Biologically active phospholipid-comprising complex for treatment of psoriasis and chronic prostatitis
SUBSTANCE: invention proposes the biologically active complex comprising phospholipids and the following biologically active substances - fatty acids, serines, peptides, amino acids, vitamins trace elements and extraction from cattle testicles prepared by successive fractionation with carbon dioxide and ethyl alcohol. The complex provides expanding spectrum of medicinal agents used in dermatological and urological practice and improving quality of the preparation. Invention can be used in treatment of psoriasis, prostatitis caused by prostatitis of sexual disorders.
EFFECT: valuable medicinal properties of complex.
The invention relates to medicine and can be used for the treatment of psoriasis, prostatitis and is caused by prostatitis sexual disorders.
A known number of integrated medicines containing phospholipids as the main active substance together with the other ingredients of plant, animal, or obtained by chemical means.
These drugs include Lipotherm, LIPOSTABIL, Aminophosphate (1), and Lipofundin (2), Intralipid (3), table 4).
Common to all these products is the presence of phospholipids as main active substance. The presence of phospholipids determines the main direction of action of drug - regulation of intracellular processes at the level of membranes. Functional significance of phospholipids based on their biphasic nature that allows you to adjust the permeability of the lipid layer of biological membranes, to restore its physico-chemical properties and to create adequate conditions for the functioning of enzymes. Phospholipids have antioxidant properties.
As a prototype of the selected remedy Essentiale produced in Germany. Essentiale (Esseutiale) are produced in two dosage forms : injection solution and capsules. The product contains about 70% of natural phospholipids (the source not specified) in the form diglycerin esters holinesterzoy acid, extract from soybean seeds with a high content of unsaturated fatty acids and added features such as tallow, soybean oil, hydrogenated, Kastoria oil, ethanol, ethylvanillin, 4-methoxyacetophenone, gelatin, dye E 171, E 172, DL-a-tocopherol. Indications for use Essentiale are chronic diseases of the liver, fatty degeneration and hepatic toxicity, psoriasis, radiation syndrome.
The aim of the invention is the expansion of the range of medicines in dermatological and urological practice, improving the quality of the drug.
The invention consists in using a new integrated product containing phospholipids and such biologically active components - fatty acids, sterols, peptides, amino acids, vitamins, microelements for the treatment of psoriasis, prostatitis and is caused by prostatitis sexual disorders in men. Listed all complex substances in the composition of the drug called Stimutacs presents natural biologically active substances isolated from bovine testis. The tissue of the testes of cattle, purified from dietary lipids by extraction with carbon dioxide, extracted with a solution of ethyl alcohol. Alcohol extract was separated by centrifugation. Centrifugal evaporated.
The complex contains the inhabitants of natural biologically active substances in the following quantities (mass.% of dry matter):
|including free higher fatty|
|acids and sterols|
|nitrogen-containing substances, including||20,0±4,0|
|peptides and amino acids|
|vitamins, micro and macro-elements||6,3|
The complex of biologically active substances in the composition of the preparation, it provides high efficiency, because the effect of phospholipids combined and supplemented by the action of low molecular weight peptides, which play the role of bioregulators. Fatty acids, particularly arachidonic, linoleic are biogenic precursors of prostaglandins involved in the regulation of cellular metabolism and affect the circulation, including circulation.
Amino acids that are part of the complex, stimulate metabolic processes, as the plastic material for the synthesis of protein, and vitamins and minerals to enhance the antioxidant properties of phospholipids and enzymes. The synergy of the components of a comprehensive drug Stimutacs determines the high efficiency of its actions.
