Medical tea with antiulcer activity


FIELD: pharmaceuticals, in particular tea for treatment and prophylaxis of gastric ulcer.

SUBSTANCE: claimed tea contains (wt. pts): calendula inflorescences 25; goosegrass leaves 10; cudweed grass 5; liquorices roots 10; elecampane rhizome 5; hawthorn fruits 15; dogrose fruits 10; sea-buckthorn fruits 5; coriander 5.

EFFECT: effective agent for treatment of gastric ulcer and other gastrointestinal diseases.

9 tbl, 3 ex

 

The invention relates to the field of medicine and for the establishment of fees for the treatment and prevention of gastric ulcer and duodenal ulcer.

When gastric ulcer and duodenal ulcer are widely used medicinal plants in the form of fees. Drug collection, consisting of grass cudweed herb (2 h), plantain (2 h), rosehips (1 h), chamomile flowers (2 h), yarrow (1 h) is used in the treatment of gastric ulcer in acute, chronic gastritis with high acidity (prototype). 10 g of the mixture pour 500 ml of boiling water, insist 5-6 hours, take 100 ml 3 times a day before meals [Krylov A.A. Marchenko A.V. Guide to herbal medicine. - SPb.: Publishing house"Peter", 2000. - S.].

Offer antimasonry collection (code name - "Entropic") compared to the prototype to a greater extent reduces the total acidity of gastric juice, increase the protective mucosal barrier of the stomach, prevents the formation of point hemorrhages and erosions in the gastric mucosa, a positive effect on digestive function of the stomach and duodenum, consists of the following medicinal plants in parts by weight:

Florets of calendula25
Ista plantain 10
Grass cudweed herb brown5
The roots of the licorice10
Roots of elecampane5
Hawthorn15
Hips10
The fruits of sea buckthorn5
Coriander fruit5

A specific example of obtaining the collection.

To prepare 1000 g of the proposed collection take florets of calendula crushed to particle size of 2.0 mm - 255 g; plantain leaves crushed to particle size of 2.0 mm 102 g; grass cudweed herb brown crushed to particle size of 2.0 mm - 51 g; the roots of licorice, crushed to a particle size of 2.0 mm 102 g; roots of elecampane, crushed to particle size of 2.0 mm - 51 g; hawthorn fruit, crushed to a particle size of 0.5 mm 153 g; rose hips crushed to particle size of 0.5 mm - 102 g; sea buckthorn crushed to particle size of 0.5 mm 51 g; coriander, crushed to particle size of 0.5 mm - 51, mix, sift vegetable dust through a sieve with apertures of 0.2 mm, and weighed. The loss of raw materials during grinding is 2%.

Part of the collection takes into account the contribution of biologically active substances (BAS) components collect in the treatment of ulcerative b is Lesni of the stomach and duodenum. It is generally accepted the use of herbs cudweed herb, plantain, licorice root, calendula blossoms, fruits of sea-buckthorn berries, coriander in the treatment of gastric ulcer and duodenal ulcer [Saakov, Ipomoeae. Handbook of medicinal herbs (Herbal medicine). - M.: Medicine, 1990. - 428 S.]. Ulcers in the stomach and duodenum using a separate group of compounds from plants, included in the proposed fee. The alantolactone - the amount of sesquiterpene lactones from the roots of elecampane has anti-inflammatory effect, reduces the proteolytic activity of gastric juice, enhances the formation of mucopolysaccharides in the gastric mucosa. 11, 13-digitaleconomy part of the molecule of sesquiterpene compounds included in the composition of the essential oils of the roots of Valerian makes the predominant contribution to the antiproliferative activity [.Konishi, Y.Shimada, T.Nagao et al. Antiproliferative sesquiterpene lactones from the roots of Inula helenium.// Biol. Pharm. Bull. - 2002. Vol.25, No 10. - P.1370-1372]. Plantasia - polysaccharide complex of plantain leaves has anti-inflammatory, antispasmodic, used in the treatment of gastric ulcer and duodenal ulcer with normal and low acidity. In the mechanism antiaging action of polysaccharides pentaglycine leading role played by incentive is the formation of slizeobrazovanie and strengthening of reparative processes in tissues of the stomach [Pronchenko G. Medicinal herbal remedies./ Edited Apersuasive, Iasmina. - M.: GEOTAR-MED, 2002. - 288 S.; Komissarenko, S.N., Spiridonov NR. Pectin - their properties and application.// Raising her. resources. - 1998. - T, issue 1. - S-119]. Ursolic acid is the most common in plants triterpene compound, inhibits inflammation, prevents the spread of plagues, inhibits the growth of cancer cells that has antiviral, immunomodulating properties [Pokrovsky A.G., Ilichev T.N., Pronyaev TR and other Immunomodulating activity of derivatives of ursolic acid.// Reports of the Academy of Sciences. - 1999. T. No. 3. - S-415].

