Method for predicting chronic pyelonephritis in children

FIELD: medicine, pediatrics, immunology.

SUBSTANCE: in patients' peripheral blood one should detect the expression level of CD95 lymphocytes markers due to indirect immunofluorescence technique and at the content of CD95 markers being above 21.31% one should detect chronic flow of pyelonephritis. The method is simple and of high information value. It enables to evaluate quantitative marker of affected mechanisms of apoptosis at chronic pyelonephritis quickly and objectively for 2.5 h after blood sampling, moreover, it is of high information value for differential diagnostics of chronic pyelonephritis and provides rational complex of therapeutic procedures in due time.

EFFECT: higher efficiency of diagnostics.

1 ex, 1 tbl


The invention relates to medicine, in particular to Pediatrics, immunology. It can be used in the clinic for the diagnosis of chronic pyelonephritis (CP) in children.

A known method for the diagnosis of CP on clinical grounds according to which CP differentiate from cystitis, glomerulonephritis, hereditary nephritis and other diseases (Vozianov A.F., maidannyk VG, a Prominent VG, Bagdasarova IV Basics of Nephrology children. - K.: Book plus, 2002. - S-91).

A known method for the diagnosis of pyelonephritis to determine the level of cytokines, which is selected as the closest analogue. This method comprises detecting increased levels of cytokines (tumor necrosis factor-alpha, transforming growth factor-beta 1) in plasma by ELISA (Khvorostov, I.N., Smirnov IE, Zorkin, S.N., Kucherenko A.G. cytokines with obstructive uropathy in children // Pediatrics. - Annex 1: proceedings of the X Congress of pediatricians of Russia "Ways of increasing the effectiveness of health care for children". - 2005. - V.4. - S). The disadvantage of this method is its high cost.

The aim of the invention is to improve the accuracy, efficiency and objectification of the differential diagnosis of chronic pyelonephritis, which will ensure the speed of diagnosis, economical, and timely the military and rational realization of a complex of therapeutic measures.

The essence of the proposed diagnostic method is that along with clinical data and urinalysis determine the level markers CD95 lymphocytes of peripheral blood of a child by indirect immunofluorescence using monoclonal antibodies and content markers CD95 more 21,31% of diagnosed chronic pyelonephritis.

CD95 (weight fasr) refers to the receptor protein family factors tumor necrosis (TNF - a. The main function of the system Fas-receptor-Fas-ligand (FasL) - induced apoptosis in the cells. When the binding of the ligand to the receptor occurs oligomerization of the cytoplasmic proteins, resulting activated apoptosisinducing protease (caspase-8) and develop characteristic for apoptosis processes. In addition to genetically determined mediators of apoptosis, the cell may be exposed to compounds that regulate this process, such as cytokines (interleukins 2, 4, 10)circulating in the blood or associated with biomembranes. In infectious processes development of apoptosis depends on specific factors secreted by microorganisms (superantigen-exotoxins often act as inductors activation of apoptosis). The destruction of the cells of the tubular epithelium, along with necrosis, can occur by apoptosis. In the final stages of inflammation, adopt the zu has an important role, because in this period the elimination of activated cells of the immune system, performed their functions. Possible negative role of apoptosis in the development of secondary immunodeficiency in patients with chronic pyelonephritis.

The method is as follows: samples (4-5 ml) of heparinized blood taken on an empty stomach in children, isolated lymphocytes on the gradient ficoll-urografin, then 1-0,1 million cells in a volume of 50 μl contribute to the wells of 96-well round-bottom of the tablet. To the cells, add 5 µl of the tested monoclonal antibodies (CD95) and incubated for 30-45 min at +4°C. Then add 150 μl of Hanks solution and centrifuged 5 min at 200 g. After removal of the supernatant contribute 50 μl of Hanks solution, the cells are suspended, add 150 μl of Hanks solution and centrifuged 5 min at 200 g. To the precipitate washed cells add 50 μl of F(ab˜)2fragments of sheep antibody to mouse Ig labeled with FITZ and diluted 1:100 (for cultivation using the Hanks solution containing 0.5% gelatin and 0.1% sodium azide). Cells are suspended and incubated for 30 min at +4°C, the cells are then washed 2 times as described above. Stained cells analyzed by fluorescence microscope. Cells are suspended, transferred to a glass slide, covered with a cover glass, and looking at the drug under immersion with increasing (#x000D7; 3; lens ×90). The number antigenpositive cells is defined as the percent of fluorescent cells while browsing 200 lymphocytes minus % fluorescent cells observed in the preparation of the negative control. As a negative control, using drugs, prepared similarly, but without the processing of monoclonal antibodies. When the expression of markers CD95 more 21,31% lymphocytes establish chronic pyelonephritis.


