Method for treating urogenital chlamydial infection
FIELD: medicine, gynecology, urology.
SUBSTANCE: on isolating Chlamydia out of infection foci and setting a preliminary diagnosis it is necessary to determine their sensitivity to different etiotropic preparations. Then it is important to sample a patient's blood followed by centrifuging and dividing it into plasma, erythrocytic mass and leukocytic suspension. Removed blood plasma should be substituted with physiological solution, and autoerythrocytes should be returned for a patient in physiological solution intravenously by drops. Autoleukocytic suspension should be activated with laser radiation of He-Ne laser at λ=0.63 mcm. Then it should be supplemented with etiotropic preparation moxyfloxacin to be intravenously injected for a patient. The procedure should be repeated daily for about 10-12 d. The innovation suggested enables to shorten the terms of interrupting the main clinical manifestations of the disease due to a purposeful transport of etiotropic preparation being of higher bacteriological efficiency in leukocytes activated with laser radiation, into primary (urogenital tract) and secondary (extraurogenital) foci of inflammation and, also, decrease side action of chemopreparations due to decreasing their course dosage.
EFFECT: higher efficiency of therapy.
A method for the treatment of urogenital chlamydial infection relates to the field of medicine.
According to official data in recent years in Russia every year there are more than 350,000 patients with urogenital chlamydial infection. But in fact these patients much more. This is largely due to widespread self-medication, lack of registration of patients in commercial diagnostic and treatment centers, and private medical practitioners. Of particular importance are common (generalized) form of this infection, which is caused by hematogenous introduction of the pathogen from the primary foci of infection in the urogenital tract to other organs and tissues, which ultimately leads to the formation of a secondary (extravagantly) foci of infection. The most typical forms of this infection are: disease Reiter, vasculitis, serous meningitis and other Treatment of such patients is not well developed, since it is difficult to find effective treatments for one or another causal drug and rational way of its introduction. The complex factors that result in low efficiency of etiotropic therapy, include: the use of etiotropic drugs without taking into account sensitivity to them chlamydia, insufficient dose and duration of treatment, failure to comply with the control of the cure. So WESM what is important to search for and create new ways technologies that provide adequate and effective therapy for patients with common forms of urogenital chlamydial infection.
Known methods of treatment of urogenital chlamydial infection, such as disease Reiter. Used to treat with traditional combination therapy: massage of the prostate gland No. 10 in a day, ultrasound therapy in the crotch in the area of projection of the prostate gland No. 10, bacteriotherapy (batteryoperated - sporobacterin 1/2 bottle 2 times a day), Essentiale Forte 2 capsules 3 times a day during meals, trental 100 mg daily. Antibacterial therapy of such patients shall be held within 18 days, see "journal of 3abolevaniya, sexually transmitted diseases, No. 1, 1998, s-24.
The disadvantages of this treatment are: lack of effective treatment, long term treatment, resulting in no observed final rehabilitation of the affected tissues from chlamydia, not completely stymied clinical syndromes as immediately after treatment and in long-term follow-up period (2-3 months) after end of treatment.
The closest solution to the proposed method of treatment of urinary Gediminas infection is a method of treating urogenital hladimioz indicated in the journal "Herald of the Russian Academy of medical is their Sciences, No. 10, 2003, p.29-34, article J.V. Lobina, NR. The Volzhanin, A.L. the Paznyak New in the diagnosis and treatment of common forms of urinary hladimioz", and providing a diagnosis to the patient, etiotropic therapy taking into account the sensitivity of the selected chlamydia to etiotropic drugs. In patients with mixed infection, the choice of antibiotic is based on sensitivity to it all microbes is associated with. In the treatment of advanced were used chemotherapy drugs (metronidazole, delagil, makmiror), which strengthened antichlamydial and antimycoplasmal activity such modern etiotropic drugs as jozamitsin and ofloxacin. Patients were treated with a 20-day course of therapy was continuous.
The disadvantages of the above method of treatment are: the use of monoethylether was not sufficiently effective, the biological effectiveness 3 months after treatment was only 66.7%of clinical effectiveness is also insufficient, because there was a great period of treatment and lack of effective treatment secondary extravagantly foci of infection.
The technical result of the treatment of urogenital chlamydial infection are: to improve the effectiveness of treatment by reducing the terms of the relief of the main clinical manifestations of the disease, the bacteria is of bacteriological methods of efficiency thanks to a complete renovation of primary and secondary foci of infection after the end of treatment (12-13 days) and in the more distant the observation period (2-3 months), reducing the dose rate of the use of causal drug 1/3, reducing the possibility of adverse effects of therapy.
