3±1,69,1±1,86,8±0,8
after the treatment6,1±1,98,4±1,6
x) for patients treated with the use of new medication
XX) patients treated by traditional methods.

The possibility of obtaining a drug indicated earlier, the level of industrial way and use to obtain a culture of the fungus Trametes pubescens strain-23 (VKPM F-839) as producer is illustrated by the following examples.

Example 1.

The culture of the strain was stored in tubes at oblique agar wort at a temperature of +(4-6)°C.

Sowing liquid nutrient medium initially carried out by transferring the culture together with a piece of agar medium (first passage), and then with a liquid medium were transferred to liquid at the rate of 10-20 vol.% inoculum (second, third, and subsequent passages).

Growing inoculum was performed in a temperature-controlled room at a temperature of 28°and the speed of the swing 180 rpm on a medium of the following composition, wt.%: molasses beet - 3,0; ammonium nitrate - 0,3; potassium phosphate one-deputizing - 0,12; urea - 0,07; antifoam propanol - 0,05; tap water - the rest; pH 5.5. Nutrient medium was sterilized at a temperature of 138°C, the holding time is 5 minutes

The duration of the growing seed: the first passage 144 hours, the second passage 96 hours, the third and subsequent 48 hours.

Deep culture used for inoculation of inoculator capacity of 250 liters with 150 l of sterile nutrient medium of the above composition. The quantity of inoculum was 5 L. the Cultivation was carried out for 48 hours at a temperature of 28°With mechanical agitation intensity 180 rpm and aeration of 150 l/min

Grown seed before the Ali for the planting of the main production fermenter with a capacity of 10 m 3with 7 m3sterile nutrient medium. The process of growing the culture in the fermenter was carried out at 28°without mechanical agitation with aeration 7 m3/min for 40 hours. At the end of the fermentation culture liquid was applied to a filter press for separation of biomass, washing it with water and carefully blowing compressed air to produce a dense layers of biomass. Biomass was dried in a fluidized bed dryer at a temperature of 70°C. the Obtained dry biomass was ground and was standardized. The amount of the finished product - 49 kg

The resulting preparation had the following composition, wt. %: total protein - 49,4, nucleic acid - 4,5, lipids 5,0, minerals and 6.5, including, mg/kg zinc - 197,9; manganese - 15,5; copper - 42,4; cobalt is 0.55. The content of beta-glycans in a preparation of 1.55%. The content of ergosterol - 0.20 g/100 g product. The amino acid composition of the drug are shown in table 7.

Table 7

The amino acid composition of preparation example 1
Amino acidContent, % ASV
Isoleucine2,9
Leucine2,8
Lysine3,2
Methionine+cystine0,5+0,9
Phenylalanine+tires is n 1,6+1,2
Tryptophan0,84
Threonine2,3
Valine2,1
Histidine1,7
Arginine2,2
Aspartic acida 3.9
Series2,6
Glutamic acid6,6
Proline1,3
Glycine2,2
Alanine2,8

After analysis of the quality of the preparation was subjected to packaging.

Example 2

Growing seed in flasks and cultivated the apparatus was carried out similar to that presented in example 1.

Grown seed passed to planting the main production fermenter with a capacity of 10 m3with 7m3sterile nutrient medium of the following composition, wt.%: molasses beet - 4,0; ammonium nitrate - 0,3; potassium phosphate one-deputizing - 0.12; urea - 0,07; tap water - the rest. The fermentation process was carried out at 28°without mechanical agitation with aeration 7 m3/min for 48 hours. Sterile molasses was applied to the apparatus by fractional aid.

Processing the culture fluid and getting the finished product was carried out by the Academy of Sciences of the logical set forth in example 1. The amount of the finished product - 91 kg

The total protein content in the resulting product is 50%of the lipids of 3.5%, nucleic acid - 4,5%, carbs 30%, including beta-glycans of 2.6%. The content of ergosterol - 0.25 mg/100 g product.

Example 3

Growing seed in flasks and cultivated the apparatus was carried out similar to that presented in example 1.

Grown seed passed to planting the main production fermenter with a capacity of 10 m3with 7 m3sterile nutrient medium. The fermentation process was carried out at 28°With stirring under aeration 7 m3/min for 40 hours. Then 6.3 m3the culture fluid was transferred to filter and filled up the apparatus the same amount of fresh sterile nutrient medium. Fermentation is continued in the above mode for 20 hours. At the end of the second and subsequent cycles of fermentation culture liquid was processed as described in example 1. The amount of the finished product - 44 kg in the first cycle and 50 kg in the second and subsequent cycles.

The resulting product has the following composition, wt.%: total protein - 45,0; nucleic acid - 3,5; lipids - 3,8; beta-glikana - 4,0; minerals - 8,0, including, mg/kg zinc - 200,0; manganese - 16,4; copper - 40,3; cobalt - 0,62. The amino acid composition of the product p is eveden in table 8.