Girl Days, 5 years. A year ago she was hospitalized for acute pyelonephritis; noted the symptoms of intoxication, abdominal pain syndrome, bladder syndrome, is represented by leukocyturia, bacteriuria. Two days ago after a short hypothermia increased body temperature up to 38,50°C, pains in the stomach, in the lumbar region. When admitted to hospital, the condition of moderate temperature of 38.5°, pale skin, periorbital cyanosis. Swelling and pastoznost tissues no. Vesicular breathing in lungs. NPV 20 in 1 min. heart sounds satisfactory sonority. Pulse 122 1 min, BP 100/60 mm Hg Abdomen is soft, painful to palpation in the area of the right flank. Symptom of Pasternacki to the right is positive. Urination acadeny up to 10 times per day, painless. In General, the analysis of blood stoichiometric who think leukocytosis, increased ESR. Biochemical blood analysis: total protein, urea, creatinine within normal limits, C-reactive protein - negative. In the General analysis of urine - leukocyturia, oxaluria. Urine analysis according to Nechyporenko: leukocytes - 25.500 1 ml (neutrophils 82%, lymphocytes 18%), erythrocytes - 1000 in 1 ml Bacteriological examination of urine: 100000 E. coli CFU in 1 ml sensitive to Ceftriaxone, ceftazidime, gentamicin, norfloxacin. Reduced antichristianity the ability of urine to the oxalate of calcium. According to the results of research on clearance of endogenous creatinine, urine analysis zimnitsky - kidney function is preserved. Ultrasound of the kidneys and bladder: the heterogeneity of the Central echo complex due hyperechoic signals, broken relationship parenchyma and Cup-pelvis system right, echo signs of inflammation of the right kidney. Excretory urography: allocation of the contrast agent is not broken, there is a smoothness of Vernikov right kidney.

The level of expression of markers CD95 the peripheral blood lymphocytes of 25.00%.

Diagnosis. Chronic secondary (dysmetabolic) pyelonephritis, relapsing course, active stage. Kidney function is preserved.

Data show that the level markers CD95 of peripheral blood lymphocytes may serve as an additional criterion in the differential diagnosis of CP

A method for the diagnosis of chronic pyelonephritis in children according to the level of expression of markers CD95 by peripheral blood lymphocytes was tested on 38 children with a prior diagnosis of chronic pyelonephritis set on the basis of clinical signs (main group). The comparison group consisted of 14 children with a diagnosis of acute pyelonephritis, the control group of 30 children from the first group of health with normal blood and urine tests. All of the respondents had performed blood sampling and determination of the level of expression of markers CD95 by peripheral blood lymphocytes listed above.

The results of the study are presented in the table. For processing the received data, and statistical analysis in the studied groups was used for the statistical processing of information "STATGRAPHICS PLUS". To calculate the average and the average error (M±m). Comparison of study groups conducted in pairs, using the parametric methods because the study sample could be approximated by a normal distribution. The comparison was performed using t-test (t) (comparison means) and Fisher (comparison of mean square deviations). The confidence level was assessed value R. Differences when the comparison was considered significant with a probability of more than 95% if < 0,05.


CD95 (%)

M±m (min, max)
Chronic pyelonephritis25,45±2,07*
Acute pyelonephritis14,20±1,38*
(n=30)(of 7.36-8,64)
Note: * - p<0,05 - significance of differences in the groups in the comparison.

In healthy children markers CD95 Express no more than 8,64% of peripheral blood lymphocytes in children with acute pyelonephritis - no more 16,96%.

The obtained data showed that in CP level markers CD95 of peripheral blood lymphocytes was significantly increased compared with levels in children with SOS and control group, therefore, the level of expression of CD95 molecules by peripheral blood lymphocytes can be considered as a highly sensitive marker in CP.

Using the proposed diagnostic method has the following advantages:

1) high information content for the differential diagnosis of CP;

2) is informative method of quantification brand is and violations of apoptosis mechanisms in CP dynamics;

3) rapid diagnostics;

4) ensure timely and efficient implementation of the complex of therapeutic measures.

A method for the diagnosis of chronic pyelonephritis, including the study of the peripheral blood, characterized in that to determine the level of expression of lymphocyte markers CD95 by indirect immunofluorescence using monoclonal antibodies and when the content in the peripheral blood markers CD95 more 21,31% believe the presence of chronic pyelonephritis confirmed.


