Method for production of hepatothropic artichoke extract-based polyphenol pharmaceutical agent

FIELD: pharmaceutical industry, in particular production of hepatothropic drug based on artichoke polyphenols.

SUBSTANCE: claimed method includes breakage of fresh artichoke leaves, treatment with hot steam for certain time; two-step extraction with hot water under specific conditions, extract separation from solvent cake, concentration of artichoke aqueous extract in vacuum to produce syrup-like extract. Obtained syrup-like extract is blended with filler, detacher and lubricant in specific component ratio.

EFFECT: method of increased yield; biologically active substances of improved stability; reduced raw material and extractant consumption; capsulated hepatothropic drug with high therapeutic activity and prolonged storage time.

10 cl, 8 ex

 

The invention relates to medicine and pharmaceutical industry, in particular to methods for production of medicines, with hepatotropic action on the basis of polyphenols artichoke.

A method of obtaining polyphenols from fresh leaves of the artichoke, which is the basis of the medicinal product with hepatotropic action, in particular choleretic and hypoazotemic action, including extraction, cleaning, washing, evaporation, drying under vacuum [1]. The extraction was carried out by boiling the extractant, taken in the ratio of raw material to the extractant is 1:5. As a solvent using n-butyl alcohol. Spend cleaning liquid extract by adding to the cooled solution of equal volume of heptane, washing with water and evaporation of the organic mixture to the dry residue. The obtained residue for separation of the target product, which is the basis of the medicinal product, dissolved in methanol and add a five-fold volume of diethyl ether. Then dropped the precipitate was separated by centrifugation, washed with ether and dried under vacuum over phosphorus pentoxide. The result is 2% of the weight of fresh raw materials with the content of biologically active substances in the final product 14-16%. However, this method has drawbacks due to the complexity of the technological process, the light is Anna with the complexity of the stages of extraction and purification, which uses a significant amount of flammable and toxic solvents used as extractants, which has a negative impact on process safety. In addition, the process is time-consuming and expensive. It should also be noted that the use in the production of alcohols limits the number of consumers of a medicinal product derived from polyphenols artichoke, since the drug may not apply to patients suffering from such diseases as diabetes and patients suffering from cardiovascular diseases.

The closest to a method for drug hepatotropic funds on the basis of polyphenols is a method for polyphenols from fresh leaves of the artichoke, which is the basis of the medicinal product, which includes grinding the fresh leaves of the artichoke, the extraction of boiling water in two stages, the concentration of an aqueous extract of artichoke under vacuum to a syrup-like state of extract, filtering the extract [2]. As extractant in the process use water, extraction was carried out until the amount of extract equal 1-2:10 by weight of the raw materials, carry out the cleaning liquid extract solution 96-98%ethyl alcohol, taken in the ratio of 1.1 to 0.9:0,9-1,1, carry out the division is their sludge, is acidified to a pH of 2-3 is obtained the product, treat it with a mixture of isobutyl alcohol and ethyl acetate, taken in the ratio from 1:6 to 6:4. This method is more secure than previous similar because they use fewer flammable and toxic substances, which also simplifies the process. In addition, as an extractant use water, which reduces the cost of the process. Thanks to the clean gain greater yield of biologically active substances in the final product. However, the method is still expensive, time-consuming and laborious, as in the process using different alcohols and many stages of preparation. Furthermore, the resulting dried extract has a high hygroscopicity, which reduces its shelf life. Derived from polyphenols drug also has limitations on the application due to the use of alcohols in the production process.

The basis of the invention the task is to provide such a method of obtaining drug hepatotropic funds on the basis of polyphenols artichoke, which by the selection of technological operations in a certain sequence, modes and parameters, as well as due to selection of components of auxiliary substances, taken in a certain ratio, achieved high wychodzilismy active substances and medicinal product stability, having a high therapeutic effect, without contraindications for patients with diabetes and patients suffering from cardiovascular diseases, advanced contingent of consumers, increased retention while reducing consumption of raw materials and extractive solvent and the reduction of material costs and simplifying the manufacturing process.

The problem is solved in that in the known method of obtaining drug hepatotropic funds on the basis of polyphenols, which includes grinding plant materials fresh leaves of the artichoke, the extraction of vegetable raw materials in the presence of the extractant, such as boiling water, the concentration of an aqueous extract of artichoke in a vacuum until syrupy state extract, filtering the extract, re-extraction of vegetable raw materials in the form of meal, according to the utility model extraction is carried out at the ratio of raw material and extractant 1:(7-9), the concentration conduct to amount to 1/70-1/90 of the original volume of the extractant, and the resulting syrupy extract mixed with a filler, the baking powder and the moving substance in the following ratio, wt.%:

Extract25-40,5
Baking powder 5-15
Moving substance0,5-5
FillerRest

Preferably take plant material grown in the area with a temperate climate.

In addition, take plant material, cut no more than three days before processing.

It is advisable before extraction the leaves of the artichoke pre-steamed hot steam within 20-40 minutes.

In addition, the conduct extraction with purified water.

