Extract for treatment of periodontum diseases

FIELD: medicine.

SUBSTANCE: invention relates to brown algae-based medicative and prophylaxis preparation for treatment of periodontum inflammations. Claimed composition contains extract obtained from Ascophylum nodosum and Fucus vesiculous algae and water in the next mass ratio (gram): extract 1: water 100.

EFFECT: preparation stimulating lactobacterium production and not causing mouth cavity dysbacteriosis.

23 tbl

 

The invention relates to medicine, in particular to the dentist, and can be used for the treatment and prevention of inflammatory periodontal diseases. For the treatment of inflammatory periodontal diseases apply various antiseptic solutions and herbal remedies.

The disadvantage of the use of antiseptics is the development of dysbiosis after application, however, a drawback when using herbal remedies for the treatment of inflammatory periodontal disease is the low efficiency.

The aim of the invention is to eliminate these disadvantages and the establishment of stable remission. Used a 1% solution of the extract "Fucus".

The results of biomedical testing products from fucoidan.

The objects of study were the drugs of algae Ascophyllum nodosum and Fucus vesiculosus.

The aim of the work was in medico-biological studies of biologically active algal additives: evaluation of General toxic effect, the study of allergenic and antimicrobial action, efficacy.

It is shown that the investigated drugs do not have a General toxic, immunotoxic and allergic effects have a direct antimicrobial activity against Escherichia coli and Staphylococcus aureus and a pronounced protective effect on models of secondary immunodeficiency of various origins. All the research on the drugs possess antioxidant activity, reducing the number of products of lipid peroxidation.

Evaluation of toxic properties BAD from fucoidan in the acute and subacute experiments.

Study of acute toxicity of dietary SUPPLEMENTS.

Study of acute toxicity of the drugs was performed on males is two months old outbred mice weighing 18-20 g of the Animals were on a steady diet and maintained according to sanitary rules. The introduction of the preparations was carried out according to the sanitary rules. The introduction of drugs was performed in the morning hours. Acute toxicity was studied in 2 routes of administration - oral and intraperitoneal. Before orally administered animals were odarivali for 40 minutes in an empty cell. Preparations were examined in the range of 4-5 doses sufficient to calculate srednesemennyh doses. Each group contained 6 animals. Calculating LD50was conducted on the Cerberus. In the study Krupki drugs tested supernatant liquid, which was prepared as follows: pulverized substance was soaked in water (100 g/l), insisted for days at 37°and then filtered (Ministry of health guidelines of the Russian Federation from 20.04.98 year).

The total duration of observation of experimental animals was 14 days, and on the first day of the animals were monitored. Regularly recorded total is TATUS mice, peculiarities of their behavior, the intensity and nature of physical activity, response to tactile, pain, sound stimuli, the condition of the fur, feed and water intake, and other indicators of toxic effects of drugs.

The research produced srednetemperaturnyi doses for drugs (table 1). The obtained results allow us to classify the studied drugs to low-toxic.

The study of subacute toxicity of dietary SUPPLEMENTS.

The study was performed on Guinea pigs weighing 280-300 g, males. Animals were divided into following groups: the first group consisted of animals on a daily basis during the month was administered orally using a metal probe diluted 1/4 water concentrate Ascophyllum nodosum in a volume of 1.6 ml; the second group consisted of animals on a daily basis during the month was administered diluted 1/4 water concentrate Fucus vesiculosus in the volume of 1.6 ml; the third group consisted of animals on a daily basis during the month was introduced extract of grain Ascophyllum nodosum in a volume of 1.6 ml; the fourth group was made up of animals on a daily basis during the month was introduced extract of grain Fucus vesiculosus in the volume of 1.6 ml; the fifth group was the control animals, which in the same volume was injected water.

During the experiment the animals were evaluated General condition, behavior, SOS is the right hair and mucous membranes, feed and water intake. Animals were regularly weighed. The functional state of organs and systems was evaluated by determining marketnig indicators - activity of aminotransferases and Alp, urea, creatinine, peripheral blood indices.

For the definition of these indicators of blood from Guinea pigs were taken from the cavity of the heart in the amount of 4.0 to 5.0 ml.

The counting of blood cells produced by the Hematology analyzer AVG Mikros (France).

Biochemical parameters were determined on the biochemical machine Technique "Axon" using the following methods and kits:

- urea - urease, enzimaticeski, sets firm "Hyman";

- creatinine - modified Jaffe method without deproteinization, reagents firm Technikon";

aminotransferase - optimized enzimaticeski, kinetic, chemical reagents company "Vital diagnostics", St. Petersburg;

alkaline phosphatase - kinetic, reagents firm "Biosystems".

After subacute experiment conducted euthanasia of animals by an overdose of diethyl ether for pathological studies of internal organs and tissues.

Autopsy of the animals was performed immediately after their death on a full postmortem scheme that excluded possible autolysis of tissues, allowing additional is sustained fashion studies. Each animal was full minutes of opening with characteristic pathological signs in all organs and body systems.

Morphometric evaluation of body weight and organs of the animals was performed using electronic scales "Sartorius (Germany) with the subsequent calculation of the coefficients of the masses and their standard deviations.

For pathological studies samples of fresh tissue was subjected to cryostation obtaining sections of 5-7 µm or fixing them in 10% neutralized formalin solution for getting in subsequent paraffin sections with a thickness of 2-5 μm. Dehydration, degreasing and wiring tissues with their seal waxes with different melting temperature (37-57° (C) conducted in microprocessor autoclave "Sakura" (Japan) on a predetermined program; fill tissue - paraffin wax and the formation of tissue blocks - manual way. After deparaffinize slices were stained with hematoxylineosin and other azo dyes, concluded under the cover glass and prepared to visualize permanent specimens.

Microscopy was performed on a microscope "Luman", St. Petersburg.

Obtained in each observation of complex pathological data from receiving BAD Guinea pigs were compared with the data control the different animals.

Statistical processing of obtained results was performed by the Student using the computer IBM PC/at 486.

It is shown that the introduction of drugs within 30 days revealed no significant changes in the behavior and appearance of animals. They had a smooth coat, willingly eaten food, kept normal motor activity.

The body weight of the Guinea pigs in all experimental groups who received investigational drugs within subacute experience did not differ from the control (table 1).

Data from clinical blood analysis (table 2) showed no statistically significant differences between groups of animals treated with the BAD, and the control.

The levels of urea and creatinine in the serum of animals treated BAD, did not differ from the corresponding figures in the control (table 3).

To identify the possible damaging effects of BAD on the liver investigated the activity of aspartate - aminotransferase and Alp serum.

Changes in the activity of these enzymes was within the limits of physiological norm for this type of laboratory animals. Transaminases and Alp in experimental and control animals did not differ significantly (table 4).

Pathomorphological study of Guinea pigs exposed to BAD

1) Macroscopic study

Skin and visible mucous as the control and experimental animals clean. Subcutaneous fat moderately developed. The thoracic and abdominal cavities are positioned correctly. Cavity free from liquid and adhesions.

Liver dense with a smooth surface, the cut reddish-brown color. In some animals there is a plethora of vessels of the liver.

Most animals in the respiratory Department of the lungs found no signs of edema and inflammatory response.

Light air, gray-pink color.

Heart rounded, well reduced. The leaf valves thin, translucent. Under normal epicardial figure the location of the coronary vessels.

Macroscopically no marked pathological changes in the mucous of the stomach and small intestine.

