Method of reducing nickel and lead levels in blood and milk of cows in a man-caused province

FIELD: veterinary.

SUBSTANCE: naturally occurring minerals suitable for use in enterosorption process, especially in sorption of nickel and lead, are proposed. Zeolite originated from Kamyshlovsk deposit situated in Sverdlovsk region is added to main dairy cow diet in amount 0.15-0.20 g per 1 kg animal weight a day over a 25-30 day period.

EFFECT: achieved environmentally safe produce.

4 tbl

 

The invention relates to veterinary medicine and can be used in man-made provinces, the environmental objects that are exposed to contamination by salts of heavy metals, and feed rations animals contain high levels of salts of Nickel and lead.

In veterinary practice is known and proposed a wide variety of ways enterosorption salts of Nickel and lead from the body of animals, which assume the use of the following tools: sorbent MAM-90, carbon white, microcrystalline cellulose (MCC), sodium thiosulfate, Anaconda-In, decoction of licorice root and Polisorb EAP.

Known methods of animal feeding sorbent MAM-90 in two periods of 10 days each, with an interval of 5 days at a dose of 20 g per head per day. Current top ferrocyanide-betontage sorbent MAM-90 is hexacyanoferrate (II), potassium iron (III), as well as bentonite and gelatin. This drug is obtained from the waste of the wine industry in the process of reducing the salt content of iron in wine and clarification of wine [4].

However, given the fact that the sorbent MAM-90 has a specific action detoxification of radionuclides, requires scientific justification for its use with the aim of excretion of salts of Nickel and lead.

The known method of eliminating heavy metals by the application of white soot. B is barking soot - tankodesantniki white powder with a mass fraction of silica is not less than 87%. Contains up to 6.5% moisture and impurities of oxides of aluminum, calcium, sodium, chlorides, fluorides and sulfates. The sorbent is injected in the form of supplements to the basic diet at the doses of 30 g per animal per day, or 60 mg/kg of body weight during the year.

Add white soot in the diet of cows reduces the concentration of nitrates in milk by 13%, zinc - by 14%, copper 5%of cadmium by 13%, lead by 13% and HOP - by 15%. While the average daily milk yield increased by 12% [7].

The disadvantage of this method, in our opinion, is the lack of clear patterns in the usage of the sorbent taking into account the morphological and physiological characteristics scar ruminant digestion.

Prolonged use of unnatural food accompanied by impairment of motor function of the rumen and, as a consequence, the development of hypotension.

The known method of removing lead from the body of animals by feeding microcrystalline cellulose (MCC). Currently developed new technology for the production of feed microcrystalline cellulose (MCC), which is obtained from all types and brands of cellulose by mechanochemical hydrolysis in solutions of inorganic acids. MCC able to rid the body of animals nitrogen compounds and lead.

According to research [1], MCC is obtained and the cotton fiber, is a chemically inert, does not contain chemical additives dry fine powdery material of white color, odorless, taste, in water forming a gel.

The introduction of a daily diet of piglets MCC at a dose of 700 mg/kg for 21 days was accompanied by the absence of clinical signs of lead intoxication in animals.

High adsorption capacity of the drug is related to its crystalline structure, and "purification" mechanically mucosa of the gastrointestinal tract.

However, when man-made provinces of the southern Urals in the feed ration of the animals taken in excess, there are not one, but several toxicants, the application of the MCC will be accompanied by a low therapeutic and pharmacological effect.

There is a method of feeding sodium thiosulfate at a dose of 25.0 g per head per day for 10 days. Transparent crystals of the drug is readily soluble in water and dissolve in the acidic environment of sulphur and sulphur dioxide.

Sulfur in the time of his selection acts on restorative proteins, preventing their transition to tighter joints. In addition, sodium thiosulfate acts regenerates the body of the animal, a positive effect on the redox processes, reduces acidosis, increases diuresis and thus sposobstvuet is the release of toxic products from the body.