The most effective ulcers flavonoids with a wide spectrum of pharmacological properties: antiyazvennaya, antispasmodic, anti-inflammatory, antioxidant, antimicrobial. The amount of flavonoids from calendula blossoms is the basis of kalapana - antiaging tools with anti-inflammatory and stimulates reparative processes properties. Antitone tool liquiritin consisting of the sum of flavonoids from licorice root has antispasmodic, anti-inflammatory, antacid action. Licorize is the main flavonoid licorice combined with quercetin and pectin are the basis antiaging means placarbil [Pronchenko G. Medicinal races is sustained fashion tools./ Ed. Apersuasive, I.A. Sumalinog. - M.: GEOTAR-MED, 2002. - 288 S.]. Flavonoids and other phenolic compounds (tannins, anthocyanins, phenolic acids play a key role in the regulation of free radical processes in gastric ulcer. The strengthening effect of phenolic antioxidants is achieved by substances synergists organic acids, free of carbohydrates, ascorbic acid, which regenerate the phenolic antioxidants, bringing radicals antioxidants from the scope of the oxidation reaction [Machacova GC, Orlov V.A. Nikolaev S.M. Pharmacological regulation of free radical processes in ulcers. - Ulan-Ude: Publishing house of the psybnc SB RAS, 2001. - 193 C.; Larson R.A. The antioxidants of higher plants.// Phytochemistry. - 1988. Vol.27, No 4. - P.969-978].

Thus, BAS constituent components of the proposed antiaging collection have the pharmacological properties required in the treatment of gastric ulcer and duodenal ulcer. They are dominated by the properties associated with the stimulation of production of protective mucus (polysaccharides plantain, elecampane, calendula and others), the strengthening of processes of regeneration (flavonoids calendula, cudweed herb, licorice, and others), reducing the inflammatory response (sesquiterpenoid elecampane, glycyrrhizin acid licorice, ursolic acid and other triterpenoids), regulatory Svobodnaya what's processes (water-soluble phenolic antioxidants, ascorbic acid and others).

Application in clinical conditions antiaging collection showed its high efficiency, is not inferior to the efficiency of the prototype - antiaging collection [Krylov A.A. Marchenko A.V. Guide to herbal medicine. - SPb.: Publishing house" Peter", 2000. - S]. Given in the text data of clinical and experimental tests allow to make a conclusion about the relevance of the proposed collection the criterion of "substantial differences", because the composition the proposed composition is different from the prototype, and proposed the formula of the invention the composition and ratio of ingredients of the proposed collection allow you to get positive results in the treatment and prevention of gastric ulcer and duodenal ulcer. To prove the reasonableness of the proposed composition of the collection the authors performed a series of experiments whose data are shown below.

The analyzed sample collection, made from a medicinal raw material, which is consistent with the current articles GF XI ed. [State Pharmacopoeia of the USSR: Issue 1. General methods of analysis. THE USSR MINISTRY OF HEALTH. - 11th ed., extra - M.: Medicine. - 1987. - 337 S.; V.2. General methods of analysis. Medicinal plant raw materials. THE USSR MINISTRY OF HEALTH. - 11th ed., extra - M.: Medicine. - 1990. - 400 C.]. Considering the fact that antimasonry effect of herbal medicines make a significant contribution of the flavonoids, triterpene compounds (glycyrrhizinate, ursolic acid), polysaccharides, defined their quantitative content of the proposed collection and broth (1:10). For the determination of BAS (mg/ml) broth (1:10) were prepared decoction of the proposed collection method GF X and XI ed. The yield of extractives in broth with chopped collection to particle size of 0.5 mm, 1.0 mm, 2.0 mm is not significantly different and is 35,80±0.11 mg/ml Decoction collection of the prototype was prepared according to the description [Krylov A.A. Marchenko A.V. Guide to herbal medicine. - SPb.: Publishing house "Peter", 2000. - S]: 10 g of the mixture was poured 500 ml of boiling water, insisted 5-6 hours the Yield of extractives in the broth of the prototype when it was 9.8±0.2 mg/ml Dry the rest of the broth (1:10) collect the prototype prepared by the method of GF XI ed. was 24.3±0.3 mg/ml

Quantitative determination of BAS in the collection and broth (1:10) was described in the literature methods: flavonoids, tannins, anthocyanins, organic acids, water-soluble polysaccharides according to the methods GF XI ed.; ascorbic acid - photocolorimetric method [Ehapest, Dmepos. Improving the technology of preparation and quality control of vitamin teas./ Actual problems of pharmaceutical technology: Scientific. proceedings of vniif. - 32. - M., 1997. - S-159]; triterpene saponins - spectrophotometry the definition method [Yourable, Way, Vraiement. Spectrophotometric method for quantitative determination of ursolic acid.// Chemistry of natural compounds. - 1972. No. 6. - S-740].

