Method for treating bilateral purulent tumor-like neoplasms of uterine adnexa
FIELD: medicine, gynecology.
SUBSTANCE: before operation it is necessary to sample patient's blood to divide it into plasma and blood cells. Blood plasma should be divided into three parts and frozen. After operation one should intravenously reinfuse by drops erythrocytic mass incubated for 30 min at 37° C with 2 g lendacyn followed by further lendacyn injection per 1g intramuscularly during the next 6 d. Blood plasma should be portionally defrosted, incubated at 37° C with: 10000 U trasilol, 0.15 g lisozyme, 0.02 g novocain, after incubation it is necessary to add 100 ml gelatinol to be reinfused for a patient on the 2nd, 4th and 6th d against the onset of therapy course through microirrigator withdrawn out of posterior arch during operation. The innovation provides combined impact of antibiotics upon pathological focus biotransformed with erythrocytic mass and local immunomodulating and resolving effect and, thus, decreased formation of adhesions, fistulas and cicatrices.
EFFECT: higher efficiency of therapy.
The invention relates to medicine, namely to gynecology, and can be used for the treatment of diseases of the pelvic organs in women.
It is known that the method of choice for inflammatory tumors of the uterus that are not related to abortion and the presence of intrauterine device, is a supracervical amputation of the uterus with appendages (Beznoshchenko G.B. non-operative gynecology. M: Honey. book. Nizhny Novgorod: publishing house of the ngma. 2001. - S). It is necessary to completely remove all tissue inflammation of education. A required component surgical benefits in these patients is draining. To limit churn content combined plugging and drainage. Using flow-drainage system (Winternitz tube), which is tightly connected with a cavity or multiple tubes creates adequate outflow. Tampons and drains better output through separate incisions rather than through surgical wound is clearly labeled and marked in the Protocol operations (skin fix seams). The drainage is carried out through bilateral contraditory. In the postoperative period is antibiotic therapy. Thus prescribe broad-spectrum antibiotics in combination with metronidazole.
However, the drainage tube through the anterior abdominal wall into the bottom corner of paleoposition the second wound is associated with a number of inconveniences and complications first of all obstructed outflow of fluids as the sloping part of the belly are significantly below the level of the anterior abdominal wall. The drain pipe is a foreign body, and this may result in the formation of fistulas. Often after suffering a suppurative inflammation of the uterus and surgery complications (scleroses, scars, adhesions). Therefore, patients need additional non-drug treatment (heat absorbing therapy) for at least a year.
There is a method of treatment of bilateral purulent inflammation of the uterine appendages, including surgical treatment in the amount of eksterpatsii or supracervical amputation of the uterus with appendages and post-operative rehabilitation, chosen as a prototype (Malevich SURDS, Rusakevich PS Treatment and rehabilitation of gynecological diseases. - Mn: Wyscc., 1994. - S). Surgical intervention is mandatory drainage. The predominant method consider the introduction of drainage transvaginal through the open dome of the vagina after the removing of the uterus. Drains, microirrigation enter for carrying out intra-abdominal drip infusion with continuous evacuation of the pathological exudate on the type of peritoneal dialysis using the Winternitz silicone tube and vacuum device OP-01. antibacterially therapy in the postoperative period is recommended to be conducted simultaneously in two or more antibiotics. Recommended following use of antibiotics: intramuscular, intravenous, through drainages, in cases of severe endolymphatic.
