Device for producing digestive juice during chronical experiment

FIELD: medical and biological testing on animals.

SUBSTANCE: device for producing digestive juices in animals has main tube. End of tube is adapted for connection with gut; there is flange on the end. Elastic balloons are mounted at opposite sides if flange; balloons are provided with additional tubes for inflate them. Excision of segment of duodenum, where intestine clonus rhythm detector is disposed, is not required.

EFFECT: improved efficiency.

5 dwg


The invention relates to a device for biomedical research in experiments on animals.

In physiological studies of the digestive system in animals widely used different kinds fistulae tube. So, for example, to obtain the pancreatic juice in animals Dsicover [1] was proposed device consists of two cylindrical fistulina tubes. One of these tubes are inserted into the deformed segment of the duodenum with the mouth flowing into her pancreatic duct, and the other into the duodenum. The continuity of the intestinal tract restore by imposing interventionists "side-to-side. Outside the two tubes connect the rubber tube, resulting in a external bypass path for the pancreatic juice. During the experience fistulae tube sever and through which is inserted in a deformed segment of the duodenum, collect pancreatic juice.

The disadvantages of this device include the fact that its use is associated with the need to produce double full transverse transection of the duodenum, which is the sensor rhythm of intestinal contractions. This leads to contractile activity of the intestine. In addition, the probability of blockage of the winding workaround is obsessed intestine.

Well-known U-shaped intestinal-pancreatic fistula tube containing a hollow cylindrical body, which has two longitudinally spaced channel [2]. On the top of the case has two hollow cylindrical ridge located at an angle to each other, and each of them communicates with any one of the channels in the housing. Partition inside the housing between channels does not reach the bottom edge of the case and there is formed common to both channels space through which they communicated with each other. On the lower end of the housing has a screw-on cap. At the free ends of the prongs are flanges and adapted for connection with the intestine, and at the end of that process, which is intended for insertion into the duodenum, installed the valve.

The operation carried out with the purpose of installation in the gut this fistulas tube, main points similar to the above operation. From the duodenum excised segment of the mouth of the influent pancreatic duct, and the continuity of the intestinal tract restore by imposing interventionists "end to end". Then equipped with a valve process fistulas tube in a known manner is inserted into the duodenum, and the other process in deformed intestinal segment. Out of experience when closed fistulas t is ubke pancreatic juice through communicating with each other channels in the processes falls into the duodenum. During the experience with fistulas tube sbencivu cap, resulting in channels dismantled and it becomes possible to collect pancreatic juice.

One of the drawbacks of this device is that when it is necessary to excise a segment of the duodenum, which is the sensor rhythm of intestinal contractions. This leads to the violation of the motor activity of the intestine. Long and complex form of artificial path between transected intestinal segment and duodenum promotes stasis and infection of pancreatic juice. Valve, permanently inside the gut, quickly clogged the contents of the intestine and ceases to perform its functions. The consequence is hit intestinal chyme inside fistulas tube and clogging of its channels.

Fistulina tube on the author's certificate of the USSR No. 235241 considered as a prototype.

The objective of our proposal is to eliminate the above disadvantages of the known fistulas tube [2].

The problem is solved due to the fact that in the known apparatus for producing digestive juices, consisting of a main tube, the upper end of which is adapted for connection to the intestine and it has a flange on opposite sides of a flange mounted elastic cylinder is, provided with an additional tube for inflating. For inflating balloons in the wall of the main tube and the flange can be made by communicating with the cylinder and open at one end channels.

The invention is illustrated by drawings and diagrams, where:

figure 1 shows the apparatus for producing digestive juices, front view;

figure 2 - the same, top view;

figure 3 - position of the device in the gut;

figure 4 - scheme of operations with the aim of obtaining the digestive juice using the proposed device, 1st version;

figure 5 - the same, the 2nd version.

The proposed device to get the digestive juices of animals in the chronic experiment is fistulous tube consisting of a body in the form of a main cylindrical or oval tubes 1 (1, 3-5), at the upper end of which has a flange 2 (Fig 1, 2). On opposite sides of this flange is equipped with two elastic cylinder 3 (1-5), equipped with an additional flexible plastic pipes 4 for blowing up. In principle, instead of the plastic tubes 4 in the wall and the flange of the main tube 1 can be made by communicating with the cylinders 3 and open at one end channels (not shown), through which you can make the inflation of balloons.