P is Torinese number of diseases has been a violation of lipid metabolism, in these cases used for the treatment of drugs normalize this process, including preparations containing phospholipids. So taken as a prototype Essentiale successfully used for the treatment of psoriasis. The course of complex treatment with this drug is not less than 3 months. Essentiale is used in two dosage forms. The solution for injection is administered 2-4 capsules per day for 10 days, the contents of the ampoule is diluted by the blood of the patient in the ratio of 1:1. Before intravenous infusion of Essentiale prescribed a two-week course of treatment by taking 2 capsules 3 times a day, and after intravenous infusion to continue taking the capsules for 2 months. The cost of a course of drug treatment is high, and therefore its use in hospitals is very limited. The positive effect of drugs containing phospholipids indicated in the treatment of prostatitis. Lipids, neutral fats, phospholipids and cholesterol are an important component of the prostate gland secretion. The main part of the phospholipids - phosphatidylcholine (lecithin) is a permanent component of the cellular structure of membranes defining their permeability. A large number of lecithin seeds in the prostate fluid is an indicator of its healthy condition (6).
Based on these data, was conducted clinical testing of a new pre is Arata Stimutacs two clinics - Nizhny Novgorod research dermatovenerologic Institute in the Department of chronic dermatoses and in the regional hospital. N. A. Semashko. For clinical study was prepared two dosage forms of the drug - suppositories and lyophilized powder. Suppositories were prepared on the basis of Witepsol N-15 and W-35 conventional for the manufacture of this drug form technology. The weight of the suppository was 2 g, and the content in suppositories active ingredient equal to (150±10) mg For the preparation of freeze-dried form Stimutacs was suspensible in water to a concentration (600±50) mg %, the suspension was poured into vials of 10 and 25 cm3and subjected to freeze drying uniform heat on the shelves of the freeze installation 5°per hour at a pressure of 0.03 Top and condenser temperature of minus 50±2)°C. the Duration of drying was equal 22-26 hours, the residual moisture of the product is not exceeded (3,0±0,5)%.
The essential difference between the comprehensive drug Stimutacs from the prototype is the following:
1) presents complex of biologically active components extracted from natural raw materials of the original method;
2) with the exception of phospholipids all other ingredients not repeat composition Essentiale;
3) Stimutacs used in other l the drug forms - suppositories and lyophilized powder.
The advantage of the prototype is as follows:
1) dosage form of the drug in the form of suppositories promotes rapid release of drug substances from the basics. Due to the high suction capacity of the mucosa of the rectum quickly achieved a high concentration of drug in the blood. Due to the deposition of the barrier function of the liver increases the duration of circulation of medicinal substances in the blood line;
2) suppozitornyj form allows you to eliminate the disadvantages of injecting drug - route of administration is painless and does not require skilled health personnel;
3) there is no possibility of infection of the patient;
4) the absence of drug chemical substances, fillers, colorants, stabilizers;
5) balanced complex of natural substances, providing the synergy of their actions, selected from the same source of raw materials - bovine testis;
6) availability of the drug due to its lower cost and domestic production;
and, as follows from the below data, clinical trials,
7) indications for use for prostatitis, missing in the testimony for the prototype;
8) a shorter course of treatment.
Before conducting the testing Nor gorodski Institute of hygiene and occupational pathology was conducted expert evaluation of the toxicity of a new drug. Experimental Toxicological studies have shown that the drug indicators of acute toxicity, cumulation coefficient, irritating, sensitizing and obscherezorbtivnymi action complies with the safety requirements to pharmacological agents.
The effectiveness of Stimutacs in the treatment of patients with psoriasis was conducted in the Department of chronic dermatoses, NIKWI. The group of patients consisted of 5 women and 7 men aged from 18 to 47 years. All patients had widespread psoriasis in the acute stage of the process. Stimutacs applied rectally in suppository 1 time a day for 15-30 days. Before and after treatment the study was conducted General blood analysis, urine analysis, serum transaminase, lipid, phospholipid spectrum of serum and erythrocyte membranes.
