Method for preparing agent for maintaining therapy possessing tissue-specific activity

FIELD: medicine, peptides.

SUBSTANCE: invention relates to a method for preparing a peptide complex from animal raw possessing the tissue-specific activity. Method for preparing an agent for maintaining therapy possessing tissue-specific activity involves milling calf or pig organs of age 12 months, not above, addition of 3% acetic acid solution at temperature 20 ± 5°C and extraction is carried out at constant stirring. Then, in 30 min zinc chloride 1% solution is added, mixture is cooled to temperature 7-16°C at constant stirring followed by stirring for every 1 h in each 4 h and settling for 48 h. Extract is separated from inert substances by separation and acetone is added to extract in the volume ratio = 1:5, mixture is kept at 3-5°C for 4 h and formed precipitate is washed out with out with two-fold volume of acetone cooled to temperature 7-16°C, formed precipitate is rubbed through a metallic sieve and prepared end product is dried at temperature 18 ± 2°C. The end product represents a peptide complex with the content of low-molecular peptide fraction from 70% to 90% and with molecular mass of its peptide components in the range 1000-12000 Da wherein this complex comprises amino acids, mineral substances, trace elements and vitamins in biologically bound form. The complex elicits the expressed tissue-specific activity based on the proposed sequence of technological procedures and conditions for their realization involving temperature, temporal and other indices, and by using substances including the parent raw, a definite extractant and others. Peptide component in the prepared complex has no denaturating properties and retains its regulatory properties that suggests its using as an agent for carrying out the maintaining therapy. The proposed agent can be used in medicinal practice as an agent used in carrying out the maintaining therapy.

EFFECT: improved preparing method, valuable medicinal properties of agent.

9 cl, 42 tbl, 14 ex

 

The invention relates to medicine and the receipt from animal products peptide complex with tissue-specific activity, which may find application in medical practice as a means for supporting therapy.

A method of obtaining peptides from animal raw materials, namely of the epiphysis of cattle (RF patent No. 2136296, 1997), which are crushed, extracted in a solution of acetic acid, frozen at minus 5-20°and thawed, and then pre-clarified extract is subjected to ultrafiltration in a tangential flow membranes with a threshold bandwidth 10 KD. Yield of target product per unit of raw material is 12 g per 1 kg of raw material. The resulting peptide complex according to the method described in patent RF №2136296, contains no protein contamination (negative reaction on the protein with trichloroacetic acid, and data high-performance liquid chromatography), meets the criteria of authenticity (the maximum absorption in the UV range is 270±5 nm) and has a specific activity.

A method of obtaining a peptide complex from the pineal gland of cattle (RF patent No. 944191, 1994), including extraction of the crushed material 3-5% acetic acid with addition of chloride of zinc, zentrifugenbau is s, the deposition of the target product from the supernatant six volumes of acetone and ether, renaturation in 1%acetic acid, followed by clarification and preparation of dosage forms.

However, obtained in this way peptide complex contains protein contamination, and the output of the target product per unit of raw material is small and is 7 g/kg

The closest technical solution is a method of obtaining from animal raw materials with tissue-specific action nucleoprotein complex (patent RF №2075944, 1996) with molecular Masaya member nucleoprotein components from 10000 to 90000 Yes.

The method described in the patent of Russian Federation №2075944, provides for the extraction of pre-prepared (chopped and frozen) animal raw materials using as extractant 1-2,5% solution of sodium carbonate (soda) at a pH of 10.4 and 10.8 for 2-4 h with the addition of 0.1-0.5%magnesium chloride at a ratio of volumes of the extracted tissue and soda solution in the range of 1:8-1:20, separating the precipitate by filtration or centrifuge, the acidification of the extract to a pH of 3.8 to 5.5, cooling the suspension at a temperature not exceeding 4°C for 40 h to remove insoluble substances, washing the precipitate with an organic solvent (successively with ethanol, acetone, ethyl ether, drying the precipitate in a vacuum is at a temperature not exceeding 55° C.

The yield of the target product (powder nucleoprotein complex) is 2.5-3.0% of quantity of raw materials.

It should be noted that although the target product, obtained by the method described in the patent of Russian Federation №2075944 contains nucleoprotein complexes with specificity of action and trapnest in relation to organs and tissues that are the source of their reception, however, the known method has several disadvantages, among which it is necessary to note the following.

Powder nucleoprotein complex of organs and tissues obtained in the described manner, refers to a group of poorly soluble substances, which requires specific techniques for use in medical practice.

The resulting product consists of two groups of molecular associates: a sufficiently large number of high molecular weight components, including proteins of class amyloid, composed of prions (mol. wt. from 27000 to 31000 Yes), and nucleic acid component, which carries the danger of the presence of the target proto-oncogene product.

The problem to which the invention is directed, is the development of technologies for extraction of purified protein, nucleic acid and lipid impurities of low molecular weight complex, the peptide component which is not denaturised and who preserved their regulatory properties.

The technical result is that the proposed method are peptide complex with a molecular mass of its constituent peptide components from 1000 to 12000 Yes, contains amino acids, minerals, trace elements and vitamins in biologically bound form, showing a distinct tissue-specific activity of the resulting peptide component of the received complex denaturised and retains its regulatory properties, which suggests its use as a tool for maintenance therapy.

The method of obtaining funds for maintenance therapy with tissue-specific activity, includes grinding raw materials of animal origin, the extraction was carried out 3%solution of acetic acid with the addition of zinc chloride, cooled to a temperature of plus 7-16°With constant stirring for 48 h, the separation of the extract from ballast substances separation, sedimentation, which is carried out with acetone in a volume ratio of 1:5 at a temperature of plus 3-5°C for 4 h, the defending formed before the formation of sludge, which is then washed with two volumes of acetone, washed precipitate wipe through metal sieve obtained target product is dried at a temperature of 18±2°to complete taleniekov acetone.

At the same time as the target product is obtained peptide complex with a molecular mass of its constituent peptide components in the range from 1000 to 12000 Yes.

At the same time as animal feedstock use liver, and peptide complex, supports liver function, contains amino acids, minerals, trace elements and vitamins in biologically bound form in the following ratio, wt.%:

amino acids65,0-75,0
minerals3,0-5,0
trace elements0,003-0,008
vitamins0,01-0,03

At the same time as animal feedstock use pancreas, and peptide complex, supports the function of the pancreas, contains amino acids, minerals, trace elements and vitamins in biologically bound form in the following ratio, wt.%:

amino acids45,0-55,0
minerals10,0-15,0
trace elements0,001-0,003
vitamins0,005-0,010

At the same time as animal feedstock use of a thyroid gland, and the peptide is the first complex, supporting the function of the thyroid gland, contains amino acids, minerals, trace elements and vitamins in biologically bound form in the following ratio, wt.%:

amino acids70,0-80,0
minerals3,0-5,0
trace elements0,002-0,004
vitamins0,001-0,003

At the same time as animal raw materials used vessels, and peptide complex, supports the function of blood vessels, contains amino acids, minerals, trace elements and vitamins in biologically bound form in the following ratio, wt.%:

amino acids80,0 to 90.0
minerals3,0-5,0
trace elements0,005-0,010
vitamins0,001-0,003

At the same time as animal feedstock using cartilage and peptide complex, supports cartilage, contains amino acids, minerals, trace elements and vitamins in biologically bound form in the following ratio, wt.%:

aminos is slots 50,0-60,0
minerals7,0-9,0
trace elements0,002-0,008
vitamins0,001-0,003

At the same time as animal feedstock use the brain, and peptide complex, supports the function of the brain, contains amino acids, minerals, trace elements and vitamins in biologically bound form in the following ratio, wt.%:

amino acids50,0-60,0
minerals4,0-6,0
trace elements0,002-0,004
vitamins0,001-0,003

At the same time as animal feedstock use the thymus, and peptide complex, supports the immune system, contains amino acids, minerals, trace elements and vitamins in biologically bound form in the following ratio, wt.%:

amino acids55,0-65,0
minerals3,0-5,0
trace elements0,001-0,003
vitamins0,010-0,030

Thus the maximum absorption of the target product ultravioleta spectrum (270± 5) nm.

The proposed technology in contrast to the known allows you to get cleared nucleic acid, protein and lipid impurities peptide complex with a molecular mass of its constituent peptide components from 1000 to 12000 Yeah, containing biologically bound form trace elements, mineral substances and vitamins, has expressed tissue-specific activity, which is achieved by the proposed process sequence and the conditions of their implementation, including temperature, time and other characteristics, and substance use, including raw materials, specific extractant and other

The invention is illustrated by tables.

