Method for treating sepsis
SUBSTANCE: one should carry out antibacterial and infusion therapy. Moreover, additionally, after compensating the volume of circulating blood during infusion therapy one should intravenously inject 30 thousand IU antigangrenous polyvalent equine serum by drops per 200 ml isotonic NaCl solution: the first 1 ml solution should be injected for 5 min, the rest volume - for 1-1.5 h. The innovation suggested enables to optimize immunocorrecting therapy without sensitizing patient's body.
EFFECT: higher efficiency of therapy.
3 ex, 1 tbl
The invention relates to medicine, namely: intensive care, surgery, urology and gynecology.
Sepsis is a generalized inflammatory response of the host to excessive microbial load, caused not so much by the circulation in the blood of living organisms, how much excess blood gets of structural components and molecules of microbial origin from natural colonization (Navalmorales). Sepsis often called germs of the patient in the hospital (Vdelali).
Developed many effective treatments for sepsis: drainage foci localization and reproduction of microbes, antibiotic therapy in combination with aminoglycosides, detoxification methods through action on the main excretory organs, inhibitors of proteolysis (trental, gordox), respiratory support (humidified oxygen, mechanical ventilation), ultraviolet irradiation, immunostimulirutuyu therapy.
High mortality of patients with septic shock in the ICU, on average, 40% of the total mortality, (Vdelali)is the problem causing the search for new approaches to the treatment of sepsis. Extensive injuries. surgery, immunosuppressive therapy, unjustified antibiotic therapy, intoxication violate used, i.e. dynamic equilibrium Mick is Flory human and microorganism, violation of the colonization resistance of microbes. Abnormally proliferating in other ecological niches of microbes actively produce toxins. (Averoes). Even with a high level of reactivity of the microorganism and active phagocytosis intoxication, haemodynamic instability, each of these factors separately, especially in combination leads to translocation into the blood, the reticulo-endothelial system and connective tissue conditionally pathogenic microbes and their metabolites. In the treatment of this pathology is the drug "Pentaglobin".
"Pentaglobin" is intravenous immunoglobulin that binds endotoxin microbes.
"Pentaglobin" in high titer present antibodies to gram-negative bacteria. Early appointment of Pentaglobin, with gram-negative sepsis and septic shock, increases the survival of patients.
Dose Pentaglobin - 3-10 ml per kg per day, 3 days in a row/drip (causal and pathogenic therapy school, (Aromance, Lectures on topical issues of Pediatrics, 2002).
Contraindications to the drug "Pentaglobin":
1. You cannot enter with a high serum creatinine level, i.e. in renal failure.
2. The introduction of Pentaglobin can negatively affect the action of live vaccines against viral diseases.
Side effects, who may be on the introduction of Pentaglobin: chills, fever, headache, nausea, vomiting.
Documented cases of aseptic meningitis after the introduction of Pentaglobin.
As a prototype we have used a method of treating intestinal sepsis normal human immunoglobulin (application instructions approved by the Ministry of Health of the Russian Federation 23.04.02 year).
Normal human immunoglobulin is an immunologically active protein fraction, the active principle of which is the immunoglobulins of different specificity. The drug has also nonspecific activity, increasing the resistance of the organism.
Biological and immunological properties - neutralization of microbes and their toxins, antibodies contained in the immunoglobulin.
Route of administration: intramuscularly, intravenously. Maximum input single dose of 0.2 ml per kg of body weight, repeated administration through 24-72 hours.
The disadvantages of the prototype can be attributed to the fact that it is unstable to decomposition under the influence of bacterial protease, does not always produce the desired clinical result, the status of sepsis in patients may progress, it is necessary to use other means.
The essence of the proposed method as the invention is as follows.
As part of infusion therapy, the most severe, in the prognostic plan b is determined as being. it is proposed to use protivomigrenoznoe polyvalent equine serum - ASG.
Since 1967 CBC is used for emergency seroprevelance in military field conditions and traumatic injuries in the home in violation of the integrity of bone tissue. Application instructions approved by the head of the main Department of health care Ministry of health USSR 14.11.1988,
The main mechanism of action protivomigrenoznoe polyvalent equine serum is reduced to binding and neutralization of Exo - and endotoxins microbes antibodies contained in the serum.
