FIELD: pharmaceutical industry, in particular biocide agent.
SUBSTANCE: claimed gel includes hydroxyethyl cellulose, polyguanidine compound (e.g. polyhexamethylene guanidine succinate or poly-(4,9-dioxadodecan guanidine) succinate), glycerol, trilon B, polyethylene glycol, citric acid, perfume composition, and water. Additionally it contains neonol, calcium pantothenate and chamomile extract in specific component ratio.
EFFECT: gel with increased biocidal action.
3 tbl, 3 ex
The invention relates to medicine and can be used in surgery, obstetrics, emergency stations, hospitals and other medical institutions. The gel can also be used in the food industry, public utilities, public power and in everyday life.
This vast and varied scope of the inventive gel due to its properties and effect: it has a disinfectant, antibacterial, antiseptic, antibacterial properties.
Known gel for antiseptic treatment of skin, containing in its composition as an antiseptic Miramistin (U.S. Pat. Of the Russian Federation No. 2185157, CL AC 31/14, 2000).
The disadvantages of this gel is that applied Miramistin is a derived group of biguanidine and not have a prolonged effect. The scope of Miramistin limited. At the same time, the antibacterial activity of Miramistin is extremely low.
The closest solution to the present invention is a hygienic gel of the following composition, wt.%:
|- methylcellulose or hydroxyethylcellulose||0,3-3,0|
|- glycerin||0.5 to 10.0|
|- citric acid||0,5-3,0|
|- Trilon B - sodium salt|
|connection of polyguanidine or|
|synergistic mixture of Quaternary|
In the gel hygienic as connection polyguanidine use phosphate poly-(4,9-dioxadodecane), or citrate/formate, guanidine, or gluconate/benzoate, guanidine, or chloride, guanidine (U.S. Pat. Of the Russian Federation No. 2225202 B. I. No. 7, 2004).
However, the known gel has a number of disadvantages: the use of cremophor a negative effect on the technological properties of the gel, namely deteriorating penetration of the gel into the skin. In addition, the presence of cremophor in the composition of the gel leads to a deterioration of the homogeneity of the composition during storage. It is also noted that the presence of dimethylbenzylamine sulfate impedes the penetration of the gel through to the, because of what decreases the bactericidal effect.
The objective of the invention is to increase the biocidal effect and reduce side effects from the use of gel.
To solve the problem in the gel biocidal, including hydroxyethyl cellulose, the connection of polyguanidine, glycerin, cremophor, Trilon B, glycol, citric acid, perfume composition and water quality of the connection of polyguanidine use of polyhexamethylene guanidine succinate or succinate poly-(4,9-dioxadodecane), and it additionally contains neonol, calcium Pantothenate and chamomile extract in the following ratio, wt.%:
|or succinate poly-(4,9-dioxadodecane)||0,3-3,0|
|Polyethylene glycol PEG-400||1,0-5,0|
|The polyethylene glycol is PEG-40||1,0-1,5|
The invention is illustrated as follows.
Highly potent compounds of polyguanidine have a broad spectrum of biocidal activity: antibacterial, fungicidal, virucidal. They have low toxicity, does not penetrate intact skin (IV class of danger), saved it for a long time, protecting it from exposure to the environment.
As compounds of polyguanidine use succinate pgmg (disubstituted) or succinate poly-(4,9-dioxadodecane) (disubstituted).
These compounds possess a wide spectrum of biocidal activity.
Succinate pgmg (disubstituted) has the following structural formula:
Succinate poly-(4,9-dioxadodecane) (disubstituted) has the following structural formula:
They can be obtained as follows.
The action of alcohol CON from pgmg chloride or chloride poly-(4,9-dioxadodecane) are removed completely ions Cl-and alcohol solution of the obtained bases polyguanidine are alcoholic solution of succinic acid. The alcohol is distilled off and get anhydrous solid salt succinate pgmg or succinate p is whether-(4,9-dioxadodecane) (disubstituted).
Succinic acid, which is part of succinate pgmg and poly-(4,9-dioxadodecane)is bioactive natural compound that is present in the human body and therefore salt of succinic acid necessary for human body. Biocidal effect of the gel is increased due to the fact that succinate pgmg and succinate poly-(4,9-dioxadodecane) contributes to a more prolonged biocidal effect of the gel after application to the skin and penetration.
Used in the composition of the gel as the gel-forming component hydroxyethylcellulose is a powder of white or grayish color with the main component content not less than 97 wt.% (THE 6-05-1857-87).
