Preparation for regulating menstrual cycle in case of secondary normogonadotropic amenorrhea and oligomenorrhea and method for therapy
FIELD: medicine, gynecology.
SUBSTANCE: as a suggested preparation one should apply dried defibrinated or non-defibrinated blood of Siberian stags or spotted deer. The preparation suggested should be prescribed perorally per 25 mg (1 capsule) twice daily, therapy course corresponds to 25-35 d. Application of this preparation in women at normal level of gonadotropins, positive gestagen-test, with no hyperandrogenia and hyperprolactinemia at the absence of menstrual cycle of more than 3 mo provides the onset of menstruations at stable positive effect. After carrying out 3 therapeutic courses no relapses had been observed.
EFFECT: higher efficiency for regulating menstrual cycle.
3 cl, 2 ex, 3 tbl
The invention relates to medicine, specifically to the gynecologist, and for funds that contribute to the regulation of the menstrual cycle when the secondary normogonadotrophic amenorrhea and oligomenoree and execution of treatment method.
Known means for the treatment of secondary normogonadotrophic amenorrhea and oligomenorrhea: drugs ovarian hormones, gonadotropins, antiestrogens and non-hormonal means [1, 2, 3]. Known methods of treatment of secondary normogonadotrophic amenorrhea and oligomenorrhea by assigning these drugs[1, 2, 3].
The closest to the origin (the prototype) is a treatment for secondary normogonadotrophic amenorrhea and oligomenorrhea Remains, the drug of plant origin, and method of treatment of secondary normogonadotrophic amenorrhea and oligomenorrhea by assigning Remainsa 20 drops 3 times a day, 30 minutes before a meal, the rate of 6-14 weeks .
Medium plant origin Remains contains (per 100 ml): Cimicifuga D1 - 5 ml Sanguinaria D6 - 10 ml, Aristolochia D6 - 10 ml, Sepia D12 - 10 ml, Lachesis D12 - 10 ml ethanol 43% by weight. The drug is approved by the pharmacological Committee of Ministry of health of Russia (June 14, 2001), registration number - P # 013177/01-2001.
However, the use of this tool is not effective (restore menstruation in 50% of cases), an unstable result of treatment (relapse Amen is Rhea and oligomenorrhea after discontinuation of the drug in 64.3% of cases). Furthermore, it is noted some adverse effects in the form of occurrences of pain in the lumbar region (10,7%), increase of blood glucose level in patients with impaired glucose tolerance (3,6%).
The problem solved by the invention is to eliminate the disadvantages of the prototype, improve treatment, reduce side effects, frequency of relapse and expanding Arsenal of non-hormonal means used for the treatment of secondary normogonadotrophic amenorrhea and oligomenorrhea.
This object is achieved in that, according to the present invention as a means for regulation of the menstrual cycle in the treatment of secondary normogonadotrophic amenorrhea and oligomenorrhea use dried defibrinating or nedifferentsirovannoi the blood of the deer, the deer or spotted deer (female or male) [4, 5]. The tool is given orally at 25 mg (1 capsule) 1 times a day, the course of treatment is 15-25 days break between courses 3-7 days, the number of courses 3.
From the analyzed literature known biogenic property (improved mental and physical performance, activation of metabolism, accelerate tissue regeneration) products containing dried defibrinating blood and adaptogenic and nootropic property products containing dried nedifferentsirovannoi cu is V deer, the deer or Sika deer [4, 5], but does not know his new property, contributing to the regulation of the menstrual cycle when the secondary normogonadotrophic amenorrhea and oligomenorrhea. The set of distinctive features is not obvious to a person skilled in the art.
Technical solution can be successfully used in clinical gynecology as a means for regulation of the menstrual cycle in the treatment of secondary normogonadotrophic amenorrhea and oligomenorrhea.
Thus, the proposed solution meets the criteria of the invention of "novelty", "inventive step" and "capable of industrial application".
The use of dried defibrinating or nedifferentsirovannoi deer blood, deer or Sika deer for the treatment of secondary normogonadotrophic amenorrhea and oligomenorrhea became possible thanks to the conducted clinical research.