Kidney in the context of moderately cravenplan with a clear division into layers. The capsule is removed easily, beneath the smooth surface.

Spleen with a smooth capsule, in the context of gray-red in color, changes in the structure of the spleen in comparison with the control was not detected.

The coefficients of the weight of internal organs are presented in table 5, which shows that these indicators in all experimental groups did not differ from the control group, indicating no toxic impact of the drugs on organs of laboratory animals.

2) Microscopic examination.

A control group.

In liver slices tightly adjacent to each other, surrounded by a triad vessels. Hepatocytes, oval in shape with a clear structure located radially along cravenplan sinusoidal capillaries. The walls of the Central veins thin, the lumen contains small clusters of blood cells.

In the cortical layer of the renal calf surrounded by loops efferent tubules of the nephrons. Capsule glomerular thin, capillary loops surrounded by a thin layer mesangial cells. The structure of the walls of the channel corresponds to the departments of the nephron.

The group treated with drugs from Ascophyllum nodosum.

Hepatoma liver consist of radially arranged beams of hepatocytes around the sinusoidal capillaries. There are no violations of histoarchitecture liver tissue. In cells of the hepatic parenchyma there are no signs of protein, vacuole-type and fatty degeneration.

In the kidneys in the cortical layer of the glomeruli with intact structure of the capillary loops, with a thin capsule. The walls of the tubules of the nephron have the structure, corresponding to its departments, gleams them available.

The study of the structure and condition of the mucous membrane of the stomach and small intestine showed no pathological changes in these departments. The mucous membrane of the stomach is lined by a single layer of cylindrical epithelium, the hell in which the cavity of the stomach was a small amount of mucus with the remnants slomannyh cells, the number which indicates a normal intensity desquamating processes. Signs of degeneration or atrophy of the epithelium are missing. The mucosa of the small intestine shows a basic cell structures and suction goblet cells. Villous Department epithelium is not modified.

The follicles of the white pulp of the spleen normal size. The ratio of white and red pulp corresponds to the norm.

According to the pathological study of the differences between groups treated with aqueous concentrate and grit, not detected.

The group treated with drugs from Fucus vesiculosus.

In the liver hepatocytes, oval shape. Cells with homogeneous cytoplasm, clear nucleus, Central vein full, the stroma is poorly developed.

The structure and condition of the mucous membrane of the stomach and small intestine did not differ from the control group of animals. There are no signs of degeneration or atrophy of the cylindrical epithelium.

In the kidney capsules of glomeruli thin-walled, capillary loops of the glomeruli have thin walls, surrounded by a moderately developed layer mesangial cells. Wall tubules lined by epithelium typical patterns, the Lumina of the tubules is free.

According to the pathological study of the differences between groups treated with aqueous concentrate and grit, not detected.

Thus the om, data macro - and microscopic studies, the relative ratios of the weight of internal organs indicate the absence of toxic effects of all tested drugs on the organism of laboratory animals.

Conclusion.

The results of the study General toxic properties of drugs in acute experiments in a single parenteral and oral administration showed no signs of toxic action on the organism of laboratory animals. Based on the definition srednesemennyh doses of drugs can be classified as low-toxic compounds. Repeated oral administration of the dietary SUPPLEMENTS had no effect on the General condition, body weight, animal behavior, and did not influence on the functional activity of organs and systems.

Clinical, biochemical and pathological studies showed no toxic effects of the tested drugs on the organism of laboratory animals during repeated oral administration.

The study of allergic effects BAD.

Assessment of allergenic BAD actions were conducted on female Guinea pigs weighing 250-300 g in the reaction system with active anaphylaxis. BAA has introduced enterline tenfold dose of 1.6 ml when diluted solution of 1/4. 21 days after injection was performed allowing injection: one group of animals (20 pigs) enterline dose is vakratsas sensitizing, and the other group intracardiac dose equal sensitizing. Previously in intact animals was tested resolving dose that did not cause toxic reactions in animals. The intensity of the anaphylactic reactions were evaluated on a 4-point scale according to the method Weigle with calculation anaphylactic index /26/.

For experiment 7, 14, 21 days produced blood sampling for the detection of sensitization animal reactions degranulation of mast cells (RDTC). The reaction was carried out with the blood sera of Guinea pigs treated with the drug, as described above. After receiving the serum was immediately frozen and kept at -20°With up to the moment of experience. The fat cells of rats received method /27/. When setting reaction of the pre-selected dose of the drug, not to exceed the non-specific destruction of cells. The reaction was carried out with whole sera. All the ingredients used in a volume of 300 μl. Before testing, set the following controls: 1) a suspension of fat cells + thinning; 2) a suspension of fat cells + normal serum from Guinea pigs, obtained prior to immunization drug; 3) suspension of fat cells + test allergen. Cells used in the experiment, if each of the controls degranulation did not exceed 5%.

The results of the study allergenic activity by si the dark active anaphylaxis was not detected in the investigated drugs sensitizing effect. Values anaphylactic index did not exceed 0.5, which distinguishes this group of products from many well-known drugs that are strong allergens. No sensitizing effects are also shown in the reaction indirect destruction of fat cells (table 10).

As can be seen from the data presented, the introduction of drugs did not cause the production of immunoglobulin E, is responsible for the manifestation of allergic reactions of immediate type.

1.3.2.3. The study immunotoxic and immunomodulatory properties of dietary SUPPLEMENTS.

The study immunotoxic properties.

Study of the effect of additives on mass and cellularity of the organs of the immune system.

Determination of the effect of dietary SUPPLEMENTS on the weight and cellularity of the organs of the immune system were performed on mice CBA. The drugs were injected daily for 15 days oral dose of 1/2 MTD, component for water concentrate Ascophyllum nodosum 7810 mg/kg for grain and 1000 mg/kg for aqueous concentrate Fucus vesiculosus - 3500 mg/kg for grain and 1000 mg/kg Studies were performed 3 days after completion of treatment.

Control animals were injected water.

Upon completion of the experiment, animals were scored and allocated thymus, spleen and lymph nodes. The cells of the organs were isolated and identified their number of conventional methods.

As shown in table d is the R, all the drugs had no toxic effects on the Central and peripheral organs of the immune system. Canceled a slight increase in weight and cellularity of the spleen compared with control groups of animals treated with the drugs Krupki.

Determination of hemagglutinin titer in the serum.

Evaluation of the effect of drugs on humoral immune response was performed to determine the level of hemagglutinin to various antigens (sheep red blood cells and ovalbumin) and determining the amount of antibodies of the IgM class.

To study the effect of the ASS on the humoral immune response to sheep erythrocytes and ovalbumin used the reaction hemaglutination with blood sera from mice be that within 15 days injected drugs in the dose of 0.5 FM. As the antigens used sheep red blood cells (at a dose of 108cells/mouse) and ovalbumin (2 mg protein/kg), which was introduced on 15 day intravenously. Selection of blood produced weekly for 4 weeks. Control animals were injected with the appropriate amount of saline solution.

For the determination of IgG class antibodies to particulate antigen (EB) used the reaction of haemagglutination. For the detection of antibodies to soluble antigen to ovalbumin used enzyme-linked immunosorbent assay on polystyrene tablets "Dynatec" p is yavlenie adsorbed antibody conjugate artemisinin antibodies with peroxidase.

The research results are summarized in tabl. Showed a significant increase in the synthesis of hemagglutinin with the introduction of an aqueous concentrate of Fucus vesiculosus at 14-21 days. Administration of an aqueous concentrate Ascophyllum nodosum caused a weak stimulation of the synthesis of antibodies at 21 days compared to control.