Table 1 presents the amino acid composition and ratio of amino acids included in the peptide complex isolated from the liver.

Table 2 presents the content of mineral substances in peptide complex isolated from the liver.

Table 3 presents the elemental composition of the peptide complex isolated from the liver.

Table 4 presents the results of the content of vitamins in peptide complex isolated from the liver.

Table 5 presents the chemical composition of the peptide complex isolated from the liver.

Table 6 shows the effect of peptide complex isolated from the liver, the development of explants is s liver.

Table 7 presents the amino acid composition and ratio of amino acids included in the peptide complex isolated from the pancreas.

Table 8 presents the content of mineral substances in peptide complex isolated from the pancreas.

Table 9 presents the elemental composition of the peptide complex isolated from the pancreas.

Table 10 presents the content of vitamins in peptide complex isolated from the pancreas.

Table 11 presents the chemical composition of the peptide complex isolated from the pancreas.

Table 12 shows the effect of peptide complex isolated from the pancreas, on the development of explants of the pancreas.

Table 13 shows the amino acid composition and ratio of amino acids included in the peptide complex isolated from the thyroid gland.

Table 14 shows the content of mineral substances in peptide complex isolated from the thyroid gland.

Table 15 presents the elemental composition of the peptide complex isolated from the thyroid gland.

Table 16 presents the vitamin composition of the peptide complex isolated from the thyroid gland.

Table 17 presents the chemical composition of the peptide complex isolated from the thyroid gland is s.

Table 18 shows the effect of peptide complex isolated from the thyroid gland, on the development of the thyroid gland explants.

Table 19 shows the amino acid composition and ratio of amino acids included in the peptide complex isolated from the blood vessels.

Table 20 presents the mineral composition of the peptide complex isolated from the blood vessels.

Table 21 presents the content of microelement composition of the peptide complex isolated from the blood vessels.

Table 22 presents the vitamin composition of the peptide complex isolated from the blood vessels.

Table 23 presents the chemical composition of the peptide complex isolated from the blood vessels.

Table 24 shows the effect of peptide complex isolated from vessels, the development of explants vessels.

In Table 25 presents the amino acid composition and ratio of amino acids included in the peptide complex isolated from cartilage.

In Table 26 presents the mineral composition of the peptide complex isolated from cartilage.

In Table 27 presents the elemental composition of the peptide complex isolated from cartilage.

In Table 28 presents the vitamin composition of the peptide complex isolated from cartilage.

Table 29 presents the chemical composition of the peptide complex isolated from cartilage.

Table 30 shows the influence of the tion peptide complex, isolated from cartilage, development of cartilage explants.

Table 31 presents the amino acid composition and ratio of amino acids included in the peptide complex isolated from brain.

Table 32 presents the mineral composition of the peptide complex isolated from brain.

Table 33 presents the elemental composition of the peptide complex isolated from brain.

Table 34 presents the vitamin composition of the peptide complex isolated from brain.

Table 35 presents the chemical composition of the peptide complex isolated from brain.

Table 36 shows the effect of peptide complex isolated from the brain to the development of explants of brain.

Table 37 presents the amino acid composition and ratio of amino acids included in the peptide complex isolated from the thymus.

Table 38 presents the mineral composition of the peptide complex isolated from the thymus.

Table 39 presents the elemental composition of the peptide complex isolated from the thymus.

Table 40 presents the vitamin composition of the peptide complex isolated from the thymus.

Table 41 presents the chemical composition of the peptide complex isolated from the thymus.

Table 42 shows the effect of peptide complex, separation of the aqueous from the thymus, on the development of thymic explants.

Below are examples of obtaining peptide complex from animal products, which use various organs and tissues of animals (example 1 from the liver, example 3 - from the pancreas, an example of 5 - from the thyroid gland, example 7 from vessels, example 9 - of cartilage, example 11 - from the brain, example 13 from the thymus), as well as examples of tissue-specific action peptide complex (example 2 - tissue-specific activity of the peptide complex isolated from liver, example 4 - tissue-specific activity of the peptide complex isolated from pancreas example 6 - tissue-specific activity of the peptide complex isolated from the thyroid gland, example 8 is tissue-specific activity of the peptide complex isolated from vessels, example 10 - tissue-specific activity of the peptide complex isolated from cartilage, example 12 - tissue-specific activity of the peptide complex isolated from brain, example 14 - tissue-specific activity of the peptide complex isolated from thymus).

Example 1. A method of obtaining a peptide complex isolated from liver, with tissue-specific activity

As an animal feedstock use liver of calves (not older than 12 months of age) or pigs.

In R the actor for the extraction pump 250 l of 3% solution of acetic acid and cooled to a temperature of (20± 5)°With, then into the reactor with constant stirring load 50 kg of the crushed material. After 30 minutes add 1% solution of zinc chloride in 3%solution of acetic acid (based on 1 kg of the raw material 10 g of zinc chloride), the suspension with constant stirring is cooled to a temperature plus (7-16)°C. Subsequent mixing for 1 hour every 4 hours of settling. The process is conducted for at least 48 hours at a temperature of plus (7-16)°C.

Separation of the extract from ballast substances is performed on the separator at (5000±500)/min Into the reactor for the deposition of the dipstick acetone serves acetone at a rate of 5 volumes to 1 volume of liver extract. In chilled acetone in a thin stream with constant stirring served clarified liver extract, mix and leave the mixture until the formation of the precipitate at a temperature of plus (3-5)°C for at least 4 hours. The supernatant layer is removed by decantation. The formed precipitate is washed on the suction filter, the mesh of which lay the cotton fabric, two volumes of chilled to plus (7-16)°With acetone, feeding it out of the dipstick. The washed precipitate is forced through a metal sieve with the hole diameter of 1.5 mm and spread a thin layer of enamel pan, cover with a double layer of cotton fabric and dried at t is mperature plus (18± 2)°to remove the smell of acetone. In the drying process a lot as it dries the top layer mix. The resulting powder was sieved through a metal sieve with the hole diameter of 0.36 mm, the Dry powder is weighed, take a sample and send it for analysis.

The yield of the target product (powder biologically active peptide complex isolated from the liver) is 70 g per 1 kg of raw material.

Target product (peptide complex) is a powder from cream to dark brown color and contains biologically active peptide components. The target product is moderately soluble in water.

For more detailed characteristics of the biologically active peptide complex obtained by the proposed method, a study of its composition, the main physico-chemical properties, specific biological activity.

The molecular weight of peptide components in peptide complex, determined by gel-chromatography on Sephadex G-25 and G-50 (Pharmacia, Sweden). For calibration of the column with 1.6×60 cm using a set of markers Peptide Molecuar Weight Kit MS III ("Serva, Germany). It is established that the composition of the peptide complex includes substances with a molecular weight of not more than 12000 Yes. Using reversed-phase high-performance liquid chromatography in a gradient of acetonitril is a (sorbent Lichrosorb C 18", column 2×62 mm) found that the composition of the peptide complex includes; mainly low molecular weight peptide fractions (from 70 to 90%), and high molecular weight components in the peptide complex is missing.

According to electrophoresis in 15%polyacrylamide gel molecular weight of the peptide component of the peptide complex is from 1000 to 12000 Yes.

To identify peptide complex a portion of the target product 10 mg placed in a test tube, dissolve with thorough stirring in 5 ml of water. The solution is filtered through a paper filter. For the preparation of biuret reagent dissolve 90 g of potassium-sodium tartrate in 400 ml of 0.2 n solution of sodium hydroxide, was added 10 g of copper sulfate 5-water, after dissolution, add 10 g of potassium iodide and bring the volume of solution of 0.2 N. sodium hydroxide solution up to 2 l To the investigated solution add 5 ml of biuret reagent. The colour of the solution in the purple color indicates available in the complex of peptide linkages. As a matter of comparison, use water.

Identification of the active peptide complex is carried out using ultraviolet spectrophotometry. For this purpose, 10 mg peptide complex is dissolved in water in a volumetric flask of 100 ml capacity and bring the solution volume to the mark with water. The spectrophotometer measured ultrafire the new range of peptide complex in quartz cuvettes with a 10 mm layer in the region of wavelengths from 250 to 300 nm. As the reference solution using water. Range should have a pronounced maximum at a wavelength (270±5) nm. The ratio of optical density at the wavelength of 275 nm (E275) and 260 nm (D260) D275/D260must be not less than 1.0.