In case of poisoning by heavy metals sodium thiosulfate acts as an antidote, turning them into less toxic compounds - non-toxic sulfides [2].

However, given the fact that sodium thiosulfate is preceded by blockade mainly sulfhydryl groups of proteins and the effect of certain toxins (mercury, arsenic, copper, lead) and to a lesser extent before the blockade carboxyl groups of amino acids in man-made provinces of the southern Urals its use is accompanied by insufficient detoxification effect.

There is a method of correction of heavy metals in animals by oral administration of Anaconda-In, in the form of a 10% solution at a dose of 0.5 mg/kg 1 time per day for 3 days. After 5 days of break Erakond-appoint the same scheme [6]. Currently in veterinary medicine studied therapeutic effect of Anaconda (extract condensed vegetable) is a biologically active drug, derived from alfalfa. The product contains up to 20 amino acids, a wide range of trace elements, organic acids, carbohydrates, vitamins, secondary metabolites of plants, in particular, a class of phenolic compounds. Testing of the drug on animals and the bird showed its anti-inflammatory, choleretic, hepatoprotective, antibacterial, antiviral properties, high immunological activity.

p> Erakond-In:

1) along with a natural complex of micronutrients supplemented by a set of elements, performing, first, the role of antagonists in relation to a number of metals, and secondly, is able to restore the lack of trace elements in feed;

2) contains flavonoids, are capable of forming stable complexes with heavy metals, and similar chemically, coumarins;

3) is a concentrate of vitamins, including ascorbic acid and Tocopherols (natural antioxidant).

Erakond-In contributes to the formation of complexes with heavy metals, while complexing antigen can be charged particles of the drug. The disadvantage of this method of correction is the fact that Erakond-In is composed of a large number of trace elements, which after its use in the animal body is able to show antagonism and display essential elements necessary for life.

The known method of removing toxic elements from the body of animals by daily watering with potable water 470-570 ml of decoction of licorice root (1:20) for 45 days [3]. This method is quite effective, as the decoction of licorice root helps enveloping salts of heavy metals and prevents their absorption in the gastrointestinal tract.

However, the disadvantage of this method is difficult technological process, as well as contamination with heavy metals is exposed to all livestock animals and in this regard to conduct detoxification activities require large amounts of broth. In addition, there is no confidence that the assembled and prepared on-site ecological risk licorice root will not contain high levels of toxic elements.

The closest analogue is the way the correction of the excessive content of toxic elements in the body of animals by introducing Polisorb EAP (veterinary oral) 150 mg/kg 2 times in 2 days with 5-day intervals [5].

Polisorb EAP - highly dispersed pyrogenic silica, procainamidesee properties which provide the binding and excretion of Exo - and endo-toxins, including heavy metals. The method is quite effective because Polisorb EAP is able to bind not one, but several toxicants. However, a significant drawback of the mentioned method is the low adaptability of the process, due to the fact that the dosage of the drug is carried out per kilogram of body weight, and the use of large amounts of the drug contributes to the structuring of water in the rumen of ruminants, and this phenomenon is accompanied by a decrease scar PI is awarene.

The aim of the invention is the finding cheap new technological ways of elimination of salts of Nickel and lead from the body of the lactating cows induced province.

This goal is achieved by the fact that dairy cows in ecologically unfavorable areas in the composition of the feed ration mixed with concentrates enter the zeolite kamyshlovskogo deposits at a rate of 0.15-0.20 g/kg body weight once a day for 25-30 days.

Comparative analysis of the proposed solutions with the prototype shows that the inventive method differs from the known fact that for pharmacokineti use natural mineral zeolite kamyshlovskogo field of Sverdlovsk region.

Conducted at the Institute of Mineralogy of Sciences x-ray fluorescence analysis of the studied mineral showed that it consists of the following chemical elements, %: K2On - 3,19; Na2O - 1,77; CaO - 2,87; MgO - 0,88; Al2About3- 13,6; SiO2- 78,4; Fe2About3- 1,84; TiO2- 0,34; SO3- 1.4 and other trace elements.