Study of the level of antibodies to soluble antigen with the drug showed no BAD influence on humoral immune response.

Determination of the influence of additives on the synthesis of antibodies of the IgM class.

Determination of the influence of additives on the number of antibodies of class IgM was performed in the reaction Erie Jordan /28/, counting the number of antibody - forming cells (AFC) to EB in the spleen of mice CBA. The scheme is the introduction of drugs listed in the previous section, except that the definition of ASC held on the 5th day. Control animals were injected with the appropriate amount of saline. The results of the experiment are given in table 13. Found that repeated administration of an aqueous concentrate Fucus vesiculosus extract from grain Fucus vesiculosus slightly increases the number of the KLA, however, significant differences were not found. Other drugs do not change the number of the KLA, secreting antibodies of the IgM class.

To estimate the effects of SUPPLEMENTS on cell-mediated immunity.

The effect of drugs on the delayed-type hypersensitivity to EB.

The ability of the BA is to modulate the delayed-type hypersensitivity (GST) to T-dependent antigen was studied in mice CBA, immunized intravenously EB dose of 10 cells/mouse. The drugs were administered orally at a dose of 0.5 MTD for 15 days. After 5 days was determined by the level of sensitization of animals entering the resolving dose of EB (10 cells/mouse) in 50 μl of saline into the pad of one of the hind paws of mice. The other leg served as the control, was administered an equal volume of saline. Control animals received within 15 days injected with a saline solution. Local inflammatory response was taken into account in 24 hours after the introduction of the resolving dose of antigen, determining the difference of the masses of experienced control paws and then computing the index of reaction /29/.

Together with immunomodulating ability of the preparations was assessed by comparing the individual index scores reactions of animals from the control and experimental groups.

As is seen in table 14 data for all the studied drugs was not identified inhibitory action on the functional activity of T-lymphocytes.

The BAD influence on the functional activity of T-lymphocytes.

The assessment of the possible actions of the BAD products T-lymphocytes factor that inhibits leukocyte migration /30/, were performed on mice CBA, which was injected drugs orally at a dose of 1/4 MTD for 15 days, then once intraperitoneally injected PAF containing 1250 µg of mycobacteria in 1 ml After 6 days after injection of 0.4 ml of PA is from the mice, the blood was collected, using as anticoagulant heparin. The reaction was carried out in 5 channel capillaries, adding to the blood a solution of tuberculin 20 and 40 µg/ml In control capillaries added Hanks solution containing no antigen (tuberculin). Producers of factor inhibiting leukocyte migration in this embodiment of the reaction, as well as blood lymphocytes, and migrating cells were monocytes and neutrophils. Records of the results of the reaction conducted, microscopia capillary after 24 hour incubation at 37°in moist chamber. Leukocyte migration was measured using the micrometer range. Next, calculate the rate of oppression: migration (pumas).

The data presented in table shows a slight stimulation of functional activity of T-lymphocytes drugs from Fucus vesiculosus. All drugs inhibited the production of this lymphocyte T-lymphocytes.

Determination of the functional activity of macrophages.

Study of the influence of the studied drugs on the functional activity of macrophages was performed according to the tests for determining the activity of the marker enzyme macrophage - 5'-nucleotidase, an enzyme of purine metabolism. Also, they studied the activity of macrophages in their ability to absorb dye neutral red. We have also studied the effect of drugs on the activity oxybiotic anti-infective with the role of phagocytosis in the test recovery narasinga of tetrazole (EAST). Research methods are presented in the respective sections.

Method for the determination of phagocytic activity of macrophages based on the determination of the optical density of the lysate of phagocytes, absorbing particles of the dye neutral red /31/. The experiments were conducted on mice be normal and immunocompromising. To create immunosuppression animals were treated with azathioprine scheme 25 mg/kg orally three times, 50 mg/kg intraperitoneally once. To obtain suspensions •macrophages animals were injected intraperitoneally medium 199 containing 20% fetal calf serum.

Study of the protective action of the drugs was performed in the following groups:

1 - intact animals;

2 - animals treated with the drugs for 15 days;

3 - animals treated with azathioprine;

4 - animals treated with azathioprine and investigational drugs.

The results of the study phagocytic activity of macrophages using neutral red with a repeated study drug showed that all the studied drugs in varying degrees contribute to the activation of phagocytosis in animals on the background of immunosuppression. The most pronounced activating effect on the adsorption capacity of the macrophages had drugs from Fucus vesiculosus. These drugs have also been stimulating the step and intact animals.

For a more in-depth study of the impact of drugs on the functional activity of macrophages was used activity ectoplasmatic of the enzyme 5-nucleotidase. It is known that the activity of this enzyme is reduced in stages, the activation of macrophages. The enzyme activity was determined in the serum of mice CBA, which within 15 days is administered orally investigational drugs. The enzyme activity was determined spectrophotometrically using as substrates the AMP and glycerol.

As can be seen from table 17, stimulating effect on the activity of macrophages possess drugs from Fucus vesiculosus. Drugs from Ascophyllum nodosum affect the activity of macrophages. Thus, all the studied drugs do not exert immunotoxic action.

Studies on the action of the BAD on oxybiotic anti-infective system of phagocytes were performed on mice CBA, which orally for 15 days were injected drugs in the dose MTD 0.5 ml served as Control animals received the same volume of saline. Heparinised blood in experimental and control samples were mixed with CNT, incubated at 37°With, then part of the test and control samples were added opsonizing zymosan (enhanced version). In the recovery of HCT passes into the insoluble diformate that op is Adelaide spectrophotometrically in DNF at 670 nm.

Analyzing the effects of drugs on the factors of natural resistance, it can be concluded that the aqueous concentrate of Fucus vesiculosus increases the phagocytic activity. Drugs from Ascophyllum nodosum not have overwhelming action on nonspecific factors of protection.

Conclusion.

The results of studies of mass and cellularity of the Central and peripheral organs of the immune system, the humoral immune response to various antigens, functional activity of T-lymphocytes and macrophages and factors of nonspecific resistance of the organism of laboratory animals revealed no immunotoxic action none of the drugs studied. Drugs are not suppressed, and in some cases stimulated macrophage immunity.

Studies of the immunomodulatory activity of preparations of intact and immunodepression animals were allowed to select two drugs having immunostimulatory activity of drugs from Fucus vesiculosus (water concentrate and nibs), which had a stimulating effect PA humoral immunity and cell-posredovanje reactions and significantly increased macrophage immunity, increasing the functional activity of macrophages and neutrophilic granulocytes, stimulating oxybiotic anti-infective system of phagocytes as the intact animals, and the animals on the background of immunosuppression.

1.3.2.4. Study of the antimicrobial action of dietary SUPPLEMENTS.

Investigated water concentrates from Ascophyllum nodosum and Fucus vesiculosus. Studies were performed according to the State Pharmacopoeia of the USSR with the following test organisms Escherichia coli ATCC 32218, Bacillus subtilis ATCC 6533, Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 6538, Candida albicans No. 1080/330 for standard environments (meat-peptone agar and Saburo) by the method of diffusion in agar.

Determination was carried out by comparing the presence or absence of zones of inhibition of growth of microorganisms in the test dilutions of drugs and control, which used the drug Katapola. The tests were performed in three replications.

