Method for specific prophylaxis of infectious respiratory diseases in calves at group keeping
FIELD: veterinary science.
SUBSTANCE: the present innovation deals with a 2-fold injection of either inactivated or alive vaccine during forming a group. Moreover, the vaccine should be injected on the 1st and the 4th d of animals keeping in a group. Vaccine dosages should be distributed at the ratio of 1:3. The innovation enables to decrease sickness rate and increase the safety of calves in case of different immune state out of the farms of different epizootic situation on viral respiratory infections.
EFFECT: higher efficiency of prophylaxis.
1 ex, 2 tbl
The invention relates to the field of veterinary medicine, in particular to methods of specific prophylaxis of infectious diseases of animals.
In the prevention of infectious animal diseases in agricultural enterprises with a collective population of great importance is the formation of group immunity. Group immunity - immunity, due to the presence of a certain level of specific antibodies or cells of memory to a specific pathogen, group of animals, which are in contact with each other. This indicator is expressed as a percentage; in the presence of 80% or more immune animals, usually an infectious disease in a group does not cover (Voronin Y.S. Immunology / Voronin, E. S., Petrov, A. M., Gray M.M., Gavrilov D.A., Ed. Essaloniki. - M.: Kolos-Press, 2002, - 408; Efanova LI the body's defense mechanisms. Immunodiagnostics and immunoprophylaxis of infectious diseases of animals. Efanova LI, Saiduddin ET - Voronezh: the VSAU, 2004. - S).
The known method of prevention of infectious respiratory disease of calves during the first year of life, including their immunization by a double injection of mono - or associated inactivated or live vaccine with an interval of 10-28 days in doses according to the instruction (Shevyrev NS Introduction to veterinary immunology. The tutorial. - Kursk: the Publishing house XHA, 199, - 249 S.; Veterinary drugs in Russia: a Reference book in 2 volumes. - M.: Selhozizdat. - 2004. - S.219-220).
This provides them with protection from infection after 10-14 days (when using live vaccines), or 21 days (when using inactivated) after repeated administration of the vaccine (Efanova LI the body's defense mechanisms. Immunodiagnostics and immunoprophylaxis of infectious diseases of animals (Efanova LI, Saiduddin ET - Voronezh: the VSAU, 2004, - S. 391).
Prevention of respiratory disease have to spend in animals with different immune status, with different levels of humoral antibodies in their serum, as they come from farms with different epizootic situation on these diseases. Therefore, the introduction of vaccines, especially live, in the same doses, according to the instructions of animals with different levels of specific antibodies does not contribute to the formation of a busy group of humoral immunity. At the same time in the group are animals with different levels of antibodies: high (recovered and vaccinated), low (fading: coastally long vaccinated and has long been ill), no antibody (animals that are not met with the antigen). In animals with high levels of antibodies vaccination does not cause stress immunity, because the antigen is neutralized available antibodies. In the animal without antibodies in the serum insignificant activation of the immune process, because the second administration of the vaccine is in production phase. Animals with low antibody titer, but already having memory cells, capable of generating intense and long-lasting immunity after the first injection of the vaccine, and re-introduction is useless, because the antigen is completely neutralized the produced antibodies. Re-introduction of antigen 10-14 days occurs during the intensive production of antibodies from the first and causes the binding to the antigen IgG, its excretion from the body without causing sufficient repeated exposure to immunocompetent b cells and more intense production of antibodies, as in the productive stage of the accumulation and activation of effector cells and increased antiteloobrazovania: first, IgM and then IgG - up to 10-15 days, and IgA - 15-20 day (Voronin NS Immunology / Voronin NS, Petrov A. M., Gray M.M., Gavrilov D.A. - M.: Kolos-Press, 2002. - P.113-116).
Therefore, the re-introduction of the vaccine in the productive phase of the immune reactions in the same doses in the formation of groups of animals it is expressed group immunity. The part of the population the level of protective antibody.
