Method for purifying 5-aminolevulinic 5-amino-4-oxopentaenic) acid hydrochloride

FIELD: organic chemistry, biochemistry, chemical technology.

SUBSTANCE: invention relates to a method for purification of synthetic 5-aminolevulinic (5-amino-4-oxopentaenic) acid hydrochloride (5-ALA) representing endogenous substance and biological precursor of porphyrines in living organism and plants. Preliminary purification of 5-ALA hydrochloride is carried out by electrodialysis of 5-ALA solutions with the concentration 140-300 g/l in current density 0.5-2.0 A/dm2 and at temperature 20-30°C. Proposed method of purification of 5-ALA hydrochloride provides isolating expensive preparation from filtrates and to enhance the content of basic substance in technical 5-ALA hydrolyzate prepared by catalytic and electrochemical methods with the parent content of basic substance 87.0%, not above, that results to enhancing yield of final 5-ALA hydrochloride. Purified substance can be used in research and industrial practice.

EFFECT: improved purifying method.

8 ex

 

The present invention relates to a method of cleaning a synthetic hydrochloride of 5-aminolevulinic (5-amino-4-oxopentanoate) acid (ALA) is an endogenous amino acids, which is the biological precursor of porphyrins in all living organisms, including humans, and can be used in research and industrial practice.

The ability of ALA to turn into tumor cells in protoporphyrin lX (photosensitizer that generates singlet oxygen upon irradiation with visible light) is widely used in medicine for photodiagnosis and photodynamic therapy of malignant tumors of different localization, as well as for the treatment of skin diseases non-neoplastic nature [Q.Peng, Kogd, J.Moan et al. Photochem. Photobiol. 1997, 65, 235-251]. ALA is used as an immunostimulant [RF Patent 2160587, a 61 K 31/195, 2000], as well as in agriculture as a growth stimulant plants, herbicide and other [European patent EP 514776, 12 P 13/00, 1992]. A wide range of use ALA is of great interest to its production in many countries of the world.

A known number of synthetic methods for obtaining ALA from various types of raw materials. So, one of the most frequently used for ALA synthesis of the compounds is aired 5-problevpontai acid, in which the replacement of the bromine on the amino group is carried out either by the action of phthalimide with the respective hydrolysis, either through the stage of receipt of the corresponding azide, followed by reduction [..Morton, M.E.Leanna Tetrahedron Lett. 1993, 34 (28), 4481-4484]. In recent years there have been publications on the synthesis of ALA derivatives of pyridine, piperidine, furan, tetrahydrofuran (THF) [European patent 718405, 12 P 13/00, 1996]. However, all these methods either ethnologica and time-consuming, or require the use of hard-to-reach source of substances, while the outputs of ALA in them low, which hampers their development in the industry.

In a number of patents as a source of the substance to obtain ALC using 5-nitrocelulose (5-nitro-4-oxopentanoate) acid (NLC) or its esters, nitro-group which restores chemically or electrochemically. Thus, a method of obtaining ALA the hydrogenation diluted (0.18 to 1.5%) solutions of NLC in the environment 2M hydrochloric acid on the catalyst 10% Pd/C at a temperature of 20÷110°and a hydrogen pressure of 1-3 at [JP 09316041, C 07 C 229/22, 19.12.97].

In a later patent, the starting material is methyl ether NLC, which hydronaut catalyst 5% Pd/C in acidic methanolic medium at a temperature of 5-30°and pressure of 10-20 at [RF Patent 2146667, C 07 C 229/22, 2000]. The authors have defined the content of the basic substance in the technical ALA obtained by this method is 89-96%. Also tested the method of purification of ALA, proposed and selected as p is of ototype.

