Agent for early pathogenetic therapy of acute radiation disease

FIELD: medicine, medicinal radiobiology, pharmacy.

SUBSTANCE: invention proposes a multicomponent antioxidant complex comprising the following quenching agents of free radicals - ascorbic acid, alpha-tocopherol acetate, retinal acetate, unithiol and sodium selenite as a blocking agent of initiation of free-radical processes. By effect on viability of irradiated animals the invention exceeds the agent-prototype by 2-6 times. In distinction from the prototype, the claimed agent reduces the expression of maximal leukopenia by above two-fold, decreases the concentration of malonic dialdehyde by 1.5-2.0-fold, normalizes the intensity of free-radical oxidation in blood plasma and liver tissue and results to increasing activity of antioxidant systems. Invention can be used in treatment of acute radiation disease.

EFFECT: enhanced and valuable medicinal properties of agent.

6 tbl, 2 ex

 

The invention relates to medicine, namely to medical radiobiology, and can be used in the treatment of acute radiation sickness.

Acute radiation syndrome (ARS) is palindromic pathological process occurring after the overall impact on the human body to ionizing radiation (AI) in a total dose of about 1 Gy and above. Pathogenetic mechanisms of the development of the ARS associated with damaging effect of AI on rapidly proliferating tissues, particularly in the hematopoietic and immune systems. Caused by irradiation inhibition of myeloid and platelet sprouts of blood leads to the development pancytopenia syndrome, characterized by a sharp decline in the total number and functional activity of immunocompetent cells and platelets in the peripheral blood. As a consequence there are infectious complications and hemorrhagic manifestations that define the nature of the flow and outcome of the disease [1].

Current principles of treatment of patients with ARS provide aseptic mode, antibacterial, antiviral and antifungal drugs, the provision of opioid substitution therapy (plasma, leukocyte and platelet mass, transplantation of allogeneic bone marrow stem cells lane is fericelli blood), sessions of plasmapheresis, hemosorption and other Organization and conduct of these therapeutic and preventive measures is possible only in conditions of a specialized hospital, equipped with sophisticated and expensive equipment and has highly qualified medical personnel [2].

The results of several studies aimed at identifying the pathogenesis of ARS, suggest that one of the "trigger" mechanisms of development of radiation injury of the body is oxidative stress is a disturbance of homeostasis caused by over production of prooxidants and / or failure of the antioxidant defense systems [3, 4].

Currently, there is compelling evidence that exposure causes a significant increase in the content of tissues (including blood) of water - and fat-soluble prooxidants, primarily reactive oxygen metabolites (AKM), products of lipid peroxidation (LPO) and nitric oxide [3-6]. These substances are highly reactogenna and uncontrolled increase of their content in the body causes cytotoxic, mutagenic and genotoxic effects. In particular, such AKM as superoxide anion radical, singlet oxygen, hydroxyl radical, hydrogen peroxide enhance radiation damage of the structure of DNA (deletion reason, the destruction of hydrogen and FOS is kefirnyh links, the collapse of desoxyribose, education delocalising links in the DNA strands, the breaking of these links DNA-protein, DNA-membrane etc), increase the number of single and double breaks in the DNA chain[3, 7, 8].

The products of oxidative degradation of lipids, especially unsaturated fatty acids - arachidonic, linolenic and other (gidroperekisi, epoxides, aldehydes, ketones) cause of renal function and structure of biological membranes, which is reflected, in particular, on the activity of membrane-bound enzymes and function of DNA-membrane complex [3, 9]. Especially pronounced Geno - and cytotoxic activity have such products FLOOR as malonic dialdehyde, acrolein, CROTONALDEHYDE dialdehyde, 2,3-epoxy-4-hydroxynonenal, damaging the structure of DNA, causing chromosomal aberrations, delay cell division and cell death [3]. Radiomimetic properties peroxidation products was the basis for their selection in the group of primary lipid radiotoxins, which plays a significant role in the further development of radiation damage cells [5].

The primary radiotoxins also include oxidation products of phenols (quinones and semifinale) and DNA bases - 8-exoticises, exoproduct cytosine and uracil [3].

In recent years shows that high ability to activate the chain of free radical reactions have the nitrogen oxides - produce nitrogen monoxide and peroxynitrite [3, 10, 11]. Irradiation activates NO-synthetase, and this process is initiated AKM [3].