Preferably after each stage extraction to infusion, and after the first stage insisting conduct within 170-190 minutes, and after the second stage within 110-130 minutes.

Suitable concentration of an aqueous extract of artichoke carried out in vacuum at a pressure vacuum 0,070-0,080 MPa and steam pressure 0,20-0,30 MPa.

Preferably as a filler to take microcrystalline cellulose.

In addition, as baking powder to take Aerosil or silicon dioxide.

It is advisable as a moving substance to take stearic acid and its salts.

Preferably obtained the drug to be capsulerebel.

To obtain medicinal hepatotropic funds on the basis of polyphenols artichoke use extraction fresh leaves of the artichoke, the cat heaven allows to obtain a drug with a high content of biologically active substances and has a therapeutic effect. The biological activity of the extract of artichoke mainly due to the presence of polyphenolic compounds, the main of which are tsinarin, chlorogenic and caffeic acid. Due to the complex of these compounds artichoke extract stimulates bile secretion, it has a detoxifying effect on the liver parenchyma, has a positive effect on lipid metabolism, promotes the release of toxins from the body and normalize metabolism.

Extraction of the leaves of the artichoke can be made in various ways, for example by way of maceration (soaking), percolation (transmission through the leaves of the artichoke continuous stream of extractant), countercurrent extraction, ultrasonic extraction, extraction on a rotary pulsation apparatus (extraction takes place simultaneously with the grinding of raw materials), cavitation method, extraction with hot water with constant stirring.

As extractant can be used various alcohols, spirtovanie mixture of water and organic solvents. The choice of solvent depends on the type of biologically active substances, which must be extracted from plant materials.

As extractant in the present method selected water that extracts from the leaves of the artichoke water-soluble polyphenols with hepatotropic properties. It should be noted, is that when this treated water is used, that provides native characteristics of bacterial and safety of biologically active substances, and also reduces the concentration of salts and other impurities in the extract and increases the purity of the finished product. The use of water reducing material costs while receiving drug compared to alcohols. In addition, the use of this extractant widen the number of consumers of the drug, since the drug can be applied patients suffering from such diseases as diabetes and patients suffering from cardiovascular diseases.

Of great importance in the extraction process is the selection of the ratio of raw material and extractant. Experimentally obtained optimal ratio of raw material and extractant, which is 1: (7-9), which gives a more complete extraction of biologically active substances. When the ratio of 1:6 and less than the extract yield is reduced and less extracted biologically active substances. To obtain the required number of biologically active substances is required to increase the consumption of plant material, which is impractical. With a ratio of 1:10 or more increases the volume of liquid extract, which requires additional time and resources to further the process of obtaining drugs is.

The concentration of the extract under vacuum allows to obtain a syrupy artichoke extract with a high content of biologically active substances. When it is determined experimentally that the concentration of appropriate conduct to amount to 1/70-1/90 of the original volume of the extractant. With this ratio in the extract remain biologically active substances, and is the amount of moisture that is optimal for further medication. In addition, the receipt of artichoke extract in a syrupy condition reduces process, eliminating the drying process, which in turn ensures the safety of biologically active substances, reducing material costs for production and simplifies the process. In addition, the dry extract of artichoke is a hygroscopic substance. When storing it absorbs moisture, which leads to clumping and sticking together of the extract and reduce its shelf life.

Preliminary steaming hot steam the artichoke leaves before extraction allows to suspend the enzymatic processes in raw and save biologically active substances, as well as reduce the amount of raw material and thus reduces the amount of extractant.

Insisting after each stage of EXT is aerovane also allows you to extract more biologically active substances from raw materials.

Growing vegetable raw - artichoke in the area with a temperate climate allows to increase the yield of green mass of leaves of the artichoke, and processing of plant materials fresh for not more than three days after cutting makes it possible to retain biologically active substances in the original plant material.

In order to use artichoke extract as a medicine, it is mixed with auxiliary agents such as a filler, a leavening agent, a sliding agent and capsulebuy.

As filler take microcrystalline cellulose (MCC), which is a binder component in the composition of the medicinal product, as well as enhances the action of the main biologically active substances and has absorbent properties. The optimal number of cells selected in the range 44-65 wt.%.

Used as baking powder Aerosil or silicon dioxide provide flowability of the mixture at packing into capsules and contribute to the fact that inside the capsule creates a loose structure that has a positive effect on the absorption of encapsulated drug by the body. The optimum content of the powder is selected in the range of 5-15 wt.%. In addition, this powder also has absorptive properties, reduces the hygroscopicity ready Lekarstvo the th means, which increases its shelf life.

Stearic acid or its salts are used as the moving substance in order to give the mixture a sliding effect, which makes it easier to fall asleep in her capsule. The optimum quantity of such substance selected in the range of 0.5-5 wt.%.

In addition, the use of auxiliary substances, not containing sugar, not only allows you to create easy to use dosage form in the form of capsules, but also to expand the indications for medical use of the drug because this drug can be administered to patients with diabetes mellitus.

It should be noted that this method is cheaper, because the process does not use alcohol on any of the stages of preparation of medicines. In addition, the exclusion of alcohol at all stages of the process of obtaining medicines are also expands the indications for medical use, resulting in the drug can be taken in different patients of different age groups, including patients suffering from diseases of the cardiovascular system.

