Associate containing trehalose and calcium chloride as crystalline monohydrate or anhydrous crystal

FIELD: cosmetics, pharmacy.

SUBSTANCE: invention relates to associates containing trehalose and calcium chloride as a crystalline monohydrate comprising above said components and water in the molar ratio = 1:1:1, or as anhydrous crystal containing above said components in the molar ratio = 1:2. Indicated crystals of two species of associates of trehalose and calcium chloride can be used in different branches in production of foodstuffs, cosmetic agents and others.

EFFECT: valuable properties of compounds.

3 cl, 20 tbl, 15 dwg, 30 ex

 

The technical field

The present invention relates to new associates of sugars and compounds containing metal ions, and more particularly to associates of trehalose or maltitol and compounds containing metal ions.

Description of the prior art,

Compared to carbon, oxygen, hydrogen, nitrogen, etc. metal elements such as sodium, potassium, calcium, magnesium, iron, copper, zinc, Nickel, etc. are not required in large quantities for living organisms. However, they are essential elements to maintain normal biological functions. Such metal elements are usually introduced into living organisms in the form of compounds, including ionized metal elements (compounds containing metal ions, or ion-metal compounds, such as salts, and perform the corresponding functions in organisms.

It is known that magnesium and calcium are minerals that are included in enzymatic reactions in the human organisms and are required in relatively large amounts. Deficiency of magnesium and calcium causes thinning of the bones due to osteoporosis and osteomalacia, because these metal elements are present in relatively large amounts in the bones of living organisms. Recently, deficiency of magnesium was rizana as the cause of diseases, such as diabetes and hypertension.

Magnesium is an essential mineral for plants and generally is delivered to plants in the form of fertilizers with nitrogen, phosphorus and potassium in liquid or solid form and is used for their growth. It is known that a lack of magnesium causes a disease associated with deficiency. Although, as described above, various metallic elements are essential to living organisms, there is a case where compounds containing metal ions, such as salts, causing an unpleasant taste when administered orally, depending on quantity. So the problem compounds containing metal ions, in the food industry recognized mainly because of the unpleasant taste of these compounds when administered orally, and to resolve this problem have been various studies.

The authors of the present invention is widely investigated mainly the development and use of new food materials with a focus on sugars and substances associated with sugars. As part of their research the authors of the present invention investigated the composition, including sugars and compounds containing metal ions (ion-metal connection) to identify new and effective uses in the food industry. When you study the years of the authors of the present invention found the fact that what compounds containing metal ions commonly used in the production, storage of food products or their raw materials, have unfavorable properties, such as blurring in the humid air due to hygroscopicity, healing ability, the oxidizing capacity, low solubility in water and so on; the inventors have noted that, as is usually assumed, adverse properties characteristic of compounds containing metal ions, and cannot be improved; and that these problems are not recognized as problems to be solved. The authors of the present invention considered that the development of drug compounds containing metal ions, with improved unfavorable characteristics and their delivery would have made a great contribution to the food industry.

The present invention solves a fairly new problem that arose on the basis of the original concept authors present invention by the provision of drugs, including compounds containing metal ions or components uterine saline, whose inherent unfavorable for industrial use properties, such as blurring in the humid air due to hygroscopicity, healing ability, the oxidizing capacity, low solubility in water, etc., improve.

Describes the s inventions

Studies of the present invention were developed with the purpose of solving the above problems with respect to initial ideas about how to use sugars. First conducted a broad survey of changes in the intrinsic properties of compounds containing metal ions, allowing them to coexist with sugars in various combinations. In the result, it was revealed that the two are not regenerating sugar, trehalose and maltitol, expressed function to improve the properties of solubility of compounds containing metal ions, in particular increase their solubility in water and suppression of oxidative/reductive ability allowing them to coexist with compounds of metal ions, and that their function was significantly higher than that of other sugars. Then to study the mechanism of the above functions has conducted a detailed analysis on the molecular level interaction between trehalose or maltitol and compounds of metal ions. The survey revealed that trehalose and maltitol formed associates with compounds of metal ions and that the resulting composites showed above properties different from the properties of the intact metal compounds. According to the above results, it was found that the associates that can floor the raised opportunity to trehalose and maltitol co-exist with compounds of metal ions, demonstrated many advantages for their use in the food industry, compared with conventional preparations of compounds containing metal ions. In the case of components uterine salt solutions specific examples of such compounds containing metal ions, are compounds comprising compounds of magnesium ions and/or compounds of calcium ions, which are also formed and associates showed great dignity. The present invention was made on the basis described above, the original assumptions of the authors of the present invention. As a reference, in relation to associates of trehalose and salts, crystalline associates designed trehalose and calcium bromide in a molar ratio of 1:1, said William J. Cook et al. in "Carbohydrate Research, Vol. 31, pp. 265-275 (1973). Crystal associat was discovered in the course of their studies to clarify the mechanism of formation of dental plaque in the oral cavity. On the basis of their views, they did not offer industrial applications associate of trehalose and calcium bromide or its crystalline form. Therefore, the present invention discloses the associates of trehalose or maltitol and compounds of metal ions, with the exception of calcium bromide, or components uterine salt solutions, which can be used in various industries such as the industry, such as food processing, receipt and use.

As described above, the present invention solves the above problem by providing associates of trehalose or maltitol and or compounds containing metal ions, or components uterine salt solutions, and their production and use.

Brief description of drawings

Figure 1 shows the x-ray diffraction of crystalline associate of trehalose and calcium chloride (molar ratio 1:1).

Figure 2 shows x-ray diffraction of crystalline associate of trehalose and calcium chloride (molar ratio 1:2).

Figure 3 shows x-ray diffraction aqueous crystalline trehalose.

Figure 4 shows x-ray diffraction of the crystalline dihydrate of calcium chloride.

Figure 5 shows the absorption spectrum of infrared light, an associate of trehalose and calcium chloride (molar ratio 1:1).

Figure 6 shows the absorption spectrum of infrared light, an associate of trehalose and calcium chloride (molar ratio 1:2).

7 shows the absorption spectrum of infrared light by the associate of trehalose and magnesium chloride.

On Fig shows the absorption spectrum of infrared light, an associate of trehalose and strontium chloride.

Figure 9 shows the absorption spectrum of heate the CSOs light an associate of trehalose and chloride ferrous iron.

Figure 10 shows the absorption spectrum of infrared light, an associate of trehalose and the dichloride of copper.

Figure 11 shows the absorption spectrum of infrared light by the associate of trehalose and of Nickel chloride.

On Fig shows the absorption spectrum of infrared light, an associate of trehalose and manganese chloride.

On Fig shows the absorption spectrum of infrared light associates of maltitol and calcium chloride.

On Fig shows the absorption spectrum of infrared light associates of maltitol and chloride ferrous iron.

On Fig shows the absorption spectrum of infrared light powder that includes associat trehalose and component scale.

The best way of carrying out the invention

The present invention relates to associates of trehalose or maltitol and compounds containing metal ions (ion-metallic compounds), with the exception of calcium bromide or components uterine salt solutions, and to their preparation and use. Used in the present invention, the term "trehalose" means α,α-trehalose, a disaccharide in which two glucose molecules are linked together at their reducing groups by α,α-communication. Used in the present invention, the term "maltitol" means a sugar alcohol, which can be obtained by recovery of maltose, a disaccharide in which two is olekuly glucose linked by α -1,4-glucoside communication. In the present invention trehalose and maltitol are not restricted to their purity and form (liquid, amorphous powder, hydrated crystalline powder, anhydrous crystalline powder etc) as long as each of them forms associates with the following compounds of metal ions. Trehalose and maltitol, which can be used in the present invention, can be obtained in the usual way, but you can use a commercially available connection. For example, as commercially available trehalose can be used "TREHA®", powder aqueous crystalline trehalose food grade (trehalose content of 98% or higher on the analysis HPLC)supplied in the sale of the company Hayashibara Shoji, Inc., Okayama, Japan. "MABIT®"syrup food grade, including maltitol (74% or higher on a dry solid basis, the content of maltitol 75% or higher relative to the total sugar content by HPLC analysis) and "Crystal MABIT", an anhydrous crystalline maltitol food grade (the moisture content of 1.5% or lower, the content of maltitol 99% or higher by HPLC analysis) can be used as maltitol commercially available.

Used in the present invention, the term "compound containing metal ions" means a compound having a metal ion as a cation in the compounds is within the ionic bond between the cation and the anion, and including salt, alkali or complex. In the present invention can preferably be used any compounds containing metal ions, with the exception of calcium bromide, which can form associates with trehalose or maltitol. For example, compounds containing metal ions include one or more metal ions with monovalent (monovalent) or having a large valence ionic charges as a cation, in particular one or more ions of metals of groups 1 to 16 of the periodic table of elements, more specifically one or more metal ions selected from lithium, sodium, potassium, rubidium, etc., from group 1, beryllium, magnesium, calcium, strontium, etc. from group 2, scandium, yttrium, etc. from group 3, titanium, zirconium, hafnium, etc., from group 4, vanadium, niobium, tantalum, etc. from group 5, chromium, molybdenum, tungsten, etc. from group 6, manganese, technetium, rhenium, etc., from group 7, iron, ruthenium, etc. from a group 8, cobalt, rhodium, etc. from group 9, Nickel, palladium, etc. from a group of 10, copper, silver, etc. from group 11, zinc, etc. from group 12, aluminum, gallium, etc. from group 13, Germany, etc. from group 14, antimony, etc. from group 15 and polonium and so from group 16. In these compounds, compounds containing metal ions that includes one or more metal ions selected from the group of ions of alkaline-earth metals, that is their as calcium ions, magnesium, strontium, etc., ions of metals belonging to the transitional elements, such as ions of bivalent iron, copper, Nickel, manganese, zinc, etc. and ions of alkali metals such as sodium, potassium and so on, can be relatively successfully used as associates, as described in detail below. As metal compounds, including metal ions with divalent or more charges, more useful, especially can be used in the present invention. One or more anions selected from halogen ions such as fluorine ion, chlorine ion and bromine ion (except in the case of metal ions, having as counterion calcium ion), inorganic anions, such as sulfate ion, sulfite ion, ion monopersulfate, thiosulfate ion, carbonate ion, bicarbonate ion, nitrate ion, ion phosphate ion monohydrogenphosphate, ion dihydrophosphate, chlorite ion, hydroxide ion, ammonium ion, and organic anions such as acetate ion, lactate ion, citrate ion, fumaric ion and ion malate, can be used as anions for compounds containing metal ions, in the present invention. Because compounds containing metal ions with inorganic anions are relatively useful, as described below, mainly it is possible to use compounds containing metal ions, including the content of inorganic fillers anions. In relation to the use of compounds containing metal ions, the present invention in living organisms, it is desirable to use a physiologically acceptable compounds containing metal ions. Hereafter, the term "compounds containing metal ions" means all compounds containing metal ions, with the exception of calcium bromide.

Used in the present invention, the term "associates" means substances that are associated trehalose or maltitol and compounds containing metal ions through direct interaction. Such associates in the present invention is essentially constructed trehalose or maltitol and compounds of metal ions. Used in the present invention, the term "direct communication" means hydrogen bonding, the strength of van der Waals, ionic bond or a coordinate bond, and includes links in solid, gaseous, liquid or pasty state. Also used in the present invention, the term "essentially designed trehalose or maltitol and compounds of metal ions" means the associates, which usually essentially designed trehalose or maltitol and compounds of metal ions, and, depending on the circumstances, in addition, means compounds that include as ingredients other molecules, such as tie the fair water. Compounds containing metal ions in the above associates are usually associated with trehalose or maltitol in neutralized form (for example, salts, etc.) metal ion and its counterion. Depending on the circumstances of the metal ions may be associated with trehalose or maltitol, and the counterion bound to neutralize received associates. Associates of the present invention can be identified as follows: Associates formed in solution, can be identified by the method of nuclear magnetic resonance (NMR), for example described in "Jikken-Called-Kohza (Course of experimental chemistry) 5" edited by the Chemical society of Japan, published by Maruzen Co., Ltd., pp. 221-224 (1991). Associates can be identified by analysis of the solution, including associat of the present invention, and a solution that does not include associate, for example including only trehalose or maltitol, using NMR, comparing the relaxation time of the component atoms and identifying the phenomenon that atoms of associate are shorter relaxation times. Further, these associates can be identified by the fact that one or more signals associated with the chemical shifts (ppm)are signals with different chemical shifts, compared with the corresponding signals observed in the case of using only trehalose of maltitol. In addition, associates can also be identified by their crystallization in solutions, the selection of the obtained crystals and crystallographic analysis of their structure. In particular, the composites of the present invention can identify steps in their analysis by x-ray diffraction and comparison of radiographs with those in the case of crystalline trehalose, maltitol or crystalline compounds of metal ions, respectively, and confirm that the pattern of x-ray diffraction associates do not agree with any of those obtained from trehalose or maltitol and compounds of metal ions and their combined paintings. The above associates the present invention include compounds containing metal ions (or metal ions) in a molar ratio with trehalose or maltitol usually 0.5 or higher but not higher than 5, preferably 1 or higher, but not higher than 4. As in the case of crystalline associates of trehalose and calcium chloride, which are described in detail below in the "examples", associates can give a prescribed molar ratio of trehalose or maltitol connection with metal ions, such as about 1 or 2.

Associates of the present invention can be obtained by mixing trehalose or maltitol with compounds of metal ions. Mixing can be performed providing the contact trehalose or maltitol with compounds of metal ions. It is generally preferable to mix them in with the same solvent conditions under which dissolve both components. As a solvent, you can use water, ethanol, methanol, acetonitrile, dimethylsulfoxide, dimethylformamide and acetic acid. In case of receipt of associates for use in living organisms in the areas of food production, cosmetics and pharmaceuticals, it is desirable to use a physiologically acceptable solvents such as water and ethanol. In the case of aquatic forms trehalose can be used to form the associates of compounds containing metal ions, or compounds containing metal ions, with the inherent hygroscopicity, such as calcium chloride, mixing them into solid forms. Although a proportional relationship between trehalose or maltitol and compounds of metal ions depends on the type of compounds containing metal ions, the molar ratio between the compounds of metal ions and trehalose or maltitol can preferably be set in the range of usually 0.01 or above, but not more than 1000, preferably 0.1 or higher but not higher than 10. As in the case of associates of trehalose and calcium chloride, which are described in detail below in the "examples", associates can now be obtained by mixing trehalose or maltitol and with the joining, containing metal ions, in a prescribed molar ratio, such as about 1 or 2.

The associates of trehalose or maltitol and compounds containing metal ions, formed as described above can be used in intact form, for example in the form of solutions, and they can also be used in isolated form. For example, extraction, filtration, concentration, centrifugation, dialysis, precipitation, crystallization, hydrophobic chromatography, gel filtration chromatography and affinity chromatography can be used as a way to highlight associates.