The most pronounced radiomimetic properties of the peroxynitrite formed by the reaction of interaction of nitrogen monoxide with superoxide anion-radical. Found that peroxynitrite and its protonated form can cause breaks chains and oxidation of DNA bases, oxidation of lipids of biological membranes, damage to the structure of some proteins [11, 12].

These data strongly suggest that oxidative stress is one of the main mechanisms for the strengthening of primary radiation damage in critical structures of the target cells, which are DNA and biological membranes.

It is important to note that in the intact cell oxidative processes are under strict and extremely diverse control mechanisms of antioxidant protection, providing course of free radical reactions at the hospital level, necessary for normal functioning of the cell.

Among the most active non-enzymatic antioxidants, which are able to inhibit the free-radical oxidation processes include [3, 11-15]:

- thiol compounds (glutathione, cysteine, taurine);

- vitamins (ascorbic acid, Toko is Erol, beta-carotene);

biogenic amines (serotonin, histamine, catecholamines);

- oligopeptides (carnitine, carnosine, anserine, endorphins, enkephalins);

- polyphenols, phospholipids, ubiquinone.

In addition, in the process of evolution the aerobes for protection against reactive oxygen radicals developed a specialized system of enzymatic antioxidants: superoxide dismutase, catalase, glutathione-dependent peroxidase and transferase, glutathione reductase, ceruloplasmin [15].

Irradiation, as well as many other extreme factors lead to changes in the antioxidant system in the dynamics of this process is distinguished by the stage of mobilization, normalization and depletion [3]. If the resources of this protective system is exhausted, there is an imbalance between antioxidant and oxidative processes, which can cause irreversible damage to the genetic apparatus and cell death [3, 15].

It is shown that irradiation causes a significant disturbance as enzymatic and nonenzymatic antioxidant links protection. Reduced activity of catalase, superoxide dismutase, the content of beta-carotene, tocopherol and ubiquinone in mitochondria of hepatocytes [16, 17].

The detected decrease in the concentration of tissue thiols, in particular, reduced glutathione after radiation the impact in high doses. Particularly significant changes in the glutathione system (decrease in the content of reduced glutathione, reduced activity of glutathione reductase) were detected after irradiation in bone marrow [18]. The victims in the accident at the Chernobyl nuclear power plant in the latent ARS period (4-7 days after exposure) observed a decrease in the content of ceruloplasmin and the activities of superoxide dismutase on the background of high levels of malondialdehyde and hydroperoxides in membranes of erythrocytes [19].

The accumulated evidence of the activation of free radical oxidation and reduction of the level of endogenous antioxidants in the early stages after irradiation, was pathogenetic rationale for the use of antioxidants as a means of increasing the body's resistance to the effects of ionizing radiation. Most commonly this purpose, the use of vitamins C, E, bioflavonoids, thiamine and other b vitamins, carotenoids [20-26]. Known examples of the successful use of antioxidant complex "Vetoron (consisting of vitamins a, E, C), and multivitamin preparations "Alicetrowel", "Tetravalent", etc. to increase nonspecific resistance in liquidators of consequences of the accident at the Chernobyl nuclear power plant [21, 27]. Similar effects have been detected in the chronic experiment on dogs with imitation possible radiac the Onna environment of space flight [28]. However, the results of experimental studies with animals that were irradiated in lethal doses showed that the effectiveness of vitamin preparations as radioprotection means small and occurs only in conditions of prophylactic use. So, in [16, 29] studied the radioprotective effect of the drug, "AK", consisting of vitamins a, C and E. it is Shown that the introduction of complex mice 1 day before irradiation at doses causing the death of 70% of the animals increases their 30-day survival rate to 60%. However, the data obtained can serve as a proof of the existence of a complex radioprotective properties at higher (close to the minimum absolutely lethal) doses of radiation exposure and medical conditions (i.e. after irradiation) application of the formulation. This recipe is considered as a prototype.

In addition to vitamins, as radioprotective funds have investigated the antioxidant thiol endogenous compounds (glutathione) and exogenous (unithiol) origin, but their radioprotective effect was quite moderately and is shown only for prophylactic use [30-32].