However, when choosing a method of drug therapy of various diseases of the greatest importance localization and extent of the process, as well as aspects of applied pharmacokinetics of drugs, including determining the speed of binding with plasma proteins (in all ways), time to reach the maximum concentration in blood plasma half-life, the time of complete removal. The definition of these parameters and their comparison with the dynamics of therapeutic effect allow you to set the optimal doses and modes (frequency and duration) of the drug, to assess the advantages of different ways of its introduction. Getting into the blood, the medicine is not only in the lesion, but also spreads throughout the body by binding part with healthy tissues. The use of multiple antibiotics leads to the development of dysbacteriosis and immunosuppression, which requires additional treatment measures. The opinion that the introduction to the lymphatic system drugs weakens their General toxic effect, can create a false impression of harmlessness endolymphatic infusion of high doses of toxic drugs. And the diffusion in the lymphatic vessel maximum dose, for example, gentamicin creates in regional and some other nodes in the concentration above therapeutic approximately 25 times. Naturally, this leads to a pronounced local toxic effect, suppresses not only pathogenic start, but the protective functions of the lymphatic system. Characteristically, and thus remain lymph nodes even on the paths of motion of the antibiotic through the lymphatic system, in which the accumulation of the drug may be missing. In other nodes, in which he acts, its toxic effect is compounded by the extremely slow nature of the lymph flow compared to the flow that determines the prolonged contact of the drug with the surrounding tissue. The procedure of endolymphatic administration of drugs involves the stage of "minor" surgery, consisting in the detection and exposure of the lymph vessel of the foot. However, varicose blood vessels of the lower extremities, which often occurs in older women, this procedure is not feasible. Contraindicated endolymphatic administration of drugs and in violation of the blood coagulation system. Frequent complication remain lymphangitis.
The aim of the present invention is to improve the treatment results of patients with purulent-like formations at Atkov of the uterus.
This goal was achieved by the fact that patients with purulent-inflammatory formations of the uterus the first stage performs the operation in the amount of supracervical amputation of the uterus with appendages and the formation of the drainage system for the removal of the drainage tube through the cervical canal and microirrigation through the rear arch. The second stage of treatment in patients with established drainage system are drug therapy. To do this, from the cubital vein of the patient on an empty stomach take 250 ml of blood into a sterile standard vial logician, by the method of sedimentation get the blood plasma. RBC mass after incubation with antibiotics return the patient intravenously. In the next day antibiotics injected intramuscularly daily for up to 7 days. The blood plasma is divided into 3 portions of 50 ml and frozen at -18°C. In 2, 4 and 6, the day the blood plasma portion thawed, incubated with a mixture of drugs and is re-infused to the patients through microirrigation led out through the rear arch.
Analysis of the known methods of treatment of patients with purulent-inflammatory formations of the uterus and developed enables us to conclude that the proposed method is new, which is a combination of systemic intravenous antibiotics, biotransforming erythrocyte suspension,and entered directly into the bloodstream, with therapy applied directly to the area of distribution of the purulent process, which allows to influence all not visible to the eye lesions remaining in the abdominal cavity, and which has an immunomodulatory and absorbing effects.
In the available sources of information Russia, CIS and abroad guidance on this method of improving the immediate results of treatment of patients with purulent-inflammatory formations of the uterus with the simultaneous objective of reducing the number of complications associated with the formation of adhesions, fistula and additional scarring, no.
The invention is industrially applicable, as may be repeatedly reproduced and multiplied in hospitals surgical profile.
The method is as follows.
After conducting a bimanual, ultrasonic research of organs of a small pelvis and cytological analysis, detection of common chronic inflammatory process of the uterus of the patient first stage of surgical treatment in the amount of supracervical amputation of the uterus with appendages. Before the operation the patient from the cubital vein on an empty stomach take 250 ml of blood in a standard sterile vial logician. Immediately after supracervical amputation of the uterus with appendages from the ventral floor of the STI in the channel of cervical stump down the vagina hold the drain tube with a diameter of 0.5-0.7 cm and length 50 cm The tube side of the vagina capture clip and stretch until you exit it. The upper end of the tube from the abdominal cavity in end position behind the upper edge of the cervical stump so that after suturing the tube is located above the line of stitches on 1,5-2 see Drainage through the cervical canal combined with microirrigation derived through the rear arch. For better fixation of the drainage tubes use horizontal jumper - a ligature, which is based on the posterior wall of the cervical stump, keeps drains in a given direction. Microirrigation is to take stock solutions of drugs into the abdominal cavity and the drainage led out through the cervical canal, for adequate drainage of fluid from the abdominal cavity.
By the method of sedimentation taken before surgery, the blood is separated into plasma and cell suspension. RBC mass incubated for 30 min at 37 ° °With 2 g of indocina and return the patient intravenously immediately after the operation, in the next 6 days Lindzen continue to enter 1 g intramuscularly. The blood plasma is divided into three portions of 50 ml and frozen at -18° (freezer household refrigerator). At 2, 4 and 6 days from the beginning of the treatment portion of the blood plasma is thawed, incubated at 37 ° °30 min with a mixture of drugs is a war preparations: 10,000 units the trasilol, 0.15 g of lysozyme, 0.02 g of novocaine. After incubation in a mixture of plasma and medicines add 100 ml of Gelatines and the patient is re-infused after microirrigation led out from the rear of the set.