This method of cleaning is to recrystallization ALA, which is dissolved by heating in hydrochloric acid (1:1), treated with activated charcoal, the solution is filtered, the filtrate contribute in acetone, precipitated precipitate is filtered off and dried. The authors have shown that to achieve the content of the basic substance 98-99% requires one recrystallization procedure in the case where the content of ALA in the sample is not lower than 93%, can be achieved 70-75% weight yield. At a lower content of ALA in the cleaned sample to achieve the same quality indicators requires more operations recrystallization, and the output of the purified substances significantly reduced. Thus, when the content of ALA in the original sample 90-92% requires two successive recrystallization when the total output 40-45%, and when the content of ALA in the original sample 86-89% required three recrystallization when the total output of 25-30%.

However, this method of purification was not possible to improve the quality of ALA content below 85%, because in these cases there is a decrease in the content of ALA.

At the same time, there is an urgent need for clean samples of ALA with a low content of basic substance. ALA this quality is formed, for example, processing of the manifold is formed by the above procedure, the cleaning ALA. the ri receiving ALA electrochemical recovery of the methyl ester NLC on graphite cathode in hydrochloric acid aqueous solution at a temperature of 60-75° C and current density of 2-10 A/DM2[RF patent 2260585, C 07 C 229/22, 2005] the content of the basic substance is also low and is 70-80%.

With the aim of developing a method of purifying low-ALC identified and described the main impurities contained therein. They were ammonium chloride and methylammonium chloride in the ratio of ˜4:1. Apparently, these impurities are formed during the catalytic or electrochemical reduction products of the side reactions occurring cleavage characteristic of aliphatic □-nitroethanol [Nielsen, A.T. Nitronovoe acids and esters. In : "Chemistry of the nitro and microsorum" under the editorship of Foyer,, M.: Mir, 1972, volume 1, str-370].

The task of the invention is the cleaning of the ALA, the resulting recovery (catalytic or electrochemical) derived NLC. To solve this problem, a method in which cleaning low-ALK carry out the electrodialysis 14-30% solutions of ALA at current density of 0.5 to 2.0 a/DM2and a temperature of 20-30°allowing to improve the content of the basic substance to 92-97%. Subsequent recrystallization of the prototype increases the content of the basic substance to 98-99%.

Data electrodialysis purification of solutions of 5-ALA from inorganic impurities in the literature are absent, but there is information [Japan's Bid 61-44188, With 25 In 3/00, 1986; RF patent 206612, 07 With 309/14, 1996] purification by electrodialysis other amino acids, which in solution are in the form of neutral inner salt, which simplifies the cleaning process. ALA in the solution is in the ionized state, which complicates the cleaning of electrodialysis method.

The process of electrodialysis purification solutions ALC is carried out in a multichamber apparatus-electrodialyzer at current density of 0.5 to 2.0 a/DM2temperature 20-30°and concentration on the base material 14-30%.

The increase in current density above 2.0 a/DM2impractical because it leads to lower output of the ALC and the increasing intensity of the process. At the same time at current densities below 0.5 a/DM2reduced productivity of the process.

For similar motifs and the range of operating concentrations above 30% reduced yield purified ALA, and below 14% increase in energy consumption for removal of solvent.

The upper boundary of the temperature range at which carry out the process of electrodialysis purification of ALA in accordance with the invention is limited by thermal stability of the anion-exchange membranes, the lower the decrease in the conductivity of the solution.

The allocation of purified ALA of the solution is carried out by removing the solvent under vacuum, the introduction of the residue in acetone, allocation filtering of education is on the precipitate and drying. The proposed method from samples with low ALA content of 70-85% receive ALA content of the basic substance is not less than 92% and the output 56-75%. Subsequent recrystallization method, described above, leads to samples with ALA content of the basic substance is not less than 98%.

The following examples illustrate the invention.

