Method for production of sugar-based agents having antiviral and hepatoprotective action
FIELD: medicine, biotechnology, healthy medical and veterinary preparations.
SUBSTANCE: claimed method includes exposing of aqueous isotonic solution of sugar-containing raw materials such as mixture of glucose and fructose in mass ratio of 1:1 with gamma-irradiation in absorbed dose of 25-40 kGy.
EFFECT: agent of standard composition with high antiviral and hepatoprotective action.
2 tbl, 2 ex
The invention relates to medicine and biotechnology and can be used to produce the sugars treatment-and-prophylactic drugs for medical and veterinary purposes.
You know getting biostimulant by separating the antigen from bacterial cells or their metabolic products containing patterns belonging to the group of sugars (patent applications France No. 2399845, publ. 13.06.79, MKI A 61 K 39; EPO No. 0045718, publ. 02.10.82, MKI a 61 K 9/06).
These methods of ethnologica, as they require a high degree of purification of secreted active components and their subsequent binding with a pharmaceutical carrier. This reduces biological activity of the target product.
Also known is a method of obtaining biostimulating funds from sugar-containing raw materials by dissolving the honey in water to a hypertonic concentration (Joiris BTW, the Curative properties of honey and bee venom, M., "Midges", 1956, s-136; Kuzmin K.A. Treatment of bee honey and poison, publishing house of Saratov Univ-TA, 1983).
Target product, obtained in a known manner, has a low biological activity. In addition, it is non-standard, because of the complexity and produces different varieties of honey.
Closest to the claimed technical essence is a way of obtaining drugs, budushego antivirus, hepatoprotective and immunomodulatory activity (Patent RF №2155051, epubl. BI No. 24 - prototype)by fractionation of a solution floral honey crystallization in ice, incubation within 3-30 days at a temperature 4-30°s, respectively, followed by clarification by filtration or centrifugation, freezing and radiation treatment to the absorbed dose of 0.1-100 kGy.
There is a method allows to obtain a biologically active agent with a complex therapeutic properties, but having a non-standard composition due to the use as a raw material of the non-standard multi-component substances, namely - flower honey. Furthermore, the method is sequential and time-consuming.
The objective of the proposed technical solution is the standardization of the target product, while maintaining its biological activity and simplification of the method of its production.
The problem is solved by ionizing radiation absorbed dose is 10 to 40 kGy) on aqueous isotonic solution of sugar containing raw material, which is used as glucose and/or fructose.
The inventive method allows to obtain the substance of the standard composition having a high protivovirusnoi and hepatoprotective activity of glucose and/or fructose. Compared to what procedure prototype, the proposed method is simpler and more accessible.
We have found that the radiolysis products of glucose and fructose or mixtures thereof have protivovirusnoi and hepatoprotective activity in terms of the absorbed dose of gamma radiation 10 to 40 kGy, and found that when used in the method of obtaining compounds having these kinds of biological activity of a mixture of two sugars: glucose and fructose, there is a synergistic effect of increasing this activity.
In the study of the prior art applicants were found similar way, with the above-mentioned differences from the prototype to achieve the same technical result, which is obtained in the present method. This allows to make a conclusion about conformity of the present invention, the criterion of "novelty" and "inventive step".
The inventive method also meets the criterion of "industrial applicability", because it offers the use of known materials and technical means.
The method is illustrated by the following examples.
EXAMPLE 1. Distilled water to prepare isotonic solutions of glucose, fructose, and mixtures thereof. Sample ingredients are taken from the calculation of 0.29 gram-molecules per 1 liter of solution (I. Belova, Karczewski CENTURIES, Rudakova N.A. Sokolova, L. F. manufacturing Technology sterile RA the solutions in terms of pharmacies. M, "Medicine", 1982, p.51). So, based on the molecular weight of anhydrous glucose (fructose) 180,18, the amount of glucose (fructose) in 1 l of isotonic is: 0,h,18=52,25, Therefore, isotonic concentration of glucose, fructose or mixtures thereof) equal to 5.2 wt.%. Here and below we take equal sample sugars in the mixture. Similarly prepared control solutions, including additional sugar xylose. Another control is isotonic honey (prototype).
