Method for predicting influence of hearing-aid insert usage upon postoperative middle ear cavity state before carrying out electroacoustic hearing correction

FIELD: medicine.

SUBSTANCE: method involves determining acoustic meatus bone part skin immunocytogram type. To do it, total number of cells and cell composition percent proportions are determined in smears taken from acoustic meatus bone part epidermis surface. Favorable influence of the insert upon postoperative middle ear cavity state is predicted, if neutrophil content is not greater than 10% in epithelial immunocytogram type cases. Unfavorable influence with high probability of chronic otitis recurrence is predicted, if neutrophil content is greater than 50% in neutrophilic immunocytogram type cases.

EFFECT: high accuracy of prognosis.

 

The invention relates to medicine, namely to otolaryngology, and can be used to predict the results of the impact liner of a hearing aid in postoperative middle ear cavity before electroacoustic correction of hearing.

Radical sanitizing operation on the middle ear is used for the treatment of patients with chronic suppurative otitis media. Despite improvements in surgical treatment of chronic suppurative otitis media in many patients fail to improve hearing surgical treatments (Sniecinski, Showjapanese, Irz, Abolition. The experience of hearing by shielding window of the cochlea when the effects of chronic suppurative otitis media.//Journal of the ear, nose and throat diseases. - 1980. No. 1. - P.61-63). These patients have after the operation to use conventional hearing AIDS.

An important part of a hearing aid is an individual an earmold. It provides a sound transmission in the ear of the patient through free, fix a hearing aid in the ear and connects the residual volume of the ear canal with the external environment by means of additional or bell section Venta (H.Dillon. Hearing Aids. 2001. - Thieme: New York. - P.117-118).

There is a method of evaluating the effect of the liner on subjective criteria: the shape and size of the contribution of the Sha should not cause unpleasant or painful sensations in the patient (Svjazana, Viewdb, Samegreloshi, Segvreason, UMIST, Iverieli Sluhoprotezirovanie. Manual for doctors. - SPb.: LLC "RIA-AMI", 2003. - p.9). However, this method does not allow to predict the effect of a liner with long-term use in patients after radical surgery on the middle ear and the possibility of adverse effects.

The authors in scientific medical and patent literature not found a method for predicting the results of the use of the liner of a hearing aid in postoperative middle ear cavity before electroacoustic correction of hearing patients undergoing radical surgery.

The technical result - obtaining criteria predict the results of the use of the liner by assessing the condition of the epithelial lining of postoperative middle ear cavity according to immunology before choosing a hearing aid.

The proposed method estimates the epithelial lining of postoperative middle ear cavity before electroacoustic hearing correction is as follows. The biomaterial is taken from postoperative cavity before electroacoustic correction of hearing ear probe with a sterile cotton swab soaked in warm saline solution by tangential motion and transferred on dry fat-free degree is La. Obtained swabs dried in air, fixed in 96% ethanol for 1 min, stained according to the method Romanovsky - Institute. After drying, the sample is examined under low magnification (× 150) to determine the number of cells in the smear, and then under immersion under higher magnification (× 630). In smears determine the total number of cells (lymphocytosis) and cellular composition in percentage.

Conducted immunocytological examination of smears - perepechko with skin bone of the Department of external ear canal 12 patients with a cavity after radical sanitizing operations on the middle ear before electroacoustic correction of hearing and in 22 healthy persons All patients of the experimental group according to otoscopy had epidermization cavity and no visible signs of inflammation.

In smears - reprints from the surface of the epidermis bone of the Department of external ear canal in practically healthy persons (n=22) revealed a small number of cells, on average 151,95±36,77; the cellular structure was presented to the keratinized epithelial cells (scales) - 97,05±0,86%; squamous cells - 2,70±0,87% and isolated neutrophils 0,25±0,10%.

In the group of patients after sanitizing radical surgery on the middle ear to the prosthesis in the analysis of the results revealed that 7 patients in smears-is repicate lymphocytosis was 126,77± 44,60. In the assessment of cellular composition in all patients of this group were mainly dominated by keratinized epithelial cells, and the content of neutrophils was from 1 to 10%.