The objective of the invention is to reduce the incidence and improve the safety of calves in the formation of groups of animals with different Munim status of farms with different epidemic situation on viral respiratory diseases.
The technical result - the reduction of the period of formation of group immunity.
The technical result is achieved in that in the method for the specific prevention of infectious respiratory disease of calves in group content, providing twice the introduction of inactivated or live vaccine, the vaccine is administered in the first and fourth days of the animals in the group, with doses distributed respectively in the ratio of 1:3.
The essence of the proposed method is that the formation of groups of animals under the threat of introduction of pathogens of respiratory disease on the farm, farm, herd calves subjected to vaccination live or inactivated vaccines with antigens of pathogens circulating in the economy of the host animal, or farm suppliers. The vaccine is applied in increasing doses. The total dose for a double injection, provided in the instruction, divided as follows: first time being 1/4 of the total dose in 1 day, keeping of animals in the group, the second - 3/4 part of the total dose on day 4.
Fractional introduction of antigen aligns the formation of immune responses in animals with different immune status. In animals with high levels of antibodies at the first introduction of a half dose of antigen is neutralized by antibodies, and increased re d is for 3 days only partially neutralized by antibodies and partially activates the memory cells to stimulate the immune process in a more expedient manner. In animals with residual levels of antibodies in the blood of the first dose of antigen activates memory cells and activates the immune changes in the body, and the re-introduction of increased dose of vaccine coincides with the inductive phase and further activates immune cells. In animals without antibodies in the blood - the first dose activates the launch of the immune response, and increased second as well as the previous version arrives in the inductive phase of the immune response. In the blood sera at the moment has almost no post-vaccination antibodies formed on the first introduction of the vaccine, which could partially antigen to neutralize. Thus, immunity is formed and activated in calves with different levels of antibodies within 7-21 days after the second injection. Characterized by marked cellular response to the accumulation of a sufficient number of specific agglutinins the whole group of animals. For 10 days reduced the time of formation of immunity that under the threat of infection in animals in the formation of groups is of great importance. By the end of the 30-day quarantine group of animals that will be housed in the same premises, acquires a distinct group immunity.
Example. The complex is e with a collective population was conducted twice immunization of calves against respiratory disease of young cattle vaccine "Trivac" polyvalent associated against parainfluenza-3 (PG-3), infectious bovine rhinotracheitis (RTI), viral diarrhea is a disease of the mucous membranes (BD-BS). (Temporary instruction on the use of vaccines "Trivac" polyvalent dry associate - Biological drugs in Russia: vaccines, serum-based assays: a Handbook, Iaanano. - Kazan: Rutin, 2005 - P.17). The vaccine was administered subcutaneously, in the middle third of the neck. In the experiment were 4 groups of animals: group 1 (n=30) - vaccinated with an interval of 14 days (on the 1st and 15th days of quarantine) at doses of respectively 2 and 2 ml; group 2 (n=30) - vaccinated with an interval of 3 days (at 1 and 4 days of quarantine), at doses of 2 and 2 ml; group 3 (n=30) - vaccinated with an interval of 14 days (on the 1st and 15th days of quarantine), in doses of 1 and 3 ml; group 4 (n=30) - vaccinated with an interval of 3 days (at 1 and 4 days of quarantine), in doses of respectively 1 and 3 ml.
The final results evaluated comprehensively based on the results of disease, severity of disease, the level of specific antibodies in the serum to antigens contained in the vaccine. Serological analysis of serum samples was performed in 10 animals from each group.
Table 1 shows the performance of titers of antibodies to pathogens parainfluenza-3, infectious bovine rhinotracheitis viral diarrhea in cattle and number of animals with positive and negative titers to these pathogens prior to vaccination and p is after vaccination.
|The name of the antigens, the number of animals||1-day quarantine||On the 15th day quarantine||30-day quarantine|
|Groups of animals||Groups of animals|
|Number of animals* (n=10)||4/6||5/5||10/0||7/3||10/0||10/0||10/0||7/3||10/0|
|A positive titer||1:16|
|To the number of animals* (n=10)||4/6||6/4||10/0||8/2||10/0||6/4||10/0||10/0||10/0|
|A positive titer||1:16|
|Number of animals* (n=10)||8/2||6/4||8/2||6/4||10/0||6/4||10/0||6/4||10/0|
|Note: * numerator - number of animals with protective titers and above;|
denominator is number of animals that do not have antibodies and having the antibody titers less protective.
