Method for producing of biologically active preparation

FIELD: agriculture.

SUBSTANCE: method involves extracting larch and/or fir wood with organic solvent; distilling extractant for producing of aqueous emulsion of total of biologically active natural compounds. Organic solvent used is aliphatic alcohol with 1, 2, 3 carbon atoms, or acetone containing water not in the excess of 60 wt%, or mixture of these extractants. After distilling of extractant, active substance is produced, said substance being based on total of flavonoids of the following composition, wt%: dihydroquercetinic acid 70-86; dihydrokaempferol 4-12; naringenin 2-6; other compounds being the balance. Claimed preparation is preferably used in the form of aqueous emulsion comprising 2-10 wt% of active substance.

EFFECT: increased biological activity of preparation.

2 cl, 3 ex

 

The invention relates to a method of obtaining biologically active compounds from plant material, which represents the biologically active amount of natural compounds, dissolved in an aqueous solution of the emulsifier which is used to increase the productivity of agricultural plants due to application of biologically active compounds of plant origin.

There is a method of allocation of larch wood of digidrokvertsitina particularly high purity (99.5%pure)used in medicine, namely in the chemical-pharmaceutical industry (U.S. Pat. Of the Russian Federation No. 2114631, class a 61 K 35/95, C 07 D 311/32, publ. 10.07.98,). The selection is based on the extraction of timber aqueous acetone.

The disadvantage of this method is its complexity and high cost.

A method of obtaining funds for the treatment of diseases consisting in the extraction of sawdust larch wood, filtration, evaporation of the extract, the recrystallization of the target product, filtering and drying. In this way the extraction is carried out with water-acetone extract in acidic environment immediately after handling raw materials boiling water and kristallisera target product from alcohol or water-alcohol solution. The resulting solution has a composition, wt.%: dihydroquercetin 90-92, dihydrokaempferol and naringenin 8-10, and naringenin is not more than 2%. (U.S. Pat. Of the Russian Federation No. 2088256, class a 61 K 35/78, publ. 1997.08.27).

The means obtained in this way is used in medicine, and how to obtain it complicated and expensive.

The closest technical solution chosen for the prototype of the claimed method, the greater number of common features, the achieved result and the field of application is the method of obtaining biologically active drug (SILK) (U.S. Pat. Of the Russian Federation No. 2083111, class A 01 N 65/00, publ. 20.04.98).

Preparation of SILK is produced by extraction of the crushed dried green fir with a mixture of petroleum ether and ethyl acetate. The extract is treated with saturated aqueous sodium bicarbonate, acidified with hydrochloric acid and again extracted with ethyl acetate. After removal of ethyl acetate to obtain the drug, which is a sum of triterpene acids in the form of gray-green hygroscopic powder with a content of active substance 95 wt.%. This substance is soluble emulsifiers.

The product obtained according to the above method, in the form of an aqueous emulsion is used to increase the productivity of agricultural plants.

The disadvantage of this method is its complexity, a multi-stage, the use of expensive extractants.

The problem solved by the claimed method of obtaining the drug, is that more cheap and simple SPO is obom to obtain a new effective drug, not previously used in crop production.

The problem is solved in that in the method of obtaining biologically active agent for increasing the yield of agricultural plants, including extraction of plant materials with an organic solvent, obtaining water emulsion biologically active amounts of natural compounds, the wood of larch and/or fir is extracted with an aliphatic alcohol with the number of carbon atoms 1, 2, 3 or acetone containing not more than 60 wt.% water, or mixture of these extractants and then carry out the distillation of the extractant.

The drug is prepared in the form of an aqueous emulsion containing 2-10 wt.% the active substance.

After removal of the extractant get active substance (DV) of the following composition, wt.%:

- dihydroquercetin- 70-86
- dihydrokaempferol- 4-12
- naringenin- 2-6
- other connections- rest

After distillation, extractive agents may re-use them (even in the form azeotropic mixtures) in the process of extraction of the active ingredient from wood of fir and/or larch.

The main features of the proposed retrieval method is:

- the use of wood deciduous who Itza and/or fir.

- carrying out the extraction of aliphatic alcohols with the number of carbon atoms 1, 2, 3 or acetone containing not more than 60 wt.% water, or mixture of these extractants;

- production of biologically active substances (DV), the claimed composition not previously used in crop production.

The combination of these distinctive features allows you to get a new biologically active agent on the basis of flavonoids above-mentioned composition. Used aqueous solutions of the above alcohols or acetone are selective extractants for separation of the claimed active amount of natural compounds on the basis of flavonoids.

