Human antibody possessing binding activity with mn and neutralization of cellular adhesion

FIELD: immunology, antibodies.

SUBSTANCE: invention elates to human monoclonal antibodies to MN and antibody fragments to MN that are targeted to repeat sequence GEEDLP within proteoglycan domain. Binding with a desired epitope is confirmed by competitive immunoenzyme analysis method ELISA wherein ELISA signal is attenuated in combined incubation with peptide comprising this repeat sequence (PGEEDLPGEEDLP). Binding inhibition can be confirmed by the Biacore study also wherein binding required antibodies with immobilized MN or proteoglycan peptides can be inhibited by peptide repeat sequence. In addition to binding with human peptide repeat sequence anti-MN can inhibit adhesion of CGL-1 cells to plastic plates covered by MN. Human antibodies anti-MN can be used in treatment of cancer diseases or for diagnosis of cancer diseases wherein the level of MN is increased.

EFFECT: valuable medicinal properties of antibodies.

11 cl, 8 dwg, 2 tbl, 13 ex

 

The text descriptions are given in facsimile form.

1. The purified preparation of human antibodies containing at least one region selected from the group comprising VH3 region-CDR3-containing amino acid sequence selected from the group comprising the sequences SEQ ID NOS: 61-80, VH3 region-CDR1-containing amino acid sequence selected from the group comprising the sequences SEQ ID NOS: 48-60, region VLλ1-CDR3-containing amino acid sequence under the number SEQ ID NO: 81, region VLλ2-CDR1-containing amino acid sequence selected from the group comprising sequence SEQ ID NOS: 82-83, or region VLλ2-CDR3-containing amino acid sequence selected from the group comprising the sequences SEQ ID NOS: 84-89, with the indicated antibody binds to a protein MN.

2. The purified preparation according to claim 1, whereby the antibody binds to proteoglycans domain protein MN.

3. The purified preparation according to claim 1, whereby the antibody binds to the repeat region GEEDLP inside proteoglycan domain protein MN.

4. The purified preparation according to claim 3, whereby the antibody binds with protein MN person with Kdfrom about 0.6 to about 1800 nm.

5. The purified preparation according to claim 3, and as is titulo binds with protein MN person with K dfrom about 0.6 to about 90 nm.

6. The purified preparation of claim 1, wherein the human antibody contains the VH3-CDR3 containing the amino acid sequence of SEQ ID NO: 64.

7. The purified preparation of claim 1, wherein the human antibody comprises a pair of amino acid sequence VH3-CDR3 and VL2-CDR3 selected from the group comprising the sequences SEQ ID NOS: 61 and 84, SEQ ID NOS: 62 and 87, SEQ ID NOS: 63 and 89, SEQ ID NOS: 64 and 84, SEQ ID NOS: 65 and 84, SEQ ID NOS: 66 and 85, SEQ ID NOS: 67 and 88.

8. The purified preparation of claim 1, wherein the human antibody comprises a pair of amino acid sequence VH3-CDR3 and VL2-CDR3 selected from the group comprising the sequences SEQ ID NOS: 61 and 86, SEQ ID NOS: 61 and 85, SEQ ID NOS: 61 and 87, SEQ ID NOS: 61 and 88, SEQ ID NOS: 61 and 89, SEQ ID NOS: 63 and 86, SEQ ID NOS: 63 and 85. SEQ ID NOS: 63 and 87, SEQ ID NOS: 63 and 88, SEQ ID NOS: 63 and 84.

9. The purified preparation of claim 1, wherein the human antibody comprises a pair of amino acid sequence VH3-CDR3 and VL2-CDR3 selected from the group comprising the sequences SEQ ID NOS: 71 and 87, SEQ ID NOS: 61 and 87, SEQ ID NOS: 72 and 87, SEQ ID 73 and 87, SEQ ID NOS: 74 and 87, SEQ ID NOS: 75 and 87, SEQ ID NOS: 76 and 87, SEQ ID NOS: 77 and 87, SEQ ID NOS: 78 and 87, SEQ ID NOS: 79 and 87, SEQ ID NOS: 80 and 87.