From table 1 it is seen that the 4-th group on the 15th day quarantine in 100 surveyed animals in all of the studied antigens were obtained protective antibody titers (PG-3 1:1160, IRT - 1:58, VD-BC - 1:99), while in the 2nd group, this result was re-examined on a 30 day quarantine, and in the 1st and 3rd groups he was on a 30-day quarantine, respectively, PG-3 - 100 and 70%IRT 60 and 100%, VD-BS - 60 and 60%.
Table 2 shows the performance of the vaccine under different vaccination schedules.p>
|1-I (n=30)||2-I (n=30)||3-I (n=30)||4-I (n=30)|
|Culling (removal from rearing)||10||3,3||10||3,3|
|Duration of illness (days)||6||4||5||4|
Out of the 30 calves in group 1 ill 20 goals and was removed from the rearing - 3 heads, 2-y, respectively, 10 and 1 goal., in the 3rd - 18 and goal 3., 4 th - 6 and 1 goal.
Table 2 shows that among calves in quarantine incidence in the groups ranged from 30 to 80%off rearing and 3.3 - 10%, disease duration 4-6 days.
The incidence and severity of disease was lowest in the 4-th group, respectively, 20% and 4 days. And disease in calves proceeded in a more mild form.
The proposed method is tested on farms Voronezh, Lipetsk, Belgorod regions with a positive result. Improved epizootic situation in animal husbandry with a collective population, permanently affected respirato the ailments of young animals.
The way of specific prophylaxis of infectious respiratory disease of calves in group content, providing twice the introduction of inactivated or live vaccine when forming groups, wherein the vaccine is administered in the first and fourth days of the animals in the group, with doses of vaccine distributed respectively in the ratio of 1:3.
FIELD: biotechnology, in particular GHRH-fixing protein.
SUBSTANCE: disclosed is new protein having molecular mass of about 90.9 kDa which includes fragments of identified peptide sequences 1, 2 and 3. Method for production of said protein includes extraction of Pilocarpus heterophyllus plant cells with water, vacuum filtering, deposition of extracted proteins, centrifugation, and gel filtering of obtained filtrate. Also produced is monoclonal antibody which specifically fixes isolated protein.
EFFECT: agent for producing of effective drugs and pharmaceutical composition against GHRH action and treatment of proliferative diseases.
12 cl, 5 dwg, 2 ex
SUBSTANCE: invention characterizes compositions, their employment, and embodiments of a method of treatment of B-cellular lymphomas, leucosis, and other malignant tumors CD40+. Principal active therapeutical agent is anti-CD40L antibody or another CD40L antagonist inhibiting CD40-CD40L intermediate. In combination or composition with indicated CD40L antagonist any one or several of the following components can be additionally used: anti-CD20 antibody, a chemotherapeutical agent or a combination thereof, and radiotherapy.
EFFECT: enhanced mechanisms of apoptosis of tumor CD40+ cells due to sensitization of these earlier destruction-resistant cells.
88 cl, 4 dwg, 6 tbl, 8 ex
FIELD: gene engineering, virology, veterinary.
SUBSTANCE: cattle vaccine contains plasmid including F gene, G gene or F and G genes of bovine respiratory syncitial virus. Vaccine of present invention in useful in cattle breeding for bovine immunization against respiratory syncitial virus.
EFFECT: new vaccine against bovine respiratory pathologies.
7 cl, 11 dwg, 16 ex
SUBSTANCE: porcine vaccine includes plasmid including gene of PRRS virus. PRRS gene is selected from group of E, ORF3, and M genes. Vaccine excites immune response against PRRS virus and is useful in pig breeding.