Received drug capsulebuy, resulting receive the drug in a convenient dosage form, resulting in a higher bioavailability and maintain the activity of biologically active substances during storage.

Thus, the method of obtaining drug hepatotropic funds on the basis of polyphenols provides obtaining of a drug with a high therapeutic efficiency, high output and stability of biologically active substances by increasing shelf life of medicines and reducing consumption of raw materials and extractive solvent, which reduces material costs and simplifies the process.

The claimed invention is as follows.

Example 1.

Fresh grass artichoke in the amount of 100,00 kg crushed on cormorant type I-KU, placed in the extractor and are steamed hot steam for 25 minutes. Then the extractor serves purified hot water in the amount of 350 l, bring to the boil and boiled by hot steam in the jacket extractor for 25 minutes. After that, the boiling stops and the extract was infused for 175 minutes. After infusion of the extract is filtered and the filtered extract is poured into the measuring device (first discharge). Then in the extractor with the raw materials again pour hot water in the amount of 350 l and boiled again by blowing hot steam in the jacket extractor for 25 minutes. After boiling the extract infused for 115 minutes. Then the extract was filtered and the filtered extract is poured into the same measuring device, the first SL the century Liquid extract is served in a vacuum evaporation apparatus and spend the concentration of the extract by evaporation at a pressure rarefaction of 0.075 MPa and the vapor pressure of 0.20 MPa to reduce the amount of extractant 80 times. The density of the extract after concentration is not less than 1.30 grams/cm2. Then take Aerosil and microcrystalline cellulose, taken in an amount 1,736 kg each, and mix to a uniform distribution of aerosol in the mixture. The resulting syrupy extract is mixed with the mixture and microcrystalline cellulose, taken in an amount 32,614 kg. Mixture is stirred in the mixer-granulator to obtain a homogeneous composition within 10 minutes. The mixture is then granularit through the mesh hole diameter 3 mm, the Obtained granulate is gravity fed to the baking sheets and spread a thin uniform layer of the same thickness, the trays are placed in a heat chamber and dried at a temperature of 70°With inlet and 50°With output. Residual moisture in the granules is not more than 3.0%. Then the granules optivault the stearate of calcium taken in the amount of 0,450 kg, universal mill. After that, the mixture was fed to a machine for filling capsules model Zanasi 40 F and capsulebuy. Get capsules weighing 0.3 g, containing 0.1 g of artichoke extract (in terms of dry matter) and auxiliary washes the VA, including filler is microcrystalline cellulose 0,1880 g baking powder - Aerosil 0,0095 g and moving substances - calcium stearate 0,0025,

Example 2.

Fresh grass artichoke in the amount of 100,00 kg crushed on cormorant type I-KU, placed in the extractor and are steamed hot steam for 25 minutes. Then the extractor serves purified hot water in the amount of 450 litres, bring to the boil and boiled by hot steam in the jacket extractor for 40 minutes. After that, the boiling stops and the extract was infused for 190 minutes. After infusion of the extract is filtered and the filtered extract is poured into the measuring device (first discharge). Then in the extractor with the raw materials again pour hot water in the amount of 450 litres and again boiled by hot steam in the jacket extractor for 40 minutes. After boiling the extract infused for 130 minutes. Then the extract was filtered and the filtered extract is poured into the same measuring device, and the first drain. Liquid extract is served in a vacuum evaporation apparatus and spend the concentration of the extract by evaporation at a pressure vacuum 0,080 MPa and the vapor pressure of 0.30 MPa to reduce the volume of the extractant in 90 times. The density of the extract after concentration is not less than 1.30 grams/cm2. Then take Aerosil and microcrystalline cellulose, taken in Koli is este 8,21 kg each, and stirred until a uniform distribution of aerosol in the mixture. The resulting syrupy extract is mixed with the mixture and microcrystalline cellulose, taken in an amount 15,97 kg. Mixture is stirred in the mixer-granulator to obtain a homogeneous composition within 10 minutes. The mixture is then granularit through the mesh hole diameter 3 mm, the Obtained granulate is gravity fed to the baking sheets and spread a thin uniform layer of the same thickness, the trays are placed in a heat chamber and dried at a temperature at a temperature of 70°With inlet and 50°With output. Residual moisture in the granules is not more than 3.0%. Then the granules optivault the stearate of calcium taken in the amount of 0,281 kg, universal mill. After that, the mixture was fed to a machine for filling capsules model Zanasi 40 F and capsulebuy. Get capsules weight of 0.3 g containing 0,121 g of artichoke extract (in terms of dry matter) and excipients, including filler is microcrystalline cellulose 0,1320 g baking powder - Aerosil 0,045 g and moving substance - stearate 0,0015,

Example 3.