10. The purified preparation of claim 1, the human antibody comprises a pair of amino acid sequence VH3-CDR3 and VL1-CDR3 selected from the group comprising the sequences SEQ ID NOS: 61 and 81, SEQ ID NOS: 69 and 81, SEQ ID NOS: 70 and 81.

11. The purified preparation of claim 1, the antibody of the person contains minocyclinee sequence VH3-CDR3, VL2-CDR3 and VH3-CDR1 selected from the group comprising the sequences SEQ ID NOS: 61 and 86 and 48, SEQ ID NOS: 61 and 86 and 49, SEQ ID NOS: 61 and 86 and 50, SEQ ID NOS: 61 and 86 and 51, SEQ ID NOS: 61 and 86 and 52, SEQ ID NOS: 61 and 86 and 53, SEQ ID NOS: 61 and 86 and 54, SEQ ID NOS: 61 and 86 and 55, SEQ ID NOS: 61 and 86 and 56, SEQ ID NOS: 61 and 86 and 57.

Priority points and features:

18.10.2001 according to claim 2; 3; 6-8; 10; 1 part of SEQ ID NO: 61-70, 81 and 84-89; 4 in part 4,7-1800 nm; 5 in part 4-90 nm; 9 in part of SEQ ID NO: 61 and 87; 11 in part of SEQ ID NO: 61 and 86;

02.05.2002 according to claim 1 in part of SEQ ID NO: 52-60; 4 and 5 in part 0,6 nm; 11 in parts of SEQ ID nos: 52-57;

18.10.2002 according to claim 1 in part of SEQ ID NO: 48-51, 71-80 and 82-83; 4 parts of 0.6<Kd<a 4.7 nm; 5 parts of 0.6<Kd<4 nm; 9 in part of SEQ ID NO: 71-80; 11 in part of SEQ ID NO: 48-51.



 

Same patents:

FIELD: oncology and biotechnology.

SUBSTANCE: invention concerns conjugates used for treatment of malignant tumor. Conjugate includes staphylococcal or streptococcal wild-type superantigen or modified superantigen and antibody constituent. Bacterial superantigen is modified to reduce serum reactivity with preserved its antigenic activity. Amino acid sequence of superantigen incorporates A-E regions determining binding to TCR and MHC molecules class II. Invention is directed to preparing antitumor drug and also to preparing pharmaceutical composition.

EFFECT: use of the conjugate according to invention activate immune system and, therefore, resistance of mammalian against malignant tumor.

67 cl, 11 dwg, 1 tbl, 11 ex

FIELD: biotechnology, peptides.

SUBSTANCE: invention relates to a method for preparing antibodies raised to human leukocyte differentiation factor (HLDF) or to HLDF fragment (31-38) representing peptide of the following structure: Arg-Arg-Trp-His-Arg-Leu-Glu-Lys possessing with antigenic and nucleic acids-hydrolyzing properties, and for diagnostic aims also. Antibodies are prepared from rabbit plasma blood immunized with three injections of antigens wherein synthetic HLDF factor or conjugate is used as antigens. Diagnosis of anaplastic state of human cells is carried out by using solutions of antibodies to HLDF factor or HLDF fragment (31-38) in the concentration 0.0013 mg/ml as biological markers. Invention provides carrying out the differential diagnosis of tumors and normal organs and effective detecting initial stages in cell differentiation disturbances.

EFFECT: improved preparing method of antibody, improved method for diagnosis.

6 cl, 21 dwg, 1 tbl

FIELD: medicine, oncology, biochemistry.