Associates formed as described above, or containing fractions can be collected through methods such as crystallization, precipitation, concentration and drying (including spray drying, drying in vacuo and lyophilization). Depending on the types of compounds containing metal ions, the associates, which can be obtained by the methods described above have the following favorable properties compared to conventional preparations of compounds containing metal ions:

(1) Reduced hygroscopicity

The halides of alkaline-earth metals, including calcium chloride, have hygroscopic properties. Properties of hygroscopic compounds containing metal ions, significantly reduced the formation of associates with trega what osoi or multicolor. Therefore, the composites of the present invention, which include compounds containing metal ions having inherent hygroscopicity, characterized by the presence of characteristic features satisfactory manipulation. Such properties can with advantage be used to obtain funds, suppressing hygroscopicity. For example, it is possible to suppress the absorption of marine products, including components uterine salt solutions.

(2) Inhibition of the formation of sparingly soluble or insoluble compounds containing metal ions

In some cases, metal ions form salts with low solubility in water, depending on the type of counterions, as in the case of calcium phosphate. When the counterion which forms sparingly soluble or insoluble salts with metal ions, are added to a solution comprising such metal ions, rapidly formed and deposited substance (salt)having low solubility. The formation of such insoluble or insoluble salts is possible to suppress the formation of associates of compounds comprising such a metal ion and trehalose or maltitol, before the formation of sparingly soluble or insoluble salt, which is made water-soluble compound that includes a metal ion. Therefore associates compounds, including ion meta is La, which is inherent in the power of education sparingly soluble or insoluble salts, and trehalose or maltitol can be used as drugs, the deposition or formation of sediment in the water can be suppressed. Such properties can be used for suppressing deposition means for insoluble go insoluble salts.

(3) Improved solubility in water

Compounds containing metal ions, which are in the form associates with trehalose or maltitol, have in many cases a higher solubility in water than the solubility characteristic of compounds containing metal ions. For example, if the Association is improving the solubility in water of compounds of transition metal ions, such as salts of manganese, Smalley Nickel, iron salts, and salts of copper, calcium salts, magnesium salts and sodium. Therefore, trehalose and maltitol can preferably be used as an agent for improving the solubility of such compounds containing metal ions. In addition, associates of the above compounds containing metal ions, and trehalose or maltitol can mainly be used in industries of food, cosmetics and pharmaceutical preparations, if desired software solution, including compounds containing metal ions in the high concentrations.

As a result of extensive research of the authors of the present invention found that the above properties associates, as described in paragraphs (2) and (3), consisted in the formation of water soluble compounds such as chelates. It was also found that the associates could preferably be used for the following applications, because they had no toxicity, had a relatively high security and had a relatively low impact on the environment.

<1> As solubility in aqueous solutions of compounds containing metal ions, in particular compounds of calcium ions, it is possible to improve the education of associates with trehalose or maltitol, trehalose and maltitol can be used to prevent sedimentation and turbidity of drinks, including such compounds, such as soft drinks, isotonic drinks and perfusions with the addition of mineral substances.

<2> the Precipitation of sparingly soluble salts, in particular compounds of magnesium ions formed when cooking food with their boiling and dishes cooked in the container, it is possible to suppress the formation of associates with trehalose or maltitol. The result can largely suppress the formation of scale.

<3> the Precipitation of sparingly soluble salts, in particular marniemahaney fatty acids, you can suppress the formation of associates with trehalose or maltitol when soap is dissolved in hard water, including compounds containing metal ions. As a result, the formation of soap flakes can be significantly suppressed, and thus deteriorating almost foaming and cleaning ability of the soap solutions.

<4> the Precipitation of sparingly soluble salts, particularly iron salts and magnesium salts, which, by their inherent properties, formed when a store or boil hard water, it is possible to suppress the formation of associates with trehalose or maltitol. In the result, it is possible to suppress the increase in turbidity and the formation of scale.

<5> In the case of sparingly soluble or insoluble substances formed by the Association of compounds containing metal ions, organic substances, with the exception of sugars, such as glycosides, polyphenols, the solubility of organic substances can be improved by associating compounds containing metal ions, with trehalose or maltitol.

<6> Dirt, which is formed by compounds containing metal ions can be prevented and easily removed by washing with the formation of associates of compounds containing metal ions, and trehalose or maltitol. Therefore, trehalose and maltitol can preferably be used as the prevent is his agent, removing agent, washing agent and means for the bottom layer of the bath when the pollution compounds of metal ions. These agents can preferably be used to prevent or remove contamination on the surfaces of glass, metals, cars, houses, clothes and phone

<7> In the case of dental stones or dental deposits formed from compounds containing calcium ions, and compounds containing magnesium ions, their adhesion can be suppressed, and their dissolution can be facilitated by associating compounds containing metal ions, with trehalose or maltitol. Therefore, trehalose and maltitol can preferably be used for rinsing the oral cavity and tooth paste.

(4) Suppression of oxidation or reduction reactions

Ions of transition metals such as iron and copper and other metals can be oxidized or to recover, depending on conditions. It is possible that such reactions of oxidation and reduction spoil other substances, which may coexist with such ions. When such compounds containing metal ions, form associates with trehalose or maltitol, oxidative or reductive reactivity is usually suppressed. Therefore associates compounds containing metal ions, which have activity for the oxidation or reset the program, such as salts of iron and copper, and trehalose or maltitol can preferably be used as drugs compounds containing metal ions, without disturbing qualities of other substances. Properties such as oxidation and spoilage of the substances that are easy to oxidize or deteriorate in the joint presence of relatively small amounts of iron salts or copper salts, for example vitamins such as L-ascorbic acid (hereinafter may be briefly called "ascorbic acid") and Tocopherols, highly unsaturated fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DPC), odorants and colorants, it is possible to suppress mixing with trehalose or maltitol for education associates.

Powdered products, including associates of trehalose or maltitol and compounds containing metal ions, which include compounds of magnesium ions and/or compounds of calcium ions (which can be briefly called a "powder-like product, including associates"), you can get stages of mixing trehalose or maltitol with compounds of metal ions, which include compounds of magnesium ions and/or compounds of calcium ions, educational associates, and then grinding associates in the powder. It is preferable to mix trehalose or maltitol connection (the connection is s) of metal ions, including the connection of magnesium ions and/or connection of calcium ions, which are dissolved in an aqueous solvent, for education associates. The molar ratio between the compound (compounds), metal ions, including the connection of magnesium ions and/or connection of calcium ions, and trehalose or maltitol preferably set in the range of usually 0.001 to 10, preferably 0.01 to 5. If the molar ratio is lower than 0,001, it is inconvenient to use the mixture as an agent, rich in minerals, or similar, due to the low content of associates, including the connection of magnesium ions and/or connection of calcium ions. If the molar ratio is higher than 10, the hygroscopic powdered products, including associate, which is produced using a mixture improves enough.

Compounds containing metal ions, comprising compounds of magnesium ions and/or compounds of calcium ions, can be obtained by mixing commercially available compounds of magnesium ions and compounds of calcium ions in the proper proportions. Uterine saline is an example that includes both compounds in a mixed form. In such uterine saline solution can optionally add other compounds containing metal ions and organic substances such as sugars and related to kaharian substances.

Uterine saline solution is usually prepared by the steps of concentration of sea water by way of heating and/or by way of exchange of ions, precipitation of sodium chloride, separating the obtained sodium chloride and collecting the residual solution. Uterine saline solution has a strong, pungent odor and unpleasant taste such as bitter taste, and includes compounds of magnesium as a main component and connection of calcium ions, compounds of potassium ions and compounds of sodium ions as other components. Uterine saline was used as material for the production of salts of magnesium and potassium, which improves the flavor agents for soy milk, and coagulating agents for "tofu" cheese from soy beans).

Components uterine saline mean compounds containing metal ions, including saline solution, and comprising at least compound of magnesium ions and/or compounds of calcium ions. Usually uterine saline sold in liquid form and includes as components, for example, of 17.5% (wt./wt.) of magnesium chloride and 8.1% (wt./wt.) of calcium chloride, 3,6% (wt./wt.) potassium chloride, 2.9 percent (wt./wt.) sodium chloride. Dried salt powders, which can be obtained by concentration of the intact uterine salt solution followed by crystallization or drying and grinding into powder, have relatively strong hygroscopicity and quickly turn into a liquid form due to their hygroscopicity during storage in high humidity.

Associates, formed by the method of the present invention, can be obtained in powder form (in addition to liquid and paste forms) through methods such as crystallization, fractional precipitation, concentration and drying (including spray drying, drying in vacuo and lyophilization). Undesirable properties, such as property, hygroscopicity, healing ability, the oxidizing capacity and low solubility in water at industrial operations with conventional powdered preparation of compounds containing metal ions, comprising compounds of magnesium ions and/or compounds of calcium ions, improve in powdered products, including thus obtained associates. Therefore, powder products, including associates, have satisfactory characteristics.

Associates and containing powdered products of the present invention, which are described above, very useful in a variety of areas in which the use of compounds containing metal ions or uterine saline as materials, ingredients and products, for example food products (including beverages), agricultural and marine products, cosmetic products, pharmaceuticals, commodities, products of chemical industry and production is the rotary materials and ingredients, used in these areas. They can be used in isolated form or optionally in form of the composition along with other ingredients, for example one or more excipients and carriers such as calcium carbonate, calcium phosphate, lactose, sugar alcohols, cyclic saccharides, dextrin, starch and cellulose. It is desirable that other ingredients that can be used along with associates or powdered products, including associates of the present invention, were biologically acceptable ingredients in case of using such compositions in living organisms. In the case of use in the food industry associates and containing powdered products of the present invention can be used in addition, for example, sweeteners such as sucrose, glucose, maltose, L-fucose, L-rhamnose, stevia, Glycyrrhiza glabia, methyl ester with L-aspartyl L-phenylalanine, glycyrrhizinate and Sucralose, acidifying agents, such as adipic acid, citric acid, glucono-Delta-lactone, acetic acid, tartaric acid, fumaric acid, lactic acid, spices, such as sodium aspartate, alanine, citric acid, glutamic acid, theanine and sodium chloride, one or more coloring agents, flavors, flavor enhancers, causing the swelling agents, preservatives, leavening agents, agents against oxidation, decolorizing agents, agents, giving pasty consistency, stabilizing agents and emulsifiers, which are usually used in food products.

Associates and containing powdered products of the present invention can also be used for food; specific examples are various condiments, such as table salt, soy sauce, powdered soy sauce, "miso" (bean paste), "formats-miso" (a powdered "miso"), "moromi" (refined sake), "Helio" (a refined soy sauce), "furikake" (fish dish with seasoning), mayonnaise, dressing, vinegar, "sanbai-zu" (a sauce of sugar, soy sauce and vinegar), "formats-sushi-zu" (powdered vinegar for sushi), "Chuka-no-Moto" (an instant mix for Chinese dishes), "tentsuyu" (a sauce for Japanese deep fried in excess fat food), "mentsuyu" (a sauce for Japanese vermicelli), broth, sauce, ketchup, "yakiniku-no-tare" (a sauce for Japanese meat, roasted on the grill), spicy peppery food seasoning, instant formula for stewed foods, instant soup mix, "Dashi-no-Moto" (an instant stock mix), seasoning of nucleic acids, mixed seasoning, "mirin" (sweet biscuits), "Shin-mirin" (a synthetic mirin), talovy sugar and coffee sugar; various "wagashi" (Japanese biscuits), such as "senbei" (a rice cracker), "Arara" (rice cakes in the form of cubes), "okoshi" (prosobee and rice biscuits), "urine" (a rice paste) and the like, "Magno" (sweet bun with a bean-jam), "uiro" (a sweet rice jelly), "Ann" (a bean jam) and the like, "yokan" (a sweet jelly of beans), "wwe-yokan" (soft jelly from the beans azuki), "bungaku" (Jacana), jelly, PAO de Castella, and "amedama" (Japanese candy); Western confectioneries such as sweet bread with raisins, biscuit, cracker, cookie, pie, pudding, butter cream, egg and milk cream, puff pastry with cream, waffle, sponge cake, doughnut, chocolate, chewing gum, caramel and candy; frozen desserts such as ice cream and sherbet; syrups such as "kajitsu-but-syrup-zuke" (preserved fruit) and "koremitsu" (a sugar syrup for DescribeMacro ice); pastes such as flour paste, peanut paste, fruit paste and spread products; processed fruits and vegetables such as jam, marmalade, "syrup-zuke" (fruit pickles), and "current" (canned); pickles and salted or pickled products such as "fukujin-zuke" (pickles from red radish), "bettara-zuke" (a kind of pickles from solid red radish), "senmai-zuke" (kind of sliced layers of pickles from fresh radish) and "rakkyo-zuke" (marinovanni is or pickled shallot); the premixes for pickles and pickled or salted products such as "takuan-zuke-no-Moto" (a premix for pickled or salted radish) and "hakusai-zuke-no-Moto" (a premix for pickles fresh white cabbage); meat products such as ham and sausage; products of fish, such as fish ham, fish sausage, "kamaboko" (steamed fish paste), "chikuwa" (a kind of fish paste) and "tempura" (Japanese fried in excess fat fish pasta); dried seafood such as dried seaweed, dried whole fish, gutted and dried fish; "chinmi" (relish)such as "uni" (sea urchin), "IKA-no-shiokara" (salted guts of squid), "su-konbu" (processed seaweed), "Saki-surume" (dried strips of squid), "fugu-no-mirin-boshi" (a dried seasoned with mirin dog fish); "tsukudani" (foods boiled in soy sauce)such as food from the red seaweed, edible wild plants, dried squid, small fish and aquatic animals that have a shell; daily dishes such as "nimame" (cooked beans), potato salad and "konbu-Maki" (rolled seaweed); dairy products such as yogurt and cheese; canned in banks and flooded bottled products such as meat fish, meat animal, fruit and PSBs and; alcoholic beverages such as sake, synthetic sake, liqueur and Western liquors; soft drinks such as coffee, tea, cocoa, juice, isotonic drinks, drinks with mineral supplements, drinks, rich in minerals, carbonated beverages, beverages based on fruit juices, yogurt drinks, drinks containing lactic acid bacteria, vegetable juice, soy milk, food of fast preparation, such as a mixture for quick cooking pudding mixture for quick hot cake, "sokuseki-shiruko" (mix for quick cooking soup beans azuki with rice cake), and instant soup mix; solid foods for babies, medical foods; health drinks such as ginseng extract, leaf extract, bamboo extract, plum extract, pine needles, turtle extract, Chlorella extract, aloe extract, propolis and other foods and drinks, such as peptide foods, frozen foods, health foods, viable cells of lactic acid bacteria and yeast, and Royal jelly; mineral enriching substances, including calcium and/or magnesium, growth-promoting agent for Bacillus natto, agent, improves the taste, the agent that improves wcastle soy milk and coagulating agent for the production of "tofu" cheese from soy beans).

In the case of associates and powder including products of the present invention in the field of agricultural and marine products can preferably be used intact or in the form of a composition that includes other additional ingredients for the food and pet food or nutritional supplements, or activating agents for plants. As other ingredients that can be added to the composition, you can use one or more of the ingredients that are in General use in the following relevant areas, for example food or foodstuff ingredients, such as squeezed sugar cane, the core of the corn cob, rice straw, hay, grain, wheat flour, starch, fat, flour, sugar waste, wheat bran, legumes, bread, different kinds of breads prepared using fermentation, chips, sheets and the like, the ingredients for nutritional supplements, such as nitrate, ammonium salts, urea, phosphate and potassium salts.