The prepared solutions are subjected to γ-irradiation dose of 10 kGy and 25 kGy. While the honey solution and the solutions of the mixture of sugars is divided into two parts, one of which left unirradiated (control). The biological activity of drugs is examined for white mice (10-14 individuals in the group). Prepared solutions administered intranasally 50 ál for 10 days, then spend a single seed mice intraperitoneally with 0.2 ml of 25% oil solution of the CCI4. The effect of the drugs is estimated by the survival of mice for 4 days. The results are shown in table 1.
The table shows that:
1) sugar xylose pronounced hepatoprotective effect does not possess;
2) of the tested sugars most potent hepatoprotective effect has fructose (death of mice 20%);
3) effect of fructose increases in the presence of glucose (with adequate isotonic concentration is the mixture of ingredients of the death of the mice just 7.7%);
4) γ-irradiation leads to increased hepatoprotective properties of the obtained products in 2-3 times;
5) irradiated mixed solution of glucose and fructose has a more pronounced hepatoprotective properties than the prototype.
EXAMPLE 2. Subjects and control preparations prepared as in example 1 at the absorbed dose of gamma radiation 40 kGy. They have an antiviral effect on the model experiencing fragments chorioallantois shell (FAO) chick embryos. For this influenza virus A/Hong Kong/1/68 (H3N2) infectious activity of 100 EID50/0.2 ml contribute to the wells planchettes with FAO in culture medium 199 and incubated 1 h at 37°for adsorption is carried virus. Later in the wells make subjects and control preparations in 20-fold dilution (10 holes on the drug) and incubated at 37°C for 3 days, after which determine residual infectious activity (IA) virus titration on chicken embryos. The results are shown in table 2.
As can be seen from table 2, the irradiated solutions of glucose and fructose have antiviral action, the most effective use of their irradiated mixture, which is somewhat superior to the prototype (the drug of honey solution).
As explained above examples, the use of the proposed method in comparison with the prototype allows jet to preserve the biological activity of the target product at a high level, and, in the case of using a mixture of glucose and fructose, even more to increase it. The inventive method provides for the standard description of the target product and is more simple in comparison with the method of the prototype.
Hepatoprotective effects of drugs after priming mice CCI4
|Ingredients preparation*||Absorbed dose, kGy||The percentage of death:|
|glucose||fructose||xylose||honey||1 day||4 days|
|(control: mice do not protect)||100,0||-|
|* Presence(+)or absence(-).|
Antiviral activity of drugs on the model FHA
a dose of 40 kGy
|Residual IA, lg EID50||Index reduction IA, lg EID50|
|(control: infected cells not treated with a protective tool)||5,5±0,03||-|
|* Presence(+)or absence(-).|
The method of obtaining funds on the basis of sugars with antiviral and hepatoprotective activity by exposure to gamma-irradiation at an absorbed dose of 25-40 kGy water isotonic solution of sugar containing raw material, characterized in that as the sugar-containing raw materials, use glucose and fructose in a ratio of 1:1 by weight.
FIELD: medicine, neurology, virology.
SUBSTANCE: invention relates to treatment of neurological diseases caused by herpes virus, such as Bell's paralysis, Hunt's disease, herpetic encephalitis accompanying with damage of cerebral nerves. Invention involves using 1,4-dihydropyridine blockers of calcium channels, such as felodipine, nifedipine, nimodipine, nisodipine being taken preferably in combination with herpes virus antagonist. Invention provides repairing damaged cerebral nerves by topical expanding arteriols and recovery of local microcirculation based on specific competitive interaction of definite groups of calcium blockers of 1,4-dihydropyridine type with vasoconstrictor endothelin.
EFFECT: enhanced effectiveness of treatment.
47 cl, 2 dwg, 1 ex
FIELD: medicine, pharmacy.
SUBSTANCE: invention relates to a cycloferon-containing medicinal agent used in prophylaxis and treatment of influenza and acute respiratory diseases, herpetic infection, chronic viral hepatitis B and C and prophylaxis of oncological diseases. Agent comprises a physiological solution in the following ratio of components: 10-29 - 10-2 mg of cycloferon in 1 ml of physiological solution. Also, invention relates to a method for preparing this medicinal agent. Proposed medicinal agent enhances antiviral and antitumor activity of natural killers by 1.8-fold, not less.
EFFECT: improved preparing and using method, valuable medicinal properties of agent.
7 cl, 1 dwg, 19 ex
FIELD: veterinary science.