Data analysis to quantify the content of BAS artesina collection are shown in table 1.

3,11±0,05
Table 1.

The content of biologically active substances in artesina collection and broth (1:10)
NameThe content of BAS
in artesina collection, %in broth collection (1:10), mg/ml
Extractive substances extracted water44,89±0,2835,80±0,11
Flavonoids, rutin;0,92±0,010,53±0,01
Anthocyanins, in terms of cyanidin 3,5-diglucoside0,34±0,010,16±0,01
Ascorbic acid0,33±0,010,19±0,01
Water-soluble polysaccharidesthe 10.40±0,927,50±0,23
Tannins, in terms of tannin2,20±0,081,20±0,02
Organic acids, in terms of malic acid2,20±0,04
Triterpene saponins, in terms of ursolic acid9,79±0,01of 0.081±0,005

The content of flavonoids and tannins in broth collection of the prototype, prepared as described in [Krylov A.A. Marchenko A.V. Guide to herbal medicine. - SPb.: Publishing house "Peter", 2000. - S.], 0,26±0.01 mg/ml, 1,27±0.03 mg/ml, respectively; in broth, cooked GF XI ed., - 0,37±0.01 mg/ml and 1,94±0.03 mg/ml, respectively. The yield of extractives in the broth collection of the prototype, cooked GF XI ed., significantly higher than by way of Krylov A.A. (2000), and further experimental work decoction collection-prototype (drug comparison) was prepared according to the method of GF XI ed.

Examples of specific performance.

Example 1. Crushed to particle size of 2.0 mm florets of calendula 2.5 g; plantain leaves 1.0 g; grass cudweed herb swamp 0.5 g; the roots of the licorice 1.0 g; roots of elecampane 0.5 g; crushed to particle size of 0.5 mm hawthorn 1.5 g; wild rose 1.0 g; sea buckthorn 0.5 g; coriander 0.5 g mixed, placed in a flask with a capacity of 300 ml, add 100 ml of distilled water, the flask connected with a reflux condenser and heated on a water bath, maintaining the boil within 30 minutes After cooling for 10 minutes remove the & SMS filter through a paper filter, the obtained filtrate is used to determine the toxicity and pharmacological activity.

Determination of acute toxicity "Entropica conducted using the method of Cerberus [Manual on experimental (preclinical) study of new pharmacological substances. - M., 2000. - 678 C.]. It is established that the test collection "Entropic" after intragastric administration white rats to the greatest extent possible does not cause the death of laboratory animals for the entire observation period (14 days), which allows to relate it to the class of low-toxic substances according to the classification Via [Requirements for pre-clinical study of the General toxic action of new pharmacological substances. - M., 1984. - 49 S.].

The study of the antiulcer activity of Centrovita" was performed on experimental models "reflux-gastritis, which caused a 14-day intragastric introduction 1 per day medical bile white Wistar rats weighing 200 and 210 g of 10 ml/kg to the Animals of the experimental group 1 was injected "Entropic" in the form of decoction in a volume of 10 ml/kg after 6 hours after administration medical bile, 1 time per day throughout the experiment. As the comparison drug used collection prototype in the form of decoction, prepared according to the requirements of the global Fund XI ed., composed of: plantain (Lis the article), chamomile (flowers), cottonweed marsh (grass), yarrow (herb), rose hips (fruit) [Aka, V.A. Marchenko. Guide to herbal medicine. - SPb., 2000. - 416 S.]. A decoction of the collection of the prototype the animals of the experimental group 2 was injected on a similar scheme in their respective volumes. Animals of the control group was administered distilled water in an appropriate amount according to the same scheme. Studies were performed after 15 days from the beginning of the experiments.

To evaluate the antiulcer action of the proposed collection on the mucosa of the stomach of rats were counting the number of destruction, which was subdivided into small hemorrhages, erosions, and stripe-shaped ulcers. For each type of damage was calculated index Pauls" (PI) according to the formula [Animasola, Epishev, Thrain. The search for new anti-ulcer funds from plants of Siberia and the Far East// Expert. and clinically pharmacology. - 1998. - T 61. - P.31-35]:

,

where N is the average number of degradation in the group,

n % of animals with lesions in the group.