SUBSTANCE: invention relates to fused proteins, namely to the multifunctional fused protein cytokine-antibody. This fused protein involves immunoglobulin region and cytokine fused protein of the formula IL-12-X or X-IL-12 wherein interleukin-12 (IL-12) represents the first cytokine and X represents the second cytokine taken among the group comprising IL-2, IL-4 and GM-CSF bound covalently either by amino-end or carboxyl-end to subunit p35 or p40 of interleukin-12 (IL-12) in its heterodimeric or a single-chain form. Indicated fused cytokine protein is fused by either its amino-end or carboxyl-end with indicated region of immunoglobulin. Multifunctional fused protein cytokine-antibody shows an anticancer activity.

EFFECT: valuable medicinal properties of protein complexes.

13 cl, 40 dwg, 18 ex

The invention relates to the field of immunobiotechnology and may find application in medicine

FIELD: biotechnology, genetic engineering.

SUBSTANCE: invention describes recombinant plasmid DNAs constructed in vitro that comprise artificial genes for light and heavy chains of full-scale human antibody prepared by genetic engineering methods. These genes are created on basis of variable fragments of light and heavy chains of recombinant antibody 1F4 and constant human genes IgG1, cytomegalovirus promoter and polyadenylation site BGH. Plasmids provide biosynthesis of recombinant full-scale human antibodies of class IgG1 in mammalian cells. These antibodies interact specifically with smallpox vaccine virus. The affinity constant for prepared recombinant antibodies is 3.54 x 109 ± 0.38 x 109 M-1. Plasmids are used by combined transfection of human cells HEK 293T. Prepared full-scale recombinant antibody against protein of size 27 kDa of smallpox virus vaccine can be used as a base for creature of pharmaceutical preparations used for diagnosis of some post-vaccine complications caused by smallpox virus vaccine. Also, preparations will comprise decreased therapeutic doses of immunoglobulins that will provide minimal undesirable immune response in patients after administration of the preparation.

EFFECT: valuable medicinal properties of plasmid DNA.

4 cl, 7 dwg, 6 ex

FIELD: biotechnology, genetic engineering.

SUBSTANCE: invention describes recombinant plasmid DNAs constructed in vitro that comprise artificial genes for light and heavy chains of full-scale human antibody prepared by genetic engineering methods. These genes are created on basis of variable fragments of light and heavy chains of recombinant antibody 1F4 and constant human genes IgG1, cytomegalovirus promoter and polyadenylation site BGH. Plasmids provide biosynthesis of recombinant full-scale human antibodies of class IgG1 in mammalian cells. These antibodies interact specifically with smallpox vaccine virus. The affinity constant for prepared recombinant antibodies is 3.54 x 109 ± 0.38 x 109 M-1. Plasmids are used by combined transfection of human cells HEK 293T. Prepared full-scale recombinant antibody against protein of size 27 kDa of smallpox virus vaccine can be used as a base for creature of pharmaceutical preparations used for diagnosis of some post-vaccine complications caused by smallpox virus vaccine. Also, preparations will comprise decreased therapeutic doses of immunoglobulins that will provide minimal undesirable immune response in patients after administration of the preparation.

EFFECT: valuable medicinal properties of plasmid DNA.

4 cl, 7 dwg, 6 ex

FIELD: biotechnology, molecular biology.

SUBSTANCE: invention proposes a polynucleotide VEGI-192a encoding polypeptide that inhibits growth of human vascular endothelial cells. Invention describes expressing vector comprising polynucleotide and E. coli cell-host comprising vector. Invention discloses polypeptide encoded by polynucleotide and fused protein based on indicated polypeptide. Invention describes polynucleotide encoding fused protein and expressing vector based on indicated polynucleotide. Invention discloses a pharmaceutical composition used for inhibition of angiogenesis based on polypeptide-inhibitor of growth of human vascular endothelial cells and polynucleotide encoding its. Invention describes therapeutic methods for inhibition of angiogenesis and suppression of tumor growth based on this composition. Invention describes an antibody raised to polypeptide that inhibits growth of human vascular endothelial cells. Using this invention provides novel forms of inhibitor of human growth of vascular endothelial cells and can be used in medicine.