The chemical composition of zeolites and zeolite minerals used in diets of farm animals and poultry, described Segmentum (1994).

Zeolite kamyshlovskogo field differs from other zeolites of the fields developed on the territory of the Russian Federation, a high content of oxide is silicon and aluminum, which gives the mineral pronounced porosity and higher sorption properties.

Thus, the claimed method meets the criteria of the invention of "Novelty."

The analysis of the prototype and other methods in the field of veterinary medicine did not reveal any sign, similar to the claimed solution, which allows to make a conclusion on compliance of the way the criterion of "substantial differences".

The method is implemented as follows. Zeolite kamyshlovskogo field in a dose of 0.15-0.20 g/kg body weight once a day for 25-30 days introduced into the diet of dairy cows.

Natural zeolites are microporous framework silicates crystal structure containing channels and cavities occupied by large ions and water molecules. They have considerable freedom of movement, which leads to ion exchange and reversible dehydration. The primary building unit of zeolite framework is the tetrahedron, the center of which is occupied by an atom of silicon or aluminum, and the tops are four atoms of oxygen. Each oxygen atom is shared by two tetrahedra. Their combination forms a continuous frame. Thanks to strictly reserved to the pore size of the inner cavities of natural zeolites possess a molecular-sieve properties are good adsorbents for many inorganic and organic substances, including h the following and salts of heavy metals.

Given the above properties, we decided to use the zeolite kamyshlovskogo field as enterosorbent that reduce the levels of Nickel and lead in the body of animals.

Preliminary experimental studies have tested different doses of mineral 0,1; 0,15; 0,20; 0,25; 0,30; 0,35; 0,40; 0,45; 0,5 g/kg body weight and the duration of its application. It was found that to achieve a pronounced pharmacological effect of the removal of Nickel and lead from the body of the cow must be used in animal diets mixed with concentrates zeolite kamyshlovskogo deposits at a rate of 0.15-0.20 g/kg body weight once a day for 25-30 days. In addition, given the significant anatomical and morphological and physiological peculiarities of multi-chambered stomach of ruminants, have studied the influence of different doses of zeolite kamyshlovskogo field on the performance of the rumen contents, as it cleaved a significant portion of food. Research and production experience in the study of this issue was conducted on the farm "Drobysheva" Trinity district. The objects of the external environment of the economy is exposed to contamination by emissions Troitskaya GRES, which works on brown Ekibastuz coal of high ash content. Priority pollutants in this household are Nickel and lead free. Thus, the research was not primarily the Leno, introduction in addition to the basic diet of zeolite kamyshlovskogo field in a dose of 0.15-0.20 g/kg body weight once a day helps to normalize indicators scar content, especially evident on the 30th day of research. Thus, the levels of the total number of AGV's (volatile fatty acids) was 17.8% higher than in the control animals. In addition there was a significant increase in the percentage of acetic and propionic acids, with a total increase in the number of infusoria.

Thus, studies strongly suggest that the recommended dose of zeolite kamyshlovskogo field and the multiplicity of its application contributes to stimulation of indicators scar digestion.

For the implementation of the proposed method experimental study was conducted on the basis of the SEC "Ostroleka" Nagaybaksky district of Chelyabinsk region. Land use SEC "Ostroleka" according to the regional exploration is located in the zone of surface deposits of Nickel ore, and the economy is in the area of industrial emissions of Magnitogorsk metallurgical combine. In this connection, the main pollutants of interest in the external environment economy are Nickel and lead free.

Monitoring the trophic chain management showed that soil samples taken from various on the it sector, contain Nickel 20.1-55,4% more MAC.

The content of the accompanying element of lead in soil samples also exceed the MPC 14.3-31.6% respectively. High levels of toxicants have been identified in food crops farming (hay, haylage, straw, concentrates).