In the available sources of information Russia, CIS and abroad guidance on this method of treatment of patients with purulent-inflammatory granulomas diseases of the uterine appendages we are not found.
For proof, here is the excerpt from the history of the disease, confirming the clinical effect of the treatment approach.
An example of clinical application
Patient I., born in 1946, istby. No. 5916/e received 17.06.97 with an open reception in RNII complaining of intense abdominal pain with irradiation to the rectum, blood discharge from the genital tract, chills, body temperature of 37.8°With frequent urination.
Considers herself a patient in the course of 2 months, when after medical abortion appeared and intensified these complaints, in spite of the place of residence conservative detoxification and antibiotic therapy.
At admission the patient's condition moderate, pale skin. The vesicular breathing, tachycardia - the heart rate of 110 beats per 1 minute, rhythmic HELL 100/60 mm RT. Art. Tongue moist, coated with white bloom. The stomach is involved in the act of breathing, soft. Palpation Bo is znanosti and small muscles of the anterior abdominal wall into the ileum departments, where is defined weakly positive symptom Shchetkina-Blomberg.
Genital status: cervix formed, without tumors of the cervical canal serous, sero-purulent discharge. Is determined by the tension of the arches. The uterus is a bit more than normal, its contours are not clearly defined. Pain during examination and traction for the cervix. The appendages of the uterus increased on both sides, bumpy, painful when researching more on the right.
Ultrasound of the pelvic organs from 17.06.97 - the size of the uterus 9,8×6,3×4,4 see the uterus expanded liquid content. In the zone of the median M-echo defined Hyper - and hypoechoic areas of various sizes and shapes. In the basal layer of the endometrium from 0.1 - 0.2 cm hyperechoic areas of fibrosis and calcification. Appendages increased right 8,1×6,4×5.1 cm to the left of 6.7×5,4×5,3 see
HB 124 g/l, er - 3,8×1012/l CPU 1,0; lake. - 9,4×109/l, EOS. - 1%, Miele. - 2%; PAL. - 21%, segm. - 55%, limp -16%, mon - 5%, ESR 24 mm/hour.
Diagnosis: bilateral salpingo-oophoritis, metroendometrit. Given the age of the patient and the prevalence of the process, shown surgical treatment.
Before the operation the patient from the cubital vein on an empty stomach took 250 ml of blood in a standard sterile vial logician. Surgical treatment carried out in volume supracervical amputation of the uterus with appendages. Directly to the public after supracervical amputation of the uterus with appendages from the abdominal cavity in the canal of cervical stump down the vagina hold the drain tube with a diameter of 0.5-0.7 cm and length 50 cm The tube side of the vagina capture clip and stretch until you exit it. The upper end of the tube from the abdominal cavity in end position behind the upper edge of the cervical stump so that after suturing the tube was placed above the line of stitches on 1,5-2 see Drainage through the cervical canal combined with microirrigation derived through the rear arch. For better fixation of the drainage tubes were used horizontal jumper - ligature, which is based on the posterior wall of the cervical stump, kept the drains in a given direction. Microirrigation served to take stock solutions of drugs into the abdominal cavity and the drainage led out through the cervical canal, for adequate drainage of fluid from the abdominal cavity. Histological analysis: No. 479929-34 - chronic oophoritis with exacerbation; No. 479938-9 chronic inflammation with aggravation.
By the method of sedimentation taken before surgery, the blood was separated into plasma and cell suspension. RBC mass after 30 min incubation at 37 ° °With 2 g of indocina intravenously introduced the patient immediately after the operation. In the next 6 days (18.06. at 23.06. inclusive) Lindzen was administered 1 g intramuscularly. The blood plasma was divided into three portions of 50 ml and frozen at -18° (freezer household x is Hladilnika). In the second (18.06.97), fourth (20.06.97) and sixth (22.06.97) day from the start of treatment, each portion of the blood plasma were thawed, incubated at 37 ° °30 min with a mixture of drugs: 10,000 units of trasilol, 0.15 g of lysozyme, 0.02 g of novocaine. After incubation in a mixture of plasma and drugs were added 100 ml of Gelatines and repusively patient through microirrigation led out from the rear of the set.