Glycerin (TU-6259-25) is a transparent liquid with a basic substance content not less than 99%. Glycerin - enveloping and astringent component. Provides skin soothing and astringent effect, promotes penetration into the skin active agents, activates metabolic processes. The use of glycerol increases the efficacy of antiseptic.
Neonol - non-ionic surfactant is in Russia, comes in the form of 100% viscous water-soluble liquid (TU 6-05-1857-87), detergent for use in shampoos, gels for body and other Structural formula is as follows:
Polyethylene glycol (PEG-400) (TU-6-00205601.083-2000) is a viscous or waxy substance, soluble in organic solvents, sparingly in water, precipitates from aqueous solutions at temperatures close to 100°C. the polyethylene Glycol is used as a thickener and binder. PEG-40 - viscous waxy substance is hydrogenated castor oil.
When applying guanidino antiseptics in gel PEG soften their effect on the skin and enhances the biocidal effect. PEG is able to penetrate into the subcutaneous tissue and transported there drugs, as well as to improve the stability of the composition over time, to improve wetting and regenerative ability of the gel. In addition, it also improves the penetrating ability of the gel, which is beneficial to its medicinal properties.
Calcium Pantothenate calcium salt of D-(+) Pantothenic acid. Pharmacological action replenishes the deficiency of Pantothenic acid, softens the skin, facilitates the absorption of the components of the gel in particular, succinate pgmg and succinate poly-(4,9-dioxadodecane).
Trilon B (GOST-10652) is a sodium salt of ethylenediaminetetraacetic acid, complex III is an organic chelate forming compound, crystallises is e, soluble in water, ethanol and most organic solvents.
Used as a titrant in complexometry, for division and separation of metal ions to dissolve and prevent the formation of various sediments, caused, for example, water hardness, for excretion from the body of toxic metals, stabilization of food, water softener, as components of detergents.
Citric acid (GOST-3652) is a colorless crystals with monoclinic lattice. Solubility in water, % (temperature, °): 59,2 (20), 84 (100); molecular weight: 192,2. Apply the gel as complexing agents and pH regulator. Shows the properties of polybasic carboxylic acids, forms three series of salts and esters (citrates) carboxyl groups.
Citric acid and its salts are used in the food industry as flavouring substances as components of the buffer solutions. To prevent fats from spoiling, in the pharmaceutical industry as components of many drugs, sodium citrate is an anticoagulant.
Chamomile extract is used in the gel as a component with anti-allergic and anti-inflammatory action.
Fragrance composition is an organic liquid with a characteristic party Arnim smell. Use different songs with fruity floral, fruit and herbal, spicy, pine aromas. The most preferred odorants JSC "Balm" (Russia, Nizhny Novgorod).
As the basis in the cream composition using distilled water (GOST 609-84).
The quantitative content of the components of the gel, recommended by us, is optimal and selected on the basis of numerous experiments.
Table 1 presents data confirming the optimality of the claimed ratios of the components, as well as the data that go beyond the stated ratios.
The process of making gel disinfectant includes preparation of raw materials and equipment, weighing components, the dispersion of hydroxyethyl cellulose in glycerol in the presence of neonols for 1.5-2 hours at a temperature of 30-40°with periodic mixing.
Separately preparing an aqueous solution getcomponent by dissolving in water.
Aqueous solutions of PEG-400 and PEG-40 cook with constant stirring. Separately prepared aqueous solution of calcium Pantothenate and an aqueous solution of the trylon.
Almost the preparation of a disinfectant gel as follows: in the reactor pour the prepared solution of hydroxyethyl cellulose in glycerol and surfactant, an aqueous solution of PEG-400 and PEG-40, an aqueous solution of descampe the NTA, aqueous solutions of calcium Pantothenate and trylon B, the perfume composition. After mixing, add citric acid to bring the pH of the prepared mixture to the desired neutral level.
The resulting mass is diluted with water in accordance with the recipe, continuously stirring at a temperature of 25-30°to obtain a homogeneous solution.
Testing of antimicrobial activity designed disinfectant gel used 2 ways. By the first method defined zone of inhibition of microorganisms by diffusion of the analyte in a dense nutrient medium (Fedorov Y.A., Root NR. "Microbiological research methods in dental hygiene products" in the book. "Basics of oral hygiene". L.: "Medicine", 1973, p.3-215).