4 patients lymphocytosis was 2237,5±1104,42. In the assessment of cellular composition cytologically detected signs of inflammation with a characteristic increase in the percentage of neutrophils from 51% to 94%, while when otoscopy clinically obvious signs of inflammation were observed.

In 1 patient was observed mixed immunotherapy with almost the same percentage of neutrophils (37%) and keratinized epithelial cells (36%).

Patients of the first group (the content of neutrophils to 10%) in the future (1-6 months) there was good clinical effect of the use of the liner of a hearing aid. With long-term use of the liner (more than 8 hours per day) patients noted the lack of moisture, irritation of the skin of the ear canal, when otoscopy at 7, 21 and 60 days were not detected signs of skin inflammation bone Department of the ear canal.

All patients of the second group (the content of neutrophils more than 50%) complained of a feeling of moisture when using insert more than 8 hours per day, sometimes itching in the ear. 1 patient 4 was noted gnetaceae for the 21-day application of the liner, during which he was forced to abandon used what I was hearing.

Patients with mixed type immunocytometry when using insert more than 8 hours per day was celebrated periodic feeling of stuffiness ear, when otoscopy on day 21 revealed moderate redness of the ear canal.

Thus, epithelial type immunocytometry skin bone Department of the ear canal with a low percentage of neutrophils (less than 10%) is a favorable prognostic sign, and neutrophilic type immunocytoma (neutrophils constitute more than 50% of all cells) - negative, high risk of developing complications from the use of the liner of a hearing aid. Mixed immunotherapy (neutrophils is from 11 to 50%) - forecast doubtful.

Example 1.

Kozlowski, S. (outpatient map No. 1021), 59.

Diagnosis: Bilateral chronic suppurative otitis media. Status after radical sanitizing operations on the right ear (1978). Left-sided chronic tubotympanic otitis media, remission stage. Bilateral mixed hearing loss 3 degrees to the right, 4 degrees to the left.

Otoscopes painting cavity after radical surgery was characterized by the absence of visible signs of inflammation, especially of the oral medium, epidermization more than 80% of the area, low spur", no remnants of the tympanic membrane and auditory KOs is the eyeglasses. A history of sickness absence in the past 12 years.

Immunocytological picture smear-reprint with skin bone Department of the ear canal to electroacoustic correction of hearing were characterized by low lymphocytosis (150), smear prevailed keratinized epithelial cells (scales) - 81%, was present in a small number of cells of a flat epithelium - 18% and isolated neutrophils - 1%.

Patients performed a selection of hearing aid AM 34 and made individual acrylic ear liner with additional ventom 1,0 mm

7, 21 and 60 days of using individual insert hearing aid (more than 8 hours per day) otoscopes picture was characterized by the absence of visible signs of inflammation. The patient complained of a feeling of moisture, itching, pain when using liner and noted the good results of the application of the liner. 6 months after applying liner postoperative cavity was epidermization absence (according to otoscopy and Cytology) signs of inflammation.

Example 2.

Kajgorodtseva I.N. (outpatient map No. 1380), 44.

Diagnosis: Bilateral chronic suppurative otitis media. Status after radical sanitizing operations on the left ear (1980). Status after radical sanitizing operations on the right ear (2001). Bilateral smarandachely 3 degrees on both sides.

Otoscopes painting cavity after radical surgery on the right was characterized by the absence of visible signs of inflammation, especially cavity - small size, epidermization more than 70% of the area, the presence of residues of the eardrum. A history of sickness absence within 3 years.

Immunocytological picture smear-reprint with skin bone Department of the right ear canal to electroacoustic correction of hearing were characterized by low lymphocytosis (126), smear prevailed keratinized epithelial cells (scales) - 86%, of the cells of a flat epithelium - 5% and neutrophils amounted to 9%.