Fresh grass artichoke in the amount of 100,00 kg crushed on cormorant type I-KU, placed in the extractor and are steamed hot steam for 25 minutes. Then the extractor serves purified hot water in quantity is as 400 l, bring water to a boil and boiled by hot steam in the jacket extractor for 30 minutes. After that, the boiling stops and the extract was infused for 180 minutes. After infusion of the extract is filtered and the filtered extract is poured into the measuring device (first discharge). Then in the extractor with the raw materials again pour hot water in the amount of 400 l and over again boiled by hot steam in the jacket extractor for 30 minutes. After boiling the extract infused for 110 minutes. Then the extract was filtered and the filtered extract is poured into the same measuring device, and the first drain. Liquid extract is served in a vacuum evaporation apparatus and spend the concentration of the extract by evaporation at a pressure vacuum 0,070 MPa and the vapor pressure of 0.25 MPa to reduce the amount of extractant 70 times. The density of the extract after concentration is not less than 1.30 grams/cm2. Then take silicon dioxide and microcrystalline cellulose, taken in an amount 2,74 kg each, and mix to a uniform distribution of silicon dioxide in the mixture. The resulting syrupy extract is mixed with the mixture and microcrystalline cellulose, taken in an amount 32,88 kg. Mixture is stirred in the mixer-granulator to obtain a homogeneous composition within 12 minutes. The mixture is then granularit through Seth is the hole diameter 3 mm The obtained granulate is gravity fed to the baking sheets and spread a thin uniform layer of the same thickness, the trays are placed in a heat chamber and dried at a temperature at a temperature of 75°With inlet and 55°With output. Residual moisture in the granules is not more than 3.0%. Then the granules optivault the stearate of calcium taken in the amount of 2.81 kg, universal mill. After that, the mixture was fed to a machine for filling capsules model Zanasi 40 F and capsulebuy. Get capsules weighing 0.3 g, containing 0.075 g of artichoke extract (in terms of dry matter) and excipients, including filler is microcrystalline cellulose of € 0.195 g, powder of silicon dioxide 0,0150 g and moving substance is stearic acid 0,0150,

Example 4. Extract the density of 1.30 g/cm2get as described in Example 1. Then take polyplasdone and starch, taken in an amount 2,71 kg each, and mix to a uniform distribution polyplasdone in the mixture. The resulting syrupy extract is mixed with the mixture and starch, taken in an amount 29.99 is kg of the resulting mixture is stirred, granularit, dried, optivault stearyl sodium fumarate in the number of 3.04 kg and capsulebuy as described in Example 1. Get capsules weighing 0.3 g, containing 0.1 g of artichoke extract (in terms of dry substances is about) and excipients: starch - filler, polyplasdone - baking powder and stearyl sodium fumarate is moving in an amount of about 0.17 g, 0,012 0,018 g and g, respectively.

Example 5. Extract the density of 1.30 g/cm2get as described in Example 1. Then take crosspovidone and calcium diphosphate, taken in an amount 8,31 kg each, and mix to a uniform distribution polyplasdone in the mixture. The resulting syrupy extract is mixed with the mixture and calcium diphosphate, taken in an amount 17,87 kg of the resulting mixture is stirred, granularit, dried, optivault stearyl sodium fumarate in the number of 1.38 kg and capsulebuy as described in Example 1. Get capsules weighing 0.3 g, containing 0.1 g of artichoke extract (in terms of dry matter) and excipients: calcium diphosphate - filler, crospovidon - baking powder and stearyl sodium fumarate is moving in an amount of about 0.15 g, 0.008 g and 0,042 g, respectively.

Example 6. Extract the density of 1.30 g/cm2get as described in Example 1. Then take sodium glycolate, and starch, taken in an amount of 8.4 kg each, and mix to a uniform distribution of sodium glycolate in the mixture. The resulting syrupy extract is mixed with the mixture and starch, taken in an amount 25,13 kg of the resulting mixture is stirred, granularit, dried, Opadry is with calcium stearate in the amount of 0.62 kg and capsulebuy as described in Example 1. Get capsules weighing 0.3 g, containing 0.1 g of artichoke extract (in terms of dry matter) and excipients: starch - filler, sodium glycolate - baking powder and calcium stearate is moving in an amount of about 0.16 g, 0.037 g and 0.003 g, respectively.

Example 7. Extract the density of 1.30 g/cm2get as described in Example 1. Then take Aerosil and microcrystalline cellulose, taken in the amount of 4.5 kg each, and mix to a uniform distribution of sodium glycolate in the mixture. The resulting syrupy extract is mixed with the mixture and microcrystalline cellulose, taken in an amount 30,44 kg of the resulting mixture is stirred, granularit, dried, optivault stearyl sodium fumarate in the number of 2.81 kg and capsulebuy as described in Example 1. Get capsules weighing 0.3 g, containing 0.1 g of artichoke extract (in terms of dry matter) and excipients: microcrystalline cellulose - filler, Aerosil - baking powder and stearyl sodium fumarate is moving in an amount of about 0,BG, 0,025 0,016 g and g, respectively.