Way. 30 thousand ME protivomigrenoznoe polyvalent equine serum is injected into/drip, 200 ml of isotonic NaCl after filling volume of circulating fluid. The first 1 ml for 5 min, the remaining capacity for 1-1,5 hours.
In our practice, ASG entered 23 surgical and 9 somatic patients with multiorgan failure. All patients except one surgery in satisfactory condition transferred to the relevant Department.
All patients who entered protivomigrenoznae polyvalent equine serum, in the next few days showed positive: improved reaction to external stimuli, normalized body temperature, while ately peripheral blood, biochemical parameters, patients were allowed paresis of the intestine.
Examples of specific implementation method.
1. Case history No. 2087.
26.02.04. Delivered patient with complaints: anxiety, bloating. fever, vomiting.
From the anamnesis: operated 16.02.04, Operation: dezinformatsia intestine. Diverticulectomy. A serious condition. The pale. Microcirculatory disorders. Toxic-exsicosis II Art. the probe from the stomach goes - stagnant stomach contents of green. In the tests: Hb - 88 g/l, L - 5,4·109, ESR - 55 mm/h
26.02.04, relaparotomy. Resection Powszechny intestine. Postoperative diagnosis: multiple perforations, diffuse purulent peritonitis, infectious gastroenterocolitis, infectious and toxic hepatitis. Toxic cordic.
In the analysis of increased t/aminase, (ALT - 1,4 AST - 1,6) Thymol turbidity test (1,6%) protein Total of 45.5 g/l In the analysis of peripheral blood lymphocytosis - 42, leukocytes - 13,2·109, ESR - 46 mm/h In the urine traces of protein, leukocyte count to 20 in sight bacteria.
Treatment was conducted. Respiratory support, antibiotics, selective decontaminate, cardiotonic, hepatoprotectors, perebrannoe introduction lidocaine, inhibitors of proteolysis, immunostimulirutuyu therapy (immunoglobulin - normal human 3 doses of 27, 28, 29 Feb the Ala). On the background of therapy continued intoxication. Tachycardia. Paresis of the intestine - probe departs from the stomach intestinal discharge. 02.03.04, with the composition of infusion therapy, on the 5th day operations transferred ASG/drip.
5.03.04, the State of moderate severity. Started enteral feeding. 10.03.04, the Patient is in satisfactory condition transferred to the relevant Department. A comparative analysis of the peripheral blood before and after the introduction of PACS is reflected in the table.
2. Case history No. 374.
Sick And born in 2001 (2 years 5 months) delivered in the ICU 11.01.03, in a very severe condition of CRH, Gudermes.
From the anamnesis: sore 5 days. The disease began with fever up to 38.5 C. anxiety. The patient, CRH, Gudermes, to suppress heat production, inside and externally appointed Apple essence (in a dilution of 2 teaspoons per Cup of water), aspirin1/2TB., ampicillin. Planning: menadione, nicotinic acid, calcium gluconate, hormones in the dose of 20 mg/kg / day (1295 mg). At the time of admission in the ICU patient in a coma I expressed dyspnea mixed body temperature - 38,8°, tachycardia, microcirculatory disorders, paresis of the intestine. On the radiograph - ocharovatelnye shadows. Liver and spleen were enlarged. No urine.
In the analysis of peripheral blood leukocytes 49,10, l, limonene (3-4 in p the Le view - the result of immunosuppression) the infusion of large doses of hormones. Coagulation on the Moravica 1 minute, 30 seconds, thrombocytopenia, anemia. In the biochemical analysis of blood - increased grantability (ALt, Ast - 1.2 mmol/l). Urea - 13 mmol/l In the urine are all leukocytes, protein. Locally lower extremity purple-bluish color on both limbs (R. 3×4 cm), bubbles with clear content.
Diagnosis: 11.01.03, Chemical burns of the oropharynx, esophagus, pneumonia, toxic hepatitis, toxic kordic, coma. Thrombosis of the lower extremities. Sepsis.