Known data also study the influence of organic selenium compounds (through inclusion in a standard animal diet preparation "Selenium") on resistance to radiation purebred cap) is [33], however, information about the effectiveness of selenium in terms of its therapeutic applications are missing.

Given the above, the aim of the invention was to find a means of early pathogenetic antioxidant treatment of radiation injuries, effective in terms of impact on the body of ionizing radiation in doses close to absolutely deadly.

This goal is achieved by creating a multi-component antioxidant complex, including extinguishers free radicals - ascorbic acid, alpha tocopherol acetate, retinol acetate, unithiol and blocker initiation of free radical processes - sodium Selenite.

The possibility of achieving the goal of the invention is proved by the following examples.

Example 1. The experiments were performed on 105 nonlinear white mice - males weighing 18-22, Animals were subjected to a common single gamma irradiation on the installation IGUR-1 at a dose of 7.5 Gy at the dose rate of 1.25 G/min

Animals of the first group served as control exposure.

Animals of the second group after 0.5, 6, 24 and 48 h after irradiation was administered tool-prototype (drug, "AK"), which is a triple antioxidant complex of vitamins a (7 mg/kg intragastrically), E (20 mg/kg intragastrically), With (20 mg/kg intraperitoneally).

Animals of the third group at the same time after irradiation type and the claimed means, representing pathcomponents antioxidant complex of vitamins a (7 mg/kg intragastrically), E (20 mg/kg intragastrically), With (20 mg/kg intraperitoneally), unithiol (50 mg/kg intraperitoneally) and sodium Selenite (0.1 mg/kg intragastrically).

The criterion for assessing the damaging effects of radiation and therapeutic actions of drugs was 30-day survival of irradiated animals.

The results are shown in table 1, which shows that in mice irradiated in absolutely minimal lethal dose, the insertion of the prototype contributed to the survival of 20% of the individuals. When using the proposed drug in contrast to the means of the prototype is more pronounced radioprotective action. With the introduction of pathcomponents complex survival rate of mice was 45%, which is 2 times higher than when using the prototype.

Table 1

The influence of the proposed means of the prototype on the survival of mice irradiated at a dose of 7.7 G
Used toolThe number of animals
onlyof them survived
abs.%
Irradiation (control)45 00±2
Tool-prototype30620±71
The inventive tool301344±91,2
1- p<0.05 compared with the irradiated control;

2- p<0.05 compared with vehicle-prototype.

Thus, the impact on 30-day survival of mice irradiated in an absolutely lethal dose of the inventive tool 2 times greater than the tool prototype.

Example 2.

The experiments were performed on 104 nonlinear white rats-males weighing 180-220, Animals were subjected to a common single gamma irradiation on the installation IGUR-1 at a dose of 7.5 Gy at a dose rate of 1.25 G/min

Animals of the first group served as control exposure.

Rats of the second and third groups after 0.5, 6, 24 and 48 h after irradiation was administered, respectively, the tool is a prototype and the proposed remedy in doses described in example 1.

The criterion for assessing the damaging effects of radiation and therapeutic actions of drugs served 30-day survival of irradiated animals, the dynamics of peripheral blood counts (number of cells), the intensity of free radical oxidation (CPO) and activity of antioxidant system (AOS) in plasma and liver tissue in the first 2 days the donkey exposure.

The collection of material for research status of lipid peroxidation and antioxidant system was performed after 7, 25 and 49 hours from the time of radiation exposure after decapitate rats. Blood stabilized with 4% sodium citrate in a ratio of stabilizer: blood - 1:9, centrifuged for 20 min at a speed of 3000 Rev/min Then the plasma was collected by pipette, poured into pre-labelled plastic tubes for microprobe and stored in liquid nitrogen prior to the study. The liver of animals after laundering cold saline solution from the blood within 35-50 with after decapitate was frozen in liquid nitrogen where they were stored until the time of the study.

Retrieved from liquid nitrogen the liver tissue immediately finely crushed blade and homogenized in microspherical tissues RT-2 (Russia) with pastikova homogenizer at a speed of 3000 Rev/min Homogenization was made in 0.1 M potassium phosphate buffer (pH 7.4) at a temperature of 0°ratio of tissue: buffer 1:9.