EFFECT: valuable biological and medicinal properties of inhibitor.

27 cl, 27 dwg, 13 tbl, 34 ex

FIELD: medicine, oncology, genetic engineering, pharmacy.

SUBSTANCE: invention relates to a peptide or polypeptide representing a Fv-molecule, its genetic engineering construction, fragment of Fv-molecule or its genetic engineering construction able to bind leucosis cells and cells expressing glycocalicine. The selective and/or specific binding with target-cell is determined firstly by the first hypervariable site being Fv-molecule is a single-chain Fv-molecule or disulfide Fv-molecule (scFv or dsFv) and can be labeled by one or more labels. Fv molecule comprises variable sites of heavy and light chain wherein variable site of heavy chain comprises CDR3, CDR2 and CDR1 sites with amino acids sequences given in the invention description. Invention provides using peptides or polypeptides for design of an antitumor pharmaceutical compositions based on the specific direction for enhanced binding on essentially exposed and/or superexpressed binding site in target-cell or inside of its. Also, binding with a target-cell is carried out for benefit of other cells on which or inside of these cells this binding site is not essentially available and/or expressed.

EFFECT: valuable biological and medicinal properties of antibodies.

11 cl, 25 dwg, 11 tbl, 10 ex

FIELD: medicine, biotechnology.

SUBSTANCE: invention proposes variants of antibodies showing specificity to peptide domain located by both side of hinged site R76S77 in pro-BNP(1-108). Indicated antibodies recognize specifically also circulating pro-BNP(1-108) in human serum or plasma samples but they don't recognize practically peptides BNP(1-76) or BNP(77-108). Also, invention describes variants of peptides used in preparing antibodies. Amino acid sequence is given in the invention description. Also, invention discloses methods for preparing indicated antibodies and among of them by using indicated peptides. Also, invention describes methods for preparing antibody-secreting hybridoma, and hybridoma is disclosed prepared by indicated method. Also, invention describes a monoclonal antibody secreted by hybridoma 3D4 and deposited at number CNCM I-3073. Also, invention discloses variants for diagnosis of cardiac insufficiency in vitro and by using antibodies proposed by the invention. Also, invention describes a set used for detecting pro-BNP(1-108) in a biological sample. Using this invention simplifies detection of pro-BNP(1-108) circulating in human serum or plasma samples and provides specific detection of pro-BNP(1-108) that can be used in early diagnosis of human cardiac insufficiency.

EFFECT: valuable medicinal properties of antibodies.

24 cl, 16 dwg, 5 tbl, 20 ex

FIELD: medicine, biotechnology.

SUBSTANCE: invention proposes variants of antibodies showing specificity to peptide domain located by both side of hinged site R76S77 in pro-BNP(1-108). Indicated antibodies recognize specifically also circulating pro-BNP(1-108) in human serum or plasma samples but they don't recognize practically peptides BNP(1-76) or BNP(77-108). Also, invention describes variants of peptides used in preparing antibodies. Amino acid sequence is given in the invention description. Also, invention discloses methods for preparing indicated antibodies and among of them by using indicated peptides. Also, invention describes methods for preparing antibody-secreting hybridoma, and hybridoma is disclosed prepared by indicated method. Also, invention describes a monoclonal antibody secreted by hybridoma 3D4 and deposited at number CNCM I-3073. Also, invention discloses variants for diagnosis of cardiac insufficiency in vitro and by using antibodies proposed by the invention. Also, invention describes a set used for detecting pro-BNP(1-108) in a biological sample. Using this invention simplifies detection of pro-BNP(1-108) circulating in human serum or plasma samples and provides specific detection of pro-BNP(1-108) that can be used in early diagnosis of human cardiac insufficiency.

EFFECT: valuable medicinal properties of antibodies.

24 cl, 16 dwg, 5 tbl, 20 ex

FIELD: biotechnology, immunology.