Same patents:

FIELD: clinical diagnosis, in particular in vitro determination of skin tuberculin high-grade sensitivity.

SUBSTANCE: skin tuberculin high-grade sensitivity is determined based on alteration of fluorescent intensity of common leucocytal CD45 antigen and isoforms thereof CD45RA and CD45RO in test probe (after incubation of peripheral blood with 2TE tuberculin solution) in contrast to control probe with physiological solution by using monoclonal antibodies labeled with fluorescein isothiocyanate (FITC) and laser flow cytofluorometry.

EFFECT: new method for in vitro determination of skin tuberculin high-grade sensitivity.

1 tbl, 4 ex

FIELD: biotechnology, immunology.

SUBSTANCE: invention proposes hybridomas producing monoclonal antibodies showing specificity to human epiregulin. Invention discloses monoclonal antibody recognizing specifically human epiregulin with the sensitivity limit 10 pg/ml. Also, invention describes methods for specific detection of human epiregulin in a sample in vitro and a method for detection of cells expressing human epiregulin in extracellular fluid in vitro by using monoclonal antibody to human epiregulin. Invention provides a simple and highly sensitive method for detection of human epiregulin that can be used in diagnosis of human epiregulin-expressing tumors.

EFFECT: valuable biological and medicinal properties of antibody and hybridoma.

8 cl, 7 dwg, 8 ex

FIELD: medicine, neonatology.

SUBSTANCE: in umbilical cord blood at the moment of a child's birth one should detect the ratio of the parameters for relative content of CD3+HLA-DR+ against CD3+ lymphocytes and at its value being equal to 8.1% or above it one should diagnose perinatal hypoxic CNS lesion at accuracy of 89.28%. Application of the present method enables to diagnose perinatal hypoxic CNS lesion in mature neonatals in gestosis-suffering women at earlier terms.

EFFECT: higher accuracy of diagnostics.

3 ex, 1 tbl

FIELD: immunology, biotechnology.

SUBSTANCE: invention relates to antibodies showing specificity to anomalous processed form of human tau protein that differs by conformation from the normal tau protein and doesn't bind with normal tau protein. Also, invention relates to conformational distinctive tau proteins ("tauones") and diagnostic and therapeutic aspects related to Alzheimer's disease and related taupathies. Proposed antibodies are produced by hybridomas DC-11 or Dc-11/1 deposited in ECACC at numbers 00082215 and 00082216. Also, invention described truncated forms of human tau protein that are truncated by N- and/or C-end and comprise amino acid residues from amino acid 300 to amino acid 400 in the longest isoform of human tau protein (441 amino acids residues). Above mentioned truncated forms of human tau protein can be recognized specifically by antibodies described above. Also, invention describes a method for assay of truncated forms of tau protein in a patient biological sample using a set comprising a proposed antibody and suitable container. Using the proposed invention provides a suitable target for medicinal preparations with early therapeutic effect used in Alzheimer's disease and other taupathies.

EFFECT: valuable medicinal properties of proteins.

11 cl, 15 dwg, 10 ex

FIELD: medicine, laboratory diagnostics, pediatrics, neonatology.

SUBSTANCE: in neonatals born in mothers with endemic goiter it is necessary to detect the content of CD25+ and HLA-DR+ lymphocytes in peripheral venous blood on the 5th d of their life and at the value of the first parameter being either equal or below 6.9% and the second parameter being either equal or below 16.5% one should predict the onset of infectious-inflammatory diseases in the course of neonatal period.

EFFECT: higher accuracy, sensitivity and specificity of prediction.

3 ex, 1 tbl

FIELD: medicine, analytical immunology.

SUBSTANCE: invention relates to a set of reagents used in quantitative determination of secretory immunoglobulin A (sIgA) that provides assaying status of topical immunity and immunodeficient states in carrying out analysis of serum and secrets of human body. Proposed set comprises a plate with immobilized monoclonal antibodies, five calibrating samples containing 0; 1.0; 5.0; 10.0 or 20.0 mcg of sIgA/ml, conjugate of murine monoclonal antibodies with horse radish peroxidase and a reagent for carrying out the enzymatic reaction. Murine monoclonal antibodies produced by hybrid strain of animal Mus musculus L., № PKKK (P) 676D cultured cells, are immobilized on a carrier. Conjugate comprises murine monoclonal antibodies produced by another strain - Mus musculus L., № PKKK (P) 677D. The advantage of invention involves enhancing sensitivity in assay of sIgA.