Example 1 (the prototype)

In a three-neck flask equipped with a stirrer and a jacket for temperature control, load 39,55 g ALA content of 74% (isolated from the filtrate recrystallization technical ALA)to 39.5 ml of hydrochloric acid (1:1) and stirred until complete dissolution of ALA when heated to 70-85°C. At this temperature, to the resulting solution was added 1.5 g of activated carbon and stirred for 30-45 minutes. Then the solution is filtered warm for exemption from coal, the resulting filtrate contribute in acetone and stirred. The formed precipitate is filtered off, washed with acetone and dried. Get 19,8 g ALA content of the main substance of 68% and a yield of 46% per 100% of the product.

Repeated recrystallization leads to obtain 11.1 g of ALA content of the basic substance 63,3% and yield of 52% per 100% of the product.

Further purification of ALA by recrystallization leads to obtain 6.0 g of ALA content of the basic substance 57.4% and a yield of 49% per 100 product.

Example 2

A solution of ALA was obtained by dissolving 163 g of the sample with ALA content of the basic substance 70% in 300 ml of distilled water, subjected to electrodialysis purification in a multi-chambered apparatus-electrodialyzer filter presnogo type, consisting of alternating membranes marks MK-40 and MA-40 with the intermediate part of paronite and separators - energizers. The cathode is a plate of stainless steel 18CR10NITI with the working surface 1,0 DM2, the anode platinized titanium with the same surface. Electrodialyzer consists of 6 cameras "clean" and 7 cameras "concentration", and two electrode chambers. The working surface of each membrane 1,0 DM2and the distance between them is 1.5 mm Solution of ALA pump pumped through the camera "clean", at the same time through the camera "concentration" pumped distilled water, and through the electrode chambers - tap water. When the temperature of the solution ALA 20°through electrodialyzer miss DC power which corresponds to a current density of 2.0 a/DM2. The temperature during the electrodialysis is performed by the supply of cold water in the jacket of the intermediate containers. In the process of electrodialysis purification receive 478 ml of purified solution of ALA, which under reduced pressure, distilled water. The obtained residue contribute to ACE is he, the precipitation is filtered off, washed with acetone and dried. Get 66 g ALA content of the basic substance to 92% (output substance 53%, the current output 71%).

Example 3

A solution of ALA was obtained by dissolving 88 g of the sample with ALA content of the basic substance 77% in 350 ml of distilled water, subjected to electrodialysis purification and separation of purified ALA analogously to example 2. Get to 44.9 g of product with a basic substance content of 96% (the substance 64%, the output current 72%).

Example 4

A solution of ALA was obtained by dissolving 73 g of the sample with ALA content of the basic substance of 87% in 400 ml of distilled water, subjected to electrodialysis purification and separation of purified ALA analogously to example 2, but at current density of 0.5 a/DM2. Get to 46.8 g of product with a basic substance content of 95% (the substance of 70%, the current efficiency of 67%).

Example 5

A solution of ALA was obtained by dissolving 73 g of the sample with ALA content of the basic substance of 87% in 400 ml of distilled water, subjected to electrodialysis purification and separation of purified ALA analogously to example 2, but at the current density of 1.0 A/DM2and a temperature of 30°C. Obtain 43 g of the product with the content of the main substance of 97%. (the substance of 66%, the current output 73%).

Example 6

A solution of ALA was obtained by dissolving 124 g of the sample with ALA content of the basic substance 85 230 ml of distilled water, subjected to electrodialysis purification and separation of purified ALA analogously to example 2. Obtain 71 g of the product with the content of the basic substance to 92% (output substance 62%, the current output 54%).

Example 7 (comparative)

A solution of ALA was obtained by dissolving 139 g of the sample with ALA content of the basic substance 85% in 210 ml of distilled water, subjected to electrodialysis purification and separation of purified ALA analogously to example 2, but at the current density of 3.0 A/DM2. Get to 60.6 g of product with a basic substance content of 92% (output substance 47%, the output current 44%).

Example 8

Cleanse 66 g ALA content of the basic substance 92% (example 2) described in example 1, the process of recrystallization. Get 43,6 g purified ALA content of the basic substance to 98.4% (71% yield according to the stage of recrystallization).