Example 8. Extract the density of 1.30 g/cm2get as described in Example 1. Then take Aerosil and starch, taken in an amount of 5.1 kg each, and mix to a uniform distribution of sodium glycolate in the mixture. After this is obtained syrupy extract is mixed with the mixture and starch, taken in quantity 21,31 kg of the resulting mixture is stirred, granularit, dried, optivault calcium stearate in the amount 3,49 kg and capsulebuy as described in Example 1. Get capsules weighing 0.3 g, containing 0.1 g of artichoke extract (in terms of dry matter) and excipients: starch - filler, Aerosil - baking powder and calcium stearate is moving in an amount of about 0,148, 0,028 g and 0,019 g, respectively.

Obtained the drug of plant origin on the basis of polyphenols artichoke exerts choleretic, diuretic, hepatoprotective and hypolipidemic effects, increases bile secretion, promotes the secretion from the body of nitrogen-containing substances (urea, creatinine), toxins, and reduces the total cholesterol in the blood. Present in the extract of artichoke, ascorbic acid, carotene, vitamin B1and In2insulin helps to normalize metabolism. In addition, due to the fact that in the process of getting medication not used sugar-containing auxiliary substances and alcohol, the drug can be taken in different patients of different age groups, including patients with diabetes, patients suffering from diseases of the cardiovascular system.

The claimed method allows to obtain l the drug means hepatotropic actions no contraindications for patients with diabetes and patients suffering from cardiovascular disease, and increases the shelf life of the medicinal product, the reduction of material costs for the production of medicines

Sources of information

1. French patent No. 2071133, publ. 17.09.1971,, IPC C 07G 3/00.

2. USSR author's certificate No. 644771, publ. 30.01.1979,, IPC C 07 C 37/28, a 61 K 35/78.

1. The method of obtaining drug hepatotropic funds on the basis of polyphenols, which includes grinding plant materials fresh leaves of the artichoke, the extraction of boiling water in two stages, the separation of extracts from the meal, combining the obtained extracts, the concentration of an aqueous extract of artichoke in a vacuum until syrupy state extract, characterized in that before extraction the leaves of the artichoke predvaritelno are steamed hot steam within 20-40 min, the extraction is carried out at a ratio of raw material and extractant 1:(7-9), the concentration conduct to amount to 1/70-1/90 of the original volume of the extractant, and the resulting syrupy extract mixed with a filler, the baking powder and the moving substance in the following ratio, wt.%:

Ek is a path 25-40,5
Baking powder5-15
Moving substance0,5-5
FillerRest

2. The method according to claim 1, characterized in that take plant material grown in the area with a temperate climate.

3. The method according to claim 1, characterized in that take plant material, cut no more than three days before processing.

4. The method according to claim 1, characterized in that the extraction is conducted with purified water.

5. The method according to claim 1, characterized in that after each step of extracting spend insistence, and after the first stage maceration is carried out in the course of 170-190 min, and after the second stage within 110-130 minutes

6. The method according to claim 1, characterized in that the concentration of an aqueous extract of artichoke in vacuum is performed at a pressure vacuum 0,070-0,080 MPa and steam pressure 0,20-0,30 MPa.

7. The method according to claim 1, characterized in that the filler take microcrystalline cellulose.

8. The method according to claim 1, characterized in that as baking powder take Aerosil or silicon dioxide.

9. The method according to claim 1, characterized in that the moving substance take stearic acid or its salts.

10. The method according to claim 1, characterized in that the drug capsulebuy.



 

Same patents:

FIELD: medicine, biotechnology, healthy medical and veterinary preparations.

SUBSTANCE: claimed method includes exposing of aqueous isotonic solution of sugar-containing raw materials such as mixture of glucose and fructose in mass ratio of 1:1 with gamma-irradiation in absorbed dose of 25-40 kGy.

EFFECT: agent of standard composition with high antiviral and hepatoprotective action.

2 tbl, 2 ex

FIELD: biology, medicine.

SUBSTANCE: invention relates to cartiotrofin (CT-1) expression enhancing in process of liver regeneration which consists with maximal hepatocyte proliferation. Also invention relates to application of CT-1 and composition based on the same as stimulator of liver regeneration, as well as hepatoprotective effect of CT-1 in various models of acute liver lesions.

EFFECT: application of CT-1 to improve liver functions at various pathologies thereof.

10 cl, 14 ex, 13 dwg

FIELD: medicine, hepatology.

SUBSTANCE: at entering a hospital, one should detect patient's percentage content of lymphocytes in blood, and at its value being under 18% it is necessary to inject myelopid, every other day, 5 injections/course. Then comes conventional therapy of the above-mentioned disease. The innovation provides earlier prescription of immunocorrecting preparation as myelopid at acute period of the disease in question due to simple, objective criterion of individual necessity for myelopid prescription.

EFFECT: higher efficiency of therapy.

1 cl, 3 ex

FIELD: medicine, pediatrics.

SUBSTANCE: children should take sulfide baths and for inner intake - warm low-carbonate chloride-hydrocarbonade sodium-calcium water of low mineralization per 100-200 g depending upon patient's age, for 30 d. The innovation provides improved clinical and paraclinical values due to combined intake of sulfide baths, that stimulate nervous centers through vast cutaneous receptor fields, and drinking water of concrete ionic composition and mineralization degree that decreases activity of all enzymatic systems of gastro-intestinal tract, hepatic among them.