To determine protein peptide complex 25 mg of the product (the exact sample) is placed in a volumetric flask with a capacity of 100 ml, dissolve in water, mixing with a magnetic stirrer, and bring the solution volume to the mark with water. The solution is filtered through a paper filter. Carry out the measurement of the optical density of the samples in quartz cuvettes with a layer thickness of 10 mm at a wavelength of 280 nm (D280) and 260 nm (D260). As the reference solution using water.

The protein content (X) in the product in mg/mg calculated by the formula:

The protein content in the product should be 0.2-0.5 mg/mg.

To determine the residual content of organic solvents (acetone) solution prepared standard sample (RDF) of acetone. About 10 mg (accurately weighed) of acetone (GOST 2603-79, reagent grade.) placed in a volumetric flask with a capacity of 100 ml, the volume was adjusted solution with an aqueous solution of ammonia to the mark and mix. Solution use freshly prepared.

For the determination of acetone to about 100 mg (accurately weighed) of the product is dissolved in 5 ml of p is the target ammonia, the resulting solution was filtered through filter paper. 2 μl of the resulting solution and the solution nor acetone alternately chromatographic gas analyzer ("Chrome 5" or similar) with a flame ionization detector, receiving at least 5 chromatograms.

The acetone (X) in the final product, in percent, is calculated by the formula:

where S1- the average value of the peak areas of the acetone from the chromatograms of the test solution;

S0- the average value of the peak areas of the acetone from the chromatograms of a solution nor acetone;

m1the wt sample of the product in mg;

m0the wt sample RDF acetone, mg;

V1- the volume of the test solution in ml;

V0- the volume of solution nor acetone, Jr.

The results of analysis are considered reliable if the requirements of the test "to test the suitability of the chromatographic system.

Pets the presence of residual amounts of acetone not more than 1%.

Bacteriological indicators peptide complex analyze GOST 10444.2-94, GOST 10444.15-94, GOST R 50474-93, GOST R 50480-93.

The content of toxic elements in the peptide complex is determined according to GOST 26927-86, GOST 26930-86, GOST 26932-86, GOST 26933-86.

The results of the study of the composition of the peptide complex isolated from liver summarized in the following t is the blitz.

The amino acid composition of the peptide complex is determined on the analyzer "LKB-3201 (Sweden). The analysis showed that the peptide complex isolated from the liver, there is a basic range of amino acids, including essential (see Table 1).

Table 1
Name the amino acidsThe content of amino acids, nmol/mg
Asp555
Thr271
Ser306
Glu702
Pro264
Gly385
Ala426
Val312
Ile211
Leu456
Tyr120
Phe194
His138
Lys333
Arg225

The amino acids shown in Table 1, corresponds to the total number of amino acids in peptide complex, equal 65-75 wt.%.

Determination of mineral content in the peptide complex isolated from the liver, carried out by flame photometer (sodium and potassium), tetraedricheskii m is today (calcium and magnesium), calorimetrically (phosphorus, iron). In the process of research revealed that the mineral composition of the peptide complex isolated from liver, presents the most important for the normal functioning of organs and systems of mineral substances in the optimal amount of calcium, magnesium, iron, phosphorus, and potassium and sodium (see Table 2).

Table 2
Name of productThe content of mineral substances, mg/20 mg
CAMgFePToNaS
Peptide complex isolated from liverto 18.011,772,4414,3522,11490,45222,87

The content of mineral substances, presented in Table 2, represents the total amount of mineral substances in the peptide complex is 3.0-5.0 wt.%.

The content of microelements in peptide complex isolated from liver, determine the method of emission spectrometry using spectrochemical system GBC, and reflected in Table 3.

The determination of vitamins in peptide complex isolated from liver, carry out conventional and methods based on the ability of vitamins to give the characteristic color reactions with a number of chemical compounds, the color intensity of which is proportional to the concentration of vitamin a and can be determined fotokolorimetricheskim, and shown in Table 4.

Table 3
Name of productThe content of trace elements, ág/20 mg
AlMnCuCoMo
Peptide complex isolated from liver0,21580,01920,41570,00270,0299

The content of trace elements, are presented in Table 3 corresponds to the total number of trace elements in peptide complex, equal 0,003-0,008 wt.%.

Table 4
Name

product
The content of vitamins, mg/20 mg
In1In2RRAndE
Peptide complex isolated from liver0,080,0593,012traces0,0190040

The content of vitamins, presented in Table 4, represents the total amount of vitamins in the peptide complex of 0.01-0.03 wt.%.

The analysis of the chemical composition of the target product indicates the absence of carbohydrates and low energy value that can be used in diet foods (see Table 5).

Table 5
Name

product
Energy

value cal/20 mg
Component content, mg/20 mg
ProteinsFatsCarbohydratesAsh
Peptide complex isolated from liver42,158,120,92traces0,28

Contains information on the chemical composition of the product obtained by the proposed method, indicating the presence of physiological concentrations of amino acids, minerals, trace elements and vitamins, shows the chemical composition of the tissue of which was carried out extraction, and confirms the content of these substances into biologically bound form.

Example 2. Tissue-specific activity of the peptide complex, vyd is certain from the liver

To study the tissue-specific activity of the peptide complex, selected by the proposed method from the liver, we studied the effect of the target product on the growth of the organotypic culture of the liver of adult rats "Wistar" weighing 150-200 g

Prepared under sterile conditions, the fragments of the rat liver was divided into smaller sized pieces about 1 mm3that were placed in Petri dishes with collagen coating the bottom. Culture medium consisted of 35% eagle medium, 35% Hanks solution, 25% fetal calf serum, 5% chicken embryo extract, 0.6% glucose, 0.5 u/ml insulin, 100 units/ml of gentamicin. The investigated peptide complex isolated from liver, was introduced into the culture medium in concentrations of from 0.01 to 20 ng/ml to identify its effective concentration.

In a Petri dish filled with experimental explants was added to 3 ml of culture medium containing peptide complex isolated from the liver, in the studied concentration, and in Petri dishes with control explants of 3 ml of culture medium; thus, the experimental and the control explants was developed in the same volume of nutrient medium. Petri dishes were placed in a thermostat at a temperature of (37±0,5)°and after 3 days were viewed under phase-contrast microscope. Defined area index (PI), which RA is read in arbitrary units as the ratio of total Explant with zone visulaise cells to the Central area of the Explant.

For visualization of explants used mikrotalasna microscope (series 10, MTN-13 "alpha Telecom", Russia). To calculate the index area of the explants used the Photo M 1.2. The significance of differences in the index area of the control and experimental explants was evaluated using t-student criterion. Values area index expressed in percent of the control value IC has been taken for 100%.

When using peptide complex isolated from the liver at concentrations of 0.05, 0.1 and 10 ng/ml, there was a significant increase in PI explants on 18-28% compared with control values PI. The obtained data are presented in Table 6.

Table 6
IndexThe concentration of the peptide complex isolated from liver, ng/ml
0,010,050,10,51,02,010,020,0
PI % relative to the control518*20*22*1422*28*18*
* p<0.05 compared to control.

Thus, in relation to liver tissue peptide idea is to, isolated from the liver, has a tissue-specific effect, resulting in stimulation of growth of the explants, which is confirmed in experiments on rats, it can be considered demonstrates use it as a tool that supports liver function.

Example 3. A method of obtaining a peptide complex isolated from the pancreas, with tissue-specific activity

As an animal feedstock used the pancreas of calves (not older than 12 months of age) or pigs.

Selection of peptide complex from the pancreas were carried out as described in example 1, except that the yield of the target product (powder biologically active peptide complex isolated from the pancreas) is 60 g per 1 kg of raw material.

For more detailed characteristics of the biologically active peptide complex obtained by the proposed method, a study of its composition, the main physico-chemical properties, specific biological activity.

The main characteristics of the peptide complex isolated from the pancreas, certain methods described in example 1 are presented in Tables 7-11.

Amino acid analysis showed that the peptide complex isolated from the pancreas, is the main range of AMI is ocelot, including irreplaceable (see Table 7).

The mineral composition of the peptide complex isolated from the pancreas, presents the most important for the normal functioning of organs and systems of mineral substances in the optimal amount of calcium, magnesium, iron, phosphorus, and potassium and sodium, which are shown in Table 8.