The Petri dish was filled with 20 ml of a dense nutrient medium. Prepared 24 hours a microbial suspension of test microorganism with a concentration of 5×105CFU/ml and applied to 1 ml of the suspension on the surface of the medium. Was dried for 30 min, and then in a nutrient medium made holes with a diameter of 6 mm, which was made samples developed disinfectant gel. The number of holes should not exceed 6 per Petri dish. For 30 min in Petri dishes kept at room temperature (20°C), then they were placed in the incubator for 24-48 hours in temp is the temperature 37° And after a specified period of time measured the zone of inhibition of microbial growth, including the diameter of the hole. The size of zones of inhibition of growth of test microorganisms was judged on the antimicrobial activity of the test substance, although it should be considered that the diffusion rate of the polymeric disinfectants lower rate of diffusion of low molecular weight disinfectants.
To obtain reliable test results of each analyte performed in 2-3 series of replications.
The second way to test antimicrobial activity of gel consists of determining the minimum time required for complete suppression of the growth and development of test microorganisms.
In the test substance was added to a suspension of the test organisms in the amount of 106CFU/ml in a test tube pour 1 ml of the test substance. The tubes were shaken and intervals 2-5-10 and 30 minutes did the sowing on the solid nutrient medium. Petri dishes were placed in 24 to 48 hours in a thermostat at 37°C. After the expiration of that period were counting the number of grown colonies and this indicator was judged on the bactericidal activity of the test substance.
The results of these studies are presented in table 2.
From the presented data in table 2 it follows that a more sensitive test means b is Li gram-positive organisms (S. aureus). All test tools formed a zone of stunted growth from 22 to 35 mm, which is sufficiently severe or high activity. Draws attention to the high activity of the preparation according to example 3 to gram-negative microorganisms of the species E. coli and Ps. aeruginosa.
When determining the speed of the manifestations of antimicrobial activity test tools the following results were obtained (table 3).
From the presented data in table 3 it follows that the antibacterial effect of the developed formulations is developing very quickly. After 2 min of contact in any case not found living microflora after contamination of the nutrient medium by Escherichia coli.
To verify the validity of disinfectant gel were tested on volunteers in accredited specialized Center. Marked highly effective action of the gel as a disinfectant on the skin.
There was also a certain therapeutic effect of gel in the treatment of purulent wounds and ulcers. Marked healing effect without the formation of superficial scars.
Special experiments showed that succinate pgmg and succinate poly-(4,9-dioxadodecane) unlike pgmg hydrochloride pgmg-phosphate active against spore-forming bacteria. This extends the application of the gel.
In the result of the verification is built Toxicological studies have established the that the gel is completely harmless, irritating and allergenic action.
Example 1. Content, wt.%:
Gel well onto skin, consumed sparingly and quickly absorbed. Promotes the protection and restoration of the damaged structures of the skin. On the skin surface is formed an invisible polymer film that protects the skin and provides its sterility until then, until it is washed off.
The gel can be used for processing of the arms trade workers, food industry workers, and also the hands of the surgeon before the operation. After drying of the gel on the skin surface has reached what is bactericidal effect of gloves.
Example 2. Content, wt.%:
When processing the feet of the obtained gel eliminates the smell of sweat, protects against infection by a fungus that kills pathogens. The gel can be used for processing Shoe after wearing.
Example 3. Content, wt.%:
This composition can be used for healing of wounds, abrasions, sores, burns of various origins, wet eczema, fungal infections of the skin. Especially it is effective when the dressing with the gel on the festering wound. After treatment gel purulent wounds and wounds with uneven edges were observed scarring.
It should be noted that the disinfectant gel in all of these examples, as well as examples of table 1 starts instantly and biocidal effect persists for at least 5 hours.
The safety of the gel and its homogeneous composition determines mainly the active substance - succinate pgmg or succinate poly-(4,9-dioxadodecane). The shelf life of the gel reaches 5 years. During this period, its color does not change, and disinfectant properties are not lost.