To assess acid and fermentopathia functions of the stomach was determined by the acidity of gastric juice by the method of Michaelis [Laboratory research methods /edited Riv. - M., 1987. - 365 C.], the content of pepsin in the gastric juice by the method Vintagelove [Vintahanohw. Modern methods of design, the national diagnose the condition of the gastric mucosa and their clinical significance. - M., 1965. - 212 C.]. The acid gastromucosal in the gastric juice was determined by the method J.Piper [J.Piper. The method of defininition of gastromucopolyglucosaes in the blood// J.Pharmacol. and Exp. Therap. - 1974. - Vol.52. - N.3. - P.42-45]. For the assessment of processes of lipid peroxidation and activity of antioxidant protection in the stomach homogenate and serum were determined by the content of malondialdehyde (MDA) and diene conjugates (DC) [Identally, Aggarwala. Method for the determination of malondialdehyde using thiobarbituric acid// Modern methods in biochemistry. - M., 1977. - P.66-68] and the level of catalase activity [Mahrous et al. Method for the determination of catalase activity// Laboratory work. - 1988. No. 6. - Page 16-19]. The obtained data statistically processed using the criterion of Mann-Whitney [Weathergirl, Ibbankatuwa. Mathematical statistics in clinical research. - M., 2001. - 256 S.]. The results of the experiments are shown in table 2.

It is established that "Entropic" against experimental "reflux-gastritis caused by the introduction of medical bile, has expressed an antiulcer action, preventing the development of destructive lesions of the stomach and helping to preserve its functional activity. In particular, the experimental animals treated with "Entropic" in the specified volume has decreased index Pulsars dot hemorrhages - 2.4 times; for erosion - more than 2 times compared with the data in the control, and the stripe-shaped ulcers in the appointment of the tested herbal remedies were not registered. On the background of a course introducing Entropica" there was an increase of acid and fermentopathia functions of the stomach: the content of free hydrochloric acid in gastric juice was increased by 38%, the rate of gastric secretion exceeded targets by 51%, debit hour hydrochloric acid was increased by 48%, the content of pepsin was increased 2.5 times, the flow-hour pepsin - 2.2 times compared with the data in animals of the control group.

Table 2

The influence of "Entropica" on the functional state of the stomach and indicators of lipid peroxidation and antioxidant defenses in white rats with experimental reflux-gastritis
IndicatorsGroups of animals
The intactControl (medical elixirs)Experimental 1 (medical bile+"Entropic")Experienced 2 (medical bile+drug comparison)
Free HCL, THE50,55±5,2020,61±2,8038,54±1,10*27,10±0,13*
General sour the face, THE144,40±1,3068,65±2,7291,52±3,24*89,13±6,01*
The infection rate. secretion, ml/100 g h0,50±0,060,35±0,020,53±0,04*0,42±0,01*
Debit hour HCL, mg/100 g h86,66±5,1138,06±4,7056,70±5,33*45,17±3,22
Pepsin (mg/ml39,91±3,249,54±0,6024,43±3,91*12,11±0,22
Debit hour pepsin, mg/h21,23±2,606,23±0,52of 13.58±1,41*9,04±0,18
Allenby blue in gastric juice, ug/ml345,1±30,5199,5±23,6284,4±18,7*215,6±20,0
Debit hour mucopolysaccharides, ug/h301,1±31,4303,2±31,5440,6±42,4*360,1±21,2
Index Pauls
tochek. Gravois.06,052,504,00
erosions00,500,210,31/td>
stripe-shaped ulcers00,3100,10
Catalase in the stomach homogenate, IU/g of tissue48,6±0,431,34±0,3345,10±0,34*36,45±2,05
MDA in the stomach homogenate, mmol/g tissue0,009±0,00010,34±0,0040,15±0,002*0,19±0,0001*
Note:*here and hereinafter, the difference being statistically significant compared to control at P<0,05.

Along with this, in the group of rats treated with the test collection "Entropic", the concentration of mucopolysaccharides exceeded targets by 45%. Course purpose of the test collection had a pronounced antioxidant effect, as evidenced by the inhibition of free radical oxidation of lipids and activation of antioxidant defenses. In particular, the content of malondialdehyde in the homogenate of the stomach wall was decreased by 56%, while the activity of catalase was increased to the level of performance in intact animals group. It was found that the antiulcer activity of the test collection "Entropica" a number of indicators (free hydrochloric acid, the rate of gastric secretion, the concentration of pepsin, flow-hour Monopol the saccharides, index Pauls for erosion and stripe-shaped ulcers, catalase activity) were significantly higher than the one used for the comparison drug.