Application in plant breeding DV-based flavonoids of the claimed composition for increasing the yield and reducing the incidence of agricultural crops is new.

For justification of the criterion "industrial applicability" of the claimed method, the following specific examples of its implementation.

Example 1. 20 kg of sawdust wood fir with a moisture content of 13 wt.%, a bulk density of 0.2 kg/l was placed in the extractor stainless steel, filled with 100 l of isopropyl alcohol containing 10 wt.% water, up to a full covering of sawdust solvent. Insisted 1 h, the extract was decanted. Sawdust filled again 70 l water alcohol, insisted 1H, ekstrak is poured. Was allocated an additional 70 l of extract. The extracts were combined, heated in a distillation apparatus, kept the water isopropyl alcohol for 4 hours at the boiling temperature of the mixture until the termination of the distillation of the azeotrope. The balance in Cuba consisted of yellowish water layer and brown resinous substance. The residue is hereinafter referred to as "mixture of water/DV (DV - active substance).

From the residue was distilled water, obtained the anhydrous residue, referred to as "DV"in the amount of 1.2 kg in the form of a yellow-brown viscous mass.

Analysis of DV conducted by known methods (4)shows that it is essentially a flavonoid dihydroquercetin (3,3',4',5,7-pentahydroxyflavanone) - 80%, and dihydrokaempferol - 4%, naringenin - 3 wt.%, other connections - flavonols and glycosides of flavonoids. The quantitative content of them is not installed.

Example 2.

20 kg of sawdust larch wood with a moisture content of 18 wt.%, a bulk density of 0.25 kg/l was placed in the extractor stainless steel, filled with 100 l of ethanol containing 10 wt.% water, up to a full covering of sawdust solvent. Insisted 1 h, the extract was decanted. Sawdust filled again 70 l water alcohol, insisted 1 h, the extract was decanted. Was allocated an additional 70 l of extract. The extracts were combined, heated in a distillation apparatus, drove aqueous ethanol for 4 h at a temperature which e boiling the mixture until the termination of the distillation of the azeotrope. The balance in Cuba consisted of yellowish water layer and brown resinous substance.

From the residue was distilled water, the obtained dry residue, referred to as "DV", in the amount of 0.6 kg in the form of a light brown viscous mass.

Analysis of LW carried out as described above, showed that DV is a mixture similar in composition obtained in example 1. The content of the main components: dihydroquercetin - 86%, dihydrokaempferol - 10%, naringenin - 6 wt.%, other connections - flavonols and glycosides of flavonoids.

Example 3. Small chips of Siberian larch 15 kg with a moisture content of 9% was extracted as described in example 1, as an extractant is used a mixture of acetone and methyl alcohol in a ratio of 1:3 by weight, containing 60 wt.% water. The combined extracts were obtained at 0.42 kg viscous light brown residue with the main content, wt.%: dihydroquercetin - 86, dihydrokaempferol - 4, naringenin - 3, other substances - other.

The yield of the target product DV when using wood fir was 5.8%and 6.2%, when using larch wood - 3,2-3,6%, depending on the time and place of timber.

Preparation - standard 10%-aqueous solution of the active ingredient in a mixture of water/emulsifier.

Option 1. 1 kg of the active ingredient, obtained above, was mixed with 9 kg 10-20%-aqueous solution of the emulsifier in water. As is Aligator used soap, industrial emulsifiers OP 7, OP-10, 6 or others. The emulsion obtained is called "standard 10%emulsion DV. The drug is non-toxic (LD50 for rats of 5.2 g/kg, orally).

Option 2. From a mixture of water/LW decantation separated resinous part from the water. Distillation of the water from the sample water portion of the identified content of the active ingredient in water. Mixed resin and water part with the calculated amount of water and emulsifier and received the standard 10%emulsion of the active ingredient.

Similarly prepare a standard 2%and 5%emulsion of the active ingredient in a mixture of water/emulsifier.

From the thus prepared mixture - standard emulsion DV - prepared working solution, which was treated with vegetative plants.

1. The method of obtaining biologically active agent for increasing the yield of agricultural plants, including extraction of plant materials with an organic solvent, obtaining water emulsion biologically active amounts of natural compounds, characterized in that the wood of larch and/or fir is extracted with an aliphatic alcohol with the number of carbon atoms 1, 2, 3 or acetone containing not more than 60 wt.% water, or mixture of these extractants and then carry out the distillation of the extractant.

2. The method according to claim 1, characterized in that the drug is prepared in the form of an aqueous emulsion containing 2-10 wt.% current prophetic is TBA.



 

Same patents:

FIELD: agriculture.