Table 8

The amino acid composition of preparation example 3
Amino acidContent, % ASV
Isoleucine2,9
Leucine2,9
Lysine2,9
Methionine+cystine0,3+0,9
Phenylalanine+tyrosine1,3+1,4
Tryptophan0,84
Threonine2,2
Valine1,9
Histidine1,8
Arginine2,1
Aspartic acidthe 3.8
Series2,4
Glutamic acid6,5
Proline1,3
Glycine2,2
Alanine2,4

Example 4

Growing seed in flasks and cultivated the apparatus was carried out similar to that presented in example 1.

Grown seed passed to planting the main production fermenter with a capacity of 10 m3with 7 m3sterile nutrient medium of the following composition, wt. %: raw sugar - 2,0 (reducing substances); ammonium attackis the th - 0,3; potassium phosphate one-deputizing - 0,12; urea - 0,07; corn extract evaporated to 0.5; water water - the rest. The fermentation process was carried out at 28°without mechanical mixing, aeration 7 m3/min for 48 hours.

Processing the culture fluid and getting the finished product was carried out similar to that presented in example 1. The amount of the finished product - 62 kg

The resulting product has the following composition, wt. %: total protein - 40,0; nucleic acid - 3,0; carbohydrates - 25, including beta-glikana - 1,0%; lipids - 4,4; minerals is 8.5. The content of ergosterol - 0.28 mg/100 g product.

Amino acid and mineral composition of the drug are shown in tables 9 and 10.

Table 9

The amino acid composition of preparation example 4
Amino acidContent, % ASV
Isoleucine2,9
Leucine2,7
Lysinea 3.9
Methionine+cystine0,3+1,2
Phenylalanine+tyrosine1,3+1,4
Tryptophan0,9
Threonine2,3
Valine2,0
Histidine1,
Arginine2,2
Aspartic acid4,2
Series2,6
Glutamic acid7,2
Proline1,3
Glycine2,5
Alanine2,9

Table 10

The content of macro - and microelements in the preparation according to example 4
Content, mg/kgContent, mg/kg
Calcium70500Manganese18
Phosphorus8000Molybdenum20
Potassium4500Nickel7,9
Magnesium750Chrome10,0
Iron360Cobalt1,42
Copper42Fluoride2,9
Zinc105

The advantage of the proposed drug before the drug is the prototype, namely its broader biological activity and safety, see "About Islena utility".

Industrial utility

As already mentioned, one of the main features of the drug is its positive effect on the immune system.

In the light of modern concepts of immunological reactivity is considered as a universal protective reaction, serving as an indicator of the overall resilience and resistance of the organism.

Immunological homeostasis is functional substrate, which are lymphocytes: thymusdependent (T-lymphocytes), templatevalue (b cells) and macrophages (cells). T-system of immunity implements the immune response of the cell type, the system implements the humoral response, while the T-system controls operation of In-system.

The decrease in the protective activity of the organism contributes to various diseases, including infectious, and hypernatremia immunity due to sensitization of the organism, leading to the development of allergic and autoimmune diseases. In this regard, relevant research products and products from vegetable raw materials with immunomodulatory properties.

From the literature and the results of their research it is known that polysaccharides of fungal origin have a stimulating effect on the immunogenesis. Preparations made on the basis of polysaccharides from the mushrooms, abroad are widely used for immunotherapy immunosuppressive States, as well as for prevention of immunodeficiency.

Study of the effect of the drug on cellular immunity conducted on 32 patients aged from 38 to 55 years old, living in the zone of increased radiation monitoring in the Kiev region. All patients at initial examination, there was a decline in the absolute number of lymphocytes. Control data obtained by the survey results 10 healthy individuals of the same age.

All examined patients were treated daily for 30 days 2 g of the proposed drug. Results changes in cellular immunity parameters in the observed patients are shown in table 11.

Table 11

Change indicators reflecting the state of cellular immunity when receiving medication
IndicatorsPatients before treatmentPatients after treatmentHealthy people
Eosinophils:
%2,6±0,81,9±0,51,5±0,2
Abscol in 1 ml104±44,761,3±30,546,2±12,1
Lim is ocity:
%34±4,340±4,737±2
Abscol in 1 ml1744±3162763±2473199,6±192
T-lymphocytes:
%29,4±and 7.742±9,753±3
Abscol in 1 ml468,9±118,51259,3±3421137±95,1
B-lymphocytes:
%13,2±1,716,9±2,920,5±1,8
Abscol in 1 ml234,1±62369±72449±60,9
Of cells:
%26,5±4,444,5±9,757,6±8,8
Abscol in 1 ml608±1081037±2641208,3±197

From the table it is seen that a daily intake of a new drug contributed to the increase in the absolute and relative number of lymphocytes, T-lymphocytes and b-lymphocytes. Along with this there is a tendency to reduce the number of eosinophils is the increase in the absolute number of b-lymphocytes. The analysis of each specific case of application of the drug showed that its effect was dependent on the initial state of the organism. At low indicators of the presence in blood of immunocompetent cells, the drug contributed to their increase at higher - lower, than showed an overall beneficial effect of this drug on the number and ratio of T - and b-lymphocytes.