This result is achieved by the fact that in the treatment of urogenital chlamydial infections, including diagnosis, selection of chlamydia from different foci of infection of the patient, determination of their sensitivity to different causal preparations and creation in the urogenital tract and in extravagantly foci of concentration of the selected drug, after the blood of the patient sequentially separated by centrifugation taken the blood of the patient on plasma, erythrocyte mass and leukocyte suspension, then the remote plasma substitute saline, autoerythrocyte physiological solution is returned to the patient intravenously, and autolackiererei suspension activate the laser radiation, then it add prescribed to treat causal drug antichlamydial activity and is administered intravenously to the patient, repeating the treatment every day for 10-12 days, and the activation of the leukocyte suspension is conducted by radiation from a He-Ne laser with λ=0.63 µm and 10 MW, and antichlamydial causal drug take from fluoroquinolone series, e.g. the R moxifloxacin.
The essence of the invention is expressed in a set of key characteristics, sufficient to achieve provided by the invention a technical result.
The essential features of the method of treatment, coinciding with the known characteristics of the prototype are: A diagnosis; B - allocation of chlamydia from different foci of infection of the patient and determining their sensitivity to different etiotropic drugs; creating in the urogenital tract and in extravagantly foci of concentration of the selected drug.
Salient features of the proposed method of treatment are: G - after collection of blood in a patient sequentially separated by centrifugation taken the blood of the patient on plasma, erythrocyte mass and leukocyte suspension; D - remote blood plasma substitute saline; E - autoerythrocyte physiological solution is returned to the patient intravenously; W - autolackiererei suspension activate the laser radiation; And in the activated suspension type assigned to etiotropic treatment drug with antichlamydial activity and is administered intravenously to the patient; It is a medical procedure is repeated daily for 10-12 days.
Private distinctive signs: L - activation of the leukocyte suspension is conducted by radiation from a He-N laser λ =0.63 µm and 10 MW; M - antichlamydial causal drug take from fluoroquinolone series, such as moxifloxacin.
A method for the treatment of urogenital chlamydial infection include: diagnosis, selection of chlamydia from different foci of infection of the patient. The primary focus - the genitourinary tract is a urethritis, a prostatitis. Secondary foci - extravagantly - this can be a joints - arthritis, synovitis, eye - conjunctivitis, himaruya sinus. Then determine the sensitivity of chlamydia to various etiotropic drugs, and ultimately create in the urogenital tract and in extravagantly foci of high concentrations of selected causal drug. The sensitivity of chlamydia to etiotropic drugs define the culture method in advance before the treatment. For the diagnosis of urogenital chlamydial infection, and to determine the antibiotic resistance in selected chlamydia apply their cultivation on transplantable cell culture. For this purpose use a variety of cell types, with high sensitivity for chlamydia: McCoy, Hela-229, L-229, KSS-221. Preference is thus given to the mixed cell lines from a number of recommended and sent to the demands of medical institutions. When this method is used mixed transplantable cultures the cells LLC+MK2+L929+BNK-21c with pre-reduced resistance. When treating patients with urogenital chlamydial infection after collection (exfuze) venous blood of the patient consistently by centrifugation at refrigerated centrifuge RS-6 for 10 minutes at different speeds share the collected plasma, erythrocyte mass and leukocyte suspension. Remote toxic blood plasma substitute in adequate quantities saline solution of 0.85% sodium chloride (plazmozameschayuschie solution). Autoerythrocyte physiological solution is returned to the patient intravenously. Autolackiererei suspension activate the laser radiation from a He-Ne laser with λ=0.63 µm and 10 MW for 10-15 minutes, then it (the suspension) add prescribed to treat atitray drug with antichlamydial activity (sorbirovaniya) and injected intravenously to the patient. As causal of the drug in this disease the most effective fluoroquinolone, for example, moxifloxacin 400 mg daily. Procedures for the above-stated method for the treatment of urogenital chlamydial infection (also called therapeutic plasma leukocytapheresis with antibiotic therapy) spend every day at the same time of day for 10-12 days.