Similarly, samples from ALA, obtained in examples 3-6, with high yields (65-75%) receive samples with the content of the basic substance 98-99%.

Thus, the proposed cleaning method allows to increase the content of the basic substance in the technical samples ALA and using crystallization, to increase the total yield of the target product with the required content of the basic substance 98-99%.

The method of purification of the hydrochloride of 5-aminolevulinic (5-amino-4-oxopentanoate) acids, including the cross is stallization product, characterized in that before recrystallization spend cleaning solution by electrodialysis at current density of 0.5 to 2.0 a/DM2temperature 20-30°and the concentration of the basic substance 14-30%.



 

Same patents:

FIELD: organic chemistry, medicine, pharmacy.

SUBSTANCE: invention relates to physiologically acceptable L-carnitine and alkanoyl L-carnitine salts characterizing by nonhygroscopicity and stability used in preparing solid formulations and suitable for oral using. Invention proposes L-carnitine or alkanoyl L-carnitine salt with taurine chloride represented by the general formula (I): wherein R represents hydrogen atom or lower linear or branched alkanoyl comprising from 2 to 5 carbon atoms. Invention provides the development of stable nonhygroscopic L-carnitine and lower alkanoyl L-carnitine salts that are valuable supplements in human diet nutrition and animals under normal physiological state and good health, and also in low absorption syndrome occurring in children and adults, and in the development of the food composition supplement.

EFFECT: improved and valuable properties of compositions.

8 cl, 2 ex

FIELD: organic chemistry, chemical technology, medicine.

SUBSTANCE: invention proposes a method for preparing 5-aminolevulinic acid (ALA) alkyl esters and their hydrochlorides. Method for preparing ALA alkyl ester hydrochlorides involves the esterification reaction of ALA hydrochloride with primary or secondary (C1-C6)-alcohols in the presence of a heterogenous acid catalyst wherein cation-exchange resins in H+-form can be used as a catalyst with their preliminary dehydration by thermal treatment or azeotropic distillation off. Invention provides preparing indicated compounds by development of ecologically safe, technologically effective and economy method. ALA alkyl esters are used in fluorescent diagnosis and photodynamic therapy of malignant tumors of different localization and in treatment of cutaneous diseases of nontumor nature.

EFFECT: improved preparing method.

7 ex

FIELD: organic chemistry, chemical technology, medicine.

SUBSTANCE: invention relates to a method for preparing the synthetic 5-aminolevulinic (5-amino-4-oxopentanoic) acid hydrochloride (5-ALA) representing an endogenous substance (metabolite). Method for preparing 5-ALA involves electrochemical reduction of 5-nitrolevulinic acid methyl ester in acid aqueous medium on graphite cathode at temperature 60-75°C and the current density value 2-10 A/dm2. 5-ALA can be used in medicine for photodiagnosis and photodynamic therapy of malignant tumors of different localization and for treatment of diseases of non-tumor nature also. Invention provides simplifying technology of synthesis of 5-ALA, improved economy indices and provides ecological safety of the process.

EFFECT: improved preparing method.

4 ex

FIELD: organic chemistry, medicine.

SUBSTANCE: invention relates to compounds designated for applying in photochemotherapy or diagnosis and indicated compounds represent 5-aminolevulinic acid aryl-substituted esters, their derivatives or pharmaceutically acceptable salts. In particular, invention provides preparing compounds of the general formula (I): R

22
N-CH2COCH2CH2CO-OR1 wherein R1 represents aryl-substituted C1-alkyl group, preferably C1-alkyl group substituted with non-heteroaromatic aryl wherein indicated group aryl is substituted group, and especially preferable this radical is substituted with one or more alkyl groups (for examples, (C1-C2)-alkyl), alkoxy- (for example, methoxy-) groups, fluorine, chlorine atoms, nitro- or trifluoromethyl groups; R2 being each of that can be similar or different represents hydrogen atom or alkoxycarbonyloxy-; indicated alkyl group is broken optionally with one or more groups: -O-, -NR3-, -S- or -PR3- wherein R3 represents hydrogen atom or (C1-C6)-alkyl group, and their salts for applying in diagnosis and photochemotherapy of injures and disorders of internal and external surfaces of body, and products and sets for realization of this invention also.