Dried defibrinated or nedifferencirovannaja the blood of the deer, the deer or axis deer is a deer blood, deer or spotted deer, separated (defibrinated blood) and undelimited (nedifferencirovannaja blood) from fibrin. Upon receipt defibrinated blood blood taken from a deer, the deer or spotted deer, separated from fibrin and dried to achieve a moisture content of 4-5% in vacuum, e is the bone with the walls, heated to 45° . To obtain nedifferentsirovannoi blood, the blood of the deer, the deer or spotted deer immediately after the capture is loaded into the drying apparatus and dried to achieve a moisture content of 4-5% in the apparatus to walls, heated to 45-50° .
First it is shown that the use of dried defibrinating or nedifferentsirovannoi deer blood, deer or spotted deer leads to the regulation of the menstrual cycle and pregnancy.
A survey of a group of women. The total number of surveyed amounted to 23 people: women aged 17 to 35 years, suffering from secondary amenorrhea and oligomenorrhea with normal levels of gonadotropins, positive gestagen test without giperandrogenii and hyperprolactinemia. The survey was conducted according to the who Protocol. Disease duration was 6.2±2.8 months.
The study was conducted according to the following parameters:
1. History (General, obstetric, medical history).
2. General physical examination (including examination of mammary glands and counting hirsute number), gynecological examination (bimanual examination, inspection mirrors).
3. Determination of the level of follicle-stimulating hormone of the pituitary gland, luteinizing hormone of the pituitary and prolactin in serum CROs and; according to the testimony of the level of thyroid-stimulating hormone of the pituitary, thyroid hormone, testosterone.
4. Ultrasound of the pelvic organs (length, width and anteroposterior size of the uterus, endometrial thickness, ovarian volume, the size of the follicles).
5. Measurement of basal body temperature during treatment.
All women received for regulation of the menstrual cycle remedy containing dried defibrinating or nedifferentsirovannoi the blood of the deer, the deer or spotted deer. The tool is administered orally daily 1 time per day in a dosage of 25 mg (1 capsule), the rate of 15-25 days, 3 courses of treatment with an interval of 3-7 days.
The study made it possible to identify a sufficiently pronounced effects dried defibrinating or nedifferentsirovannoi deer blood, deer or Sika deer: the onset of menstruation (73,9%), pregnancy (13%), which allowed us to use this tool as a means for regulation of the menstrual cycle in the treatment of secondary normogonadotrophic amenorrhea and oligomenorrhea. Table 1 provides a comparative analysis of treatment of secondary normogonadotrophic amenorrhea and oligomenorrhea herbal drug Remains and product containing dried defibrinating or nedifferentsirovannoi the blood of the deer, the deer or spotted deer. Table 2 shows the frequency of us is upline pregnancy in the treatment of secondary normogonadotrophic amenorrhea and oligomenorrhea herbal drug Remains and means containing dried defibrinating or nedifferentsirovannoi the blood of the deer, the deer or spotted deer.
The method is as follows. After diagnosis secondary normogonadotrophic amenorrhea and oligomenorrhea according to clinical and paraclinical criteria the patient for regulation of the menstrual cycle prescribe a product containing dried defibrinating or nedifferentsirovannoi the blood of the deer, the deer or spotted deer orally 25 mg (1 capsule) once a day, within 15-25 days. The treatment was repeated 3 times, with an exchange between 3-7 days. Conditions for the start of treatment: absence of menstruation for more than 3 months; normal levels of follicle-stimulating and luteinizing hormones of the pituitary gland, prolactin, as well as positive gestagen test.
Justification mode. The mode of drug was selected on the basis of earlier experimental and clinical studies.
The purpose of the tool 25 mg once a day for 15-25 days is sufficient to obtain the desired positive effect. To achieve long-lasting positive effect allows 3 course assignment means less than 3 courses causes a relapse of the disease. So, according to clinical studies in women who received only 1 course of therapy on the proposed method, che is ez 3-5 months was observed relapses, women who received 2 courses, recurrences occurred in 5-7 months. Women who received 3 courses, recurrences are not marked. The interval between courses due to the duration of menstruation.