EFFECT: improved assay method.

3 tbl, 5 ex

FIELD: medicine.

SUBSTANCE: method involves determining availability of antibodies to basic myelin protein at the first and the third week after burn, by applying immunoenzyme analysis approach. The third week values belonging to the range of 0.1-0.19 ODU, vegetovascular dystonia syndrome is predicted to develop. The values being within the 0.2-0.274 ODU limits, disseminated cerebral microsymptoms are to be predicted. The values being within the 0.275-0.3 ODU limits, focal symptom manifestations syndrome is to be predicted.

EFFECT: high accuracy in predicting cerebral disorders severity degree.

FIELD: medicine.

SUBSTANCE: method involves determining relative content of CD16+ monocytes in monocytic gate in peripheral venous blood of barren woman. The value being equal to or less than 45%, external genital endometriosis diagnosis is to be set.

EFFECT: high accuracy, specificity and sensitivity of diagnosis method.

1 tbl

FIELD: medicine; microbiology.

SUBSTANCE: method an be used for specific identification and evaluation of potential ability of non-cultivated forms of vibrio cholerae O1 and O139 serous groups to reversion, which vibrios take place in water solutions. Method is based upon preliminary preparation of bacterial suspension of non-cultivated forms of vibrio cholerae O1 and O139 serous groups and bacterial suspension of reference culture of Vibrio Cholerae O1 and O139 serous groups. The working dilution of bacterial suspensions and monoclonal antibodies conjugate is determined and specific monoclonal antibodies are selected for conservative and labile lactose-peptone medium epitopes followed by dot-immune-enzyme analysis or solid phase immune-enzyme analysis reaction. Then appearance of color point or optical density, being higher than 0,2-0,65, is registered and conclusion is made on presence of non-cultivated forms of Vibrio Cholerae O1 and O139 serous groups in sample and their potential reversion ability. Preliminary preparation of non-cultivated forms is performed due to increase in their concentration up to 106-107 mxkl/ml followed by inactivation. For the purpose 20 mkl of 4% sterile gentamicine solution is added to 1 ml of microbe suspension followed by subsequent cooling of samples down to 3-4 deg C during 2 hours. Bacterial suspension of control culture Vibrio Cholerae O1 or Vibrio Cholerae O31 is made from 18-hours culture, grown onto Martin's agar. After that the suspension is used for preparation of bacterial suspension in physiological solution, pH 7,6 till final concentration of 107 mxkl/ml. Method uses set of specific monoclonal antibodies, got to different epitopes of O-antigen of lactose-peptone medium cultures of Vibrio Cholerae O1 and O139 serous groups: 3A9, D11, 3E4, 4H5, D8D6, C3B4, F8G12, F11E5.

EFFECT: ability of getting express-answer at low concentration of tested cells.

5 cl, 2 tbl

FIELD: medicine, laboratory diagnostics.

SUBSTANCE: it is necessary to study saliva due to carrying out immunoenzymatic assay by applying mycobacterial antigen specifically binding with human secretory immunoglobulin A only, and specific monoclonal antibodies directed against human secretory immunoglobulin A. The present method is considered to be of high efficiency, noninvasive, simple in application, of high information value, specificity and sensitivity to tuberculosis.

EFFECT: higher accuracy of diagnostics.

3 ex, 2 tbl

FIELD: medicine.

SUBSTANCE: method involves developing combined evaluation of objective and subjective factors by applying testing system with permissible deviations from reference values being specified. Medical service quality standard is taken for 1. A medical service is acknowledged to be of quality, if score sum of all partial estimates divided by their number is equal to 0.8.

EFFECT: enhanced effectiveness in controlling relations in medical care system.

4 tbl

FIELD: medical equipment.

SUBSTANCE: detector for noninvasive distant reception of self-radiation of person has person's self-radiation receiver. Receiver has single-crystal Z-cut quartz plate. Both sides of plate are covered with silver; coatings are electrically connected with electromagnet signals' amplifier. Amplifier is placed in case made of material being non-penetrable for electromagnet radiation. Quartz plate is disposed in cavity of contraction reflector of electromagnet radiation reflector made of non-ferrous material. Internal surface of reflector is coated with silver. Axis of reflection of reflector passes center of quartz plate and it is perpendicular to surface of plate. Quartz plate is mounted for oscillation along axis of symmetry of reflector. Electromagnet amplifier's case is attached directly to back edge of case of contraction reflector.