It is shown that the most pronounced direct antimicrobial action has water concentrate Fucus vesiculosus. Thus, the minimum inhibitory concentration (MIC) in relation to the culture of Staphylococcus aureus is 0.3 mg/ml against E. coli - 0.6 mg/ml Slightly less pronounced antimicrobial activity has the drug from Ascophyllum nodosum. Its IPC against Staphylococcus - 0.6 mg/ml against Escherichia coli - 2.5 mg/ml

The investigated drugs do not inhibit growth of cultures of Pseudomonas aeruginosa was ATSS, Candida albicans.

1.3.2.5. Study of the protective effect of the SUPPLEMENTS on the model of an infectious process.

Used a model of acute staphylococcal sepsis is, which was reproduced on white outbred mice introduction intraperitoneally culture of Staphylococcus aureus in a dose of 2 billion microbial cells in the mouse. The drug was administered for 6 days before the introduction of the culture and even 2 days after administration of oral culture.

The effectiveness of the SUPPLEMENTS was assessed by life expectancy and colonization of Staphylococcus renal tissue. Control animals received the drug did not enter. The second control was a group of animals who were injected tetracycline.

It is shown that the combined administration of drugs of Fucus vesiculosus and tetracycline has greatly increased the life expectancy of animals. Combined administration of drugs Fucus vesiculosus and antibiotics has led in some cases to significant reorganization of renal tissue from animals with staphylococcal sepsis (table 19). Several smaller protective effect possessed drugs from Ascophyllum nodosum. The obtained data demonstrate the ability of the investigated drugs in varying degrees to potentiate the activity of the antibiotic.

Evaluation of antioxidant action.

Assessing the impact of the investigated samples BAD on the parameters of oxidative-antioxidative system was performed on mice that daily oral drove the analyzed solutions of drugs in the dose 1/2 MTD. The model disturbances of lipid peroxidation reproduced introduction intramuscularly is about 0.25 ml oil 7% solution of carbon tetrachloride. During the experience was determined by the content of malondialdehyde, diene conjugates polemicising fatty acids and lipid hydroperoxides in liver homogenates /32/.

To determine the content of diene conjugates in the liver homogenate of mice in the buffer solution was extracted with a mixture of heptane and isopropyl alcohol in a ratio of 1:1. Then aliquots alcohol phase was mixed with ethanol at a ratio of 1:10. The optical density of the solutions was measured at 233 nm. The content of diene conjugates in the samples was calculated based on the values of molar extinction coefficient for paired diones polyunsaturated higher fatty acids, 2,2×105l/(mol×cm). The results were expressed in nmol/g of tissue (table).

For determination of lipid hydroperoxides in the liver homogenate of mice in the buffer solution was centrifuged and added to the supernatant ethanol, hydrochloric acid and 5% salt solution Mora. After 30 seconds, was added a 20% solution of ammonium thiocyanate. The optical density was measured for 10 min at 480 nm. The content of hydroperoxides in the samples was calculated based on the values of molar extinction coefficient for Rodenstock iron, different 6300 l/(mol×cm). The results were expressed in µmol/g tissue (table).

For the determination of radon dialdehyde to the liver homogenate m the necks in Tris-buffer was added 17% trichloroacetic acid and centrifuged. To the supernatant was added to 0.8% solution thiobarbiturate acid and the samples were placed for 10 min in a boiling water bath. After the development of pink color of the sample was cooled to room temperature and measured the optical density at 532 nm. The amount of malondialdehyde were determined using the value of the molar extinction coefficient trimethylboron complex formed by the interaction of the dialdehyde with thiobarbituric acid, equal to 1.56×103l/(mol×cm). The results were expressed in nmol/g of tissue (table).

As a result of the research (table) shows that the introduction of drugs to varying degrees reduces the levels of diene conjugates, hydroperoxides and malondialdehyde. The most pronounced reduction of diene conjugates appearing on the initial stages of lipid peroxidation and malondialdehyde (final product) for drugs Fucus vesiculosus. This, apparently, can be the basis for their use as antioxidants.

Study of the protective action of dietary SUPPLEMENTS.

We studied the effect of fucoidan on the survival of mice in pharmacological models, bacterial radiation immunosuppression.

Pharmacological immunosuppression was carried azathioprine scheme: 25 mg/kg orally three times, 50 mg/kg intraperitoneally once. Then animals hedgehog the day orally was administered investigational drugs in a dose of 0.5 MTD. The monitoring was conducted for two weeks.

Bacterial immunosuppression was reproduced in mice CBA introduction intraperitoneally culture of Staphylococcus aureus in a dose of 2 billion microbial cells in the mouse. Drugs at a dose of 0.5 MTD was administered for 8 days prior to the introduction of culture and 7 days after administration of the oral culture.

Experiments on the protective action of dietary SUPPLEMENTS for radiation immunosuppression was performed on CBA mice irradiated with dose of 600 R for machine ROOM-1 7. The drugs were injected, as described above, for 2 days before exposure on a daily basis.

As can be seen from table 21, all investigational drugs in different exert a protective effect on the organism of animals with immunosuppression of various origins, the greatest protective effect exerted drugs from Fucus vesiculosus, which indicates the possibility of using these drugs in the treatment of immunodeficiencies different nature.

1.3.2.7. Conclusions.

1. All investigated drugs no toxic action on the organism of laboratory animals in acute experiments and belong to the class of low-toxic substances.

2. General toxic properties of drugs with multiple introduction in the chronic experiment no. The drugs are well tolerated by the animals and do not cause violations of the functional state.

3. Drugs do not exert immunotoxic and allergenic de the damage.

4. Drugs from Fucus vesiculosus have a marked immunostimulating effect, increasing the functional activity of macrophages and neutrophilic granulocytes.

5. Studied drugs have a protective effect on the organism of animals with the simulated immunosuppression of various origins, and the protective effects of Fucus vesiculosus more pronounced.

6. The investigated drugs in varying degrees possess direct antimicrobial activity against Escherichia coli and Staphilococcus.

7. Studied drugs have a marked antioxidant effect in animal model with disturbances of lipid peroxidation.

1.3.3. The results of microbiological testing of products from fucoidan.

The objective of this study is the determination of the shelf life of products from fucoidan.

Samples of Fucus vesiculosus were received on March 20, and from Ascophyllum nodosum - March 26, 2001. Microbiological examination of samples of completed December 2001, i.e. after nine months of storage at room temperature.

Presents copies of the test show that the samples are safe and consistent with the requirements of the Sanpa and N 2.3.2.560-96 /33/ PP BAD sources mainly dietary fiber, PP. BAD sources mainly macro - and micronutrients and p. - historicalideological active substances (parapharmaceuticals) (table 22).

1.3.4. The rationale for the use of products from fucoidan as dietary supplements.

According to the RF law "On quality and safety of food products" from 2.01.2000, /5/ food additives and biologically active additives (BAA) relate to food. These two kinds of food products the law gives the following definition:

- food additives, natural or artificial substances and their compounds, specially inserted in the food products during their manufacture in order to give food to certain properties and / or maintain the quality of food products;

- biologically active additives - natural (identical to natural) biologically active substances intended for use concomitantly with food or in food products.

We used the solution of the extract "Fucus" in the treatment of inflammatory periodontal diseases.

Extract "Fucus" (TU 9284-001-00472035-2001) food (hereinafter referred to as the extract derived from brown algae Ascophyllum nodosum and Fucus vesiculosus. The extract is obtained by extraction of air-dried algae ethanol with subsequent removal of extrogen.