Against the backdrop of ongoing respiratory support (mechanical ventilation), antibiotic therapy, heparin therapy (500 IU/kg), after the restoration BCC, physiological solution introduced ASG 1 dose (30 thousand ME) 2 times with an interval of 24 hours. On day 3 after 2 injections improved reaction to external stimuli, normalized body temperature and peripheral blood, stabilized hemodynamics. Was sanitize foci of infection. In satisfactory condition of the patient is directed, in Moscow for autotransplantation.
3. Case history No. 11293/545.
17.10.2003. Patient a, age 1 year, 1 month delivered from the Central district hospital (n midwives) complaints: abdominal pain, vomiting, fever to 38.9°, bespokoit is O. On the day of treatment in the analysis of blood leukocytes of 19.2·109/l; Hb - 112 g/l; signs of systemic inflammation.
In the analysis of urine protein - 3.5 g/l erythrocytes, leukocytes entirely, bacteria +++.
17.10.2003, Surgery: laparotomy. Revision of the abdominal cavity. Pyelostomy left. Postoperative diagnosis: congenital hydronephrosis on the left on the basis of narrowing pyeloureteral segment. Conservative: Ampioks 500 mg 3 times a day; Reopoliglyukin; fresh Frozen plasma, 10% glucose, trental, analgesics.
23.10.2003 year - on the sixth day after the operation, the condition worsened; vomiting 3 times. Fever - 39°With tachycardia. HR - 168 beats per minute. Locally: belly swollen, tense, painful on palpation. Drainage from the abdominal cavity departs purulent discharge, in the field of wound infiltration. During inspection of the wound - dirty-hemorrhagic fluid.
23.10.2003, Operation: lumbotomy. Resection of the pelvic-ureteric segment. Pyeloureterostomy. Nephroscope. Postoperative diagnosis: congenital stenosis paleorecharge segment. Hydronephrosis. After laparotomy, parametric, urinary numb. Peritonitis.
25.10.2003, the Patient is pale, the temperature of 37.8°C. Vomiting, persistent microcirculatory disorders. Locally: abdominal pain, the phenomenon of paresis of the intestine, the probe of the stomach stagnant detachable.>
In the analysis of urine protein 0.3 g/l, leucocytes, erythrocytes. Visually: the urine is concentrated, mixed with erythrocytes. UD. the weight of urine 1023. Patient 25.10.2003, intravenous, drip ASG. 1 dose (30000 thousand IU).
27.10.2003 normalized body temperature, stabilized hemodynamic parameters, has allowed paresis of the intestine. The patient is in satisfactory condition transferred to the relevant Department.
When using CBC with curative intent, in the complex treatment of patients with sepsis positive results in 30 patients in the absence of side effects in the coming days after injection.
Signs, distinctive features of the prototype:
in the treatment of intestinal sepsis this drug was not used.
The usefulness of the proposed method is as follows:
- to achieve a stable, fast, a positive result from one or 2 injections ASG, already on the 2nd, 3rd day observed clinical and laboratory results;
- optimization of immune-correcting therapy without sensitization of the patient;
- shorter hospital stay heavy patients;
- the absence of contraindications to the drug;
- resistance to cleavage by bacterial toxins and enzymes.
|Show is whether peripheral blood and t - reaction before and after the introduction of PACS|
|Days after surgery||2||3||4||5||6||7||8|
A method of treatment of sepsis caused by intestinal infection, which consists in the carrying out of antibacterial therapy, characterized in that in the treatment of sepsis in the composition of infusion therapy, the patient is injected 30 thousand IU protivomigrenoznoe polivalent the Noah horse serum intravenously in 200 ml of isotonic NaCl after filling volume of circulating fluid, the first 1 ml injected for 5 min, the remaining capacity for 1-1,5 hours
FIELD: pharmaceutical industry, in particular drug containing human recombinant alpha-2 interferon.