EFFECT: new vaccine against porcine respiratory and reproductive pathologies.
10 cl, 25 dwg, 26 ex
FIELD: pediatrics, otolaryngology, and infection diseases.
SUBSTANCE: treatment comprises determining level of secretory immunoglobulins sIgG and sIgA in saliva before administration of immunomodulator "IRS 19" and 7 days after administration. In case of increase of sIgA against reduction of sIgG, antibacterial therapy is not prescribed. If increase of sIgG is observed against reduction of sIgA or no changes in the latter, wide action spectrum antibiotics are prescribed in age-specific doses and in generally accepted administration schemes.
EFFECT: achieved long-term stable remission with minimum side effects.
2 tbl, 2 ex
SUBSTANCE: immunodiffusion reaction in gel is carried out with living cultures of analyzed strains B.mallei and B.pseudomallei and obtained rabbit antiserum is used as immune serum against exocellular antigens (ECA) of strain Burkholderia thialandensis 264. Analyzed strains B.mallei and B.pseudomallei are seeded on plate with solid mutrient medium (F-agar "Difco"), incubated for 18 h at 37°C. In agar wells are punched nearly of grown plaques and abovementioned rabbit antiserum is introduced. Plates are exposed for 18 h at 24°C and reaction results are analyzed. Presence of precipitation lines between plaques of seeded cultures and antiserum indicates seeding of bacterium B.pseudomallei. Absence of such lines indicates seeding of bacterium B.mallei. Antiserum is obtained by rabbit immunization with extracellular antigens of strain B.thialandensis 264 which represent filtrates of swab of bacterial mass grown on solid nutrient medium coated with cellophane. Filtrates are sterilized in two steps on membrane filters (pore size 0.45 mum and 0.22 mum). Extracellular filtrate components are precipitated with acetone, dried and used for rabbit immunization. Serum activity is evaluated by immunodiffusion reaction in gel. Blood is sampled at serum titer of 1:32 or more.
EFFECT: simplified method of improved accuracy.
2 cl, 4 ex
FIELD: immunology, medicine.
SUBSTANCE: claimed recombinant antibody (Ab) has at least constant regions in heavy and light chains representing human Ab regions. Said At inhibits bonding of integrine recognizing RGD and SVVYGLR sequences to integrine or fragment thereof. Also disclosed are nucleotide sequences (NS) encoding heavy and light chains of recombinant Ab as well as expression vectors containing respective NS. Described are host cell for Ab production, transformed with two vectors for expression of Ab heavy and light chains and method for abovementioned host cell application to produce recombinant Ab. Ab of present invention is useful in diagnosis and treatment of autoimmune diseases, rheumatism and rheumatoid arthritis.
EFFECT: therapeutic methods of increased efficiency.
45 cl, 14 tbl, 28 ex
SUBSTANCE: method for isolating antibodies involves a procedure of adsorption chromatography using water-insoluble uncharged adsorbent (variant 1), or a procedure for desalting antibodies from egg yolk aqueous extract resulting to specific precipitation of IgY antibodies (variant 2), or using a successive procedure of adsorption chromatography (by variant 1) and desalting procedure (variant 2) in combination (variant 3). Invention provides carrying out the selective purification of antibodies IgY (▵Fc) and IgY from lipids under conditions of their increased content in egg yolk of Anseres order birds. Invention can be used for quantitative and qualitative analysis or preparing pharmaceutical compositions directed to etiological factor representing the interest.
EFFECT: improved preparing method.
23 cl, 4 dwg, 2 tbl, 6 ex
FIELD: veterinary microbiology.