Example 2. Crushed and sifted through a sieve with a diameter of 2.0 mm florets of calendula 2.5 g; plantain leaves 1.0 g; grass cudweed herb swamp 0.5 g; the roots of the licorice 1.0 g; roots of elecampane 0.5 g; crushed to particle size of 0.5 mm hawthorn 1.5 g; wild rose 1.0 g; sea buckthorn 0.5 g; coriander 0.5 g mixed, placed in a flask with a capacity of 300 ml, add 100 ml of distilled water, the flask connected with a reflux condenser and heated on a water bath, maintaining the boil within 30 minutes After cooling for 10 min extract filtered through a paper filter, the obtained filtrate is used to determine antiulcerogenic activity.

The study antiulcerogenic activity "Entropica" were performed on Wistar rats weighing 170-180 g using the model damage to the stomach, caused by the introduction of high doses of acetylsalicylic acid. Acetylsalicylic acid was administered intragastrically at a dose of 1000 mg/kg once for 3 hours prior to the introduction of the test herbal. Animals of the experimental group 1 was injected "Entropic" in the form of decoction in a volume of 10 ml/kg of prevention within 5 days 1 time per day and 3 hours after administration of acetylsalicylic acid as the comparison drug used above the gathering-prototype introduced animals from the experimental group 2 intragastric on a similar scheme in the corresponding volume. Animals of control group received distilled water in equianharmonic quantities in appropriate conditions. Studies were performed 24 hours after administration of aspirin. The data obtained are given in table 3.

Table 3

The influence of "Entropica" on morphometric characteristics of the gastric mucosa and indicators of lipid peroxidation and antioxidant protection in white rats with "aspirin" ulcer
IndicatorsGroups of animals
Control (aspirin)Experimental 1 (aspirin+"Entropic")Experienced 2 (aspirin+drug comparison)
Index Pauls

for point

bleeding

erosion

stripe-shaped ulcers


40,8


13,8


24,1
0,80,30,4
0,20,10,1
HMM, umol/ml min8,73±0,35 5,58±0,20*6,56±0,22
Catalase, mkat/l7,00±0,5210,99±1,05*8,15±0,35

As follows from the data in table 3, preventive introduction of Centrovita" with "aspirin-induced ulcer" had antiulcerogenic action, as evidenced by a significant decrease of the index Pauls in rats experimental group 1. So, in rats of this group index Pauls for dot hemorrhages were decreased 2.9 times for erosion - 2.6 times, for a stripe-shaped ulcers by 40% compared with the animals of the control group. It was also established that in rats fed the "Entropic", the content of malondialdehyde (MDA) in the serum of rats was decreased by 37%, while the catalase activity in the blood was increased by 57% compared with the corresponding data of the rats of the control group. It was found that such variables as the index Pauls and catalase activity, antiulcer activity "Entropica" somewhat superior to any in the comparison drug.

Antiulcer and anti-stress action of Centrovita" studied using a model of stress-induced damage to the stomach. Experiments conducted on rats male Wistar source weighing 150-160 g Immobilization stress induced by 24-hour immobilization of rats on the back. "Ve shall traffic" was introduced in the form of decoction in a volume of 10 ml/kg intragastrically prevention within 5 days 1 time per day and 1 hour before fixation animals. As the comparison drug used above the gathering prototype that was introduced in similar conditions in specific amounts. Studies were performed after 6 days from the beginning of the experiment. Determined ulcer index Pauls, the concentration of MDA, catalase activity by the methods described above, as well as the thymus, adrenal glands and spleen (in terms of 100 g weight of the animal).

The results of these studies are shown in table 4. As follows from the data in table 4, the course introduction "Entropica" against immobilization stress caused pronounced antiulcer action, as evidenced by the reduction of the index Pauls for dot hemorrhages 44%for erosion - more than 10 times and stripe-shaped ulcers by 47% compared with those of animals in the control group.

