Method for detoxification of grain affected by microflora and toxins thereof

FIELD: agriculture.

SUBSTANCE: method involves treating grain with aqueous solution of hydrochloride of glycine decilic ether, said solution having concentration of 0.2-0.3%; performing treatment for 20 min at temperature of solution of 40 C, with grain : solution ratio making 1:2.5-3.0; washing grain with running water and after dripping of water, drying grain by blowing with heated air to basic moisture content of 14%.

EFFECT: increased efficiency in detoxification of grain owing to providing complete destroying of microorganisms and products of their activities, such as toxins, and absence of peroxide oxidation products.

1 tbl, 4 ex

 

The invention relates to the processing and storage of grain.

Known methods of detoxication of animal feed by means of their preservation, namely the introduction of propionic acid number of 70 to 80 wt.% heat and steam treatment [1].

The disadvantage of the methods is that they prevent the development of microflora on the grain, but not effective for the destruction of its toxins.

There is a method of disinfection of grain by treatment with chemicals peroxide or basic character, such as 2-10% solution of hydrogen peroxide using a catalyst - chloride divalent tin [2].

The known method is effective only for the specific toxin produced by mold fungus Fusarium. In addition, there is an accumulation in solutions unusable or poor disposal of reagents used. This adversely for the processing of grain and the environment.

There is a method of detoxification of grains infected microflora and its toxins by treatment with an aqueous solution saturated with gaseous ozone of high concentration to 0.2 mg/l for 10-40 min [3].

Ozone is the strongest oxidant that after a sufficiently long contact with the grain causes in the last oxidative processes the fat part with the formation of the primary switch is red connections. Storage of grain impossible due to unmanaged, snowballing chain of oxidation of the unstable primary peroxy compounds with the formation of secondary peroxy compounds with high toxicity.

Moreover, the increase of the content of peroxide compounds in ozone treated grain corn is happening much faster than in wheat. This may be due, first, the different fat content in corn and wheat. Secondly, different qualitative composition of fat, namely the share of unsaturated (easily oxidized) fatty acids in corn accounts for up to 85%. In this regard, in the storage of grain treated with ozone for 30 days, the content of the secondary peroxy compounds (benzidine number) 5-8 times higher compared to the initial content.

The technical result - the development of the method of detoxifying the grain, ensuring complete destruction of microorganisms and their metabolic products - toxins and the absence of peroxide oxidation.

The technical result is achieved by the fact that in the known method, including the treatment of the grain with an aqueous solution of chemical substances according to the invention, as a chemical substance used hydrochloride delovogo ester of glycine, solution concentration of 0.2-0.3%. Processing what we spend for 20 minutes at a solution temperature of 40° With, when the ratio of grain:solution 1:2,5-3,0, then the grain is washed with running water and after draining the grain is dried by blowing heated air to the initial moisture content of 14%.

Hydrochloride delovogo ester of glycine is a salt of the ester of the amino acids glycine and is a white scaly crystals with molecular weight 251,1, melting point 87-89°S, soluble in water and alcohol.

The structural formula is:

H2NCH2COOC10H21·HCl

Hydrochloride delovogo ester of glycine has a high bactericidal activity, therefore it is used for sanitizing milking equipment and milk pipes [4]. The result is the death of putrefactive (proteolytic) bacteria that cause spoilage of milk and pathogens causing food poisoning and infectious diseases.

Disinfection smooth surfaces such as tile, glass, clay tiles and the lack of toxicity of the drug, as well as the complete destruction of 1.5 hours under natural conditions can be recommended hydrochloride delovogo ester of glycine for disinfection of equipment and premises food production [5].

The method was carried out as follows. Hydrochloride delovogo ester of glycine were obtained in laboratory conditions. Stage I got hydrochloride glycine is. For this purpose heated to 100-120°With a suspension of 100 g of glycine and 30 ml of concentrated hydrochloric acid was barbotirovany within 15-20 min of hydrogen chloride until complete homogenization of the reaction mixture. After cooling the mass in the mould it was filtered, washed precipitate on the filter with acetone and dried in the air.

The output of glycine hydrochloride 99%.

Melting point 184-185°C.

In the second stage received hydrochloride delovogo ester of glycine.

For this purpose, the mixture of the hydrochloride of glycine and delovogo alcohol in the ratio 1:3 with constant stirring, they were heated to 110-120°, barbotine through the reaction mass, a current of dry nitrogen.

