Method for evaluating compensatory manifestation at affecting articular components in animals under conditions of modeling destructive-dystrophic process

FIELD: veterinary medicine.

SUBSTANCE: one should twice detect glycosaminoglycanes refraction coefficient: before and after compensatory impact and according to special formula it is possible to detect diagnostic criterion - coefficient of compensatory manifestation (CCM): where RC1 - glycosaminoglycanes refraction coefficient before impact, RC2 - glycosaminoglycanes refraction coefficient after compensatory impact, at CCM value being below 1.5 one should provide negative evaluation for compensatory manifestation, and at CCM value being 1.5 and more compensatory manifestation should be considered to be adequate. The innovation enables to unify evaluation of the impact performed upon articular components in animals.

EFFECT: higher accuracy of evaluation.

49 dwg, 1 tbl

 

The present invention relates to veterinary medicine and can be used in normal and pathological anatomy, as well as in orthopedics, traumatology and prosthetics in the operational period regarding the condition of the musculoskeletal system.

A known method for predicting outcomes in mechanical trauma [SU 1782547 A1, 5A 61 B 10/00, 23.12.1992], the essence of which consists in the measurement of systemic and diastolic arterial blood pressure, calculating a prognostic index (PI):where the sad and dad systolic and diastolic blood pressure, mm RT. Art., and predicting outcomes of traumatic disease: when PI is equal 0,86 and less predicted a favorable outcome, but values PI of 0.87 and a more adverse outcome. However, this method does not reflect the morphological pattern that is emerging in the area of injury as such in General and in the joint in particular, and does not take into account the changing structures of the joint components.

Closest to the claimed is cytological analysis method chondrocytes [Annefeld M. Veranderungen in der Ultrastructur der Chondrocyten unter dem Einflub a GAG-Peptid-Komplex / M.Annefeld, R.Raiss // Aktuelle Rheumatologie. - 1984. - S.2, Bd.9. - S.99-105.], the essence of which is to use parameters reflecting ultrastructural organization of chondrocytes to determine the degree of degenerative-dystrophic is oragene joint. However, this cytological analysis method chondrocytes requires high qualification and time-consuming.

The task of the invention is to reduce the time to conduct research and increase its accuracy, the unification of the impact assessment components of the joints of animals.

The essence of the invention lies in the fact that determine not only the integrity of the structural zones of cartilage, but the orientational order of molecules in the intercellular substance and its heterogeneity as an indicator of completion of interstitial processes of differentiation on the basis of the refractive index of collagen, the degree of morphological maturity and orientational order.

The method is as follows. Histological sections stained by the dye molecules are dipoles, and passed through them linearly polarized light, what causes the effect of optical anisotropy and study the orientational order of the major macromolecules of the matrix of the articular cartilage and ligaments in the polarized light microscope "Polmy" (Poland) by quantification (using the compensator λ/4) values of refraction (double refraction) of macromolecules identified in histological reaction is; for collagen - phenol, picrosirius red, for glycosaminoglycans with toluidine blue (pH 1,5; 2,5; 3,5) before and after compensatory way, and the formula to determine the diagnostic criterion is the ratio of the manifestations of compensation (CCP):where CU1the refractive index of glycosaminoglycans before exposure, CU2the refractive index after exposure, when the value of the PDA less than 1.5 give a negative assessment of the manifestations of compensation, and if the value PDA 1.5 and more manifestation of compensation is adequate.

Example. The proposed method was reproduced in a series of experiments: the first - intact animals (dogs); second, the modeling of degenerative-dystrophic lesions injected into the knee joint of prednisolone at a dose of 15-25 ml 1-2 times in two weeks during the month; the third simulation degenerative-dystrophic lesions injected into the knee joint of prednisolone at a dose of 15-25 ml 1-2 times in two weeks in the month, the introduction of buffer; the fourth is the modeling of degenerative-dystrophic lesions injected into the knee joint of prednisolone at a dose of 15-25 ml 1-2 times in two weeks in the month + of glucosamine hydrochloride in a dose of 15-25 mg/kg intramuscularly, 1-2 times per week during the month. Animals were taken from experiment by meredosia and thiopental sodium in compliance with the rules of work with the use of experimental animals and the requirements of the Ministry of Health of the Union of Soviet Socialist Republics No. 12000-496 on April 2, 1980 Monitoring the condition of the joints consisted of a complex of methods, including thin anatomical dissection followed by a description and functional analysis of their structures.