SUBSTANCE: according to the first variant, the vaccine suggested includes VGNKI-3/7 strain of hen's pox virus as a virus-containing material; as a stabilizer one should apply the mixture of peptone and lactose in 0.1-0.2M-phosphate buffer at pH being 7.0-7.4 at the following ratio of components, weight%: virus-containing material out of VGNKI-3/7 strain of hen's pox virus at infection activity being about 5.0-7.0 ID50/cu. cm 65.0-70.0; peptone 4.0-10.0; lactose 5.0-10.0; 0.1-0.2M-phosphate buffer at pH 7.0-7.4 - the rest. According to the second variant, the vaccine suggested contains VGNKI-3/7 strain of hen's pox virus as virus-containing material, and as a stabilizer - glycerol at the following ratio of components, weight%: glycerol 5-10; virus-containing material out of VGNKI-3/7 of hen's pox virus at infection activity of 6.0-7.5 lg ID50/cu. cm - the rest. The vaccine creates prolonged tense immunity.
EFFECT: higher efficiency.
3 cl, 9 ex, 1 tbl
SUBSTANCE: method involves applying alkaline extraction from raw material. Daphnias are used as initial raw material, preliminary degreased with ethylic ether, dried up and crushed up to powder condition. The fat-free powder is extracted with 0.1 mole/l sodium hydroxide solution during 3 days at temperature of 4-8°C. Supernatant liquid is poured out after having finished extracting and protein is sedimented from alkaline extract with sodium benzoate as follows: 20 g of sodium benzoate is available in 1 l of alkaline extract, alkaline extract рН is reduced to 3.7. Sedimented benzoic acid is precipitated with the adsorbed allergen by centrifuging within 5 min at 2000 rpm. Allergen desorbed with benzoic acids by adding to acetone deposit. The mixture is stirred to completely dissolve benzoic acid and then it is places for 12-18 h into refrigerator at -20°С temperature. The solution is filtered, waiting 18-20 hours for full evaporation of acetone traces. Allergen powder is extracted during 3 days. Allergenic extract is centrifuged at 5000-6000 rpm within 30-40 minutes with sterilizing filtration following until ready form of diagnostic allergen being obtained. The produced preparation has been studied according to methodical instructions to primary experimentally-clinical approbation of new forms of allergens.
EFFECT: reduced risk of adverse side effects; high specific activity; no animal sensitization when given in the diagnostic doses.
SUBSTANCE: method involves evaluating male birds as to live weight characteristics at 5-week age, chest musculature, and hardness of skeletal frame; additionally, taking into account the number of dents on foliate cockscomb at 17-18-week age; discarding male birds with 3, 9 and more dents on foliate cockscomb.
EFFECT: increased impregnation capacity of eggs.
2 tbl, 1 ex
FIELD: veterinary medicine.
SUBSTANCE: the present innovation deals with sampling a cow's vaginal smear followed by its staining and microscopic testing the cells. During microscopic testing one should count the cells of vaginal epithelium (surface, parabasal and basal) . In case of prevailing quantity of surface cells (49-60%) one should diagnose normal flow of puerperal period, in case of prevailing parabasal epithelial cells one should diagnose complicated puerperal period, moreover, if the quantity of surface and basal cells is similar or he difference is insignificant (15-20% against 15%, correspondingly) it is possible to diagnose uterine subinvolution, and at considerable difference in the quantity of surface and basal cells (0.5-11% against 20-22%) one should diagnose acute puerperal endometritis. The innovation enables to analyze and detect puerperal pathology in earlier terms.
EFFECT: higher accuracy of diagnostics.
1 dwg, 2 tbl
FIELD: poultry farming.
SUBSTANCE: method involves introducing iodous starch into portion of 28-day aged broiler chickens by single subcutaneous injection, with iodine dose making 2-4 mg/head.
EFFECT: increased live weight gain of broiler chickens per unit of consumed feed and improved quality of product.
2 tbl, 2 ex
FIELD: poultry farming.
SUBSTANCE: method involves introducing iodous starch into ration of laying hens at 7-month age by subcutaneous injection, with iodous starch being introduced in a dose of 1.5-3.0 mg/head.
EFFECT: increased egg production capacity per unit of consumed feed.
2 tbl, 2 ex
SUBSTANCE: method involves raising animals in brooder of isolated sections, with transition through said sections being provided according to processing order; forming mini-herds from pigs of adjacent brooders by providing for visual communication and immediate contacting therebetween, with beginning of said contacting being regulated by opening of passageways in partition walls of adjacent brooders.
