Biocidal gel

FIELD: pharmaceutical industry, in particular biocide agent.

SUBSTANCE: claimed gel includes hydroxyethyl cellulose, polyguanidine compound (e.g. polyhexamethylene guanidine succinate or poly-(4,9-dioxadodecan guanidine) succinate), glycerol, trilon B, polyethylene glycol, citric acid, perfume composition, and water. Additionally it contains neonol, calcium pantothenate and chamomile extract in specific component ratio.

EFFECT: gel with increased biocidal action.

3 tbl, 3 ex

 

The invention relates to medicine and can be used in surgery, obstetrics, emergency stations, hospitals and other medical institutions. The gel can also be used in the food industry, public utilities, public power and in everyday life.

This vast and varied scope of the inventive gel due to its properties and effect: it has a disinfectant, antibacterial, antiseptic, antibacterial properties.

Known gel for antiseptic treatment of skin, containing in its composition as an antiseptic Miramistin (U.S. Pat. Of the Russian Federation No. 2185157, CL AC 31/14, 2000).

The disadvantages of this gel is that applied Miramistin is a derived group of biguanidine and not have a prolonged effect. The scope of Miramistin limited. At the same time, the antibacterial activity of Miramistin is extremely low.

The closest solution to the present invention is a hygienic gel of the following composition, wt.%:

- methylcellulose or hydroxyethylcellulose0,3-3,0
- glycerin0.5 to 10.0
- citric acid0,5-3,0
- cremophor0,1-5,0
- Trilon B - sodium salt
ethylenediaminetetraacetic acid0,1-2,0
- perfume0,05-0,5
connection of polyguanidine or
synergistic mixture of Quaternary
ammonium compounds
dimethylbenzylamine sulfate0,5-3,0
- peg0,1-1,0
vitamin component0,1-2,0
- waterrest

In the gel hygienic as connection polyguanidine use phosphate poly-(4,9-dioxadodecane), or citrate/formate, guanidine, or gluconate/benzoate, guanidine, or chloride, guanidine (U.S. Pat. Of the Russian Federation No. 2225202 B. I. No. 7, 2004).

However, the known gel has a number of disadvantages: the use of cremophor a negative effect on the technological properties of the gel, namely deteriorating penetration of the gel into the skin. In addition, the presence of cremophor in the composition of the gel leads to a deterioration of the homogeneity of the composition during storage. It is also noted that the presence of dimethylbenzylamine sulfate impedes the penetration of the gel through to the, because of what decreases the bactericidal effect.

The objective of the invention is to increase the biocidal effect and reduce side effects from the use of gel.

To solve the problem in the gel biocidal, including hydroxyethyl cellulose, the connection of polyguanidine, glycerin, cremophor, Trilon B, glycol, citric acid, perfume composition and water quality of the connection of polyguanidine use of polyhexamethylene guanidine succinate or succinate poly-(4,9-dioxadodecane), and it additionally contains neonol, calcium Pantothenate and chamomile extract in the following ratio, wt.%:

Hydroxyethylcellulose0,5-2,0
Succinate, guanidine
or succinate poly-(4,9-dioxadodecane)0,3-3,0
Glycerin1,0-10,0
Polyethylene glycol PEG-4001,0-5,0
The polyethylene glycol is PEG-401,0-1,5
Calcium Pantothenate0,1-2,0
Neonol1,0-3,0
Trilon B0,1-2,0
Chamomile extract0,5-2,0
Citric acid0,1-5,0
Perfume0,03-0,5
Water gilliaunarest

The invention is illustrated as follows.

Highly potent compounds of polyguanidine have a broad spectrum of biocidal activity: antibacterial, fungicidal, virucidal. They have low toxicity, does not penetrate intact skin (IV class of danger), saved it for a long time, protecting it from exposure to the environment.

As compounds of polyguanidine use succinate pgmg (disubstituted) or succinate poly-(4,9-dioxadodecane) (disubstituted).

These compounds possess a wide spectrum of biocidal activity.

Succinate pgmg (disubstituted) has the following structural formula:

n=30-50

Succinate poly-(4,9-dioxadodecane) (disubstituted) has the following structural formula:

n=30-50

They can be obtained as follows.