Micromorphological research is subjected to the fresh samples of articular cartilage and subchondral bone condyles of the femur and lateral ligaments from the knee joint from 32 dogs. The study of General morphological patterns in the light microscope (Jenamed-2, Karl-C, Jena) is carried out after staining the sections with hematoxylin and eosin, funcionam according to HART for evaluation of elastic fibers. The latter structures were carried out under a microscope MBI-6" (eyepiece (OK) 7, 10, lens (about.) 8, 10, 20) using an eyepiece micrometer 1*16. The study of the orientational ordering of the major macromolecules of the matrix is performed in polarized light microscope "Polmy-A" (Poland) by quantification (using the compensator λ/4 Senarmont) values of refraction of macromolecules identified in histological reactions: for collagen - phenol and picrosirius red with toluidine blue for glycosaminoglycans at pH 1,5; 2,5; 3,5.

The structure of the joint components in terms of modeling the destructive process by intraarticular injection of prednisolone. As a result of the research showed that intra-articular injections prednis the womb is called the knee joint complex morphological changes destructive Genesis, manifested both at the macroscopic and microscopic levels of organization. So, after a month after intra-articular injection of prednisolone marked stiffness of the knee joint, which increased in volume and swollen. Animals prefer not to include it in locomotor act. The joint capsule is hyperemic and edematous, in the area of attachment to the bone it identified areas of dark color, defined by us as necrotic foci. The detected intra-articular education with signs of petechiae and Akimoto, which is possibly a consequence of diapedesis.

Articular cartilage covering Misaki, uneven thickness, in places scarification to exposure of subchondral bone. Cartilage coating loses its luster, has a matte finish and is characterized by changing the natural color from blue-gray to brown. The area of the defect is made regenerate white, the structural design of which resembles elastic connective tissue. When analyzing historiarum damaged condyles shown that destructive transformations affect all components of the proximal metapelite: articular cartilage, subchondral bone and metatithemi cartilage. So, articular cartilage variable in thickness, with foci of atrial, cracks and slots of various configurations and lengths. Neobhodimosti be noted, revealed microdefects can have a longitudinal direction (Fig 1. Microarchitecture articular cartilage in terms of modeling extreme impact. About. 10, OK. 8. Usource cartilage, tangential and transverse cracks, razvlechenie cartilage). Razvlechenie cartilage predominantly affects its intermediate zone, while for the surface zone is characterized usource cartilage surface and the violation of its topography (figure 2. Microarchitecture articular cartilage in terms of modeling extreme impact. About. 10, OK. 8. Usource cartilage, tangential and transverse cracks, razvlechenie cartilage). In the area of cracks and razvelecheny draws attention to the violation of cytoarchitectonic cartilage tissue that is manifested in the reduction of the quantitative representation of chondrocytes and the appearance of their destructive forms (figure 3. Destruction of the articular cartilage after intra-articular injection of prednisolone. Hematoxylin and eosin. About. 10, OK. 8). In the area of damage to the articular surface it is easy to see the violation of the integrity of basophilic section, which can lead to changes in the mineralization of the cartilage matrix (figure 4. Violation of the integrity of basophilic section and usource cartilage after intra-articular injection of prednisolone. Hematoxylin and eosin. About. 10, OK. 8). In addition, in the calcified zone of graseful the cover marked invasion of blood vessels, the consequence is the appearance of hypertrophic chondrocytes, which is in the state of picknose and necrosis, and the replacement of cartilage tissue coarse-fibered bone (figure 5. Invasion of blood vessels in the deep zone of cartilage in experimental modeling of extreme impact. Hematoxylin and eosin. About. 10, about 8, 6. Invasion of blood vessels in the deep zone of cartilage in experimental modeling of extreme impact. Hematoxylin and eosin. About. 10, OK. 8).