In the experiment to study the effect of zeolite kamyshlovskogo field of the Sverdlovsk region, as enterosorbent salts of Nickel and lead was included 24 head of milk cows at 3-4 months of lactation, the average productivity of 8.2-8.5 kg of milk. Cow Simmental breed at the age of 3.5 to 4 years to live weight 480-520 kg

Cows were kept in the model barn 200 head, milking, feeding and care of all animals were equal.

According to the principle of analogues of the experimental animals were divided into 3 groups. The first group served as control, it was judged on the content of Nickel and lead in blood and milk, and excretion of toxicants with urine and feces. Cows in the second group once a day for 30 days in the feeding ration mixed with concentrates was introduced zeolite kamyshlovskogo deposits at a rate of 0.15 g/kg body weight.

Cows of the third group were injected Polisorb EAP, used in the method for correcting the levels of Nickel and lead, which was used as a prototype declared.

In accordance with the requirements of method Polisorb the EAP was introduced from the calc is and 150 mg/kg of body weight 2 times a day 2 day and 5-day interval. Blood and milk from experimental and control groups were tested Nickel and lead on the 7th; 14th; 30th day of the experiment and compared with values obtained before the experiment.

The results obtained are presented in table 1 and 2.

Table 1

Dynamics of the level of Nickel and lead, mg/l in the blood of experimental animals (M±m; n=8)
GroupNormaTo experienceDay research
71430
1 ControlNickel
0,122,05±0,042,03±0,112,07±0,062,04±0,09
2 the claimed method0,122,03±0,061,17±0,140,19±0,050,15±0,02
3 prototype0,121,99±0,031,48±0,121,21±0,080,43±0,07
Lead
1 control0,250,41±0,020,39±0,160,40±0,120,39±0,09
2 the claimed method 0,250,40±0,070,37±0,060,29±0,050,26±0,04
3 prototype0,250,39±0,090,38±0,020,32±0,020,30±0,13

Analyzing the data of table 1, it should be noted that the high content in the feed ration is Nickel and lead accompanied by a significantly high concentration in the blood. So the animals of the control group, the levels of Nickel prior to the beginning of the experiment in 19-20 times higher than the physiological norm. During experimental studies its contents were not changed and were above the permissible limits.

Introduction in the diet of zeolite kamyshlovskogo field experimental group of cows was accompanied by the reduction of Nickel, from 7-x, but is especially significant decrease was detected on the 30th day. On the 30th day the level of toxicant decreased by 92,65% compared with the control group. In the third experimental group of cows, which used Polisorb EP, was a natural reduction of Nickel in all periods of the studies, but the most significant on the 30th day. Thus, the content of Nickel declined 79,0%, but exceeded physiologically acceptable norm in 4 times.

Similar results were obtained for lead levels in the background of the introduction in the diet of the zeolite To mislavskogo field.

In the blood of cows in the control group the level of lead content in the course of the experiment was higher than normal.

The application of zeolite kamyshlovskogo field was decreasing lead levels especially on the 30th day of research. Thus, the level of lead in this period declined by 33.4%.

In the third group of experimental animals under the influence of Polisorb EP on the 30th day of research lead levels decreased by 23.1% and was higher than normal.

High levels of Nickel and lead in the body of dairy cows results in increased excretion with milk.

Experimental materials are presented in table 2.

As shown by the data in table 2, the milk of the cows of the control group during the experimental period has a high concentration of Nickel level, which is 10 times higher than the MPL.

Table 2

The dynamics of the levels of Nickel and lead in the milk of laboratory animals (mg/l); M±m; n=8)
GroupMPC (PREV. doPost. con.)To experienceDay research
71430
group 1 control0,1Nickel
1,14±0,071,12±0,041,15±0,111,13±0,03
group 2 (the claimed method)0,11,13±0,060,87±0,070,46±0,10,12±0,08
group 3 (prototype)0,11,15±0,030,98±0,170,66±0,050,38±0,13
group 1 control0,1Lead
0,20±0,10,22±0,090,24±0,120,21±0,02
group 2 (the claimed method)0,10,21±0,130,17±0,060,14±0,090,11±0,04
group 3 (prototype)0,10,20±0,090,18±0,020,16±0,040,14±0,05

In the third experimental group under the influence of Polisorb EAP was a natural reduction of Nickel in all periods of the studies. So, on the 7th day level fell by 12.5%. On the 14th - 52,7%, 30th by 66.4%. However, it should be noted that the level of Nickel in blood by the end of the experimental period was higher than the MPL by 280%.