Table 4

Antiulcer, anti-stress and antioxidant action of Centrovita" in immobilization stress in rats
IndicatorsGroups of animals
The intactControl (stress)Experimental 1 (stress+"Entropic")Experienced 2 (stress+drug comparison)
Index Pauls

for point
hemorrhage022,20,212,3
erosions30,202,07,0
stripe-shaped ulcers17,13,2013,6
HMM, umol/ml min3,7±0,287,00±0,645.56mm±0,41*6,76±0,22
Catalase, mkat/l8,23±0,554,81±0,357,95±0,63*5,38±0,11
The thymus weight (mg/100 g153,5±7,21104,4±9,80135,6±the 10.40*119,6±7,10*
Weight of spleen (mg/100 g991,2±13,62594,4±25,05of 850.0±20,14*635,3±57,08
The weight of the adrenal glands, mg/100 g20,0±1,5431,1±1,0622,5±1,03*21,3±2,42*

The infusion of the test "Entropica" we also observed a decrease in the severity of stress-induced changes of internal organs: the weight of the adrenal glands of rats experimental group was 28% less than in control, and the weight of thymus and spleen matched with the public by 16% and 25% compared with the same indicators in animals of the control group. It was also established that "Entropic" exerted an antioxidant effect, as indicated by the decrease in the concentration of MDA in the serum of rats experimental group by 21% and increased the activity of catalase blood 1.7 times compared with the figures of animals in the control group.

Thus, the results of experimental studies indicate that "Entropic" has a strong antiulcerogenic action, protects the gastric mucosa in laboratory animals with the introduction of acetylsalicylic acid in high doses, prevents the development of atrophic gastritis after intragastric administration of bile (experimental reflux-gastritis") and has gastroprotective action in stress-induced damage to the stomach. While antiulcer activity of Centrovita were generally superior to that in the comparison drug.

Example 3. Crushed and sifted through a sieve with a diameter of 2 mm, florets of calendula 2.5 g; plantain leaves 1.0 g; grass cudweed herb swamp 0.5 g; the roots of the licorice 1.0 g; roots of elecampane 0.5 g; crushed to particle size of 0.5 mm hawthorn 1.5 g; wild rose 1.0 g; sea buckthorn 0.5 g; coriander 0.5 g mixed, placed in a flask with a capacity of 300 ml, add 100 ml of distilled water, the flask connected with a reflux condenser and naked is ewout in a water bath, supporting the boil within 30 minutes After cooling for 10 min, the extract was filtered through filter paper. The resulting broth was used for clinical studies.

Clinical study of the antiulcer action of Centrovita" conducted on the basis of clinical inpatient and outpatient departments of the Centre of Oriental medicine of the Ministry of health of the Republic of Buryatia (MH RB). Examined 48 patients with disease of the stomach and duodenum, which had been under observation in the period from November to December 2005, All patients randomly as admission to hospital were divided into groups: 1st group - 10 people (control) - patients who were prescribed conventional complex therapy (antacids, anticholinergic drugs gastrotsepin, H2-receptor blockers histamine - ranitidine, famotidine, and others). The 2nd group of patients (28 men) were appointed "Entropic" in the form of decoction, prepared according to the requirements of GF (XI) 1/3 Cup 3 times a day 30 minutes before a meal. The 3rd group of patients (10 people) were prescribed the drug comparison - broth collection of the prototype, which consists of: plantain (leaf), chamomile (flowers), cottonweed marsh (grass), yarrow (herb), rose hips (fruit) [Aka, V.A. Marchenko Guide phyto is therapy. - SPb., 2000. - 416 S.]. Drug comparison was prepared in the form of decoction, prepared according to the requirements of GF (XI) and was appointed on a similar scheme in their respective volumes. The duration of treatment was 15-21 day.

Clinical characteristics of patients are given in table 5. A significant number of patients (52.1 per cent) were aged from 42 to 50 years. This clinical study recommended herbal remedies are presented separately for each group. Thus, the group of patients with chronic gastritis with decreased secretion was 3 patients (6.3%)and 5 patients with erosive gastritis (10.4%), and 22 people suffered from stomach ulcers (45.8%) and a group of 18 people (37,5%) consisted of patients with peptic ulcer of the duodenum.

Table 5

Clinical characteristics of the patients
Distribution of patients according to parametersThe number of patients, anti-lock brakes. (%)
I. Age, years
42-5025 (52,1)
51-5510 (20,8)
55-6113 (27,1)
Total:48 (100)
II. Nosological forms:
Gastritis with decreased secretion3 (6,3)
Erosive gastritis5 (10,4)
Peptic ulcer22 (45,8)
Peptic ulcer disease duodenal ulcerand 18 (37.5)
Only48 (100)

As the number of patients with chronic gastritis were under fading aggravation, the criteria for assessing the effectiveness of actions "Entropica" served as the dynamics of clinical symptoms (disappearance of pain, eructation, normalization of stool, appetite, peripheral blood, the results of instrumental studies. In 84% of patients hypoacid gastritis on the background of a course assignment test Fiorella noted the disappearance of pain or a reduction in their frequency and intensity in the epigastric region, was painless palpation, more than 50% of the patients disappeared belching, early admission "Entropica" the overwhelming number of patients recovered appetite, improve the function of intestines. Signs of positive dynamics in the majority of patients appeared already at the end of the first week of taking the herbal (table 6). When a drug comparison of these positive changes in the development hypoacidic gastritis were less pronounced.