The action of alcohol CON from pgmg chloride or chloride poly-(4,9-dioxadodecane) are removed completely ions Cl-and alcohol solution of the obtained bases polyguanidine are alcoholic solution of succinic acid. The alcohol is distilled off and get anhydrous solid salt succinate pgmg or succinate p is whether-(4,9-dioxadodecane) (disubstituted).

Succinic acid, which is part of succinate pgmg and poly-(4,9-dioxadodecane)is bioactive natural compound that is present in the human body and therefore salt of succinic acid necessary for human body. Biocidal effect of the gel is increased due to the fact that succinate pgmg and succinate poly-(4,9-dioxadodecane) contributes to a more prolonged biocidal effect of the gel after application to the skin and penetration.

Used in the composition of the gel as the gel-forming component hydroxyethylcellulose is a powder of white or grayish color with the main component content not less than 97 wt.% (THE 6-05-1857-87).

Glycerin (TU-6259-25) is a transparent liquid with a basic substance content not less than 99%. Glycerin - enveloping and astringent component. Provides skin soothing and astringent effect, promotes penetration into the skin active agents, activates metabolic processes. The use of glycerol increases the efficacy of antiseptic.

Neonol - non-ionic surfactant is in Russia, comes in the form of 100% viscous water-soluble liquid (TU 6-05-1857-87), detergent for use in shampoos, gels for body and other Structural formula is as follows:

R=C4-C10n=5-20

Polyethylene glycol (PEG-400) (TU-6-00205601.083-2000) is a viscous or waxy substance, soluble in organic solvents, sparingly in water, precipitates from aqueous solutions at temperatures close to 100°C. the polyethylene Glycol is used as a thickener and binder. PEG-40 - viscous waxy substance is hydrogenated castor oil.

When applying guanidino antiseptics in gel PEG soften their effect on the skin and enhances the biocidal effect. PEG is able to penetrate into the subcutaneous tissue and transported there drugs, as well as to improve the stability of the composition over time, to improve wetting and regenerative ability of the gel. In addition, it also improves the penetrating ability of the gel, which is beneficial to its medicinal properties.

Calcium Pantothenate calcium salt of D-(+) Pantothenic acid. Pharmacological action replenishes the deficiency of Pantothenic acid, softens the skin, facilitates the absorption of the components of the gel in particular, succinate pgmg and succinate poly-(4,9-dioxadodecane).

Trilon B (GOST-10652) is a sodium salt of ethylenediaminetetraacetic acid, complex III is an organic chelate forming compound, crystallises is e, soluble in water, ethanol and most organic solvents.

Used as a titrant in complexometry, for division and separation of metal ions to dissolve and prevent the formation of various sediments, caused, for example, water hardness, for excretion from the body of toxic metals, stabilization of food, water softener, as components of detergents.

Citric acid (GOST-3652) is a colorless crystals with monoclinic lattice. Solubility in water, % (temperature, °): 59,2 (20), 84 (100); molecular weight: 192,2. Apply the gel as complexing agents and pH regulator. Shows the properties of polybasic carboxylic acids, forms three series of salts and esters (citrates) carboxyl groups.

Citric acid and its salts are used in the food industry as flavouring substances as components of the buffer solutions. To prevent fats from spoiling, in the pharmaceutical industry as components of many drugs, sodium citrate is an anticoagulant.

Chamomile extract is used in the gel as a component with anti-allergic and anti-inflammatory action.

Fragrance composition is an organic liquid with a characteristic party Arnim smell. Use different songs with fruity floral, fruit and herbal, spicy, pine aromas. The most preferred odorants JSC "Balm" (Russia, Nizhny Novgorod).

As the basis in the cream composition using distilled water (GOST 609-84).

The quantitative content of the components of the gel, recommended by us, is optimal and selected on the basis of numerous experiments.

Table 1 presents data confirming the optimality of the claimed ratios of the components, as well as the data that go beyond the stated ratios.

The process of making gel disinfectant includes preparation of raw materials and equipment, weighing components, the dispersion of hydroxyethyl cellulose in glycerol in the presence of neonols for 1.5-2 hours at a temperature of 30-40°with periodic mixing.

Separately preparing an aqueous solution getcomponent by dissolving in water.