The results of the analysis of the elemental composition of the peptide complex, selected by the proposed method from the pancreas, are presented in Table 9.

Vitamin composition of the peptide complex, selected by the proposed method from the pancreas, characterized by a significant content of vitamin PP,2and the presence of vitamin B1, A and E, as shown in Table 10.

The analysis of the chemical composition of the target product indicates the absence of carbohydrates and low energy value that can be used in diet foods (see Table 11).

Table 7
Name the amino acidsThe content of amino acids, nmol/mg
Asp388
Thr171
Ser212
Glu505
Pro 15)
Gly401
Ala314
Val305
Ile144
Leu352
Tyr81
Phe111
His129
Lys306
Arg187

The amino acids shown in Table 7, corresponding to total number of amino acids in peptide complex, equal 45-55 wt.%.

Table 8
Name

product
The content of mineral substances, mg/20 mg
CAMgFePToNaS
Peptide complex isolated from the pancreas30,142,001,0176,14100,541921,41306,17

The content of mineral substances, presented in Table 8 corresponds to the total amount of mineral substances in peptide complex, equal to 10.0 to 15 wt.%.

Table 9
Name of productThe content of trace elements, ág/20 mg
AlMnCuCoMo
Peptide complex isolated from the pancreas0,32110,05120,071200,0190

The content of trace elements, are presented in Table 9, corresponding to total number of trace elements in peptide complex, equal to 0,001-0,003 wt.%.

Table 10
Name

product
The content of vitamins, mg/20 mg
B1In2RRAndE
Peptide complex isolated from the pancreas0,090,2121,255traces0,0110,221

The content of vitamins, presented in Table 10 corresponds to the total amount of vitamins in peptide complex, equal to 0,005-0,010 wt.%.

Table 11
Name

product
Energy

value cal/20 mg
Component content, mg/20 mg
ProteinsFatsCarbohydratesAsh
Peptide complex isolated from the pancreas41,126,051,47traces1,0

Contains information on the chemical composition of the product obtained by the proposed method, indicating the presence of physiological concentrations of amino acids, minerals, trace elements and vitamins, shows the chemical composition of the tissue of which was carried out extraction, and confirms the content of these substances into biologically bound form.

Example 4. Tissue-specific activity of the peptide complex isolated from the pancreas

To study the tissue-specific activity of the peptide complex isolated from the pancreas by the proposed method, investigated its impact on growth in organotypic cultures of the pancreas of adult rats. In detail the method described in example 2.

The experiments were carried out on 29 fragments of the pancreas of rats "Wistar" weighing 150-200 g Fragments of the pancreas was placed in a nutrient medium and cultured in Petri dishes in which the C thermostat at (37± 0,5)°C for 2 days. In the experimental medium was added peptide complex isolated from the pancreas, in concentrations of from 0.01 to 20 ng/ml.

When using peptide complex isolated from the pancreas in concentrations of 0.05, 0.1 and 10 ng/ml, there was a significant increase in PI explants on 16-27% compared with control values PI. The obtained data are presented in Table 12.

Table 12
IndexThe concentration of the peptide complex isolated from the pancreas, ng/ml
0,010,050,10,51,02,010,020,0
PI % relative to the control820*27*20*16*22*24*16*
• p<0.05 compared to control.

Thus, in respect of pancreatic tissue peptide complex isolated from the pancreas, has a tissue-specific effect, resulting in stimulation of growth of the explants, which is confirmed in experiments on rats, it allows us to consider shows the use of the Finance it as a means, supporting the function of the pancreas.

Example 5. A method of obtaining a peptide complex isolated from the thyroid gland, with tissue-specific activity

As an animal feedstock use the thyroid gland of calves (not older than 12 months of age) or pigs.

The selection of the peptide complex of the thyroid gland were carried out as described in example 1, except that the yield of the target product (powder biologically active peptide complex isolated from the thyroid gland) is 46 g per 1 kg of raw material.

For more detailed characteristics of the biologically active peptide complex isolated from the thyroid gland obtained by the proposed method, a study of its composition, the main physico-chemical properties, specific biological activity.

Main characteristics of the obtained peptide complex isolated from the thyroid gland, certain methods described in example 1 are presented in Tables 13-17.

Amino acid analysis of the target product showed that peptide complex isolated from the thyroid gland, there is a basic range of amino acids, including essential (see Table 13).

Table 13
Called the e amino acids The content of amino acids, nmol/mg
Asp502
Thr241
Ser405
Glu891
Pro377
Gly505
Ala594
Val418
Ile158
Leu588
Tyr126
Phe251
His131
Lys305
Arg201

The amino acids shown in Table 13, corresponds to the total number of amino acids in peptide complex, equal to 70-80 wt.%.

The mineral composition of the peptide complex isolated from the thyroid gland, presents the most important for the normal functioning of organs and systems of mineral substances in the optimal amount of calcium, magnesium, iron, phosphorus, and potassium and sodium, as shown in Table 14.

The results of the analysis of the elemental composition of the peptide complex isolated from the thyroid gland, are presented in Table 15.

Vitamin composition of the peptide complex isolated from the thyroid gland, characterized by C is acitelli vitamin PP, In2and the presence of vitamins In1, A and E, as shown in Table 16.

The analysis of the chemical composition of the target product indicates the absence of carbohydrates and low energy value that can be used in diet foods (see Table 17).

Table 14
Name

product
The content of mineral substances, mg/20 mg
CAMgFePToNaS
Peptide complex isolated from the thyroid gland20,141,02a 3.8742,0740,11261,12277,18

The content of mineral substances, presented in Table 14 represents the total amount of mineral substances in the peptide complex is 3.0-5.0 wt.%.

Table 15
Name

product
The content of trace elements, ág/20 mg
AlMnCuCoMo
Peptide complex isolated from shields the ne gland 0,50800,04200,083500,0102

The content of trace elements, are presented in Table 15, corresponds to the total number of trace elements in peptide complex 0.002-0.004 wt.%.

Table 16
Name

product
The content of vitamins, mg/20 mg
B1In2RRAndE
Peptide complex isolated from the thyroid gland0,190,0200,035traces0,0290,057

The content of vitamins presented in Table 16 represents the total amount of vitamins in peptide complex, equal to 0,001-0,003 wt.%.

Table 17
Name

product
Energy

value, cal/20 mg
Component content, mg/20 mg
ProteinsFatsCarbohydratesAsh
Peptide complex isolated from sitewideusers 44,847,011,33traces0,18

Contains information on the chemical composition of the product obtained by the proposed method, indicating the presence of physiological concentrations of amino acids, minerals, trace elements and vitamins, shows the chemical composition of the tissue of which was carried out extraction, and confirms the content of these substances into biologically bound form.

Example 6. Tissue-specific activity of the peptide complex isolated from the thyroid gland

To study the tissue-specific activity of the peptide complex, selected by the proposed method from the thyroid gland, investigated the influence of the target product on the growth of the organotypic culture of the thyroid gland of Mature rats. In detail the method described in example 2.

The experiments were carried out on 35 fragments of the thyroid gland of rats "Wistar" weighing 150-200 g Fragments of the thyroid gland was placed in a nutrient medium and cultured in Petri dishes in an incubator at 37±0,5)°C for 2 days. In the experimental medium was added peptide complex isolated from the thyroid gland, in concentrations of from 0.01 to 20 ng/ml.

When using peptide complex isolated from the thyroid gland in concentric the s 0,05, 0.1 and 10 ng/ml, there was a significant increase in PI explants at 18-24% compared with the control values of PI.

Table 18 shows the effect of peptide complex isolated from the thyroid gland, on the development of the thyroid gland explants.

Table 18
IndexThe concentration of the peptide complex isolated from the thyroid gland, ng/ml
0,010,050,10,51,02,010,020,0
PI % relative to the control718*20*20*1520*24*20*
• p<0.05 compared to control.

Thus, in relation to the tissue of the thyroid gland peptide complex isolated from the thyroid gland, has a tissue-specific effect, resulting in stimulation of growth of the explants, which is confirmed in experiments on rats, it can be considered demonstrates use it as a tool that supports thyroid function.

Example 7. A method of obtaining a peptide complex isolated from vessels with Danes ecifically activity

As an animal feedstock use vessels calves (not older than 12 months of age) or pigs.