They can also preferably be used in various materials for concentrated food, food mixes and blends for animals, such as poultry, honey bees, silkworms, insects and fish, and nutritional supplements and activating agents for plants,such as cultivated plants, such as cereals and potatoes and the like, vegetables, tea bushes, fruit trees, garden plants, roadside trees and grass on the Golf course.

In the case of use in the present invention associates and including their powdered products in the form of compositions in the fields of cosmetics and pharmaceuticals usually you can use one or more of the following ingredients traditionally used in these areas, such as agents, moisture, detergents, dyes, flavorings, enzymes, hormones, vitamins, agents that absorb ultraviolet (UV) rays, agents, UV shielding, solvents, stabilizers, plasticizers, softening agents, solubilization, reducing agents, buffers, sweeteners, Foundation, agents that promote evaporation, adsorbents, the corrigentov, synergists, binders, suspendresume agents, antioxidant agents, brighteners, covering agents, humidifiers, cooling agents, emollients, emulsifiers, excipients, antiseptics and preservatives.

They can also preferably be used for specific products, such as cosmetics such as milky lotion, cream, shampoo, conditioner, means for processing the skin, lipstick, blush, going to the lip, lotion, means for baths and toothpaste; products used to obtain pleasurable sensations, such as tobacco and cigarettes; pharmaceutical preparations, such as liquid pharmaceutical preparation for oral administration, tablet, pill, toffee, butter, cod liver oil in the form of droplets, means for refreshing the mouth, capsule, solution for rinsing the mouth, magnesium Supplement and a means for enrichment of minerals; and stabilizing agents for various enzymes.

To obtain the above-described compositions, it is preferable to add associates or including their powdered products in the composition in the range of usually 0,00001-75% (wt./wt.), preferably of 0.0001 to 50% (wt./wt.), it is advisable to 0.001-25% (wt./wt.), based on the dry solid basis (STO).

Because trehalose and maltitol inhibit the formation of scale during cooking of meat and vegetables, soap suds and cereals, they can preferably be used as the agent, suppressing the formation of scale, soap suds and flakes for seasoning subjected to boiling products and dishes, served in a bowl, mineral water, means for baths and Soaps.

Next, trehalose inhibits the elution of compounds of magnesium ions from food materials such as meat and vegetables while cooking. Therefore, trehalose can preferably be used as the vast suirou is the agent for compounds of magnesium ions for seasonings subjected to boiling of foods and dishes, brewed in a pot. Elution of compounds of magnesium ions, which constitute the mineral components of food materials is suppressed by using these spices. So the seasoning can be used for holding nutrients and to preserve the taste of food materials.

The following experiments 1-3 explain what trehalose and maltitol form associates with various compounds, metal ions and components uterine fluids. Experiments 4-8 explain the use of associates. Next, experiments 9-12 explain the inhibitory effect of trehalose and maltitol on the formation of scale during cooking and the overwhelming effect of trehalose on the elution of compounds of magnesium ions.

Experiment 1

Associates formed from trehalose and calcium chloride

Experiment 1-1

Crystal associat formed from trehalose and calcium chloride

Experiment 1-1(a)

The selection of crystalline associate

147 grams (one mol) of the dihydrochloride of calcium were placed in 1-liter chemical beaker was mixed with 250 g of deionized water and completely dissolved by heating. In the conditions of constant heat to the solution was added 378 grams (one mol) of crystalline trehalose dihydrate, simply referred to as "water is kristallicheskaja trehalose", and completely dissolved. After stopping the heating and separation of chemical beaker at room temperature (about 25°C) for 2 days at the bottom of the chemical glass of observed sediment, including crystals. The crystal was transferred into a centrifugal separator of the type buckets and washed by spraying it appropriate quantity of water and collecting the resulting crystals. The collected crystals were dried in vacuum at 40°C for 4 h Then the crystals were thoroughly dried in a desiccator containing phosphorus pentoxide at room temperature for 20 hours resulted In approximately 200 g of white crystalline powder.

294 grams (two moles) of the dihydrochloride of calcium were placed in 1-liter chemical beaker was mixed with 200 g of deionized water and completely dissolved by heating. In the conditions of constant heat to the solution was added 378 grams (one mol) aqueous crystalline trehalose, completely dissolved and was constantly heated. After boiling for about 30 min, observed the formation of crystals. After stopping the heating and separation of chemical glass at 60°C for 24 h, the contents of chemical glass was formed into a block, including crystals and the sugar solution. After removal of the block from chemical glass and rough cleaving the crystals were collected after premiani the spray appropriate amount of water using a centrifugal separator of the type buckets. The collected crystals were dried in vacuum at 40°C for 4 h and then thoroughly dried in a desiccator containing phosphorus pentoxide at room temperature for 20 hours resulted In approximately 400 g of white crystalline powder.

Experiment 1-1(b)

Physico-chemical properties of crystalline associate

(1)Analysis of x-ray diffraction

2 kinds of crystals obtained in two different ways, described in experiment 1-1(a), respectively, were analyzed to obtain the diffraction pattern of a conventional analysis of x-ray diffraction on the powder using x-ray diffractometer, "RAD-2B"sold by Rigaku Corporation, Tokyo, Japan. Diffraction of x-rays aqueous crystalline trehalose and dihydrochloride calcium also received the same way. The diffraction pattern of a crystal obtained from a mixture of trehalose and calcium chloride with a molar ratio of 1:1, which is obtained from a mixture of trehalose and calcium chloride with a molar ratio of 1:2, water crystalline trehalose dihydrochloride and calcium, all of which were obtained in experiment 1-1(a), shown respectively in figures 1-4. As is evident from the results shown in figure 1-4, x-ray diffraction figure 1 shows the main features of the RNA the diffraction angle (2θ ) 9,02°and other corners 17,98° and 21,90°, while x-ray diffraction figure 2 shows the main diffraction angle 12,66°, 21,02° and 25,48°. Both x-ray diffraction was completely different from those in the case of aqueous crystalline trehalose (figure 3) and calcium chloride (figure 4). These results indicate that the 2 kinds of crystals obtained in experiment 1-1(a), does not represent a mixture of water crystals crystalline trehalose and dihydrochloride calcium, and the crystals are completely different from crystals having independent crystal structure.

(2)Analysis components

Each crystal obtained in two different ways, were analyzed to determine the components as follows:

Trehalose

25 mg each of the above crystals was dissolved in 5 ml of pyridine containing 2 mg/ml phenyl-β-D-glucoside as an internal standard for gas chromatography. After the conversion of saccharide in 250 μl portions of each solution in trimethylsilane derived in the usual way, the sample was analyzed by gas chromatography (column "OV-17", sold by GL Sciences, Inc., Tokyo, Japan). Separately, aqueous crystalline trehalose as a standard, accurately weighed and analyzed by gas chromatography. The amount of trehalose per 1 g of the crystal in which each sample was calculated based on peak areas of the sample and standard.

Calcium

25 mg each of the above crystals was dissolved in 1% (vol./about.) chloride-hydrogen acid and the resulting solution was 100-fold diluted with 10% (wt./about.) a solution of lanthanum chloride. Then the calcium content in each diluted solution was measured using atomic absorption photometry (model 5100", sold by Perkin-Elmer Japan Co., Ltd., Yokohama, Japan). The amount of calcium chloride per 1 g of the crystal was calculated on the basis of the hypothesis that all of the calcium contained in the crystal is in the form of calcium chloride.

Moisture

The amount of moisture per 1 g of each of the above crystals was measured in the usual way loss after drying using 5 g of crystals.

The results obtained from the above analyses, summarized in table 1.

1,29

0,00
Table 1
Crystal*Quantity (mg)/g crystalThe molar ratio in the crystal
TrehaloseCaCl2**MoistureTrehaloseCaCl2**Moisture
1:1

1:2
704,6

604,5
276,7

399,0
48,0

0,0
1

1
1,21

2,03
* Refer to "1:1", "1:2" means the crystals obtained from solutions having molar ratios of trehalose and calcium chloride, respectively, 1:1 and 1:2.

** The amount of calcium chloride per 1 g of the crystal, calculated on the basis of the hypothesis that all of the calcium, determined by atomic absorption photometry, is in the form of calcium chloride.

Based on the results presented in table 1, it was found that the first crystal obtained in experiment 1-1(a), contained as ingredients trehalose, calcium chloride and water in the form of a crystalline monohydrate associate with the above ingredients, in a molar ratio of 1:1:1, and the second crystal contained as ingredients trehalose and calcium chloride in the form of an anhydrous crystal of associate with the above ingredients, in a molar ratio of 1:2. Here the first and second crystals obtained in experiment 1-1(a), called respectively "associat trehalose and calcium chloride (molar ratio 1:1)and associat trehalose and calcium chloride (molar ratio 1:2)".

Experiment 1-2

NMR analysis associates formed from trehalose and calcium chloride

For analysis of the mechanism of the Association of trehalose and calcium chloride in the two views of the x associates of trehalose and calcium chloride (molar ratio 1:1 and 1:2), conducted the following NMR analysis:

(1)With13NMR

50 mg two types of crystals associates of trehalose and calcium chloride (molar ratio 1:1 and 1:2) and water crystalline trehalose was dissolved in 1 ml of 99.9% deuterium oxide and analyzed for the13NMR as follows. NMR analysis was performed using a device model JNM-AL300"sold by JOEL Ltd., Tokyo, Japan, the dimension of the kernel With13and the resonance frequency 75,45 MHz. After installing the tubes containing the above solution, the instrument measured the time of spin-lattice relaxation (hereinafter called simply "relaxation time") of individual carbon atoms of trehalose in the solution in accordance with the method of recovery of inversion, described in the literature accompanying the appliance manual. A separate peak (chemical shift, ppm), obtained in the result of the analysis, attributed to specific atoms on the basis of the data described J.H. Bradbery et al. Carbohydrate Research, Vol. 126, 125-126 (1984). The classification of carbon atoms and the amount of time their relaxation are shown in table 2. (Data on one trehalose, associate of trehalose and calcium chloride (molar ratio 1:1) and the associate of trehalose and calcium chloride (molar ratio 1:2) are presented respectively in tables 2-1, 2-2 and 2-3.)

As shown in table 2, the relaxation time of the atom(ATO whom s) of carbon in the regulations(regulations) C2 and/or C4 decreased significantly in two species of associates of trehalose and calcium chloride (molar ratio 1:1 and 1:2). Therefore, it was assumed that the Association of trehalose and calcium chloride was direct interaction between the hydroxyl group associated with the atom (atoms) of carbon in the regulations (regulations) C2 and/or C4.

(2)N1NMR

50 mg two types of crystals associates of trehalose and calcium chloride (molar ratio 1:1 and 1:2) and water crystalline trehalose was dissolved in 1 ml of DMSO-d4 and analyzed using N1NMR as follows. NMR analysis was performed with the use of device "model JNM-AL300"sold by JOEL Ltd., Tokyo, Japan, the dimension of the kernel of H1, the resonance frequency 300,4 MHz and 8 units of time integration. A separate peak (chemical shift, ppm), obtained in the result of the analysis, attributed to specific atoms in accordance with two-dimensional NMR method described in "Jikken-Called-Kohza (Course of experimental chemistry)" PP. 302-312, edited by the Chemical society of Japan, published by Maruzen Co., Ltd., (1991) with reference to the results With13NMR in table 2. Chemical shifts of protons trehalose referred to the appropriate position in the molecule trehalose analysis, compared with shifts associates of trehalose and calcium chloride (molar ratio 1:1 and 1:2). Chemical shifts of protons, showing significant difference between trehalose and associates, summarized in table 3.

Table 3Assignment*Chemical shift (ppm)Tre**Tre-CaCl2(1:1)**Tre-CaCl2(1:2)**OH-2 or HE-44,808

4,804

4,791

4,7914,932

4,915

4,887

4,8754,850

4,833

4,827

4,811HE-34,625

4,6244,724

4,7054,657

4,637HE-64,419

4,400

4,3814,498

4,478

4,4594,430

4,410

4,391* The position of the hydroxyl group represented using numbers associated carbon.

** The symbol "Tre", "Tre-CaCl2(1:1)" and "Tre-CaCl2(1:2)," mean respectively trehalose, associate trehalose and calcium chloride (molar ratio 1:1) and associat trehalose and calcium chloride (molar ratio 1:2), respectively

As shown in table 3, the chemical shifts of all hydroxyl protons trehalose significantly changed at the Association of trehalose with calcium chloride, in comparison with the chemical shifts of intact trehalose. This result directly shows that trehalose and calcium chloride form associat interaction hydro is a strong proton trehalose with calcium chloride. When considering the above analysis result With13NMR suggested that the interaction of the hydroxyl proton (proton)associated with the atom (atoms) of carbon in the regulations (regulations) C2 and/or C4, and calcium chloride were largely involved in the formation of two kinds of associates of trehalose and calcium chloride (molar ratio 1:1 and 1:2).

Experiment 2

Associates formed from trehalose and other compounds containing metal ions or components uterine solutions

Experiment 2-1

NMR analysis associates formed from trehalose and magnesium chloride, strontium chloride or components uterine saline

A mixture composed of 20,3 g hexahydroborite magnesium and 37.8 g of water crystalline trehalose (molar ratio 1:1), was mixed with 20 g of deionized water and completely dissolved by heating. Similarly, a mixture composed of 26,6 g hexahydroborite strontium and 37.8 g of water crystalline trehalose (molar ratio 1:1), was mixed with 20 g of deionized water and completely dissolved by heating. After cooling to ambient temperature above solutions were dried in vacuum at 80°C for 15 p.m Obtained dried material appropriately crushed to obtain two kinds of powder.

20 ml (containing 6.42 per g dry solid matter) of commercially available components uterine saline sold by Sanuki Engyou Co., Ltd., Kagawa, Japan, was mixed with 10 g of water crystalline trehalose and completely dissolved with heating. The solution was dried in vacuum at 80°C for 15 h, and the resulting dried material appropriately crushed to obtain powder.

(1)With13NMR

In accordance with the method described in experiment 1-2, 50 mg of any of the above three kinds of powders, respectively, was dissolved in 1 ml of deuterium oxide and using13NMR analyzed the magnitude of the relaxation time of each of the carbon atoms of trehalose. Received such a way relative values of the relaxation time of each of the carbon atoms, relative to the values obtained for only trehalose, was calculated on the basis of the results of water crystalline trehalose obtained in experiment 1-2 table 2-1. The results are summarized in table 4.

Table 4
1.Associat trehalose and magnesium chloride
Assignment (No. of carbon atom)Chemical shift (ppm)The relaxation time
(MS) (%)*
1

2

3

4

5

6
95,917

75,213

74,843

73,730

72,395

63,230
392,3

462,3

392,0

467,8

418,6

274,6
96

104

92

103

84< / br>
101
*: The relative relaxation time when the relaxation time of trehalose is taken for 100%. Bold font indicates that the relaxation time of the carbon atom is significantly decreased by the Association.
2.Associat trehalose and strontium chloride
Assignment (No. of carbon atom)Chemical shift (ppm)The relaxation time
(MS)(%)*
1

2

3

4

5

6
95,834

75,139

74,760

73,648

72,312

63,172
377,4

422,3

345,5

415,7

398,3

288,6
93

95

81< / br>
91

80< / br>
107
*: The relative relaxation time when the relaxation time of trehalose is taken for 100%. Bold font indicates that the relaxation time of the carbon atom is significantly decreased by the Association.
3.Associat trehalose and components uterine salt rastv the RA
Assignment (No. of carbon atom)Chemical shift (ppm)The relaxation time
(MS)(%)*
1

2

3

4

5

6
95,886

75,183

74,813

73,700

72,365

63,200
406,8

484,8

410,5

486,1

424,4

273,6
100

109

96

107

85< / br>
101
*: The relative relaxation time when the relaxation time of trehalose is taken for 100%. Bold font indicates that the relaxation time of the carbon atom is significantly decreased by the Association.