Aqueous solutions of PEG-400 and PEG-40 cook with constant stirring. Separately prepared aqueous solution of calcium Pantothenate and an aqueous solution of the trylon.

Almost the preparation of a disinfectant gel as follows: in the reactor pour the prepared solution of hydroxyethyl cellulose in glycerol and surfactant, an aqueous solution of PEG-400 and PEG-40, an aqueous solution of descampe the NTA, aqueous solutions of calcium Pantothenate and trylon B, the perfume composition. After mixing, add citric acid to bring the pH of the prepared mixture to the desired neutral level.

The resulting mass is diluted with water in accordance with the recipe, continuously stirring at a temperature of 25-30°to obtain a homogeneous solution.

Testing of antimicrobial activity designed disinfectant gel used 2 ways. By the first method defined zone of inhibition of microorganisms by diffusion of the analyte in a dense nutrient medium (Fedorov Y.A., Root NR. "Microbiological research methods in dental hygiene products" in the book. "Basics of oral hygiene". L.: "Medicine", 1973, p.3-215).

The Petri dish was filled with 20 ml of a dense nutrient medium. Prepared 24 hours a microbial suspension of test microorganism with a concentration of 5×105CFU/ml and applied to 1 ml of the suspension on the surface of the medium. Was dried for 30 min, and then in a nutrient medium made holes with a diameter of 6 mm, which was made samples developed disinfectant gel. The number of holes should not exceed 6 per Petri dish. For 30 min in Petri dishes kept at room temperature (20°C), then they were placed in the incubator for 24-48 hours in temp is the temperature 37° And after a specified period of time measured the zone of inhibition of microbial growth, including the diameter of the hole. The size of zones of inhibition of growth of test microorganisms was judged on the antimicrobial activity of the test substance, although it should be considered that the diffusion rate of the polymeric disinfectants lower rate of diffusion of low molecular weight disinfectants.

To obtain reliable test results of each analyte performed in 2-3 series of replications.

The second way to test antimicrobial activity of gel consists of determining the minimum time required for complete suppression of the growth and development of test microorganisms.

In the test substance was added to a suspension of the test organisms in the amount of 106CFU/ml in a test tube pour 1 ml of the test substance. The tubes were shaken and intervals 2-5-10 and 30 minutes did the sowing on the solid nutrient medium. Petri dishes were placed in 24 to 48 hours in a thermostat at 37°C. After the expiration of that period were counting the number of grown colonies and this indicator was judged on the bactericidal activity of the test substance.

The results of these studies are presented in table 2.

From the presented data in table 2 it follows that a more sensitive test means b is Li gram-positive organisms (S. aureus). All test tools formed a zone of stunted growth from 22 to 35 mm, which is sufficiently severe or high activity. Draws attention to the high activity of the preparation according to example 3 to gram-negative microorganisms of the species E. coli and Ps. aeruginosa.

When determining the speed of the manifestations of antimicrobial activity test tools the following results were obtained (table 3).

From the presented data in table 3 it follows that the antibacterial effect of the developed formulations is developing very quickly. After 2 min of contact in any case not found living microflora after contamination of the nutrient medium by Escherichia coli.

To verify the validity of disinfectant gel were tested on volunteers in accredited specialized Center. Marked highly effective action of the gel as a disinfectant on the skin.

There was also a certain therapeutic effect of gel in the treatment of purulent wounds and ulcers. Marked healing effect without the formation of superficial scars.

Special experiments showed that succinate pgmg and succinate poly-(4,9-dioxadodecane) unlike pgmg hydrochloride pgmg-phosphate active against spore-forming bacteria. This extends the application of the gel.

In the result of the verification is built Toxicological studies have established the that the gel is completely harmless, irritating and allergenic action.

Example 1. Content, wt.%:

Hydroxyethylcellulose1,0
Succinate poly-(4,9-
dioxadodecane)2,0
Glycerin5,0
PEG-4005,0
PEG-401,0
Calcium Pantothenate2,0
Neonol2,0
Trilon B1,0
Chamomile extract1,0
Citric acid3,0
Perfume0,3
Waterrest

Gel well onto skin, consumed sparingly and quickly absorbed. Promotes the protection and restoration of the damaged structures of the skin. On the skin surface is formed an invisible polymer film that protects the skin and provides its sterility until then, until it is washed off.