SUBSTANCE: synergetic herbicide composition contains active substances such as chlorimuron-ethyl (I) and imazetapir (II) and salts thereof, used in the ratio of (I):(II)-10:1, and also contains fillers, diluents, surfactants and other functional additives needed for producing of preparation forms. Method for controlling of weed plants in sown farm crops involves using effective doses of preparations adapted for suppressing weed plants without affecting leguminous crops such as soya, feed beans, pea etc.

EFFECT: wider range of weed plant controlling means and processes.

5 cl, 2 dwg, 7 tbl, 13 ex

FIELD: herbicides, agriculture.

SUBSTANCE: herbicide composition is prepared as emulsion concentrate that is characterized by the presence of carfetrazon-ethyl, 2,4-D 2-ethylhexyl ester and organic solvent in the following content of components per 1 l of solvent: carfetrazon-ethyl, 2-50 g/l; 2,4-dichlorophenoxyacetic acid (2,4-D) 2-ethylhexyl ester, 400-700 g/l. Xylene is used as an organic solvent. For control of weeds the above said composition is taken in the dose 0.5-0.6 l/ha that is diluted and cereal crops are sprayed in bushing out phase and maize - in phase of 3-5 leaves. Invention provides decreasing consumption dose of herbicides.

EFFECT: valuable properties of herbicide.

2 cl, 2 tbl

FIELD: organic chemistry, insecticides.

SUBSTANCE: invention describes an insecticide agent comprising synergetic mixture of compound of the formula (I): wherein W, X, Z, R and G have values given in the invention claim and one of the following compounds: lufenuron, triflumuron, novaluron, fluphenoxuron, emamectin, methoxyphenozide, endosulfan, fipronil, ciromazin, indoxacarb. Insecticide activity of the proposed agent is higher as compared with sum of activities of individual active substances.

EFFECT: enhanced activity of agent.

2 cl, 23 tbl, 6 ex

FIELD: organic chemistry, herbicides.

SUBSTANCE: invention describes a herbicide agent comprising (a) herbicide active substance representing sulfonylurea of the formula (I): and/or its salts; (b) silicone surfactant chosen from the group of polyalkylene oxide-modified copolymers of dimethylpolysiloxane of the general formula: , and (c) wetting agent comprising lactic acid and its derivatives with the synergistically effective content of herbicide (a) with surfactant (b) and wetting agent (c). Also, invention describes a method for control of weeds by using the proposed herbicide agent. Proposed herbicide agent shows the excellent herbicide impact with synergetic effect.

EFFECT: improved method for weed control, enhanced and valuable properties of herbicide.

4 cl, 1 tbl, 2 ex

FIELD: organic chemistry, agriculture.

SUBSTANCE: invention describes using 3-[(substituted phenyl)carboxamido]-1,4,6-trimethyl-5-R-pyrazolo[4,5-b]pyridines of the formula (1-4): wherein in (1) R means hydrogen atom (H); in (2) R means H; X means 2-methoxyphenyl; in (3) R means chlorine atom (Cl); X means 2-methoxyphenyl; in (4) R means Cl; X means ethyl as an antidote against 2,4-dichlorophenoxyacetic acid (2,4-D) in sunflower. Invention provides expanding assortment of biologically active substances prepared by synthetic method for their using in agriculture as an antidote of herbicide 2,4-D. Method involves treatment of sunflower seedlings damaged with herbicide by proposed 3-[(substituted phenyl)carboxamido]-1,4,6-trimethyl-5-R-pyrazolo[4,5-b]pyridines.

EFFECT: valuable biological properties of antidotes.

1 tbl, 6 ex

FIELD: organic chemistry, agriculture.

SUBSTANCE: invention describes using 3-amino-(substituted amino)-1,4,6-trimethyl-5-R-pyrazolo[4,5-b]pyridines of the formula (1-2): wherein in (1) R means chlorine atom (Cl); in (2) R means hydrogen atom (H); X means 4-methylphenyl as regulators of sunflower growth. Invention provides expanding assortment of some biologically active substances prepared by synthetic method for their using in agriculture as regulators of plants growth.

EFFECT: valuable biological properties of substances.

1 tbl, 4 ex

FIELD: organic chemistry, antidotes, agriculture.

SUBSTANCE: invention describes using N-substituted 3-cyano-4,6-dimethyl-5-chloropyridyl-2-sulfonylamides of the formula (1-2): wherein in (1) R means benzimidozolyl-1; in (2) R means furfurylamino-group as antidotes of herbicide 2,4-dichlorophenoxyacetic acid in sunflower. Invention provides expanding assortment of biologically active substances prepared by synthetic method for their using in agriculture as antidotes against 2,4-D. Method involves treatment of sunflower seedlings damaged with herbicide by proposed N-substituted 3-cyano-4,6-dimethyl-5-chloropyridyl-2-sulfonylamides.