SUBSTANCE: it is necessary to inject birch bark extract for the animal at the dosage of 20 or 40 mg/kg body weight. The present innovation enables to increase leukocytic immunocompetence that, in its turn, prevents viral proliferation and disease relapses.
EFFECT: higher efficiency of therapy.
2 cl, 1 dwg, 4 ex
SUBSTANCE: the suggested strain refers to DNA-containing Adenoviridae type being of cubic type of symmetry, has no supercapsid membrane, virions sizes - 70-80 nm, shape - icosahedral. Moreover, it is resistant to the temperature of 56°C for 30 min, not less, and to the action of both ether and chloroform. It is inactivated with formalin, theotropin, dimer ethylenimine. The strain is cultivated in hepatic cell culture of hen's embryos, about 72-96 h after infection it causes characteristic foci of granulation (cells' rounding), the formation of separate rounded cells and foci of cell degeneration. Viral titer is within 105.5 TCD50/ml. The strain enables to reproduce different forms of the disease mentioned for experimental investigations, it is highly useful for manufacturing certain diagnostic kits and vaccines, moreover, being of high antigenic and immunogenic activity.
EFFECT: higher efficiency.
FIELD: medicine, virology, pharmacology.
SUBSTANCE: invention relates to an antiviral substance based on hemolymph of insects of subclass Pterigota. This substance is prepared by activation of immune system of insects, collection of hemolymph and the following treatment of prepared hemolymph by centrifugation, chromatography of supernatant, elution of hydrophobic components sobbed on column. Prepared eluate comprises a mixture of hydrophobic components possessing with an antiviral activity. Invention provides the direct antiviral effect and enhances resistance of body cells to viral infection by virus disruption or blocking its replication, or by other method.
EFFECT: improved preparing method, valuable antiviral properties of substance.
41 cl, 1 tbl, 1 dwg, 1 ex
FIELD: immunology, molecular biology.
SUBSTANCE: invention relates to a method for presentation of viral antigens of the host immune system based on proteasome-mediated technology designated for development of methods of biological protection. Method involves using recombinant plasmids pCI-neo-ODS-nsP1, pCI-neo-ODS-E2-L and pCI-neo-ODS-E2-M. Recombinant plasmids pCI-neo-ODS-nsP1, pCI-neo-ODS-E2-L and pCI-neo-ODS-E2-M are prepared by the successive cloning gene-equivalents of nonstructural protein nsP1, L and M fragments of structural protein E2 of virus VEL, respectively, as components of gene encoding enzyme ornitine decarboxylase being firstly into prokaryotic plasmid vector pET-23b and then into eucaryotic plasmid vector pCI-neo. The advantage of invention involves the development of polynucleotide vaccines inducing the cell-mediated immune response. The proposed invention represents the firstly developed in Russian Federation method of DNA vaccination based on using proteasome-mediated technology as result of molecular-biological and immunological investigations.
EFFECT: improved method for presentation of antigens.
4 tbl, 1 ex
FIELD: organic chemistry, biochemistry, medicine.
SUBSTANCE: invention relates to a new biologically active compound of 4-oxoquinoline that is useful as an anti-HIV agent and to its pharmaceutically acceptable salt. Invention describes an anti-HIV agent comprising compound of 4-oxoquinoline represented by the following formula [I] or its pharmaceutically acceptable salt as an active component wherein ring Cy represents phenyl group, naphthyl group or pyridyl group and each this group is substituted optionally with 1-5 substituted chosen from the following group A wherein A represents the group consisting of cyano-group, phenyl group, nitro-group, halogen atom, (C1-C4)-alkyl group, halogen-(C1-C4)-alkyl group, halogen-(C1-C4)-alkoxy-group, -ORa1, -SRa1, -NRa1Ra2, -CONRa1Ra2, -SO2NRa1Ra2, -NRa1CORa3, -SO2Ra3, -NRa1SO2Ra3 and -COORa1 wherein Ra1 and Ra2 are similar or different and each represents hydrogen atom, (C1-C4)-alkyl group or benzyl group, and Ra3 represents (C1-C4)-alkyl group; R1 represent a substitute chosen from the following group B, or (C1-C10)-alkyl group optionally substituted with 1-3 substitutes chosen from halogen atom and the following group B wherein