In the second experimental group after application of zeolite kamyshlovskogo field detoxification eff the CT was expressed much better.

So on the 7th day, the level of Nickel decreased by 22.4%, on the 14th of 60%, on the 30th by 89.4%.

The lead levels in the milk of cows of the control group throughout the experiment was significantly higher than the MPL.

In the third experimental group under the influence of Polisorb EAP has revealed reduction of lead content especially on the 30th day. Thus, the level of lead in this period declined by 33.4%. However, it should be noted that the level was 30% higher than the MPL.

The use of zeolite kamyshlovskogo deposits contributed to a more rapid and pronounced reduction of lead in milk of cows of the second experimental group. So, by the end of the experiment, the level of lead decreased by 47.7% and corresponded to the MAC.

It is known that the main ways to remove toxins from the animal body are the kidneys and the gastrointestinal tract. In this regard, experimental studies have analyzed the feces and urine of laboratory animals, which are presented in table 3 and 4.

Table 3

Dynamics of excretion of Nickel and lead from the body of experimental animals faeces (mg/kg; M±m; n=8).
GroupTo experienceDay research
71430
group 1 (control) 7,9±0,14Nickel
8,3±0,198,5±0,178,2±0,13
group 2 (the claimed method)8,1±0,1612,3±0,0919,1±0,2327,9±0,11
group 3 (prototype)8,2±0,219,6±0,1411,7±0,1614,6±0,15
group 1 (control)6,8±0,11Lead
7,1±0,167,4±0,247,0±0,51
group 2 (the claimed method)7,1±0,499,6±0,1316,2±0,3723,4±0,44
group 3 (prototype)7,3±0,228,16±0,3511,8±0,3314,1±0,5

The analysis of table 3 shows that under the action of applied chelators increase of toxins in the feces of the animals of the third and especially the second experimental group. This phenomenon suggests that the process of adsorption of toxins carried in the gastrointestinal tract and prevents their absorption into the blood and lymph. The use of Polisorb EAP in the third experimental group was increased excretion of Nickel in the feces 78,0%. Zeal the t kamyshlovskogo deposits contributed to a more substantial removal of Nickel from the body. So, on the 30th day of studies, the level of Nickel in the feces of animals was 240% higher than in the feces of the animals of the control group.

In addition, the use of chelators helped lead elimination from the organism of experimental animals.

So, on a background of application of Polisorb EAP level of lead in the feces of animals increased by 10%, and under the influence of zeolite kamyshlovskogo fields of 234.2%, respectively, indicating a more efficient process of adsorption of toxicant.

Table 4

Dynamics of excretion of Nickel and lead from the body of experimental animals with urine (mg/l); M±m; n=8)
GroupTo experienceDay research
71430
group 1 (control)22,6±0,16Nickel
23,7±0,4924,9±0,1725,6±0,37
group 2 (the claimed method)24,7±0,3326,5±0,1728,4±0,1629,9±0,32
group 3 (prototype)25,4±0,1927,7±0,1329,1±0,1126,3±0,12
group 1 (control of the) 16,4±0,53Lead
17,3±0,1218,2±0,1419,4±0,44
group 2 (the claimed method)15,8±0,4316,2±0,3017,8±0,4120,6±0,50
group 3 (prototype)17,6±0,2218,3±0,3119,8±0,4820,7±0,38

Analyzing the elimination of Nickel and lead-free urine from the body of experimental animals it should be noted that significant differences under the influence of chelators have been identified.