As an example, the particular application of this method can lead to the treatment of patients with disease Reiter (16 che who ovec). Patients were between 18 and 35 years, men without other comorbidity. Treatment effectiveness was assessed with the terms of the relief of the main clinical syndrome (a syndrome common infectious intoxication, artrologicheskoy syndrome, conjunctivitis, severity and duration of urethritis and prostatitis). Introduction causal drug moxifloxacin 400 mg was administered every day for 12 days. As criteria of cure patients from chlamydial infection were used results from the application of the following laboratory methods: polymerase chain reaction, direct immunofluorescence method - PIF, as well as the culture method. Dynamic clinical and laboratory monitoring of patients and survivors was conducted in terms of 13, 23 and 60 days from the start of treatment. In patients with disease Reiter, whose treatment was carried out with the help of this method, in a shorter time stoped main clinical syndromes or immediately after completion of treatment. Research conducted in the remote observation period (60 days from start of treatment)showed no clinical signs (symptoms) of the disease. This method of treatment of urogenital chlamydial infection contributed to the reduction of time (20 days) relief of the main clinical syndromes in patients with disease Reiter and dormancy the hall a high clinical and bacteriological efficacy compared with patients in the traditional way.
Examples of the treatment of patients under the proposed method are specified in appendices 1 and 2.
The use of the invention "Method of treatment of urogenital chlamydial infection compared with the traditional method of treatment is the prototype allows to increase the effectiveness of the treatment by reducing the terms of the relief of the main clinical manifestations of the disease, to improve bacteriological efficacy due to the complete renovation of primary and secondary foci of infection after the end of treatment, and in the more distant the observation period. A reduction in dose rate used causal agent as well as reducing the possibility of side effects. A method of treating substantially (20 days) reduces edema of the main clinical manifestations of the disease, which indicates a high clinical efficacy compared with conventional intravenous antibiotic therapy. Due to the directed transport of causal drug activated by laser radiation the cells in the primary (urinary tract) and secondary (extravagantly) inflammation this method of treatment has higher bacteriological efficacy and lack of side effects. A complete restoration of both primary and secondary foci of infection on udaetsya immediately after the application of the developed method of treatment for 13 days from the start of treatment. Ultimately, the proposed method of treatment allows to achieve rehabilitation of the affected organs from chlamydia and remote observation period (60 days from start of treatment). A method of treating urogenital chlamydial infection, you can create primary and secondary foci of chlamydial infection effective concentration causal drug moxifloxacin, which allows to reduce his dose rate at 30%, and so to avoid the development of side effects during long use. When this is achieved, the economic effect (reduced course the cost of treatment). The treatment efficiency of this method can be recommended for widespread use in medical practice.
Example 1. Patient I., aged 22, he sought medical care in the clinic of infectious diseases about the "fever of unknown etiology", considers himself ill for 3 years, when there were subterrania temperature, General weakness, malaise, recurrent pain and swelling in the joints, dysuric phenomena, disorders of sexual function. Twice was treated about urogenital chlamydia (used antibacterial therapy, bacteriotherapy, immunotherapy, enzyme therapy). However, to achieve full rehabilitation of the urinary tract from chlamydia failed. In Ogloblin the survey in the clinic using molecular-biological methods (PCR), the method of direct immunofluorescence (DIF) and indirect immunofluorescence reaction (NRIF), the culture method C.trachomatis was discovered in soskoba material from the urethra, the prostate gland secretion, centrifugate urine and ejaculate (primary lesions). In addition, this pathogen (chlamydia) was detected in the mucosa of the nasopharynx, conjunctiva, swabs from the maxillary sinus, in lacokanostra venous blood and synovial fluid of affected joints (secondary lesions). According to NIF titles chlamydial antibodies in the serum was 1:32. Research on other infectious, sexually transmitted diseases and pathogens of respiratory and intestinal (yersiniosis) infections were negative. On the basis of the results of examination the patient was put the final diagnosis of the disease Reiter. In the following, using the culture method was found high sensitivity of chlamydia to the causal drug moxifloxacin. Had a course of treatment using the method of drug plasma leukocytapheresis, which includes the diagnosis, selection of chlamydia from different foci of infection of the patient, determination of their sensitivity to various etiotropic drugs, the sampling of venous blood from the patient, centrifuging the blood in the refrigeration unit, the section is a group of plasma, RBC mass and leukocyte suspension, replacement of plasma saline solution, the return of red blood cells in saline solution to the patient intravenously, activation of the leukocyte suspension He-Ne laser with λ=0.63 and radiation power of 10 MW, adding in a suspension with activated leukocytes moxifloxacin and intravenous drip of her patient. Conducted therapy resulted in mild syndrome common infectious intoxication, arthralgias syndrome, conjunctivitis, intensity and duration of urethritis and prostatitis, which is simultaneously accompanied by bacteriological readjustment of the organism from chlamydia. After the course of treatment is 10 days was achieved stable remission (observation period 1 year).