The extract contains polysaccharides, mannitol, calcium, magnesium, potassium, sodium, zinc, trace minerals and iodine. Intended for use as food SUPPLEMENTS to fill dietary intake of iodine, carbohydrate is mi, macro - and microelements. Its use is indicated for diseases of the cardiovascular system, atherosclerosis, low productivity and a weakened immune system, as well as under adverse factors.

The technical requirements.

1. Extract "Fucus" food (the extract) must comply with this specification and produced according to the current technical instructions with the observance of sanitary norms and rules, as approved in the prescribed manner.

2. Raw materials for the manufacture of the extract are marine brown algae Fucus vesiculosus and Ascophyllum nodosum.

3. Characteristics.

On the organoleptic, physico-chemical and microbiological parameters extract must meet the requirements specified in table 1, 2.

Requirements for raw materials and resources.

Raw materials used for making the extract must meet the following requirements:

1. Brown algae Fucus vesiculosus and Ascophyllum nodosum - TU 9284-023-00462769-2001.

2. Drinking water GOST 2874.

3. Ethyl alcohol - GOST 5962, GOST 5963.

The study of subacute toxicity.

We applied a 1% solution of the extract "Fucus". This concentration is selected on the basis of the crystallographic method for the study of biological substrates. The nature of crystallophobia there are two types of microcrystallization saliva. When you first type of MIC is crystallizatio mixed with saliva characteristic in view of the large tree crystal-like formation, located in the centre of the drop. Organic matter is located in a small quantity on the periphery of the drop. The second type of microcrystallization in the field of view visible single crystal-like conglomerates or needle crystals, spaced evenly across the field, or have a tendency to grouping on the periphery of the drop.

Research solutions extract 0,5%, 1%, 2%, 3% concentrations showed that the crystallographic picture of 0.5% and 1% solutions of the characteristic of the first type of microcrystallization, i.e. similar to the mixed saliva of a person with the ability to ensure the homeostasis of mineral components in the oral cavity.

For crystallographic picture of 2% and 3% typical second type of microcrystallization.

Thus, experimental studies allowed to choose 1% solution of the extract, which compared to the other solutions of concentrations has good kristalloobrazuyushchikh ability, which is similar to the mixed saliva with good mineralizing potential.

Our aim is to identify the effectiveness of the extract in the treatment of periodontal diseases.

To achieve this goal the following tasks were defined:

- To evaluate the anti-inflammatory efficacy of the extract "Fucus".

To study the effect of solution extrac is and on the state of the microflora of periodontal pockets.

When solving tasks were formed three groups of subjects aged from 15 to 20 years. The first group consisted of 32 people, two other control, for 10 people.

To assess the clinical and hygienic condition of the mouth used the following indexes:

hygiene index OHI-S (Greene., Vermillion., 1964).

- the index of the RMA (Parma S., 1960);

the index PI (Russel A., 1956);

index, bleeding on Muhlemann H.P.

Before treatment, patients were trained to care for the oral cavity, conducted professional hygiene of the oral cavity, including the removal of dental plaque from grinding and polishing of the necks and roots of the teeth, oral cavity sanation. Evaluation of anti-inflammatory action was carried out on the basis of changes of periodontal indices, which are determined at the control examinations before and after treatment.

Reduced salivation and high viscosity of saliva associated with the violation of the self-purification process in the oral cavity. In this regard, we have carried out the determination of the viscosity of the saliva and the salivary flow rate. Determination of the viscosity was carried out on removeconsumer capillary VC-4.

The first group was treated with a solution of an extract of "the Family". The other two, the control, received treatment chlorhexidine 0.1 % solution of tincture of calendula.

The treatment consisted in apliciranje process is economical for 20 minutes, the number of sessions was 10. The results obtained are presented in table 21.

As can be seen from table 21, the index of the ACA before treatment was 43.7%±0.8, PI - 2.3±0.06, bleeding index 2.8±0.05, the viscosity of the saliva 5.3±0.5, the rate of salivary flow 0.2±0.01, after treatment with the extract "Fucus" index ACA amounted to 9.2%±1.0, index PEE - 0.4±0.03, bleeding index of 0.4±0.05, the viscosity of the saliva 2.0±0.01 the salivary flow rate 0.3±0.02. Improved hygienic condition of the mouth of the examined patients before treatment hygiene index was 1.9±0.1, and after treatment indicators have markedly decreased to 1.0±0.08.

After the medical students noted increased comfort in the mouth and the absence of bleeding while brushing your teeth, gums bought a pale pink color, fits close to the necks of the teeth.

Microbiological investigation was to study the qualitative (species) composition of the periodontal pocket. Taking content were conducted in the morning, on an empty stomach, before tooth brushing procedure. The study was conducted before and after the treatment.

The basis for evaluation of results of treatment were based on features of the species composition of the microflora of periodontal pockets. Using extract "Fucus" in complex treatment of periodontitis according to BA the mammalogical research reduces periodontopathogenic microorganisms. This is confirmed by the data analysis in the frequency of occurrence of major periodontopathogenic microorganisms of the actinomycetes - gram-positive anaerobic rods and pathogenic streptococcal species. In addition, expressed significantly positive changes related to the gram-negative actinobacter and conditionally pathogenic enterobacteria defined after treatment.

Of course, a positive result after treatment, the appearance of lactic acid microorganisms. The absence of lactic acid bacteria in periodontal pockets of patients with periodontitis indicates a violation of the colonization resistance of the organs of the mouth, i.e. resistance to colonization random and pathogenic for this ecological niches microorganisms. The appearance of lactic acid microorganisms after treatment involves the inhibitory mechanisms of their influence on conditionally pathogenic and pathogenic flora of periodontal pockets. The results of microbiological studies are presented in table 22.

The analysis of the dynamics of clinical and physiological and microbiological parameters of periodontal status showed that a 1% solution of the extract Fucus is effective in the prevention of disadaptation changes in the organs and tissues of the oral cavity. We have found the fact that reduction values periodontal index provides is to say, this drug is effective in the treatment of inflammatory periodontal diseases.

Literature

1. Kotlova O. Sokolova, T.A., Khramtsova H.E. Treatment of inflammatory periodontal diseases algal drugs. #Main dental diseases, their treatment and prevention in the European North. - Arkhangelsk, 1998, Pp.31-33.

2. Pravin A.S., A.A. Fokin, Leoshkina E.V. Experimental substantiation of the anticariogenic effect of water-salt solution concentrate kelp. #Main dental diseases, their treatment and prevention in the European North. - Arkhangelsk, 1996. - P.48-50.

3. Levitskaya E.V., Sokolovsky H.E. the Use of drugs of plant origin with periodontitis. # Therapeutic dentistry. - Kiev, 1976. - VIP. - S-75.

4. Kodola N.A. Application of calendula in the treatment of inflammatory and degenerative forms of periodontal disease. # Abstr. total. obeisty. scient. proc. Kiev Institute of improvement of doctors. - Kiev, 1960.

5. Pozharitskaya M.M., Sidra SR Methods of traditional medicine and the prospect of application in dental practice. # New in dentistry: Spec. vol. - 1994. No. 1. - C.11.

6. Order T.M., Dobrodeev L.K., Frantsuzova EL, Panicheva L.A. the Effectiveness of treatment of periodontitis products from algae.

7. Bregu-M; Mearns-Spragg-A. Cross-species induction and enhancement of animicrobial activity produced by epibiotic bacteria from marine algae and invertebrates, after exposure to terrestrial bacteria. # Lett-Appl-Environ. - 1998 Sep; 27 (3); 142-6.