Thus, the main route of excretion of toxicants on the background of the use of sorbents is the gastrointestinal tract, thereby reducing levels of Nickel and lead in blood, respectively, and in the milk of cows induced province of the southern Urals.

Summarizing the obtained results, we can conclude that the advantages of the inventive method compared to the prototype and the other suggestions in this area allow us to recommend the zeolite kamyshlovskogo field for use in veterinary practice in areas with a high content of Nickel and lead in the food chain, along with the blood and milk of dairy cows at a rate of 0.15-0.20 g/kg once a day for 25-30 days to reduce visiva the Oia toxicants in the blood and excretion of their milk.

The list of references

1. Argunov M.N., Safronov L.V., noses, E.E., Zhukov, IV drug Use MCC as adsorbent intoxication of animals with lead // Actual problems of veterinary surgery. - Voronezh, 1999. - S-176.

2. Kotov N.A. Farmacocinetica abnormal levels of heavy metals in the food chain technogenic zones, Miass: author. Diss. on saisc. academic step. Kida. wet. Sciences. Troitsk, 2001, to 23 C.

3. Kuznetsov YEAR, Use of natural zeolites in animal breeding: a Review. inform. - M., 1994, - 44 C.

4. Lukasova I.A., Rabinovich M.I. Kinetics of heavy metals in animals on a background of application of licorice root. J. Veterinary Medicine. - 1999, No. 3, - p.45-47.

5. Sevastyanova N.A. Veterinary Toxicological assessment of heavy metals in the area of the coal mine "Korkino" JSC Uralcement and correction of their excretion of cattle: author. Diss. on saisc. academic step. Kida. wet. Sciences. Troitsk, 2001, to 23 C.

6. Tairov, A.R. efficacy of the Polisorb EAP metabolic disorders in the body of cattle / materials international. scientific-practical. Conf., ]. 70th anniversary UGUM "Actual problems of veterinary medicine, livestock, commodities, social studies and training in the southern Urals", Troitsk, 1999, - s-118.

7. Tairov, A.R., Elimination of swoystwa.preparat Erakond - C. J. Veterinary physician, Kazan 2001 №2(6), S. 50-52.

8. Shaposhnikov A.N., Gabruk N.G. Biochemical value of milk when used in the diet of the cows feed sorbents. The Intern. scientific-practical. conference. "Ecol.-genetic. the problem alive-VA and Ecol. safety. Technology of food production" - dobrovici, 1998, - p.á192-193.

The method of reducing levels of Nickel and lead in blood and milk of cows induced province, which includes the introduction in the diet of animals mineral that has adsorption properties, characterized in that the adsorbent Nickel and lead used zeolite kamyshlovskogo field Sverdlovsk region at a rate of 0.15-0.20 g/kg body weight once a day for 25-30 days.



 

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1 tbl

FIELD: animal science.

SUBSTANCE: it is necessary to introduce perorally an alcohol-honey extract of walnuts of milk-wax maturity at adding glycine and flower pollen. The preparation should be introduced 5 d before and after the impact of stress factors 30 min before feeding per 10-15 ml/animal daily. As a result of applying the suggested preparation the body weight losses in animals are decreased considerably, the body weight gain is increased along with the quantity and quality of animal production.

EFFECT: higher efficiency of prophylaxis and correction.

1 ex, 2 tbl

FIELD: veterinary science.