EFFECT: valuable medicinal properties of compounds.

18 cl, 17 dwg, 2 tbl, 3 ex

The invention relates to new N-[alkylphenoxy(ethyleneoxy)carbonylmethyl] ammonium chlorides of General formula (1), which can be used to improve the rheological properties of oil dispersion in the oil and petrochemical industry, where R is alkyls8-C10, R1= R2= -CH2CH2HE, R3represents a group of formula (2), in which R4= alkyls15-C25where n is the average degree of oksietilirovannye equal to 10; R = alkyls8-C10, R1=CH2CH2HE, R2=R3and represent a group of formula (2), where R4= alkyls15-C25, n is the average degree of oksietilirovannye equal to 10; R = alkyls8-C10, R1=R2=H, R3= alkyls10-C16, n is the average degree of oksietilirovannye equal to 6; with the properties of the additives governing viscoelastic properties associated multicomponent oil systems

The invention relates to new N,N-dimethyl-N-alkyl-N-[alkoxyl(ethylenoxy)carbonylmethyl] ammonium chlorides of General formula (1), where R1, R2- aliphatic hydrocarbon radical containing 10 to 16 carbon atoms; n is the average degree of oksietilirovannye equal to 3, having a fungistatic and bactericidal activity, and which additives governing viscoelastic properties associated multicomponent oil systems

The invention relates to new N-[Alkoxyl(ethylenoxy)carbonylmethyl] ammonium chlorides of General formula (1), which can be used in the oil and petrochemical industries, where R is an aliphatic hydrocarbon radical containing 12 to 16 carbon atoms; n is the average degree of oksietilirovannye equal 3-4; R1=R2= -CH2CH2HE; R3represents a group of formula (2), where R4- aliphatic hydrocarbon radical containing 15-25 carbon atoms having the properties of additives governing viscoelastic properties associated multicomponent oil systems

The invention relates to N-[alkoxyl(ethylenoxy)carbonylmethyl]ammonium chlorides of the formula (I) having fungistatic and bactericidal activity, and method of production thereof

The invention relates to a method for producing synthetic hydrochloride of 5-aminolevulinic (5-amino-4 - oxopentanoate) acid formula HCLH2NCH2COCH2CH2COOH

FIELD: analytical methods.

SUBSTANCE: method of determining glycine when developing continuous protein fermentation processes consisting in preparing aqueous solution of glycine followed by extraction and further analysis of organic phase is characterized by that 20-35% by weight of lithium sulfate as salting-out agent is added to aqueous solution of glycine (pH˜5) and then solution is extracted with ternary mixture of hydrophilic solvents: 65-65.5% isopropyl alcohol, 20-20.5% acetone, and 14-15% ethyl acetate at volume ratio of extractant mixture to aqueous salt solution 1:10. Nonaqueous concentrate is further analyzed using potentiometric titration technique.

EFFECT: increased degree of glycine recovery to 98-99% and avoided use of dangerous reagents in organic phase analysis.

1 tbl, 11 ex

FIELD: analytical methods.

SUBSTANCE: method of determining glycine when developing continuous protein fermentation processes consisting in preparing aqueous solution of glycine followed by extraction and further analysis of organic phase is characterized by that 20-35% by weight of lithium sulfate as salting-out agent is added to aqueous solution of glycine (pH˜5) and then solution is extracted with ternary mixture of hydrophilic solvents: 65-65.5% isopropyl alcohol, 20-20.5% acetone, and 14-15% ethyl acetate at volume ratio of extractant mixture to aqueous salt solution 1:10. Nonaqueous concentrate is further analyzed using potentiometric titration technique.