8. Fabregas-J; Munoz-A; Llovo-J. Differentiation of Candida guilliermondii yeast varieties by lectin-like substances from marine algae. # Res-Environ. - 1989, Jul-Aug; 140 (6), 373-8.

Table 1.

Acute toxicity (LD50BAD
Samples BAARoute of administration
oral, mg/kgintraperitoneal, mg/kg
Water concentrate Ascophyllum nodosumno death19500
Nibs from Ascophyllum nodosum23442000
Water concentrate Fucus vesiculosus1925014000
Nibs from Fucus vesiculosus33342250
Table 2.

Dynamics of body mass (in g) of Guinea pigs in the study of subacute toxicity of dietary SUPPLEMENTS.
Samples BAAThe follow-up period
Background2 weeks1 month
Control 285,50±10,11310,60±12,80338,00±14,30
Water concentrate Ascophyl. nodosum306,25±12,50325,50±17,10345,45±17,20
Nibs from Ascoph. nodosum.290,00±10,45318,65±14,00340,85±13,50
Water conc t Fucus vesiculosus301,10±11,90320,00±13,20341,50±12,35
Nibs from Fucus vesiculosus288,10±11,20315,55±12,20334,10±14,25

Table 3.

The clinical analysis of blood of Guinea pigs treated with dietary SUPPLEMENTS.
The observation periodMedication
ControlWater conc t Ascoph.nodosumNibs from Ascoph.nodosumWater conc t Fucus vesiculosusNibs from Fucus vesiculosus
The number of leukocytes, 109/ l
Background5,81±0,625,52±0,52 6,01±0,605,52±0,505,40±0,56
1 monththe ceiling of 5.60±0,516,20±0,616,28±0,71the ceiling of 5.60±0,405,77±0,42
The number of erythrocytes, 1012/l
Background4,95±0,144,63±0,304,80±0,314,75±0,154,85±0,50
1 month4,60±0,315,06±0,154,95±0,154,63±0,145,52±0,33
Hemoglobin, g/l
Background126,20±2,80125,83±8,06128,90±2,85130,80±4,30136,24±3,45
1 month127,40±3,40128,50±2,90132,32±3,40129,00±2,39138,33±3,39
Hematocrit (l/l
Background0,385±0,02 0,423±0,0300,375±0,0200,420±0,0200,400±0,050
1 month0,435±0,0200,365±0,0210,410±0,0300,385±0,0350,492±0,010
The average volume of erythrocytes, FL
Background89,82±0,7591,83±0,6590,82±0,6089,75±0,8592,71±0,56
1 month91,90±0,7190,67±0,9791,50±0,6490,10±0,9088,83±0,81
The average amount of hemoglobin in erythrocytes, PG
Background25,56±0,5126,28±0,3626,55±0,5125,68±0,3425,62±0,51
1 month26,11±0,3125,48±0,4425,76±0,3327,47±0,42of 24.90±0,29
The average concentration in one erythrocyte, g/l
Background282,50±6,12297,17±5,77290,50±5,00295,45±4,20286,50±4,18
1 month296,55±to 5.21280,50±5,16296,55±6,20289,80±6,10281,00±5,97

Table 4.

Indicators of creatinine and urea (mmol/l) in blood of Guinea pigs treated with dietary SUPPLEMENTS.
The observation periodMedication
ControlWater conc t Ascoph.nodosumNibs from Ascoph. nodosumWater conc t Fucus vesiculosusNibs from Fucus vesiculosus
Creatinine
Backgroundto 0.060±0,0010,070±0,0010,065±0,0020,065±0,0010,065±0,001
1 month0,058±0,0020,080±0,0010,070±0,001 0,170±0,0010,070±0,001
Urea
Background10,55±0,1110,54±0,1210,02±0,1110,55±0,1110,00±0,10
1 month10,10±0,21of 10.58±0,1010,31±0,1311,02±0,1110,55±0,11

Table 5.

Indicators of enzyme activity (U/l) serum of Guinea pigs treated with the BAD
The observation periodMedication
ControlWater concentrate-t Ascophyllum nodosumNibs from Ascophyllum nodosumWater concentration-t Fucus vesiculosusNibs from Fucus vesiculosus
Alkaline phosphatase
Background211,43±12,10206,14±or 10.60193,95±9,60217,30±10,20206,15±br12.62
1 month202,55±12,20198,26±11,80201,00±10,12211,15±11,12195,46±11,82
Aminotransferase
Background64,74±4,2171,12±3,7468,88±3,4570.62 per±2,4071,40±4,80
1 month67,35±of 5.4069,95±4,1169,10±5,1069,11±5,2076,67±5,00
Aspartate aminotransferase
Background142,25±11,50148,60±11,40144,30±15,10146,70±10,11138,60±the 10.40
1 month145,64±11,88153,22±12,42150,12±11,75151,21±9,70142,25±11,50

Table 6.
NameMedication
bodyControlWater concentrate-t Ascophyllum nodosumNibs from Ascophyllum nodosumWater concentration-t Fucus vesiculosusNibs from Fucus vesiculosus
Liver4,053,753,823,963,90
Kidney0,910,900,900,890,91
Spleen0,300,280,280,270,29
Heart0,400,390,400,380,39
Light1,000,960,970,940,95

Table 7.

The results of the study of blood sera method RDTC.
Timing of serum, daysMedication
Water concentrate-t Ascophyllum nodosumNibs from Ascophyllum nodosumWater concentration-t Fucus vesiculosusNibs from Fucus vesiculosus
00,09±0,020,12±0,010,10±0,010,10±0,01
70,11±0,010,13±0,010,12±0,010,11±0,02
140,09±0,010,11±0,020,10±0,010,10±0,02
210,09±0,010,12±0,010,11±0,010,11±0,01

Table 8.

Effects of drugs on mass and cellularity of the organs of the immune system.
Mass (mg) and organ cellularity (cells/g ×108Medication
ControlWater concentrate-t Ascophyllum nodosumNibs from Ascophyllum nodosumWater concentration-t Fucus vesiculosusNibs from Fucus vesiculosus
Spleen
Weight99,4±4,7to 102.3±5,1

p>0,05
110,5±4,8

p>0,05
97,0±3,5

p>0,05
115,6±3,9

p>0,05
Cellularity11,5±0,712,2±0,4

p>0,05
14,9±0,3

p>0,05
11,8±0,3

p>0,05
13,9±0,6

p>0,05
The thymus
Weight46,2±2,348,6±1,9

p>0,05
50,1±3,2

p>0,05
45,8±2,7

p>0,05
47,3±2,0

p>0,05
Cellularity10,2±0,49,7±0,3

p>0,05
a 9.5±0,3

p>0,05
10,5±0,5

p>0,05
9,9±0,4

p>0,05
Lymph nodes
Weight25,1±1,123,8±1,3

p>0,05
24,0±0,9

p>0,05
22,9±1,1

p>0,05
23,4±1,2

p>0,05
Cellularity6,3±0,25,8±0,3

p>0,05
6,1±0,2

p>0,05
5,9±0,1

p>0,05
6,0±0,2

p>0,05
Note: p is the significance level of differences compared to control animals.

Nibs from Ascophyllum nodosum
Table 9.