|No. Ave||Hydroxyethylcellulose||Succinate pgmg||Succinate poly-(4,9-dioxadodecane-guanidine||PEG-400||PEG-40||Glycerin||Calcium Pantothenate||Neonol||Trilon B||Chamomile extract||Citric acid||Perfume||Water||Note|
|1||1,0||-||2,0||5,0||1,0||5,0||2,0||2,0||1,0||1,0||3,0||0,3||leave||Homogeneous transparent gel with prolonged disinfectant effect.|
|3||0,5||3,0||-||3,0||1,2||1,0||1,0||1,0||2,0||0,5||0,1||0,5||leave||Supports the natural moisture balance in the skin.|
|6||2,2*||1,0||-||2,0||1,2||4,0||2,0||2,0||1,0||0,5||4,0||0,5||leave||Too thick gel.|
|7||2,0||-||0,1*||2,0||1,2||4,0||2,0||2,0||1,0||0,5||4,0||0,5||leave||Not enough disinfectant effect.|
|8||2,0||-||3,2*||2,0||1,2||4,0||2,0||2,0||1,0||0,5||4,0||0,5||leave||Too high costs of disinfectant.|
|10||2,0||-||1,0||5,3*||1,2||4,0||2,0||2,0||1,0||0,5||4,0||0,5||leave||The gelling of the gel.|
|11||2,0||-||1,0||2,0||1,0*||4,0||2,0||2,0||1,0||0,5||4,0||0,5||leave||Deterioration requirements. properties of the gel.|
|12||2,0||-||1,0||2,0||1,7*||4,0||2,0||2,0||1,0||0,5||4,0||0,5||leave||The increase in viscosity of the gel.|
|13||2,0||-||1,0||2,0||1,2||0,8*||2,0||2,0||1,0||0,5||4,0||0,5||leave||Poor absorption into the skin.|
|14||2,0||-||1,0||2,0||1,2||10,3*||2,0||2,0||1,0||0,5||4,0||0,5||leave||The gelling of the gel.|
|15||2,0||-||1,0||2,0||1,2||4,0||0,05*||2,0||1,0||0,5||4,0||0,5||leave||The decrease in biological activity.|
|16||2,0||-||1,0||2,0||1,2||4,0||2,1*||2,0||1,0||0,5||4,0||0,5||leave||Unnecessarily high consumption of the component.|
|17||2,0||-||1,0||2,0||1,2||4,0||2,0||0,5*||1,0||0,5||4,0||0,5||leave||The deterioration of the stability of the gel.|
|18||2,0||-||1,0||2,0||1,2||4,0||2,0||3,1*||1,0||0,5||4,0||0,5||leave||Leads to the formation and loss of antimicrobial activity.|
|19||2,0||-||1,0||2,0||1,2||4,0||2,0||2,0||0,05*||0,5||4,0||0,5||leave||The deterioration of the stability of the gel.|
|20||2,0||-||1,0||2,0||1,2||4,0||2,0||2,0||2,1*||0,5||4,0||0,5||leave||The decrease in antimicrobial activity.|
|21||2,0||-||1,0||2,0||1,2||4,0||2,0||2,0||1,0||0,25*||4,0||0,5||leave||The reduction of consumer properties of the gel.|
|23||2,0||-||1,0||2,0||1,2||4,0||2,0||2,0||0,5||0,05*||0,5||leave||Decrease system stability.|
|26||2,0||-||1,0||2,0||1,2||4,0||2,0||2,0||1,0||0,5||4,0||0,8*||leave||Too pungent smell.|
|* examples beyond the stated limits.|
|Sample||Zone of inhibition of growth of microorganisms mm|
|S. aureus*||E. coli**||Ps. Aeruginosa**|
|Gel, biocide (example 1)||25||30||32|
|Gel, biocide (example 3)||35||27||26|
|Gel, biocide (example 4)||20||22||30|
|* S. aureus is gram - positive microorganisms.|
** - E. coli and Ps. Aeruginosa is a gram-negative microorganisms.
|Sample||Seeding* after a period of time (min)|
|Gel, biocide (example 1)||not OBN.||not OBN.||not OBN.|
|Gel, biocide (example 2)||not OBN.||not OBN.||not OBN.|
|Gel, biocide (example 3)||not OBN.||not OBN.||not OBN.|
|Gel, biocide (example 4)||not OBN.||not OBN.||not OBN.|
|* bacteria of the species E. Coli. 106CFU/cm|
Gel biocidal, including hydroxyethyl cellulose, the connection of polyguanidine, glycerin, Trilon B, glycol, citric acid, perfume is the song and water, characterized in that compounds of polyguanidine use of polyhexamethylene guanidine succinate or succinate poly-(4,9-dioxadodecane) and additionally contains neonol, calcium Pantothenate and chamomile extract in the following ratio, wt.%:
|Succinate, guanidine or|
|Polyethylene glycol PEG-400||1,0-5,0|
|The polyethylene glycol is PEG-40||1,0-1,5|
FIELD: medicine, pharmaceutical industry.