As is evident from the results presented in table 4, all the powders obtained from a mixture of trehalose and magnesium chloride, powders of trehalose and strontium chloride and powders of trehalose and components uterine salt solution by dissolving and drying in vacuum, showed significantly reduced the relaxation time of the carbon atom in position, compared to the case with one of trehalose. From the obtained results it was found that trehalose formed associat with magnesium chloride, strontium chloride and components uterine saline direct interaction, as in the case of calcium chloride, i.e. the above three kinds of powder p is estableci respectively associates of trehalose and magnesium chloride, trehalose and strontium chloride and trehalose and components uterine saline. Further, from the results shown in tables 4-1, 4-2 and 4-3, it was assumed that the interaction of the hydroxyl group (s)associated with the atom (atoms) of carbon in the regulations (regulations)-2 and/or C-4 trehalose and compounds containing metal ions, deeply involved in the formation of associate of trehalose and magnesium chloride, trehalose and strontium chloride and trehalose and components uterine saline.

(2)N1NMR

In accordance with the method of experiment 1-2, 50 mg of any of the above three kinds of powders, respectively, was dissolved in 1 ml of DMSO-d4 and analyzed using N1NMR. Spent the correlation of proton peaks (chemical shifts, ppm) trehalose observed when N1NMR, to specific carbon atoms. The results are presented in table 5.

As in the case of associates of trehalose and calcium chloride chemical shifts of hydroxyl protons associates of trehalose and compounds containing metal ions, was significantly different from the chemical shifts of a trehalose. The chemical shifts of these protons are presented in table 5 along with shifts one trehalose, shown in table 3.

The table is 5
Assignment*1Chemical shift (ppm)
Tre*2Tre-MgCl2*2Tre-SrCl2*2The components of the triple salt solution*2
HE-2 or HE-44,808

4,804

4,791

4,791
4,985

4,970

4,867

4,761
4,932*34,985

4,970

4,867

4,761
HE-34,645

4,624
4,661

4,643
4,738

4,720
4,661

4,643
HE-64,419

4,400

4,381
4,514

4,495

4,475
4,487*34,514

4,495

4,475
*1 the Position of the hydroxyl group represented using numbers associated carbon atom.

*2 Symbol "Tre", "Tre-MgCl2", "Tre-SrCl2and Tre-components uterine saline" mean respectively associat trehalose and magnesium chloride, associate trehalose and strontium chloride and powder product, including associate trehalose and components uterine saline.

*3 Large letter means that multiple peaks (signals)observed in the case of trehalose, were grouped in one position.

As shown in table 5, all chemical shifts hidroxil the x proton trehalose significantly changed, when associates was formed from trehalose and magnesium chloride, strontium chloride or components uterine saline, compared to the case with one of trehalose. The results directly show that trehalose is also formed complexes with magnesium chloride, strontium chloride and components uterine saline. Taking into account the above results With13NMR suggested that the interaction of the hydroxyl proton (proton)associated with the atom (atoms) of carbon in the regulations (regulations)-2 and/or C-4 trehalose and compounds containing metal ions, was particularly deeply involved in the formation of the above associates of trehalose and compounds containing metal ions.

Experiment 2-2

Change the solubility of compounds containing metal ions, Association with trehalose

Change the water solubility of trehalose and/or compounds containing metal ions, was investigated in terms of joint presence of trehalose and compounds containing metal ions. For the test data used water crystalline trehalose. Hexachloride strontium, dichlorhydrate monovalent copper, tetrachloride ferrous iron, tetrachloride manganese and hexachloride Nickel was used as compounds containing metal ions, d is I test data. of 37.8 g of water crystalline trehalose (0.1 mol) and 0.1 mol of any of the above compounds containing metal ions, were placed in a 100 ml glass chemical beaker and mixed with deionized water to obtain 30 g of water chemical glass with account bound water trehalose and compounds containing metal ions, and then chemical content of the glasses was dissolved with heating. Solutions obtained by dissolving the same amount of one of trehalose, or solutions of some compounds containing metal ions, were prepared as controls. After the complete dissolution of all the chemical content of the glasses was allowed to stand at room temperature (25° (C) within 24 hours Then about the deposition of crystals was judged using microscopic observations. In the case of the deposition of crystals formed crystals were collected and analyzed to identify the specified component (components) in the usual way. The results are presented in table 6.

Table 6
The compound containing metal ionsThe deposition of crystals
In the presence of trehaloseIn the absence of trehalose
Strontium chlorideCrystalline chlorite is strontium slightly deposited. Precipitation (as strontium chloride)
Chloride monovalent copperCrystalline chloride monovalent copper is slightly deposited.Precipitation (chloride monovalent copper)
Chloride ferrous ironCrystalline chloride ferrous iron was slightly deposited.Precipitation (chloride ferrous iron)
The chloride of manganeseNoPrecipitation (manganese chloride)
Chloride NickelNoPrecipitation (Nickel chloride)
None (control)The precipitation of crystalline trehalose---

Turning to the case of strontium chloride, which was confirmed by NMR analysis (experiment 2-1), forms associate with trehalose crystals of strontium chloride is clearly observed in the absence of trehalose. However, the number of crystals was significantly decreased in the presence of trehalose. The result indicates that the Association of compounds containing metal ions, and trehalose can be judged by the study of the change of the solubility in water of compounds containing metal ions, in the presence of trehalose. Turning to the results of the study of chloride simultaneity the Lenten copper, chloride ferrous iron and of Nickel chloride (table 6, with the number of chloride monovalent copper on a number of Nickel chloride), water solubility of these compounds containing metal ions, has significantly improved in the presence of trehalose. Accordingly, based on the above judgments, it was found that these compounds containing metal ions, also formed associates with trehalose in the presence of trehalose. Compared with the results of the control (table 6, the lowest number)that does not include compounds containing metal ions, trehalose was deposited from a solution containing one trehalose, in the existing conditions of the experiment, while trehalose were not deposited in the presence of the above compounds containing metal ions. On the basis of the result it was found that the solubility inherent trehalose, as well as compounds containing metal ions used in this experiment have improved in the Association.

Experiment 3

Associate of maltitol and compounds containing metal ions

A mixture of 100 mg of maltitol (STO) and 85.3 mg of calcium chloride (STO) was dissolved in 1 ml of deuterium oxide. The solution was analyzed in accordance with13NMR described in experiment 1-2, and analyzed the relaxation time of each carbon atom politically peak (chemical shift, ppm)observed in this analysis, correlated to a specific carbon atom on the basis of the data described J.H.Bradbery et al. in Carbohydrate Research, vol.126, 125-126 (1984). The solution is prepared with 100 mg of maltitol (STO), were analyzed in a similar manner as a control. The results are summarized in table 7.

As shown in table 7, in the solution mixture of maltitol and calcium chloride values of the relaxation time of the carbon atoms in position C-4 of glucose balance in positions C-3' and C-5' residue of sorbitol were significantly reduced compared with the case of one maltitol. On the basis of the obtained results revealed that maltitol formed associate with calcium chloride by direct interaction. Further, from the above results suggested that the direct interaction of hydroxyl groups associated with the carbon atoms in position C-4 of glucose balance in positions C-3' and C-5' residue of sorbitol, and calcium chloride was mainly deeply involved in the formation of associates of maltitol and compounds containing metal ions.

Experiment 4

The hygroscopicity of associate of trehalose and calcium chloride or components uterine saline

For comparison of the hygroscopic properties bleed on the humid air) powder, including associat t is egality and calcium chloride, or associate trehalose and components uterine saline, control dried powder of calcium chloride or uterine saline, which, as you know, has the property of high hygroscopicity, conducted the following tests hygroscopicity. As the test samples used two types of crystalline associates of trehalose and calcium chloride (molar ratio 1:1 or 1:2)obtained in accordance with the method described in experiment 1-1 or 1-2, and powders included associates of trehalose and components uterine saline, dichlorhydrate calcium or powder of dried components uterine saline. The dried powder components uterine salt solution was obtained by drying the fallopian salt solution in vacuum at 60°C for 15 h, grinding into a powder and dried in the usual manner for use as a control. The moisture content (mass of moisture per 1 g of the sample) in each sample was measured in the usual way of determining loss during drying. The moisture content in the associate of trehalose and calcium chloride (molar ratio 1:1)associate of trehalose and calcium chloride (molar ratio 1:2), in the form of powder, including associate trehalose and components uterine saline, dichlorhydrate calcium or dried components uterine saline races who the thief was, respectively 0,048 g, 0,000 g 0,061 g, 0,245 g and 0,214, Data samples of approximately 1.5 g each, respectively, were placed in an aluminum Cup and kept at 25°C for 7 days in a desiccator, which was regulated to maintain a relative humidity of 33.0%, or 52.8 per cent. A lot of the content in each Cup was measured at the beginning of storage (day 0 of storage) and after 1, 2, 4 and 7 days after the beginning of storage. The amount of moisture per 1 g of each sample after 1-7 days of storage was calculated based on the assumption that increased the number of measured relative to the 0 day of storage, represented the amount of moisture absorbed by each sample. The results are presented in table 8.

0,010
Table 8
Relative humiditySample*The moisture content of sample (g)
0 day1 dayday 23 day3 day
33,0%CaCl2·2H2O0,2450,2890,3120,3430,364
Tre-CaCl2(1:1)0,0480,0480,0480,0480,048
Tre-CaCl2(1:2)0,0000,0100,0110,011
DPBC**0,2140,2990,3490,3780,398
Tre-BC***0,0610,164of 0.182of 0.1820,175
52,8%CaCl2·2H2O0,2450,3190,3590,4050,430
Tre-CaCl2(1:1)0,0480,0540,0590,059to 0.060
Tre-CaCl2(1:2)0,0000,0160,0170,0180,021
DPBC**0,2140,4120,4620,4950,523
Tre-BC***0,0610,2530,294of 0.3370,356
* Designate "CaCl2·2H2O", "Tre-CaCl2(1:1)", "Tre-CaCl2(1:2)" and "Tre-BC" means the dihydrochloride of calcium, associate trehalose and calcium chloride (molar ratio 1:1), associat trehalose and calcium chloride (molar ratio 1:2) and powdered product, including associate trehalose and components uterine saline.

** Dried powdered components of the fallopian salawag the solution.

*** The components of the uterine saline.

As shown in table 8, the dihydrochloride of calcium began to absorb moisture from the beginning of storage under all the above conditions relative humidity. A residual moisture content of 1 g of each sample reached 0,364 g at a relative humidity of 33.0% and 0,430 g at a relative humidity of 52.8% after 7-day storage. On the other hand, two kinds of associates of trehalose and calcium chloride almost showed no moisture absorption after 7 days of storage and there was virtually no increase in moisture content. The degree of absorption of moisture of the samples was obviously low compared with the case of calcium chloride. Next, two kinds of associates of trehalose and calcium chloride almost did not show hygroscopicity at macroscopic observation, while the calcium chloride showed her. The above results indicate that the inherent calcium chloride property hygroscopicity decreases associates of trehalose and calcium chloride.

Driedpowder uterine saline began to absorb moisture from the beginning of storage under all the above conditions relative humidity. A residual moisture content of 1 g of each sample has reached a high level 0,398 g at a relative humidity of 33.0% and 0,523 g at a relative humidity of 52.8% after 7-day storage. Another from the pile, a residual moisture content of the powder, including associate trehalose and components uterine saline, reached 0,175 g at a relative humidity of 33.0% and 0,356 g at a relative humidity of 52.8% after 7-day storage. The magnitude of the moisture content of the samples were significantly low compared with the case of the dried powder uterine saline. Next, the powder including associate trehalose and components uterine saline, showed no hygroscopicity at macroscopic observation, while the dried powder uterine saline showed her. The above results indicate that the inherent dried powder uterine saline property hygroscopicity decreases in the powder, including associates of trehalose and components uterine saline. Property listed above associates of trehalose and components uterine salt solution can preferably be used for the production of dried sea food, i.e. dried seaweed, such as dried kelp, dried brown seaweed, dried Ulva, red algae, and the like, dried whole fish or dried gutted fish, such as flying fish, Barracuda, sillogue fish, horse mackerel, mackerel, "hockey", sardine, macreedy, plural, comb the La, octopus, squid and the like, hygroscopicity which decreases with steps ensure contact of the marine food products with trehalose in aqueous solution, preferably with a solution of trehalose with a concentration of trehalose 2% (wt./wt.) or higher, forming associat trehalose and components uterine saline solution contained in the marine food products, and drying the mixture to obtain a dried sea foods, such as seaweed and fish.

Experiment 5

The inhibitory effect of trehalose and maltitol to precipitate calcium phosphate

When ion phosphate is added to aqueous solution of calcium chloride from calcium ion and ion phosphate is formed and precipitates insoluble salt of calcium phosphate. Effects of trehalose, maltitol and other saccharides on the phenomenon investigated in the following way. An aqueous solution of calcium chloride was prepared by stepwise addition of 3,68 g of the dihydrochloride of calcium to deionized water to dissolve it and add deionized water to the solution to a total volume of 200 ml. of Water crystalline trehalose, anhydrous crystalline maltitol, water crystalline maltose and anhydrous crystalline sucrose was used as saccharides for testing. Solution phosphate (pH 6.8) was prepared by sequential smeshivaniem ml of 0.2 M solution of potassium dihydrophosphate and 118 ml of 0.2 M solution of sodium hydroxide to bring the resulting solution to 1 l with deionized water.

26 g of any of the sugars (STA) test was added to 5 ml of the above solution of calcium chloride and dissolved by adding an additional amount of deionized water. The resulting aqueous solutions were added to obtain a total volume of 50 ml of the Control solution was prepared by stepwise addition of deionized water to 5 ml of the above solution of calcium chloride and addition of the resulting solution with deionized water to a total volume of 50 ml. and Then 40 ml of the above phosphate solution was mixed with 10 ml of a solution of calcium chloride. After stirring at 37°C for 3 h in each solution was centrifuged at 10,000 rpm for 10 min and collected the obtained supernatant. The calcium concentration (concentration solubilizing calcium) in each supernatant was measured with the use of device "ZEEMAN 5100 atomic adsorption spectrophotometer sold by Perkin-Elmer Japan Co. Ltd., Kanagawa, Japan. Samples for measurements were prepared by stepwise addition of 2 ml of 10% (wt./about.) solution of lanthanum chloride to 5 ml of each of the above supernatant fluid after centrifugation and addition of deionized water to the resulting solution to 25 ml

The above procedure was performed for each individual sample (4 types of saccharides and control) 3 times and calculated the average led the rite concentration solubilizing calcium. The results are summarized in table 9.