EFFECT: reduced stress during pig grouping, increased animal productivity, and reduced pig raising time.
2 dwg, 1 ex
FIELD: animal science.
SUBSTANCE: for the purpose to obtain farm animals of preset sex it is necessary to apply male spermatozoa to detect sex sign in certain multitude of spermatozoa and sort spermatozoa according to the detected sex sign and collect them into the collector supplied with a shock absorbing element. Then one should obtain a sorted combination of spermatozoa to introduce it for female of the given mammalian type to fertilize, at least, one female ovicell at resultativeness level being, at least, 50% against that of pregnancy achieved at applying conventional combination of spermatozoa for artificial insemination. For separating spermatozoa one should create the source of cells that supplies spermatozoa for sorting procedure. Chemically one should coordinate capsular liquid for creating the medium out of capsular liquid for spermatozoa being coordinated with liquid medium of spermatozoa both before and after sorting technique. Then it is important to identify one of spermatozoa's properties. Spermatozoa should be differentiated in accordance to their sex and separated at the rate of, at least, 1200 operations/sec. Then spermatozoa with desired sex sign should be collected into the collector supplied with shock-absorbing element. To obtain multiple embryos it is necessary to induce polyovulation in a female of the given mammalian type to obtain, at least, two ovicells. Moreover, a polyovulation-favoring pharmaceutical preparation should be introduced at 12-h-long interval at any days between the 2nd and the 18th d of estrus cycle. It is necessary to detect sex in certain multitude of spermatozoa in a male of the given mammalian type to separate these spermatozoa according to sex sign and introduce separated spermatozoa into female uterus of the given mammalian type after estrus onset to fertilize multitude of ovicells in the uterus. The innovation enables to efficiently separate male spermatozoa in farm animals and obtain from them the offspring of desired sex and, also, obtain multiple embryos of the preset sex.
EFFECT: higher efficiency of artificial insemination.
70 cl, 4 dwg, 4 ex
SUBSTANCE: naturally occurring minerals suitable for use in enterosorption process, especially in sorption of nickel and lead, are proposed. Zeolite originated from Kamyshlovsk deposit situated in Sverdlovsk region is added to main dairy cow diet in amount 0.15-0.20 g per 1 kg animal weight a day over a 25-30 day period.
EFFECT: achieved environmentally safe produce.
FIELD: animal science.
SUBSTANCE: it is necessary to introduce biologically active additives "Gorasel" or "Tykvorsel" being selenopyrane solutions in the mixture of vegetable oils. These biologically active additives should be injected subcutaneously per 10 ml/cow 4 times: the first time - immediately afterlaunching, and then 30, 20 and 10 d before calving. The innovation suggested enables to increase milk yield and milk quality.
EFFECT: higher efficiency.
FIELD: animal science.
SUBSTANCE: it is necessary to introduce perorally an alcohol-honey extract of walnuts of milk-wax maturity at adding glycine and flower pollen. The preparation should be introduced 5 d before and after the impact of stress factors 30 min before feeding per 10-15 ml/animal daily. As a result of applying the suggested preparation the body weight losses in animals are decreased considerably, the body weight gain is increased along with the quantity and quality of animal production.
EFFECT: higher efficiency of prophylaxis and correction.
1 ex, 2 tbl
FIELD: veterinary science.