A BAD influence on humoral immune response.
DrugsThe average logarithm base two of the hemagglutinin titers timing (day)
7142128
Control6,36,37,36,3
Water concentrate-t Ascophyllum nodosum6,36,38,36,3
6,36,37,36,3
Water concentration-t Fucus vesiculosus6,39,38,36,3
Nibs from Fucus vesiculosus6,37,37,36,3

Table 10.

The influence of additives on the synthesis of antibodies of class Ig M
MedicationThe number AOK 106the splenocytes
Control240±18
Water concentrate-t Ascophyllum nodosum256±11 (p>0,05)
Nibs from Ascophyllum nodosum249±15 (p>0,05)
Water concentration-t Fucus vesiculosus292±16 (p<0,05)
Nibs from Fucus vesiculosus283±14 (p<0,05)
Note: p is the significance level of differences compared to control animals.

Table 11.

The BAD influence on the development of the reaction GST.
MedicationThe number of animalsIndex reactionp
Control30 4,25±0,37>0,05
Water concentrate-t Ascophyllum nodosum304,05±0,40>0,05
Nibs from Ascophyllum nodosum30of 4.44±0,43>0,05
Water concentration-t Fucus vesiculosus304,12±0,28>0,05
Nibs from Fucus vesiculosus304,32±0,39>0,05
Note: p is the significance level of differences compared to control animals.
Table 12.

The BAD influence on the migration of lymphocytes.
MedicationPumas leukocyte %
Tuberculin, 20 mg/mlTuberculin, 40 mg/ml
Control1012
Water concentrate-t Ascophyllum nodosum1621
Nibs from Ascophyllum nodosum2024
Water is ancient-t Fucus vesiculosus 3231
Nibs from Fucus vesiculosus3336

Table 13.

Determination of phagocytic activity of macrophages in the test with neutral red.
Samples BAAThe optical density at 540 nm
Intact mouseImmunodepression mouse
Control0,41±0,020,30±0,02
Water concentrate-t Ascophyllum nodosum0,39±0,010,33±0,01
Nibs from Ascophyllum nodosum0,44±0,020,46±0,02
Water concentration-t Fucus vesiculosus0,64±0,020,60±0,01
Nibs from Fucus vesiculosus0,42±0,020,48±0,01
Table 14.

The influence of additives on the activity of 5 - nucleotidase serum.
Samples BAAThe content of inorganic phosphorus, mg/ml% suppression activity
Pin the ol 16,00±0,52
Water concentrate-t Ascophyllum nodosum14,60±0,748
Nibs from Ascophyllum nodosum14,60±0,658
Water concentration-t Fucus vesiculosus10,13±0,6838
Nibs from Fucus vesiculosus12,14±0,6425

Table 15.

To determine the impact of the BAD factors of nonspecific resistance in the test recovery of HCT.
Samples BAASpontaneous variantStimulated option
Control0,54±0,020,55±0,03
Water concentrate-t Ascophyllum nodosum0,58±0,030,52±0,03
Nibs from Ascophyllum nodosum0,50±0,020,54±0,03
Water concentration-t Fucus vesiculosus0,70±0,020,75±0,02
Nibs from Fucus vesiculosus0,62±0,030,59±0,03
Table 16.

Effects the drugs for infection of the tissues of the kidneys laboratory when staphylococcal sepsis.
DrugsThe number of colonies per 1 g tissue kidney
Control(183,5±20,2)×103
Water concentration-t Fucus vesiculosus(90,2±12,4)×103
Nibs from Fucus vesiculosus(98,3±10,7)×103
Tetracycline(45,4±6,2)×103
Tetracycline + aq. concentrator-t Fucus vesiculosus(60,0±30,1)×103

Table 17.

The BAD influence on the parameters of oxidative and antioxidative activity.
Samples BAAThe content in the liver tissue
Diene conjugates, nmol/gThe gidroperekisi lipids, mmol/gMalonic dialdehyde, nmol/g
Control211,0±8,22,83±0,0817,7±1,1
Water130,3±6,9 2,60±0,1011,7±0,5
concentrator-t Fucus vesiculosusp<0,05p>0,05p<0,05
The grains of Fucus117,8±6,12,70±0,1010,4±0,1
vesiculosusp<0,05p>0,05p<0,05
Tetracycline119,2±5,52,85±0,1212,5±0,6
p<0,05p>0,05p<0,05
Tetracycline +to 106.0+2,12,30±0,1812,7±0,8
bodi. concentrator-t Fucus vesiculosusp<0,05p>0,05p<0,05
Table 18.

Protective effect of SUPPLEMENTS on models immunodepressed various origins.
Samples BAAThe increased survival of animals, % of control
Pharmacologic. the immunosuppression Bacterial immunosuppressionRadiationa the immunosuppression
Water concentrate-t Ascophyllum nodosum29,233,115,8
Nibs from Ascophyllum nodosum26,428,417,9
Water concentration-t Fucus vesiculosus43,553,733,2
Nibs from Fucus vesiculosus38,141,525,1

Table 19.
Name of indicatorCharacteristics and NormaTest method
AppearanceOpaque liquidGOST 20438 section 3.1.
ColorDark brownGOST 20438 3.2.
SmellSpecific characteristic of this productGOST 20438 clause 3.3.
Mass fraction of water,%, no more50GOST 26185 3.2.
Mass fraction of ash, % dry product, no more than50GOST 26185 clause 3.3.
the pH of the extract3,5-5,0GOST 26185 paragraph 4.3.
MineralGOST 30504
elements, g/DMGOST 30503
potassium20-30GOST 26570
sodium40-60RD 52.24.403-95
calcium0,4-0,7RD 52.24.395-95
magnesium0,3-0,8

/tr>
Table 20.
Name of indicatorCharacterization and NormaTest method
MicrobiologyInstruction
indicators:sanitary-
The number of mesophilicMicrobiology
aerobic andcontrol
optional-production
anaerobicfood products
microorganisms, SOMEfish and sea
1 g, not more mass, ininvertebrates
which is not allowed1×104(Approved
g:Roskomnadzor
BGK (coliforms)CCCP 22.02.91, No. 5
pathogenic, including0,1319-91)
Salmonella mold10
mushrooms, CFU in 1 g, no
more
100
Toxic elements,GOST 26927-26935
mg/kg dry mass, notRaw materials and products
morefood. Methods
lead6,0define
cadmium1,0toxic elements.
arsenicto 12.0
mercury0,1
Shelf life, year1,0MU 3.2.727-99
Hygienic assessment
shelf life
&x0200A; food.

Table 21.
PIPMAGIIRThe viscosity of the salivaThe rate of salivary flow
Extract "Fucus"2,3±0,060,4±0,0343,7±0,89,2±1,01,9±0,10,9±0,081,6±0,050,5±0,15,3±0,52,0±0,20,2±0,010,3±0,02
of 0.1%p-p of tincture of calendula1,8±0,40,6±0,0944,2±0,813,6±1,01,8±0,20,9±0,11,1±0,21,1±0,25,8±0,73,1±0,50,2±0,010,2±0,03
R-R hlorgeksedina2,04±0,40,7±0,239,8±0,113,6±0,12,1±0,11,5±0,11,0±0,30,9±0,15,6±0,92,0±0,20,25±0,0303± 0,03

Table 22.