Most of the women (56.5%) positive effect was observed after 1 year of receiving the funds. However, as has been shown in clinical studies to achieve a lasting positive effect tool was administered on 3 courses of treatment. Table 3 shows the comparison of efficiency of different number of courses of treatment secondary normogonadotrophic amenorrhea and oligomenorrhea with the use of products containing dried defibrinating or nedifferentsirovannoi the blood of the deer, the deer or spotted deer.
The application of the proposed funds for a new purpose in gynecology, as a means for the treatment of secondary normogonadotrophic amenorrhea and oligomenorrhea became possible thanks revealed his new property.
Specific examples confirming the effect of the drug on the regulation of the menstrual cycle and complete method.
1. Patient B., aged 27. When the treatment complained of the absence of menstruation for 2 years after the birth of a child.
History: Menarche at age 13, menstruation was established after 3-4 days, after 28 days, moderate, painless. Pregnancy - 1, childbirth is 1 in 20 years (asked them whose likeness the section).
Objective status. The condition is satisfactory. The shape correct. The skin intact. Subcutaneous fat is developed moderately, evenly distributed. Mammary glands: symmetric, painless on palpation, discharge from the nipple no, seals are not identified. Hirsute 5 points (on a scale of Ferriman-Gallwey).
Status genitalis. External genitals are developed correctly. Hair growth on the female type. The uterus is normal in size, mobile, painless. The appendages are not enlarged, bezboleznennyy. The cervix is not visually changed. Vaginal mucosa is pink. Beli mucous. Symptom pupil "+". The elongation of the cervical mucus 2 see
Laboratory studies of hormones in the serum. Follicle-stimulating hormone and 6.6 IU/l, LH - 1,44 IU/l, prolactin - 132 mlu/ml, tireo genotype hormone - 0,877 mlu/ml, T4- 61,1 nmol/l, T3- 0.82 nmol/L. Gestagen test positive.
Diagnosis: Secondary normogonadotropic amenorrhea.
According to the proposed method the patient for regulation of the menstrual cycle was appointed means containing dried defibrinating blood: oral, daily, 1 times a day, the dosage of 25 mg (1 capsule), course - 20 days, a break between courses 7 days. Conducted two courses of treatment. After the second course of the drug at which aziende came menstruation. Appointed third year, 6 days after the second course. Conducted a follow-up examination after 3, 6 and 12 months. The woman remains a regular menstrual cycle.
2. Patient K., 19 years. Complaints about the lack of menstruation for 4 months.
History: Menarche at the age of 13, during the years of menstruation came regularly after 30 days, 5 days, moderate, painless. Then there were delays menstruation 3-8 months.
Status genitalis. External genitals are developed correctly. Hair growth on the female type. The uterus is not enlarged, painless, mobility stored. The appendages are not palpated. The cervix is not visually changed. Vaginal mucosa is pink. Beli mucous. Symptom pupil "+". The elongation of the cervical mucus 3 see
Objective status: satisfactory Condition. The shape correct. Skin clean. Subcutaneous fat is poorly developed, evenly distributed. The breast is developed symmetrically, painless on palpation, discharge from the nipple there. Hirsute number 4 points (on a scale of Ferriman-Gallwey).
Laboratory studies of hormones in the serum. Follicle stimulating hormone is 6.5 IU/l, LH - 3,93 IU/l, prolactin - 403 mlu/ml. Gestagen test positive.
Diagnosis: Oligomenorrhea with normal levels of gonadotropins.
According to offer and is by the way the patient to regulate the menstrual cycle was appointed means, containing dried nedifferentsirovannoi blood oral daily 1 time per day in a dosage of 25 mg (1 capsule), the rate - 25 days. After the first course of the drug, the patient came menstruation. Assigned to the second and third courses with breaks at 5 and 7 days, respectively. Within six months of the menstrual cycle was regular, then pregnancy.