A list of patients receiving Stimutacs
|No.||NAME of the patient||no history of the disease|
All patients tolerated the drug Stimutacs well. Complications and side effects have been recorded. All patients with psoriasis treated with Stimutacs, after 7-10 days showed a significant improvement in the clinical picture of the disease in the form of stopping peeling, reducing the brightness of the rash and reduce air infiltration in homes. In 6 patients the previous exacerbation of psoriasis were stopped only by the use of cytotoxic drugs (methotrexate), with severe adverse side effects. Using Stimutacs in this group of patients has allowed to achieve remission without the use of methotrexate.
In the process of therapy Climatecam not been adverse shifts from General blood analysis, urine analysis, serum transaminase. In the study of lipid metabolism has been shown that Stimate is not substantially affect the level of serum cholesterol. Under the influence of Stimutacs normalization of the levels of total lipids and triglycerides. As is known, the biochemical composition of cell membranes reflects the cell metabolism in General, and therefore its functioning. In the study of phospholipid spectrum and lipid peroxidation of erythrocyte membranes in patients with psoriasis before treatment revealed an increase in the fraction of phosphatidylcholine (PC) and phosphatidylethanolamine (pea), as well as the decrease in the effective absorption with added LFH), indicating a violation of the permeability of erythrocyte membranes. After treatment with Climatecam there was a positive dynamics of these indicators. In the clinical study Stimutacs was recommended as a promising drug in the treatment of psoriasis, Stimutacs along with a high efficiency has good tolerability and safety.
Clinical testing of Stimutacs in the urology Department of the hospital. N. A. Semashko was preceded by the testing of patients by R. Voll.
Examination of patients and testing of the drug was carried out on hardware - software complex "IMEDIS-Voll" production company "IMEDIS". Testing was subjected to two dosage forms of the drug - suppositories and dried powder of Stimutacs. The method of testing was as follows: in the circuit device-PA is ient through the passive electrode is included, the test drug. Were used for testing BAHT (biologically active points) of those meridians that correspond to the intended pharmacological spectrum of action of the studied drugs.
By R. Voll surveyed and conducted medical testing 14 patients in four age groups:
30-40 years 3 persons;
40-50 years 3 persons;
50-60 years - 6 people;
60 years - 2 people.
Among patients identified 3 groups:
1. congestive and inflammatory diseases of prostate - 6 people,
2. prostate adenoma is 2 people,
3. without organic pathology to 6 people.
Studies were conducted on the following BATH:
1. The hypothalamus (evaluation of the effect of acute and chronic toxicity, psycho pathology and radicular cysts).
2. The pineal gland, the pituitary gland (indirect assessment antigonadotropic functions epiphysis and gonadotropic functions of the pituitary gland).
3. The sex glands, the adrenal glands.
4. The prostate gland, seminal tubercle, seminal vesicles, penis.
5. The epididymis, seminal cord.
Study above the BATH is allowed to determine the influence of drugs at all levels of the pathogenesis of the disease.
According to the test results it was possible to conclude that the effect of the tested dosage forms should be most pronounced at the following ur is vnah:
The hypothalamus, pituitary gland, sex glands, adrenal glands, prostate, epididymis.
Stimutacs in the tested formulations was effective in almost all age categories, but its effect was most pronounced in the age group of 50-60 years. According to the results of the conducted research it was possible to predict the next action of the investigational medicinal forms of drug:
1. Regulation of inflammatory and degenerative processes in the prostate gland.
2. Stimulation of gonadal function at the hypofunction.
3. Stimulation of spermatogenesis.
4. Braking congestive and inflammatory processes.
5. Resolving action on connective tissue, including benign tumors, scar changes and patterns.
6. improving hemodynamics genitals.
These studies served as the basis for conducting clinical trials of the drug in the group of patients with chronic prostatitis.
The group of patients consisted of 16 men aged 19 to 50 years old.
Stimutacs applied rectally in suppositories once a day for two weeks to one month.
The control group received standard therapy - Fipronil in suppositories.
Two patients with chronic prostatitis were combined with benign prostatic hyperplasia stage 1, the two pain who's was complicated urethritis. In 10 patients history moved STIs (chlamydia, ureaplasmosis, garderella), one patient removed the right kidney, chronic cystitis.