SUBSTANCE: the present innovation deals with developing new medicinal preparations for treating urogenital diseases. The suggested preparation for treating prostatic diseases designed as a suppository that contains the complex of bioregulatory peptides of cattle prostate as a powder with the content of water-soluble peptides being 20%, not less and the foundation, moreover, antimicrobial preparation being the antibiotic or anti-viral preparation, or anti-protozoan preparation, or the mixture of antibiotic and anti-protozoan preparation taken at 1:1 ratio, or the mixture of anti-viral and anti-protozoan preparation taken at 1:1 ratio and the foundation at the following ratio of the components, g/suppository: complex of bioregulatory peptides of cattle prostate 0.05-0.4; antimicrobial preparation 0.7, not more; the foundation - sufficient quantity to obtain the suppository of 2.15-2.35 g weight. The suggested preparation in the form of suppository is of high level of organotropic activity and high level of antimicrobial action that enables to implement etiotropic and pathogenetic therapy of bacterial prostatitis. The innovation provides the action upon an agent and all pathological processes in the affected organ. The innovation enables to treat bacterial prostatic inflammation and that of functionally associated organs.
EFFECT: higher efficiency of therapy.
4 cl, 132 ex, 32 tbl
SUBSTANCE: the resent innovation deals with mixing pertussis component, diphtheric and tetanus anatoxins and sorption of the mixture upon aluminum hydroxide followed by dosing the mixture into the tank, freeze drying the mixture and hermetic sealing of this tank, moreover, as pertussis component one should apply cell-free pertussis anatoxin; as for mixing cell-free pertussis anatoxin, diththeric and tetanus anatoxins it should be carried out simultaneously at availability of phosphate-buffered physiological solution; the mixture obtained should be kept for 1 h at about 4-10°C with subsequent sorption upon aluminum hydroxide; just before freeze drying the mixture should be supplemented with saccharose up to final concentration being 9-10.5%, then the mixture should be mixed and kept at about 4-10C for 1 h. The innovation enables to achieve decreased reactogeneity.
EFFECT: higher efficiency.
2 ex, 4 tbl
FIELD: medicine, pediatrics, obstetrics.
SUBSTANCE: method involves administration of preparations balans-narine-f and calcemin. Carrying out this therapy provides decreasing the content of staphylococci in breast milk, increasing the sterility period of milk based on reducing cellular membranes penetrability and to diminish probability in development complications in treatment of bacteriolactia in nursing women. Invention can be used in treatment of staphylococcus bacteriolactia in women.
EFFECT: improved method of treatment.
1 tbl, 2 ex
FIELD: pharmaceutical chemistry.
SUBSTANCE: invention relates to phenylpyridazine derivative of general formula I , wherein R1 represents C1-C12-alkyl optionally comprising cyclic C3-C6-alkyl structures and optionally substituted by phenyl, which may be substituted by 1-2 halogen atoms; or C1-C12-alkenyl substituted by optionally halogen-substituted phenyl; R2 and R3, independently form each other, represent hydrogen, C1-C12-alkyl, C1-C12-hydroxyalkyl, C1-C12-dihydroxyalkyl, or C1-C12-alkynyl; or R2 and R3, together with adjacent nitrogen atom form 5-6-membered saturated heterocyclic group containing 1-2 nitrogen atoms and optionally oxygen atom, indicated heterocyclic group being optionally substituted by C1-C12-alkyl group, C1-C12-alkoxydicarboxylic group or phenyl-C1-C7-alkyl group; X, Y, and Z, independently form each other, represent hydrogen, halogen, optionally halogen(s)-substituted C1-C12-alkyl, C1-C12-alkoxy, C1-C12-alkylthio, C1-C12-alkylsulfinyl, C1-C12-alkylsulfonyl, or phenyl; and n is a number from 0 to 5; provided that R2 and R3 cannot be simultaneously hydrogen atoms or identical C1-C3-alkyl groups when R1 is benzyl or C1-C3-alkyl group; and salts of compounds I. Foregoing compounds manifest inhibitory activity against production of interleukin IL-1β being well dissoluble in water and characterized by good oral absorption. Invention also relates to therapeutical agent inhibiting production of interleukin 1β, pharmaceutical composition, employment of above-defined compounds, a method for treating disease caused by interleukin 1β production stimulation as well as methods for treating immune system disturbances, inflammatory conditions, ischemia, osteoporosis, or septicemia using above compounds.