SUBSTANCE: claimed prolonged solution contains human recombinant alpha-2 interferon, citric acid, boric acid, sodium tetraborate, unithiol, human serum albumin, sodium chloride, sodium carboxymethylcellulose and purified water. Preparation of present invention has wide spectrum of therapeutic application.
EFFECT: drug of high specific antiviral and antimicrobial activity without side effects.
1 tbl, 3 ex
FIELD: medicine, pediatrics.
SUBSTANCE: in small children at syndrome of increased thymus gland degree 1-3 one should prescribe prednisolone and eubiotics simultaneously, and at degree 2-3 it is necessary to prescribe immunomodulating agents, additionally. The innovation provides increased anti-infectious immunity and inspecific body resistance due to efficient correction of hormonal state in this group of children.
EFFECT: higher efficiency of prophylaxis.
2 cl, 1 ex
FIELD: veterinary obstetrics.
SUBSTANCE: the method deals with subcutaneous injection of bioglobin (placenta denaturated suspended - PDS) at the quantity of 20 mg/animal/d not earlier that 10 d before calving. The innovation enables to increase quality in preventing the onset of functional dyspepsia in newborn calves and level of total body resistance.
EFFECT: higher efficiency.
20 dwg, 1 ex, 2 tbl
SUBSTANCE: the present innovation deals with treating vestibular and auditory disorders, trigeminal nerve's neuralgia and peripheral paresis of facial nerve of herpetic etiology. The method deals with introduction of an antiherpetic preparation followed by immunomodelling therapy with the use of polyoxidonium. Moreover, after treating with antiherpetic preparation before introducing polyoxidonium it is necessary to conduct additional successive therapy: with preparations of trophic action as milgamma or neuromultivit at simultaneous introduction of antioxidant, then comes intratissue injection of cerebrolysine being behind the top of mastoid process.
EFFECT: the innovation enables to decrease the quantity of relapses due to stopping the development of herpes simplex virus, restore conductivity along nervous fiber and improve endoneural circulation.
3 cl, 3 ex
FIELD: medicine, chemical-pharmaceutical industry, pharmacy.
SUBSTANCE: invention relates to using 4-chloro-2-methylphenoxyacetic acid of the formula (I)
and its pharmacologically acceptable sodium, potassium and lithium salts (mixture of these salts, or mixture of salts and 4-chloro-2-methylphenoxyacetic acid) as a medicinal agent possessing immunomodulating, anti-inflammatory and antitumor properties, and antiviral activity also. 4-Chloro-2-methylphenoxyacetic acid and its mixtures with pharmacologically acceptable alkaline metal salts possess high effectiveness and enhanced bioavailability.
EFFECT: valuable medicinal properties of medicinal agent.
9 cl, 13 ex
FIELD: chemistry of peptides, medicine.
SUBSTANCE: invention relates to preparing new peptides possessing immunomodulating, anti-proliferative, anti-tumor and antiviral activity. Invention proposes new peptides comprising up to 30 amino acid residues of the general structural formula: X1-Trp-Gly-Gln-X2 wherein X1 is taken among the following group: -His-Gly-Val-Ser-Gly-, -His-Gly-Gly-Gly-, -His-Val-Gly-Gly-, -His-Gly-Gly-Gly-Gly-, and -Gln-Gly-Gly-Gly-Gly, or absent; X2 is taken among the following group: -His-Gly-Thr-His-Gly, -Gly-Gly-Thr-His-Gly, -Pro-His-Val-Gly-Gly, -Pro-His-Gly-Gly-Gly, -Pro-His-Gly-Gly-Gly-Trp-Gly, -Gly-Gly-Gly-Thr-His-Ser, or absent.
EFFECT: valuable medicinal properties of peptides.
8 cl, 5 tbl, 5 dwg, 6 ex
SUBSTANCE: method involves administering diacerine influencing DNA synthesis for reducing keratinocytes proliferation without changing their vital activity, inhibiting interleukines IL-1,IL-6 and α-TNF (tumor necrosis factor).
EFFECT: enhanced effectiveness of treatment.
11 cl, 7 dwg, 2 tbl
FIELD: medicine, chemistry of peptides, amino acids.