EFFECT: increased degree of glycine recovery to 98-99% and avoided use of dangerous reagents in organic phase analysis.

1 tbl, 11 ex

FIELD: organic chemistry, biochemistry, chemical technology.

SUBSTANCE: invention relates to a method for purification of synthetic 5-aminolevulinic (5-amino-4-oxopentaenic) acid hydrochloride (5-ALA) representing endogenous substance and biological precursor of porphyrines in living organism and plants. Preliminary purification of 5-ALA hydrochloride is carried out by electrodialysis of 5-ALA solutions with the concentration 140-300 g/l in current density 0.5-2.0 A/dm2 and at temperature 20-30°C. Proposed method of purification of 5-ALA hydrochloride provides isolating expensive preparation from filtrates and to enhance the content of basic substance in technical 5-ALA hydrolyzate prepared by catalytic and electrochemical methods with the parent content of basic substance 87.0%, not above, that results to enhancing yield of final 5-ALA hydrochloride. Purified substance can be used in research and industrial practice.

EFFECT: improved purifying method.

8 ex

FIELD: chemistry.

SUBSTANCE: anhydride is a physiologically active substance and can be used, for example, in chemotherapy as a low-toxic agent for inhibiting growth of carcinoma 755 (breast cancer). The object of the present invention is synthesis of a novel mixed anhydride based on dichloroacetic and aminoacetic acid which, for instance, enables to inhibit growth of carcinoma 755 (breast cancer) in monotherapy. The given task is solved through synthesis of a novel mixed anhydride based on dichloroacetic acid and aminoacetic acid of formula 1, which can be used in medical practice as an anti-tumour agent which enables, for example, to inhibit growth of carcinoma 755. The other objective of the invention is designing a method of producing the mixed anhydride based on dichloroacetic acid and aminoacetic acid. This task is solved using a method which involves successive reaction of aminoacetic acid with an alkali metal hydroxide in an aqueous medium followed by treatment of the reaction mass with dichloroacetyl chloride in a chloroalkane solution, acidation of the reaction medium with aqueous hydrochloric acid solution and extraction of the end product using existing techniques. The disclosed compound can be used in medical practice as an anti-tumour compound.

EFFECT: use of said compound in oncological practice inhibits growth of carcinoma.

2 cl, 1 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to a method for preparing a solid form of the compound gadobenate dimeglumine of formula (I) used as a contrast agent for diagnostic visualisation, particularly in magnetic resonant tomography. The presented method involves spray drying of a liquid composition of the above compound. Preferentially, the liquid composition represents an aqueous solution. The invention also refers to a solid powder form of gadobenate dimeglumine prepared by the method described above, a pharmaceutical kit containing this form, and a method for preparing a solid form of 4-carboxy-5,8,11-tris(carboxymethyl)-1-phenyl-2-oxa-5,8,11-triazatridecane-13 acid.

EFFECT: method enables preparing the high-yield and reproducible solid form of gadobenate dimeglumine, which possesses good flowability and dissolution rate.

15 cl, 3 ex

FIELD: organic chemistry, medicine.

SUBSTANCE: invention relates to compounds designated for applying in photochemotherapy or diagnosis and indicated compounds represent 5-aminolevulinic acid aryl-substituted esters, their derivatives or pharmaceutically acceptable salts. In particular, invention provides preparing compounds of the general formula (I): R

22
N-CH2COCH2CH2CO-OR1 wherein R1 represents aryl-substituted C1-alkyl group, preferably C1-alkyl group substituted with non-heteroaromatic aryl wherein indicated group aryl is substituted group, and especially preferable this radical is substituted with one or more alkyl groups (for examples, (C1-C2)-alkyl), alkoxy- (for example, methoxy-) groups, fluorine, chlorine atoms, nitro- or trifluoromethyl groups; R2 being each of that can be similar or different represents hydrogen atom or alkoxycarbonyloxy-; indicated alkyl group is broken optionally with one or more groups: -O-, -NR3-, -S- or -PR3- wherein R3 represents hydrogen atom or (C1-C6)-alkyl group, and their salts for applying in diagnosis and photochemotherapy of injures and disorders of internal and external surfaces of body, and products and sets for realization of this invention also.