Made selection of a hearing aid INFINITI PRO and made individual acrylic ear liner with additional ventom 1,0 mm

7, 21 and 60 days of using individual insert hearing aid (more than 8 hours per day) otoscopes picture was characterized by the absence of visible signs of inflammation. The patient complained of stuffiness, itching, pain when using liner and noted the good results of the application of the liner. 6 months after applying liner postoperative cavity was epidermization absence (according to otoscopy and Cytology) signs of inflammation.

Example 3.

Bailamos P.P. (outpatient map No. 2421), 43.

Diagnosis: Bilateral XP the technical suppurative otitis media. Status after radical sanitizing operations on the right ear (1974). Left-sided chronic total otitis media, remission stage. Bilateral mixed hearing loss 3 degrees on both sides.

Otoscopes painting cavity after radical surgery was characterized by the absence of visible signs of inflammation, especially cavity - large, epidermization about 70% of the area, the absence of residues of the auditory ossicles. A history of sickness absence within 2 years.

Immunocytological picture smear-reprint with skin bone Department of the ear canal to electroacoustic correction hearing was characterized by high lymphocytosis (3000), in a smear was dominated by neutrophils - 90%, was present in a small number of cells of a flat epithelium - 2%, lymphocytes - 5% and macrophages - 3%

Patients performed a selection of hearing aid Swing S1 and manufactured individual acrylic ear liner with additional ventom 1,0 mm

On the 7th day use liner hearing aid (more than 8 hours per day) otoscopes picture was characterized by signs of local inflammation of the skin of the ear canal and intertemporally cavity. On day 21, the patient complained of a feeling of moisture when using a liner, and discharge from the ear. The use of a hearing aid was temporarily when it is recovered, the patient received medical therapy with daily toilet of the cavity after surgery and local antibiotic therapy taking into account the sensitivity of microflora, physical. After treatment, the patient resumed hearing AIDS and noted satisfactory results only in the short-term use (no more than 4 hours per day).

Example 4.

Calamine L.G. (outpatient map No. 1359), 29.

Diagnosis: Bilateral chronic suppurative otitis media. Status after radical sanitizing operations on the right ear (1995). Status after radical sanitizing operations on the left ear (1999). Right-sided deafness. Left-sided mixed hearing loss 4 degrees.

Otoscopes painting cavity after radical surgery on the left was characterized by the absence of visible signs of inflammation, especially cavity - large, epidermization about 70% of the area, the absence of residues of the auditory ossicles and the tympanic membrane. A history of sickness absence within 3 years.

Immunocytological picture smear-reprint with skin bone Department of the ear canal to electroacoustic correction hearing was characterized by high lymphocytosis (2100), in a smear was dominated by neutrophils - 52%, keratinized epithelial cells was 40%, was present in the small is the first number of cells of a flat epithelium - 2%, lymphocytes - 4% and macrophages - 2%.

The patient made selection of hearing AIDS SPECTRA PP+ and made individual acrylic ear liner with additional ventom 1,0 mm

On day 21 the use of the liner of a hearing aid (more than 8 hours per day), the patient complained of a feeling of stuffiness and humidity after use of the liner. Otoscopes picture on the 21st day of use liner was characterized by signs of local inflammation of the epithelial lining of the postoperative cavity, tympanal part of the cavity appeared scarce Muco-purulent discharge. The use of a hearing aid was suspended, the patient held medical therapy.

A method for predicting the impact of the use of the liner hearing on the state of the postoperative cavity of the middle ear before electroacoustic hearing correction, characterized in that determine the type of immunotherapy skin bone Department of the ear canal, which smears the reprints from the surface of the epidermis bone of the Department of external ear canal to determine the total number of cells and cellular composition in percentage and in the epithelial type immunocytometry containing neutrophils not more than 10% predict a beneficial effect of the liner on the condition the postoperative cavity, and when neutrophilic type immunocytometry containing neutrophils more than 50% of adverse effects with high probability of relapse of chronic otitis media.



 

Same patents:

FIELD: animal science, cattle science.

SUBSTANCE: one should study urine for the content of malonic dianhydride (MDA), where at its value being above 0.2 units of optic densit5y it is possible to diagnose subclinical endometritis. The innovation suggested provides the chance to diagnose endometritis rather quickly at high accuracy and objectivity at earlier stage of the above-mentioned disease (subclinical endometritis) without any use of expensive reagents and equipment.