The second property of the drug is its positive effect on the enzyme system of the liver. The state of detoxification of the liver determines the course of many diseases. Metabolic transformation of toxic products in the liver are carried out with the participation of the enzyme system of the endoplasmic reticulum of the liver. Detection of violations of the functional state of the oxidase-mixed liver is especially important for early diagnosis of possible disorders, and to assess the effectiveness of therapy.

The determination of the activity of oxidase-mixed liver function carried out by the method of Popov by using amidopirina (AP) as the test load on the body. The AP selection is regarded as a manifestation of adaptation to recurrent revenue of toxic substances in the body. Status oxidase-mixed liver to be judged by the content in the urine metabolites AP4-aminoantipyrine (4-AAP) and the product of its biological acetylation - 4-acetyl-4-aminoantipyrine (4-ICAAP), and their sum (SEE).

Load all the examined patients was conducted by a single reception amidopirina (AP) per os based 6 mg/kg of body weight, which corresponds to the average therapeutic dose and lower maximum single dose. This, along with years of experience in the use of AP as an antipyretic medication was the reason to consider a dose of 6 mg/kg completely harmless to the human body.

Identification of major metabolites amidopirina - 4-AAP and 4-ACAP, as well as their sum (CM) was conducted in 3-hour portion of urine (from 22 to 24 hours). The content of metabolites in the urine was determined by the colorimetric method, based on the formation of their indophenol red color by reaction with phenol in an alkaline medium in the presence of ferricyanide of potassium.

The results of determining the activity of the actions of enzymes involved in detoxification of toxic substances in the liver, obtained before and after the patients course of treatment, are shown in table 12.

Table 12

Change oxidase-mixed liver under the influence of medication
Group of patients (18)The excretion of metabolites in % of excretion AP
4-AARP 4-ACAPCM
Before the treatment0,58±0,0232,66±0,313,6±0,6
After the treatment0,40±being 0.0362,40±0,163,0±0,4
Decent indicators0,42±0,082,38±0,582,8±0,67

From the presented data shows that the increased allocation of AP metabolites and their amounts were observed in all patients (18) prior to taking the drug, which indicates considerable stress adaptation mechanisms in the surveyed persons.

The data obtained on the same patients at the end of the course of the drug indicate that the drug is statistically significantly contributed to the recovery detoxification of the liver. Positive therapeutic effect was achieved in all 18 patients have achieved complete recovery oxidase-mixed liver function.

Clinical studies of treatment-and-prophylactic properties of a new drug produced by biotechnological method on the basis of a new strain of the fungus Trametes pubescens showed that its normalizing effect on cellular immunity of the human body exceeds the same effect best drugs created for these purposes and that the drug helps restore the Department to physiological norms oxidase-mixed liver function.

1. The strain of the fungus Trametes pubescens VKPM F-839 - producer of ergosterol.

2. The drug has a positive effect on tissue metabolism, stimulating the immunogenesis, contributing to the restoration of disturbed oxidase-mixed liver, characterized in that it consists of the dried and separated from impurities biomass of the strain of the fungus Trametes pubescens VKPM F-839 and has the following composition, wt.%:

total protein40-50
carbohydrates25-30
including polysaccharides beta-glikana1-4
lipids3,5-5,0
including unsaturated fatty acids
phospholipids, ergosterol
nucleic acids3,0-4,5
minerals6,5-8,5
including calcium, potassium, phosphorus, magnesium,
iron, copper, zinc, chromium, cobalt, manganese, molybdenum
vitamins0,35-0,45
waterrest



 

Same patents:

FIELD: biotechnology, microbiology, pharmacology.

SUBSTANCE: invention relates to preparing a medicinal preparation using microorganism-producers. Lecanicillium sp. G16 strain is isolated from a soil sample from Teberdinsky reservation and deposited in Collection of microorganism cultures GNTSA at number 347A. Invention provides preparing a complex preparation possessing high hypolipidemic and antitumor activity and prolonged effect in low concentrations.

EFFECT: valuable medicinal properties of strain and preparation.

3 tbl, 1 ex

FIELD: agricultural microbiology, biotechnology.

SUBSTANCE: Penicillium vermiculatum Dang. PK-C fungus strain is prepared by selection way from Penicillium vermiculatum Dang. (VIZR-3) fungus strain and deposited in collection of pure cultures of All-Russian institute of plants protection at number VIZR-24. Proposed strain possesses high antagonistic activity against the broad spectrum of pathogens of diseases in plants. The maximal antagonistic activity of the strain is found against Sclerotinia sclerotiorum (Lib) de Bary, Phomopsis helianthi Munt.-Cvet., Michal., Petr., Botrytis cinerea Pers., and Verticillium dahliae Kleb. The strain possesses significant growth-stimulating activity increasing length and weight of seedling roots prepared from seeds treated with suspension of the proposed strain. The strain possesses resistance (compatibility) to some pesticides used for protection of sunflower, cereals, grape from complex of diseases and pests that allows using the biopreparation based on this strain in integrated system of plants protection. Invention can be used for production of biological agents used for control of phytopathogenic fungi.