SUBSTANCE: invention describes a monoclonal anti-IFNα antibody that binds with the following subtypes of IFNα: IFNα1, IFNα2, IFNα4, IFNα5, IFNα8, IFNα10 and IFNα21 and comprises three CDR-sites of heavy chain. Amino acid is given in the invention description. Invention discloses heavy chain of anti-IFNα antibody or its fragment that comprise indicated CDR-sites also. Invention describes anti-IFNα antibody that comprises at least one light chain and one heavy chain. Invention discloses variants of nucleic acids encoding indicated antibodies and variants of vectors used for expression of nucleic acids, and variants of transformed host-cells. Among expression vectors invention describes also vectors deposited at № 2881 and № 2882 carrying heavy and light chain of antibody, respectively. Invention describes a method for preparing antibody from indicated cells. Invention discloses the murine hybridoma cell line deposited in ATCC at number № РТА-2917, and antibody produced by indicated cell line. Also, invention describes variants of the antibody-base pharmaceutical composition and a method used for diagnosis of autoimmune disease. Also, invention discloses using antibodies in treatment of disease or state associated with enhanced level of IFNα in a patient. Using the invention provides inhibiting biological activity of at least seven human IFNα subtypes simultaneously, namely: IFNα1, IFNα2, IFNα4, IFNα5, IFNα8, IFNα10 and IFNα12 that can be used in diagnosis and therapy of different human diseases mediated by IFNα, such as insulin-dependent diabetes mellitus or erythematosus lupus.

EFFECT: valuable biological and medicinal properties of antibodies.

53 cl, 4 tbl, 10 dwg, 2 ex

FIELD: biotechnology, immunology.

SUBSTANCE: invention describes a monoclonal anti-IFNα antibody that binds with the following subtypes of IFNα: IFNα1, IFNα2, IFNα4, IFNα5, IFNα8, IFNα10 and IFNα21 and comprises three CDR-sites of heavy chain. Amino acid is given in the invention description. Invention discloses heavy chain of anti-IFNα antibody or its fragment that comprise indicated CDR-sites also. Invention describes anti-IFNα antibody that comprises at least one light chain and one heavy chain. Invention discloses variants of nucleic acids encoding indicated antibodies and variants of vectors used for expression of nucleic acids, and variants of transformed host-cells. Among expression vectors invention describes also vectors deposited at № 2881 and № 2882 carrying heavy and light chain of antibody, respectively. Invention describes a method for preparing antibody from indicated cells. Invention discloses the murine hybridoma cell line deposited in ATCC at number № РТА-2917, and antibody produced by indicated cell line. Also, invention describes variants of the antibody-base pharmaceutical composition and a method used for diagnosis of autoimmune disease. Also, invention discloses using antibodies in treatment of disease or state associated with enhanced level of IFNα in a patient. Using the invention provides inhibiting biological activity of at least seven human IFNα subtypes simultaneously, namely: IFNα1, IFNα2, IFNα4, IFNα5, IFNα8, IFNα10 and IFNα12 that can be used in diagnosis and therapy of different human diseases mediated by IFNα, such as insulin-dependent diabetes mellitus or erythematosus lupus.

EFFECT: valuable biological and medicinal properties of antibodies.

53 cl, 4 tbl, 10 dwg, 2 ex

FIELD: biotechnology, immunology, molecular biology, pharmacy.

SUBSTANCE: invention describes variants of MCP-1-binding molecules. One of MCP-1-binding molecule comprises at least one variable region of immunoglobulin (VH) heavy chain comprising of hypervariable sites CDR1, CDR2 and CDR3 while other molecules comprises both light and heavy chains. Invention proposes DNA constructs encoding indicated MCP-1-binding molecules and expressing vector carrying at least one of these DNA constructs. Invention describes a method for preparing MCP-1-binding molecule. Invention discloses a method for treatment of disease or disorder mediated by MCP-1 or eotaxine-1 based on antibody raised to MCP-1 that binds eotaxine-1 by cross mode. Invention describes a pharmaceutical composition based on antibody raised to MCP-1 that binds eotaxine-1 by cross mode and used in treatment of disease or disorder mediated by MCP-1 or eotaxine-1 in a patient. MCP-1-binding molecules inhibit binding MCP-1 with its receptor. The full immobilized antibody is highly specific as far as it binds human recombinant MCP-1 with value KD = (43 ± 2.9) x 1012 and can be used in medicine.