EFFECT: valuable medicinal properties of compounds.

18 cl, 17 dwg, 2 tbl, 3 ex

FIELD: organic chemistry, chemical technology, medicine.

SUBSTANCE: invention relates to a method for preparing the synthetic 5-aminolevulinic (5-amino-4-oxopentanoic) acid hydrochloride (5-ALA) representing an endogenous substance (metabolite). Method for preparing 5-ALA involves electrochemical reduction of 5-nitrolevulinic acid methyl ester in acid aqueous medium on graphite cathode at temperature 60-75°C and the current density value 2-10 A/dm2. 5-ALA can be used in medicine for photodiagnosis and photodynamic therapy of malignant tumors of different localization and for treatment of diseases of non-tumor nature also. Invention provides simplifying technology of synthesis of 5-ALA, improved economy indices and provides ecological safety of the process.

EFFECT: improved preparing method.

4 ex

FIELD: organic chemistry, chemical technology, medicine.

SUBSTANCE: invention proposes a method for preparing 5-aminolevulinic acid (ALA) alkyl esters and their hydrochlorides. Method for preparing ALA alkyl ester hydrochlorides involves the esterification reaction of ALA hydrochloride with primary or secondary (C1-C6)-alcohols in the presence of a heterogenous acid catalyst wherein cation-exchange resins in H+-form can be used as a catalyst with their preliminary dehydration by thermal treatment or azeotropic distillation off. Invention provides preparing indicated compounds by development of ecologically safe, technologically effective and economy method. ALA alkyl esters are used in fluorescent diagnosis and photodynamic therapy of malignant tumors of different localization and in treatment of cutaneous diseases of nontumor nature.

EFFECT: improved preparing method.

7 ex

FIELD: organic chemistry, medicine, pharmacy.

SUBSTANCE: invention relates to physiologically acceptable L-carnitine and alkanoyl L-carnitine salts characterizing by nonhygroscopicity and stability used in preparing solid formulations and suitable for oral using. Invention proposes L-carnitine or alkanoyl L-carnitine salt with taurine chloride represented by the general formula (I): wherein R represents hydrogen atom or lower linear or branched alkanoyl comprising from 2 to 5 carbon atoms. Invention provides the development of stable nonhygroscopic L-carnitine and lower alkanoyl L-carnitine salts that are valuable supplements in human diet nutrition and animals under normal physiological state and good health, and also in low absorption syndrome occurring in children and adults, and in the development of the food composition supplement.

EFFECT: improved and valuable properties of compositions.

8 cl, 2 ex

FIELD: organic chemistry, biochemistry, chemical technology.

SUBSTANCE: invention relates to a method for purification of synthetic 5-aminolevulinic (5-amino-4-oxopentaenic) acid hydrochloride (5-ALA) representing endogenous substance and biological precursor of porphyrines in living organism and plants. Preliminary purification of 5-ALA hydrochloride is carried out by electrodialysis of 5-ALA solutions with the concentration 140-300 g/l in current density 0.5-2.0 A/dm2 and at temperature 20-30°C. Proposed method of purification of 5-ALA hydrochloride provides isolating expensive preparation from filtrates and to enhance the content of basic substance in technical 5-ALA hydrolyzate prepared by catalytic and electrochemical methods with the parent content of basic substance 87.0%, not above, that results to enhancing yield of final 5-ALA hydrochloride. Purified substance can be used in research and industrial practice.

EFFECT: improved purifying method.

8 ex

Up!