Thus, the obtained results allow to conclude that dried defibrinated or nedifferencirovannaja the blood of the deer, the deer or spotted deer affects women early reproductive age with secondary normogonadotrophic amenorrhea and oligomenorrhea on the regulation of the menstrual cycle and increases the effectiveness of treatment, reduce side effects, the frequency of relapse and expands the Arsenal of non-hormonal means used to regulate the menstrual cycle when treatment secondary normogonadotrophic amenorrhea and oligomenorrhea.
|Treatment||The onset of menstruation|
|The absolute number||%|
|Dried defibrinated or nedefinirana blood (=23)||17||73,9*|
|* Note: there is a statistically significant difference relative frequencies of recovery of the menstrual cycle in women treated product containing dried defibrinating or nedifferentsirovannoi blood when compared with a group of women, who as a treatment drug Remains. The achieved significance level of p=0,0434 (critical p=0,05). To compare the relative frequencies were used Z-statistics normal distribution with the amendment of Iatse.|
|Treatment||The onset of menstruation|
|The absolute number||%|
|Dried defibrinated or nedefinirana blood (n=23)||3||13,0|
|The number of treatment courses||The onset of menstruation|
|The absolute number||%|
1. Regulation of the generative functions. The who Symposium, 1976 / edited Persianinova PS the manuylova playing I.A. Russian edition. - Copenhagen: Scriptor. - 1978. - 140 C.
2. Runnebaum Century, Rabe T. Gynecological Endocrinology. - Springer-Verlag Berlin Heidelberg. - 1997. - V.1. - P.223, 209, 225.
3. Yevtushenko I.D., Bolotov VP, Gaifulina IF, Kutsenko WAS the Effectiveness of the application of the new non-hormonal drug Remains in the treatment of secondary amenorrhea // New non-synthetic drugs. - Moscow. - 2000. - p.17-23.
4. Biogenic drug, food additive based on it and the way they are received. RF patent №2163813. - Invention. Utility models (the official Bulletin of the Russian Agency for patent and trademarks). - Moscow. - FIPS. No. 7. - t.ii. - 2001. - S-171.
5. The agent with neuroprotective and adaptogenic action, and how to obtain it. RF patent №2130314. - Inventions (official Bulletin of the Russian Agency for patent and trademark is m). - Moscow. - FIPS. No. 14. - vol. - 1999. - P.472.
1. The use of dried defibrinating or nedifferentsirovannoi deer blood, deer or Sika deer as a means of regulating the menstrual cycle in the treatment of secondary normogonadotrophic amenorrhea and oligomenorrhea.
2. A method of treating secondary normogonadotrophic amenorrhea and oligomenorrhea involves oral administration of pharmacotherapy, characterized in that designate dried defibrinating or nedifferentsirovannoi the blood of the deer, the deer or spotted deer at a dose of 25 mg for 25-35 days.
3. The method according to claim 2, characterized in that the drug is presented in the form of capsules of 25 mg, which are prescribed 2 times a day.
FIELD: organic chemistry of heterocyclic compounds, pharmacy.
SUBSTANCE: invention relates to new bicyclic heteroaromatic compounds of the general formula (I): wherein R1 represents phenyl optionally substituted with NHR5 or OR5; R2 represents (C1-C4)-alkyl or phenyl; R5 represents phenylcarbonyl, (C4-C6)-heterocycloalkylcarbonyl, (C2-C8)-alkenylsulfonyl and others; Y represents nitrogen atom (N); Z represents -NH2 or -OH. A represents sulfur atom (S) or a bond; B represents -N(H) or oxygen atom (O); X1-X2 represent C=C, -NH-C(O), C=N and others; Proposed compounds show agonistic activity with respect to LH receptor and can be used in medicine.
EFFECT: valuable medicinal properties of compounds.