the group B represents the group consisting of phenyl group optionally substituted with phenyl group or 1-5 halogen atoms; (C3-C6)-cycloalkyl group, imidazolyl group, benzothiophenyl group, thiazolyl group optionally substituted with 1-3 (C1-C6)-alkyl groups, morpholinyl group, pyridyl group, -ORa4, -SRa4, -NRa4Ra5, -CONRa4Ra5, -SO2NRa4Ra5, -CORa6, -NRa4CORa6, -SO2Ra6, -NRa4SO2Ra6, -COORa4 and -NRa5COORa6 wherein Ra4 and Ra5 are similar or different and each represents hydrogen atom, (C1-C4)-alkyl group or phenyl group; Ra6 represents (C1-C4)-alkyl group; R2 represents hydrogen atom or (C1-C4)-alkyl group; R31 represents hydrogen atom, cyano-group, hydroxy-group, halogen atom or (C1-C4)-alkoxy-group; X represents -C-R32, and Y represents -C-R33 or nitrogen atom wherein R32 and R33 are similar or different and each represents hydrogen atom, cyano-group, halogen atom, pyrrolidinyl group, (C1-C10)-alkyl group optionally substituted with 1-3 halogen atoms, -ORa7, -SRa7, -NRa7Ra8, -NRa7CORa9, -COORa10 or -N=CH-NRa10Ra11 wherein Ra7 and Ra8 are similar or different and each represents hydrogen atom, phenyl group or (C1-C10)-alkyl group optionally substituted with (C3-C6)-cycloalkyl group or hydroxy-group; Ra9 represents (C1-C4)-alkyl group and Ra10 and Ra11 are similar or different and each represents hydrogen atom or (C1-C4)-alkyl group. Also, invention describes compound of the formula (III) given in the invention description, integrase inhibitor, antiviral agent, ant-HIV composition, anti-HIV agent, using compound of 4-oxoqionoline, method for inhibition of integrase activity, method for prophylaxis or treatment of viral infectious disease, pharmaceutical composition used for inhibition of integrase activity, antiviral composition and commercial package (variants). Invention provides the development of a pharmaceutical agent possessing inhibitory effect on activity of integrase.
EFFECT: valuable medicinal properties of compound, agent and composition.
40 cl, 7 tbl, 250 ex
SUBSTANCE: method involves applying antiherpetic therapy using Phamcyclovir. Then, Trental is sequentially introduced during 5 days with 2-5 ml of cerebrolysin being concurrently intratissularly introduced behind mastoid process tip. Mexidol is administered for 10 days. The treatment is finished by introducing Polyoxydonium.
EFFECT: combined microcirculation improvement; high neurotrophic and antioxidant activity; secondary immune deficiency adjustment; eliminated labyrinth hydrops.
FIELD: immunology, biotechnology.
SUBSTANCE: invention describes antibody and its fragments neutralizing rabies virus and a method for treatment of patient subjected for effect of rabies virus by using indicated antibody and its fragment. Invention discloses variants of isolated nucleic acids encoding polypeptides carrying light and heavy chain of antibody, respectively. Also, invention describes expressing vector carrying at least one of indicated nucleic acids. Using this invention enhances span-life of patients after effect with rabies virus on them and can be used in corresponding prophylactic therapy of such patients.
EFFECT: valuable medicinal properties of antibody and nucleic acid.
14 cl, 1 dwg, 1 tbl, 2 ex
FIELD: medicine, organic chemistry, pharmacy.
SUBSTANCE: invention relates to an antibacterial preparation based on α-aminosulfonic acid amide, i. e. N'-methyl-1-phenyl-1-N,N-diethylaminomethanesulfonamide that can be used in veterinary science. Invention relates to expanding assortment of agents used against pathogenic pathogen with a preparation relating to class of α-aminosulfonic acid amides eliciting antibacterial activity being without cumulative properties and pathological changes in animal organs and tissues.
EFFECT: valuable veterinary properties of preparation.
1 tbl, 1 ex
FIELD: biology, medicine.
SUBSTANCE: invention relates to cartiotrofin (CT-1) expression enhancing in process of liver regeneration which consists with maximal hepatocyte proliferation. Also invention relates to application of CT-1 and composition based on the same as stimulator of liver regeneration, as well as hepatoprotective effect of CT-1 in various models of acute liver lesions.
EFFECT: application of CT-1 to improve liver functions at various pathologies thereof.
10 cl, 14 ex, 13 dwg
FIELD: medicine, hepatology.