EFFECT: expanded therapeutical possibilities.
22 cl, 4 dwg, 2 tbl, 217 ex
FIELD: medicine, gynecology, urology.
SUBSTANCE: on isolating Chlamydia out of infection foci and setting a preliminary diagnosis it is necessary to determine their sensitivity to different etiotropic preparations. Then it is important to sample a patient's blood followed by centrifuging and dividing it into plasma, erythrocytic mass and leukocytic suspension. Removed blood plasma should be substituted with physiological solution, and autoerythrocytes should be returned for a patient in physiological solution intravenously by drops. Autoleukocytic suspension should be activated with laser radiation of He-Ne laser at λ=0.63 mcm. Then it should be supplemented with etiotropic preparation moxyfloxacin to be intravenously injected for a patient. The procedure should be repeated daily for about 10-12 d. The innovation suggested enables to shorten the terms of interrupting the main clinical manifestations of the disease due to a purposeful transport of etiotropic preparation being of higher bacteriological efficiency in leukocytes activated with laser radiation, into primary (urogenital tract) and secondary (extraurogenital) foci of inflammation and, also, decrease side action of chemopreparations due to decreasing their course dosage.
EFFECT: higher efficiency of therapy.
FIELD: veterinary medicine.
SUBSTANCE: vaccine comprises as antigens cell suspension of pure cultures of a streptococcosus pathogen Streptococcus pneumoniae and an enterococcal pathogen Streptococcus fecalis isolated from suspension and prepared from damages organs of dead nutrias from the local epizootic focus wherein these cultures are grown separately in beef-extract broth with glucose with the concentration of microbial cells 4-5 billions in 1 cm3 and mixed in equal ratio. Also, vaccine comprises formalin and aluminum hydroxide in the following ratio of components, wt.-%: cell suspension of pure culture of streptococcosus pathogen Streptococcus pneumoniae isolated from a local epizootic focus in the nutrient medium with a titer 4-5 microbial cells in 1 cm3, 38.0-41.5; cell suspension of pure culture of enterococcal infection pathogen Streptococcus fecalis isolated from a local epizootic focus in the nutrient medium with a titer 4-5 billions of microbial cells in 1 cm3, 38.0-41.5; glucose, 1.0-2.0; formalin, 1.5-2.0, and aluminum hydroxide, the balance. Vaccine is harmful and possesses high specificity and effectiveness.
EFFECT: valuable properties of vaccine.
1 tbl, 5 ex
FIELD: veterinary science.
SUBSTANCE: on finishing the lactation one should inject once antimicrobial preparation "Difumast" per 5 ml into all udder lobes and, additionally, intramuscularly one should inject immunomodulating "preparation "Dostim" at the dosage per 25 ml for the first time, and in 40-45 d - the second. The innovation enables to shorten the quantity of "difumast. The innovation enables to shorten the quantity of "Difumas" injected.
EFFECT: higher accuracy of prophylaxis.
1 tbl;, 1 ex
FIELD: medicine, oncology, biochemistry, antibiotics.
SUBSTANCE: invention relates to variants used in treatment of cancer. Method involves administration in a patient needing with such treatment of the effective dose of 9-amino-6-deoxy-5-hydroxytetracycline or its salt, or 9-nitro-6-deoxy-5-hydroxytetracycline or its salt for aims in treatment of breast cancer, melanoma, myeloma and prostate gland cancer. Invention provides anti-tumor, anti-metastatic effect and the elevating viability index based on inhibition of matrix-destructing metalloproteinase by indicated derivatives of tetracycline and without significant body weight loss.
EFFECT: valuable medicinal properties of derivatives, enhanced effectiveness of treatment.
4 cl, 5 tbl
FIELD: organic chemistry, antibacterial agents.