After processing, the tissue of the homogenate was immediately sampled to determine the concentration of malondialdehyde (MDA). At this time, since the thawing of tissues before sampling did not exceed 60 C. Then, for the deposition of organelles and microsomes, the remaining homogenate was subjected to centrifugation (10000 g, the temperature is +2°C) for 5 h in a centrifuge L8-M (Beckman, USA). The supernatant was used for estimation of the intensity of SRO and AOS activity.

The content of malondialdehyde, one of the secondary LPO products was determined by the method of [34]. The method is based on the formation of pink trimethylboron complex when interacting malondialdehyde 2-thiobarbituric acid at high temperature in an acidic environment. The optical density of pink Chromogen were measured on the spectrophotometer at a wavelength of 532 nm. The amount of MDA was calculated using molar extinction coefficient of 1.56×105cm-1M-1. To estimate the intensity of SRO and AOS activity used one of the most sensitive methods of detection of hydroxyl and peroxy radicals in biological substrates - a method of registering the intensity of the induced biochemiluminescence [35].

The performance of the morphological composition of blood was calculated by standard methods.

As seen from the data in table 2, the tool-prototype does not have a significant impact on the lethal effect of irradiation, whereas the use of the claimed means the survival of irradiated animals was increased to 3.75 times and amounted to 45%.

Table 2

The influence of the proposed means of the prototype on the survival of the life expectancy of rats, irradiated at a dose of 7.5 G
Used toolThe number of animals
onlyOf them survived
Abs.%
Irradiation (control)32012±8
Tool-prototype3667±6
The inventive tool361344±81,2
1- p<0.05 compared with the irradiated control;

2- p<0.05 compared with vehicle-prototype.

Unlike tools of the prototype, the use of the proposed drug was accompanied by a distinct positive dynamics of postradiation violations of the morphological composition of peripheral blood. As seen from the data in table 3, the irradiation as control and treated with the use of the prototype rats caused the development of deep radiation between 3 and 15 days after radiation exposure (maximum reduction at 7 days was more than 90% from baseline). At the same time in rats that were administered the inventive tool is, in all the studied period, the number of leukocytes was significantly higher than in the control and treated with skin is by means of the prototype.

Table 3

The influence of the proposed means and means the prototype for the maintenance of leukocytes in the peripheral blood of rats
Used toolThe content of cells, n×109/l
before irradiationafter exposure through
3 days7 days10 days15 days
Irradiation (control)10,3±0,54,2±0,20,9±0,11,4±0,11,4±0,1
Tool-prototype10,6±0,54,4±0,21,1±0,11,6±0,21,5±0,1
The inventive tool10,8±0,45,3±0,11,21,9±0,11,22,5±0,11,23,4±0,11,2
1- p<0.05 compared with the irradiated control;

2- p<0.05 compared with vehicle-prototype.

In irradiated rats already early after radiation exposure was developed typical symptoms of oxidative stress in blood and liver was increased MDA content (table 4.), in what was increasing the intensity of the SRO and decreased activity of AOS (table 5, 6).

Studies have shown that the insertion of the prototype did not affect the dynamics of accumulation of the metabolite FLOOR in blood plasma and liver tissue did not reduce postradiation the activation of free radical oxidation, remained low activity of antioxidant systems. Unlike tools of the prototype, pathcomponents antioxidant complex contributed to a significant decrease in the concentration of MDA in plasma and especially in the liver tissue of irradiated animals. As can be seen from the data presented in table 4, in all the studied period the contents of this product FLOOR in the liver of animals treated with the use of the proposed drug, was 1.5-2.0 times lower than in the control group or the treatment using the prototype.

Under the influence of the proposed drug in the first day after irradiation normalized intensity SRO in plasma and liver tissue, increased activity of AOS (table 5, 6). Thus, according to the obtained results, the antioxidant properties of the proposed drug was significantly higher in comparison with the tool prototype that was caused, apparently, by the inclusion of the complex of low molecular weight thiols (unithiol), reducing free radicals, and stabilizing enzymatic antioxidants and selenium compounds necessary for when abilitati of metals of variable valence and functioning of selenium-dependent glutathione peroxidase [15].

High antioxidant activity and results in a more pronounced compared to vehicle-prototype, radioprotective properties of the proposed drug.