SUBSTANCE: invention relates to novel biologically active substances. Invention proposes the novel composition comprising peptides of the formula: H-Arg-Gly-Asp-OH and H-Tyr-X-Y-Glu-OH wherein X means Gln and/or Glu; Y means Cys(acm) and/or Cys. The composition shows ability to inhibit proliferative activity of mononuclear cells, to induce suppressive activity and their ability for secretion of cytokines TNF-1β (tumor necrosis factor-1β) and IL-10 (interleukin-10 ).
EFFECT: simplified method for preparing composition, valuable medicinal properties of composition.
4 cl, 16 tbl, 9 ex
FIELD: medicine, phthisiology, pharmacy.
SUBSTANCE: invention proposes the inhalation preparation in treatment of tuberculosis comprising an antibacterial medicinal agent an aqueous solution. The preparation comprises also a medicinal preparation with the immunomodulating effect as 5-amino-2,3-dihydro-1,4-phthalazinedione potassium salt or 5-amino-2,3-dihydro-1,4-phthalazinedione sodium salt, or 5-amino-2,3-dihydro-1,4-phthalazinedione potassium and sodium salts taken in the equal ratio in the following ratio of components, wt.-%: antibacterial medicinal agent, 1.0-10.0; 5-amino-2,3-dihydro-1,4-phthalazinedione potassium salt or 5-amino-2,3-dihydro-1,4-phthalazinedione sodium salt, or 5-amino-2,3-dihydro-1,4-phthalazinedione potassium and sodium salts taken in the equal ratio, 1.0-10.0; water, the balance. The preparation inhibits activity of macrophages reducing the level of tumor necrosis factor (TNF) and acute-phase proteins that results to weakening intoxication symptoms, activates superoxide-forming function and phagocyte activity of neutrophile granulocytes, enhances the microbiocide system of cells that, in turn, results to arresting the inflammation process. Invention can be used as the inhalation agent in treatment of tuberculosis.
EFFECT: valuable medicinal properties of preparation.
2 cl, 2 ex
FIELD: medicine, rheumatology, pharmacy.
SUBSTANCE: invention proposes ointment as a curative agent that comprises the following components: 5-amino-2,3-dihydro-1,4-phthalazinedione alkaline salt of mixture of alkaline salts, medicinal vaseline, vaseline oil as an ointment base and distilled water taken in the following ratio of components, wt.-%: 5-amino-2,3-dihydro-1,4-phthalazinedione alkaline salt or 5-amino-2,3-dihydro-1,4-phthalazinedione mixture of alkaline salts, 1.0-50.0; medicinal vaseline, 10.0-20.0; vaseline oil, 5.0-10.0, and water, the balance, wherein 5-amino-2,3-dihydro-1,4-phthalazinedione sodium or potassium salt is used as an alkaline salt, and 5-amino-2,3-dihydro-1,4-phthalazinedione sodium and potassium salts mixture is used as mixture of alkaline salts composed of the equal ratio of components, i. e. 5-amino-2,3-dihydro-1,4-phthalazinedione sodium and potassium salts mixture. Indicated ointment normalizes membrane cell to the normal state damaged as result of disease, regulated intra- and intercellular metabolic processes that, in turn, promotes to normalization of metabolism and arresting inflammation for the shortest times. Invention can be used in treatment of autoimmune disease.
EFFECT: improved and valuable medicinal properties of ointment.
SUBSTANCE: invention relates to immunoenzyme analysis and can be used for assay of von Willebrand factor. Method involves immunoenzyme analysis wherein monoclonal antibody 5C3 is used as an immobilizing antibody, and a mixture of biotin-labeled monoclonal antibodies 2H2 and 7D12 is used as a detecting antibody. Also, invention relates to monoclonal antibodies produced by the strain of hybridoma cultured cells Mus musculus L. and directed against von Willebrand factor, and to strains of hybrid cultured cells Mus musculus L. producing indicated monoclonal antibodies. Invention provides the development of highly sensitive method for assay of von Willebrand factor.
EFFECT: improved method for analysis.