EFFECT: higher efficiency of therapy.

2 ex, 3 tbl

FIELD: medicine, abdominal surgery.

SUBSTANCE: one should drain biliary ducts and wash with 0.02%-sodium hypochlorite solution at the dosage of 150-200 ml, by drops, 3-4 times daily for 7-17 d. Moreover, also, during 7-17 d it is necessary to carry out infusion therapy through catheterized portal vein by applying conventional medicinal preparations and introduce 0.04%-sodium hypochlorite per 50-80 ml thrice daily. The innovation provides therapeutic efficiency at applying sodium hypochlorite in lower concentration against well known for sanitation of biliary tract that enables to decrease toxic effect of sodium hypochlorite during therapy conducted.

EFFECT: higher efficiency of therapy.

3 ex, 1 tbl

FIELD: pharmaceutical chemistry.

SUBSTANCE: invention deals with creating pharmaceutical compositions containing bile acids and methods of treatment using these compositions. For this purpose, invention provides transparent aqueous solution containing bile acid or its compounds, polysaccharide, and water. Amounts of the two formers are selected so that they remain in solution at all values within selected pH range. Administration of such a composition increases level of enterohepatic bile acid in body, including blood, and also increases absorption of bile acid. Addition of various drug to composition allows effective treatment of corresponding diseases.

EFFECT: improved transportation to body tissues of bile acids both as individual therapeutic agents and as drug forms.

47 cl, 11 dwg, 16 tbl, 18 ex

FIELD: medicine.

SUBSTANCE: method involves administering Corbiculin at a dose of 2 g three times a day when prediluted with 50 ml of water. The treatment course is 21 days long or longer until alanine aminotransferase and aspartate aminotransferase indices assume normal values.

EFFECT: enhanced effectiveness in normalizing hyperenzymemia and relieving clinic manifestations of cytolytic syndrome.

2 tbl

FIELD: medicine, hepatology.

SUBSTANCE: the present innovation deals with treating hepatitis and hepatic cirrhosis of different etiology. For this purpose, in case of viral hepatitis B and C at the stage of active viral replication one should inject sodium hypochlorite solution into central vein at concentration of 300-600 mg/l at the rate of 60 drops/min, per about 200-400 ml every other day, about 5-7 procedures/course; in case of viral hepatitis B and C beyond the stage of active viral replication one should inject sodium hypochlorite solution into peripheral vein at concentration of 300 mg/l at the rate of 30 drops/min per about 200-400 ml every other day, about 5-7 procedures/course; in case of hepatitis of non-viral etiology one should inject sodium hypochlorite solution into peripheral vein at concentration of about 200-300 mg/l at the rate of 30 drops/min, per about 200-400 ml daily, about 3-5 procedures/course; in case of hepatic cirrhosis it is necessary to inject sodium hypochlorite solution into peripheral vein at concentration of about 100-200 mg/l at the rate of 40 drops/min, per about 200-400 ml every other day, about 3-5 procedures/course. The innovation suggested provides increased efficiency of detoxication and anti-viral impact due to differentiated matching both the dosages and modes of preparation injection at different forms of hepatic lesions.

EFFECT: higher efficiency of therapy.

2 ex, 1 tbl

FIELD: medicine, gastroenterology, phytotherapy.

SUBSTANCE: invention relates to the development of agent used in treatment of stomach and duodenum ulcer disease with eradication of microorganism Helicobacter pylori occurring on the background of liver chronic diseases. Agent represents a species of medicinal plants and comprises common milfoil, fenestrate Saint-John's-wort, medicinal matricary, sweet flag rhizome with roots, common wormwood, sandy immortelle, black elder flowers, medicinal pot-marigold, peppermint, sage, dioecious nettle, umbellate centaury, medicinal sweet clove, medicinal dandelion roots, great burnet rhizomes, gum-tree leaves, tansy, maize stigmas, creeping thyme, cowberry leaves, flax seeds, tormentil rhizomes, linden flowers, common bear berry taken in the therapeutic dosage and in the definite mass ratio of components. The agent reduces the toxic effect of hepatobiliary system and prolongs the remission periods.

EFFECT: improved, enhanced and valuable medicinal properties of agent.

2 tbl, 4 ex

FIELD: medicine, pharmaceutical industry, phytotherapy.