In parts of the cartilage adjacent to the damaged identified reclaim represented by the chondrocytes, which are in the form of ribbon-like accumulations in the calcified zone and fibrous formations, localized in the surface area (Fig.7. Fibrous regenerate in the surface area of damaged cartilage. Modeling of extreme impact. Hematoxylin and eosin. About. 10, OK. 8, Fig. Fibrous regenerate in the surface area of damaged cartilage. Modeling of extreme impact. Hematoxylin and eosin. About. 10, OK. 8, figure 9. Androidmy proliferate in the deep zones of articular cartilage in the simulation of intra-articular destructive process by extreme exposure. Hematoxylin and eosin. About. 10, OK. 8, figure 10. Androidmy proliferate in the deep zones of articular cartilage in the simulation of intra-articular destructive process by e Strekalova impact. Hematoxylin and eosin. About. 10, OK. 8).

Polarization-optical analysis of drugs has convincingly demonstrated that in the area of damage to articular cartilage is disturbed orientation of the collagen structures of cartilage matrix, which is expressed in their tangential location in a deep divisions, whereas in intact animals they have a vertical orientation of the free surface (11. The architectonics of the collagen fibers in the damaged articular cartilage. Phenol reaction (reaction Ebner). About. 10, OK. 8). In the intermediate zone of the cartilage, there is no phenomenon of refraction that may be associated with its depletion of collagen fibers and weakening of metabolic potentials. At colouring preparations toluidine blue found that the damaged cartilage, performed by the regenerate, changes its tinctorial properties (does not have metachromasia), which may reflect redistribution of glycosaminoglycans and bound water, which, as is well known, determines the resistance of cartilage to power loads in compression (Fig. The distribution of glycosaminoglycans in the field of damage to the articular cartilage. Toluidine blue. About. 10, OK. 8). This feature in the structural organization of damaged areas can testify to their insolvency in biomechanical terms. In the area of damage to the articular cartilage showing the but the prevalence of sulfated forms of glycosaminoglycans, giving γ-metachromasia. This fact may indicate high biosynthesise activity of these areas, in comparison with adjacent areas, in which prevail vysokoerudirovannye forms of these proteoglycans (Fig. Representation of glycosaminoglycans in damaged and allied departments of the articular cartilage in experimental modeling of extreme impact. Toluidine blue. About. 10, OK. 8, Fig. Representation of glycosaminoglycans in damaged and allied departments of the articular cartilage in experimental modeling of extreme impact. Toluidine blue. About. 10, OK. 8). It is not excluded that the described changes in the content of the carbohydrate-protein complexes are adaptive in nature. There is reason to believe that the source of the regenerate cartilage can serve as the subchondral bone in its Central departments and the joint capsule in the peripheral zones. However, the new generation of cartilage do not have time to reach that degree of morphological maturity, which is characteristic of the cartilage covering the intact animals that may be associated with the additional trauma of a young androidney tissue structural organization which is not adequate to the incident power loads.

Structural changes to the subchondral bone are in violation of osteogenesis. Uh what about the expressed thinning and resorption of bone trabeculae with the formation of resorption lacunae, filled with detritus, reducing quantitative representation plasticheskih forms of cells, the appearance of foci of chondroid with piknomorfnymi chondrocytes, framing the damaged parts of the cartilage surface (Fig. Destruction of the subchondral bone and chondrogenic proliferate it. Hematoxylin and eosin. About. 10, OK. 8).