Example 2. Patient R., aged 35, has applied for medical assistance in the infectious diseases clinic complaining of severe pains in the perineum, smack in the testicles and suprapubic region, pain in the knee and ankle joints, painful rash on the glans penis. Urination was several casino. The elucidation of the history has allowed to establish that the patient had previously been treated for chronic prostatitis. A week before the development of the disease the patient was on holiday in Turkey. Sex denies there. Modern laboratory is priori methods revealed mixed chlamydial Ureaplasma-gardnerelly balanitis urinary infection, which type of prostatitis and vesiculitis with exonerative formations and microabscesses in the center and advanced replacement bubbles, the results of ultrasound scanning. Chlamydia has also been detected in conjunctivitis. The patient had a long, three or four courses of treatment with several drugs (antibacterial, immunomodulators, hepato - and angioprotectors) with physiotherapy and massages the prostate. Had a course of treatment (12 days) using the method of treating drug plasma leukocytapheresis. After treatment, the pain had stopped, the mucous membrane in the area of erosion at the head of the penis returning to a normal color came epithelialization. Bacteriological studies sosabravo materials from the urethra, conjunctiva, secret of the prostate gland, centrifugate urine and semen on sexually transmitted infections after treatment, after 1 month were negative.
A method for the treatment of urogenital chlamydial infection, including the determination of sensitivity of chlamydia isolated from the patient after setting the preliminary diagnosis, to various etiotropic drugs, characterized in that the patient take the blood, sequentially centrifuged and separated into plasma, red blood cell mass and leukocyte suspension, the ATEM remote blood plasma substitute saline solution, autoerythrocyte physiological solution is returned to the patient intravenously, and autolackiererei suspension activate the laser radiation from a He-Ne laser with λ=0.63 µm, then it add causal drug moxifloxacin and injected intravenously to the patient, repeating the treatment every day for 10-12 days.
FIELD: veterinary medicine.
SUBSTANCE: vaccine comprises as antigens cell suspension of pure cultures of a streptococcosus pathogen Streptococcus pneumoniae and an enterococcal pathogen Streptococcus fecalis isolated from suspension and prepared from damages organs of dead nutrias from the local epizootic focus wherein these cultures are grown separately in beef-extract broth with glucose with the concentration of microbial cells 4-5 billions in 1 cm3 and mixed in equal ratio. Also, vaccine comprises formalin and aluminum hydroxide in the following ratio of components, wt.-%: cell suspension of pure culture of streptococcosus pathogen Streptococcus pneumoniae isolated from a local epizootic focus in the nutrient medium with a titer 4-5 microbial cells in 1 cm3, 38.0-41.5; cell suspension of pure culture of enterococcal infection pathogen Streptococcus fecalis isolated from a local epizootic focus in the nutrient medium with a titer 4-5 billions of microbial cells in 1 cm3, 38.0-41.5; glucose, 1.0-2.0; formalin, 1.5-2.0, and aluminum hydroxide, the balance. Vaccine is harmful and possesses high specificity and effectiveness.
EFFECT: valuable properties of vaccine.
1 tbl, 5 ex
FIELD: veterinary science.
SUBSTANCE: on finishing the lactation one should inject once antimicrobial preparation "Difumast" per 5 ml into all udder lobes and, additionally, intramuscularly one should inject immunomodulating "preparation "Dostim" at the dosage per 25 ml for the first time, and in 40-45 d - the second. The innovation enables to shorten the quantity of "difumast. The innovation enables to shorten the quantity of "Difumas" injected.
EFFECT: higher accuracy of prophylaxis.
1 tbl;, 1 ex
FIELD: medicine, oncology, biochemistry, antibiotics.
SUBSTANCE: invention relates to variants used in treatment of cancer. Method involves administration in a patient needing with such treatment of the effective dose of 9-amino-6-deoxy-5-hydroxytetracycline or its salt, or 9-nitro-6-deoxy-5-hydroxytetracycline or its salt for aims in treatment of breast cancer, melanoma, myeloma and prostate gland cancer. Invention provides anti-tumor, anti-metastatic effect and the elevating viability index based on inhibition of matrix-destructing metalloproteinase by indicated derivatives of tetracycline and without significant body weight loss.
EFFECT: valuable medicinal properties of derivatives, enhanced effectiveness of treatment.
4 cl, 5 tbl
FIELD: organic chemistry, antibacterial agents.