The DYNAMICS of MICROBIOLOGICAL INDICATORS of the oral CAVITY IN the TREATMENT SOLUTION of the EXTRACT "FUCUS" AMONG RESIDENTS, ARKHANGELSK
The composition of the microflora (%)Before the treatmentAfter the treatmentThe control group
Before the treatmentAfter the treatment
Candida albicans4,5±0,752,3±0,1202,6±0,91
Str.mutans4,6±1,322,5±0,767,2±0,876,1±0,89
At all6,3±0,75000
Actinomyces7,3±0,8105,0±2,45,8±2,3
Corynebacterium1,5±1,821,1±1,811,1±1,652,1±0,96
Lactobacteria, lactococcus15,5±0,3254,2±0,1434,21±0,3238,6±0,54
We did Haemophilus1,5±0,3200,7±1,90,7±2,13
St. aureus1,2±0,90,7±1, 00,4±1,5
Note: R* - differences are significant in relation to the performance before the treatment

Table 23
PIPMAGIIRThe viscosity of the salivaThe rate of salivary flow
Extract"Fucus"2,3±0,060,4±0,0343,7±0,89,2±1,01,9±0,11,0±0,081,6±0,050,5±0,15,3±0,52,0±0,20,2±0,010,3±0,02
of 0.1%p-p of tincture of calendula1,8± 0,40,6±0,0944,2±0,813,6±1,01,8±0,20,9±0,11,1±0,21,1±0,25,8±0,73,1±0,50,2±0,010,2±0,03
R-R hlorgeksedina2,04±0,40,7±0,239,8±0,113,6±0,12,1±0,11,5±0,11,0±0,30,9#x000B1; 0,15,6±0,92,0±0,20,25±0,030,3±0,03

Composition for treatment of inflammatory periodontal diseases on the basis of brown algae, characterized in that the composition includes an extract "Fucus"derived from algae Ascophylum nodosum and Fucus vesiculosus and water in the following ratio of components, g:

Extract "Fucus"1
Water100



 

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EFFECT: higher efficiency.

FIELD: medicine, in particular agent for hand cleaning without water application.

SUBSTANCE: claimed agent contains polyvinyl alcohol bearing 8-13 of acetate groups and having dynamic viscosity from 23 to 47 Pa.c.103, or mixture of polyvinyl alcohol bearing 0.8-2.0 of acetate groups and having dynamic viscosity from 17 to 25 Pa.c.103 with polyvinyl alcohol bearing 8-13 of acetate groups and having dynamic viscosity from 16 to 19 Pa.c.103 in ratio of 1:(0.4-1.5), ethanol and water. Additionally agent contains PEG-40 and hydrogenised castor oil in specific ratio (mass %).

EFFECT: agent for hand cleaning applicable at minus temperatures without losses of consumption properties.

8 cl, 2 ex

FIELD: antiperspirant deodorant products.

SUBSTANCE: claimed product contains salt with antiperspirant activity and water soluble polymer, containing Bronsted acids, which acts as co-thickener when blending with antiperspirant salt in presence of water. Before application polymer is physically divided from antiperspirant salt.

EFFECT: antiperspirant product of improved effectiveness.

21 cl, 7 ex, 7 tbl

FIELD: cosmetology, in particular skin care products.

SUBSTANCE: claimed agent contains bioactive components, namely albumin, placenta hydrolyzate, and sodium alginate. Additionally it may contain target additives, glycerol and essential oils. All components are used in specific qualitative ratio.

EFFECT: cosmetic product of antimicrobial, moistening, stimulating, antiallergic, and sorption action, capable of stimulation repairer and smoothing processes.

2 cl, 8 ex

FIELD: cosmetology, in particular skin care products.

SUBSTANCE: claimed agent contains bioactive components, namely albumin, placenta hydrolyzate, and sodium alginate. Additionally it may contain target additives, glycerol and essential oils. All components are used in specific qualitative ratio.

EFFECT: cosmetic product of antimicrobial, moistening, stimulating, antiallergic, and sorption action, capable of stimulation repairer and smoothing processes.

2 cl, 8 ex

FIELD: cosmetic industry, in particular cosmetic agent for skin protection.

SUBSTANCE: claimed agent contains vitamin A compound and/or derivatives thereof, quinothiol, epidermal lipid and/or analog thereof selected from group containing sterols and/or derivatives thereof, sphingolipids and/or derivatives thereof, phospholipids and/or derivatives thereof, and glycerides and/or derivatives thereof, as well as desired additive. All components are used in specific ratio.

EFFECT: agent to improve dry skin state, to prevent corrugation formation and similar defects caused by losses of skin elasticity and resiliency.

2 cl, 6 ex, 5 tbl

FIELD: cosmetic industry, in particular cosmetic agent for skin protection.

SUBSTANCE: claimed agent contains vitamin A compound and/or derivatives thereof, quinothiol, epidermal lipid and/or analog thereof selected from group containing sterols and/or derivatives thereof, sphingolipids and/or derivatives thereof, phospholipids and/or derivatives thereof, and glycerides and/or derivatives thereof, as well as desired additive. All components are used in specific ratio.

EFFECT: agent to improve dry skin state, to prevent corrugation formation and similar defects caused by losses of skin elasticity and resiliency.

2 cl, 6 ex, 5 tbl

FIELD: agents for skin care, injury repair, and skin protection from harmful environment factors and pathogenic organisms.

SUBSTANCE: claimed agent contains polyethylene glycol of high polymerization degree n = 32-800, glycerol, lanthanum nitrate, sodium hydroxide, and water, and additionally it contains Panavir (new Russian antiviral drug), polyethylene glycol of polymerization degree n = 4-8. All components are used in specific quantitative ratio.

EFFECT: agent of improved properties, namely homogeneity and rheological properties, and increased applicability.

2 cl, 2 ex, 1 tbl

FIELD: medicine, dermatology, pharmaceutical industry, phytotherapy.

SUBSTANCE: invention relates to using extracts from Serenoa repens and Vitis vinifera for preparing oral compositions used in treatment and prophylaxis of alopecia, dandruff and seborrhea. Oral compositions prepared of extracts of Serenoa repens and Vitis vinifera promote to the effective treatment and prophylaxis of alopecia, dandruff and seborrhea. Invention is used in treatment and prophylaxis of alopecia, dandruff and seborrhea.

EFFECT: valuable medicinal properties of compositions.

4 tbl, 2 ex

FIELD: medicine, dermatology, pharmaceutical industry, phytotherapy.

SUBSTANCE: invention relates to using extracts from Serenoa repens and Vitis vinifera for preparing oral compositions used in treatment and prophylaxis of alopecia, dandruff and seborrhea. Oral compositions prepared of extracts of Serenoa repens and Vitis vinifera promote to the effective treatment and prophylaxis of alopecia, dandruff and seborrhea. Invention is used in treatment and prophylaxis of alopecia, dandruff and seborrhea.

EFFECT: valuable medicinal properties of compositions.

4 tbl, 2 ex

FIELD: medicine, oncology, amino acids.

SUBSTANCE: invention relates, in particular, to the development of an antitumor preparation based on natural substances. Invention relates to an amino acid preparation comprising at least one modified essential amino acid obtained by treatment of amino acid by ultraviolet radiation (UV) at wavelength 250-350 nm for 12-80 h at temperature 15-30oC or with ozone at temperature 15-25oC. The modified amino acid has no toxicity for health cells. Also, invention relates to a method for preparing such preparation. Invention provides the development of an antitumor preparation based on modified amino acids and expanded assortment of antitumor preparations being without cytotoxicity for normal cells.

EFFECT: valuable medicinal antitumor properties of preparation.

8 cl, 4 tbl, 2 dwg, 4 ex

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