The selection of the peptide complex of the vessels were carried out as described in example 1, except that the yield of the target product (powder biologically active peptide complex isolated from blood vessels) is 28 g per 1 kg of raw material.

For more detailed specifications peptide complex obtained by the proposed method, a study of its composition, the main physico-chemical properties, specific biological activity.

Main characteristics of the obtained peptide complex isolated from vessels, certain methods described in example 1 are presented in Tables 19-23. Amino acid analysis of the target product showed that peptide complex isolated from vessels, there is a basic range of amino acids, including essential (see Table 19).

Table 19
Name the amino acidsThe content of amino acids, nmol/mg
Asp755
Thr361
Ser402
Glu999
Pro374
Gly452
Ala502
Val401
Ile247
Leu504
Tyr200
Phe238
His231
Lys498
Arg356

The amino acids shown in Table 19, corresponds to the total number of amino acids in peptide complex, equal 80,0 to 90.0 wt.%.

The mineral composition of the peptide complex isolated from vessels, presents the most important for the normal functioning of organs and systems of mineral substances in the optimal amount of calcium, magnesium, iron, phosphorus, and potassium and sodium, as shown in Table 20.

Table 20
Name

product
The content of mineral substances, mg/20 mg
CAMgFePToNaS
Peptide complex isolated from vessels25,743,031,7125,1734,55222,51444,45

Sod is neigh mineral substances, presented in Table 20 represents the total amount of mineral substances in the peptide complex is 3.0-5.0 wt.%. The results of the analysis of the elemental composition of the peptide complex isolated from vessels is presented in Table 21.

Table 21
Name

product
The content of trace elements, ág/20 mg
AlMnCuCoMo
Peptide complex isolated from vessels0,98120,01230,584600,0110

The content of trace elements, are presented in Table 21 corresponds to the total number of trace elements in peptide complex, equal to 0,005-0,010%by weight. Vitamin composition of the peptide complex isolated from blood vessels, characterized by a significant content of vitamin PP,2and the presence of vitamin B1, A and E, which are presented in Table 22.

Table 22
Name

product
The content of vitamins, mg/20 mg
In1In2P is AndE
Peptide complex isolated from vessels0,330,0020,041traces0,0230,039

The content of vitamins, presented in Table 22, represents the total amount of vitamins in peptide complex, equal to 0,001 - 0,003 wt.%.

The analysis of the chemical composition of the target product indicates the absence of carbohydrates and low energy value that can be used in diet foods (see Table 23).

Table 23
Name

medication
EnergyComponent content, mg/20 mg
value cal/20 mgProteinsFatsCarbohydratesAsh
Peptide complex isolated from vessels39,157.850,86traces0,35

Contains information on the chemical composition of the product obtained by the proposed method, indicating the presence of physiological concentrations of amino acids, minerals, trace elements and vitamins, shows him the ical composition of the tissue, of which was carried out extraction, and confirms the content of these substances into biologically bound form.

Example 8. Tissue-specific activity of the peptide complex isolated from vessels

To study the tissue-specific activity of the peptide complex isolated from the blood vessels of the proposed method, we studied the effect of the target product on the growth of tissue culture vessels Mature rats. In detail the method described in example 2.

The experiments were carried out on the 24 fragments of tissue vessels of rats "Wistar" weighing 150-200 g Fragments of tissue vessels were placed in a nutrient medium and cultured in Petri dishes in an incubator at 37±0,5)°C for 2 days. In the experimental medium was added peptide complex isolated from vessels, in concentrations of from 0.01 to 20 ng/ml.

When using peptide complex isolated from vessels at concentrations of 0.05, 0.1 and 10 ng/ml was observed a significant increase of FE explants at 18-25% compared with the control values of PI.

Table 24 shows the effect of peptide complex isolated from vessels, the development of explants vessels.

Table 24
IndexThe concentration of the peptide complex is, selected from vessels, ng/ml
0,010,050.10,51,02,010,020,0
PI % relative to the control722*24*161418*25*14
• p<0.05 compared to control.

Thus, in relation to tissue vessels peptide complex isolated from vessels, has a tissue-specific effect, resulting in stimulation of growth of the explants, which is confirmed in experiments on rats, it can be considered demonstrates use it as a tool that supports the function of blood vessels.

Example 9. A method of obtaining a peptide complex isolated from cartilage with tissue-specific activity

As an animal feedstock using cartilage calves (not older than 12 months of age) or pigs.

The selection of the peptide complex of cartilage were carried out as described in example 1, except that the yield of the final product (powder biologically active peptide complex isolated from cartilage) is 30 g per 1 kg of raw material.

For more detailed characteristics of the biologically active peptide complex obtained from HRA what she offered by way a study of its composition, the main physico-chemical properties, specific biological activity.

Main characteristics of the obtained peptide complex isolated from cartilage, certain methods described in example 1 are presented in Tables 25-29.

Amino acid analysis of the target product showed that peptide complex isolated from cartilage, there is a basic range of amino acids, including essential (see Table 25).

Table 25
Name the amino acidsThe content of amino acids, nmol/mg
Asp481
Thr198
Ser244
Glu602
Pro201
Gly416
Ala402
Val233
Ile151
Leu375
Tyr111
Phe154
His120
Lys305
Arg198

The amino acids shown in Table 25, corresponds to the total number of amine the acids in peptide complex, equal to 50-60 wt.%.

The mineral composition of the peptide complex isolated from cartilage, presents the most important for the normal functioning of organs and systems of mineral substances in the optimal amount of calcium, magnesium, iron, phosphorus, and potassium and sodium, as shown in Table 26.

Table 26
Name

product
The content of mineral substances, mg/20 mg
CAMgFePToNaS
Peptide complex isolated from cartilage21,172,051,3328,8990,361222,44270,62

The content of mineral substances, presented in Table 26, represents the total amount of mineral substances in peptide complex, equal to 7.0, and 9.0 wt.%.

The results of the analysis of the elemental composition of the peptide complex isolated from cartilage, are presented in Table 27.

Vitamin composition of the peptide complex isolated from cartilage, characterized by a significant content of vitamin PP,2and the presence of vitamin B1, A and E, which are presented in Tab is itzá 28.

Table 27
Name of productThe content of trace elements, ág/20 mg
AlMnCuCoMo
Peptide complex isolated from cartilage0,59260,01980,07020,00100,0111

The content of trace elements, are presented in Table 27, corresponds to the total number of trace elements in peptide complex, equal to 0,002-0,008 wt.%.

Table 28
Name

product
The content of vitamins, mg/20 mg
B1In2RRAndE
Peptide complex isolated from cartilage0,340,0070,014traces0,0200,040

The content of vitamins, presented in Table 28, corresponds to the total amount of vitamins in peptide complex, equal to 0,001-0,003 wt.%.

The analysis of the chemical composition of the target product indicated the absence in what you eat carbohydrates and low energy value, it can be used in diet foods (see Table 29).

Table 29
Name

product
EnergyComponent content, mg/20 mg
value cal/20 mgProteinsFatsCarbohydratesAsh
Peptide complex isolated from cartilage42,636,941,54traces0,48

Contains information on the chemical composition of the product obtained by the proposed method, indicating the presence of physiological concentrations of amino acids, minerals, trace elements and vitamins, shows the chemical composition of the tissue of which was carried out extraction, and confirms the content of these substances into biologically bound form.

Example 10. Tissue-specific activity of the peptide complex isolated from cartilage

To study the tissue-specific activity of the peptide complex, highlighted the proposed method of cartilage, investigated the influence of the target product on the growth of the organotypic culture of the cartilage tissue of adult rats. In detail the method described in example 2.

The former is eriment held on 30 fragments of the cartilage of rats "Wistar" weighing 150-200 g Fragments of cartilage tissue was placed in a nutrient medium and cultured in Petri dishes in an incubator at 37±0,5)°C for 2 days. In the experimental medium was added peptide complex isolated from cartilage, in concentrations of from 0.01 to 20 ng/ml.

When using peptide complex isolated from cartilage at concentrations of 0.05, 0.1 and 10 ng/ml was observed a significant increase of FE explants on 19-25% compared with the control values of PI.

Table 30 shows the effect of peptide complex isolated from cartilage, development of explants of cartilage.

Table 30
IndexThe concentration of the peptide complex of cartilage, ng/ml
0,010,050,10,51,02,010,020,0
PI % relative to the control520*22*19*1620*25*12
• p<0.05 compared to control.