Table 9
SaccharideConcentration solubilizing calcium (mg/l, average ± standard deviation)
Without saccharides (control)6,86 ± 0,49
Trehalose23,90 ± 2,54
Maltitol20,13 ± 1,17
Maltose6,79 ± 0,52
Sucrose6,54 ± 0,31

As shown in table 9, it was found that trehalose and maltitol possessed the ability to inhibit the precipitation of calcium phosphate, which is formed in the joint presence of calcium ion and ion phosphate. Based on the results obtained in experiments 1-3, which showed the formation of associates of trehalose and maltitol with compounds containing metal ions, believed that trehalose and maltitol inhibited the formation of insoluble salts (calcium phosphate), formed by ionic bond between the calcium ion and ion phosphate Association with solubilizing salt of calcium (in this experiment, calcium chloride).

Experiment 6

The effect of trehalose and maltitol to improve the solubility of the calcium salt of the organic acid

Dei is the influence of trehalose, maltitol and other saccharides on the solubility of calcium salts of organic acids was investigated as follows. Three types of solutions of sugars, including various sugars was obtained by dissolving 5 g (STO) trehalose, maltitol and maltose in 35 ml of deionized water. Then 5 g of a commercially available, pentahedra-DL-calcium lactate or monohydrochloride calcium, used as the calcium salts of organic acids, was added to each solution of the saccharide with the subsequent suspendirovanie. Then the pH of each solution was brought to 3.5, and the solution was added with deionized water to a volume of 50 ml After stirring at 25°C for 16 h insoluble calcium salts of organic acids were removed by centrifugation (15,000 rpm for 30 min). The measured pH of the obtained supernatant liquids. The calcium concentration in the supernatant was measured using atomic adsorption photometer described in experiment 5. In the solution prepared without sugar, the concentration of calcium was also measured in the same manner as in the control. The results are presented in table 10.

Table 10
Calcium lactateCalcium gluconate
pHThe concentration of CA (mg/ml)Relative concentration (%)pHThe concentration of CA (mg/ml)Relative concentration (%)
Without sugars3,710,441003,74,54100
Trehalose3,713,461293,75,07112
Maltitol3,712,821233,7of 5.83128
Maltose3,710,19983,74,73104

As shown in table 10, the pH of all the supernatant fluid was 3.7. The concentration of calcium in the supernatant fluids were increased with the addition of trehalose or maltitol. In the case of calcium lactate calcium concentration in the supernatant increased by 29% in the case of trehalose and 23% in the case of maltitol compared with the concentration of calcium in the control. In the case of calcium gluconate calcium concentration in the supernatant increased by 12% in the case of trehalose and 28% in the case of maltitol compared with the concentration of calcium in the control. It was found that trehalose and maltitol by abudalipovna improve the solubility of the calcium salts of organic acids, such as calcium lactate and calcium gluconate. The function of trehalose and maltitol can be used to prevent clouding of soft drinks, including calcium salts of organic acids, sports drinks and drinks with mineral additives, which occurs during storage for a long period of time.

Experiment 7

The effect of suppression of trehalose and maltitol oxidation of ferrous iron ion

In General, ion of divalent iron (ferrous, Fe2+) and ferric ion (ferric, Fe3+) is known as the ions of the element iron. Ion ferrous iron is easily oxidized by light or heat and is converted into ferric ion. Effects of trehalose and maltitol on the phenomenon investigated in the following way. An aqueous solution comprising tetrahydrochloride ferrous iron (FeCl2)corresponding to the amount of 1% (wt./about.) in the form of ferrous iron ion, and including trehalose or maltitol, corresponding to the amount of 5% (wt./vol.), (STO), was prepared as the test solution. In addition, as a control solution was prepared a solution comprising chloride ferrous iron with the same concentration as the test solution. After preparation of the test and control solutions, small particolored respectively were taken as samples the number of bivalent iron ions was measured as described below Nitroso-DMAP. Then 10 ml each of the test and control solution were placed separately in 20 ml vial and sealed. After storing these bottles at 37°C for 4 h with irradiation of light of approximately 9000 Lux amount of ferrous iron ion in each solution was measured by the method Nitroso-DMAP. Measurement method Nitroso-DMAP was carried out as follows. After the exact 100-fold dilution of the test or control solution 0.5 ml of the diluted solution was placed in a 50 ml volumetric flask. Then 5 ml of 0.2% (wt./about.) nitroso-dimethylaminophenol in 0.1 n solution of hydrochloric acid and 4 ml of 3 n ammonium buffer (pH 8.5) was quickly added to the diluted solution and gently complemented with deionized water to a volume of 50 ml After the above procedure was measured spectral absorption capacity of the solution at 750 nm (within the visible light range). Standard solutions obtained stepwise dilution of a solution of chloride ferrous iron of known concentration used to measure the same way with the generation of a standard curve for quantitative analysis. The number of ions of bivalent iron in the test or control solution was determined from the standard curve. The results are presented in Alice 11.

Table 11
SaccharideThe number of ion Fe2+(mg/ml)
Before light irradiationAfter light irradiation
Trehalosethe 10.14,4
Maltitol10,24,3
Without saccharides (control)10,2the 3.8

As shown in table, in a test solution comprising trehalose or maltitol, ion ferrous iron remained in apparently large numbers after light irradiation compared with its number in the control solution. Taking into account these results and the results of the experiment 2-2, which showed the formation of associate of trehalose and iron salts, believed that the above inhibitory effect of trehalose and maltitol is the result of the formation of associates of these sugars and salts of iron.

Experiment 8

The overwhelming influence of trehalose and maltitol on the destruction of ascorbic acid in the presence of metal ion

L-ascorbic acid is rapidly destroyed due to oxidative decomposition in the presence of iron ions and copper and cause darkening. Effects of trehalose and small is titoli on the phenomenon studied as follows. Prepared 10 kinds of aqueous solutions having the respective compositions described in table 12. Aqueous solutions of one L-ascorbic acid or L-ascorbic acid and compounds containing metal ions, were used as controls. The tested solutions were prepared by adding trehalose or maltitol to the test solutions. 10 ml each of these subjects and control solutions were placed in different 20-ml vials and sealed. The bottles were kept at 50°C. Control and tested solutions containing chloride ferrous iron, and solutions containing chloride ferrous iron or copper sulfate, kept respectively within 96 h and 40 h After storage was measured by the degree of staining of each solution. In the case of the control solution containing one L-ascorbic acid, the degree of staining was measured after storage periods of 40 h and 96 h of the Spectral absorption capacity at 420 nm (within the visible light range) of each sample was measured as an estimate of the degree of staining. The results are presented in table 12.

Tablica
The composition of the solution*NoteThe degree of coloring (Will.** at 420 nm)
Storage is s for 40 h Storage for 96 h
14,8 mm AsAControl0,0210,166
14,8 mm AsA + 10 mm CuSO4< / br>
14,8 mm AsA + 10 mm CuSO4+ 100 mm Tre

14,8 mm AsA + 10 mm CuSO4+ 100 mm Mal
Control

Test

Test
0.800 to

0,311

0,258
-

-

-
14,8 mm AsA + 10 mm FeCl3< / br>
14,8 mm AsA + 10 mm FeCl3+ 100 mm Tre

14,8 mm AsA + 10 mm FeCl3+ 100 mm Mal
Control

Test

Test
0,529

0,226

0,198
-

-

-
14,8 mm AsA + 10 mm FeCl2< / br>
14,8 mm AsA + 10 mm FeCl2+ 100 mm Tre

14,8 mm AsA + 10 mm FeCl2+ 100 mm Mal
Control

Test

Test
-

-

-
0,588

0,380

0,291
* Designate "AsA", "Tre" and "Mal" respectively mean L-ascorbic acid, trehalose and maltitol.

** Spectral absorption capacity

As shown in table 12, the degree of staining of test solutions containing trehalose and maltitol, were significantly lower compared with staining control. Given these results and the results of the experiment 2-2, which showed the formation of associate of trehalose and iron salts or salts of copper, believe that the above hearth is allowing the effects of trehalose and maltitol are the result of the formation of associates of these sugars and salts of iron and copper salt.

Experiment 9

The inhibitory effect of trehalose on the formation of scale when boiling food

The scum formed by boiling one meat or meat with vegetables. When the formation of scale in excess, it should be removed. The effect of trehalose on the above phenomenon was studied as follows. 30 g of pork and 20 g of spinach was added to 400 ml of water, and then thereto was mixed into 2% (wt./wt.) or 10% (wt./wt.) sucrose. Then the mixture was heated, boiled for 1 min, stop heating, and then cooled to room temperature. After cooling, the scale was allocated a sequential collection on the filter KIRIYAMA" by washing with 500 ml deionized water and drying at 40°C for 18 h Scale, obtained in a similar manner without the use of a saccharide, was used as control. Measured the mass scale and the number of components contained in the scale. The results are presented in table 13.

Table 13
SaccharideNumber % (wt./wt.)Weight scaleFat (g)Protein (g)Ash (g)CA (mg)Mg (mg)
(g)Rel. wt.* %)
Trehalose20,506460,4060,0760,009only 1,613 Torino0,810
100,315290,2440,0480,0051,5930,156
Sucrose21,060970,8710,1700,0131,6722,475
Without sugars01,0911000,8990,1660,0121,3262,550
* The relative mass (%)

As shown in table 13, weight scale decreased with increasing amounts of trehalose. At the same time, the sucrose had no effect reduce the number of scale. The amount of scale formed by the use of sucrose, was almost the same as in the control. From the obtained results it was found that trehalose have the property of suppressing the formation of scale. From the analysis of the components of scale suggest that the calcium ion and magnesium ion deeply involved in the inhibition of scale formation trehalose. It is also suggested that trehalose inhibits the formation of insoluble compounds containing ions of m is fester, in particular magnesium salts of fatty acids.

Experiment 10

The inhibitory effect of trehalose and maltitol on the formation of scale during cooking boiled food

Steps trehalose and maltitol on the formation of scale compared with the effects of other saccharides as follows. After adding 30 g of pork to 400 ml of each solution consisted of 10% (wt./wt.) one of trehalose, maltitol, NetRegulus, sucrose, maltose or glucose, and soaking for 10 min the solution was heated and boiled for 5 minutes After stopping the heating and cooling to room temperature the resulting scale was allocated Paladino collecting on the filter KIRIYAMA", by washing with 500 ml deionized water and drying at 40°C for 18 h Scale, obtained in a similar manner without the use of a saccharide, was used as control. Measured the mass scale and the number of minerals (calcium, magnesium, sodium and potassium)contained in the scale. The results are presented in table 14.

(%)
Table 14
Weight scaleCalciumMagnesiumSodiumPotassium
(g)(mg)(%)(mg)(%)(mg)(%)(mg)(%)
Without sugars2,2521000,1771000,0561000,2471000,023100
Trehalose0,579260,048410,01120of 0.182740,01774
Maltitol0,802360,030260,029520,183740,01565
NetRegulus1,942860,090770,042750,237960,024104
Sucrose1,718760,113970,052930,2991210,025109
Maltose2,126940,092790,050890,2831150,025109
2,17196amount of 0.1181010,04886to 0.2631060,01983

As shown in table 14, in the case of trehalose or maltitol mass of educated scale was reduced to less than half in the case of the control (without sugar). At the same time NetRegulus, sucrose, maltose and glucose had no effect on reducing the number of scale. The number formed by scaling by the use of these sugars was almost on the same level as in the control (without sugar). The analysis of minerals contained in the scale, it was found that the content of calcium and magnesium was low. Suggest that trehalose and maltitol inhibit the formation of scale suppression of the formation of insoluble compounds containing calcium ions and magnesium.

Experiment 11

The inhibitory effect of trehalose on the elution of magnesium from food, subjected to boiling during cooking

The corresponding filtrates obtained by removing scale in experiment 10 was added water to obtain a total volume of 500 ml and measured the amount of minerals (calcium, magnesium, sodium and potassium)contained in the filtrate. The results are presented in table 15. Consider that the total number mi is the Eraly, buervenich of 30 g of pork, can be calculated by summing the values in table 14 (the number in the scale) and the values in table 15 (the number in the filtrate). Therefore, the total number and relative values calculated using the control values (without sugar) as having 100 parts presented in table 16.

Table 15
CaMgNaK
(mg)%(mg)%(mg)%(mg)%
Without saccharide0,9641001,1581003,4421006,325100
Trehalose0,788820,485423,4901016,03595
Maltitol1,4251481,5231323,412996,15097
NetRegulus0,957991,148993,8801136,060 96
Sucrose0,9771011,150993,420996,16597
Maltose0,942981,152993,4371006,16097
Glucose1,0511091,149993,4261006,28599
Table 16
CaMgNaK
(mg)%(mg)%(mg)%(mg)%
Without saccharide1,0811001,2141003,6891006,348100
Trehalose0,836770,496413,6721006,05295
Maltitol1,4551351,5521283,595976,16597
NetRegulusat 1,047971,190984,1171126,08496
Sucrosekgs 1,0901011,202993,7191016,19098
Maltoseto 1.034961,202993,7201016,18597
Glucose1,1691081,197993,6891006,30499

As shown in table 16, in the case of sugars, except for trehalose and maltitol, the total number of relevant minerals, buervenich pork, were almost the same as in the case of the control (without sugar). At the same time, especially in the case of trehalose, magnesium was less than half of control, and trehalose inhibited the elution of compounds containing magnesium ions. On the other hand, in the case of maltitol quantities of calcium and magnesium, erwerbende pork, were greater than in control, and maltitol contributed to the elution of pork compounds containing calcium ions and magnesium. According to the obtained results suggested that multi the ol inhibits the formation of scale due to the suppression of the insolubility of the compounds, containing calcium ions and magnesium, even though maltitol promotes their elution from pork. It is also suggested that trehalose inhibits the formation of scale suppression of elution of pork compounds containing ions of calcium and magnesium, in particular compounds containing magnesium ions.

Experiment 12

The inhibitory effect of trehalose on the elution of magnesium from vegetables subjected to boiling during cooking

The inhibitory effect of trehalose on the elution of magnesium was studied on the vegetables. After adding 20 g shungiku" (chrysanthemum Daisy chained) or spinach to 400 ml of an aqueous solution comprising 10% (wt./wt.) trehalose and soaking for 10 min, the solution was heated and boiled for 5 minutes After stopping the heating and cooling to room temperature the resulting scale was allocated Paladino collecting on the filter KIRIYAMA, washing the collected scum 500 ml deionized water and drying at 40°C for 18 hours Mass scale and the amount of minerals contained in the scale, measured in the same manner as in example 9. The filtrate obtained by removing scale, supplemented with water to a total volume of 500 ml, and the amount of minerals (calcium, magnesium, sodium and potassium) were measured in the same manner as in experiment 10. Samples prepared without sugar, and the samples prepared using the-W sucrose instead of trehalose, used respectively in quality control 1 and 2. The total quantity of minerals contained in the scale and the filtrate was determined as the number of minerals, buervenich of vegetables during cooking. The amount of minerals contained in the scale and the filtrate is presented respectively in tables 17 and 18. The sum of these values (the total number buervenich minerals) are presented in table 19.