EFFECT: valuable biological properties of antidotes.

1 tbl, 3 ex

FIELD: agriculture, in particular, suppression of weeds with developed triazine resistance resulted from selection stress acting upon weed population and caused by repeated application of triazines.

SUBSTANCE: method involves introducing into said weed locus combination of mesotrione and triazine, selected from the group consisting of atrazine, cyanazine, propazine, terbutilazine, trietazine, simazine and metribuzine. Mesotrione is introduced in an amount of from 20 to 210 g/hectare, triazine in an amount of from 0.1 to 2 kg/hectare.

EFFECT: increased efficiency of said combination as compared to that of mesotrione in case said combination is used with regard to triazine tolerable weeds.

3 cl, 9 tbl, 6 ex

FIELD: organic chemistry, herbicides.

SUBSTANCE: invention relates to a compound of the general formula [I]: wherein R1 and R2 can be similar or different and each represents (C1-C10)-alkyl group; each among R3 and R4 represents hydrogen atom; R5 and R6 can be similar or different and each represents hydrogen atom or (C1-C10)-alkyl group; Y represents 5-6-membered aromatic heterocyclic group or condensed aromatic heterocyclic group comprising one or some heteroatoms chosen from nitrogen atom, oxygen atom and sulfur atom wherein heterocyclic group can be substituted with 0-6 of similar or different groups chosen from the following group of substitutes α, and so on; n means whole values from 0 to 2; [Group of substitutes α]: hydroxyl group, halogen atoms, (C1-C10)-alkyl groups, (C1-C10)-alkyl groups wherein each group is monosubstituted with group chosen from the following group of substitutes β, (C1-C4)-halogenalkyl groups, (C3-C8)-cycloalkyl groups, (C1-C10)-alkoxy-groups, (C1-C10)-alkoxy-groups wherein each group is monosubstituted with group chosen from the following group of substitutes and so on; [Group of substitutes β]: hydroxyl group, (C3-C8)-cycloalkyl groups that can be substituted with halogen atom or alkyl group, (C1-C10)-alkoxy-group, (C1-C10)-alkylthio-groups, (C1-C10)-alkylsulfonyl groups, (C1-C10)-alkoxycarbonyl groups, amino-group, carbamoyl group (wherein its nitrogen atom can be substituted with similar or different (C1-C10)-alkyl groups), (C1-C6)-acyl groups, (C1-C10)-alkoxyimino-groups, cyano-group, optionally substituted phenyl group; [Group of substitutes γ]: optionally substituted phenyl group, optionally substituted aromatic heterocyclic groups, cyano-group. Also, invention relates to herbicide comprising derivative of isoxazoline of the formula [I] as an active component or its pharmaceutically acceptable salt. Invention provides the development of isoxazoline derivative possessing the herbicide activity with respect to resistant weeds, selectivity for cultural crop and weed.

EFFECT: valuable herbicide properties of substances.

18 cl, 24 tbl, 106 ex

FIELD: agriculture, in particular method for increasing of beet yield and sugar content.

SUBSTANCE: seeds are treated by spraying in leaf clamping step with titanium tetrachloride diluted in dimethylsulfoxide (DMSO). Concentration of DMSO is 0.1-2 %; titanium tetrachloride consumption is up to 50 g per 1 hectare of seeds. Ratio of titanium tetrachloride:dimethylsulfoxide is 100-200 l per 1 hectare. Treatment is carried out twice in vegetation period, namely the first treatment is carried out in leaf clamping step and the second one in 15-20 days before harvesting of root crops.

EFFECT: increased beet yield and sugar content.

1 tbl, 1 ex

FIELD: agriculture.

SUBSTANCE: lignosulfonate-based composition includes glycerol in amount of 6.2-32.0 % and optionally contains microelements in amount of 0.5-4.0 % based on lignosulfonate mass.

EFFECT: composition with improved flexibility.

2 cl, 2 tbl, 2 ex

FIELD: agriculture.

SUBSTANCE: invention relates to preparations obtained by pharmacopoeia method from overground and underground parts of calamus (Acorus calamus L.) for controlling of freshwater mollusk.

EFFECT: enhanced assortment of effective agents for controlling of freshwater mollusk.

6 ex

FIELD: protective agent for houseplants.