The gel can be used for processing of the arms trade workers, food industry workers, and also the hands of the surgeon before the operation. After drying of the gel on the skin surface has reached what is bactericidal effect of gloves.

Example 2. Content, wt.%:

Hydroxyethylcellulose2,0
Succinate pgmg0,3
Glycerin10,0
PEG-4001,0
PEG-401,5
Calcium Pantothenate0,1
Neonol3,0
Trilon B0,1
Chamomile extract2,0
Citric acid5,0
Perfume0,03
Waterrest

When processing the feet of the obtained gel eliminates the smell of sweat, protects against infection by a fungus that kills pathogens. The gel can be used for processing Shoe after wearing.

Example 3. Content, wt.%:

Hydroxyethylcellulose0,5
Succinate pgmg3,0
Glycerin1,0
PEG-4003,0
PEG-401,2
Calcium Pantothenate1,0
Neonol1,0
Trilon B 2,0
Chamomile extract0,5
Citric acid0,1
Perfume0,5
Waterrest

This composition can be used for healing of wounds, abrasions, sores, burns of various origins, wet eczema, fungal infections of the skin. Especially it is effective when the dressing with the gel on the festering wound. After treatment gel purulent wounds and wounds with uneven edges were observed scarring.

It should be noted that the disinfectant gel in all of these examples, as well as examples of table 1 starts instantly and biocidal effect persists for at least 5 hours.

The safety of the gel and its homogeneous composition determines mainly the active substance - succinate pgmg or succinate poly-(4,9-dioxadodecane). The shelf life of the gel reaches 5 years. During this period, its color does not change, and disinfectant properties are not lost.

td align="center"> 9
Table 1
No. AveHydroxyethylcelluloseSuccinate pgmgSuccinate poly-(4,9-dioxadodecane-guanidinePEG-400PEG-40Glycerin Calcium PantothenateNeonolTrilon BChamomile extractCitric acidPerfumeWaterNote
1234567891011121314
11,0-2,05,01,05,02,02,01,01,03,00,3leaveHomogeneous transparent gel with prolonged disinfectant effect.
22,00,3-1,01,510,00,13,00,12,05,00,03leave
30,53,0-3,01,21,01,01,02,00,50,10,5leaveSupports the natural moisture balance in the skin.
41,5-1,02,01,54,02,03,01,01,54,00,5leave
50,2*1,0-2,01,24,02,02,01,00,54,00,5leaveLiquid gel.
62,2*1,0-2,01,24,02,02,01,00,54,00,5leaveToo thick gel.
72,0-0,1*2,01,24,02,02,01,00,54,00,5leaveNot enough disinfectant effect.
82,0-3,2*2,01,24,02,02,01,00,54,00,5leaveToo high costs of disinfectant.
2,0-1,00,7*1,24,02,02,01,00,54,00,5leaveLiquid gel.
102,0-1,05,3*1,24,02,02,01,00,54,00,5leaveThe gelling of the gel.
112,0-1,02,01,0*4,02,02,01,00,54,00,5leaveDeterioration requirements. properties of the gel.
122,0-1,02,01,7*4,02,02,01,00,54,00,5leaveThe increase in viscosity of the gel.
132,0-1,02,01,20,8*2,02,01,00,54,00,5leavePoor absorption into the skin.
142,0-1,02,01,210,3*2,02,01,00,54,00,5leaveThe gelling of the gel.
152,0-1,02,01,24,00,05*2,01,00,54,00,5leaveThe decrease in biological activity.
162,0-1,02,01,24,02,1*2,01,00,54,00,5leaveUnnecessarily high consumption of the component.
172,0-1,02,01,24,02,00,5*1,00,54,00,5leaveThe deterioration of the stability of the gel.
182,0-1,02,01,24,02,03,1*1,00,54,00,5 leaveLeads to the formation and loss of antimicrobial activity.
192,0-1,02,01,24,02,02,00,05*0,54,00,5leaveThe deterioration of the stability of the gel.
202,0-1,02,01,24,02,02,02,1*0,54,00,5leaveThe decrease in antimicrobial activity.
212,0-1,02,01,24,02,02,01,00,25*4,00,5leaveThe reduction of consumer properties of the gel.
222,0-1,02,01,24,02,02,01,02,2*4,00,5leave
232,0-1,02,01,24,02,02,0 0,50,05*0,5leaveDecrease system stability.
242,0-1,02,01,24,02,02,01,00,55,1*0,5leave
252,0-1,02,01,24,02,02,01,00,54,00,01*leaveCons. smell.
262,0-1,02,01,24,02,02,01,00,54,00,8*leaveToo pungent smell.
* examples beyond the stated limits.