Thus, studies have shown that the claimed means of representing the antioxidant complex of vitamins a, C, E, unithiol and of sodium Selenite, has a pronounced therapeutic effect when irradiated at doses close to the minimum absolutely lethal. Radioprotective effect of the complex appears to increase the survival of animals, accelerated recovery of leucopoiesis and weakening manifestations of oxidative stress.

Table 4

The influence of the proposed funds from the prototype to the concentration of MDA in plasma and liver tissue of rats irradiated at a dose of 7.5 G
Used toolThe concentration of MDA:
in plasma, mmol/lin liver tissue, nmol/g
before irradiationafter exposure throughbefore irradiationafter exposure through
7 h25 h49 h7 h25 h 49 h
Irradiation (control)3,28±0,063,79±0,0913,66±0,11113,97±0,09120,1±1,223,8±0,4127,8±1,3120,2±1,0
Tool-prototype3,80±0,1013,75±0,1213,81±0,11123,6±0,3124,2±1,1120,1±1,0
The inventive toolof 3.46±0,122,33,23±0,132,32,88±0,182,313,2±0,81,2,313,6±0,71,2,313,4±0,41,2,3
1- p<0.05 compared with the level before irradiation;

2- p<0.05 compared with the irradiated control;

3- p<0.05 compared with vehicle-prototype

Table 5

The influence of the proposed means of the prototype on the intensity of the SRO and the activity of antioxidative system in the blood plasma of rats irradiated at a dose of 7.5 G
Used toolControlled parameter:
intensive the industry SRO, mVthe activity of AOS, the river. Ed.
before irradiationafter exposure throughbefore irradiationafter exposure through
7 h25 h49 h7 h25 h49 h
Irradiation (control)6,13±0,056,74±0,2116,86±0,1816,63±0,1517,66±0,117,39±0,2017,21±0,1817,28±0,151
Tool-prototype6,68±0,131of 6.71±0,1916,51±0,141of 7.48±0,227,29±0,1317,31±0,121
The inventive toolare 5.36±0,161,2,35,69±0,212,36,29±0,197,71±0,197,80±0,112,37,76±0,151,2,3
1- p<0.05 compared with the level before irradiation;

2- p<0.05 compared with the irradiated control,

3- p<0.05 compared with vehicle-prototype

tr>
Table 6

The influence of the proposed means of the prototype on the intensity of the SRO and the activity of antioxidant in the liver tissue of rats irradiated at a dose of 7.5 G
Used

tool
Controlled parameter:
the intensity of the SRO, mVthe activity of AOS, the river. Ed.
before irradiationafter exposure throughbefore irradiationafter exposure through
7 h25 h49 h7 h25 h49 h
Irradiation (control)5,63±0,126,92±0,1316,86±0,3216,67±0,1016,99±0,136,25±0,1314,45±0,1816,03±0,221
Tool-prototypefor 6.81±0,1816,40±0,2116,65±0,1716,21±0,1414,60±0,2216,15±0,211
The inventive tool5,64±0,232,35,15±0,182,35,46±0,222,3 6,30±0,2116,22±0,211,2,36,68±0,222,3
1- p<0.05 compared with the level before irradiation;

2- p<0.05 compared with the irradiated control;

3- p<0.05 compared with vehicle-prototype

The inventive product has no adverse toxic effects, no significant impact on the behavioral reactions of animals, haematopoiesis, the liver and kidneys.

The claimed invention satisfies the criterion of "novelty", as it was first demonstrated therapeutic efficacy of the antioxidant complex of vitamins a, E, C, unithiol and of sodium Selenite, radiation damage, radiation-induced in absolutely minimal lethal doses. On the effectiveness of the proposed remedy twice a tool prototype.

The claimed invention meets the criterion of "inventive step", since on the basis of available information, the possibility of using the developed pathcomponents antioxidant complex as a therapeutic agent in radiation damage is not obvious.

The compliance of the claimed invention, the criterion of "fitness for use" is confirmed by the examples and the fact that all the components of the coefficients of the recipes are officinal preparations produced by domestic industry [36-41].