SUBSTANCE: claimed agent contains (mass %): dried calendula flowers as active ingredient 1.67-1.86; milk as solvent 95.35-96.25; sand sugar as adhesive agent 2.08-2.79.

EFFECT: protective agent of increased effectiveness.

1 tbl, 3 ex

FIELD: forestry and agriculture.

SUBSTANCE: claimed stimulator contains running waters from distillation of essence oils such as fir, spruce, pine, larch, ledum, and birch.

EFFECT: agent of increased effectiveness; enhanced assortment of plant growth stimulators.

2 cl, 2 tbl

FIELD: agriculture.

SUBSTANCE: method involves fermentation process of the plant fenestrate Saint-John's-wort vegetable extract at temperature 35-50°C with addition of whey in the mass ratio = (1:1)-(1:2) followed by thermal denaturation of inert proteins, their separation by centrifugation and sterilization of the preparation by autoclaving. Invention provides enhancing activity and stability of the preparation.

EFFECT: improved preparing method of stimulator.

2 tbl, 7 ex

FIELD: agriculture.

SUBSTANCE: claimed fungicide composition contains iprodione and vegetable-origin oil having increased drying ability and iodine number of above 90. Weight ratio of oil/iprodione is from 0.15 to 1.6. Treatment method includes application of effective and non-phytotoxic amount of composition onto plant overground parts.

EFFECT: composition of decreased phytotoxicity.

8 cl

FIELD: agriculture.

SUBSTANCE: claimed agent contains (per 1 l): a) 10-33 % of anionic surfactants, b) 5-18 % of non-ionic surfactants, c) 2-20 % of ethanol or methylated alcohols, or apple vinegar, d) 25-60 % demineralized aqueous solution, e) catalytic enzymatic glycosides from vanilla, almond, or strawberry extracts, f) 0.01-1 g of food-grade dye, g) 0.01-5 g of lanoline, or 0.5-15 ml of glycerol, or 0.5-5 ml of paraffin oil.

EFFECT: agent for plant hardening of improved effectiveness.

20 cl, 4 ex

FIELD: agriculture.

SUBSTANCE: invention relates to composition used in treatment of gladiolus corms for prophylaxis of diseases and comprising the following components, wt.-%: potato germs sap, 0.00002-0.00003; garlic sap, 0.00002-0.00003; chrysanthemum sap, 0.000025-0.000035; silver nitrate, 0.000025-0.00003; succinic acid, 0.000045-0.000055; potassium permanganate, 0.018-0.025; tansy infusion, 0.00018-0.00025, and water, the balance. Invention provides enhancing productivity and quality of production.

EFFECT: valuable properties of composition.

2 tbl

FIELD: agriculture.

SUBSTANCE: method involves treatment of cuttings with extract prepared of perfoliate rosin-weed leaves (Silphium perfoliatum L.) as an aqueous solution in the concentration 2-5 g/l. Invention provides preparing nursery plants showing the more developed above ground part. Invention is designated for using in agriculture field.

EFFECT: improved stimulating method.

2 tbl, 2 ex

FIELD: agriculture.

SUBSTANCE: invention proposes using the plant roof avens (Geum urbanum L.) preparations that are prepared by pharmacopoeia method. Rood avens preparations in form of powder or extract are used for treatment of fresh-water mollusks inhabitation sites on the pasture or on the lowered pond bed that provides practically total eradication of mollusks and their egg mass for 3-6 days. Invention allows expanding assortment of agents and to realize the indicated designation.

EFFECT: valuable properties of agent.

6 ex

FIELD: organic chemistry, chemical technology, agriculture.

SUBSTANCE: invention relates to a method for preparing the preparation comprising triterpenic acid water-soluble salts and additionally added protein-containing product and vegetable raw, the source of triterpenic acids taken in the following ratio of components, wt.-%: protein-containing product, 10-17; triterpenic acid sodium salts, 4-5, and vegetable raw, the balance. Method involves mixing triterpenic acid-containing vegetable raw with the protein-containing product taken in the ratio = (9-11):(1-2), mechanical-chemical treatment of this mixture in activator device, mixing of prepared semi-finished product with sodium carbonate or sodium hydrocarbonate taken in the ratio = (92-97):(3.5-8.3) and repeated treatment in the activator device. Method involves applying flow-type ball vibration-centrifugal or ellipse-centrifugal mills as the activator device that provide the acceleration of milling bodies up to 170-250 m/c2 and time for treatment for 1.5-3 min. Invention provides simplifying the process and the complex processing waste in lumber industry.

EFFECT: improved preparing method.

6 cl, 1 tbl, 6 ex

Up!