10 cl, 34 ex
SUBSTANCE: the present innovation deals with composition for fertilization in vitro and the system for its delivering (device). The composition suggested contains steroid at the quantity of below 5% (weight/weight), that is: 4.4-dimethyl-5α-cholesta-8.14.24-trien-3β-ol, hemisuccinate of 4.4-dimethyl-5α-cholest-8.14.24-trien-3β-ol; 5α-cholest-8.14-dien-3β-ol; hemisuccinate of 5α-cholest-8.14-dien-3β-ol; (20S)-cholest-5-en-3, 20-diol; N-(methionine)amide of 3β-hydroxy-4.4-dimethyl-5α-chol-8.14-dien-24-oic acid or cholest-5-en-16β-ol, and, also, additive (water-soluble protein or phosphoglyceride). Delivering system has got either one foramen or one cavity that contains the composition mentioned as a solid product or solution. The composition of sterols contains no constituents negatively affecting oocytes and could be dissolved in aqueous medium without physical impact (that is, heating, mixing or ultrasound treatment).
EFFECT: higher efficiency of fertilization in vitro.
8 cl, 5 ex, 3 tbl
FIELD: medicine, urology.
SUBSTANCE: at the background of curative starvation a patient should be prescribed for peroral intake of 100 ml tincture or 1 teaspoon tincture out of bees twice daily in the morning and in the evening, and, also, 200 ml hempseed decoction based upon milk twice daily in the morning and in the evening at the course of 5 d. On the 3d, 5th, 7th d of starvation one should perform hepatic and biliary ductal purification at 7 p.m. at the intake of 250 ml natural apple juice at 4 p.m., since the 1st to the 7th d of starvation one should daily apply enemas: in the morning - purifying enema with juice of 1 lemon, in the daytime - curative enema with Artemisia decoction. One should daily perform rectal prostatic massage followed by applying microenemas with 30 ml composition containing the tincture of Sophora, oil of wheat germs, decoction of medicinal plant of antiphlogistic action taken at weight ratio of 1:0.5:2, correspondingly, at the course of 10 d long. Duration of curative starvation lasts for 7 d, not less. The method provides shortened terms of therapy due to simultaneous normalization of body metabolic processes, purification of organs against bacteria and parasites.
EFFECT: decreased body allergization.
6 cl, 2 ex
FIELD: veterinary science.
SUBSTANCE: on should apply 1-(ethoxy)sylatrane as a stimulating agent for reproductive capacity in female furred animals and viability of their offspring. The innovation enables to improve reproductive function in females and viability of whelps.
EFFECT: higher efficiency.
3 ex, 3 tbl
FIELD: pharmaceutical industry.
SUBSTANCE: invention provides therapeutical formulation for improving fertility and treating sterility caused by polycystic ovary syndrome, which composition is characterized by that formulation thereof involves use of dipeptidylpeptidase IV inhibitor. Treatment of polycystic ovary syndrome-caused sterility is high successful with effective active ingredient concentration equal to 1 μM. When dipeptidylpeptidase IV inhibitor is administered, luteinizing hormone and testosterone levels in blood are normalized. Claimed compounds are low toxic for warm-blooded animals and humans.
EFFECT: widened choice of efficient antisterility drugs.
7 cl, 3 ex
FIELD: medicine, in particular provision and treatment of donor blood.
SUBSTANCE: claimed method includes whole blood centrifugation, component separation, filtration of erythrocyte mass and plasma, and storage under sterile conditions, wherein leuko-platelet layer id separated. Firstly plasma is filtered, part of plasma is kept in filter, and erythrocyte mass is filtered through plasma-containing filter. Residual plasma is removed before erythrocytes begin to pass though filter, followed by blending with filtered plasma.
EFFECT: economy method for separation and storage of leuko-platelet layer; decreased losses of blood components.
1 ex, 1 tbl
FIELD: medicine, gynecology.
SUBSTANCE: invention relates to an agent used in treatment of vasomotor and emotional-vegetative symptoms of climacteric syndrome and the corresponding method for treatment. Method involves using dried defibrinated or non-defibrinated blood of Siberian stag, Manchurian wapity or sika deer (females or males). Agent is prescribed by oral route administration in the dose 25 mg (1 capsule), 2 times per a day for 25-35 days. Dried defibrinated or non-defibrinated blood of Siberian stag, Manchurian wapiti or sika deer enhances the level of estradiol and declines the level of follicle-stimulating hypophysis hormone in serum blood that provides the clinical effectiveness of treatment.