SUBSTANCE: invention relates to an agent used against acid-resistant microorganisms containing derivative of pyridone carboxylic acid as an active component, its pharmaceutically acceptable salt or its hydrate that elicits high antibacterial activity against Mycobacterium tuberculosis and atypical acid-resistant microorganisms. Invention describes agent used against acid-resistant microorganisms containing compound represented by the following formula (I) its salt or its hydrate as an active component wherein R1 represents cyclic alkyl group comprising 3-6 carbon atoms that can comprise substitute(s) chosen from halogen atom; R2 represents hydrogen atom; R3 represents hydrogen atom; A1 represents incomplete structure represented by the formula (2): wherein X2 represents halogen atom, alkyl group comprising 1-6 carbon atoms or alkoxy-group comprising 1-6 carbon atoms; A1, A2 and A3 form incomplete structure of the formula: in common with carbon atoms combined with them; X1 represents halogen atom; Y represents hydrogen atom; Z represents phenylpiperazine substitute. Invention provides synthesis of pyridone carboxylic acid eliciting high antibacterial activity against Mycobacterium tuberculosis and atypical acid-resistant microorganisms in combination with good pharmacokinetics indices and safety.
EFFECT: valuable biological property of agent.
10 cl, 9 tbl, 10 ex
FIELD: medicine, microbiology, clinical practice.
SUBSTANCE: claimed method includes determination of pectin antioxidant activity (AOA) by chemiluminescent method and on the base of AOA pectin bactericidal properties are evaluated, wherein increased AOA indicates increased bactericidal properties. Method of present invention makes it possible to decrease time of pectin antibacterial property determination from 3 days to 30 min.
EFFECT: method of decreased labor intensity and cost.
1 tbl, 1 dwg
SUBSTANCE: method involves introducing hypertonic sodium chloride solution electrolysis product from electrolizer anode space into abdominal cavity with active chlorine content being equal to 100+10 mg/dm3 as thin layer of liquid in laminar mode in removed exudate volume. Then, electrolysis product of the same solution from electrolizer cathode space is introduced in twice as large removed exudate volume. Peritonitis source is deleted and hypertonic sodium chloride solution electrolysis product is introduced in the same order from electrolizer anode and cathode space.
EFFECT: hindered limphogenic infection distribution process; reduced cancerogenesis risk.
1 dwg, 2 tbl
FIELD: medicine, surgery.
SUBSTANCE: invention relates to a method for treatment of biliary peritonitis. Method involves abdominal cavity sanitation with furaciline, a single intravenous administration of 0.04% sodium hypochlorite solution in a patient as measured 1/10 of volume of circulating blood in patient body, and additional intramuscular administration of 10% alpha-tocopherol solution in 5-6 h as measured 100 mg per 24 h. In the following 24 h sodium hypochlorite and alpha-tocopherol solutions in above given doses are administrated in a single dose with simultaneous administration of cefatoxime in the dose 1 g, 4 times per 24 h and dalargin in the dose 2 mg per 24 h. Method provides normalization of indices of antioxidant system for early period, recovery of cell membranes and penetrability of vessels, decreasing symptoms of endocrine intoxication.
EFFECT: enhanced effectiveness of treatment method.
2 cl, 2 ex
FIELD: pharmaceutical industry and technology.
SUBSTANCE: invention relates to a method for preparing oily extracts from vegetable raw. Method for preparing gum-tree oily extract involves drying branches with 70% of leaves, not less, to the definite moisture index and milled followed by their treatment with electric activated liquid at the definite pH value and in the definite amount of raw mass at stirring and keeping for a definite time. Then prepared mass is wetted with ethyl alcohol, dried to the definite moisture value and extraction is carried out under definite conditions and filtered. Method allows decreasing extraction time and enhancing the yield of total amount of extractive substances.
EFFECT: improved preparing method of extracts.
5 tbl, 9 dwg, 16 ex
FIELD: medicine, pharmacy.
SUBSTANCE: invention relates to therapy of topical suppurative-inflammatory processes. Agent represents an aqueous isoosmotic solution comprising 0.25% of novocaine, 1% of cefazolin sodium salt and 0.77% of sodium chloride. Invention provides the development of optimal conditions for safety mechanical moving of agent in intercellular space under positive hydrodynamic pressure in the permanent state from the injection point in direction to side of deeply localized pathological focus and eliminates the pain component of the inflammatory response.
EFFECT: valuable medicinal properties of agent.
FIELD: veterinary science.
SUBSTANCE: invention proposes a preparation for treatment and prophylaxis of mastitis in cows at onset of the lactation period and in dry cows that comprises the following components, wt.-%: furacrylin, 0.4-0.6; dioxydin, 0.8-1.2; bee wax, 4-6, and sunflower oil, the balance. The preparation is heated to 38-40°C and administrated in cow mammary gland being in damaged and healthy lobules of udder through a nipple duct by using a syringe in the dose 5 ml. Using the preparation provides enhancing therapeutic and prophylactic effectiveness, decreasing labor intensity in treatment and prophylaxis of mastitis in cows at the lactation period and in dry cows.