Kept at this temperature the mixture for 8 hours. After 0.5 hours after the start of exposure was passed into the reaction mass of dry hydrogen chloride for 10-15 min with a speed of 20-40 bubbles per minute. This operation is repeated 2-3 hours and 1 hour before the end of the process. The solution is completely homogenized in 5 hours after the start of exposure.

After the reaction, to the mixture was added 100 ml of acetone and heated the mass at 60-70°and With constant stirring for 10-15 minutes, cooled, filtered and air-dried.

The product yield of 95%. Melting point 87-89°C.

Then got an aqueous solution of the hydrochloride delovogo ester of glycine concentration of 0.2-0.4%.

Note the p 1. Wheat is not suitable for feeding infected microflora and containing toxins (aflatoxin, ochratoxin, trichothecin)were loaded into a container with a stirrer was filled with an aqueous solution of hydrochloride delovogo ester of glycine at a rate of 1:2,5-3,0. The temperature of the solution 20°C. was kept for 30 min with periodic mixing. Then the grain was unloaded on the tray with mesh bottom and washed with running water. After draining the water, the grain was dried by blowing heated air to the initial moisture content of 14%.

Example 2. Wheat is not suitable for feeding infected microflora and containing toxins (aflatoxin, ochratoxin, trichothecin and others)were loaded into a container with a stirrer was filled with an aqueous solution of hydrochloride delovogo ester of glycine temperature of 40°With a rate of 1÷2,5-3,0 kept for 20 min with periodic mixing.

Further according to the example 1.

Example 3. Corn is not suitable for feeding infected microflora and contains toxins were loaded into a container with a stirrer was filled with an aqueous solution of hydrochloride delovogo ester of glycine temperature of 20°With a rate of 1:2.5 to 3.0, and kept for 30 min with periodic mixing. Further according to the example 1.

Example 4. Corn is not suitable for feeding infected microflora and contains toxins were loaded into a container with a stirrer, for alkali aqueous solution of hydrochloride delovogo ester of glycine temperature of 40° With the rate of 1:2,5-3,0. kept for 20 min with periodic mixing. Further according to the example 1.

The results of processing of grain are presented in the table.

td align="center"> no.
Name sampleSolution concentration, %The temperature of the solution, °The ratio of grain and mortarProcessing time minThe microflora content (colonies per 1 ml of extract of grain)The content of toxins, mg/kg grainThe content of the residual solution after treatment mg/kg grain
before processingafter processingbefore processingafter processing
Corn0,1201:3,0307261688,390,57no.
Wheat0,15201:3,02072149,010,20no.
Corn0,2201:3,030723no.7,98no.
Corn0,2401:2,520756no.8,23no.no.
Wheat0,2401:3,020722no.8,44no.no.
Corn0,3201:3,030718no.8,14no.no.
401:2,525730no.to 9.32no.no.
Wheat0,4201:2,520734no.for 9.47no.no.
Corn0,4201:2,520829no.11,22no.no.

The increase in the content of the primary and secondary peroxy compounds in wheat and maize during storage for 4 months after it is processed by the proposed method is not marked.

Deviations of the growth and development of the experimental broiler chickens in the result of feeding them processed grain by the proposed method directly after treatment and after 4-month storage was not detected.

The application of the solution concentration less than 0.2% does not provide a complete loss of microflora and its toxins.

Applying a solution temperature of less than 20°increases the exposure time of the grain in solution and does not guarantee the complete destruction of toxins.

When the ratio of the grain:the solution is less than 1:2.5 to need continuous mixing of the grain in the tank, which leads to increased energy consumption.

Sources of information taken into account in the examination

1. SU # 461486, class. And 23 To 3/00, 1972.

2. SU # 511834, class. And 01 No. 21/00, 1974.

3. SU # 718072, class. And 01 No. 21/00, 1980.

4. RU # 2238001, class. And 23 With 7/02, 2004.

5. IVAN Gorlov, Karenina T.V. Scientific and practical rationale for intensification of production of competitive products livestock and complex processing. Volgograd, 2002, p.81-86.

Way of detoxifying the grains infected microflora and its toxins, including the treatment of the grain with an aqueous solution of chemical substances, characterized in that as a chemical substance used hydrochloride delovogo ester of glycine, solution concentration of 0.2-0.3%, and the treatment is carried out for 20 minutes at a solution temperature of 40°when the ratio grain:solution 1:2,5-3,0, then the grain is washed with running water and after draining the grain is dried by blowing heated air to the initial moisture content of 14%.



 

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