SUBSTANCE: at entering a hospital, one should detect patient's percentage content of lymphocytes in blood, and at its value being under 18% it is necessary to inject myelopid, every other day, 5 injections/course. Then comes conventional therapy of the above-mentioned disease. The innovation provides earlier prescription of immunocorrecting preparation as myelopid at acute period of the disease in question due to simple, objective criterion of individual necessity for myelopid prescription.
EFFECT: higher efficiency of therapy.
1 cl, 3 ex
FIELD: medicine, pediatrics.
SUBSTANCE: children should take sulfide baths and for inner intake - warm low-carbonate chloride-hydrocarbonade sodium-calcium water of low mineralization per 100-200 g depending upon patient's age, for 30 d. The innovation provides improved clinical and paraclinical values due to combined intake of sulfide baths, that stimulate nervous centers through vast cutaneous receptor fields, and drinking water of concrete ionic composition and mineralization degree that decreases activity of all enzymatic systems of gastro-intestinal tract, hepatic among them.
EFFECT: higher efficiency of therapy.
2 ex, 3 tbl
FIELD: medicine, abdominal surgery.
SUBSTANCE: one should drain biliary ducts and wash with 0.02%-sodium hypochlorite solution at the dosage of 150-200 ml, by drops, 3-4 times daily for 7-17 d. Moreover, also, during 7-17 d it is necessary to carry out infusion therapy through catheterized portal vein by applying conventional medicinal preparations and introduce 0.04%-sodium hypochlorite per 50-80 ml thrice daily. The innovation provides therapeutic efficiency at applying sodium hypochlorite in lower concentration against well known for sanitation of biliary tract that enables to decrease toxic effect of sodium hypochlorite during therapy conducted.
EFFECT: higher efficiency of therapy.
3 ex, 1 tbl
FIELD: pharmaceutical chemistry.
SUBSTANCE: invention deals with creating pharmaceutical compositions containing bile acids and methods of treatment using these compositions. For this purpose, invention provides transparent aqueous solution containing bile acid or its compounds, polysaccharide, and water. Amounts of the two formers are selected so that they remain in solution at all values within selected pH range. Administration of such a composition increases level of enterohepatic bile acid in body, including blood, and also increases absorption of bile acid. Addition of various drug to composition allows effective treatment of corresponding diseases.
EFFECT: improved transportation to body tissues of bile acids both as individual therapeutic agents and as drug forms.
47 cl, 11 dwg, 16 tbl, 18 ex
SUBSTANCE: method involves administering Corbiculin at a dose of 2 g three times a day when prediluted with 50 ml of water. The treatment course is 21 days long or longer until alanine aminotransferase and aspartate aminotransferase indices assume normal values.
EFFECT: enhanced effectiveness in normalizing hyperenzymemia and relieving clinic manifestations of cytolytic syndrome.
FIELD: medicine, hepatology.
SUBSTANCE: the present innovation deals with treating hepatitis and hepatic cirrhosis of different etiology. For this purpose, in case of viral hepatitis B and C at the stage of active viral replication one should inject sodium hypochlorite solution into central vein at concentration of 300-600 mg/l at the rate of 60 drops/min, per about 200-400 ml every other day, about 5-7 procedures/course; in case of viral hepatitis B and C beyond the stage of active viral replication one should inject sodium hypochlorite solution into peripheral vein at concentration of 300 mg/l at the rate of 30 drops/min per about 200-400 ml every other day, about 5-7 procedures/course; in case of hepatitis of non-viral etiology one should inject sodium hypochlorite solution into peripheral vein at concentration of about 200-300 mg/l at the rate of 30 drops/min, per about 200-400 ml daily, about 3-5 procedures/course; in case of hepatic cirrhosis it is necessary to inject sodium hypochlorite solution into peripheral vein at concentration of about 100-200 mg/l at the rate of 40 drops/min, per about 200-400 ml every other day, about 3-5 procedures/course. The innovation suggested provides increased efficiency of detoxication and anti-viral impact due to differentiated matching both the dosages and modes of preparation injection at different forms of hepatic lesions.
EFFECT: higher efficiency of therapy.
2 ex, 1 tbl
FIELD: medicine, gastroenterology, phytotherapy.