SUBSTANCE: invention proposes an agent used in treatment of infectious diseases. Agent used in treatment of infectious diseases comprises chitosan of polyfraction composition with amine groups of molecular mass from monomeric link - glucosamine to ˜350 kDa and deacetylation degree from ˜68 to ≤ 95% and vegetable raw aqueous extract chosen from the following group: Cetraria thallus, sage official herb, cocklebur leaves, black poplar buds, common wormwood herb, red roots, burdock roots, French honey-suckle herb, bur-marigold tripartite herb, sandy common immortelle flowers, dropwort elm-leaved flowers, river avens (Geum) roots, licorice glabrous roots, swampy sweet flag roots, burnet official roots,, nettle dioecious herb, swampy cudweed herb, common milfoil herb, sweet clover official herb, gum-free leaves, dropwort, willow bark, birch leaves, bilberry leaves, willow-herb narrow-leaves herb, peppermint herb, fenestrate Saint-John's-wort herb, knot-grass herb, shepherd's purse herb, violet pensy tricolor herb, speedwell herb, pot-marigold flowers, common tansy flowers, matricary official flowers, chicory roots, dandelion official roots, tormentil roots, cinnamon wild rose fruits, viburnum leaves, pinnate kalanchoe and maize stigmas. Aqueous extract is prepared by extraction of vegetable raw with demineralized water under definite conditions. Agent promotes to effective treatment of infectious diseases, such as chlamydiosis, herpes, erysipelas, viral hepatitis A and B, gastroenteric tract diseases, acute enteric infectious, and to declining period of clinical symptoms of diseases said.

EFFECT: valuable medicinal properties of agent.

10 cl, 2 dwg, 12 ex

FIELD: pharmaceutical industry, in particular production of pharmaceutical agent having sedative action.

SUBSTANCE: claimed method includes breakage of pharmaceutical plant raw materials, preparing of dry extract therefrom by sequential extraction with hot water under certain conditions and separation of liquid extract from solvent cake, filtration and boiling down, second filtration and drying followed by obtained dry extract with filler, wherein as plant raw materials motherwort grass, hop cones, peppermint leaves, valerian foots and rootstocks, and liquoris root taken in specific ratio are used. Pharmaceutical agents may be in form of capsules, grains, or pellets.

EFFECT: drugs with enhanced pharmaceutical action and high therapeutic activity in decreased dose; simplified method of high yield; bioactive substances of increased stability.

5 cl, 4 ex

FIELD: pharmaceutical industry, in particular production of pharmaceutical agent having sedative action.

SUBSTANCE: claimed method includes breakage of pharmaceutical plant raw materials, preparing of dry extract therefrom by sequential extraction with hot water under certain conditions and separation of liquid extract from solvent cake, filtration and boiling down, second filtration and drying followed by obtained dry extract with filler, wherein as plant raw materials motherwort grass, hop cones, peppermint leaves, valerian foots and rootstocks, and liquoris root taken in specific ratio are used. Pharmaceutical agents may be in form of capsules, grains, or pellets.

EFFECT: drugs with enhanced pharmaceutical action and high therapeutic activity in decreased dose; simplified method of high yield; bioactive substances of increased stability.

5 cl, 4 ex

FIELD: pharmaceutical industry, in particular production of pharmaceutical agent having sedative action.

SUBSTANCE: claimed method includes breakage of pharmaceutical plant raw materials, preparing of dry extract therefrom by sequential extraction with hot water under certain conditions and separation of liquid extract from solvent cake, filtration and boiling down, second filtration and drying followed by obtained dry extract with filler, wherein as plant raw materials motherwort grass, hop cones, peppermint leaves, valerian foots and rootstocks, and liquoris root taken in specific ratio are used. Pharmaceutical agents may be in form of capsules, grains, or pellets.

EFFECT: drugs with enhanced pharmaceutical action and high therapeutic activity in decreased dose; simplified method of high yield; bioactive substances of increased stability.

5 cl, 4 ex

FIELD: pharmaceutical industry, in particular production of pharmaceutical agent having sedative action.

SUBSTANCE: claimed method includes breakage of pharmaceutical plant raw materials, preparing of dry extract therefrom by sequential extraction with hot water under certain conditions and separation of liquid extract from solvent cake, filtration and boiling down, second filtration and drying followed by obtained dry extract with filler, wherein as plant raw materials motherwort grass, hop cones, peppermint leaves, valerian foots and rootstocks, and liquoris root taken in specific ratio are used. Pharmaceutical agents may be in form of capsules, grains, or pellets.

EFFECT: drugs with enhanced pharmaceutical action and high therapeutic activity in decreased dose; simplified method of high yield; bioactive substances of increased stability.

5 cl, 4 ex

FIELD: pharmaceutical industry, in particular production of pharmaceutical agent having sedative action.

SUBSTANCE: claimed method includes breakage of pharmaceutical plant raw materials, preparing of dry extract therefrom by sequential extraction with hot water under certain conditions and separation of liquid extract from solvent cake, filtration and boiling down, second filtration and drying followed by obtained dry extract with filler, wherein as plant raw materials motherwort grass, hop cones, peppermint leaves, valerian foots and rootstocks, and liquoris root taken in specific ratio are used. Pharmaceutical agents may be in form of capsules, grains, or pellets.

EFFECT: drugs with enhanced pharmaceutical action and high therapeutic activity in decreased dose; simplified method of high yield; bioactive substances of increased stability.

5 cl, 4 ex

FIELD: medicine, mycology.