In a known manner fistulous tube is ustanavlivaut in the duodenum 5 (figure 3). In the wall of this ulcer opposite the mouth 6 pancreatic (or common bile) duct cut a hole, through which the bowel lumen insert the upper end fistulas tube with flange 2. Mounted on the ends of the flange cylinders have along the intestine. Fistulous tube is fixed in the gut with the blend around her sero-muscular purse string suture. Then one of the cylinders 3 is inflated by injecting it through an additional tube 4 of warm water or saline solution. Then gut tube above the inflated balloon reinforced by subserosal lacing nonabsorbable sutures. As a result, the intestinal wall is formed filamentous frame 7, thus preventing the formation of a gap between the walls of an inflated balloon and easy tensile intestinal wall and thus ensuring the integrity of the overlap of the lumen of the intestine. Similarly do it with another cylinder and the adjacent intestinal segment. After the formation of filamentous frames 7 in the intestinal wall water release from cylinders and they spadats.

In the next stage, operations are performed bypass surgery concluded between the cylinders 3 of the area of the duodenum to create a workaround for chyme to the period of complete obstruction of the intestinal lumen is inflated balloons. From skinny the intestine excised intestinal segment 8 (4), and the continuity of the intestinal tract restore by imposing interventionists "end to end" (not shown). Then deformed intestinal segment 8 connects with the duodenum 5 by superimposing two enteroanastomosis end-to-side 9 and 10 (figure 4) near the external borders of the location in the colon is inflated cylinders 3. The result is an artificial way to chyme bypassing part of the intestine, Abdurasulova hyped cylinders 3.

Figure 5 presents the second option of creating a workaround for chyme. A feature of this option is that the purpose of the bypass overlapping cylinders intestine located distal (relative to the device) intestinal loop 11 by imposing interventionists "side-to-side 12 is connected with the proximal (relative to the device) by the intestine. In this case, the chyme from the upper intestine will have the opportunity to enter in the lower divisions, through the anastomosis 12.

At the end of the operation the main pipe 1 is removed from the abdominal cavity through a separate opening in the abdominal wall. Additional plastic tube 4 is also removed from the abdominal cavity to the outside. For protection from damage by animals, these tubes can be output, for example, in the interscapular region. The surgery is completed layer-by-layer suturing perational wounds of the abdominal wall.

Subsequently, when conducting research in the cylinders 3 enter the same amount of warm water or saline solution, as during surgery, resulting in a prisoner between them intestinal segment from the mouth of pancreatic (or common bile) duct is securely isolated from the rest of the intestines. Additional tube 4 is sealed with corks or any clips. After the release of fluid from the cylinders they spadats and it becomes possible participation of pancreatic juice or bile) in the process of intestinal digestion. Outside experience elastic filamentous frames in the wall of the intestine does not affect the motor and other functions, and the presence in the gut spivshihsya elastic cylinders is not a significant obstacle to the promotion of chyme in the intestine.

Using the proposed device to get the digestive juices of animals in the chronic experiment is not necessary to excise a segment of the duodenum, which is the sensor rhythm of intestinal contractions. Because of this, the relevant studies are more physiological. Out of the experiment corresponding digestive secret enters the intestine most short, in a natural way, while in the device prototype bile or pancreatic juice before get the duodenum, must pass through a long and complex form of artificial path, resulting in the probability of congestion and infection of the digestive secretions. The device is a valve and there is no need to use that, in General, improves the reliability of operation of the device.

Sources of information

1. Ilow DS. a New method of imposing a permanent fistula pancreatic duct. In Proc.: Physiology of digestion of farm animals. M., 1935, p.143-145.

2. Kienya A.I. Intestinal-pancreatic fistula tube. Auth. mon. The USSR №235241.

Apparatus for producing digestive juices of animals in the chronic experiment, consisting of a main tube, the end of which is adapted for connection to the intestine and it has a flange, characterized in that on opposite sides of the elastic flange mounted cylinders, provided with an additional tube for inflating.


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