EFFECT: valuable biological properties of fungus strain.

9 tbl, 8 ex

FIELD: medicine, microbiology, pharmacology, biotechnology.

SUBSTANCE: invention reports the strain Acremonium sp. G25 isolated from a soil sample in Teberdinsky reservation region and deposited in the Collection of cultures of microorganisms GNTSA at number 348A. Invention provides preparing the complex preparation possessing the high hypolipidemic and anti-tumor activity in the homeopathic concentrations. Invention can be used for preparing the medicinal preparation possessing high hypolipidemic and anti-tumor activity.

EFFECT: valuable medicinal properties of strain and preparation.

2 tbl, 1 ex

FIELD: biotechnology, microbiology.

SUBSTANCE: invention relates to a method for technological processing of lignocellulose materials. Method involves treatment of raw with enzyme preparation. As enzyme preparation method involves using a preparation obtained by culturing the strain Penicillium funiculosum S-2006 in medium containing the following components, g/l: cellulose, 50-60; glucose, 10-30; KH2PO4, 8-12; (NH4)2SO4, 3-7; MgSO4 x 7H2O, 0.2-0.4; CaCl2 x 2H2O, 0.2-0.4, and the process is carried out at temperature 26-30°C at the constant pH range 4.5-5.5. Treatment is carried out at intense stirring at temperature 45-55°C in weakly acid medium. The amount of enzyme preparation is selected by so manner to provides activity by filter paper 9-11 U per 1 g of dry substrate. Method provides the high yield of fermented sugars.

EFFECT: improved preparing method.

3 cl, 1 tbl

FIELD: biotechnology, microbiology, biochemistry.

SUBSTANCE: invention relates to development of a novel strain used for preparing enzyme representing a complex of acid subacid proteases. Strain is prepared by selection from the known strain Aspergillus oryzae (VKPM F-683) by multistep selection using effective methods of mutagenesis. Strain is stored as lyophilic dried culture and on slants with wort-agar in the biotechnology section of enzyme preparations in the food processing department of the State Scientific Institute VNII of food processing technology in Moscow. Invention provides preparing the strain possessing the high level of synthesis of acid and subacid proteases and high total activity of enzyme in cultural fluid exceeding activity of analogue by 2.0-2.6-fold and in reducing culturing process time.

EFFECT: improved and valuable properties of strain.

2 tbl, 5 ex

FIELD: biotechnology, microbiology, biochemistry.

SUBSTANCE: invention relates to the strain Aspergillus oryzae-12 providing the high level of synthesis of acid proteases and xylanase. The strain is obtained by multistep selection of the strain Aspergillus oryzae-387 (VKPM F-683) using effective methods of mutagenesis. The strain is deposited in collection VKPM at № F-932. Invention provides preparing the enzyme complex showing the high proteolytic and xylanolytic activity in cultural fluid wherein their level exceeds activity in analogue by 2.5-2.8-fold.

EFFECT: valuable properties of strain.

2 tbl, 3 ex

FIELD: biotechnology, microbiology, biochemistry.

SUBSTANCE: invention relates to the strain Aspergillus oryzae ROM-156 obtained by selection from the known strain Aspergillus oryzae-387 (VKPM F-683) by multistep selection using effective methods of mutagenesis. The strain is stored as lyophilic dried culture and on slants with wort-agar in section of biotechnology of enzyme preparations in food processing department of the State Scientific Institute VNII food processing technology in Moscow. Invention provides preparing the complex of proteinases and peptidases wherein their level of activity in cultural fluid exceeds activity of analogue by 6-10-fold, 1.3-1.4-fold and 1.6-1.9-fold with respect to β-glucanase, α-amylase and xylanase, respectively. The process cultivation time of the strain is reduced by 1.6-1.9-fold.

EFFECT: improved and valuable properties of strain.

3 tbl, 4 ex

FIELD: biotechnology, microbiology, biochemistry.

SUBSTANCE: invention relates to the strain Aspergillus oryzae-4150 obtained by selection from the known strain Aspergillus oryzae-387 (VKPM F-683) by multistep selection using effective methods of mutagenesis. The strain is stored as lyophilic dried culture and on slants with wort-agar in section of biotechnology of enzyme preparation, in food processing industry department of the State Scientific Institute of VNII food processing technology in Moscow. Invention provides preparing acid α-amylase showing high activity that exceeds activity in analogue by 2-3 times and reducing the process culturing time.

EFFECT: improved and valuable properties of strain.

2 tbl, 5 ex

FIELD: biotechnology.