Gel, biocide (example 2)
Table 2
SampleZone of inhibition of growth of microorganisms mm
S. aureus*E. coli**Ps. Aeruginosa**
Gel, biocide (example 1)253032
322528
Gel, biocide (example 3)352726
Gel, biocide (example 4)202230
* S. aureus is gram - positive microorganisms.

** - E. coli and Ps. Aeruginosa is a gram-negative microorganisms.

Table 3
SampleSeeding* after a period of time (min)
23060
Gel, biocide (example 1)not OBN.not OBN.not OBN.
Gel, biocide (example 2)not OBN.not OBN.not OBN.
Gel, biocide (example 3)not OBN.not OBN.not OBN.
Gel, biocide (example 4)not OBN.not OBN.not OBN.
* bacteria of the species E. Coli. 106CFU/cm

Gel biocidal, including hydroxyethyl cellulose, the connection of polyguanidine, glycerin, Trilon B, glycol, citric acid, perfume is the song and water, characterized in that compounds of polyguanidine use of polyhexamethylene guanidine succinate or succinate poly-(4,9-dioxadodecane) and additionally contains neonol, calcium Pantothenate and chamomile extract in the following ratio, wt.%:

Hydroxyethylcellulose0,5-2,0
Succinate, guanidine or
succinate poly-(4,9-dioxadodecane)0,3-3,0
Glycerin1,0-10,0
Polyethylene glycol PEG-4001,0-5,0
The polyethylene glycol is PEG-401,0-1,5
Calcium Pantothenate0,1-2,0
Neonol1,0-3,0
Trilon B0,1-2,0
Chamomile extract0,5-2,0
Citric acid0,1-5,0
Perfume0,03-0,5
Distilled waterRest



 

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2 cl, 2 ex

FIELD: pharmaceutical industry and technology.

SUBSTANCE: invention relates to a method for preparing oily extracts from vegetable raw. Method for preparing gum-tree oily extract involves drying branches with 70% of leaves, not less, to the definite moisture index and milled followed by their treatment with electric activated liquid at the definite pH value and in the definite amount of raw mass at stirring and keeping for a definite time. Then prepared mass is wetted with ethyl alcohol, dried to the definite moisture value and extraction is carried out under definite conditions and filtered. Method allows decreasing extraction time and enhancing the yield of total amount of extractive substances.

EFFECT: improved preparing method of extracts.

5 tbl, 9 dwg, 16 ex

FIELD: medicine, pharmacy.

SUBSTANCE: invention relates to therapy of topical suppurative-inflammatory processes. Agent represents an aqueous isoosmotic solution comprising 0.25% of novocaine, 1% of cefazolin sodium salt and 0.77% of sodium chloride. Invention provides the development of optimal conditions for safety mechanical moving of agent in intercellular space under positive hydrodynamic pressure in the permanent state from the injection point in direction to side of deeply localized pathological focus and eliminates the pain component of the inflammatory response.

EFFECT: valuable medicinal properties of agent.

2 ex

FIELD: veterinary science.

SUBSTANCE: invention proposes a preparation for treatment and prophylaxis of mastitis in cows at onset of the lactation period and in dry cows that comprises the following components, wt.-%: furacrylin, 0.4-0.6; dioxydin, 0.8-1.2; bee wax, 4-6, and sunflower oil, the balance. The preparation is heated to 38-40°C and administrated in cow mammary gland being in damaged and healthy lobules of udder through a nipple duct by using a syringe in the dose 5 ml. Using the preparation provides enhancing therapeutic and prophylactic effectiveness, decreasing labor intensity in treatment and prophylaxis of mastitis in cows at the lactation period and in dry cows.

EFFECT: enhanced and valuable properties of preparation.