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The tool early pathogenetic therapy of acute radiation sickness, including retinol acetate, alpha-tocopherol acetate, ascorbic acid, characterized in that it further comprises unithiol and sodium Selenite, and the components are taken in the following amount, mg/kg:

Retinol acetate7
Alpha-tocopherol acetate20
Ascorbic acid20
Unithiol50
Sodium Selenite0,1



 

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SUBSTANCE: claimed enterosorbent contains 45-80 mass % of polysaccharide and balance: boehmite. As polysaccharide enterosorbent contains chitosan or lignin, or microcrystalline cellulose. Enterosorbent of present invention is useful in excretion of toxic substances from organism, prophylaxis of heavy metal intoxication, etc.

EFFECT: enterosorbent with high adsorption ability in relation to heavy metal ions; application only one kind of polysaccharide.

2 cl, 1 ex, 3 tbl, 2 dwg

FIELD: medicine.

SUBSTANCE: claimed method includes blending of active base and auxiliary ingredients to form tablet corn, representing composition of sugar powder, monocrystalline cellulose, vinylpyrrolidone and calcium stearate; humidifying of obtained mixture; drying of obtained granules; dry granulation through granulator with standardized holes; pelletization of standardized granules to produce tablet corn; and coating. Mixture is humidified with 5-7 % starch mucilage in starch mucilage/humidifying mixture mass ratio of 1:25-30, wherein mixture is blending with starch mucilage for homogeneous distribution wet in whole mass.

EFFECT: tablets with increased hardness and enhanced pharmacological activity.

2 cl, 2 ex

FIELD: medicine, in particular veterinary.

SUBSTANCE: invention relates to method for treatment of cattle intoxicated with Cynoglossum officinalis containing in feed hey as admixture. Claimed method includes administration perorally of sorbent, namely healthy feed supplement containing in dose of aluminum silicate in dose of 0.15 g/kg of body mass; saccharose in dose of 0.5-1.0 g/kg of body mass; and beta-carotene comprising 10-20 mg/ml of active ingredient in dose of 2 ml 1 time per day; administration intramuscularly of 1 % riboflavin mononucleotide solution 3 ml; 1 % pyridoxine hydrochloride solution 1 time per day; tetravit in dose of 3 ml 2 time during treatment course with interval for 5 days, wherein total treatment course includes 10 days.

EFFECT: method with simultaneous antioxidant, antitoxic, metabolism stimulating, and liver function normalizing action.

2 ex, 3 tbl

FIELD: experimental medicine.

SUBSTANCE: in rabbits one should induce hepatic ischemia due to occlusion of hepato-duodenal ligament for 30 min. Moreover, reamberin should be introduced at the dosage of 35 ml/kg during the whole period of occlusion, and at the dosage of 5 ml/kg for 10 min after removing the above-mentioned occlusion. The method provides preventing the death of experimental animals in case of ischemic exposure being harmful for this type of animals.

EFFECT: higher efficiency of protection.

2 tbl

FIELD: medicine.

SUBSTANCE: invention relates to application of pectin solution as detergent for skin and hair in radioactive and environmentally hazardous regions. Pectin containing in solution due to chelating action bonds to heavy metal ions to form stable compounds (micelles) having very large size and prevents transferring thereof trough transdermal barrier. Formed micells may be easily removed from human body.

EFFECT: new detergent for skin and hair useful in radioactive and environmentally hazardous regions.

2 ex, 5 tbl

FIELD: pharmacology.

SUBSTANCE: the present innovation deals with decreasing and/or blocking adhesion of pathogenic substances and organisms upon eucaryotic cells. The preparation suggested contains, at least, one carbohydrate at structural link of uronic acid at one of its ends. About 10 to 100% available terminal structural links of uronic acids of carbohydrates contain, moreover, a double bond which, in particular, is available between C4- and C5-atoms. The innovation broadens the number of antipathogenic means for eucaryotic cells.

EFFECT: higher efficiency.

17 cl, 13 ex, 1 tbl

FIELD: veterinary science.

SUBSTANCE: the suggested composition contains a certain salt of calcium hypochlorite as an oxidizing agent and, additionally, zeolite as an adsorbent at the following ratio of components, weight%: a certain salt of calcium hypochlorite 30.0-70.0, zeolite - the rest. Application of the composition suggested provides neutralization of animal skin under any environmental conditions, both at plus and minus environmental temperature values.

EFFECT: higher efficiency of application.

6 ex, 1 tbl

FIELD: herb therapy.