SUBSTANCE: invention relates to an agent used against acid-resistant microorganisms containing derivative of pyridone carboxylic acid as an active component, its pharmaceutically acceptable salt or its hydrate that elicits high antibacterial activity against Mycobacterium tuberculosis and atypical acid-resistant microorganisms. Invention describes agent used against acid-resistant microorganisms containing compound represented by the following formula (I) its salt or its hydrate as an active component wherein R1 represents cyclic alkyl group comprising 3-6 carbon atoms that can comprise substitute(s) chosen from halogen atom; R2 represents hydrogen atom; R3 represents hydrogen atom; A1 represents incomplete structure represented by the formula (2): wherein X2 represents halogen atom, alkyl group comprising 1-6 carbon atoms or alkoxy-group comprising 1-6 carbon atoms; A1, A2 and A3 form incomplete structure of the formula: in common with carbon atoms combined with them; X1 represents halogen atom; Y represents hydrogen atom; Z represents phenylpiperazine substitute. Invention provides synthesis of pyridone carboxylic acid eliciting high antibacterial activity against Mycobacterium tuberculosis and atypical acid-resistant microorganisms in combination with good pharmacokinetics indices and safety.
EFFECT: valuable biological property of agent.
10 cl, 9 tbl, 10 ex
FIELD: medicine, microbiology, clinical practice.
SUBSTANCE: claimed method includes determination of pectin antioxidant activity (AOA) by chemiluminescent method and on the base of AOA pectin bactericidal properties are evaluated, wherein increased AOA indicates increased bactericidal properties. Method of present invention makes it possible to decrease time of pectin antibacterial property determination from 3 days to 30 min.
EFFECT: method of decreased labor intensity and cost.
1 tbl, 1 dwg
FIELD: organic chemistry, chemical technology, medicine, pharmacy.
SUBSTANCE: invention relates to a synthetic quinolone agent that is effective as medicinal agents, veterinary preparations, drugs used in fishing industry or as antibacterial preserving agents. Invention describes compound represented by the following general formula (I): as its separate isomers or their mixture, its salt and their hydrates wherein R1 represents cyclic alkyl group comprising 3-6 carbon atoms that can comprise a substitute chosen from halogen atom; R2 represents hydrogen atom; R3 represents hydrogen atom; R4 represents hydrogen atom, amino-group, hydroxyl group; A represents nitrogen atom or part of structure as given in the invention claim; each R5 and R6 represents independently alkyl group comprising 1-6 carbon atoms or hydrogen atom; n means a whole number 1 or 2. Also, invention describes antibacterial agent and therapeutic agent based on compounds of the formula (I) used in treatment of infectious disease, a method for preparing antibacterial agent, a method for preparing a medicinal agent used in treatment of infectious disease and using compound of the formula (I) for preparing an antibacterial agent and using compound of the formula (I) for preparing a medicinal agent used in treatment of infectious disease. Invention provides novel compounds possessing useful biological properties.
EFFECT: improved preparing method of agents, valuable medicinal properties of compounds and agents.
35 cl, 2 tbl, 15 ex
FIELD: chemistry of heterocyclic compounds, antibacterial agents.
SUBSTANCE: invention relates to agent used against acid-resistant microorganisms. Invention describes agent against acid-resistant microorganisms containing as an active component the compound represented by the general formula (1) or its hydrate wherein R2 represents hydrogen atom; R3 represents hydrogen atom; A1, A2 and A3 form incomplete structure of the formula: wherein A1 represent incomplete structure of the formula: wherein X2 and above described R1 can be combined to form six-membered cyclic structure comprising part of the parent nucleus wherein formed ring can comprise oxygen atom and, except for, can comprise alkyl group having 1-6 carbon atoms as a substitute; X1 represents halogen atom, hydrogen atom or amino-group; Y represents hydrogen atom; Z represents incomplete structure of the formula: . Agent elicits high antibacterial activity of broad spectrum and possesses good pharmacokinetics and safety also.
EFFECT: improved and valuable properties of agent.
3 cl, 9 tbl, 10 ex
FIELD: veterinary, vaccines.
SUBSTANCE: claimed vaccine contains as antigens pure culture cell suspension of causal organisms of salmonellosus Salmonella typhimurium and enterococcus infection Streptococcus fecalis, obtained by sampling of damaged organs of fallen nutrias from local epizootic focus, suspension preparation, seeding on differential diagnostic media, isolation of causal organism pure cultures. Culturing is carried out separately in meat-and-peptone broth with glucose at microbial cell concentration of 4-5 bln per 1 cm3, cells are mixed in ratio of 1:2, formalin and aluminum hydroxide are introduced in the next component ratio (mass %): pure culture cell suspension of causal organisms of salmonellosus Salmonella typhimurium isolated from local epizootic focus in broth with titer of 4-5 bln per 1 cm3 22.5-27.5; pure culture cell suspension of causal organisms of enterococcus infection Streptococcus fecalis isolated from local epizootic focus in broth with titer of 4-5 bln per 1 cm3 50.5-55.0-27.5; glucose 2.0-1.0; formalin 2.0-1.5; and balance: aluminum hydroxide.