3 tbl, 3 ex

FIELD: veterinary science.

SUBSTANCE: the present innovation deals with preventing and treating puerperal endometritis in cows. One should introduce the foam into uterine cavity that appears after interacting with warm water containing 10 U oxytocin and a suppository that includes medicinal substances reduced up to amorphous state. Moreover, components in a suppository are in the following ratio, g (weight%): potassium iodide 1.5 (5.4), ephedrine hydrochloride 0.5 (1.8), furacillin 0.5 (1.8), metronidazole (trichopol) 1.5 (5.4), proserine (prostigmin) 0.06 (0.2), analgin 0.5 (1.8), sodium bicarbonate 9.0 (32.7), potato starch 4.0 (14.6), common salt 1.5 (5.4), citric acid 6.9 (25), calcium stearate 0.3 (1.1), 20%-alcoholic solution of MGD-2 emulsifier or MGD-2-similar one 1.3 (4.8). The innovation provides higher efficiency of local impact of medicinal substances upon pathogenic uterine microflora.

EFFECT: higher efficiency.

1 cl, 1 tbl

FIELD: veterinary medicine.

SUBSTANCE: the present innovation deals with treating and preventing acute and chronic endometritis, vaginitis and balanoposthitis. For this purpose, it is necessary to introduce into an organ's cavity a foam-inducing preparation that contains crystalline substances, that is a foam-inducing suppository or the mixture of substances for its preparing and, also, 100.0 ml 1%-common salt solution heated up to animal body temperature, at the following ratio of suppository components or mixture for its preparing, that provides liquid of alkaline reaction at dissolving, g (weight%): potassium iodide 1.5 (6.3), potato starch 4.0 (16.8), furacillin 0.5 (2.1), metronidazole (trichopol) 1.2 (5.0), analgin 0.5 (2.1), citric acid 6.2 (26.0), sodium bicarbonate (dietary soda) 8.0 (33.7), calcium stearate 0.2 (0.9), calcium carbonate (deposited chalk) 0.5 (2.1), 20%-alcoholic solution of MGD-2 emulsifier or any other similar one 1.2 (5.0). The innovation provides prolonged complex anti-infectious, antiphlogistic, analgesic impact upon animal body, activation of protective and endocrine systems.

EFFECT: higher efficiency of prophylaxis and therapy.

3 cl, 1 dwg, 1 tbl

FIELD: medicine, surgery.

SUBSTANCE: one should apply a polycompositional film onto donor's wounds after autodermoplasty performed. This film contains the following components in weight proportions: chitosan 78.3-89.4; polyvinyl alcohol 9.8-19.8; antibiotic of aminoglycoside group 0.5-2.0; anesthetic 0.1-0.2. It is perforated at tension coefficient being 1:4. The innovation enables to decrease wound's traumatization, improves prophylaxis of suppuration and increases cosmetic effect even at a single application of the film suggested.

EFFECT: higher efficiency of therapy.

2 ex

FIELD: medicine, stomatology.

SUBSTANCE: for treating one should apply successively 3%-hydrogen peroxide solution followed by 1%-silver nitrate solution. This provides high antiseptic effect in combination with minimal dental dyeing with solver nitrate solution.

EFFECT: higher efficiency.

6 ex, 1 tbl

FIELD: pharmaceutics.

SUBSTANCE: the innovation deals with manufacturing remedies to prevent wound suppuration based upon polymeric matrix and antimicrobial substance due to mixing components in solution, forming the film to obtain a powder-like medicinal form by providing the safety of antimicrobial activity of medicinal preparation. The obtained remedy is completely biodestructed in the body and requires no repeated operation on its removal after the end of tissue regeneration processes.

EFFECT: higher efficiency of manufacturing.

1 cl, 3 ex, 1 tbl

FIELD: cosmetic medicine.

SUBSTANCE: invention proposes a method for reducing growth of human hair by way of applying dermatologically acceptable topical-administration composition onto skin in amount sufficient to reduce hair growth, which composition contains α-difluoromethylornitin (DFMO) and dermatologically acceptable carrier including at least 4% polyoxyethylene ether to provide percutaneous effects.

EFFECT: enhanced percutaneous power of active ingredient (DFMO).

38 cl, 2 tbl, 6 ex

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