EFFECT: higher accuracy and efficiency of diagnostics.

3 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: method involves determining separated glycogen content in adportal hepatocytes adjacent to the central vein and taking intermediate position between said zones. Reactivity index is calculated as IR=A/B, where A is the hepatocytes percent having substrate in the amount exceeding 75% from cytoplasm area adjacent to the central vein and hepatocytes occupying intermediate position between the zones, B is the hepatocytes percent having substrate in the amount exceeding 75% from cytoplasm area adjacent to portal tract. The RI value being equal to or less than 0.37, stress-training response is to be determined. RI value being greater than 0.7, activation response is determined.

EFFECT: high accuracy of the method.

1 tbl

FIELD: veterinary medicine.

SUBSTANCE: method involves analyzing milk for ceruloplasmin content. The value being found greater than 0.4 units of optical density, mastitis diagnosis is to be set.

EFFECT: high accuracy of method applicable at early stage of disease development.

1 tbl

FIELD: medicine.

SUBSTANCE: method involves determining quantitative ratio between pyruvic acid and lactate concentration in saliva. The value being equal to or greater than 0.48, low hypoxia compensation chances requiring medicamentous intervention are to be predicted. The value being less than 0.48, adequate hypoxia compensation response is to be predicted without medicamentous intervention being required.

EFFECT: high accuracy of noninvasive method; wide range of applications in examining cardiovascular system condition of young people to be called to military service.

2 tbl

FIELD: medicine.

SUBSTANCE: method involves determining low and medium molecular mass substances availability in plasma, on/in erythrocytes, in urine, effective albumin concentration in blood plasma, antiprotease plasma activity index, birefringent crystal number in citrated plasma with proteolysis inhibitor and orthophenanthroline test. Next, three integral characteristics, showing level of endogenous intoxication syndrome gravity as light, moderate and severe, are to be calculated. The endogenous intoxication syndrome gravity degree is determined from which of the indices under measurement reaches maximum value.

EFFECT: higher diagnosis accuracy.

FIELD: medicine.

SUBSTANCE: method involves making extraction with dimethyl sulfoxide mixed with water in 3:1 proportion in retort having backflow condenser on water bath during 1 h. The produced extract is filtered. The filtrate is chromatographically studied first in 5 cm high and 0.7 cm diameter columns with alumina and then in thin sorbent layer solvent system containing condensed ammonium and alcohol taken in 1:4 proportion. The eluate is subjected to spectrophotometric analysis at 278±2 nm. Oxymethyl uracyl quantity is determined from formula C=D x b/(E1%cvxa), where C is the substance concentration in %, D is the optical density, b is the dilution rate, E1%cv is the specific absorption index, a is the exact weighing in g.

EFFECT: high accuracy of blood oxymethyl uracyl control in treatment course.

FIELD: medicine.

SUBSTANCE: method involves determining α2-macroglobulin activity level in prostate secretion sample. Its activity level belonging to an interval from 0.1 to 0.2 IU/ml, noninflammatory chronic nonbacterial prostatitis is to be diagnosed. The value being equal to or greater than 0.2 IU/ml, inflammatory chronic nonbacterial prostatitis is to be diagnosed.

EFFECT: high accuracy of diagnosis.

FIELD: medicine.

SUBSTANCE: method involves calculating coefficient describing ratio of polymorphous nuclear leukocyte number having single-stranded RNA to and polymorphous nuclear leukocyte number of double-helical DNA before (αk) and after (α0) treatment with endotoxin in blood sample smears stained with acrydine orange. αk to α0 correlation index is determined. The value being equal to or greater than 1, lingering clinical course of acute intestinal infection is to be predicted.

EFFECT: high prognosis accuracy.

FIELD: medicine and biochemistry.