SUBSTANCE: the present innovation deals with applying preparations for biological plant protection against soil larvae of snapping beetles. The suggested composition contains a solid carrier and a microbiological source of lysine, moreover as the solid carrier one should apply wheat grain and as lysine source - dry concentrate of fodder lysine and, also, a stock culture of entomopathogenic fungi.

EFFECT: higher efficiency of protection.

1 tbl

FIELD: chemical industry; mining industry; other industries; method of the biological purification of the sulfur-containing waste waters.

SUBSTANCE: the invention is pertaining to the method, which provides for the biological purification of the sulfur-containing waste waters. The biological purification of the sulfur-containing waste waters is exercised in the aerotank with usage of the micro-organisms-destructors. In the capacity of the micro-organisms- destructors use association of the strains Penicillium sp. №15 and Thiobacillus sp. №3 in the ratio 4:1. The degree of the biological purification of the sulfur-containing waste waters makes 85.2 %. The method allows to increase effectiveness of the biological purification of the sulfur-containing waste waters.

EFFECT: the invention ensures the increased effectiveness of the biological purification of the sulfur-containing waste waters.

3 dwg, 6 tbl, 6 ex

Compound // 2323940

FIELD: chemistry.

SUBSTANCE: photosensitising agents obtained by reducing a double bond in porphyrinic macrocycle of sulphonated mesotetraphenylporphyrine, preferably disulphonated mesotetraphenylporphyrine, such as TPPS2a. Resulting sulphonated mesotetraphenylchlorines are compounds of formula (I) , (where X stands for -SO3Н; each of n, p, q and r independently stands for 0 or 1; and sum of n, p, q and r is an integer from 1 to 4, preferably at least 2, in particular, 2 or 4), isomers or isomeric mixture. Compounds in accordance with the said invention and pharmaceutically suitable salts thereof have a high extinction coefficient in the region of 630 to 680 nm.

EFFECT: compounds are widely used as photosensitising agents for photochemical internalisation of molecules and photodynamic therapy.

25 cl, 8 ex, 8 dwg

FIELD: CHEMISTRY.

SUBSTANCE: crushed plant raw material with addition of ethanol modifier is extracted in two stages. At the first stage, supercritical carbon dioxide is used, which results in carbon dioxide extract further dissolved in ethanol by bubbling the extraction agent at atmospheric pressure. At the second stage, solvent cake is extracted with ethanol solution, which results in ethanolic extract.

EFFECT: invention increases grade of extraction of biologically active substances.

2 ex

FIELD: medicine; pharmacology.

SUBSTANCE: medicine for treatment of tuberculosis, contains the 30% alcoholic tincture of medicinal plants: knotgrass (Polygonum aviculare) herb, garden violet (Viola tricolor) herb, aspen (populus tremula) rind, agrimony (Agrimonia) herb, agrimony (Agrimonia) roots, rough-fruited cinquefoil (Potentilla recta) herb, golden thoroughwax (Bupleurum) herb, medic hop (Medicago lupulina) cone, Adonis vernalis herb, late red bartsia (Odontites vulgaris) herb, Betula leaf, bird cherry (Prunus padus) tree rind, common peony (Paeonia officinalis) root, common burdock (Arctium lappa) roots, foxtail clover (Trifolium rubens) bloom, cowberry (Comarum palustre) roots, Inula root, wheat grass (Agropyron) root, garden angelica (Angelica officinalis) root, coltsfoot herb, snowdrop anemone (Anemone sylvestris) herb, snowdrop anemone (Anemone sylvestris) root, locoweed semilunar (Astragalus) herb, arborescent aloe (Arborescence aloe) succus, swallowwort (Chelidonium majus) herb, black current (Ribes nigrum) leaf, garlic (Allium sativum) bulb, greater burnet (Sanguisorba officinalis) root, lady's finger (Lathyrus pratensis) herb, dandelion (Taraxacum officinale) root, stringing nettle (Urtica dioica) herb, common origanum (Origanum vulgare) herb, ginger plant (Tanacetum vulgare) herb, bottle brush (Equisetum arvense) herb, common wormwood (Artemisia absinthium) herb, crystal tea ledum (Ledum palustre) herb, common juniper (Juniperus communis) needle, parnassia herb, mother-of-thyme herb, flat-leaved snakeroot (Eryngium) herb, shinleaf (Ramischia secunda) herb, willow herb root and field pennycress (Thlaspi arvense) herb taken in specific proportion and added by 3 volume percent of 10% propolis tincture.

EFFECT: tincture shows efficiency in treating tuberculosis.

3 tbl

FIELD: medicine; pharmacology.