SUBSTANCE: method comprises laboratory analysis of human body fluids, reveal of excess/deficit of macro and trace minerals followed by selection of appropriate drugs. In case of excess of macro and trace minerals, herb gatherings favoring neutralization and excretion of excess trace minerals and antagonists with maximum concentration of the latter are used. In case of deficit of macro and trace minerals in the human body, herb gatherings having elevated concentrations of such minerals and enhancing assimilation thereof with coefficients of biological accumulation above 1.0 are used. Herb gatherings are administered thrice a day 15-30 min before meal in the form of aqueous extract prepared in the basis of 10-30 g of dry vegetable stock per 150-300 ml water. Administration of herb gathering is combined with administration of vitamins activating biological transformation of antagonists or deficient macro or trace minerals in human body.

EFFECT: normalized trans mineral homeostasis due to high bioavailability and assimilability of developed herb complexes.

7 cl, 5 ex

FIELD: medicine, medicinal biochemistry, pharmaceutical technology.

SUBSTANCE: invention proposes the composite that comprises complex of vitamins D3, B6, C, K and calcium salts, citric acid, lactose and sorbitol as the special supplement, and lubricating agent and correcting agent for taste and/or odor. The method for preparing the composite involves mixing the above said components and if necessary the following tableting process of the prepared mixture. The new composite shows stability of quality indices in the store process being among them index "the content of vitamin D3" that provides the fitness time above 2 years and absence of by-side adverse toxic effect that is typical for destruction products of active components.

EFFECT: improved preparing method, improved and valuable properties of composite.

7 cl, 1 tbl, 4 ex

FIELD: pharmaceutical industry.

SUBSTANCE: invention relates to creation of therapeutical and prophylactic balsams, which can be used as generally restorative and tonic agents to increase resistance of organism to reduced levels of atmosphere oxygen (increase of hypoxia resistance) as well as to prevention and correction of functional disorders associated with stress and exhausting environmental factors affecting organism. Invention proposes two variants of therapeutical-prophylactic balsam producing generally restorative and tonic effects, each containing vegetable oils, vitamins, amino acids, hepatic implant cells (Hepatosan), adaptogen (Astragerm), antioxidant, odorant, and thickener.

EFFECT: achieved normalization of vitality in exhausted organism.

6 cl

FIELD: medicine, pharmacy.

SUBSTANCE: invention relates to a soluble, carbonated, anti-inflammatory, analgesic and antipyretic agent as a tablet. Agent comprises acetylsalicylic acid, caffeine, ascorbic acid, paracetamol, basic component, acidic component and accessory substances. Invention provides the possibility of rapid release of the preparation in blood, decreasing time for undesirable contact of the preparation with stomach mucosa and its high activity.

EFFECT: valuable medicinal and pharmaceutical properties of agent.

6 cl, 6 tbl, 11 ex

FIELD: pharmaceutical industry.

SUBSTANCE: claimed complex contains vitamins such as B1, B2, B3 (nicotineamide), B5 (calcium pantothenate), B6, B9 (folic acid), B12, A, C, D3, E, beta-carotene, lycopitin, and minerals such as magnesium, copper, iodine, iron, molybdenum, selenium, calcium, phosphorus, chromium, zinc, and manganese. Complex is separated into two solid formulations taken at different time. The first solid formulations contains B1, B2, B3 (nicotineamide), B5 (calcium pantothenate), B6, B9 (folic acid) and minerals such as magnesium, copper, iodine, iron, molybdenum in specific component ratio; the second one contains A, C, D3, E, beta-carotene, lycopitin, and minerals such as selenium, calcium, phosphorus, chromium, zinc, and manganese in specific component ratio.

EFFECT: vitamin-and-mineral complex of improved antioxidant properties.

6 cl, 1 ex

Healing agent // 2271814

FIELD: medicine, pharmacy.

SUBSTANCE: invention represents a healing agent used for close and treatment of suppurative-necrotic and infected wounds, burns, trophic ulcers. Proposed composition comprises the following components taken in the required ratio: water-soluble chitosan of natural origin, polyalkyleneguanides phosphopag and biopag, proteolytic enzymes pepsin and collagenases, ascorbic acid and anesthetic agent. Agent shows the combined wound-healing, bacteriostatic and immunostimulating properties, it stimulates regenerative-reparative processes and accelerates growth of wound healthy cells. Also, agent decreases number of little use and/or expensive components used in preparing wound-healing agents.