Using methods of polarization microscopy allowed us to identify compensatory transformations in the subchondral bone, which is expressed in the sclerosing bone beams located in proximity to areas with a higher degree of refraction of collagen (Fig. Carried by the subchondral osteosclerosis in the field of damage to the articular cartilage. Phenol reaction (reaction Ebner). About. 10, OK. 8, Fig. Carried by the subchondral osteosclerosis in the field of damage to the articular cartilage. Phenol reaction (reaction Ebner). About. 10, OK. 8), as well as increased content desulfation forms of glycosaminoglycans and hyaluronic acid, naturally increasing as it approaches the damaged departments (Fig. The content of glycosaminoglycans in the subchondral bone as it approaches the damaged sections. Toluidine blue. About. 10, OK. 8, Fig. The content of glycosaminoglycans in the subchondral bone as it approaches the damaged sections. Toluidine blue. About. 10, OK. 8). Identified especially the tee can testify actively flowing here the process of fibrillogenesis in comparison with dystrophic modified zones of bones, which have a high representation of chondroitin sulfates, the content of which, as is known, gives the most valuable information when determining the maturity and potential of cartilage cells. Significant changes destructive Genesis detected by metafigure cartilage, which is manifested in its hypertrophic development, the violation of the relation of structural zones, among which the most representative is the zone of monetary columns containing extensive cystic cavity and modified forms of the cells (Fig. Structural organization metafigure cartilage in terms of modeling extreme impact. Hematoxylin and eosin. About. 10, OK. 8). Pay attention to the hypertrophic zone and the destroyed cartilage (Fig. Structural organization metafigure cartilage in terms of modeling extreme impact. Hematoxylin and eosin. About. 10, OK. 8, Fig. Structural organization metafigure cartilage in terms of modeling extreme impact. Hematoxylin and eosin. About. 10, OK. 8). As shown by our study, the growth zone is distinguished by the heterogeneity of the distribution of different fractions of glycosaminoglycans area histological slice (Fig. The distribution of glycosaminoglycans in metafigure the cartilage in the simulation of extreme impact. Toluidine blue. About. 10, OK , Fig. The distribution of glycosaminoglycans in metafigure the cartilage in the simulation of extreme impact. Toluidine blue. About. 10, OK. 8), which may reflect the specific power loads falling on bone and its separate areas, as a result of damage to the latter in particular.

Thus, intra-articular injections of prednisolone at a dose of 15-25 ml 1-2 times in two weeks in the month lead to marked changes in the joints, paraartikulyarnye tissues, which can be classified as osteoarthritis of the III degree, flowing type of aseptic necrosis. However, the compensatory response of damaged tissues may indicate incomplete recovery and insolvency in biomechanical terms.

In a series of experiments with the introduction of the buffer in the joint after modeling the destructive process of pronounced differences from the above is not detected. In this regard, a comparative analysis conducted in the group of animals that have been simulated extreme effects of prednisolone followed by the introduction of glucosamine hydrochloride.

The structure of the joint components in terms of modeling the destructive process by intraarticular injection of prednisolone and actions of glucosamine hydrochloride.

The analysis of the histological data allowed us to conclude that the por is the application of glucosamine hydrochloride in dogs causes complex structural changes in the affected joint adaptation of Genesis, which ultimately leads to complete or partial recovery of functional fitness. So, a month after the first injection of the drug mobility was restored partially or completely. The affected limb performs state-locomotor function. Articular cartilage at a considerable distance retains Shine, while its dullness noted only near the area of the defect. Histological studies have shown that the area of the defect of the articular cartilage filled chondroitin proliferatum, the nature of which has a specific structural manifestations, due to the size of the damaged area (Fig. Androidmy proliferate in the articular cartilage after using glucosamine hydrochloride. Hematoxylin and eosin. About. 10, OK. 8, Fig. Androidmy proliferate in the articular cartilage after using glucosamine hydrochloride. Hematoxylin and eosin. About. 10, OK. 8). Cell populations regenerate represented mainly by hypertrophic chondrocytes, which may determine their oxytelinae tinctorially properties. Minor area of damage done oxyphyllum regenerate, do not differ in their morphological organization from the surface and intermediate zones of intact cartilage surface. In the territorial proximity of the regeneration zone is marked foci chondroid is, consisting of microforming chondrocytes (Fig. Substitution of the defect proliferatum. Foci of chondroid around the damaged area. Hematoxylin and eosin. About. 10, OK. 10).

If damage to the cartilage cover a significant area, the initial stage of its reparative regeneration is the formation of connective tissue regenerate, like linking undamaged areas of the articular surfaces (Fig. The initial stage of regeneration of articular cartilage after using glucosamine hydrochloride. Hematoxylin and eosin. About. 10, OK. 8), subregistration differentiated fibrous cartilage, the fibers of which are oriented in accordance with the lines of power loads experienced articular surfaces (Fig. Substitution of the defect fibrous cartilage after using glucosamine hydrochloride. Hematoxylin and eosin. About. 10, about 10, Fig. Substitution of the defect fibrous cartilage after using glucosamine hydrochloride. Hematoxylin and eosin. About. 10, OK. 10).