EFFECT: associated vaccine with increased specificity and effectiveness without negative side effects.
1 tbl, 5 ex
FIELD: biotechnology, medicine, veterinary, in particular inhibition of vegetative and spore cell (Bacillus anthracis) vitality and malignant anthrax prophylaxis.
SUBSTANCE: invention relates to agent of bactericide action in relates to vegetative spore cells Bacillus anthracis. Agent contains bacteriolytic complex produced by Lysobacter sp.XL1. In method for malignant anthrax prophylaxis bacteriolytic complex produced by abovementioned bacterium strain is used as active ingredient. Said method includes subdermal injection in one dose not later than 3 hours after contamination, or application of dressing (drape) impregnated with active ingredient on place of possible exciter penetration, or treatment of possible contamination focus with aerosol.
EFFECT: bactericide agent without side effects and decreased prophylaxis time.
6 cl, 8 ex, 8 tbl
FIELD: cattle breeding; methods of the fodder production.
SUBSTANCE: the invention is pertaining to microballs containing the derivative of pleuromutiline and the method of their production and also to production of the fodder granules containing the microballs and to the method of their production. The microballs have the average size from 1 micron up to 5000 microns and represent the polymeric matrix particles, where the polymeric compound is selected from the shellac, the polymeric compound on the basis of cellulose, the acrylic acid or the methacrylic acid, the maleic anhydride, polyvinyl pyrrolidone or polyvinyl alcohol, and which is highly dispersed in the solid or liquid form with the derivative of pleuromutiline of formula 1 or its physiologically tolerable acid-additive salts and quarternary ammonium salts: , where R1 means vinyl, between 1 and 2 carbonic atoms there is a single bond, and Ra and Rb mean each separately from each other hydrogenen or halogen, and Т represents -CH2-S-(CH2)K-N(R5)(R6), where "k" means the integer from 2 up to 5; R5 andR6 mean each separately from each other C1-C6-alkyl or Т represents -CH2-S-C(CH3)2-CH2-NH-C(O)-CH(NH2)-CH(CH3)2. The method of preparation of the microballs includes production the polymer solution selected from the shellac, the polymetric compound on the basis of cellulose, the acrylic acid or methacrylic acid, the maleic anhydride, polyvinyl pyrrolidone or polyvinyl alcohol, by dissolution of the shellac, the polymetric compound in the organic solvent, having the low affinity to the paraffinic oil or the organosilicic oil and with the dielectric constant from 10 up to 40, with addition of the water. Then the produced solution is introduced at intermixing with the derivative of the pleuromutiline and receive the first organic phase, which is not admixed with the paraffinic oil or the organosilicic oil. The first organic phase introduce at the intense intermixing into the second phase consisting of the paraffinic oil or the organosilicic oil, and prolong intermixing of the produced mixture until the microballs are formed at evaporation or the solvent removal. The microballs are separated, flushed and dried. The fodder granules for the livestock includes the effective number of the indicated above microballs, the milled dry fodder on the vegetable or animal base with or without the additives, such as proteins, vitamins and minerals. The method of preparation of the fodder granules includes the careful intermixing of the microballs with the organic, ground and homogenized components of the fodder. Then they add the water or the steam in the mixture in the amount from 5 up to 10 % of the total mass, press the mixture in the fodder rods at the heightened temperatures from 60 up to 80°С, and divide the rods into the fodder granules. The invention ensures stabilization of the pleuromutiline, which stays undamaged at the granules preparation and is kept safe for the rather long duration of storage.
EFFECT: the invention ensures stabilization of the pleuromutiline, which stays undamaged at the granules preparation and is kept safe for rather long duration of storage.
6 cl, 3 tbl, 3 ex
SUBSTANCE: method involves administering intraosseous Milgamma solution injection into periodontium tissues. Introduction of on the left and right side are alternated within mandible and maxilla innervation zone. 2-3 injections are introduced into the lesion focus. Electro-odontodiagnostic procedure is carried out before and after drug introduction. The number of injections is determined upon electro-odontodiagnostic procedure data and periodontium tissue injury severity degree. Gel form Milgamma applications are done on alveolar process. Semiconductor laser radiation treatment is applied with power of 5-6 mW in pulsating mode, with frequency of 1.5-4.0 kHz and 2-3 min long exposure. The number of procedures per treatment course is equal to 5-8 depending on periodontium tissue injury severity degree.