Thus, in respect of cartilage peptide complex isolated from cartilage, has a tissue-specific effect, resulting in stimulation of the OST explants, confirmed in experiments on rats, it can be considered shown using it as a tool that supports cartilage.

Example 11. A method of obtaining a peptide complex isolated from the brain with tissue-specific activity

As an animal feedstock use the brain calves (not older than 12 months of age) or pigs.

The selection of the peptide complex of the brain were carried out as described in example 1, except that the yield of the final product (powder biologically active peptide complex isolated from the brain) is 44 g per 1 kg of raw material.

For more detailed characteristics of the biologically active peptide complex obtained from the brain of the proposed method, a study of its composition, the main physico-chemical properties, specific biological activity.

Main characteristics of the obtained peptide complex isolated from brain, certain methods described in example 1 are presented in Tables 31-35.

Amino acid analysis of the target product showed that peptide complex isolated from brain, there is a basic range of amino acids, including essential (see Table 31).

Table 31
Name the amino acidsThe content of amino acids, nmol/mg/
Asp491
Thr198
Ser285
Glu602
Pro191
Gly305
Ala355
Val233
Ile164
Leu384
Tyr101
Phe177
His122
Lys255
Arg197

The amino acids shown in Table 31, corresponds to the total number of amino acids in peptide complex, equal to 50-60 wt.%.

The mineral composition of the peptide complex isolated from brain, presents the most important for the normal functioning of organs and systems of mineral substances in the optimal amount of calcium, magnesium, iron, phosphorus, and potassium and sodium, as shown in Table 32.

Table 32
Name

product
The content of mineral substances, mg/20 mg
CAMgFePToNaS
Peptide complex isolated from brain36,112,881,0050,4421,18674,25147,30

The content of mineral substances, are presented in Table 32, corresponds to the total amount of mineral substances in the peptide complex is 4.0 to 6.0 wt.%. The results of the analysis of the elemental composition of the peptide complex isolated from brain, are presented in Table 33.

Table 33
Name

product
The content of trace elements, ág/20 mg
AlMnCuCoMo
Peptide complex isolated from brain0,28440,01310,19980,00280,0190

The content of trace elements, are presented in Table 33, corresponds to the total number of trace elements in peptide complex 0.002-0.004 wt.%.

Vitamin composition of the peptide complex, vydeleny the century of the brain, characterized by a significant content of vitamin PP,2and the presence of vitamin B1, A and E, which are presented in Table 34.

Table 34
Name

product
The content of vitamins, mg/20 mg
B1In2RRCAndE
Peptide complex isolated from brain0,070,0270,063traces0,0100,005

The content of vitamins, presented in Table 34, corresponds to the total amount of vitamins in peptide complex, equal to 0,001-0,003 wt.%.

The analysis of the chemical composition of the target product indicates the absence of carbohydrates and low energy value that can be used in diet foods (see Table 35).

Table 35
Name

medication
EnergyComponent content, mg/20 mg
value cal/20 mgProteinsFatsCarbohydrates Ash
Peptide complex isolated from brain48,136.42 per1,98traces0,41

Contains information on the chemical composition of the product obtained by the proposed method, indicating the presence of physiological concentrations of amino acids, minerals, trace elements and vitamins, shows the chemical composition of the tissue of which was carried out extraction, and confirms the content of these substances into biologically bound form.

Example 12. Tissue-specific activity of the peptide complex isolated from brain

To study the tissue-specific activity of the peptide complex, selected by the proposed method from the brain, we studied the effect of the target product on the growth of organotypic culture brain of adult rats. In detail the method described in example 2.

Experiments conducted on 32 slices of rat brain line "Wistar" weighing 150-200 g Slices of rat brain was placed in a nutrient medium and cultured in Petri dishes in an incubator at 37±0,5)°C for 2 days. In the experimental medium was added peptide complex isolated from brain, in concentrations of from 0.01 to 20 ng/ml.

When ispolzovaniya complex, isolated from the brain at concentrations of 0.05, 0.1 and 10 ng/ml, there was a significant increase in PI explants on 20-28% compared with the control values of PI.

Table 36 shows the effect of peptide complex isolated from the brain to the development of explants of brain.

Table 36
IndexThe concentration of the peptide complex isolated from brain, ng/ml
0,010,050,10,51,02,010,020,0
PI % relative to the control824*24*24*20*26*28*20*
• p<0.05 compared to control.

Thus, in relation to brain tissue peptide complex isolated from brain, has a tissue-specific effect, resulting in stimulation of growth of the explants, which is confirmed in experiments on rats, it can be considered shown using it as a tool that supports brain.

Example 13. A method of obtaining a peptide complex, vyd is certain from the thymus, with tissue-specific activity

As an animal feedstock use the thymus (thymus) calves (not older than 12 months of age) or pigs.

The selection of the peptide complex of the thymus were carried out as described in example 1, except that the yield of the final product (powder biologically active peptide complex isolated from thymus) is 54 g per 1 kg of raw material.

For more detailed characteristics of the biologically active peptide complex obtained by the proposed method, a study of its composition, the main physico-chemical properties, specific biological activity.

Main characteristics of the obtained peptide complex isolated from thymus, certain methods described in example 1 are presented in Tables 37-41.

Amino acid analysis of the target product showed that peptide complex isolated from thymus, there is a basic range of amino acids, including essential (see Table 37).

The mineral composition of the peptide complex isolated from thymus, presents the most important for the normal functioning of organs and systems of mineral substances in the optimal amount of calcium, magnesium, iron, phosphorus, and potassium and sodium, which are presented in Table 38.

The results of anal is for microelement composition of the peptide complex, isolated from thymus, are presented in Table 39.

Vitamin composition of the peptide complex isolated from thymus, characterized by a high content of vitamins PP,1as well as the presence of vitamin B2, A and E (see Table 40).

Table 37
Name the amino acidsThe content of amino acids, nmol/mg
Asp398
Thr241
Ser288
Glu605
Pro233
Gly477
Ala451
Val245
Ile182
Leu341
Tyr100
Phe119
His132
Lys422
Arg356

The amino acids shown in Table 37, corresponding to total number of amino acids in peptide complex, equal 55-65 wt.%.

Table 38
Name

product
The content is the use of mineral substances, mg/20 mg
CAMgFePToNaS
Peptide complex isolated from thymus45,194,014,2271,6121,84465,11133,48

The content of mineral substances, are presented in Table 38, corresponds to the total amount of mineral substances in the peptide complex is 3.0-5.0 wt.%.

Table 39
Name

product
The content of trace elements, ág/20 mg
AlMnCuCoMo
Peptide complex isolated from thymus0,24590,00740,03990,00300,0036

The content of trace elements, are presented in Table 39, corresponds to the total number of trace elements in peptide complex, equal to 0,001-0,003 wt.%.

Table 40
Name

product
The content of vitamins, mg/20 mg
B1 In2RRAndE
Peptide complex isolated from thymus0,090,0071,788traces0,0221,000

The content of vitamins, are presented in Table 40 corresponds to the total amount of vitamins in peptide complex, equal 0,010-0,030 wt.%.

The analysis of the chemical composition of the target product indicates the absence of carbohydrates and low energy value that can be used in diet foods (see Table 41).

Table 41
Name

product
EnergyComponent content, mg/20 mg
value cal/20 mgProteinsFatsCarbohydratesAsh
Peptide complex isolated from thymus39,656,441,22traces1,02

Contains information on the chemical composition of the product obtained by the proposed method, indicating the presence of physiological concentrations of amino acids, minerals, trace elements and Vita is ins shows the chemical composition of the tissue of which was carried out extraction, and confirms the content of these substances into biologically bound form.

Example 14. Tissue-specific activity of the peptide complex isolated from thymus

To study the tissue-specific activity of the peptide complex, selected by the proposed method from the thymus, investigated the influence of the target product on the growth of organotypic cultures of thymic Mature rats. In detail the method described in example 2.

Experiments conducted on 32 fragments of the thymus of rats "Wistar" weighing 150-200 g Fragments goitrous glands were placed in a nutrient medium and cultured in Petri dishes in an incubator at 37±0,5)°C for 2 days. In the experimental medium was added peptide complex isolated from thymus, in concentrations of from 0.01 to 20 ng/ml.