Table 17
VegetableSaccharideWeight scaleCalciumMagnesiumSodiumPotassium
(g)(%)(g)(%)(g)(%)(g)(%)(g)(%)
Chain chrysanthemumWithout saccharide0,0141000,4101000,0921000,1971000,380100
Trehalose0,008570,156380,034370,074370,30 79
Sucrose0,010710,299730,059630,05829in 0.28876
SpinachWithout saccharide0,0341001,1401000,8231000,8301001,204100
Trehalose0,022650,553480,501610,727880,80467
Sucrose0,022650,886780,542640,461560,72660

Table 18
VegetableSaccharideCalciumMagnesiumSodiumPotassium
(mg)(%)(mg)(%)(mg)(%)(mg)(%)
Chain chrysanthemumBe the saccharide 13,1091001,2991003,2311000,707100
Trehalose11,928910,628483,314103of 0.61587
Sucrose12,683971,4401113,213990,65593
SpinachWithout saccharide4,4261006,9861001,9541000,778100
Trehalose3,850873,264471,879960,75297
Sucrose4,6041046,9541001,918980,825106

Table 19
VegetableSaccharideCalciumMagnesiumSodiumPotassium
(mg)(%)(m is) (%)(mg)(%)(mg)(%)
Chain chrysanthemumWithout saccharide13,1091001,2991003,2311000,707100
Trehalose11,928910,628483,314103of 0.61587
Sucrose12,683971,4401113,213990,65693
SpinachWithout saccharide4,4271006,9871001,9551000,779100
Trehalose3,851873,265471,879960,75397
Sucrose4,6051046,9551001,919980,825106

As shown in tables 17, 18 and 19, it was revealed that trehalose inhibited the formation of scale from "shungiku" (chrysanthemum Daisy chained) and spinach and, in particular, the elution of the compounds, aderrasi magnesium ions. Trehalose showed the ability to suppress the elution of compounds containing magnesium ions, from vegetables during cooking, as in the case of meat, described in experiment 11. Turning to the color of chrysanthemum Daisy chained and spinach after boiling, boiled vegetables, prepared with trehalose, well retained its green color compared to vegetables cooked without the use of a saccharide and using sucrose.

Experiment 13

The inhibitory effect of trehalose on the elution of magnesium from Japanese wheat noodles in boiling

The inhibitory effect of trehalose on the elution of magnesium was investigated in a boiling Japanese wheat noodles. 5 g fresh Japanese wheat noodles) was added to 40 ml of an aqueous solution comprising 10% (wt./wt.) trehalose, heated and cobatillas within 2 minutes After cessation of heating and cooling to room temperature, the noodles were removed and filtered through glass fiber filter. The obtained filtrate was added water to a total volume of 50 ml. Among the minerals contained in the filtrate was measured only the amount of magnesium in the same manner as in experiment 10. The filtrate is made without sugar, used as a control. The results of measuring the amount of magnesium contained in the leachate are presented in table 20.

Table 20
SaccharideMagnesium
(g)(%)
Without saccharide162,6100
Trehalose51,948

As shown in table 20, it was revealed that trehalose inhibited the elution of compounds containing magnesium ions, from Japanese wheat noodles during boiling. Trehalose showed the ability to suppress the elution of compounds containing magnesium ions, from Japanese wheat noodles during boiling, as in the case of meat and vegetables, described respectively in experiments 11 and 12.

The following examples explain in detail associates of the present invention and their use.

Example 1

Associat trehalose and calcium chloride

In accordance with the method described in experiment 1-1 was obtained crystals of two types of associates of trehalose and calcium chloride in a molar ratio of 1:1 and 1:2. The tablet received in the usual way by mixing 2.5 mg of each of the drugs with 200 mg of potassium chloride as a filler and molding the mixture into a tablet. Absorption spectra of infrared light drugs was measured using an infrared spectrophotometer Fourier transform FT-IR 8200". The results present the Lena, accordingly, figure 5 and 6.

Because of the above products have a lower hygroscopicity, they are characterized by a satisfactory ability to manipulate when storing or mixing with different compositions. Further, as the above products are unlikely to form insoluble salt, such as calcium phosphate, mixed with a composition comprising phosphoric acid, a phosphate salt or ion phosphorus, final products, turbidity or sedimentation which was prevented, can be obtained from the use of products containing aqueous solutions with calcium, such as isotonic drinks, nutritional supplements and drugs for external application with application to the skin. So the above crystals of two types of associates of trehalose and calcium chloride is very useful as components of foods with calcium in various areas of food production, cosmetics, pharmaceuticals, etc.

Example 2

The associates of trehalose and various compounds containing metal ions

One part of the masses. (part of mass.) aqueous crystalline trehalose was mixed with equimolar with trehalose quantities of each packagedownload magnesium, hexahydroborite strontium, tetrahydrochloride ferrous iron, tetrahydrochloride copper, hexahedronal the Nickel or hexahydroborite manganese, mixed with 0,53 h masses. deionized water and completely dissolved by heating. After cooling, each resulting solution was dried in vacuum at 80°C for 15 hours 7 species of powdery composites were obtained by grinding into a powder obtained from the dried materials. Take respectively of the samples and used for the analysis of the absorption spectrum of infrared light in accordance with the method described in example 1. The results are presented respectively in Fig.7-12.

Since the solubility of these complexes in water is improved compared with the same compounds containing metal ions, final products, turbidity and sedimentation are prevented, can be obtained by using associates as components of aqueous solutions with compounds containing metal ions, such as isotonic drinks, nutritional supplements and drugs for external application with application to the skin. Therefore, the associates are very useful as components for products with compounds containing metal ions, in different areas of food production, cosmetics, pharmaceuticals, etc.

Example 3

Associates of maltitol and various compounds containing metal ions

One part of the masses. anhydrous crystalline maltitol mixed takvimleriydi with multicolor quantities of each of the dihydrochloride of calcium or tetrahydrochloride ferrous iron, mixed with 0,53 part of the mass. deionized water and completely dissolved by heating. After cooling, each resulting solution was dried in vacuum at 80°C for 15 h 2 types of powder composites were obtained by grinding into a powder obtained from the dried materials. Take respectively of the samples and used for the analysis of the absorption spectrum of infrared light in accordance with the method described in example 1. The results are presented respectively on Fig and 14.

Since the solubility of these complexes in water is improved compared with the same compounds containing metal ions, final products, turbidity and sedimentation are prevented, can be obtained by using associates as components of aqueous solutions with compounds containing metal ions, such as isotonic drinks, nutritional supplements and drugs for external application with application to the skin. Therefore, the associates are very useful as components for products with compounds containing metal ions, in different areas of food production, cosmetics, pharmaceuticals, etc.

Example 4

Powdered isotonic drink

In accordance with the following composition is a powder composition was prepared mix is m sufficient amounts of each component.

Aqueous crystalline trehalose6000 h wt.
Sucrose5000 h wt.
Vitamin B10,1 h wt.
Vitamin B20,3 h wt.
Vitamin B60,4 h wt.
Vitamin C200 h wt.
Niacin4 h wt.
Centripetal (anhydrate)93 h wt.
Potassium phosphate (anhydrate)62 o'clock mass.
Associat trehalose and magnesium chloride obtained by the method of example 290 h wt.
Associat trehalose and calcium chloride (molar ratio 1:2)obtained by the method of example 155 h wt.

The above powder composition was distributed in 200 ml plastic bottles with screw-down lids, for the manufacture of powdered isotonic drinks. The product can be used after adding approximately 100 ml of water to 10 g of the product and dissolution. Since the product contains the associates of trehalose and compounds containing metal ions, have properties of low hygroscopicity, the product can be stored for a long period of time. Also associates contained in the product, convenient is, because they dissolve quickly in water. Further, since associat trehalose and calcium chloride contained in the product, hardly forms an insoluble salt of the phosphate ions and precipitates when dissolved in water, the product is characterized by a low probability of deterioration of the absorptivity of each component, when a solution of the product drink after water and storing it for a relatively long time.

Example 5

Skin lotion (Outer skin lotion)

In accordance with the following composition to obtain a liquid composition by mixing and dissolving.

Citric acid0,02 h wt.
Sodium citrate0,08 h wt.
1,3-butyleneglycol2 h wt.
Ethanol2 h wt.
Anhydrous crystalline maltitol1 h wt.
Aqueous crystalline trehalose0.2 h wt.
2-glucoside L-ascorbic acid0.5 h wt.
Associate of maltitol and chloride ferrous iron, obtained by the method of example 30,0035 h wt.
Purified waterRest
Only100 h wt.

At azanuy above liquid composition was poured into 100 ml glass bottles, fitted with screw-caps, to obtain skin lotions. Because the product provides adequate refreshing feeling and has the ability to retain moisture, it can be used as the primary means for skin care to maintain healthy skin. Because associate of maltitol and chloride ferrous iron contained in the product is unlikely to cause damage to other components described effects can be obtained after storage of the product for a relatively long period of time.

Example 6

Vitamin Supplement

In accordance with the following composition is a powder composition was obtained by mixing sufficient amounts of each component.

Folic acid0,0004 h wt.
L-ascorbic acid0.2 h wt.
Associat trehalose and magnesium chloride obtained by the method of example 25 h wt.
Associat trehalose and manganese chloride, obtained by the method of example 10,008 h wt.
Aqueous crystalline trehalose5 h wt.

The above powder composition distributed in 80 ml glass bottle, supplied davinciaudio the I-lids, to obtain vitamin supplements. The product is taken orally with an approximate rate of about 10 g per day, and it can be drunk after adding approximately 100 ml of water or hot water to 10 g of the product and its dissolution. Because the associates of trehalose and compounds containing metal ions included in the product, can be quickly dissolved in water, the use of the product is very easy.

Example 7

Powder, including associate trehalose and components uterine saline

In accordance with the method described in experiment 2-1, 4 h masses. aqueous crystalline trehalose and 25 h of the masses. commercially available uterine saline sold by Sanuki Engyou Co., Ltd., Kagawa, Japan, were mixed and completely dissolved by heating. The resulting solution was dried in vacuum at 60°C for 15 h, and then the obtained dried substance was crushed into powder to produce a powder that includes associat. In accordance with the method described in example 1 was performed the analysis of the absorption spectrum of infrared light product. The result is shown in Fig.

Because the product has a lower water absorption compared to the control, the dried powder uterine salt solution obtained by the method of experiment 4, it can satisfactorily be manipulated when storing and mixing with the hypoxia compositions. Also, because of the inherent uterine saline solution unpleasant taste, such as caustic taste and bitter taste, was suppressed and improved education of associate with trehalose, the product can be used as mineral fortifier that includes relatively large amounts of magnesium and calcium; material seasonings, isotonic drinks, nutritional supplements, food, pet food; and improves the taste of funds for the production of "Anne" (sweetened bean paste), natto (fermented soybeans), soy milk and tofu (cheese from soy beans); in particular as coagulant for tofu (cheese from soy beans). In addition, the product is very useful as a component in various products in various areas of food production, agricultural and marine products, cosmetic products, pharmaceutical products, etc. as mineral supplementation of minerals such as magnesium and calcium, nutritional supplements for plants, activating agents for plants, agents, moisture retaining agent, suppressing an allergic reaction such as hay fever (pollen disease), etc.

Example 8

Powder, including associate trehalose and components uterine saline

1 h masses. water crystal trega the eskers and 1 h of the masses. commercially available uterine saline sold by Sanuki Engyou Co., Ltd., Kagawa, Japan, were mixed and completely dissolved by heating. The resulting solution was sprayed onto 300 h masses. anhydrous crystalline trehalose and mixed to obtain a dried powder comprising associat.

Because the product has a lower water absorption compared to the control, the dried powder uterine salt solution obtained by the method of experiment 4, it can satisfactorily be manipulated when storing and mixing with different compositions. Also, because of the inherent uterine saline solution unpleasant taste, such as caustic taste and bitter taste, was suppressed and improved education of associate with trehalose, the product can be used as mineral supplementation, including large quantities of magnesium and calcium; material seasonings, isotonic drinks, nutritional supplements, food, pet food; and improve the taste of the agent for the production of "Anne" (sweetened bean paste), natto (fermented soybeans), soy milk and tofu (cheese from soy beans); in particular as a coagulant for "tofu" cheese from soy beans). In addition, the product is very useful as a component in various products in various fields of production is Isaevich products agricultural and marine products, cosmetic products, pharmaceutical products, etc. as mineral supplementation of minerals such as magnesium and calcium, nutritional supplements for plants, activating agents for plants, agents, moisture retaining agent, suppressing an allergic reaction such as hay fever (pollen disease), etc.

Example 9

The solution includes associat trehalose and components uterine saline

In accordance with the method described in experiment 2-1, 144 h masses. aqueous crystalline trehalose and 202 h masses. commercially available uterine saline sold by Sanuki Engyou Co., Ltd., Kagawa, Japan, were mixed and completely dissolved by heating. The resulting solution was concentrated under reduced pressure, and then at 60°C for 15 hours, and then received a concentrated solution containing 63% (wt./wt.) dry solids.

Because of the inherent uterine saline solution unpleasant taste, such as caustic taste and bitter taste, was suppressed and improved education of associate with trehalose, the product can be used as mineral supplementation, including large quantities of magnesium and calcium; material seasonings, isotonic drinks, nutritional supplements, the product is in the food and pet food; and improve the taste of the agent for the production of "Anne" (sweetened bean paste), natto (fermented soybeans), soy milk and tofu (cheese from soy beans); in particular as a coagulant for tofu (cheese from soy beans). In addition, the product is very useful as a material of different products in different areas of food production, agricultural and marine products, cosmetic products, pharmaceutical products, etc. as mineral supplementation of minerals such as magnesium and calcium, nutritional supplements for plants, activating agents for plants, agents, moisture retaining agent, suppressing an allergic reaction such as hay fever (pollen disease), etc.

Example 10

Powder, including associate of maltitol and components uterine saline

2 h masses. anhydrous crystalline maltitol and 1 h of the masses. commercially available uterine saline were mixed and completely dissolved by heating. The resulting solution was dried in vacuum at 80°C for 15 h, and then the obtained dried substance was crushed into powder to produce a powder that includes associate.

Because the product has a lower water absorption compared to the control, the dried powder uterine saline, polucen the m by way of experiment 4, they can satisfactorily be manipulated when storing and mixing with different compositions. Also, because of the inherent uterine saline solution unpleasant taste, such as caustic taste and bitter taste, was suppressed and improved education of associate with multicolor, the product can be used as mineral supplementation, including a relatively large amount of natural magnesium and calcium; material seasonings, isotonic drinks, nutritional supplements, food, pet food; and improve the taste of the agent for the production of "Anne" (sweetened bean paste), natto (fermented soybeans), soy milk and tofu (cheese from soy beans); in particular, as a coagulating agent for "tofu" cheese from soy beans). In addition, the product is very useful as a component in various products in various areas of food production, agricultural and marine products, cosmetic products, pharmaceuticals, etc.

Experiment 11

Table salt

In accordance with the following composition solids was obtained by mixing sufficient amounts of each component and drying under reduced pressure. The obtained solids were crushed to obtain powder table salt.

Sodium chloride90 h wt.The solution containing associat trehalose and components uterine salt solution obtained by the method of example 912 h wt.