SUBSTANCE: invention relates to the development of agent used in treatment of stomach and duodenum ulcer disease with eradication of microorganism Helicobacter pylori occurring on the background of liver chronic diseases. Agent represents a species of medicinal plants and comprises common milfoil, fenestrate Saint-John's-wort, medicinal matricary, sweet flag rhizome with roots, common wormwood, sandy immortelle, black elder flowers, medicinal pot-marigold, peppermint, sage, dioecious nettle, umbellate centaury, medicinal sweet clove, medicinal dandelion roots, great burnet rhizomes, gum-tree leaves, tansy, maize stigmas, creeping thyme, cowberry leaves, flax seeds, tormentil rhizomes, linden flowers, common bear berry taken in the therapeutic dosage and in the definite mass ratio of components. The agent reduces the toxic effect of hepatobiliary system and prolongs the remission periods.
EFFECT: improved, enhanced and valuable medicinal properties of agent.
2 tbl, 4 ex
FIELD: medicine, pharmaceutical industry, phytotherapy.
SUBSTANCE: invention proposes an agent used in treatment of infectious diseases. Agent used in treatment of infectious diseases comprises chitosan of polyfraction composition with amine groups of molecular mass from monomeric link - glucosamine to ˜350 kDa and deacetylation degree from ˜68 to ≤ 95% and vegetable raw aqueous extract chosen from the following group: Cetraria thallus, sage official herb, cocklebur leaves, black poplar buds, common wormwood herb, red roots, burdock roots, French honey-suckle herb, bur-marigold tripartite herb, sandy common immortelle flowers, dropwort elm-leaved flowers, river avens (Geum) roots, licorice glabrous roots, swampy sweet flag roots, burnet official roots,, nettle dioecious herb, swampy cudweed herb, common milfoil herb, sweet clover official herb, gum-free leaves, dropwort, willow bark, birch leaves, bilberry leaves, willow-herb narrow-leaves herb, peppermint herb, fenestrate Saint-John's-wort herb, knot-grass herb, shepherd's purse herb, violet pensy tricolor herb, speedwell herb, pot-marigold flowers, common tansy flowers, matricary official flowers, chicory roots, dandelion official roots, tormentil roots, cinnamon wild rose fruits, viburnum leaves, pinnate kalanchoe and maize stigmas. Aqueous extract is prepared by extraction of vegetable raw with demineralized water under definite conditions. Agent promotes to effective treatment of infectious diseases, such as chlamydiosis, herpes, erysipelas, viral hepatitis A and B, gastroenteric tract diseases, acute enteric infectious, and to declining period of clinical symptoms of diseases said.
EFFECT: valuable medicinal properties of agent.
10 cl, 2 dwg, 12 ex
FIELD: medicine, hepatology, chemical-pharmaceutical industry, biotechnology.
SUBSTANCE: invention relates to a biotransplant used in treatment of chronic hepatitis and liver cirrhosis and comprising mesenchymal stem cells obtained from fetal or donor material. The parent tissue is subjected for disaggregation followed by culturing as fixed colonies in the growth medium containing fetal calve serum and glutamine and passage at low density value with change of medium composition and cultivation is carried out without accumulation cells with mature stroma in culture. Also, invention relates to a method for treatment of chronic hepatitis and liver cirrhosis that involves administration of indicated biotransplants by using venous catheter or by puncture in the portal system veins, or by using arterial catheter in splenic artery, or by puncture in spleen parenchyma, or by intraperitoneal route, or by puncture in gastrocolic omentum. Invention provides enhancing effectiveness in complex effect on damaged liver.
EFFECT: improved preparing method, improved treatment method.
FIELD: medicine, phthisiology.
SUBSTANCE: method involves firstly the achievement of lymphotropicity of three chemopreparations by addition of 5% glucose and aloe to solutions of these chemopreparations. Then the conduction paravertebral anesthesia is carried out at the level and at side of administration of preparations. Then three chemopreparations are administrated separately in different intercostals sites, 1-3 times per a week, course of 4-12 injections by subcutaneous paravertebral route, parasternal route in I-X intercostals - in projection of regional lymphatic collectors. Method allows reducing the duration of intensive phase in tuberculosis treatment up to 1-3-6 months, to prevent the development of drug-resistant tuberculosis and adverse effects of chemopreparations and to relieve the residual changes of tuberculosis. Invention can be used in treatment of infiltrative, destructive and drug-resistant pulmonary tuberculosis.
EFFECT: improved and enhanced method of treatment.