When using peptide complex isolated from thymus, in concentrations of 0.05, 0.1 and 10 ng/ml was observed a significant increase of FE explants by 20-30%, compared with the control values of PI.

Table 42 shows the effect of peptide complex isolated from thymus, the development of thymic explants.

Table 42
IndexConc is tion peptide complex thymus, ng/ml
0,010,050,10,51,02,010,020,0
PI % relative to the control726*26*27*20*27*30*16
• p<0.05 compared to control.

Thus, in relation to the tissues of the thymus peptide complex isolated from thymus, has a tissue-specific effect, resulting in stimulation of growth of the explants, which is confirmed in experiments on rats, it can be considered demonstrates use it as a means for correction of immune system functions.

The examples above clearly confirm that the obtained target product is a peptide complex with a molecular mass of its constituent peptide components from 1000 to 12000 Yeah, containing biologically bound form amino acids, trace elements, minerals and vitamins that are specific to organs and tissues from which it is selected.

1. The method of obtaining funds for maintenance therapy with tissue-specific activity, characterized by the fact that the bodies of calves under 12 months of age or pig chop, add 3%Rast is the PR of acetic acid at 20± 5°With, the extraction was carried out with constant stirring, over 30 min add 1%solution of zinc chloride, cooled with constant stirring to 7-16°C, then stirred for 1 h every 4 h settling for 48 h, the extract is separated from the ballast substances separating, to the extract add acetone in a volume ratio of 1:5, is maintained at 3-5°C for 4 h, the precipitate was washed with two volumes of chilled until 7-16°With acetone, washed precipitate wipe through a metal sieve, obtained target product, representing peptide complex containing low molecular weight peptide fractions of from 70 to 90%, dried at 18±2°C.

2. The method according to claim 1, characterized in that the maximum absorption of the target product in the ultraviolet spectrum is 270±5 nm.

3. Means for supporting therapy with tissue-specific activity, characterized in that it is obtained by the method according to claim 1 of the liver tissue and represents a peptide complex, supports liver function, with a molecular mass of its constituent peptide components in the range from 1000 to 12000 Yes, which contains amino acids, minerals, trace elements and vitamins in biologically bound form in the following components:

Amino acids120-702 nmol/mg
Minerals1,77-490,45 mcg/20 mg
Trace elements0,0027-0,4157 mcg/20 mg
Vitamins0,019-3,012 mcg/20 mg

4. Means for supporting therapy with tissue-specific activity, characterized in that it is obtained by the method according to claim 1 of the tissues of the pancreas and is a peptide complex with a molecular mass of its constituent peptide components in the range from 1000 to 12000 Yes, supporting the function of the pancreas, contains amino acids, minerals, trace elements and vitamins in biologically bound form in the following components:

Amino acids81-505 nmol/mg
Minerals1,01-1921,41 mcg/20 mg
Trace elements0,0190-0,3211 mcg/20 mg
Vitamins0,011-1,255 mg/20 mg

5. Means for supporting therapy with tissue-specific activity, characterized in that it is obtained by the method according to claim 1 of the thyroid tissue and represents a peptide complex with a molecular mass of its constituent peptide components is tov in the range from 1000 to 12000 Yes, supporting the function of the thyroid gland, contains amino acids, minerals, trace elements and vitamins in biologically bound form in the following components:

Amino acids126-891 nmol/mg
Minerals1,02-277,18 mcg/20 mg
Trace elements0,0102-0,5080 mcg/20 mg
Vitaminsof 0.02 to 0.19 nmol/mg

6. Means for supporting therapy with tissue-specific activity, characterized in that it is obtained by the method according to claim 1 of vessels and represents a peptide complex with a molecular mass of its constituent peptide components in the range from 1000 to 12000 Yes, support vessels, contains amino acids, minerals and vitamins in biologically bound form in the following components:

Amino acids200-999 nmol/mg
Minerals1,71-444,45 mcg/20 mg
Trace elements0,011-0,9812 mcg/20 mg
Vitamins0,002-0,33 mg/20 mg

7. Means for supporting therapy with tissue-specific activity, harakteryzuyetsya fact, it is obtained by the method according to claim 1 of the cartilage and is a peptide complex with a molecular mass of its constituent peptide components in the range from 1000 to 12000 Yes, supports cartilage, contains amino acids, minerals, trace elements and vitamins in biologically bound form in the following components:

Amino acids111-602 nmol/mg
Minerals1,33-1222,44 mcg/20 mg
Trace elements0,0010-0,5926 mcg/20 mg
Vitamins0,007-0,34 mg/20 mg

8. Means for supporting therapy with tissue-specific activity, characterized in that it is obtained by the method according to claim 1 from brain tissue and is a peptide complex with a molecular mass of its constituent peptide components in the range from 1000 to 12000 Yes, supports brain, contains amino acids, minerals, trace elements and vitamins in biologically bound form in the following components:

Amino acids101-602 nmol/mg
Minerals1,0-674,25 mcg/20 mg
Trace elements0,0028-0,2844 mcg/20 mg
Vitaminsfrom 0.005 to 0.07 mg/20 mg

9. Means for supporting therapy with tissue-specific activity, characterized in that it is obtained by the method according to claim 1 of the thymus and is a peptide complex c molecular weight constituent peptide components in the range from 1000 to 12000 Yes, supports immune system, contains amino acids, minerals, trace elements and vitamins in biologically bound form in the following components:

Amino acids100-605 nmol/mg
Minerals4,01-465,11 mcg/20 mg
Trace elements0,0030-0,2459 mcg/20 mg
Vitamins0,007-1,788 mcg/20 mg



 

Same patents:

FIELD: biotechnology, virology, medicine.

SUBSTANCE: invention relates to attenuated virus derived from modified Ankara vaccina virus. Said virus are not able for reproduction by replication in human cell lines. Also disclosed are application of virus or recombinant variants thereof as drug or vaccine, as well as method for inducing of immune response in patients with defected immunity, in patients having immunity to vaccine virus, or in patient during antiviral therapy.

EFFECT: variant of Ankara vaccina virus effective in medicine and veterinary.

86 cl, 15 dwg, 1 tbl, 2 ex

FIELD: medicine, in particular, oncology and urology, possible use for treatment of surface cancer of urinary bladder.

SUBSTANCE: in accordance to method, tumor is irradiated by laser with output power 0,5-2 Wt with wave length 662 nm and light energy of 300-600 J/cm2 during 10-30 minutes in presence of specified photosensitizer injected intravenously in volume of 0,8-1 mg/kg. Then a silicon vessel is inserted into urinary bladder with fibro-optical filament with cylindrical diffuser positioned therein. Vessel is filled with distilled water and laser irradiation of whole mucous tunic of urinary bladder by light energy at 30-40 J/cm2 is continued during 40-60 minutes.

EFFECT: possible decrease of frequency of relapses and collateral reactions.

1 ex

FIELD: pharmacy, chemical technology, medicine.

SUBSTANCE: invention proposes a composition that comprises 6-decaprenyl-2,3-dimethoxy-5-methyl-1,4-benzoquinone as an active component, antioxidant, non-ionogenic surface-active substance, preserving agent, lipid-soluble stabilizing agent of emulsion, water-soluble stabilizing of emulsion and water. Method for preparing the indicated composition involves mixing the definite amounts of non-ionogenic surface-active substance with antioxidant, heating to temperature 40-120°C, dissolving the necessary amounts of lipid-soluble stabilizing agent of emulsion and 6-decaprenyl-2,3-dimethoxy-5-methyl-1,4-benzoquinone are dissolved in the prepared solution. The prepared mixture is added at intensive stirring to the mixed solution of water-soluble stabilizing agent of emulsion and preserving agent in water preliminary heated to temperature 30-100°C. Invention provides improving bioavailability and increasing storage time of the composition. Proposed composition is used in prophylaxis and treatment of different diseases and for recreating the working ability.

EFFECT: improved preparing method, valuable medicinal properties of composition.

2 cl, 1 tbl, 5 ex

FIELD: medicine, traumatology, resuscitation, field surgery, medicine of catastrophes.

SUBSTANCE: the present innovation deals with helping patients at any variant of limb's detachment at its preparing to replantation. Into soft tissues of detached limb one should introduce oxygenated perfluorane at the dosage of 30 mg/kg of detached limb, and then it should be placed into replantation sac. The innovation enables to hold back the development of ischemic toxicosis due to creating the stock of oxygen carrier, temporarily, up to the moment of replanting, improve tissue structures, up to the moment of replantation, improve the safety of tissue structures and increase the quality of replant's functioning.