SUBSTANCE: the innovation deals with supplementing the diet with iodinated common salt and a medicinal preparation perorally as metronidasol and a preparation of sulfanyl amide group. Additionally, one should introduce a diuretic, and as medicinal preparation one should additionally use penicillin sodium salt intramuscularly and 0.5%-metronidasol solution intravenously. Moreover, for pregnant cows of dairy herd the preparations should be introduced once annually for the whole productive period in the following sequence: a 6-d-long course up to calving dealing with introducing metronidasol at the dosage of 1.5 g/animal and norsulfasol or sulfadimezine at the dosage of 1 g/animal. At calving day it is necessary to inject intravenously 0.5%-metronidasol solution at the dosage of 200 ml/animal thrice in 8 h. Since the 2nd to the 5th d inclusively it is important to introduce metronidasol perorally at the dosage of 2.5 g/animal and norsulfasol or sulfadimezine at the dosage of 1 g/animal. Since the 6th to the 15th d inclusively one should inject intramuscularly penicillin sodium salt at the dosage of 2 mln U/animal - twice daily. On the 16th d one should inject intravenously 0.5%-metronidasol solution at the dosage per 200 ml/animal thrice in 8 h. With prophylactic purpose for stud bulls it is necessary to introduce preparations twice annually at 6-mo-long intervals according to the following scheme: during the 1st and the 2nd d - intravenously 0.5%-metronidasol solution at the dosage of 300 ml/animal thrice at 8-10-h-long interval. Since the 3d to the 5th d inclusively - perorally metronidasol at the dosage of 2.5 g/animal and norsulfasol or streptocid at the dosage of 2 g/animal. Since the 6th to the 15th d - intramuscularly penicillin sodium salt at the dosage per 3 mln U/animal twice daily. On the 16th d - intravenously 0.5%-metronidasol solution at the dosage of 300 ml/animal thrice in 8 h. With prophylactic purpose for cattle youngsters the preparations should be introduced starting since 1-mo age according to the following scheme: during the 1st d - intravenously 0.5%-metronidasol solution at the dosage of 10 ml/animal thrice at 8-10-h-long interval. During ten days - perorally metronidasol at the dosage of 0.125 g/animal and norsulfasol at the dosage of 0.5 g/animal once daily. On the 13th and the 14th d - intravenously 0.5%-metronidasol solution at the dosage of 10 ml/animal thrice at 8-10-h-long interval, for 4-mo-aged calves during the first 2 days - intravenously 0.5%-metronidasol solution at the dosage of 20 ml/animal thrice at 8-10-h-long interval. During 18 days - perorally metronidasol at the dosage of 0.5g/animal and norsulfasol at the dosage of 0.5g/animal once daily, for 6-mo-aged calves during 10 days running - intramuscularly penicillin sodium salt at the dosage of 500 thousand U/animal twice daily. For 12-mo-aged calves during the first 2 days - intravenously 0.5%-metronidasol solution at the dosage of 100 ml/animal thrice at 8-10-h-long interval, since the 3d to the 5th d inclusively - perorally metronidasol at the dosage of 1 g/animal and norsulfasol or streptocid at the dosage of 1 g/animal once daily, since the 6th to the 12th d inclusively - intramuscularly penicillin sodium salt at the dosage of 1 mln U/animal twice daily. For 17-mo-aged calves during the first 2 days - intravenously 0.5%-metronidasol solution at the dosage of 150 ml/animal thrice at 8-10-h-long interval; since the 3d to the 5th d - perorally metronidasol at the dosage of 1.5 g/animal and norsulfasol at the dosage of 1 g/animal; for 18-mo-aged calves before pairing for 10 d - intramuscularly penicillin sodium salt at the dosage of 1 mln U/animal twice daily. The innovation is of high efficiency and enables to shorten the terms of therapy along with widened groups of animals under treatment and increased action of medicinal preparations.

EFFECT: higher efficiency.

3 cl

FIELD: animal farming.

SUBSTANCE: method involves using colamine as means for reducing losses of young cattle products during transportation and pre-slaughtering keeping of young cattle; feeding colamine to animals 7 days before transportation in an amount of 20-50 mg/kg of live weight.

EFFECT: reduced losses of animals live weight and increased resistance to stress effects.

5 tbl, 1 ex

FIELD: animal science.

SUBSTANCE: it is necessary to apply mitesel and glycosel additives as antidepressants at feeding animals 5 d before their transportation at the rate of 30 mg/kg body weight. The innovation enables to decrease negative impact of transportation stress and reduce the losses of body weight in cattle youngsters.

EFFECT: higher efficiency of prophylaxis.

1 tbl

FIELD: veterinary medicine.