EFFECT: enhanced effectiveness of treatment; increased membrane permeability; improved microcirculation conditions.
FIELD: medicine, gastroenterology.
SUBSTANCE: the present innovation deals with carrying out complex medicinal therapy and laser therapy simultaneously. Laser therapy should be prescribed differentially. At disease duration being under 7 yr and light degree of disease flow one should carry out daily laseroreflexotherapy due to contact technique. 10 procedures/course. Wave length being 1.3 mcm, radiation power 1 mW, modulation frequency 2.4 Hz. Points V19, F14, VC12, VB24 should be affected daily. The impact upon the points G14, E36, E25 should be symmetrically alternated every other day at affecting the points MC6 and RP6 symmetrically. At disease duration being above 7 yr and either average or severe degree of disease flow it is necessary to fulfill intravenous laser blood irradiation at wave length being 0.63 mcm, radiation power at a light guide's edge being 1.5-2.0 mW, the duration of the procedure - 20 min, therapy course - 7 procedures. The innovation shortens terms of therapy, widens the chance for applying noninvasive methods and increases the efficiency of medicinal therapy conducted.
EFFECT: higher efficiency of therapy.
2 ex, 6 tbl
SUBSTANCE: method involves exposing anterior eye segment with low intensity infrared laser radiation operating in continuous mode. Wavelength is equal to 0.95 mcm, radiation power density from 0.2 to 0.45 mW and treatment session duration equal to 3-5 min. The procedure is given every day with total treatment course being 3-5 procedure long.
EFFECT: enhanced effectiveness in removing reactive trabecular tissue edema; reduced protein viscosity in anterior chamber fluid and prostaglandins and vasoactive mediators release; enhanced effectiveness in treating moderate and severe myopia patient having glaucoma and peripheral choroidoretinal dystrophy.
SUBSTANCE: method involves exposing blood to pulsating intravenous laser radiation of 2 W power. Pulse duration is 260 ns, pulse succession frequency is 10000Hz, wavelength is 0.89 mcm and action time is 15 min.
EFFECT: accelerated treatment course; excluded adverse side effects; reduced toxin concentration in blood.
SUBSTANCE: method involves introducing photosensitizer, recording level of its accumulation in tumors, carrying out photodynamic therapy. Pyrogenal is introduced at a dose of 40-1000 mkg/csm2 before introducing photosensitizer into the tumor base with tumor temperature being recorded until stable tumor tissue hyperemia being achieved for 12-24 h. Then, the tumor is smeared with composition containing 3-10 mg of photosensitizer, gel "Ultramix" and 20-200 mcg of Pyrogenal. Treatment with ultrasound is applied with intensity 0.2-0.4 W/cm2, in labile continuous mode within 5-8 min. The composition is left on skin for 1-2 h. Then, photodynamic therapy session is uniformly carried out over the whole tumor area, with total number of 3-5 procedures per treatment course.
EFFECT: enhanced effectiveness of treatment; prolonged optimum photosensitizer dose being supported in tumor, sufficient for effective photodynamic therapy action.
SUBSTANCE: method involves intravenously introducing chlorine row photosensitizer as liposomes at bolus dose of 1.0-1.5 mg/kg 2 weeks prior to transplantation. The whole cornea surface is exposed to laser radiation field-by-field with the neighboring fields overlapping over 5% of area in 10-15 min after photosensitizer introduction is over. The wavelength corresponds to photosensitizer liposome light radiation absorption maximum. Power density is equal to 100-120 J/cm2. Irradiation is carried out within circle in moving from periphery to the center.
EFFECT: emptied and regressed newly formed cornea blood vessels with no bleeding occurred in transplanting cornea.
SUBSTANCE: method involves puncturing and catheterizing general femoral vein or general carotid by applying Seldinger techniques. Introducer of 4-9F in diameter set. Conducting catheter is coaxially introduced through it into the injured cerebral bloodstream bed. Radiopaque flexible catheter of 3-6F in diameter with light guide is coaxially introduced through it to blood vessel occlusion place. Flexible quartz - quartz or quartz-polymer light guide diameter is equal to 100-200 mcm. Heparinized physiological salt solution is introduced during laser installation operation at a rate not less 0.1 ED of heparin in 1 ml of physiologic saline. Solution introduction rate is not less than 1 ml/s. Then, the conducting catheter brought down. The distance between light guide end face and irradiated surface is 1-5 mm long. High-energy laser of 20 W power is applied. Pulsating periodic pulsating mode is applied. Radiopaque substance is periodically introduced in small dozes and carry out during laser radiation treatment and X-ray TV control over passability and full-scale lumen restoration in the injured bloodstream bed zone.