Table 6

To Inocencia criteria pharmacotherapeutic efficiency antiaging of herbal in patients with chronic hypoacid gastritis
Clinical symptoms ofThe control group (7 days)The control group (15 days)When assigning "Entropica"When a drug comparison
After 7 days.After 15 days.After 7 days.After 15 days.
Pain in the epigastric region86 (25%)254 (84%)96 (34%)
Palpation tenderness in the epigastric region75 (29%)206 (70%)74 (43%)
Burp107211184
Reduction in the rate of secretion of gastric63 (50%)225 (78%)73 (58%)
Disorder of appetite106 (40%)235 (79%)63 (50%)

Evaluation of clinical and instrumental data, and secretory activity of the stomach in the dynamics showed that after the appointment of "Entropica" the volume of gastric juice increased the I 44,4± 1.1 to 60,0±1.2 ml, debit hour of hydrochloric acid with a 2.0±0.2 to 3,4±0.5 mEq/h, flow-hour pepsin - 0,55±0.01 to 1.2±0.1 g/H. the Increase acid-forming and fermentopathia functions of the stomach after the appointment of the specified herbal, obviously, is associated with improved functional status of the entire glandular apparatus of the gastric mucosa.

The purpose of Centrovita" sick erosive gastritis caused the vast majority of patients to the disappearance of pain in the epigastric region and the beginning of scarring ulcers by the end of the first week after receiving the specified herbal. By the end of the course of treatment (15 days) improved the General condition of patients, most of the clinical symptoms of erosive gastritis practically not observed (epigastric pain, belching, disturbance of appetite). During visual examination of the gastric mucosa (FGS) in 4 patients out of 5 (80%) were reported complete healing of erosive defects of the mucous membrane of the stomach. In particular, the observed normalization of the relief of the mucous membrane of the stomach, decreased the severity of edema and hyperemia were no signs of irritation, decreased the number of mucus, and areas where previously discovered erosive changes, after taking a course of the tested herbal practically did not differ from intact areas was slit the stand of the lining of the stomach. By the end of the course receive "Entropica" (after 21 days) in 100% of patients had almost complete healing of erosions. The study of the rate of gastric secretion revealed increase it 3 of 5 patients, on average, 31%, which showed improvement of the functional activity of the stomach.

Peptic ulcer studies have been reported in 22 patients, peptic ulcer disease duodenal ulcer - in 18 patients. The disease in all patients was uncomplicated. In 25% of patients the diagnosis was established for the first time, 12% of patients with ulcer history had from 1 to 5 years 5 to 10 years - 35%, more than 10 years in 28% of patients; 38% of patients reported long-term experience of Smoking, and 10% reported frequent use of alcohol. The size of ulcers of the mucous membranes ranged from 0.5 to 1.7 cm in diameter. Moderately expressed inflammation of the mucous membrane were recorded in 42.1% of patients, expressed in 33.3%, blown - in 24.6% of cases. All patients were divided into 3 groups depending on the treatment (table 7).

Table 7

The main clinical manifestations of gastric ulcer and duodenal ulcer (%)
Symptomsgroup 1 (standard therapy)group 2 ("Entropic")group 3 (repeat comparison)
Daily pain76,568,577,1
Night pain87,688,491,5
Heartburn89,486,377,5
Nausea34,541,133,6
Burp99,087,679,4
Flatulence32,340,035,6
Constipation66,5to 58.144,2
Diarrhea8,76,610,0

group 1 patients received famotidine (20 mg 2 times a day) or omeprazole (20 mg 2 times a day); group 2 - "Entropic" in the form of broth 1/3 Cup 3 times a day 30 minutes before meals; group 3 - drug comparisons in the form of decoction collection of the prototype in a similar situation in equianharmonic quantities. The group of patients on the basic parameters and clinical manifestations of the disease had no significant differences (table 8).

In the analysis of symptoms of peptic ulcer revealed that when the appointment of a "Entropica patients by the end of the first week of treatment disappeared pain in the epigastric region, heartburn, belching, flatulence, dyspepsia (table 8).