EFFECT: valuable medicinal properties of agent.
3 cl, 2 ex
FIELD: technology for preparing biologically active compounds from animal raw.
SUBSTANCE: invention relates to industrial method for isolation of iron-containing protein - hemoglobin from blood of agricultural animals. Raw for preparing hemoglobin represents erythrocyte mass from stabilized whole blood, blood cells or nonstabilized coagulated whole blood that is finely milled preliminary to particles size 1-5 mm. After preliminary destruction of erythrocytes the raw is diluted successively with distilled water for extraction of hemoglobin and alleviating separation of erythrocyte membranes, fibrin and stroma. Hemoglobin solution is prepared by using a biphase system for separation of erythrocyte mass hemolyzate obtained by its successive dilution with distilled water with addition of 1-3% of sodium chloride in the ratio = (0.1-2):1 of raw volume and at maintaining pH value of solution in the range 5.5-8 at temperature 4-10C. After successive carrying out the first, second and third extraction and further purification by using membrane macro-, micro- and ultrafiltration and ion-exchange chromatography, stabilization and drying by using low-temperature spraying or sublimating drying the end products are prepared with the enhanced yield of hemoglobin up to 98% comprising from 60 to 99% of hemoglobin with the content of iron from 0.16 to 0.3% in reduced form. Hemoglobin of different purity degree can be used as a concentrated natural source of biologically available iron in manufacturing functional foodstuffs, biologically active supplements, fodders, veterinary and medicinal preparations used in prophylaxis and treatment of iron-deficient states in humans and animals. Proposed group of inventions promotes to simplifying and reducing cost of the process in industrial preparing blood hemoglobin from agricultural animals with simultaneous improvement of quality and its enhanced yield.
EFFECT: improved processing and preparing method.
25 cl, 2 dwg, 9 tbl, 5 ex
SUBSTANCE: method involves holding in solvent so that fibrin is separated by pressing blood clot through net, collected, washed from residual hemoglobin, dissolved in 4-6% ammonia solution on the basis of 1 g of raw fibrin per 5-6 ml of solution and then hold in solvent under continuous shaking at 20-25°C for 45-60 min. Fibrin paste is produced using solvent dissolvable on the surface. When covering wound or ulcer surface with the paste, ammonia evaporates leaving only fibrin on the surface.
EFFECT: high quality of product.
FIELD: medicine, oncology.
SUBSTANCE: the present innovation deals with complex treatment of cancer, prevention of relapses and cancer metastases, in particular, locally metastasing cancer of large intestine and mammary gland. The innovation deals with surgical therapy and chemotherapy. Moreover, in preoperational period one should sample 200 ml patient's blood, due to centrifuging one should isolate autoleukothrombomass at the quantity of 20 ml to be placed into the first vial with chemopreparations. The rest blood elements should be put into the second vial together with plasma and chemopreparations. Separately, one should incubate the vials for 40 min at 37° C, then comes an operation in the course of which one should intravenously by drops introduce incubated blood with chemopreparations from the second vial. On removing the tumor, one should infiltrate the channel of mammary cancer or the fiber of retroperitoneal space at the side of colonic tumor localization with incubated autoleukothrombomass with chemopreparations from the first vial. The innovation enables to decrease the risk of developing local relapses, remote tumor metastases and postoperational complications.
EFFECT: decreased toxicity of therapy.
SUBSTANCE: method involves making surgical intervention after Widman with patient blood taken in the amount of 10-20 ml before placing flap. The blood is centrifuged during 11-13 min at 2400-2800 rpm and divided into 3 fractions like erythrocytes, plasma of high blood platelets content and plasma of low blood platelets content as a result of the treatment. The plasma of high blood platelets content is taken from laboratory glass and mixed with beta-tribasic calcium phosphate powder in proportion of 1:3. Then, the produced mixture is placed into defect zone and the flaps are fixed with sutures.
EFFECT: no additional surgical intervention needed; excluded allergic responses.