EFFECT: enhanced effectiveness of treatment; provided ischemized brain tissue restoration and its subsequent regeneration.
SUBSTANCE: according to a certain technique one should puncture and catheterize a total femoral artery along with applying an introducer of 6-9 F diameter. Through this introducer one should coaxially apply and install the first conductor catheter of 5-8 F diameter and modeled distal part in the trunk of the inner carotid artery; through it is necessary to coaxially apply the second modeled conductor catheter of 4-5 F diameter to be superselectively installed in median cerebral and anterior cerebral arteries. Successively, through it one should coaxially apply a flexible roentgenocontrast catheter of 2-3.5 F diameter with a movably located flexible quartz-quartz or quartz-polymeric light guide at diameter of light-conducting fiber ranged 50-100 mcm and total diameter of about 300-400 mcm. The light guide should be moved along the vessel towards the site of microcirculatory vascular tract lesion in distal departments. The first conductor catheter should be descended and applied in descending part of aortic arch, the second one should be descended and applied in proximal part of total carotid artery. Then it is necessary to affect with low-energy laser, the power being 20 mW, the mode should be either uninterrupted, or impulse, or combined. During irradiation it is important to introduce heparinized physiological solution at 0.1 U heparin/ml physiological solution, the rate of injection corresponds to 0.1 ml/sec, not less. The innovation enables to reconstruct microcirculatory vascular tract in brain along with regeneration of surrounding cerebral tissues.
EFFECT: higher efficiency of therapy.
FIELD: medicine, oncology.
SUBSTANCE: the present innovation deals with treating disseminated forms of melanoma along with metastases into regional lymph nodes and intracutaneous metastases, the diameter being up to 2 cm at the depth of location up to 1 cm together with a locus. The method deals with intravenous injection of anti-tumor preparation as a photosensitizer (PS) of chlorine class into cubital vein of one hand at the dosage of about 1.5 - 2.0 mg/kg for 40-60 min and simultaneously, fulfill intravenous laser blood irradiation (IVLBI) into cubital vein of another hand at wave length corresponding to the maximal absorption of light radiation with PS, at the dosage of irradiation being 600-900 J/sq. cm. In about 3-3.5 h it is important to carry out spectral-fluorescent diagnostics (SFD) of metastatic foci and if the ratio of their fluorescence against that of surrounding tissue is not less than 3:1 one should conduct transcutaneous laser irradiation (TLI) of metastatic foci at wave length being correspondent to maximal absorption of light radiation with PS. In 24 h on finishing TLI one should carry out additional laser irradiation. The innovation provides either complete or partial regression of metastases along with considerable decrease of the possibility for dissemination out of metastatic foci.
EFFECT: higher efficiency of therapy.
3 cl, 1 ex
FIELD: medical engineering.
SUBSTANCE: device has optical block connected to processor having two radiators producing visible spectrum laser radiation and near infrared region in optical bandwidth, respectively. They are brought through converter socket to fiber-optical light guide having replaceable diffuser on distal end, a temperature sensor control, the power supply, programming port, data input block and display. The temperature sensor control is carried out by means of coaxially distributed thermodes connected to the processor and fixed on optical fibers end faces, movable in longitudinal direction in hollow needle tip canals inclined towards periphery.
EFFECT: enhanced effectiveness of treatment; exact tumor tissue localization, simultaneous endoscopic control and automatically adjustable irradiation process.
2 cl, 3 dwg
FIELD: medicine, narcology.
SUBSTANCE: method involves sampling venous blood in volume less 10-155% of total circulating blood for the first 24 h of disease and cellular mass of blood is separated from plasma. Mexidol in the dose 3 mg/kg of body mass is added to one portion and sibazon in the dose 0.25 mg/kg of body mass is added to another portion. Each portion is incubated separately and infused in a patient being mexidol-containing portion is infused firstly and then sibazon-containing portion is infused at temperature of a patient body. Method provides accelerating elimination of intoxication syndrome and reducing the treatment period based on the fixed therapy. Invention can be used in treatment of alcoholic delirium.
EFFECT: enhanced effectiveness of treatment.