SUBSTANCE: medicine for treatment of tuberculosis, contains the 30% alcoholic tincture of medicinal plants: knotgrass (Polygonum aviculare) herb, garden violet (Viola tricolor) herb, aspen (populus tremula) rind, agrimony (Agrimonia) herb, agrimony (Agrimonia) roots, rough-fruited cinquefoil (Potentilla recta) herb, golden thoroughwax (Bupleurum) herb, medic hop (Medicago lupulina) cone, Adonis vernalis herb, late red bartsia (Odontites vulgaris) herb, Betula leaf, bird cherry (Prunus padus) tree rind, common peony (Paeonia officinalis) root, common burdock (Arctium lappa) roots, foxtail clover (Trifolium rubens) bloom, cowberry (Comarum palustre) roots, Inula root, wheat grass (Agropyron) root, garden angelica (Angelica officinalis) root, coltsfoot herb, snowdrop anemone (Anemone sylvestris) herb, snowdrop anemone (Anemone sylvestris) root, locoweed semilunar (Astragalus) herb, arborescent aloe (Arborescence aloe) succus, swallowwort (Chelidonium majus) herb, black current (Ribes nigrum) leaf, garlic (Allium sativum) bulb, greater burnet (Sanguisorba officinalis) root, lady's finger (Lathyrus pratensis) herb, dandelion (Taraxacum officinale) root, stringing nettle (Urtica dioica) herb, common origanum (Origanum vulgare) herb, ginger plant (Tanacetum vulgare) herb, bottle brush (Equisetum arvense) herb, common wormwood (Artemisia absinthium) herb, crystal tea ledum (Ledum palustre) herb, common juniper (Juniperus communis) needle, parnassia herb, mother-of-thyme herb, flat-leaved snakeroot (Eryngium) herb, shinleaf (Ramischia secunda) herb, willow herb root and field pennycress (Thlaspi arvense) herb taken in specific proportion and added by 3 volume percent of 10% propolis tincture.

EFFECT: tincture shows efficiency in treating tuberculosis.

3 tbl

FIELD: medicine; pharmacology.

SUBSTANCE: medicine for treatment of tuberculosis, contains the 30% alcoholic tincture of medicinal plants: knotgrass (Polygonum aviculare) herb, garden violet (Viola tricolor) herb, aspen (populus tremula) rind, agrimony (Agrimonia) herb, agrimony (Agrimonia) roots, rough-fruited cinquefoil (Potentilla recta) herb, golden thoroughwax (Bupleurum) herb, medic hop (Medicago lupulina) cone, Adonis vernalis herb, late red bartsia (Odontites vulgaris) herb, Betula leaf, bird cherry (Prunus padus) tree rind, common peony (Paeonia officinalis) root, common burdock (Arctium lappa) roots, foxtail clover (Trifolium rubens) bloom, cowberry (Comarum palustre) roots, Inula root, wheat grass (Agropyron) root, garden angelica (Angelica officinalis) root, coltsfoot herb, snowdrop anemone (Anemone sylvestris) herb, snowdrop anemone (Anemone sylvestris) root, locoweed semilunar (Astragalus) herb, arborescent aloe (Arborescence aloe) succus, swallowwort (Chelidonium majus) herb, black current (Ribes nigrum) leaf, garlic (Allium sativum) bulb, greater burnet (Sanguisorba officinalis) root, lady's finger (Lathyrus pratensis) herb, dandelion (Taraxacum officinale) root, stringing nettle (Urtica dioica) herb, common origanum (Origanum vulgare) herb, ginger plant (Tanacetum vulgare) herb, bottle brush (Equisetum arvense) herb, common wormwood (Artemisia absinthium) herb, crystal tea ledum (Ledum palustre) herb, common juniper (Juniperus communis) needle, parnassia herb, mother-of-thyme herb, flat-leaved snakeroot (Eryngium) herb, shinleaf (Ramischia secunda) herb, willow herb root and field pennycress (Thlaspi arvense) herb taken in specific proportion and added by 3 volume percent of 10% propolis tincture.

EFFECT: tincture shows efficiency in treating tuberculosis.

3 tbl

FIELD: medicine; pharmacology.

SUBSTANCE: medicine for treatment of tuberculosis, contains the 30% alcoholic tincture of medicinal plants: knotgrass (Polygonum aviculare) herb, garden violet (Viola tricolor) herb, aspen (populus tremula) rind, agrimony (Agrimonia) herb, agrimony (Agrimonia) roots, rough-fruited cinquefoil (Potentilla recta) herb, golden thoroughwax (Bupleurum) herb, medic hop (Medicago lupulina) cone, Adonis vernalis herb, late red bartsia (Odontites vulgaris) herb, Betula leaf, bird cherry (Prunus padus) tree rind, common peony (Paeonia officinalis) root, common burdock (Arctium lappa) roots, foxtail clover (Trifolium rubens) bloom, cowberry (Comarum palustre) roots, Inula root, wheat grass (Agropyron) root, garden angelica (Angelica officinalis) root, coltsfoot herb, snowdrop anemone (Anemone sylvestris) herb, snowdrop anemone (Anemone sylvestris) root, locoweed semilunar (Astragalus) herb, arborescent aloe (Arborescence aloe) succus, swallowwort (Chelidonium majus) herb, black current (Ribes nigrum) leaf, garlic (Allium sativum) bulb, greater burnet (Sanguisorba officinalis) root, lady's finger (Lathyrus pratensis) herb, dandelion (Taraxacum officinale) root, stringing nettle (Urtica dioica) herb, common origanum (Origanum vulgare) herb, ginger plant (Tanacetum vulgare) herb, bottle brush (Equisetum arvense) herb, common wormwood (Artemisia absinthium) herb, crystal tea ledum (Ledum palustre) herb, common juniper (Juniperus communis) needle, parnassia herb, mother-of-thyme herb, flat-leaved snakeroot (Eryngium) herb, shinleaf (Ramischia secunda) herb, willow herb root and field pennycress (Thlaspi arvense) herb taken in specific proportion and added by 3 volume percent of 10% propolis tincture.