Table salt has a low hygroscopicity and a satisfactory flowability. Because of the unpleasant taste of the fallopian salt solution, such as caustic taste and bitter taste associated with sodium chloride and components uterine saline, was suppressed, and the product has a good taste due to adequate harmonization of sodium chloride, trehalose and components uterine salt solution, the product can be used for cooking and as a condiment for food products (including food, cooked on the grill and drinks, and he gets their taste. Also because the product has a composition similar to the components of sea water, it is a mild salt for living organisms. For example, an aqueous solution comprising the product at a concentration of about 3%can mainly be used to remove sand from a sea animal with a shell.

Example 12

Salt for seasoning with low content of sodium chloride

In accordance with the following composition salt for seasoning with low content of sodium chloride were prepared by mixing rises the amount of each component.

Sodium chloride60 h wt.
Potassium chloride9 h wt.
Monosodium L-glutamate1 h wt.
Powder, including associate trehalose and components uterine salt solution obtained by the method of example 85 h wt.

Salt for seasoning with low content of sodium chloride has a low hygroscopicity and a satisfactory flowability. Suppressed the bitter taste of the product originating from the components of the uterine saline. Because the product includes sodium chloride, trehalose, potassium chloride and monosodium L-glutamate, the overall taste of the product is improved in addition to salt to taste. The product can be used to improve the palatability of foods and beverages, as well as conventional products, although the product has a low content of sodium chloride, and it gets their taste. Also the product can preferably be used to facilitate the recovery of patients with diseases of the circulatory system, preventing disease in adults and, in addition, to maintain and promote health and beauty.

Example 13

"Ann" (sweetened bean paste)

In accordance with the following song "Anne" (sweetened bean is a new paste) prepared by mixing each component and processing.

Commercially available white raw pasta "Ann"1000 h wt.
Sucrose700 h wt.
"SUNMALT-S (water crystalline maltose)100 h wt.
Starch syrup (75% (wt./wt.) dry solids)100 h wt.
Powder containing associat trehalose and components uterine salt solution obtained by the method of example 8100 h wt.
Agar2.5 h wt.

Because "Anne" includes trehalose and components uterine salt solution, in particular magnesium, it has a satisfactory taste, color, storage, and low sweetness. "Ann" is preferred for Japanese confectionery such as "Monica" (Japanese cake).

Example 14

Processed soy milk

Processed soy milk received in accordance with the following procedure. Remove the peel with 10 h of the masses. substances soybeans. After autoclaving at 130°C for 10 min to the beans during grinding was added 90 h of the masses. the hot water. About 60 h of the masses. soy milk was obtained by removal of the residue (pomace cheese from soy beans) from a solution mixture by centrifugation. 10 h masses. maltodextrin (DE (dextrose equivalent is alent) 20), 5 h masses. "SUNMALT®"powdery crystalline maltose sold by Hayashibara Shoji Inc., Okayama, Japan, 0,05 h masses. table salt obtained by the method of example 11, 0,02 h masses. the soybean oil and a suitable amount of lecithin was added to the soy milk and dissolved. Processed soy milk received stages sterilization of the above mixture by heating, deodorizing vacuum, mixing with the appropriate amount of flavoring, homogenization, cooling, pouring into a vessel and wraps.

Unlike conventional similar soy milk, processed soy milk includes trehalose and a small amount of magnesium. Therefore, it is a drink that provides a good taste without the bitter, sharp taste and scratchy feeling and that you can drink in one gulp.

Example 15

"Tofu" cheese from soy beans)

"Tofu" cheese from soy beans) received in accordance with the following procedure. 1 h masses. soybeans were washed with water, soaked in water for 12 hours, and then crushed. After adding 5 h masses. water to the crushed material and boiling for 5 min the mixture was filtered through cloth to obtain soybean milk. "Tofu" received sequential addition of 1 h masses. each pullulan and powder, which includes associat was trihalo the s and components uterine saline, obtained by the method of example 7 to 100 h of the masses. soy milk, as a coagulating agent in the 70°calling him coagulation.

Processing efficiency in the production of this "tofu" is increased, because the time required for coagulation of soy milk, extends to approximately 7 min, compared with the case of using uterine saline. As "tofu" includes pullulan and trehalose, he manifests low syneresis and high output, and has a satisfactory texture, gloss and fragrance. The product has a satisfactory stability during storage, and it can be used for the preparation of chilled tofu, cut into cubes, boiled tofu, soup miso".

Example 16

Film pullulan

An aqueous solution of a material for films, including pullulan received in accordance with the following composition and removing bubbles under reduced pressure. Then the film pullulan thickness of 30 μm was obtained stages of continuous pouring the solution on a synthetic plastic plate and drying by hot air having a temperature of 60°C.

"PULLULAN PI-20", product of pullulan sold by Hayashibara Shoji Inc., Okayama, Japan1000 h wt.
Ester of sugar (mon who laurate sucrose) 1 h wt.
The solution containing associat trehalose and components uterine salt solution obtained by the method of example 920 h wt.
Purified water3400 h wt.

Film pullulan has a satisfactory stability to the change of humidity and solubility in water. Further, the film has good taste, because it includes associat trehalose and components uterine saline. The film can be used as a food product and material for recycling. In addition, because the product includes components uterine saline, it can be used as mineral supplements minerals such as magnesium, calcium and potassium, and agent, suppressing allergic reactions such as rhinitis and edema of the mucosa of the nasal cavity caused by hay fever (pollen disease).

Example 17

The feed mixture

In accordance with the following composition of the feed mixture was prepared by mixing each component.

Fish meal
Powdered gluten40 h wt.
Skim milk38 h wt.
Lactosucrose12 h wt.
Vitamin agent10 h wt.
5 h wt.
Diphosphate calcium5 h wt.
Liquid fat3 h wt.
Calcium carbonate3 h wt.
Sodium chloride2 h wt.
Powder containing associat of maltitol and components uterine salt solution obtained by the method of example 102 h masses.

The above feed mixture is difficult denaturised, because the powder that includes associate of maltitol and components uterine saline, which is admixed as minerals of the composition is not very hygroscopic. The product is characterized by a large preference in domestic animals and poultry, in particular in pigs. The product has the function of promoting growth of bifidobacteria, and it can mainly be used to prevent infection and diarrhea in animals, stimulation of appetite, fat storage and they suppress the smell of their excrement. The product can optionally be mixed with other feed materials, such as grain, wheat flour, starch, oil sludge, and molasses for the manufacture of enriched feed. The product can also be used along with the raw feed materials, such as straw, hay, squeezed sugar t is hostnik and the core of the corn cobs, for the manufacture of other feed mixtures.

Example 18

Cosmetic cream

In accordance with the following composition components are mixed and processed to obtain a cosmetic cream. 2 h masses. the monostearate polyoxyethyleneglycol, 5 h masses. self-emulsified monostearate of glycerol, 2 h masses. "αG-HESPERIDINE®", α-glucosylceramide sold by Hayashibara Shoji Inc., Okayama, Japan, 1 h masses. liquid paraffin, 10 h masses. troctanoouli glycerol and the appropriate amount of preservative were mixed and dissolved with heating in the usual way. 2 hours and masses. L-sodium lactate, 5 h masses. 1,3-butyleneglycol, 2 h masses. powder, including associate of maltitol and components uterine salt solution obtained by the method of example 10, and 66 h of the masses. deionized water was mixed into the above mixture and was emulsiable using a homogenizer. To the obtained mixture was further mixed into a suitable number of flavouring substances and stirred for manufacturing cosmetic cream. The product has properties moisture retention, because it includes maltitol and magnesium, and it can be used as a means of protecting against sunburn and skin care and moisturizing agent.

Example 19

Healing ointment (a drug for external use)

LECHEBNO the ointment (a drug for external use) was prepared by mixing the components according to the following composition. Mixed 200 h masses. powder, including associate trehalose and components uterine salt solution obtained by the method of example 8, 300 h masses. maltose and 50 h of the masses. methanol, which includes 3 hours of the masses. of iodine. Next, to 200 h of the masses. an aqueous solution containing pullulan in the amount of 10% (wt./wt.) was mixed into the above mixture for the manufacture of ointments for the treatment of wounds, which has adequate property, ensuring its application to the wound surface, and coupling with it.

Because the product includes associat trehalose and components uterine saline, it includes minerals originating from the uterine saline. In addition to the disinfecting activity of iodine, maltose contained in the product, can be used as adds energy agent for cells. Therefore, the use of the product provides the possibility of shortening the treatment period and satisfactory healing of wounds.

Example 20

A nutritional Supplement for plants

Nutritional Supplement for plants in liquid form were prepared by mixing the components according to the following composition.

td align="left"> The solution containing associat trehalose and components uterine salt solution obtained by the method of example 9
Diammonium phosphate132 h wt.
Ammonium nitrate17,5 h wt.
Potassium chloride71,5 h wt.
360 h wt.
Water1000 h wt.

Product includes nitrogen (N), phosphate (P2O5), potassium (K2O) and magnesium (MgO) in the ratio 10:20:15:3. The product has an activity of promoting plant growth and root germination, when plants take root or change, and improve flowering, flowers and fruit yield. The product can be used with the appropriate dilution as a nutritional Supplement for plants, such as crops, including cereals and potatoes, vegetables, fruit trees, garden plants, garden and roadside trees, the grass on the Golf course.

Example 21

Means for baths

Means for baths were prepared by mixing the components according to the following composition.

Sodium bicarbonate80 h wt.
The dried sodium sulfate12 h wt.
Potassium chloride4 h wt.
Precipitated calcium carbonate2 h wt.
Aqueous crystalline trehalose50 h wt.
"; G-HESPERIDINE®", α-glycosylphosphatidyl2 h wt.
Powder containing associat trehalose and components uterine salt solution obtained by the method of example 8100 h wt.
Coloring agents and perfumesThe desired number

Because the product contains trehalose and magnesium, it has a satisfactory ability to retain moisture and retain heat and is suitable as a means of skin care and whitening tools. The product can be used with dilution in 1000-10000 times with lukewarm water for bathing. The product has the advantage of reducing the amount of soap precipitation, cereals. Further, the product can also be used as a cleaning lotions and lotions by dilution.

Example 22

Soy sauce

Soy sauce was prepared in accordance with the following composition. Aqueous crystalline trehalose added to "TOKUSEN-MARUDAIZU-GENEN-SHOYU", soy sauce, low salt, sold by Kikkoman Co. Ltd., Chiba, Japan, which does not contain trehalose, to obtain a concentration of 10% (wt./wt.) and dissolved to obtain soy sauce.

Because the product contains a relatively large amount of trehalose in comparison with soy sauce that does not contain trehalose, it inhibits the formation of plaque, particularly USAID is of the compounds, containing magnesium ions, while cooking food with boiling and dishes cooked in a pot. Although the product is a soy sauce, low salt, it can preferably be used for seasoning food, subjected to boiling for daily meals, fried foods, soups and to preserve the taste of food as conventional products.

Example 23

"Miso" (soybean paste)

"Miso" (soybean paste) was prepared in accordance with the following composition. Aqueous crystalline trehalose added to "TAKEYA-MISO-SHIO-HIKAEME", "miso", low salt, sold by Takeya Miso Co. Ltd., Nagano, Japan, which does not contain trehalose, to obtain a concentration of 8% (wt./wt.) and mixed to homogeneity to obtain a "miso".

Because the product contains a relatively large amount of trehalose compared with "miso", not including trehalose, it inhibits the formation of plaque, in particular for the deposition of compounds containing magnesium ions, while cooking food with boiling and dishes cooked in a pot. Although the product is a "miso" with low salt content, it can preferably be used for seasoning food, subjected to boiling for daily meals, heat is the major food soups and to preserve the taste of food as conventional products.

Example 24

Mineral water

Mineral water was prepared in accordance with the following procedure. Aqueous crystalline trehalose was added to the soil (spring) water, which was pumped into the mountain, to obtain a concentration of 0.5% (wt./wt.) and diluted. The resulting solution was sterilized by filtration through a membrane filter. Sterilized bottles filled with filtrate for making poured in bottles of mineral water. The content of basic compounds containing metal ions, mineral water, reached 40.9 ppm calcium, 12,5 ppm sodium and 11.6 ppm magnesium.

Because the product includes trehalose, formed the associates of trehalose and compounds containing metal ions. The product doesn't dim during storage for a long period, because it improves the solubility of associates. This mineral water is of high quality, including appropriate amounts of minerals, which gives a pleasant sensation of taste and good thirst quencher.

Example 25

Isotonic drink

Isotonic drink was prepared by mixing and dissolving the components in accordance with the following composition.

Isomerized with the har (fructose-glucose syrup) 2 h wt.
Aqueous crystalline trehalose3 h wt.
Maltitol3 h wt.
Lemon juice1 h wt.
L-ascorbic acid0,1 h wt.
Citric acid0,06 h wt.
Sodium citrate0,03 h wt.
Sodium chloride0,05 h wt.
Monosodium phosphate0,05 h wt.
Calcium lactate0,015 h wt.
Magnesium chloride0,01 h wt.
Coloring agents and flavoringAn appropriate number
Water90,685 h wt.

Since the product comprises trehalose and maltitol, formed the associates of trehalose and compounds containing metal ions. The product doesn't dim during storage for a long period, because it improves the solubility of the calcium salts of organic acids. This isotonic drink is high quality with a pleasant taste sensations.

Example 26

Dried "wakame" (brown seaweed)

Aqueous crystalline trehalose was added to sea water to obtain a concentration of 8% (wt./wt.) and dissolved by heating. After otelia the Oia "wakame" in the solution, the temperature of which was maintained at a level 80-85°S, it was dried to obtain a dried "wakame".

Because trehalose and components uterine saline contained in sea water, forming associat on the surface "wakame", the hygroscopicity of the product after drying is reduced, and the product does not show the stickiness caused by the adsorption of moisture during storage. The product can preferably be used as the material for lettuce and ingredients for soup "miso", and he hardly forms a scum during cooking. Further, the product can be used as food products such as confectionery and food materials.

Example 27

Dried kombu (seaweed)

Aqueous crystalline trehalose was added to sea water to obtain a concentration of 6% (wt./wt.) and was dissolved at ambient temperature. After impregnation of kombu in the solution, it was dried in sunlight for the manufacture of dried kombu.

Because trehalose and components uterine saline contained in sea water, form associates, the hygroscopicity of the product after drying was reduced, and the product does not show the stickiness caused by the adsorption of moisture during storage. The product can preferably be used as kombu soup, and it hardly forms a scum when p is the production. The product is a useful food material for "koujima" (pickled foods seaweed), "kuzumaki" (rolled seaweed) and kabocha" (tea made from seaweed), and it can be used as food products such as confectionery products and food materials.

Example 28

Soap

Soap was obtained by mixing the components until smooth in accordance with the following composition.

Pure soap derived from a mixture of beef fat and palm oil at a mass ratio of 2:1, by way of saponification-vysalivaniya80 h wt.
Aqueous crystalline trehalose10 h wt.
Maltitol9 h wt.
2-glucoside L-ascorbic acid0.5 h wt.
Sucrose0.5 h wt.
"KANKO-No. 2010,0001 h wt.
FlavoringsAn appropriate number

The product is a high quality soap with a satisfactory property foaming and washing ability. The precipitation of sparingly soluble salts, in particular compounds containing magnesium ions, which have the property of deposition by dissolving soap in the harsh conditions of the water, including compounds containing metal ions can be suppressed using the product. As a result, the product reduces the formation of soap flakes, and its ability to lather and wash-ability is unlikely to deteriorate. Further, the product can preferably be used as soap, which prevents odor and itching of the body, because it inhibits the formation of volatile aldehydes and/or the decomposition of fatty acids that originate from sweat fluid, dirt and sebum.