SUBSTANCE: claimed method includes blood lymphocyte treatment with labeled fluorescein isothiocyanate of pea lectin isoform obtained by extraction of milled pea seeds with sodium chloride solution, purifying by undercooling, dialysis and isoform isolating by column chromatography on Sephadex, and elution with glucose-containing solution, and when number of labeled lymphocyte is lower than 50 % in contrast to normal one Ehrlich's carcinoma is diagnosed.

EFFECT: diagnosis method of increased specificity.

1 tbl, 3 dwg

FIELD: biochemistry.

SUBSTANCE: invention relates to method for biological material sample analysis in biological or immunological tests, wherein in sample treatment process coloring is observed, and color darkening is correlated with amount of tested substance. At least one color characteristic, such as coloring angle, chromaticity, darkening, and obtained color brightness is measured. Method of present invention is useful in analysis of lung, throat, cervix uteri or spermatic liquid mucilage for diagnosis of cancerous and precancerous conditions.

EFFECT: enhanced assortment of agents for diagnosis of cancerous and precancerous conditions.

14 cl, 4 dwg

FIELD: medicine, laboratory diagnostics.

SUBSTANCE: for the purpose of predicting infectious mononucleosis (IM) flow in children one should perform sampling from fauces mucosa to dissolve it at 1:1 ratio with physiological solution and keep in diagnostic cell for about 20-24 h. Then it should be microscoped in polarization light and at detecting branched morphostructures of confocal domains and anisotropic structures in the form of filaments followed by predicting favourable IM flow, and at detecting stratified confocal domains with dense black deposit and anisotropic structures as dense netting of filaments and spherulites it is possible to predict unfavorable IM flow in children. The application of the present innovation enables to carry out significant IM flow prediction in short terms with available technique.

EFFECT: higher efficiency.

2 dwg, 2 ex

FIELD: veterinary biochemistry, biochemistry of bony tissue.

SUBSTANCE: the present innovation deals with evaluating the activity of osteogenesis due to carrying out biochemical blood testing in the course of which it is necessary to detect the levels of total calcium, inorganic phosphate, magnesium and chlorine content (C) in blood serum during regeneration of diaphysial fractures of tubular bones without phenomenon of distraction to calculate the index of electrolytes (IE) according to the following formula: IE = CCa CMg CCl/CPO4. At its value being above 150 (up to250 and more) one should conclude upon catabolic phase, and at its value being below 150 (up to 110 and less) - anabolic phase of osteogenesis should be stated upon.

EFFECT: higher efficiency of control.

1 ex, 2 tbl

FIELD: medicine, physiology.

SUBSTANCE: the suggested method for isolating cardiomyocytes out of the heart of mature rat due to cardiac perfusion for 40 min with the mixture of enzymes as collagenase and pronase at concentration of 0.2 mg/ml and 0.1 mg/ml, correspondingly, enables to efficiently divide myocardium into separate, structurally normal and viable cells. Isolated cardiomyocytes of rats could be applied in trials directed to study the alteration in parameters of intra-cellular homeostasis in case of cardiovascular pathologies. The innovation provides decreased damaging impact upon cardiomyocytes due to simplifying the method applied.

EFFECT: higher efficiency.

4 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: method involves culturing microorganisms in liquid nutrient medium, separating supernatant from microbial cells by centrifuging. Control is concurrently prepared as liquid nutrient medium. Then, each sample is treated with chloroform and separating supernatant from chloroform by centrifuging. Next to it, Staphylococcus aureus test culture is added to case and control samples, incubated, test culture cells are separated by centrifuging and microbial suspension is prepared from them. Hydrogen peroxide solution is added to each sample and they are incubated. The test culture cells are separated by centrifuging, nutrient medium is added and incubated. Optical density of grown-up cultures is measured in case and control samples and test culture biomass live weight gain in case sample is determined from formula P=[(ODCS - ODC ]/ ODC] *100%, where P is the biomass liveweight gain in case sample compared to control; ODC is the optical density of control test culture; ODCS is the optical density of case test culture. Then, conclusions are drawn if protective activity microorganisms are available in Fenton effect by interpreting test culture biomass live weight gain data in case sample.

EFFECT: wide range of functional applications in studying microbial cell physiology in medium containing small quantities of hydrogen peroxide.