9 cl, 1 tbl, 2 dwg, 3 ex
SUBSTANCE: obtained human antibody or its antigen-binding fragment specifically binds tumor necrosis factor hTNFα. The like antibodies show high affinity relative to hTNFα in vitro and in vivo. Antibodies according to the invention are taken as a full-length antibody or its antigen-binding fragment. The antibodies or their fragments are usable for detecting hTNFα and for inhibiting hTNFα activity in human beings suffering from a disorder in the case of which hTNFα activity is harmful.
EFFECT: wide range of applications of high affinity recombinant antibodies to hTNFα or their fragments of low dissociation kinetics.
15 cl, 11 dwg, 17 tbl
FIELD: medicine, oncology, immunology.
SUBSTANCE: invention relates to humanized antibodies with ErbB2. Invention involves the development of new humanized antibodies raised to tyrosinase receptors of family ErbB2, and to a composition comprising these antibodies. The advantage of invention involves expanding region in using indicated antibodies in cancer treatment wherein receptor of epidermal growth factor, EGFR, is a target of these antibodies.
EFFECT: valuable properties of antibody.
14 cl, 3 tbl, 13 dwg
SUBSTANCE: the present innovation deals with increasing life duration period due to deteriorating the onset of aging-associated sick states. For this purpose, it is necessary to introduce an agent that inactivates extra-cellular blood DNA into blood circulation. AS such an agent one should apply DNAse, antibody anti-DNA, enzymes that alter chemical structure of extra-cellular blood DNA, and, also, agents that stimulate synthesis or activity of endogenous DNAse or that of antibodies that bind extra-cellular blood DNA. The method provides high therapeutic effect in case of no direct impact upon genetic apparatus of aging cells.
EFFECT: higher efficiency.
6 cl, 8 ex, 4 tbl
FIELD: biotechnology, immunology, molecular biology, medicine, pharmacy.
SUBSTANCE: invention describes the isolated human antibody or its antigen-binding fragment able to bind the human tumor necrosis factor (TNF-α). Amino acid sequence is given in the description. Invention discloses nucleic acid encoding heavy and light chain of isolated human antibody. Nucleotide sequences are given in the description. Invention describes recombinant vector expressing variable region of heavy and light chains of isolated human antibody, Chinese hamster ovary cells CHO dhfr- carrying vector. Invention discloses a method for synthesis of isolated human antibody. The isolated human antibody or its antigen-binding fragment can be used as an active component of pharmaceutical composition used in treatment of disturbances when activity of TNF-α is harmful. Using the invention allows neutralization of effect of TNF-α in case when its activity is harmful. Invention can be used in medicine.
EFFECT: valuable medicinal properties of antibody, improved method for synthesis.
17 cl, 11 dwg, 17 tbl, 4 ex
SUBSTANCE: binding structure is to be bound in tumor cells and/or to tumor cell surface. Target structure available and/or expressing in the tumor cells and/or on tumor cell surface. The binding structure recognizes and blocks the target structure. Substance binding to the target structure or blocks the target structure expression is described. Pharmaceutical compositions comprise the binding structure, target structure or the substance as active principle. Methods for making phage selection, and methods for making in-vitro and in vivo diagnosis and prognosis and methods for treating malignant human diseases provide for the materials usage.
EFFECT: enhanced effectiveness in treating tumor diseases.
42 cl, 14 dwg, 4 tbl
FIELD: medicine, veterinary science.
SUBSTANCE: the present innovation deals with treating malignant tumors, or infections, caused by bacteria, fungi and protozoa, or atherosclerosis, or diabetes mellitus, or diseases associated with delayed-type reaction of hypersensitivity, or diseases developed due to mutations of somatic cells' genes. For this purpose, one should introduce an agent that binds blood extra-cellular DNA, or an enzyme to alter chemical structure of blood extra-cellular DNA, or blood should be supplemented with an agent that stimulates either the synthesis or activity of endogenous desoxyribonuclease or an agent that stimulates the synthesis of antibodies that bind blood extra-cellular DNA. The innovation suggested enables to obtain high efficiency of low-toxic etiological treatment of the diseases mentioned above.