The result of reparative reactive transformations from cartilage is the gradual alignment of the contours of the articular surface and the formation of a holistic cartilage (Fig. The formation of cartilage after using glucosamine hydrochloride. Hematoxylin and eosin. About. 10, OK. 8, Fig. The formation of the e cartilage after using glucosamine hydrochloride. Hematoxylin and eosin. About. 10, OK. 8). However, it is important to emphasize that the newly formed articular cartilage has a tissue-specific features that distinguish it from those of intact animals. So, after using glucosamine hydrochloride cartilage covering the joint has the same structural zone, and the control individuals: superficial, intermediate and deep. When this surface area in the initial stages of the regeneration process has greater representation and structure, stereotypical characteristic of fibrous cartilage. But in the later stages of the regeneration process, it acquires the cell specificity of hyaline cartilage (Fig. The stages of formation of the surface zone of articular cartilage using glucosamine hydrochloride. Stage of fibrous cartilage. Hematoxylin and eosin. About. 10, about 10, Fig. The stages of formation of the surface zone of articular cartilage using glucosamine hydrochloride. Stage hyaline cartilage. Hematoxylin and eosin. About. 10, OK. 8). In addition, the intermediate zone consists mainly of single spaced chondrocytes, isogenic groups and capsules can only see in her deep divisions (Fig. Structural organization of articular cartilage after using glucosamine hydrochloride. Hematoxylin and eosin. About. 10, OK. 10).

In the analysis step is no orientational order of molecules in the intercellular substance using polarization microscopy shows that regenerate in the articular cartilage presents oriented collagen structure corresponding control individuals: tangentially at the surface and vertically in the intermediate and deep zones (Fig. The architectonics of the collagen structures in regeneriruyuschim cartilage after using glucosamine hydrochloride. Phenol reaction (reaction Ebner). About. 10, OK. 8, Fig. The architectonics of the collagen structures in regeneriruyuschim cartilage after using glucosamine hydrochloride. Phenol reaction (reaction Ebner). 10, OK. 10.). However, the refraction of this fibrillar protein heterogeneous area histological slice that may be associated with incomplete end processes interstitial differentiation of the newly formed cartilage. Comparative study of preparations stained with toluidine blue showed a high content in the articular cartilage vysokotarifitsirovannyh fractions of glycosaminoglycans, which allows to think about his biomechanical perfection (Fig. The distribution of glycosaminoglycans in the articular cartilage after using glucosamine hydrochloride. Toluidine blue. About. 10, OK. 8, Fig. Reparative regeneration of the subchondral bone after using glucosamine hydrochloride. Hematoxylin and eosin. About. 10, OK. 8). Studies allow the conclusion is th what a source of formation of cartilage regenerate after using glucosamine hydrochloride may be synovial pannus joint capsule, triggering in turn, the staging of reparative regeneration of articular cartilage, which includes stages fibrous and hyaline cartilage. It is also shown that glucosamine hydrochloride stimulates interstitial differentiation regenerate towards chondrogenesis, providing the specificity of the humoral surrounding articular surface.