1 dwg, 2 tbl

FIELD: medicine.

SUBSTANCE: method involves carrying out bacteriological bioptate examination. The bioptate is taken from stomach stump. Peptostreptococcus spp. and/or Lactobacillus spp., and/or Staphylococcus spp., and/or Sarcina ventriculi in the amount not exceeding 103-104 CFU/g (colony-forming unit, CFU) being predominated in the bioptate, I-order micro-biocenosis disorder is evaluated. Escherichia coli and/or Bacteroides spp., and/or Enterococcus faecalis being found in the amount of 103 CFU/g and Streptococcus spp. and/or Staphylococcus spp. and/or Peptostreptococcus spp. being optionally detected in the amount of 103-105 CFU/g, micro-biocenosis disorder is evaluated as one of II-order. Escherichia coli and/or Bacteroides spp., and/or Enterococcus faecalis and/or Enterococcus faecium and/or Candida spp. being found in the amount of 104-107 CFU/g, micro-biocenosis disorder is marked as III-order.

EFFECT: high reliability and information capacity of the method.

FIELD: medicine.

SUBSTANCE: method involves carrying out bacteriological examination of scrape sample taken from injured tongue zone for detecting microbial infection and oral moniliasis and additionally determining norepinephrine and histamine content in oral liquid using liquid chromatography techniques. Examination results are interpreted in terms of benign migratory glossitis forms. Norepinephrine concentration being 2.37±0.64 ng/l as high, neurogenous form is to be diagnosed. Histamine concentration growing to 0.94±0.13 ng/l, allergic form is to be diagnosed. Plenty of coccal and diplococcal flora being found, dysbiotic form is diagnosed. Blast fungi of Candida type having rounded blastospores and large quantities of young and mature forms of pseudomycelium being detected, candidal form is to be diagnosed.

EFFECT: accelerated and accurate method for determining etiological provocation factor and glossitis form.

FIELD: medicine.

SUBSTANCE: method involves determining triiodothyronine concentration in blood sample taken from ulnar vein before and after hyperbaric oxygenation therapy session. The concentration increasing after the session when compared to triiodothyronine concentration before the session, brain contusion of moderate severity degree is to be diagnosed. No concentration growth being observed, heavy brain contusion is to be diagnosed.

EFFECT: high accuracy of diagnosis.

FIELD: medicine.

SUBSTANCE: method involves evaluating blood microcirculation parameters in urethral mucous membrane before tuberculin test and 48 h thereafter. The microcirculation parameters growing worse by 8-12% and higher, tuberculosis is to be diagnosed. The value remaining unchanged or their values not exceeding 8%, nonspecific urinary tract inflammation is to be diagnosed.

EFFECT: high accuracy of diagnosis.

2 tbl

FIELD: medicine.

SUBSTANCE: method involves recording iron-induced model system chemiluminescence and repeatedly recording chemiluminescence parameters under spermatoplasm being added in concordance with formula SAOA =(SPSMC/SMC)100%, where SAOA is the total antioxidant activity; SMC is the model system total; SPSMC is model system total + spermatoplasm. The ejaculate is estimated as fertile one when total antioxidant spermatoplasm activity is equal to 35-43%.

EFFECT: high accuracy of estimation.

FIELD: medical engineering.

SUBSTANCE: device has stabilized light source, reservoir for collecting blood enriched in blood platelets, photodetector, analog-to-digital converter and recording unit. The reservoir for enriching blood plasma with blood platelets is formed by two horizontal parallel-sided plates the lower of which has cylindrical cavity in the center. The cavity forms chamber when being engaged with the upper plate. The plates are rotatable in counter polar directions for stirring blood plasma without foreign bodies introduced into it and making strictly defined shear rate mode in the reservoir containing blood promoting spontaneous blood platelets aggregation.

EFFECT: high sensitivity in measuring spontaneous blood platelets aggregation.

2 cl, 4 dwg

FIELD: medicine, psychiatry.

SUBSTANCE: one should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.

EFFECT: more objective prediction of disease development.

3 ex

Up!