All the patients before treatment was examined: ultrasound of the prostate, the analysis of prostatic juice, swabs from the urethra. Before treatment and during treatment at the Department of functional diagnostics were conducted drug testing by R. Voll.
In the process of treatment in 15 of 16 patients reported a subjective and objective improvement. The positive dynamics was noted in the reduction or cessation of pain, the disappearance of a sense of burning in the urethra, reducing the frequency of urination, improve erection, increase sexual potency, the disappearance of pain during coitus. Compared with the control group, when use of the drug Stimutacs positive dynamics was noted in the earlier period - the fourth or fifth day of application instead of 10 days with standard therapy. Significantly reduced the terms of relief of symptoms of chronic cystitis. Persistent improvement occurred in the 14-30 day depending on the severity of the disease. A list of patients receiving Stimutacs.
|№ p/p||Surname ACTING sick||Agetd align="center"> no history of the disease|
|1||Klegin I.N.||50 years||107101|
|2||Pershin A.M.||37 years||106814|
|3||Kiselev DU||32||342 outpatient map|
|7||Nurutdinov AS||46 years||106949|
|9||Dubinin, J.V.||40 years||114044|
|13||Afanasiev S.V.||28 years||100661|
|14||Plovers Yu,P.||46 years||5071|
|15||Kitaev, VA||24||outpatient map|
|Martynov O.A.||27 years||11463|
All patients tolerated the drug well, allergies, complications and side effects have not been recorded.
As a result of testing Stimutacs recommended as a promising drug for the treatment of prostatitis.
Sources of information
1. Whisper, Humorously. Bulletin of the Academy of medical Sciences of the USSR, No. 6, 1990, p.19-27.
2. Medmaravis. Medicines, M., 1986, Vol.2.
3. A 61 K 9/14, 31/557 US 6251436 B1.
4. A 61 K 31/685 FR 2556050 A1.
5. A 61 K 35/12, 35/30, 35/48, 35/50 EN 2192871 C2.
6. Nperson. Metabolic aspects in dermatology, Sat. scient. works KVI, Bitter, 1984, pp.96-99.
Biologically active complex for the treatment of psoriasis and chronic prostatitis, characterized by the fact that he extracted from the testes of cattle sequential fractionation of carbon dioxide and ethyl alcohol, and includes phospholipids in the number of 56.5±4,0%free higher fatty acids and sterols in the number 17,2±2,5%, peptides and amino acids in the amount of 20.0±4,0%, as well as vitamins, macro - and microelements in the amount of 6.3%.
FIELD: medicine, dermatology, pharmacy.
SUBSTANCE: invention relates to a pharmaceutical gel composition for applying on skin. The composition comprises at least one vitamin D or analog of vitamin D, at least one corticosteroid, at least one solvent and an excipient enhancing viscosity. Viscosity value of the composition is in the range from 5 mPa x s to 500 mPa x s. The composition is designated for treatment of psoriasis and associated states in humans. The composition is suitable for applying on skin, shows the improved absorption and stable at 40°C in storage for 3 months.
EFFECT: improved and valuable properties of composition.
22 cl, 2 tbl, 1 ex
FIELD: organic chemistry, medicine, pharmacy.