The clinical recovery of the patient. Exacerbation of chronic inflammatory process is not marked for 7.5 years.
The proposed method was carried out treatment of 34 patients with purulent-inflammatory granulomas diseases of the genitals. Before treatment and after its completion, all patients were examined in the mirrors, rectovaginal examination, General and biochemical blood tests, chest x-ray study of the kidneys, ultrasound examination of the pelvic organs, biopsy Cytology.
The technical result from the use of the Method of treatment of bilateral suppurative granulomas formations of the uterus" is that:
- patients tolerate the procedure without complications;
- decrease the toxic side effects of drug therapy;
- achieved regression of the pathological focus and bleicherode time;
- no fistulous passages, additional scarring, adhesions.
Treatment of bilateral suppurative granulomas formations of the uterus, including the operation and administration of drugs, characterized in that before the operation the patient to take blood, divide it into plasma and the formed elements, plasma is divided into three parts and freeze, operative treatment is complete removal of drainage through the cervical canal and the rear arch, after the operation is re-infused intravenously RBC mass, incubated 30 min at 37°With 2 g of mendacino, then continuing the introduction of indocina 1 g intramuscularly in the next 6 days, the blood plasma portion thawed, incubated at 37°With: 10,000 units of trasilol, 0.15 g of lysozyme, 0.02 g of novocaine, after incubation, add 100 ml of Gelatines and the patient is re-infused after microirrigation led out from the back arch, the procedure is carried out in the second, fourth and sixth days of starting treatment.
FIELD: organic chemistry, biochemistry, medicine, pharmacy.
SUBSTANCE: invention relates to novel amino- and hydroxy-derivatives of phenyl-3-aminomethylquinolone-2 of the general formula (1):
wherein R1, R2, R3 and R4 are independently similar or different and R1 is chosen from hydrogen atom (H), Alk, OAlk; R2 is chosen from H, Alk, OAlk, -OCF3; R3 is chosen from H, Alk, OAlk, -SCH3; R4 is chosen from H. Alk, OAlk, or R2 and R3 are chosen from -(CH2)3, -OCH2O-, -OCH2CH2O-; R5 means H or Alk; R6, R7 and R9 mean H; R8 is chosen independently from the following substitutes:
wherein n = 1, 2, 3; Het represents furan; R represents hydrogen atom or alkyl. In case of hydroxy-derivatives at least one among R6, R7, R8 or R9 is -OH and other represent H. Also, invention relates to methods for synthesis of these compounds and to a pharmaceutical composition based on these compounds inhibiting activity of NO-synthase. Invention provides preparing novel compounds and pharmaceutical compositions based on thereof in aims for treatment of diseases associated with hyperactivity of phagocytizing cells, for example, rheumatic arthritis, asthma and others.
EFFECT: improved preparing method, valuable medicinal and biochemical properties of compounds and pharmaceutical composition.
32 cl, 1 tbl, 132 ex
SUBSTANCE: method involves determining lymphocyte CD 56+ in peripheral blood before planned pregnancy. The content exceeding norm, lymphocytoimmunotherapy courses are applied with donor lymphocytes. The courses are applied at the fifth-eighth day of fertility cycle until normal lymphocyte CD 56+ values become restored. Pregnancy being proved, lymphocytoimmunotherapy courses are applied with ultrasonic examination results having in view. Choriohypoplasia being available, the courses are applied every 2 weeks. Chorion thickness being in conformity with gestation age, the courses are applied every 3-4 weeks to 12-14 pregnancy weeks.
EFFECT: adequate chorion and placenta development.
FIELD: pharmaceutical industry.
SUBSTANCE: claimed composition for sublingual application contains apomorphine as active ingredient and succinic acid as apomorfine stabilizer and filler, as well as other additives.
EFFECT: sublingual tablet with sufficient strength and resolvability.
4 cl, 1 tbl, 2 ex
FIELD: medicine, gynecology.