In subchondral bone revealed reparative conversion, which consists in stasis and zapustevanie infested vessels, increasing the density of the lines of bonding, the increase in the quantitative representation of osteoblasts and osteocytes (Fig. The distribution of glycosaminoglycans in the articular cartilage after using glucosamine hydrochloride. Toluidine blue. About. 10, OK. 8, Fig. Reparative regeneration of the subchondral bone after using glucosamine hydrochloride. Hematoxylin and eosin. About. 10, OK. 8, Fig. Restore microarchitecture subchondral bone tissue after administration of glucosamine hydrochloride. Hematoxylin and eosin. About. 10, OK. 8). Deep divisions remain areas of destruction of the bone, however, near are active processes of regeneration in terms of the formation of a new the x generations bone (Fig. Reparative regeneration of the subchondral bone after using glucosamine hydrochloride. Hematoxylin and eosin. About. 10, OK. 8, Fig. Reparative regeneration of the subchondral bone after using glucosamine hydrochloride. Hematoxylin and eosin. About. 10, OK. 8). In addition, we identified an extensive zone chondroitinase proliferate in the subchondral bone, consisting of single chondrocytes, isogenic groups, exaggerated shapes and large areas of damaged cartilage (Fig. Structural organization chondroitinase proliferate in the subchondral bone. Hematoxylin and eosin. About. 10, OK. 8, Fig. Structural organization chondroitinase proliferate in the subchondral bone. Hematoxylin and eosin. About. 10, OK. 8). It can be assumed that similar patterns are gipertroficski developed metatithemi cartilage, performing the role of local reservoir chondrogenic formations. In the study of the growth plates shown increase their size in comparison with those of the animals taken for the norm. Only in some cases, it is still razvlechenie cartilage and destructive forms of cells (Fig. Structural organization metafigure cartilage after using glucosamine hydrochloride. Hematoxylin and eosin. About. 10, OK. 8, Fig. Structural organization metafigure cartilage after using glucosamine is hydrochloride. Hematoxylin and eosin. About. 10, OK. 8), however, over a large germ plates registered in the recovery of their structure and zonal differentiation (Fig. Structural organization metafigure cartilage after using glucosamine hydrochloride. Hematoxylin and eosin. About. 10, OK. 8). In addition, their composition is found to have a high content of keratan and chondroitin sulfates in comparison with destructive loci metafigure cartilage (Fig. Content vysokotarifitsirovannyh forms of glycosaminoglycans in metafigure cartilage after using glucosamine hydrochloride. Toluidine blue. About. 10, OK. 8, Fig. Content vysokotarifitsirovannyh forms of glycosaminoglycans in metafigure cartilage after using glucosamine hydrochloride. Toluidine blue. About. 10, OK. 8).

Thus, intra-articular injection of prednisolone at a dose of 15-25 ml 1-2 times in two weeks in the month causes knee pain in dogs sustainable picture of osteoarthritis of the III degree. It is characterized by complex structural transformations destructive Genesis in the joint and paraartikulyarnye fabrics, typical of necrosis. However, the intramuscular use of glucosamine hydrochloride in a dose of 15-25 mg 1-2 times per week during the month induces reparative processes in the articular cartilage, not inhibiting collagen synthesis, causing ulcero the military impact, providing stable chondromodulin effect that determines the phenomenon of reparation.

Quantitative study of the refraction of glycosaminoglycans (see table) in the data series and the assessment of the manifestation of compensation for the impact on the components of the joints this wayreduces the time of the survey and increases its accuracy, unifies impact assessment components of the joints in animals.

Sources of information

1. SU 1782547 A1, 5A 61 B 10/00, 23.12.1992.

2. Annefeld M. Veranderungen in der Ultrastructur der Chondrocyten unter dem Einflub a GAG-Peptid-Komplex / M.Annefeld, R.Raiss // Aktuelle Rheumatologie. - 1984. - S.2, Bd.9. - S.99-105.

Method of assessment manifestations of compensation for the impact on the components of the joints in animals in terms of modeling degenerative-dystrophic process, which consists in the fact that double-determine the refractive index of glycosaminoglycans, before and after the compensatory effects, and the formula to determine the diagnostic criterion is the ratio of the manifestations of compensation (PDA): PDA=CU2/CU1where KP1- coefficient of refraction of glycosaminoglycans impact KP2the refractive index after the compensatory effects, when the value of the PDA less than 1.5 give a negative assessment of compensatory manifestation, when the value of the PDA 1.5 and more manifestation to the mpensatio consider appropriate.



 

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2 ex

FIELD: medicine, gastroenterology.

SUBSTANCE: a patient should be tested, moreover, due to gas-chromatography technique one should detect salivary acetic, propionic and butyric acids and at concentration of any of these acids being: acetic acid above 0.910 mM/l, propionic acid above 0.150 mM/l and butyric acid above 0.013 mM/l one should diagnose chronic gastroduodenitis, and at concentrations of acetic acid ranged above 0.010 to 0.910 mM/l, propionic acid up to 0.150 mM/l and butyric acid up to 0.013 mM/l - functional dyspepsia. The innovation enables to increase objectivity and information value of the method suggested.

EFFECT: higher accuracy of diagnostics.