SUBSTANCE: invention relates to derivative of triazaspiro[5.5]undecane of the formula (I): wherein R1 means compound of the formula (1): or (2): wherein G represents a bond, (C1-C4)-alkylene, (C2-C4)-alkenylene or -CO-; ring A represents: (1) C5-10-membered mono- or bicarbocyclic ring or (2) 5-10-membered mono- or bicyclic heterocycle comprising 1-2 nitrogen atoms and/or 1-2 oxygen atoms; substitute R6 means the following values: (1) (C1-C4)-alkyl, (2) halogen atom, (3) nitrile group, (4) trifluoromethyl group and others; R2 represents: (1) (C1-C4)-alkyl, (2) (C2-C4)alkynyl or (3) (C1-C4)-alkyl substituted with a substitute represented in claim 1 of the invention claim; each R3 and R4 represents independently: (1) hydrogen atom, (2) (C1-C4)-alkyl or (3) (C1-C4)-alkyl substituted with 1-2 substituted taken among: (a) Cyc 2 and (b) hydroxy-group (wherein Cyc 2 represents (1) C5-6-membered monocarbocyclic ring or (2) 5-6-membered monocyclic heterocycle comprising 1-2 nitrogen atoms and/or one oxygen atom), or R3 and R4 form in common group of the formula: wherein R26 represents (C1-C4)-alkyl or Cyc 2; R5 represents hydrogen atom or (C1-C4)-alkyl, its quaternary ammonium salt, its N-oxide or its nontoxic salt. Also, invention relates to pharmaceutical composition inhibiting HIV, regulator of chemokine/chemokine receptor and agent used in treatment and prophylaxis of some diseases, such as inflammatory diseases, asthma, atopic dermatitis, nettle rash, allergic diseases, nephritis, hepatitis, arthritis and other diseases that comprise as an active component above described compound of the formula (I) or its quaternary ammonium salt, its N-oxide or its nontoxic salt. Also, invention relates to (3R)-1-butyl-2,5-dioxo-3-((1R)-1-hydroxy-1-cyclohexylmethyl)-9-(4-(4-carboxyphenyloxy)phenylmethyl)-1,4,9-triazaspiro[5.5]undecane or its pharmaceutically acceptable salt and pharmaceutical composition based on thereof, and to (3R)-1-butyl-2,5-dioxo-3-((1R)-1-hydroxy-1-cyclohexylmethyl)-9-(4-(4-carboxyphenyloxy)phenylmethyl)-1,4,9-triazaspiro[5.5]undecane hydrochloride and pharmaceutical composition based on thereof.
EFFECT: valuable medicinal properties of derivative and composition.
16 cl, 32 ex
FIELD: organic chemistry, medicine, pharmacy.
SUBSTANCE: invention relates to R-2-aminoarylpropionic acid amides and pharmaceutical composition comprising thereof that can be used for prophylaxis and inhibition of recruiting and activation of leukocytes, and in treatment of pathologies directly dependent on indicated activation. Invention proposes compound of the general formula (1): wherein A, q, Ph and R have corresponding values, or its pharmaceutically acceptable salt. Also, invention describes a method for preparing amide of the formula (1) and pharmaceutical composition used in prophylaxis of leukocytes activation. Invention provides the development of pharmaceutical composition that can be used for prophylaxis and treatment of damaged tissues caused by enhancing activation of neutrophile granulocytes (polymorphonuclear leukocytes) in inflammation foci. Also, the invention relates to R-enantiomers 2-(aminoaryl)-propionylamides of the formula (1) that can be used for suppression of neutrophyles hemotaxis caused by IL-8. Also, compounds of this invention can be sued in treatment of psoriasis, ulcerous colitis, glomerulonephritis, acute respiratory insufficiency and rheumatic arthritis.
EFFECT: valuable medicinal properties of compounds.
8 cl, 16 ex
SUBSTANCE: method involves administering diacerine influencing DNA synthesis for reducing keratinocytes proliferation without changing their vital activity, inhibiting interleukines IL-1,IL-6 and α-TNF (tumor necrosis factor).
EFFECT: enhanced effectiveness of treatment.
11 cl, 7 dwg, 2 tbl
FIELD: organic chemistry, medicine, pharmacy.
SUBSTANCE: invention relates to new derivatives of triazaspiro[5,5]undecane of the formula (I):
wherein values of radicals R1-R5 are given in the invention claim, ort o their quaternary ammonium salts, N-oxides or nontoxic salts. Proposed compounds possess inhibitory and regulating activity with respect to chemokine/chemokine receptors and can be useful in prophylaxis and treatment of different inflammatory diseases, such as asthma, atopic dermatitis, nettle rash, allergic diseases, nephritis, hepatitis, arthritis or proliferative arthritis and other similar diseases. Also, invention relates to pharmaceutical compositions based on compounds of the formula (I).