SUBSTANCE: invention relates to a method for correction of vegetative symptoms in climacteric syndrome in women with ischemic disorders in cerebral circulation. Method involves administration of mexidol in the dose 125 mg, 3 times per a day for 8 weeks, and alzolam in the dose 0.25 mg, once per a day, 30 min before sleeping for 4 weeks. Method represents the alternative substitution hormonal therapy and provides the simultaneous correction of vegetative disorders in climacteric period and ischemic damages of the cerebral circulation.
EFFECT: improved method of syndrome correction.
FIELD: medicine; medical engineering.
SUBSTANCE: method involves exposing cavernous bodies of compulsory erected penis and urogenital system tissues to physical factor complex action as pulsating pressure-mediated local negative pressure therapy and low intensity red bandwidth laser radiation. Noninvasive penis treatment with contrasting temperatures and vaso-active exogenous substances like ozone/NO-air gas mixture are additionally applied. The device has transparent penis flask having throttling orifice, cover-holder, light-emitting diode matrix, vacuum pump for creating negative pressure pulses in the penis flask, laser radiation sources, magnetic and electric field sources and rectal heater. The device has source of exogenous vaso-active substance like ozone/NO-air gas mixture and source for heating the atmospheric air. On-off valve having control solenoid coupled to deactivator of waste exogenous vaso-active substance like ozone/NO-air gas mixture is mounted in the throttling orifice of the penis flask. The penis flask is connected to movable upright via carrier holder and hinged articulation system having movable upright allowing varying penis flask orientation with height and spread angle relative to the penis.
EFFECT: enhanced effectiveness of anti-inflammatory treatment; improved erection stimulation mechanisms.
2 cl, 8 dwg, 1 tbl
FIELD: medicine, obstetrics and perinatology.
SUBSTANCE: invention relates to a method for prophylaxis of the pregnancy gestosis. Method involves administration of aspiring beginning from the second trimester of pregnancy and additional administration of the preparation "Omeganol" in the dose 2 capsules, 3 times per a day for 1 month. Then 2 months later the course of administration of the preparation "Omeganol" is repeated. Method provides improving circulation in the system mother-placenta-fetus and anticoagulant and fibrinolytic effects that results to decreasing hypercoagulation that accompanies gestosis in pregnancy.
EFFECT: improved method of prophylaxis of gestosis.
3 tbl, 2 ex
FIELD: veterinary science.
SUBSTANCE: invention relates to prophylaxis of sterility in cows. Method involves a single intramuscular administration of sodium selenite as 0.5% solution in the dose 10 ml 20-25 days before calving, and tetravit in the dose 10 ml twice before calving and twice after calving with break for 10 days. Succinic acid in mixture with ascorbic acid in the ratio = 1:1 is administrated orally every day for 20-25 days before and after calving in the dose 0.5-1.5 g per a head. Method provides activation of system of antioxidant protection and natural resistance that results to prophylaxis of sterility in cows that is based on selection of doses and regimen of administration of agents used.
EFFECT: improved method of sterility prophylaxis.
6 tbl, 3 ex
FIELD: medicine, gynecology.
SUBSTANCE: patients should be suggested to take radon procedures as baths, gynecolopgical irrigations, microenemas, laserotherapy in autoresonance mode at altering frequency of laser apparatus independently against frequency characteristics of human body, at wave length of 0.89 mcm, power of 10 W onto area of uterine projection in case of genital endometriosis and uterine myoma at exposure of 15 min and onto mammary glands in case of diffuse proliferative mastopathies. Impact fields should be chosen by sectors ranged 10-16 per 10-15 sec for each impact field. Local therapy should be conducted about 2.5-3 h after taking radon procedures successively, not earlier. The first stage - baths with malavit at 1:5 dilution at exposure of 15 min. The second stage - after removing the solution out of vagina it is necessary to introduce malavit gel at a tampon for 4-6 h. The whole therapy should be carried out together with internal intake of mineral water 30-60 min before meals thrice daily per 3.5 mg/kg body weight. The innovation enables to achieve prolonged remission, normalize hormonal level in blood and duration of the second phase of the cycle and decrease cholesterol level in blood.
EFFECT: higher efficiency of correction.
1 dwg, 4 ex
FIELD: medicine, gynecology.