EFFECT: simplified procedure of diagnosing; improved truth of diagnosis.

3 cl, 7 dwg

FIELD: medicine, surgery.

SUBSTANCE: it is necessary to carry out bioimpedansometry of the upper (Z1) and the lower (Z2) splenic poles to compare the data obtained and if Z1 is above or equal to Z2 one should sate upon splenic traumatic lesion. The innovation enables to carry out diagnostics of splenic traumatic lesion objectively and reliably.

EFFECT: higher accuracy of diagnostics.

1 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: the present innovation deals with predicting the state of living organisms. It is necessary to carry out the freezing and microscopic testing human and animal biological liquids at -5°C for the presence of crystalline structures. The crystals developed for making the diagnosis of an organism's state should be tested according to the following diagnostic parameters: the shape of crystals, and/or the structure of crystals' edges, and/or the color of crystals, and/or crystals' transparency. As biological liquids one should study blood and/or urine, and/or saliva, and/or perspiration, and/or purulent excreta, and/or lacrimal liquid and/or animal excreta, for example, mucus.

EFFECT: higher accuracy of diagnostics.

2 cl, 5 dwg, 1 ex

FIELD: medicine, pulmonology.

SUBSTANCE: a patient's blood should be tested to detect the content of substance of low and middle molecular mass (SLMMM) in arterial and venous blood. According to the ratio SLMMMarterial plasma/SLMMMvenous plasma one should detect the coefficient of metabolic pulmonary dysfunction (CMPD). At its value being above 1.0 c.u. one should diagnose pulmonary dysfunction.

EFFECT: higher accuracy of diagnostics.

1 ex

FIELD: medicine, therapy of infectious diseases, hepatology.

SUBSTANCE: it is necessary to carry out clinical survey, moreover, in patients with mechanical jaundice one should determine the percentage of sphingomyelin fraction in phospholipid spectrum of blood serum and at its values ranged 7-15% it is possible to diagnose mechanical jaundice at the background of oncological diseases, and 20-28% - mechanical jaundice at the background of choledocholithiasis.

EFFECT: higher accuracy of differential diagnostics.

3 ex

FIELD: medicine, pulmonology.

SUBSTANCE: in case of doubtful signs of out-patient pneumonia according to the data of roentgenological tests it is necessary to carry out ventilation-perfusion pulmonoscintigraphy with subsequent detection of ventilation-perfusion correlation, alveolar permeability and, also, additional detection of ventilation-perfusion ratio. At simultaneous availability of ventilation-perfusion correlation, the value of ventilation-perfusion ratio being above 1.15 and, also, accelerated, but not less than 30% alveolar permeability of radiopharmpreparation 30 min after its inhalation in the affected lung one should diagnose out-patient pneumonia.

EFFECT: higher accuracy of diagnostics.

2 dwg, 1 ex

FIELD: veterinary science.

SUBSTANCE: the method deals with intravenous injection of mycobacterial suspension at the dosage of 0.1 U for dwarfed rabbits of about 1-1.3 kg body weight. The method is the most informative at detecting the agent's type according to the results of pathologoanatomical trials in dead animals, moreover, the innovation enables to achieve shortened terms for establishing the diagnosis on tuberculosis.

EFFECT: higher efficiency.

2 ex, 3 tbl

FIELD: medicine, gastroenterology.