EFFECT: improved control method, valuable medicinal properties of compounds.
9 cl, 5 sch, 36 tbl, 70 ex
FIELD: organic chemistry, pharmacy, biochemistry.
SUBSTANCE: invention relates to new substituted 2H-pyrano[2,3-c] of the general formula (1) eliciting ability to inhibit activity of protein kinase. In the general formula (1) X represents oxygen atom or group NR3; R1 represents group -C(O)R4, optionally substituted and optionally condensed azaheterocycle; R2 represents optionally substituted hydroxyl group or optionally substituted amino-group; R3 represents hydrogen atom or inert substitute meaning optionally substituted low- or non-reactive radical including such as (C1-C7)-alkyl, (C2-C7)-alkenyl, (C2-C7)-alkynyl, (C1-C7)-alkoxy-group, (C7-C12)-aralkyl, heterocyclylalkyl, (C7-C12)-alkaryl, (C3-C10)-cycloalkyl, (C3-C10)-cycloalkenyl, phenyl, aryl, (C2-C12)-alkoxyalkyl, (C2-C10)-alkylsulfinyl, (C2-C10)-alkylsulfonyl, -(CH2)-O-(C1-C7-alkyl), -(CH2)m-N(C1-C7-alkyl)n, aryloxyalkyl, heterocyclyl wherein m and n have value from 1 to 7; R4 represents optionally substituted amino-group or hydrogenated optionally substituted azaheterocycle. Also, invention relates to combinatory and focused libraries consisting of compounds of the present invention and designated for the search of compound-hits and compound-leaders prepared by screening of these libraries for using in preparing medicinal agents.
EFFECT: valuable medicinal properties of compounds.
8 cl, 2 tbl, 6 ex
FIELD: organic chemistry, vitamins, medicine, pharmacy.
SUBSTANCE: invention relates to a new compound of the formula (I): wherein X means hydrogen atom or hydroxy group; R1 and R2 that can be similar or different mean hydrogen atom, (C1-C4)-alkyl; R3 means hydrogen atom, methyl group, fluorine or chlorine atom. Also, invention relates to its esters able to hydrolysis in vivo in combination with pharmaceutically acceptable acids. Also, invention relates to a pharmaceutical composition eliciting the inhibitory activity with respect to proliferation and promoting differentiation of cells and comprising the effective dose of compound of the formula (I) in common with pharmaceutically acceptable carriers and/or excipients. Also, invention relates to applying compound of the formula (I) for preparing a medicine used in treatment and prophylaxis of disease characterizing by abnormal differentiation of cells and/or proliferation of cells.
EFFECT: valuable medicinal properties of compounds.
13 cl, 3 sch, 3 tbl, 6 ex
FIELD: organic chemistry, biochemistry, medicine, pharmacy.