SUBSTANCE: invention relates to a method for treatment of fallopian-peritoneal form of infertility suffering with chronic endometritis. After carrying out reconstructive endoscopic operations in small pelvis cavity under control of histerscope tool a medicinal cocktail is administrated by intrauterine route by the following schedule: at the first and third days of treatment - prednisolon in the dose 30 mg, dioxydin in the dose 50 ml of 0.5% solution, metrogil in the dose 5.0 ml, lydaze in the dose 32 IU, antibiotic - "Makmimor" suppository before night by vaginal route and vagina syringe with matricary solution in the morning. At the second and fourth days of treatment prednisolon is administrated in the dose 30 mg by intrauterine route, "Instillagel" in the dose 6-11 mg into vagina posterior fornix in the dose one tampon with 20 ml of Vishnevsky's ointment. On the fifth, seventh and ninth days dioxydine is administrated in the dose 50 ml of 0.5% solution by intrauterine route and also 5.0 ml of metrogil, 0.5 ml of cycloferon, 32 IU of lydaze, antibiotic, and "Makmimor" suppositories are administrated by vaginal rout before night, and vagina syringe with matricary solution is carried out in the morning. On sixth, eighth and tenth days of treatment "Instillagel" is administrated in the dose 6 ml by intrauterine route, dimexide in the dose 5 ml and tampon with 20 ml of Vishnevsky's ointment is administrated into vagina posterior fornix before night. Since the 1-st day of treatment wobenzym is prescribed in the dose 5 tablets, 3 times per a day for 14 days and cyclic hormonal therapy for 3 months. Method provides the full-value elimination of pathogens from uterine cavity, normalization of vagina biocenosis and recovery of the menstrual function.
EFFECT: improved and enhanced method of treatment.
4 tbl, 1 ex
FIELD: medicine, urology.
SUBSTANCE: method involves carrying out the echo-dopplerographic study of the male genital system before physiotherapy course followed by carrying out pharmaco-echo-dopplerography of genital system vessels by using vardenofil in an average single dose 10-20 mg given in 30-45 min after oral administration of the preparation. In case of increasing linear blood stream rates in the genital system vardenofil is prescribed in one-half single dose, once time per 24 h additionally to physiotherapy during all course of treatment, and also prostatilen, rectal suppositories and microenemas and every day massage of prostate by fingers. Also, the rational psychotherapy of "syndrome in waiting failure" and "examination syndrome" is carried out; then administration of vardenofil for 6 months is prescribed in one-half single dose in each other 24 h or 2 times per a week and then similar course is repeated 4-5 times with break for 6 months. Method provides improving results of treatment due to carrying out the selective vasoactive therapy of genital system in patients suffering with chronic prostatitis and with parallel decreasing erectile and psychosexual function. Invention can be used in treatment of patients with chronic prostatitis in combination with copulative disorders.
EFFECT: improved and enhanced treatment method.
6 tbl, 1 ex
FIELD: biotechnology, biochemistry, enzymes.
SUBSTANCE: invention reports about preparing a polypeptide possessing with the biological activity of enzyme protein-histidine-phosphatase and a polynucleotide encoding this enzyme also. Based on the polypeptide a pharmaceutical preparation is prepared using in pathological states associated with disturbance function of protein-histidine-phosphatase. Also, antibodies showing specificity to sites of indicated polypeptide are prepared. The use of the invention provides study of the N-phosphorylation process, carrying out the diagnosis of pathology of cellular regulation and growth cells and to regulate pathological states associated with disturbance in function of protein-histidine-phosphatase. Invention can be used in medicine and pharmacy.
EFFECT: valuable medicinal properties of enzyme, improved preparing method.
11 cl, 3 tbl, 10 dwg
FIELD: medicine, veterinary science.