Table 8

The symptoms of peptic ulcer in exchange appointment antiaging of herbal (%)
Symptomsgroup 1 (standard therapy)group 2 ("Entropic")group 3 (drug comparison)
Daily pain68,989,077,5
Night pain65,691,072,2
Heartburn87,998,586,3
Nausea88,686,5of 87.0
Burp79,585,580,4
Flatulence46,756,248,7
Constipation35,3of 56.4of 45.7
Diarrhea43,378,968,6

Endoscopic control of peptic ulcer disease (EGD) showed that in the group of patients who received "Entropic", noted the localization of the inflammatory process after 14-15 days of observation, possible scarring ulcers were shortened up to 12-15 days ulcers, duodenal ulcers and up to 10-12 days with a stomach ulcer. It is noted the formation of hyaline cartilage scar without deformation of clisis the second shell. The healing of the ulcer we re-examined patients with peptic ulcer pointed to a noticeable therapeutic effect of this tool. Patients showed activation of reparative processes, reflected in the reduction of puffiness surrounding the ulcer of the mucous membrane and the cleansing of the bottom of the ulcer crater.

Was studied the dynamics of processes of lipid peroxidation and antioxidant defense in patients with peptic ulcer disease with the purpose of Centrovita", from the acute stage and the finishing stage of sustained remission. All patients were divided into 4 groups: group 1 - the acute phase of the disease; group 2 - phase scarring; group 3 - phase unstable remission; group 4 - phase stable remission. Division of the patients in the above groups was carried out according to endoscopic control with state estimation of the mucous membrane of the stomach and duodenum. Additionally, the group was formed from 10 healthy volunteers. In the serum was determined by the content of the primary (diene conjugates - DK) and secondary (malonic dialdehyde - MDA) products perioxidase lipids and the activity of catalase. The results are shown in table 9.

Table 9

The influence of "Intropia is on the processes of lipid peroxidation in gastric ulcer and duodenal ulcer
Phase of development of gastric ulcer and duodenal ulcerIndicators
MDA, nmol/mlDK, nmol/mlCatalase, µm/mg
Healthy volunteers36,40±1,2427,14±0,22668,0±25,1
Acute phase66,21±4,1235,99±2,15387,89±14,50
Phase scarring
"Entropic"48,33±2,1254,81±0,98389,3±14,2
Drug comparison34,44±3,13*48,92±3,85*584,9±41,9*
Phase unstable remission
Drug comparison45,76±2,1552,16±4,23546,2±34,1
"Entropic"35,12±2,11*34,19±1,17*675,5±23,7*
Phase stable remission
Drug comparison39,16±3,0133,11±3,15641,1&x000B1; 48,2
"Entropic"29,30±1,05*is 25.50±1,18*666,8±and 23.4
Note:*the difference is significant in comparison with patients treated with the drug comparisons at P<0,05

As follows from the data shown in this table, the content of MDA in the serum of patients with gastric ulcer and duodenal ulcer in exchange destination of Centrovita" decreased in phase cicatrization of the ulcer by 29%DK 11%; in the phase of unstable remission at 24 and 35%, respectively, compared with patients receiving comparator drug.

Along with this, in exchange destination of Centrovita" the catalase activity of blood serum was increased in phase scarring 50%, in the phase of unstable remission by 24% compared with those patients treated with the decoction collection of the prototype. At the same time, it should be noted that the tested herbal remedy "Entropic" significantly reduced the intensity of free radical reactions and increased the activity of antioxidant enzyme catalase in comparison with the indices in patients in the acute phase of peptic ulcer, which provides, apparently, antiulcerogenic action "Entropica".

Thus, taking into account decline the definition and clinical studies "Entropica installed it pronounced therapeutic effect in the target organ of gastroduodenal zone, which is provided through a wide range of pharmacological properties of biologically active substances contained in the proposed drug collection.

Drug collection with intesinal activity based on vegetable raw materials, plantain leaves, grass cudweed herb, rosehips, characterized in that the quality of plant raw material further comprises florets of calendula, licorice roots bare, elecampane rhizome, hawthorn, sea-buckthorn berries, coriander fruit, parts:

Florets of calendula25
Plantain leaves10
Grass cudweed herb brown5
The roots of the licorice10
Roots of elecampane5
Hawthorn15
Hips10
The fruits of sea buckthorn5
Coriander fruit5



 

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3 ex

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11 cl, 2 tbl

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7 ex

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10 cl, 8 ex

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5 cl, 4 ex

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5 cl, 4 ex

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EFFECT: drugs with enhanced pharmaceutical action and high therapeutic activity in decreased dose; simplified method of high yield; bioactive substances of increased stability.

5 cl, 4 ex

FIELD: pharmaceutical industry, in particular production of pharmaceutical agent having sedative action.

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4 ex

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EFFECT: new matters for cosmetic products.

1 ex

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EFFECT: non-toxic and effective agent for treatment of abovementioned diseases.

7 ex

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