SUBSTANCE: method involves taking animal blood samples, producing erythrocyte suspension by mixing blood and physiologic salt solution and centrifuging it. Reaction is carried out on 96-cell plastic flat-bottom tablet. Erythrocyte suspension and immunospecific sera are introduced into the cells, shaken, placed into thermostat and reading data. The tablets are shaken with tablet-type photometer. Visible light absorption capacity in bandwidth of 630-650 nm is determined in samples by means of the tablet-type photometer before placing the tablet into thermostat. The samples are incubated at 37°C in thermostat for 40 min. Then, the samples are additionally shaken and incubated for more 1.5-2.0 h, and visible light absorption capacity in bandwidth of 630-650 nm is determined once more. The reaction readings are taken by subtracting visible light absorption indices of the samples after incubation from those before incubation. The received values are transformed into serologic test points.

EFFECT: high estimation accuracy; facilitated animal testing process.

6 tbl

FIELD: poultry farming.

SUBSTANCE: method involves addition of lithium citrate in the dose 30-35 mg/kg of live mass to water used for watering poultry 24 h before the stress effect. Method provides enhancing safety and productivity of poultry.

EFFECT: improved method of correction.

11 tbl

FIELD: animal science.

SUBSTANCE: one should supplement the feedstuff with Phosprenyl, at the dosage of 0.05 ml/kg by the weight of offspring at whelping - once; in case of lactation and before weaning the whelps - twice or thrice. The innovation increases the offspring output, the safety of offspring, increases body weight and body size and quality of skins.

EFFECT: higher efficiency.

6 tbl

FIELD: agriculture.

SUBSTANCE: method involves introducing copepods (Acanthocyclops viridis, Eucycllops serrulatus, Cyclops strennus) and Cladocera (Daphnia magna, Daphnia pulex) crayfish at copepods and Cladocera ratio of 1:1 in amounts providing population density of 30-50 spc/l of water of biotope of mollusks-alternate host of common fasciola.

EFFECT: increased efficiency in fascioliasis prophylaxis and ecologically clean method.

1 tbl, 9 ex

FIELD: animal farming.

SUBSTANCE: method involves determining build indexes; taking animal's measurements six times per day in equal time intervals; calculating chest index on the basis of measurements obtained, said index being "activation" element in "well-proportion" system, and its summing elements such as outgrowth index, long-leg index and lengthiness index; for stoutness system, setting introduction elements such as "meatness" and "boneness" indexes, and its summing functioning elements such as "well-knitness" and "broad-bodyness" indexes; determining animal's build type on the basis of equations established by minimal quadrate method, said quadrates being defined from build indexes calculated from taken daily measurements; acknowledging animals as milky type when they have "activation" element in "lengthiness" system and its summing element expressed in reliable logarithmic equation and in "stoutness" system expressed in linear equation; acknowledging animals as meaty-milky type when "activation" element in "lengthiness" system is expressed by parabola, its summing element is expressed by linear equation, and in "stoutness" system it is expressed by linear equation and parabola; acknowledging animals as meaty type when animals' "activation" element in "lengthiness" system is expressed by linear equation, its summing element is expressed by parabola, and in "stoutness" system it is expressed by equation of parabola and exponent.

EFFECT: increased accuracy and fastness in determining type of cattle animal's build.

19 dwg, 18 tbl, 1 ex

FIELD: animal science.

SUBSTANCE: the present innovation deals with dynamic loading onto cardio-vascular system in animals. Selection should be carried out by the following parameters: , ΔT3 and Δn, where ΔT1 - the time for pulse increase at running, ΔT2 - the time for pulse stabilization after running, ΔT3 - the time for pulse increase after running, Δn - the increase of pulse frequency after running. One should select animals into milking herd at the following values; ΔT3 ≤ 10 sec, Δn ≤ 10 beats/min. The method enables to present perspective evaluation of lactation capacity in animals.

EFFECT: higher efficiency of selection.

1 dwg, 1 ex, 1 tbl

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