EFFECT: valuable medicinal properties of antibodies, improved method of treatment.

13 cl, 5 dwg, 4 tbl, 2 ex

FIELD: biotechnology, immunology, medicine, oncology.

SUBSTANCE: invention describes variants of monoclonal antibodies showing specificity to TRAIL-receptor DR4. By one of variant antibodies are produced by hybridoma 2E12 recorded in ATCC at number PTA-3798. Each of antibody variants possesses apoptosis-inducing activity both in vivo - in the concentrations less 10 mg/kg in target cells expressing DR4 and in vitro - in the presence of a cross-linking agent in the concentrations less 1 mcg/ml in target cells. Invention discloses variants of methods for selective induction of apoptosis in cells expressing DR4, and variants of methods for inhibition of DR4-expressing cells based on using antibodies. Invention describes variants of compositions, methods for treatment of a patient suffering from inflammatory or autoimmune disease and methods in treatment of a patient suffering from malignant tumor wherein these compositions are based on antibody to DR4 for inducing apoptosis in cells expressing DR4. Also, invention discloses variants of nucleic acids, purified polypeptides, expression vectors and host-cells used in preparing antibody. Using the invention provides delaying tumor growth and decreasing case of its regression that can be used in tumor therapy.

EFFECT: improved and valuable properties of antibody.

103 cl, 169 dwg, 6 tbl, 30 ex

FIELD: genetic engineering, immunology, medicine.

SUBSTANCE: invention relates to new antibodies directed against antigenic complex CD3 and can be used in therapeutic aims. Antibody IgG elicits the affinity binding with respect to antigenic complex CD3 wherein heavy chain comprises skeleton of the human variable region in common with at least one CD3 taken among amino acid sequences SEQ ID NO 2, 4 and 6 and their corresponding conservatively modified variants. Light chain comprises skeleton of the rodent variable region in common with at least one CD3 taken among amino acid sequences SEQ ID NO 8, 10 and 12 and their corresponding conservatively modified variants. Antibody is prepared by culturing procaryotic or eucaryotic cell co-transformed with vector comprising recombinant nucleic acid that encodes antibody light chain and vector comprising recombinant nucleic acid that encodes antibody heavy chain. Antibody is administrated in the patient suffering with malignant tumor or needing in immunosuppression in the effective dose. Invention provides preparing chimeric antibodies against CD3 that are produced by expression systems of procaryotic and eucaryotic cells with the enhanced yield.

EFFECT: improved preparing methods, valuable medicinal properties of antibody.

33 cl, 5 dwg, 1 ex

FIELD: genetic engineering, immunology, medicine.

SUBSTANCE: invention relates to new antibodies directed against antigenic complex CD3 and can be used in therapeutic aims. Antibody IgG elicits the affinity binding with respect to antigenic complex CD3 wherein heavy chain comprises skeleton of the human variable region in common with at least one CD3 taken among amino acid sequences SEQ ID NO 2, 4 and 6 and their corresponding conservatively modified variants. Light chain comprises skeleton of the rodent variable region in common with at least one CD3 taken among amino acid sequences SEQ ID NO 8, 10 and 12 and their corresponding conservatively modified variants. Antibody is prepared by culturing procaryotic or eucaryotic cell co-transformed with vector comprising recombinant nucleic acid that encodes antibody light chain and vector comprising recombinant nucleic acid that encodes antibody heavy chain. Antibody is administrated in the patient suffering with malignant tumor or needing in immunosuppression in the effective dose. Invention provides preparing chimeric antibodies against CD3 that are produced by expression systems of procaryotic and eucaryotic cells with the enhanced yield.