EFFECT: higher efficiency of therapy.
3 cl, 3 dwg, 14 ex, 10 tbl
FIELD: medicine, oncology, gastroenterology, immunobiotechnology.
SUBSTANCE: invention describes an antibody or its derivative, or its fragment showing the structure able to bind the target structure. Antibody is located inside and on surface of human gastroenteric tract epithelial tumor cells and in subpopulation of normal gastroenteric tract epithelial cells. Indicated binding structures comprise sequences determining the complementarity of the region (CDR) in light chain comprising in main amino acids at number 23-33 (CDR 1), 49-55 (CDR 2), 88-98 (CDR 3) of amino acid sequence represented in SEQ ID NO:2, and CDR sequence in heavy chains comprising in main amino acids at number 158-162 (CDR 1), 177-193 (CDR 2), 226-238 (CDR 3) of amino acid sequence represented in SEQ ID NO:2, or other binding structures with similar unique binding properties. Also, invention describes the target-structure located inside or on surface of tumor cells: vaccine composition designated for treatment of malignant disease in human and comprising abovementioned antibody. Also, invention describes methods for treatment and diagnosis of malignant disease. Using this invention provides preparing antibodies that relieve identification of new phenotype-specific tumor-associated antigens, to predict and treat metastatic human diseases. Invention can be used in medicinal practice.
EFFECT: valuable medicinal properties of antibodies.
37 cl, 21 dwg, 4 tbl, 8 ex
SUBSTANCE: the present innovation deals with immunobiological medicinal preparations applied for intravenous injection at correcting immunodeficient states of different genesis. One should immunize animals with human thymus gland's cells, isolation of immune plasma, its depletion with plasmatic proteins AB (IV) human blood group followed by plasmatic fractioning with PEG 6000 and 20000, depleting semi-product with cellular components of the mixture of all four human blood groups and purification of the target product with PEG 6000. As a stabilizing agent on should apply glycine.
EFFECT: more simplified method.
4 cl, 3 ex
FIELD: immunology; treatment of mediated diseases IL-1 and failures.
SUBSTANCE: bonding molecule IL-1β which is antibody to human IL-1β and especially human antibody to human IL-1β where hypervariable sections CDRs of heavy and light chains have definite amino acid sequences. Antibody may be used for treatment of mediated disease IL-1, for example osteoarthritis, osteoporosis and other inflammatory processes of bones of rheumatism or podagra nature. Constructions of deoxyribonucleic acid are described which code heavy and light chains or their fragments and expressive vectors which may be replicated in cells including deoxyribonucleic acid constructions. Method of obtaining bonding molecule IL-1β by means of cell transformed by vector is described. Proposed antibody may be used both in prophylactic and treatment of diseases.
EFFECT: enhanced efficiency.
15 cl, 3 dwg, 5 ex
FIELD: genetic engineering, immunology, medicine.
SUBSTANCE: invention relates to new antibodies directed against antigenic complex CD3 and can be used in therapeutic aims. Antibody IgG elicits the affinity binding with respect to antigenic complex CD3 wherein heavy chain comprises skeleton of the human variable region in common with at least one CD3 taken among amino acid sequences SEQ ID NO 2, 4 and 6 and their corresponding conservatively modified variants. Light chain comprises skeleton of the rodent variable region in common with at least one CD3 taken among amino acid sequences SEQ ID NO 8, 10 and 12 and their corresponding conservatively modified variants. Antibody is prepared by culturing procaryotic or eucaryotic cell co-transformed with vector comprising recombinant nucleic acid that encodes antibody light chain and vector comprising recombinant nucleic acid that encodes antibody heavy chain. Antibody is administrated in the patient suffering with malignant tumor or needing in immunosuppression in the effective dose. Invention provides preparing chimeric antibodies against CD3 that are produced by expression systems of procaryotic and eucaryotic cells with the enhanced yield.
EFFECT: improved preparing methods, valuable medicinal properties of antibody.
33 cl, 5 dwg, 1 ex