EFFECT: higher efficiency.

1 ex

FIELD: medicine, cosmetology.

SUBSTANCE: claimed method includes administration of dispersed biological material diluted in physiological solution or topical anesthetic agent solution in amount of 10-50 mg biological material per 1-15 ml of solution, in dermal and subdermal skin layers. Solution is injected in single dose of 0.1-5 ml and number of injections is 1-20 per course and number of courses is 1-5 with distance between courses from 2 days to 3-4 weeks.

EFFECT: method with increased effectiveness due to activation of base structures of dermal matrix.

2 ex

FIELD: medicine, cosmetology.

SUBSTANCE: claimed method includes application of mask based on natural ion exchanger minerals or sorbents with addition of sterile fly larva ground to paste-like state in ratio of 1:2.5, wherein mineral part size is not more than 1 mm. Before application composition is agitated up to full paste absorption and dries for 30-40 min followed by application on skin surface.

EFFECT: mask with prolonged hemolymph storage time and increased effectiveness.

1 dwg, 4 cl

FIELD: medicine, pharmacy.

SUBSTANCE: invention relates to a method for treatment of vascular proliferation in a patient. Method involves administration of agonist of somatostatin receptor type 1 in the therapeutically effective dose to a patient wherein indicated agonist of somatostatin receptor type 1 show the inhibition constant value (Ki) lower 5 nM for somatostatin receptor type 1 and its Ki value for somatostatin receptor type 1 at least by 10 times lower as compared with Ki values for each somatostatin receptors type 2, type 3, type 4 and type 5. Invention provides the maximal inhibition of unfavorable vascular proliferation at minimal symptoms of adverse effects based on the selective anti-angiogenic effect of agonist of somatostatin receptor type 1.

EFFECT: valuable medicinal properties of compositions.

12 cl, 3 tbl, 16 dwg

FIELD: veterinary science.

SUBSTANCE: invention proposes a method for elimination of radioactive elements from fattening young bulls body in regions with increased density of radioactive pollution of soil. Method involves administration in fattening young bulls the prolonged form of organic selenium-containing compound as a selenopyrane in the dose 300 mg of selenopyrane per a head by subcutaneous or intramuscular route. Method provides enhancing adaptive-protective functions, buffer capacity of antioxidant system and reducing the damage effect of radiation of animal body.

EFFECT: enhanced effectiveness of method.

3 tbl, 1 ex

FIELD: medicine, obstetrics, gynecology.

SUBSTANCE: due to instrumental technique one should pre-detect the volume of uterine cavity and its longest length against the cervix. Moreover, it is necessary to fix a woman's body in position at which the upper edge of uterine cavity bottom is below against the lower edge of uterine cervical opening till the moment of complete filling in uterine cavity with blood. With the help of a catheter one should introduce medicinal preparation heated up to +42- +45° C into uterine cavity through an opening in uterine cervix into area of cavitary bottom at the volume exceeding the half of cavitary volume. Moreover, as the above-mentioned medicinal preparation it is necessary to apply dehydrated silicone gel impregnated with equal volume of 3%-hydrogen peroxide solution. The innovation enables to interrupt postpartum hypotonic uterine hemorrhage efficiently and safely.

EFFECT: higher efficiency.

2 ex

FIELD: animal science, veterinary science.

SUBSTANCE: the suggested preparation for decreasing early embryonic lethality in cows contains vitamins A, D3 and E, potassium iodide and ACD of the 2nd fraction, sunflower or peach oil at the following ratio of components: vitamin A 15000-24000 IU, vitamin D3 20000-32000 IU, vitamin E 20-36 mg, potassium iodide 12 mg, ACD of the 2nd fraction 0.12 ml, sunflower or peach oil up to 1 ml. The innovation deals with intramuscular injection of the above-mentioned preparation during the period since the 5th to the 7th d after insemination in cows. The innovation provides decreased level of early embryonic lethality in cattle at simultaneous decrease of labor intensity of the method applied.

EFFECT: higher efficiency.

2 cl, 3 ex, 2 tbl

FIELD: medicine, homeopathy, pharmaceutical industry, pharmacy.

SUBSTANCE: invention relates, in particular, to suppository possessing immunomodulating effect and comprising coneflower, accessory substances and a base, sea-buckthorn oil, arborvitae (thuja) homeopathic essence, baptisia homeopathic essence, Timalin-1C homeopathic trituratium wherein coneflower is a component of homeopathic essence. Suppository comprises anhydrous lanolin and wax as accessory substances and cacao butter as a base wherein components are taken in the definite ration in grams per one suppository of weight 2 g. Proposed suppository possesses the enhanced immunomodulating effect and allows stimulating processes of regeneration and hemogenesis and improves processes of cellular metabolism.

EFFECT: valuable medicinal properties of suppository.

3 ex

FIELD: chemical and pharmaceutical industry.

SUBSTANCE: invention relates to natural immunocorrective preparation Tactivine in spray form. Claimed agent contains Tactivina, vitamin C and 0.14 M sodium chloride solution as solvent in specific component ratio.

EFFECT: agent of improved effectiveness and storage stability.

2 cl, 5 tbl, 4 dwg, 4 ex

FIELD: medicine.

SUBSTANCE: method involves comminuting and treating tissues with enzymes using collagenase solution in Eagle solution in Dulbecco modification, clearing from erythrocytes by means of lysing solution and filtering the obtained suspension next to it. Thymus is taken as human tissue. Collagenase of type II is applied for treating tissue with enzymes. Filtration is carried out by means of filters having pore size of 100 and 10 mcm.

EFFECT: high degree of cell mass uniformity.

FIELD: agriculture, animal science.

SUBSTANCE: one should inject thymogen intramuscularly about 60-45 d before calving thrice at 24-h-long interval at the dosage of 500 mcg/animal.

EFFECT: higher efficiency.

1 ex, 1 tbl

FIELD: medicine, immunology, veterinary science, pharmacy.

SUBSTANCE: invention concerns a medicinal agent modulating the immune response of body. Agent is made as a tablet covered by envelope. The table core comprises thymus preparation, sodium nucleate, potato starch and the accessory substances taken in the definite ratio of components. As the thymus preparation immunomodulating agent can comprise tactivine or thymaline, or thymogen. The core is covered by envelope comprising acetylphthalylcellulose, castor oil, vaseline oil, titanium dioxide, tropeolin "O" taken in the definite ratio of components. Invention expands assortment of immunomodulating agent by the development of immunomodulating agent that acts on T- and B-lymphocytes simultaneously and on macrophage link of immune system that is suitable in using by children and other patient groups for a long time wherein injection method of administration is not desirable. The immunomodulating agent "Ribotab" elicits high effectiveness providing correction of immunity by tableting agent "Ribotab" after its using in a single dose.

EFFECT: improved and valuable properties of agent.

2 cl, 2 tbl, 5 ex

FIELD: veterinary science.

SUBSTANCE: one should apply combined introduction of T-activin at the dosage of 5 mcg/kg once daily along with a single introduction of phenbendasol at the dosage of 5 mg/kg. Moreover, T-activin should be introduced for 3 d, and phenbendasol should be introduced simultaneously with the last injection of T-activin. Conditions for injecting immunomodulating agent and certain sequence of its injection along with antihelminthic preparation provide increased resistance to repeated infectioning in animals.

EFFECT: higher efficiency of combined therapy.

2 ex, 2 tbl

The invention relates to new high-performance technologies to get immunotropic sterile high quality safe drugs on the basis of sodium deoxyribonuclease, obtained from low-fat milk sturgeon, salmon, calf thymus, blood chickens

The invention relates to venereology and, in particular, to the treatment of syphilis in the absence of positive to negative reactions Wasserman
The invention relates to medicine and biotechnology, and in particular to a method for producing proteinase inhibitor from the bodies of cattle

FIELD: medicine.

SUBSTANCE: method involves applying hardware-supported treatment including computer-aided pleoptics. Programs like Eye, Crosses, Spiders and Maculotest unit are used. Laser stimulation is carried out by means of apparatus LOT-01. 30 min later, electrophoresis with 0.25% Retinal amine is carried out. Growing intensity current from 0.5 to 1 mA is used during 3-15 min.

EFFECT: enhanced effectiveness in restoring bioelectric retina activity.

4 tbl

Up!