SUBSTANCE: invention proposes a medicinal agent against human candidosis that comprises an active component as inactivated cytoplasm fraction form the fungus strain Candida albicans and a solvent. An active component represents fungus cellular walls and cytoplasm after culturing the fungus strain Candida albicans № 253 for 28-36 h and shows the fungus cells concentration 1.85 x 108 - 2.5 x 108/1 cm3 of distilled water, immunogenicity index is 6-9%, the content of lipids is up to 0.2%, polysaccharide is 50-76%, peptides with molecular mass > 10 kDa is 80-87%. The agent comprises formalin as an inactivating agent and distilled water as a solvent in the following ratio of components, wt.-%: active component as cellular walls and cytoplasm from the fungus strain Candida albicans № 253 with the concentration of fungus spores 1.85 x 108 - 2.5 x 108/1 cm3 of distilled water, 2.0-2.7; formalin, 0.1-0.15, and water, the balance. Invention provides the effective and safety agent against human candidosis. Invention can be used in treatment of candidosis.

EFFECT: enhanced effectiveness and valuable medicinal properties of agent.

4 ex

FIELD: cosmetology, in particular cream production.

SUBSTANCE: claimed method includes preparation of fresh vegetable juice or mixture thereof, filtering, concentration at temperature up to 40°C followed by dissolution in structured water, obtained from plants, plant raw materials, slush, or in structured water, ionized, for example, with copper ions and optionally aseptic treatment of obtained product not causing denaturation, such as by membrane filtration method.

EFFECT: new matters for cosmetic products.

1 ex

FIELD: medicine, gynecology.

SUBSTANCE: invention relates to a method for treatment of fallopian-peritoneal form of infertility suffering with chronic endometritis. After carrying out reconstructive endoscopic operations in small pelvis cavity under control of histerscope tool a medicinal cocktail is administrated by intrauterine route by the following schedule: at the first and third days of treatment - prednisolon in the dose 30 mg, dioxydin in the dose 50 ml of 0.5% solution, metrogil in the dose 5.0 ml, lydaze in the dose 32 IU, antibiotic - "Makmimor" suppository before night by vaginal route and vagina syringe with matricary solution in the morning. At the second and fourth days of treatment prednisolon is administrated in the dose 30 mg by intrauterine route, "Instillagel" in the dose 6-11 mg into vagina posterior fornix in the dose one tampon with 20 ml of Vishnevsky's ointment. On the fifth, seventh and ninth days dioxydine is administrated in the dose 50 ml of 0.5% solution by intrauterine route and also 5.0 ml of metrogil, 0.5 ml of cycloferon, 32 IU of lydaze, antibiotic, and "Makmimor" suppositories are administrated by vaginal rout before night, and vagina syringe with matricary solution is carried out in the morning. On sixth, eighth and tenth days of treatment "Instillagel" is administrated in the dose 6 ml by intrauterine route, dimexide in the dose 5 ml and tampon with 20 ml of Vishnevsky's ointment is administrated into vagina posterior fornix before night. Since the 1-st day of treatment wobenzym is prescribed in the dose 5 tablets, 3 times per a day for 14 days and cyclic hormonal therapy for 3 months. Method provides the full-value elimination of pathogens from uterine cavity, normalization of vagina biocenosis and recovery of the menstrual function.

EFFECT: improved and enhanced method of treatment.

4 tbl, 1 ex

FIELD: medicine, biotechnology, healthy medical and veterinary preparations.

SUBSTANCE: claimed method includes exposing of aqueous isotonic solution of sugar-containing raw materials such as mixture of glucose and fructose in mass ratio of 1:1 with gamma-irradiation in absorbed dose of 25-40 kGy.

EFFECT: agent of standard composition with high antiviral and hepatoprotective action.

2 tbl, 2 ex

FIELD: medicine, phthisiology.

SUBSTANCE: method involves firstly the achievement of lymphotropicity of three chemopreparations by addition of 5% glucose and aloe to solutions of these chemopreparations. Then the conduction paravertebral anesthesia is carried out at the level and at side of administration of preparations. Then three chemopreparations are administrated separately in different intercostals sites, 1-3 times per a week, course of 4-12 injections by subcutaneous paravertebral route, parasternal route in I-X intercostals - in projection of regional lymphatic collectors. Method allows reducing the duration of intensive phase in tuberculosis treatment up to 1-3-6 months, to prevent the development of drug-resistant tuberculosis and adverse effects of chemopreparations and to relieve the residual changes of tuberculosis. Invention can be used in treatment of infiltrative, destructive and drug-resistant pulmonary tuberculosis.

EFFECT: improved and enhanced method of treatment.

2 ex

FIELD: pharmaceutical industry, in particular production of pharmaceutical agent having sedative action.

SUBSTANCE: claimed method includes breakage of pharmaceutical plant raw materials, preparing of dry extract therefrom by sequential extraction with hot water under certain conditions and separation of liquid extract from solvent cake, filtration and boiling down, second filtration and drying followed by obtained dry extract with filler, wherein as plant raw materials motherwort grass, hop cones, peppermint leaves, valerian foots and rootstocks, and liquoris root taken in specific ratio are used. Pharmaceutical agents may be in form of capsules, grains, or pellets.

EFFECT: drugs with enhanced pharmaceutical action and high therapeutic activity in decreased dose; simplified method of high yield; bioactive substances of increased stability.

5 cl, 4 ex

Up!