FIELD: food-grade preparation with increased content of haem iron complexes.
SUBSTANCE: claimed method includes treatment of hog or bovine blood or derivative thereof, such as blood coagulum, wherein the firs animal blood fraction is subjected to soft enzymatic hydrolysis to produce the first hydrolyzate having hydrolysis ratio of at most 5 %. Then the first hydrolyzate is filtered by ultrafiltration to produce hydrolyzate solution enriched with haem iron-peptide complex. The second animal blood fraction is simultaneously subjected to complete enzymatic hydrolysis to produce the second hydrolyzate having hydrolysis ratio of at lest 15 %. Prepared second hydrolyzate is centrifuged to produce concentrated haem deposition. Obtained deposition is dissolved in sodium hydroxide to produce joint haem solution wherein haem is bound to small peptides. Hydrolysate solution enriched with haem iron-peptide complex is blended with joint haem solution. Mixture is centrifuged and obtained solution is enriched with haem iron-peptide complexes by ultrafiltration to remove small peptides released in blending step. Food-grade preparation obtained by abovementioned method also is disclosed. Said preparation is in liquid form of in form of haem peptide powder with iron/peptides weight ratio more than 2 %, wherein more than 80 % of said iron is in form of peptide complex. In another embodiment food-grade preparation may be in form of gelatin capsules of 0?5 ml volume.
EFFECT: new food-grade preparation with increased content of haem iron complexes.
FIELD: food and medicinal industry.
SUBSTANCE: invention relates to a method for preparing biologically active product of velvet antlers reindeer breeding. Velvet antlers is milled to particles size 0.5-1 cm3, mixed with deer blood and frozen to -50°C. Freezing and drying is carried out by effect of mechanical low-frequency oscillation with frequency 6- Hz, not less, and with amplitude from 5 to 12 mm by vibration of heat-exchange surface, for example, sublimator device plates wherein on this surface the raw is frozen and dried. Drying is carried out in regimen of temperatures from -50°C to +50°C and in the pressure range from 0.3 to 2 mm of mercury column. Method allows accelerating the drying process of raw taken in any ratios raw : blood, to enhance biological activity of ready product due to acceleration of the drying process, low temperature of drying, and to improve the technical grade quality and expand raw source in preparing biologically active supplements.
EFFECT: improved preparing method.
3 tbl, 1 ex
FIELD: medicine, hematology.
SUBSTANCE: the present innovation deals with utilizing the wastes (clots) of human and animal blood to obtain curative preparation out of them. The method deals with isolating fibrin out of a blood clot followed by dissolving and applying it onto the surface up to fibrinous layer thickness being about 5-7 mm with subsequent drying. Moreover, isolated fibrin should be dissolved at 21-25°C in 8-10%-ammonia solution at 1 g crude fibrin/3-5 ml solution for 3-5 d, and it should be dried at an even surface pre-treated with 50%-glycerol solution at 30-40°C. The innovation enables to obtain a film out of blood clot's fibrin without applying salt in order to obtain the final product containing fibrin only.
EFFECT: higher efficiency.
FIELD: medicine, hematology.
SUBSTANCE: the present innovation deals with developing new ways for obtaining fibrin preparations. Method for obtaining disinfecting powder of fibrin out of human and animal blood clots deals with drying and mechanical reducing, moreover, raw mass of fibrin should be precept for 3-5 d in 0.05%-chlorohexidine bigluconate solution at 20-25°C. The innovation enables to obtain sterile powder of fibrin protected against the action of microorganisms, out of blood clots.
EFFECT: higher efficiency.
FIELD: medicine, surgery.
SUBSTANCE: at the background of basic therapy in complex therapy of acute pancreatitis one should introduce ceruloplasmin to be applied at the dosage of 600-1000 mg/d for 5 d. If necessary, the course of ceruloplasmin introduction should be repeated. This method provides pancreatic tissues viability in case of pancreonecrosis by increasing efficiency of correction the endogenous intoxication and decreasing the number of complications in the course of therapy conducted.
EFFECT: higher efficiency of pharmacological correction.