EFFECT: tincture shows efficiency in treating tuberculosis.

3 tbl

FIELD: medicine; pharmacology.

SUBSTANCE: medicine for treatment of tuberculosis, contains the 30% alcoholic tincture of medicinal plants: knotgrass (Polygonum aviculare) herb, garden violet (Viola tricolor) herb, aspen (populus tremula) rind, agrimony (Agrimonia) herb, agrimony (Agrimonia) roots, rough-fruited cinquefoil (Potentilla recta) herb, golden thoroughwax (Bupleurum) herb, medic hop (Medicago lupulina) cone, Adonis vernalis herb, late red bartsia (Odontites vulgaris) herb, Betula leaf, bird cherry (Prunus padus) tree rind, common peony (Paeonia officinalis) root, common burdock (Arctium lappa) roots, foxtail clover (Trifolium rubens) bloom, cowberry (Comarum palustre) roots, Inula root, wheat grass (Agropyron) root, garden angelica (Angelica officinalis) root, coltsfoot herb, snowdrop anemone (Anemone sylvestris) herb, snowdrop anemone (Anemone sylvestris) root, locoweed semilunar (Astragalus) herb, arborescent aloe (Arborescence aloe) succus, swallowwort (Chelidonium majus) herb, black current (Ribes nigrum) leaf, garlic (Allium sativum) bulb, greater burnet (Sanguisorba officinalis) root, lady's finger (Lathyrus pratensis) herb, dandelion (Taraxacum officinale) root, stringing nettle (Urtica dioica) herb, common origanum (Origanum vulgare) herb, ginger plant (Tanacetum vulgare) herb, bottle brush (Equisetum arvense) herb, common wormwood (Artemisia absinthium) herb, crystal tea ledum (Ledum palustre) herb, common juniper (Juniperus communis) needle, parnassia herb, mother-of-thyme herb, flat-leaved snakeroot (Eryngium) herb, shinleaf (Ramischia secunda) herb, willow herb root and field pennycress (Thlaspi arvense) herb taken in specific proportion and added by 3 volume percent of 10% propolis tincture.

EFFECT: tincture shows efficiency in treating tuberculosis.

3 tbl

FIELD: medicine; pharmacology.

SUBSTANCE: medicine for treatment of tuberculosis, contains the 30% alcoholic tincture of medicinal plants: knotgrass (Polygonum aviculare) herb, garden violet (Viola tricolor) herb, aspen (populus tremula) rind, agrimony (Agrimonia) herb, agrimony (Agrimonia) roots, rough-fruited cinquefoil (Potentilla recta) herb, golden thoroughwax (Bupleurum) herb, medic hop (Medicago lupulina) cone, Adonis vernalis herb, late red bartsia (Odontites vulgaris) herb, Betula leaf, bird cherry (Prunus padus) tree rind, common peony (Paeonia officinalis) root, common burdock (Arctium lappa) roots, foxtail clover (Trifolium rubens) bloom, cowberry (Comarum palustre) roots, Inula root, wheat grass (Agropyron) root, garden angelica (Angelica officinalis) root, coltsfoot herb, snowdrop anemone (Anemone sylvestris) herb, snowdrop anemone (Anemone sylvestris) root, locoweed semilunar (Astragalus) herb, arborescent aloe (Arborescence aloe) succus, swallowwort (Chelidonium majus) herb, black current (Ribes nigrum) leaf, garlic (Allium sativum) bulb, greater burnet (Sanguisorba officinalis) root, lady's finger (Lathyrus pratensis) herb, dandelion (Taraxacum officinale) root, stringing nettle (Urtica dioica) herb, common origanum (Origanum vulgare) herb, ginger plant (Tanacetum vulgare) herb, bottle brush (Equisetum arvense) herb, common wormwood (Artemisia absinthium) herb, crystal tea ledum (Ledum palustre) herb, common juniper (Juniperus communis) needle, parnassia herb, mother-of-thyme herb, flat-leaved snakeroot (Eryngium) herb, shinleaf (Ramischia secunda) herb, willow herb root and field pennycress (Thlaspi arvense) herb taken in specific proportion and added by 3 volume percent of 10% propolis tincture.

EFFECT: tincture shows efficiency in treating tuberculosis.