EFFECT: improved preparing methods, valuable medicinal properties of antibody.

33 cl, 5 dwg, 1 ex

FIELD: biotechnology, medicine, proteins.

SUBSTANCE: invention describes new polypeptide in isolated form relating to subfamily of superfamily human immunoglobulins (Ig-Sf). This polypeptide shows at least 70% of homology level with amino acid sequence of murine molecules CRAM-1 or CRAM-2 regulated by the confluence of adhesive (figures 3, 6 are represented in the claim). Also, invention relates to antibodies showing specificity with respect to the polypeptide. Antibodies and soluble polypeptide can be used for treatment of inflammation and tumors. Invention describes polynucleotide or oligonucleotide encoding the full-size polypeptide or its moiety and represents primer, probe, anti-sense RNA and shows the nucleotide sequence that is identical conceptually with human CRAM-1. Invention provides preparing new adhesive proteins from superfamily Ig-Sf that are regulated at the transcription level in endothelium by effect of tumors. Invention can be used for treatment of different diseases, in particular, inflammatory responses.

EFFECT: valuable medicinal properties of polypeptide.

19 cl, 33 dwg, 1 ex

FIELD: biotechnology, vaccines.

SUBSTANCE: invention proposes an oligomeric particle that is able to induce immunity against hepatitis C virus. Particle consists of purified proteins or their parts, hepatitis C virus envelope (HCV) E1, E1s and E2. Proposed particle has diameter from 5 to 100 nm. Also, invention proposes a method for preparing such particle. Method involves purification of proteins HCV in solution, formation of oligomeric particles by replacing purified proteins with detergent solution or salt solution and purification of oligomeric particles. Also, invention proposes a composition for inducing immunity against HCV that comprises the proposed particle, antibody specific to particle, sets for detection of antigens HCV, immunological analysis for detection of antibodies to HCV, and vaccine against hepatitis C virus (HCV) that comprises the oligomeric particle. Proposed inventions provide carrying out the induction of specific humoral and cellular immunity to HCV envelope proteins and to carry out diagnosis of HCV. Invention can be used in medicine.

EFFECT: valuable medicinal properties of particle, improved preparing methods.

71 cl, 27 dwg, 6 tbl, 14 ex

FIELD: microbiology and immunology, in particular immunodiagnosis.

SUBSTANCE: atypical strain of melioidose Burkholderia pseudomallei-111-6-1 with altered phenotype defected with respect to synthesis of 8 antigen and acting as immunosuppressor is used as antigen for animal immunization. Immune serum is obtained after 2 immunization cycles of animal-producer with titer in gel immunodiffusion reaction not less than 1:128.

EFFECT: immune serum with increased specific activity.

2 tbl, 2 ex

FIELD: genetic engineering, in particular genes for cell cycle controlling point.

SUBSTANCE: polynucleotide encoding rad3 polypeptide ATR homologue is cloned into expression vector, having functionality in eucariotic cells. Polypeptide of rad3 polypeptide ATR homologue is obtained by cultivation of eucariotic cell culture, transformed by vector. Monoclonal antibody to rad3 polypeptide ATR homologue is obtained by hybridoma technologies. Polyclonal antibodies are obtained by inoculation of rad3 polypeptide ATR homologue in host animal. Polynucleotide presence in animal tissue sample is detected by contacting of this sample containing DNA or RNA with polynucleotide encoding rad3 polypeptide ATR homologue under hybridization conditions. Polypeptide in biological sample is detected by sample contact with monoclonal or polyclonal antibodies. Substances having anticancer activity are screened on the base of reduced activity of ATR polypeptide on substrate or reduced chelating of ATR homologue in presence of candidate substance. Present invention makes it possible to produce human or S.pombe rad3 polypeptide ATR homologue and is useful in investigation ATR role as gene for cell cycle controlling point in cell culture in vivo or in vitro.

EFFECT: new anticancer substances.

24 cl, 1 dwg

Up!