3 ex, 2 tbl

FIELD: biology, medicine, in particular histological investigation of shells with natural surface by using light-optical microscope.

SUBSTANCE: claimed method includes surface relief investigation by using plane field microscope in reflected light at one-side falling shadow-forming lighting. Light-reflecting ability of specimen surface is provided by silver impregnation. Said impregnation is carried out by specimen hold in 10 % silver nitrogen solution for 10 min followed by reducing thereof with 1 % ascorbic acid solution for 1 min.

EFFECT: method for investigation of natural shell surface relief of improved quality due to visual representation of surface three-dimension image on tissue level.

3 cl, 4 dwg

FIELD: molecular biology, gene engineering.

SUBSTANCE: invention relates to method for detection of typical marker foreign DNA sequences, usable in plant modification, in transgenic plant materials and products made of the same. Claimed identification method includes carrying out of multiplex polymerase chain reaction on the base of DNA isolated from tested material followed by hybridization of fluorescent labeled reaction products on specialized bioarrays. Hybridization data are registered by control and program complex and identification of foreign genes and regulatory zones thereof in tested material is carried out, and obtained results are quantitatively interpreted.

EFFECT: method for large scale screening of plant materials and products obtained from the same.

5 cl, 5 dwg, 2 tbl, 4 ex

FIELD: biotechnology.

SUBSTANCE: the present innovation deals with individual matching donor sheep to recipient sheep at similar antigenic composition of blood types being correspondent to the value of antigenic similarity index being ra=0.51-1.00, where ra - antigenic similarity index. Moreover, the mentioned antigenic similarity index should be calculated by the following formula: where S - the number of similar antigens in a donor sheep and in a recipient sheep, n1 - the number of detected antigens in a donor sheep, n2 - the number of detected antigens in a recipient sheep. The present innovation enables to increase the level of adaptability of transferred ovine embryos by 25%.

EFFECT: higher efficiency of embryo transfer.

3 ex, 4 tbl

FIELD: medical equipment.

SUBSTANCE: device can be used in microbiology. It characterizes by peculiarities of taking microorganisms from surface of nose polipi for direct electron microscope testing. Device has rod with cap. Rod is made of iron. Two-sided sticky carbonaceous band is fixed onto cap of rod. Top sticky surface of band is brought into contact with mucous of polipi of nose for getting preparation-print of micro content followed by viewing it in scanning electron microscope. Device doesn't cause traumatism of patient's mucous and allows determining L-shaped bacteria quickly as well as their sizes, shape and types of reproduction onto polyp tissue.

EFFECT: improved efficiency of detection.

3 dwg, 2 ex

FIELD: medicine.

SUBSTANCE: method involves carrying out clinical biological examination of a teenager. When finding sensory disorders manifested as distorted taste and smell perception related to iron deficiency in organism in patients periodically observed at the age of 10-15 years and/or at the examination time, risk of primary pathological attraction to psychoactive drugs is to be predicted.

EFFECT: high reliability of prognosis.

FIELD: medicine.

SUBSTANCE: method can be used for measuring concentration of glucose in sample's fluid as tissue fluid. For performing the method the measuring device is used which has working part of detector, second working part of detector and control part of detector. Fluid's sample is applied onto measuring unit and electric current being proportional to concentration of matter in sample's fluid is measured in any part of detector. Electric currents are compared for setting difference. If the difference exceeds preset threshold value, the index of error generates to show that volume of sample is not sufficient.

EFFECT: improved precision of measurement.

2 cl, 2 dwg

FIELD: veterinary medicine.

SUBSTANCE: one should twice detect collagen refraction coefficient: before and after compensatory impact and according to special formula it is possible to detect diagnostic criterion - coefficient of compensatory manifestation (CCM): where RC1 - collagen refraction coefficient before impact, RC2 - collagen refraction coefficient after compensatory impact, at CCM value being 0.83 and below one should provide positive evaluation for compensatory manifestation, and at CCM value being above 0.83 compensatory manifestation should be considered to be inadequate. The innovation enables to unify evaluation of the impact performed upon articular components in animals.

EFFECT: higher accuracy of evaluation.

49 dwg, 1 tbl

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