3 tbl

FIELD: medicine; pharmacology.

SUBSTANCE: medicine for treatment of tuberculosis, contains the 30% alcoholic tincture of medicinal plants: knotgrass (Polygonum aviculare) herb, garden violet (Viola tricolor) herb, aspen (populus tremula) rind, agrimony (Agrimonia) herb, agrimony (Agrimonia) roots, rough-fruited cinquefoil (Potentilla recta) herb, golden thoroughwax (Bupleurum) herb, medic hop (Medicago lupulina) cone, Adonis vernalis herb, late red bartsia (Odontites vulgaris) herb, Betula leaf, bird cherry (Prunus padus) tree rind, common peony (Paeonia officinalis) root, common burdock (Arctium lappa) roots, foxtail clover (Trifolium rubens) bloom, cowberry (Comarum palustre) roots, Inula root, wheat grass (Agropyron) root, garden angelica (Angelica officinalis) root, coltsfoot herb, snowdrop anemone (Anemone sylvestris) herb, snowdrop anemone (Anemone sylvestris) root, locoweed semilunar (Astragalus) herb, arborescent aloe (Arborescence aloe) succus, swallowwort (Chelidonium majus) herb, black current (Ribes nigrum) leaf, garlic (Allium sativum) bulb, greater burnet (Sanguisorba officinalis) root, lady's finger (Lathyrus pratensis) herb, dandelion (Taraxacum officinale) root, stringing nettle (Urtica dioica) herb, common origanum (Origanum vulgare) herb, ginger plant (Tanacetum vulgare) herb, bottle brush (Equisetum arvense) herb, common wormwood (Artemisia absinthium) herb, crystal tea ledum (Ledum palustre) herb, common juniper (Juniperus communis) needle, parnassia herb, mother-of-thyme herb, flat-leaved snakeroot (Eryngium) herb, shinleaf (Ramischia secunda) herb, willow herb root and field pennycress (Thlaspi arvense) herb taken in specific proportion and added by 3 volume percent of 10% propolis tincture.

EFFECT: tincture shows efficiency in treating tuberculosis.

3 tbl

FIELD: medicine; pharmacology.

SUBSTANCE: medicine for treatment of tuberculosis, contains the 30% alcoholic tincture of medicinal plants: knotgrass (Polygonum aviculare) herb, garden violet (Viola tricolor) herb, aspen (populus tremula) rind, agrimony (Agrimonia) herb, agrimony (Agrimonia) roots, rough-fruited cinquefoil (Potentilla recta) herb, golden thoroughwax (Bupleurum) herb, medic hop (Medicago lupulina) cone, Adonis vernalis herb, late red bartsia (Odontites vulgaris) herb, Betula leaf, bird cherry (Prunus padus) tree rind, common peony (Paeonia officinalis) root, common burdock (Arctium lappa) roots, foxtail clover (Trifolium rubens) bloom, cowberry (Comarum palustre) roots, Inula root, wheat grass (Agropyron) root, garden angelica (Angelica officinalis) root, coltsfoot herb, snowdrop anemone (Anemone sylvestris) herb, snowdrop anemone (Anemone sylvestris) root, locoweed semilunar (Astragalus) herb, arborescent aloe (Arborescence aloe) succus, swallowwort (Chelidonium majus) herb, black current (Ribes nigrum) leaf, garlic (Allium sativum) bulb, greater burnet (Sanguisorba officinalis) root, lady's finger (Lathyrus pratensis) herb, dandelion (Taraxacum officinale) root, stringing nettle (Urtica dioica) herb, common origanum (Origanum vulgare) herb, ginger plant (Tanacetum vulgare) herb, bottle brush (Equisetum arvense) herb, common wormwood (Artemisia absinthium) herb, crystal tea ledum (Ledum palustre) herb, common juniper (Juniperus communis) needle, parnassia herb, mother-of-thyme herb, flat-leaved snakeroot (Eryngium) herb, shinleaf (Ramischia secunda) herb, willow herb root and field pennycress (Thlaspi arvense) herb taken in specific proportion and added by 3 volume percent of 10% propolis tincture.

EFFECT: tincture shows efficiency in treating tuberculosis.

3 tbl

FIELD: medicine, oncology, amino acids.

SUBSTANCE: invention relates, in particular, to the development of an antitumor preparation based on natural substances. Invention relates to an amino acid preparation comprising at least one modified essential amino acid obtained by treatment of amino acid by ultraviolet radiation (UV) at wavelength 250-350 nm for 12-80 h at temperature 15-30oC or with ozone at temperature 15-25oC. The modified amino acid has no toxicity for health cells. Also, invention relates to a method for preparing such preparation. Invention provides the development of an antitumor preparation based on modified amino acids and expanded assortment of antitumor preparations being without cytotoxicity for normal cells.

EFFECT: valuable medicinal antitumor properties of preparation.

8 cl, 4 tbl, 2 dwg, 4 ex

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