SUBSTANCE: it is necessary to carry out complex evaluation of clinical values, analyses on dysbacteriosis and coprograms followed by calculating the levels of anxiety, depression, asthenia, somatoautonomic disorders by the following formulas: LAPD=1.13+0.68X1-0.13X2+0.53X3-0.59X4+0.52X5+0.16X6, where LAPD - the level of anxiety-phobic disorders, X1 - the presence and degree of pains at defecation, and at no pains X1=1, at moderate pains X1=2, at pronounced pains X1=3, at pains stopped due to the intake of medicinal preparations X1=4; X2 - the level of Escherichia coli at altered properties in feces expressed in lgCFU/g; X3 - the quantity of muscular fibers altered in feces and at their absence X3=0, at value in analysis being ±X3=0.5, at high values X3=the quantity of crosses; X4 - the presence of hypertonicity of large intestine and at no hypertonicity X4=1, at hypertonicity in area of sigmoid flexure X4=2, at hypertonicity I area of cecum X4=3, at hypertonicity at the whole length of large intestine X4=4; X5 - the presence and the intensity of stomachaches and at their absence X5=1, at weak stomachaches X5=2, at moderate ones X5=3, at intensive stomachaches X5=4; X6 - the level of gold-colored staphylococci in feces expressed in lgCFU/g and at LAPD>3.5 one should diagnose high degree of anxiety-phobic disorders, at 3.5≥LAPD≥2.0 - average degree, at LAPD<2 - low degree; LDD=2.98-0.79X1+0.60X2-0.12X3-0.57X4, where LDD - the level of depressive disorders, X1 - abdominal murmur at stomach palpation and at its absence X1=1, at abdominal murmur in area of sigmoid flexure X1=2, that of in area of cecum X1=3, and at abdominal murmur at the whole its length X1=4; X2 - meteorism and at its absence X2=1, at meteorism at affected nutrition X2=2, at constant meteorism of moderate degree X2=3, at constant pronounced meteorism X2=4, X3 - the level of Escherichia coli at altered properties in feces expressed in lgCFU/g, X4 - the quantity of muscular fibers altered in feces and at its absence X4=0, at values in analysis being ±X4=0.5, at high values X4=the quantity of crosses and at LDD>3.5 one should diagnose high degree of depressive disorders; at3.5≥LDD≥2.0 - average degree, at LDD<2 - low degree; LAD=2.78+0.9X1-0.1X2, where LAD - the level of asthenic disorders, X1 - the quantity of muscular fibers altered in feces and at their absence X1=0, at the value in analysis being ±X1=0.5, at high values X1=the quantity of crosses, X2 - the level of Escherichia coli at altered properties in feces expressed in lgCFU/g and at LAD>3.5 one should diagnose high degree of asthenic disorders, at 3.5≥LAD≥2.0 - average degree, at LAD<2 - low degree; LSAD=4.29-0.18X1-0.18X2+0.32X3-0.15X4-0.84X5-0.95X6+0.93X7-0.55X8+0.83X9+0.13X10, where LSAD - the level of somato-autonomic disorders, X1 - the level of lactobacteria in feces expressed in lgCFU/g, X2 - the level of hemolytic microorganisms in feces expressed in lgCFU/g, X3 - the level of gold-colored staphylococci in feces expressed in lgCFU/g, X4 - the level of epidermal saprophyte staphylococci in feces expressed in lgCFU/g, X5 - the presence of soaps in feces and at their absence X5=0,, at the value in analysis being ±X5=0.5, at high values X5=the quantity of crosses, X6 - the presence of extracellular starch in feces and at its absence X6=0, at the value in analysis being ±X6=0.5, at high values X6=the quantity of crosses, X7 - the presence and the intensity of stomachaches and at their absence X7=1, at weak stomachaches X7=2, at moderate stomachaches X7=3, at intensive ones X7=4, X8 - abdominal murmur at stomach palpation and at its absence X8=1, at abdominal murmur in area of sigmoid flexure X8=2, at murmur in area of cecum X8=3, at murmur at the whole its length X8=4, X9 - the presence of leukocytes in feces and at their absence X9=0, at their presence X9=1, X10 - abdominal murmur and at its absence X10=1, at abdominal murmur rarely than once weekly X10=2, at abdominal murmur being 1-3 times weekly X10=3, and at abdominal murmur above 3 times weekly X10=4 and at LSAD>3.5 one should diagnose high degree of somato-autonomic disorders, at 3.5≥LSAD≥2.0 one should diagnose average degree, at LSAD<2 - low degree. The innovation enables to decrease labor intensity, increase objectivity of evaluation and simplify it for a patient and medicinal staff.

EFFECT: higher accuracy and efficiency of diagnostics.

6 ex

FIELD: medicine, histology, pathohistology, oncology.

SUBSTANCE: it is necessary to conduct computed morphometry of nucleoli stained with 50%-silver nitrate solution The values of nucleoli' areas being within 25-75%% quartiles should be analyzed. Metric parameters of nucleoli areas should be transformed into conditional units of areas - average values of the interval of area referred to the average value of the least interval for normal epithelium which was conditionally considered to correspond to diploid set of DNA nucleus. Based upon frequency characteristics of each interval transformed into conditional units of area it is necessary to obtain histograms to observe qualitative peculiarity in distributing the areas of nucleoli for each clinico-morphological form. The innovation enables to elaborate morphometric classification of the nucleoli associated with nuclear ploidy.

EFFECT: higher efficiency of classification.

2 ex, 2 tbl

FIELD: medicine, clinical toxicology.

SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.

EFFECT: higher accuracy of prediction.

2 ex, 3 tbl