SUBSTANCE: the innovation deals with supplementing the diet with iodinated common salt and a medicinal preparation perorally as metronidasol and a preparation of sulfanyl amide group. Additionally, one should introduce a diuretic, and as medicinal preparation one should additionally use penicillin sodium salt intramuscularly and 0.5%-metronidasol solution intravenously. Moreover, for pregnant cows of dairy herd the preparations should be introduced once annually for the whole productive period in the following sequence: a 6-d-long course up to calving dealing with introducing metronidasol at the dosage of 1.5 g/animal and norsulfasol or sulfadimezine at the dosage of 1 g/animal. At calving day it is necessary to inject intravenously 0.5%-metronidasol solution at the dosage of 200 ml/animal thrice in 8 h. Since the 2nd to the 5th d inclusively it is important to introduce metronidasol perorally at the dosage of 2.5 g/animal and norsulfasol or sulfadimezine at the dosage of 1 g/animal. Since the 6th to the 15th d inclusively one should inject intramuscularly penicillin sodium salt at the dosage of 2 mln U/animal - twice daily. On the 16th d one should inject intravenously 0.5%-metronidasol solution at the dosage per 200 ml/animal thrice in 8 h. With prophylactic purpose for stud bulls it is necessary to introduce preparations twice annually at 6-mo-long intervals according to the following scheme: during the 1st and the 2nd d - intravenously 0.5%-metronidasol solution at the dosage of 300 ml/animal thrice at 8-10-h-long interval. Since the 3d to the 5th d inclusively - perorally metronidasol at the dosage of 2.5 g/animal and norsulfasol or streptocid at the dosage of 2 g/animal. Since the 6th to the 15th d - intramuscularly penicillin sodium salt at the dosage per 3 mln U/animal twice daily. On the 16th d - intravenously 0.5%-metronidasol solution at the dosage of 300 ml/animal thrice in 8 h. With prophylactic purpose for cattle youngsters the preparations should be introduced starting since 1-mo age according to the following scheme: during the 1st d - intravenously 0.5%-metronidasol solution at the dosage of 10 ml/animal thrice at 8-10-h-long interval. During ten days - perorally metronidasol at the dosage of 0.125 g/animal and norsulfasol at the dosage of 0.5 g/animal once daily. On the 13th and the 14th d - intravenously 0.5%-metronidasol solution at the dosage of 10 ml/animal thrice at 8-10-h-long interval, for 4-mo-aged calves during the first 2 days - intravenously 0.5%-metronidasol solution at the dosage of 20 ml/animal thrice at 8-10-h-long interval. During 18 days - perorally metronidasol at the dosage of 0.5g/animal and norsulfasol at the dosage of 0.5g/animal once daily, for 6-mo-aged calves during 10 days running - intramuscularly penicillin sodium salt at the dosage of 500 thousand U/animal twice daily. For 12-mo-aged calves during the first 2 days - intravenously 0.5%-metronidasol solution at the dosage of 100 ml/animal thrice at 8-10-h-long interval, since the 3d to the 5th d inclusively - perorally metronidasol at the dosage of 1 g/animal and norsulfasol or streptocid at the dosage of 1 g/animal once daily, since the 6th to the 12th d inclusively - intramuscularly penicillin sodium salt at the dosage of 1 mln U/animal twice daily. For 17-mo-aged calves during the first 2 days - intravenously 0.5%-metronidasol solution at the dosage of 150 ml/animal thrice at 8-10-h-long interval; since the 3d to the 5th d - perorally metronidasol at the dosage of 1.5 g/animal and norsulfasol at the dosage of 1 g/animal; for 18-mo-aged calves before pairing for 10 d - intramuscularly penicillin sodium salt at the dosage of 1 mln U/animal twice daily. The innovation is of high efficiency and enables to shorten the terms of therapy along with widened groups of animals under treatment and increased action of medicinal preparations.
EFFECT: higher efficiency.
FIELD: animal science.
SUBSTANCE: the present innovation deals with dynamic loading onto cardio-vascular system in animals. Selection should be carried out by the following parameters: , ΔT3 and Δn, where ΔT1 - the time for pulse increase at running, ΔT2 - the time for pulse stabilization after running, ΔT3 - the time for pulse increase after running, Δn - the increase of pulse frequency after running. One should select animals into milking herd at the following values; ΔT3 ≤ 10 sec, Δn ≤ 10 beats/min. The method enables to present perspective evaluation of lactation capacity in animals.
EFFECT: higher efficiency of selection.
1 dwg, 1 ex, 1 tbl