Example 29

Toothpaste

A toothpaste was prepared by mixing the components according to the following composition.

Calcium phosphate (CaHPO4)48 h wt.
Sodium lauryl sulfate1.5 h wt.
Glycerin25 h wt.
Laurate of polyoxyethylenesorbitan0.5 h wt.
Aqueous crystalline trehalose10 h wt.
Maltitol10 h wt.
Preservatives0,05 h wt.
Water13 h wt.

Improving the unpleasant taste of the product, and it has a satisfactory availability without reducing the cleaning power of detergents. Because trehalose and maltitol, which is errada in the product, form associates with compounds containing metal ions, the product has the capacity to suppress the adhesion of Tartar and plaque, which are formed by compounds containing calcium ions and magnesium, and contribute to their dissolution. Therefore, the product has a satisfactory capacity to brush your teeth with applying it on your toothbrush.

Example 30

The broth for Nabemono" (Japanese dish cooked in a pot)

The broth for Nabemono" (Japanese dish cooked in a pot) was prepared in accordance with the following procedure. To 2.4 h masses. "Udon soup", a commercially available powdered soup "Udon" (Japanese noodles), sold by the company Hagashimary Shoyu Co., Ltd., Hyogo, Japan, which does not include trehalose, and 10 h of the masses. aqueous crystalline trehalose was mixed into 90 h of the masses. water and dissolved to prepare the broth for Nabemono" (Japanese dish cooked in a pot).

Because the product contains a relatively large amount of trehalose, it inhibits the formation of scale, in particular the deposition of compounds containing magnesium ions, from meat and vegetables while cooking "Nabemono" (Japanese dishes, cooking utensils). The product also suppresses the elution of compounds containing magnesium ions, from food materials. The product can preferably be used for cooking, boil the's food "Nabemono" (Japanese dishes, cooking utensils), everyday food, soup and to improve the taste of food.

Industrial applicability

As described above, the present invention has revealed that trehalose and maltitol formed associates with compounds containing metal ions, or components uterine saline, direct interaction in the presence of compounds containing metal ions, or components uterine saline. As associates of the present invention have a reduced hygroscopicity, high solubility and low reactivity against oxidation and recovery, they are also very useful because of the possibility of industrial manipulation compared with conventional compounds containing metal ions or components uterine saline. Associates of the present invention can preferably be used in various fields, in which compounds containing metal ions or components uterine saline, used as materials, ingredients and products, for example in food products (including beverages), agricultural and marine products, beauty products, medicines, consumer goods, chemical p is the itsindustry and industrial sectors for the production of materials and ingredients, used in these areas.

The present invention having the above positive features and effects, represents a significant important invention makes a great contribution in this area.

1. Crystalline monohydrate of associate containing trehalose, calcium chloride and water in a molar ratio of 1:1:1, where the specified crystal associat in the analysis of powdered material by x-ray diffraction is the main diffraction angles (2θ) 9,02°, 17,98° and 21,90°.

2. Anhydrous crystal of associate containing trehalose and calcium chloride in a molar ratio of 1:2, where the specified crystal associat in the analysis of powdered material by x-ray diffraction is the main diffraction angles (2θ) 12,66°, 21,02° and 25,48°.



 

Same patents:

FIELD: biotechnology, biochemistry, enzymes.

SUBSTANCE: invention relates to a method for preparing 4-O-β-D-galactopyranosyl-D-xylose used for in vivo assay of lactase activity in human intestine. Method involves interaction of D-xylose with β-D-galactopyranoside for 2-48 h in a solution at pH 5-9, at temperature that changes from the mixture freezing point to 45°C, and the following addition of 10-1000 U of β-D-galactosidase per 1 g of β-D-galactopyranoside. Reaction is terminated by inactivation of enzyme followed by isolation and crystallization of fraction containing 4-O-β-D-galactopyranosyl-D-xylose in the crystallization mixture chosen from acetone/methanol = (5-1):(20-1) and acetone/water = (5-1):(20-1). Invention provides increasing content of 4-O-β-D-galactopyranosyl-D-xylose in the final mixture.

EFFECT: improved preparing method.

42 cl, 2 tbl, 9 ex

The invention relates to food industry, namely breast

The invention relates to new derivatives of 3-deoxyanthocyanins formula I, in which X denotes a radical of the formula V or a radical of the formula C; Y denotes a radical of the formula D; R1, R3, R5, R7, R8, R10, R13- the same or different, represent each a linear or branched CNS radical with 1-6 C-atoms or the radical-OSO-3; R2, R4, R6, R9, R11- the same or different, represent each a hydrogen atom, a linear or branched CNS radical with 1-6 C-atoms or the radical-OSO-3, R12means a hydroxyl radical or a radical-OSO-3however , provided that at least one of the substituents R2or R4or R6or R9or R11denotes a hydrogen atom; in the form of pharmaceutically acceptable salts and the corresponding acids

The invention relates to biochemistry, namely to obtain biologically active substances with antiviral activity

The invention relates to pharmacology, in particular ORGANOMETALLIC compounds possessing biological activity, which can find application in drug development for the prevention and treatment of coronary heart disease

FIELD: cosmetics, pharmacy.

SUBSTANCE: invention relates to associates containing trehalose and calcium chloride as a crystalline monohydrate comprising above said components and water in the molar ratio = 1:1:1, or as anhydrous crystal containing above said components in the molar ratio = 1:2. Indicated crystals of two species of associates of trehalose and calcium chloride can be used in different branches in production of foodstuffs, cosmetic agents and others.

EFFECT: valuable properties of compounds.

3 cl, 20 tbl, 15 dwg, 30 ex

FIELD: chemistry.

SUBSTANCE: invention relates to a method of obtaining oxycobalamine - catalyst for catalytic therapy of malignant growths through electrochemical reduction of Co3+ to Co2+ in cyanocobalamine in a membrane cell on a titanium cathode in galvanostatic mode at current density 5.0-15.0 A/dm2 in an aqueous solution of hydrochloric acid and temperature 28-40°C, with subsequent oxidation of the latter with atmospheric oxygen with formation of oxycobalamine and separation of the end product through precipitation from a water-acetone mixture while cooling.

EFFECT: increased output of desired product up to 80,0-90,0%.

4 ex, 2 tbl

FIELD: medicine.

SUBSTANCE: invention refers to cobalamin derivatives of the formula , where Rb means spacer chelating group of the formula , where n is 2, 3 or 4; Rc, Rd, Re and RR denote hydrogen, and X is cyano; or where Rd indicates spacer chelating group of the formula , where n is 3; Rb, Rc, Re, and RR denote hydrogen, and X is cyano, or where Rb indicates spacer chelating group of the formula , where n is equal to 2; Rc, Rd, Re and RR denote hydrogen, and X is cyano. These compounds are accumulated to a much lesser extent in blood and non-malignant organs such as kidneys and liver, compared with the rate of accumulation in neoplastic tissues, and moreover they are quickly removed from blood. The invention also refers to injectable pharmaceutical composition for diagnosis of tumors including cobalamin derivative carrying a radioactive metal atom. In addition, the invention refers to diagnosis of tumors in mammals, including (a) compliance vitamin B12-free diet for mammals predisposed to neoplastic disease, (b) subsequent administration of cobalamin derivative carrying a radioactive metal atom. The invention also refers to use of cobalamin derivative carrying a radioactive metal atom, in method of diagnosis of tumors in mammals and to obtain pharmaceutical composition designed for use in method of diagnosis of neoplastic diseases in mammals.

EFFECT: improvement of composition application.

13 cl, 2 tbl, 6 dwg, 35 ex

FIELD: chemistry.

SUBSTANCE: water-salt solution of vitamin B12 with pH 5.3±0.3 is prepared, for which the solution of vitamin B12 with pharmacopoeial purity is put into a measuring flask and saturated with ammonium sulphate solution. Vitamin B12 is extracted with chemically pure isopropyl alcohol, for which the obtained vitamin B12 solution is mixed with isopropyl alcohol in volume ratio of the water-salt solution of vitamin B12 to the organic phase is equal to 20:1. Extraction is carried out in 4-5 minutes until interphase equilibrium is established. After stratification of the system, the aqueous phase is separated from the orgnaic phase and analysed through UV spectrophotometry. A calibration curve of optical density of the water-salt solution versus concentration of vitamin B12 is plotted. Optical density of the water-salt solution is measured in a UV spectrophotometre in a quartz cell 1=1 cm with wavelength 361 nm. Concentration of vitamin B12 in the aqueous phase is determined from the calibration curve.

EFFECT: complete one-time extraction of vitamin B12 from an aqueous solution, reduced labour input, high sensitivity and safety of analysis.

1 ex, 1 tbl

FIELD: chemistry.

SUBSTANCE: method involves preparation of a water-salt solution of vitamin B12 through dissolution thereof in a saturated solution of a salting-out agent, extraction and analysis of equilibrium aqueous phase, wherein the extraction agent used is aqueous solution of polyethylene glycol with molar mass of 5000 g/mol, the salting-out agent used is ammonium sulphate. The water-salt mixture of vitamin B12 with pH 5.3±0.3 is prepared by putting a sample of vitamin B12 with pharmacopoeial purity, taken on analytical balances, into a measuring flask and bringing to the mark with a saturated solution of ammonium sulphate. Vitamin B12 is then extracted with aqueous solution of polyethylene glycol with concentration of 10-4 mol/dm3 by adding to the obtained vitamin B12 solution aqueous solution of polyethylene glycol in volume ratio of the water-salt solution of vitamin B12 to the polymer solution equal to 10:1 and then extracted for 6-7 minutes until establishment of inter-phase equilibrium. For stratification of the system, the solution is put into a laboratory centrifuge for 5-7 minutes rotating at 1000 rpm. The water-salt phase is then separated from the water-organic phase and then analysed via UV spectrophotometry. A calibration curve of optical density of the water-salt solution versus concentration of vitamin B12 is then plotted. Optical density of the water-salt solution is then measured on a UV spectrophotometre in a quartz cell with thickness of the light-absorbing layer equal to 1 cm and wavelength of 360 nm. Concentration of vitamin B12 in the aqueous solution is then found using the calibration curve.

EFFECT: high distribution and concentration coefficients, faster and safer process.

1 ex, 1 tbl

FIELD: chemistry.

SUBSTANCE: water-salt mixture of vitamins B1 and B12 is prepared, for which the analysed vitamin solutions with pharmacopoeial purity are put into a measuring flask, while stirring, brought to the mark using a saturation solution of a salting-out agent in form of ammonium sulphate. The vitamins are extracted with chemically pure isopropyl alcohol by adding to the obtained water-salt mixture of vitamins isopropyl alcohol in volume ratio of the water-salt mixture of vitamins to isopropyl alcohol equal to 20:1, extracted for 5-7 minutes and after stratification of the system, the aqueous phase is separated from the organic phase. Optical density of the water-salt mixture is measured on a UV spectrophotometre at wavelengths 246 nm and 360 nm (thickness of the light-absorbing layer equal to 1 cm). Molar concentration of vitamins B1 (CB1) and B12 (CB12) is calculated using equations: CB1=6.17·10-5·A246-4.21·10-5·A360 CB12=3.36·10-5·A360, where A426 and A360 are optical density of the solution of mixtures of vitamins B1 and B12 at 246 nm and 360 nm, respectively.

EFFECT: rapid analysis and possibility of almost complete extraction of vitamin B12 from a water-salt mixture during single extraction.

1 ex

FIELD: biochemistry.

SUBSTANCE: invention refers to derivatives of 3'-ethynilcytidine represented by the formula (1); (where X is hydrogen atom, alkylcarbonyl group where alkyl fragment is non-branched or branched C1-C6alkyl group which can include as substitute(s) mono-or di-substituted with non-branched or branched C1-C6alkyl group aminogroup or alkoxycarbonyl group where alkoxy-fragment is a branched or non-branched C1-C6 alkoxygroup; One of Y and Z is a hydrogen atom or group (R1)(R2)(R3)Si-, and another is a group (R4)(R5)(R6)Si-; and each R1, R2, R3, R4, R5 and R6 that may be similar or different are non-branched or branched C1-C10 alkyl group or C6-C14aryl group) or to its salts. The invention also refers to the derivative of 3'-ethynilcytidine chosen out of compounds (1)-(17), to the pharmaceutical composition, to oncologic drug, to oral oncologic drug, to the use of derivative of 3'-ethynilcytidine, and to the method of tumor treatment.

EFFECT: new biologically active compounds with antitumor activity.

14 cl, 18 ex, 12 tbl

FIELD: medicine.

SUBSTANCE: invention relates to pharmacology, and describes a method of producing coordination compounds of oligogalacturonic acid with biogenic metals (II). Method involves production of pectin-containing preparation of oligogalacturonic acid with polymerisation degree equal to no less than 10, subjected to reaction with metal salt (II): copper, cobalt, iron, zinc, manganese, magnesium in aqueous medium at neutral pH, exposing reaction mixture at room temperature, neutralisation of formed acid, water flushing, centrifugation and drying precipitate. According to invention to 1 l of 5.0·10-3 M aqueous solution of purified oligogalacturonic acid while stirring is added first 2 M aqueous solution of ammonia hydrate to pH 8, then 1 l of 4.5·10-2 M aqueous solution of metal acetate (II); formed gel-like precipitate is treated with 96 % ethanol at volume ratio of 1:2, held for 2 hours.

EFFECT: invention ensures delivery of biogenic metals (II), as well as removal of heavy metal cations (II) from body.

1 cl, 8 tbl, 4 dwg, 6 ex

FIELD: biotechnology, biochemistry, enzymes.

SUBSTANCE: invention relates to a method for preparing 4-O-β-D-galactopyranosyl-D-xylose used for in vivo assay of lactase activity in human intestine. Method involves interaction of D-xylose with β-D-galactopyranoside for 2-48 h in a solution at pH 5-9, at temperature that changes from the mixture freezing point to 45°C, and the following addition of 10-1000 U of β-D-galactosidase per 1 g of β-D-galactopyranoside. Reaction is terminated by inactivation of enzyme followed by isolation and crystallization of fraction containing 4-O-β-D-galactopyranosyl-D-xylose in the crystallization mixture chosen from acetone/methanol = (5-1):(20-1) and acetone/water = (5-1):(20-1). Invention provides increasing content of 4-O-β-D-galactopyranosyl-D-xylose in the final mixture.

EFFECT: improved preparing method.

42 cl, 2 tbl, 9 ex

FIELD: cosmetics, pharmacy.

SUBSTANCE: invention relates to associates containing trehalose and calcium chloride as a crystalline monohydrate comprising above said components and water in the molar ratio = 1:1:1, or as anhydrous crystal containing above said components in the molar ratio = 1:2. Indicated crystals of two species of associates of trehalose and calcium chloride can be used in different branches in production of foodstuffs, cosmetic agents and others.

EFFECT: valuable properties of compounds.

3 cl, 20 tbl, 15 dwg, 30 ex

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