Method for diagnosing neurocirculatory dystonia cases

FIELD: medicine.

SUBSTANCE: method involves determining lactate, pyruvic acid concentration, superoxide dismutase activity in patient saliva sample. Lactate concentration increase being equal to or and higher than 50%, pyruvic acid concentration drop being equal to or greater than 60% and superoxide dismutase activity being equal to or and higher than 50% when compared to norm, neurocirculatory dystonia diagnosis is to be set.

EFFECT: simplified noninvasive diagnosis method in preclinical stage cases.

 

The present invention relates to the field of medicine and will find application in the examination of children during mass medical examinations in order to predict the possible development of neurocirculatory dystonia in children.

Neurocirculatory dystonia (NDC) is the most common form of vegetariana in children and adolescents.

The actuality of it is that the prevalence of autonomic dysfunction in children and adolescents is significant and ranges from 20 to 30% of the population (N. Belokon, M.B., Koberger. "Heart disease and blood vessels in children. A guide for physicians", M.: Medicine, 1987, p.á305). At the same time, in the absence of timely diagnostic and therapeutic activities in a significant number of children NDC can acquire a strong character and be transformed in the future in hypertension, atherosclerosis, coronary heart disease.

Conducted research on the medical-scientific and patent literature identified various ways to diagnose NDC.

So, in the paper "characteristics of vegetative reactivity and autonomic ensure that children of school age" authors L.V. Kozlova, S. B. Kozlov and A.A. Yaylenko, medical school, Smolensk, published in the collection "the Age peculiarities of the physiological systems of children and adolescents",abstracts of the IV all-Union conference "Physiology of human development", M., 1990, s.138, provides a method for diagnosing the state of the autonomic nervous system according to the conduct of clinical and orthostatic tests (CPC). This Hyper sympathicotonic reactivity was detected in 36.7% of children asimpatikotonia - in 6.7%, indicating the exhaustion of the possibilities of sympathetic-adrenal system. The disadvantage of this method of diagnosis is the relative duration of implementation, and the need for the engagement of additional equipment (for recording kardiointervalogrammy).

In method I. Lazarev "Lipids and lipoproteins in the blood of patients with neurocirculatory dystonia cardiac type" (Diss. on saisc. degree KMN, M., 1986) provides that in children with different clinical types of NDC there is some relationship between the severity of the manifestation of clinical symptoms NDC and disorders of lipid and lipoprotein plasma dyslipidemia characterized by increased content of cholesterol in low density lipoprotein and a sharp increase in the level of triglycerides in the background of relatively constant total cholesterol, total lipids and atherogenic index. The disadvantage of this method of diagnosis NDC is the difficulty associated with blood sampling in children.

RF patent №2020870 B. I. No. 19 protected "Method of diagnostics is neurocirculatory dystonia", provide for the identification of preclinical stage of the disease by bringing the patient moving in cryptic rhythm of the visual stimulus. The disadvantage of this method is the duration of the study, the complexity of execution inaccuracy.

The closest technical solution chosen as a prototype, is "a Method for the diagnosis of dystonia autonomic nervous system in children with chronic gastroduodenal diseases (MD 2602 F1, publ. 30.11.2004). For implementing the method in children examine a sample of saliva, which photometrically determine the level of acetylcholinesterase and pseudocholinesterase, and, if the level of acetylcholinesterase is increased relative to norms, but pseudocholinesterase lowered, diagnosed with dystonia of the autonomic nervous system. The disadvantage of this method is the complexity of the method.

The aim of the present invention is to simplify the diagnosis of preclinical stage NDC for mass preventive examinations of children, increased availability, noninvasiveness.

This goal is achieved by examining the saliva of the child, which determine the level of lactate (lactic acid), pyruvic acid (PVA) and activity of superoxide dismutase (SOD), and increased levels of lactate by 50% or more compared to the norms of the th, reducing STC 60% or more, compared with the norm and the increase of SOD activity by more than 50% compared to the norm diagnosed with neurocirculatory dystonia.

The method is implemented as follows.

When conducting mass medical examinations of children, produce the sampling of saliva in the amount of approximately 1.0 ml, determine the level of lactate, the level of pyruvate and SOD activity. When detecting an increased level of lactate by 50% or more compared to the norm, reducing STC 60% or more, compared with the norm and the increase of SOD activity by more than 50% compared to the norm diagnose preclinical stage of the disease in children. The determination is carried out according to generally accepted standardized methods.

Examples of the proposed method.

Example 1. Child A., age 14, was in the children's hospital of Rostov state medical University (IB No. 1278/3) with complaints of headache, palpitations, fatigue, dizziness, sweating, shortness of breath. Preliminary examination did not fully carry out differential diagnostics between NDC and organic lesions of the CCC. Along with traditionally used blood, the child was taken saliva, which defined the level of lactate - 2.25 μmol/ml (increase of 56.9% compared to the norm), the level of STC - 0,40 çmol/ml (decrease by 60% compared to but the mine) and the activity of SOD 9,15 srvc. units/mg protein (an increase of 75.7% compared to the norm), which allowed to conclude that the child has a pronounced autonomic imbalance, on what basis was diagnosed with neurocirculatory dystonia.

Example 2. Child P., age 15, was on survey at the children's hospital of Rostov state medical University (IB No. 1264/2) with complaints of fatigue, inability to concentrate when doing homework, frequent headaches, dizziness, fainting, pain in the heart area. The study of saliva showed that the level of lactate was 2.02 mmol/ml, which was higher than in healthy children in the comparison group, 51.9%, the level of pyruvate was reduced and amounted to 0.14 mmol/l (below the norm on 85,9%), and SOD activity was increased to 9.80 srvc. units/mg protein (77.1% above normal). Diagnosed with neurocirculatory dystonia.

The proposed method is tested on two groups of children - healthy belonging to the 1st group health, and group of children on the examination of the pediatric Department of the Rostov state medical University clinic with a preliminary diagnosis: neurocirculatory dystonia. Each group included 40 children in different age categories. Researched saliva, to determine the level of lactate, STC, SOD activity. On the basis of indicators in the group of healthy children showed normal levels of lacto is a and STC and the activity of SOD, compared with the performance of STC, SOD, lactate in the second group and took them as a basis.

The proposed method is tested in the pediatric Department of the Rostov state medical University clinic and showed highly informative, accurate data, which clearly helped to improve the diagnosis NDC. The observation of such patients during the subsequent time showed that timely diagnosis NDC conducted preventive and curative measures prevented the transfer of functional changes in the organic.

Compared with the prototype of the proposed method enables faster, simpler, more reliable and, most importantly, non-invasive diagnostic neurocirculatory dystonia.

Method for the diagnosis of neurocirculatory dystonia in children, providing for the testing of saliva, characterized in that the saliva of the patient examine the level of lactate, the level of pyruvic acid (PVA), superoxide dismutase activity (SOD), and increased levels of lactate by 50% or more compared to the norm, reducing STC 60% or more, compared with the norm and the increase of SOD activity by more than 50% compared to the norm diagnosed with neurocirculatory dystonia.



 

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