EFFECT: improved and valuable medicinal properties of agent.

7 cl, 2 tbl, 10 ex

FIELD: medicine, oncology, biology.

SUBSTANCE: method involves per os administration of the preparation "oligokhit activ" to a cancer carrier in the dose 20-24 mg/kg of weight for 7 days, not less. Method provides elimination of dystrophic changes in hepatocytes caused by metabolic disturbances in body of a cancer carrier, to improve proliferative activity of hepatocytes and to recover microcirculation.

EFFECT: improved correction method.

1 tbl, 1 ex

FIELD: medicine, pharmacy.

SUBSTANCE: invention relates to the development of medicines used for enhancing resistance of humans and animals to hypothermia. Agent for enhancing resistance of humans and animals to hypothermia comprises nicotinic acid, glutamic acid, riboflavin, ascorbic acid, uniquinone, succinic acid, polyethylene oxide and water taken in the definite ratio. Also, invention proposes a method for enhancing resistance of humans and animals to hypothermia involving administration of the abovementioned agent 30 min before works associated with hypothermia and after its termination for activation of vital essential biochemical processes. Invention provides enhancing resistance to overcooling under extreme conditions and using in surgery practice in cooling organs (in operations in heart and brain).

EFFECT: enhanced effectiveness and valuable medicinal properties of agent.

2 cl, 2 tbl, 1 dwg, 2 ex

FIELD: medicine.

SUBSTANCE: invention relates to a composition used in prophylaxis and treatment of diseases or pathological states associated with destruction of extracellular matrix. Proposed composition can be used in treatment and prophylaxis of destructive diseases, in particular, arteriosclerosis, cancer, infectious and other inflammatory diseases and other diseases. The composition comprises preferable compounds comprising lysine, proline, ascorbate and their derivatives, and synthetic analogues, and vitamins, provitamins and trace elements also. Invention provides reducing destruction and stimulates collagen synthesis simultaneously that prevents the development of the abovementioned diseases.

EFFECT: valuable medicinal properties of composition.

10 cl, 2 tbl, 4 dwg

FIELD: medicine, toxicology, resuscitation.

SUBSTANCE: one should introduce preparations due to transmembranous wound dialysis: from the 1st to the 3d d after chemical burn one should introduce dialyzate of the following composition: solution of rheopolyglukin 100 ml, 2%-lidocaine 4 ml, 1%-dioxidine 10 ml, dexamethasone 8 mg, and from the 4th to the 7th d of the disease mentioned it is useful to introduce dialyzate including: solution of rheopolyglukin 100 ml, 1%-dioxidine 10 ml, 5%-ascorbic acid 5 ml; moreover, transmembranous wound dialysis should be carried out once daily. The present innovation enables to conduct local treatment of chemical esophageal burns due to taking into consideration the phases of wound process of burn trauma that, in its turn, favors for alleviating and accelerating clinical flow of the disease in question and preventing the development of complications.

EFFECT: higher efficiency of therapy.

1 cl, 2 ex

FIELD: medicine, pediatrics.

SUBSTANCE: the present innovation deals with antenatal prophylaxis of transitive myocardial dysfunction in neonatals before their birth. One should introduce limontar daily per 1 tablet for pregnant women at the period of 35-36 wk gestation additionally to conventional therapy. It is necessary to conduct one course of prophylaxis for 14 d. The innovation provides normalization of myocardial contractile function in neonatals and prophylaxis in development of side effects caused by the preparation introduced.

EFFECT: higher efficiency of prophylaxis.

3 ex, 1 tbl

FIELD: pharmaceutical industry.

SUBSTANCE: invention relates to creation of therapeutical and prophylactic balsams, which can be used as generally restorative and tonic agents to increase resistance of organism to reduced levels of atmosphere oxygen (increase of hypoxia resistance) as well as to prevention and correction of functional disorders associated with stress and exhausting environmental factors affecting organism. Invention proposes two variants of therapeutical-prophylactic balsam producing generally restorative and tonic effects, each containing vegetable oils, vitamins, amino acids, hepatic implant cells (Hepatosan), adaptogen (Astragerm), antioxidant, odorant, and thickener.

EFFECT: achieved normalization of vitality in exhausted organism.

6 cl

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