SUBSTANCE: invention relates to new aminobenzophenones of the formula (I):
or their pharmaceutically acceptable salts. These compounds elicit properties of inhibitors of cytokines secretion, in particular, 1β-interleukin (IL-1β) and tumor necrosis α-factor (TNF-α) and to secretion of polymorphonuclear superoxide that are useful for treatment of inflammatory diseases, for example, skin diseases, such as psoriasis, atopic dermatitis. In the formula (I) R1 is taken among the group consisting of halogen atom, hydroxy-, mercapto-group, trifluoromethyl, amino-group, (C1-C3)-alkyl, (C2-C3)-olefinic group, (C1-C3)-alkoxy-, (C1-C3)-alkylthio-, (C1-C6)-alkylamino-group, (C1-C3)-alkoxycarbonyl, cyano-group, carbamoyl, phenyl or nitro-group under condition that when R1 means a single substitute then it at ortho-position, and when R1 means more one substitute then at least one substitute of R1 is at ortho-position; R2 means one substitute at ortho-position being indicated substitute is taken among the group consisting of (C1-C3)-alkyl, (C1-C3)-alkoxy-group; R3 means hydrogen, halogen atom, hydroxy-, mercapto-group, trifluoromethyl, amino-group, (C1-C3)-alkyl, (C2-C3)-olefinic group, (C1-C3)-alkoxy-, (C1-C3)-alkylthio-, (C1-C6)-alkylamino-group, (C1-C3)-alkoxycarbonyl, phenyl, cyano-, carboxy-group or carbamoyl; R4 means hydrogen atom or (C1-C3)-alkyl; Q means a bond or -SO2-; Y means (C1-C15)-alkyl, (C3-C10)-carbocyclic group or phenyl being each of them can be substituted optionally with one or some similar or different substitutes designated by the formula R5; R5 means halogen atom, (C1-C4)-alkyl, amino-, (C1-C3)-alkoxy-group, (C1-C3)-alkoxycarbonyl or -COOH; X means oxygen or sulfur atom. Also, invention relates to a pharmaceutical composition and to a method for treatment and/or prophylaxis of inflammatory diseases.
EFFECT: valuable medicinal properties of compounds and composition.
9 cl, 2 sch, 2 tbl, 29 ex
SUBSTANCE: method involves exposing the cyst in longitudinal direction under microscopic control with cyst cavity being filled with combined biological vascular neuro-fetoneural transplant. The transplant is blood vessel segment turned with its intima outside and filled with peripheral nerve fragments. Neutral or fetal embryo trunk cells are introduced into the central and peripheral transplant ends by means of injection after its being fixed. The region under plastic repair is additionally covered with greater omentum fragment on vascular pedicle.
EFFECT: enhanced effectiveness of recovery process; reduced cicatrix tissue formation in the injured region.
2 cl, 2 dwg
FIELD: medicine and biopharmacology.
SUBSTANCE: claimed biotransplant contains mesenchyme stem cells (MSC) obtained from fetal, donor or autologous material. Tissue is desagregated, obtained cell suspension is resuspended and cultivated on growth medium containing transferrin, insulin, fibroblast growth factor and heparin to accumulate mature stroma in cells. Method for treatment of lung hypertension includes intravenous drip-feeding of MSC in amount of 50-500 millions cells in 50-100 ml of physiological solution.
EFFECT: method for treatment of improved effectiveness having no undesired side effects.
FIELD: experimental medicine and oncology.
SUBSTANCE: claimed method includes intraperitoneum cyclophosphan administration in dose of 120 mg/kg and intravenous transplantation of fetal liver steam cells to experimental animal. Fetal liver steam cells are transplanted for 3 days before cyclophosphan administration in dose of 25x106 cells/kg of mass.
EFFECT: decreased tumor growth, reduced frequency and area of metastasis due to optimization of cyclophosphan and steam cells administration.
FIELD: medicine, gynecology.
SUBSTANCE: it is necessary to carry out combined medicinal and physiotherapeutic impact. Mixture of pharmacological preparations consisting of 80 mg gentamycine, 16 U lidase and 4 ml 2%-lidocaine solution should be injected submucosally into lateral vaginal arches followed by dynamic electroneurostimulation in area of uterine adnexa projection for 30 min. Therapy course lasts for 7 d. The innovation enables to shorten therapy terms and prevent the development of complications due to applying physiotherapeutic impact in acute period of purulent-inflammatory process.
EFFECT: higher efficiency of therapy.
SUBSTANCE: device has Dacron sack usable as envelope introducible into soft tissues of the head. The envelope has two 0.5 mm thick liver tissue leaflets between which hypothalamus tissue leaflets taken from young animal brain and black substance or isolated suprarenal gland medullary substance cells or that of human glands or the like cells taken from human embryo. The Dacron envelope is 25-30 cm long, 5 mm wide and 2 mm thick.
EFFECT: enhanced effectiveness of treatment.
2 cl, 1 dwg