SUBSTANCE: the present innovation deals with treating malignant tumors. For this purpose, its is necessary to provide a blood supply of an agent that destroys extra-cellular blood DNA. This agent should be introduced in dosages providing alteration of electrophoretic profile of extra-cellular blood DNA. Agent, also, should be introduced at the dosages and modes that provide the level of DNA-hydrolytic activity of blood plasma measured in blood plasma being above 150 Kunz units/l plasma during totally above 12 h daily. Therapy may last without intervals for 2 d, not less. As an agent destroying extra-cellular blood DNA one may apply DNAse, in peculiar case, bovine pancreatic DNAse or recombinant human DNAse. The innovation suggests, also, to apply and agent that binds extra-cellular blood DNA, for example, anti-DNA antibodies. The method provides low-toxic and efficient treatment of tumors, particularly at prolonged, even one's life-long therapy with preparations mentioned.
EFFECT: higher efficiency of therapy.
10 cl, 7 ex, 6 tbl
SUBSTANCE: method involves administering pancreatine and/or a digestive enzyme mixture acting like pancreatine, in addition to insulin therapy. The remedy is introduced at a dose of 40000 lipase units when eating and 20000 lipase units between food intakes. Enzyme treatment is carried out on the background of patient fecal elastase-1 level not exceeding 100 mcg/g of patient stool.
EFFECT: enhanced effectiveness of glycemia control.
4 cl, 5 tbl
FIELD: medicine, cosmetology.
SUBSTANCE: one should introduce enzymes-containing liposomes of DNAase activity into skin and/or subcutaneous fiber, moreover, one should apply multi-layer MLV-liposomes at their size ranged 500-2000 nm at temperature of phase transition of liposomal membrane being 30-60 C, moreover, one should deliver and deposit an enzyme into intercellular space, and as enzymes with DNAase activity one should apply endo- and/or exonucleases that destroy single and/or double stranded DNA. The innovation prevents the damage of intracellular DNA of alive skin cells and/or its adnexa and/or subcutaneous fiber.
EFFECT: higher efficiency of prophylaxis and correction.
4 ex, 4 tbl
FIELD: medicine, biotechnology, pharmaceutical industry, veterinary science.
SUBSTANCE: method involves addition of tris-HCl buffer (pH 7.5) containing 0.1 M of lysine and 5 mM of EDTA to the Cohn's fraction followed by incubation and centrifugation. Prepared suspension is incubated with 10% PEG-3000 and after centrifugation the solution is applied onto column with lysine-Sepharose at pH 7.5 and plasminogen is eluted with buffer solution at pH 3.5 containing 0.1 M of glycine, 30 mM of lysine and 5 mM of caprylic acid. Then plasminogen is incubated with streptokinase in tris-buffer (pH 7.5) containing 15 mM of lysine, 10 mM of ε-aminocaproic acid and 50% of glycerol. Then method involves chromatography on column with benzamidine-Sepharose at pH 8.5 and elution with buffer at pH 3.5 containing 0.1 M of glycine, 30 mM of lysine, and the end product is lyophilized that comprises about 30 IU/ml of plasmin with purity above 98%, at least 10 mM of lysine and 10 mM of glycine in an aqueous solution at pH about 3.5. The prepared product shows apyrogenic property, absence of toxicity in experiments in laboratory animals and it doesn't cause allergic or other adverse response reactions in intracutaneous or intravenous administrations, and the preparation doesn't cause defects in the vision field after its an intraorbital route of administration.
EFFECT: improved preparing method, valuable medicinal properties of plasmin.
7 cl, 1 ex
FIELD: medicine, neurology.
SUBSTANCE: method involves intravenous administration of autolymphocytes treated with an immunomodulating agent by extracorporal method using cycloferon (250 mg) as an immunomodulating agent. Simultaneously, the following medicinal mixture comprising lidocaine, 100 mg; lidazum, 32 U; dexamethasone, 4 mg; leukinferon, 10 000 U; 40% glucose solution, 4 ml is administrated into interspinal ligaments of spinal column at levels corresponding to thoracal and lumbar enlargements of the spinal cord. The procedure is repeated three times with interval for 48-72 h. Method provides enhancing the effectiveness of lymphostimulation and immunomodulation in cerebrospinal sclerosis. Invention can be used for lymphostimulation and immunomodulation in cerebrospinal sclerosis.
EFFECT: improved method for treatment.
1 tbl, 1 ex
SUBSTANCE: method involves introducing recombinant human granulocytic colony-stimulating factor.
EFFECT: enhanced effectiveness of treatment; increased number of stem cells and their improved homing in lesion foci without polypragmasia effects.