EFFECT: enhanced and valuable properties of preparation.
3 tbl, 3 ex
FIELD: veterinary science.
SUBSTANCE: the present innovation deals with preventing and treating puerperal endometritis in cows. One should introduce the foam into uterine cavity that appears after interacting with warm water containing 10 U oxytocin and a suppository that includes medicinal substances reduced up to amorphous state. Moreover, components in a suppository are in the following ratio, g (weight%): potassium iodide 1.5 (5.4), ephedrine hydrochloride 0.5 (1.8), furacillin 0.5 (1.8), metronidazole (trichopol) 1.5 (5.4), proserine (prostigmin) 0.06 (0.2), analgin 0.5 (1.8), sodium bicarbonate 9.0 (32.7), potato starch 4.0 (14.6), common salt 1.5 (5.4), citric acid 6.9 (25), calcium stearate 0.3 (1.1), 20%-alcoholic solution of MGD-2 emulsifier or MGD-2-similar one 1.3 (4.8). The innovation provides higher efficiency of local impact of medicinal substances upon pathogenic uterine microflora.
EFFECT: higher efficiency.
1 cl, 1 tbl
FIELD: veterinary medicine.
SUBSTANCE: the present innovation deals with treating and preventing acute and chronic endometritis, vaginitis and balanoposthitis. For this purpose, it is necessary to introduce into an organ's cavity a foam-inducing preparation that contains crystalline substances, that is a foam-inducing suppository or the mixture of substances for its preparing and, also, 100.0 ml 1%-common salt solution heated up to animal body temperature, at the following ratio of suppository components or mixture for its preparing, that provides liquid of alkaline reaction at dissolving, g (weight%): potassium iodide 1.5 (6.3), potato starch 4.0 (16.8), furacillin 0.5 (2.1), metronidazole (trichopol) 1.2 (5.0), analgin 0.5 (2.1), citric acid 6.2 (26.0), sodium bicarbonate (dietary soda) 8.0 (33.7), calcium stearate 0.2 (0.9), calcium carbonate (deposited chalk) 0.5 (2.1), 20%-alcoholic solution of MGD-2 emulsifier or any other similar one 1.2 (5.0). The innovation provides prolonged complex anti-infectious, antiphlogistic, analgesic impact upon animal body, activation of protective and endocrine systems.
EFFECT: higher efficiency of prophylaxis and therapy.
3 cl, 1 dwg, 1 tbl
FIELD: medicine, surgery.
SUBSTANCE: one should apply a polycompositional film onto donor's wounds after autodermoplasty performed. This film contains the following components in weight proportions: chitosan 78.3-89.4; polyvinyl alcohol 9.8-19.8; antibiotic of aminoglycoside group 0.5-2.0; anesthetic 0.1-0.2. It is perforated at tension coefficient being 1:4. The innovation enables to decrease wound's traumatization, improves prophylaxis of suppuration and increases cosmetic effect even at a single application of the film suggested.
EFFECT: higher efficiency of therapy.
FIELD: medicine, stomatology.
SUBSTANCE: for treating one should apply successively 3%-hydrogen peroxide solution followed by 1%-silver nitrate solution. This provides high antiseptic effect in combination with minimal dental dyeing with solver nitrate solution.
EFFECT: higher efficiency.
6 ex, 1 tbl
SUBSTANCE: the innovation deals with manufacturing remedies to prevent wound suppuration based upon polymeric matrix and antimicrobial substance due to mixing components in solution, forming the film to obtain a powder-like medicinal form by providing the safety of antimicrobial activity of medicinal preparation. The obtained remedy is completely biodestructed in the body and requires no repeated operation on its removal after the end of tissue regeneration processes.
EFFECT: higher efficiency of manufacturing.
1 cl, 3 ex, 1 tbl
FIELD: cosmetic medicine.
SUBSTANCE: invention proposes a method for reducing growth of human hair by way of applying dermatologically acceptable topical-administration composition onto skin in amount sufficient to reduce hair growth, which composition contains α-difluoromethylornitin (DFMO) and dermatologically acceptable carrier including at least 4% polyoxyethylene ether to provide percutaneous effects.
EFFECT: enhanced percutaneous power of active ingredient (DFMO).
38 cl, 2 tbl, 6 ex