Strain bifidobacterium bifidum 79-92 used for preparing fermented-milk, fermented and nonfermented foodstuffs, ferments, hygienic and cosmetic agents, biologically active supplements and bacterial preparations

FIELD: microbiology, biotechnology, and food, cosmetic and medicinal industries.

SUBSTANCE: the strain Bifidobacterium bifidum 79-92 is isolated from the health nurse baby intestine content and deposited in the museum of microorganisms GU MNIIEM named for G. N. Gabrichevskiy at the code number № 184. The strain multiplies actively in nutrient medium and accumulates industrial biomass for short culturing period (12-24 h) with high concentration of bifidobacteria and shows acid-forming activity. Invention provides enhancing biological activity and resistance of the strain to conditions of environment in all temperature regions in cells development. Using the strain provides normalization of human body microbiocenosis, among them, in gastroenteric and urogenital tracts, cutaneous and mucosa tissues also, and expanding assortment of similar agents.

EFFECT: valuable properties of strain.

1 tbl, 4 ex

 

The invention relates to the food, cosmetic, biotech and medical industries, in particular, is used to prepare fermented milk, fermented and non-fermented food, hygiene and cosmetics, dietary supplements and bacterial preparations

Due to unfavorable environmental conditions, stress, prolonged antibiotic, radiotherapy and chemotherapy, dysbiosis resulting from illness, an increasing number of people suffering from disorders of the normal microflora. For the treatment and prevention of these disorders is gaining attention as drugs, with probiotic action, and food products containing bifidobacteria.

In the human intestine found 5 species of bifidobacteria - B.bifidum, B.longum, B.adolescentis, B.breve and B.infantis. With the greatest frequency in the biocenosis detected species B.bifidum, B.longum and B.adolescentis (39%-75%), and the last two are dominated by adults, and species B.breve and B.infantis - predominantly in infants (17%-36%).

Well-known foreign mono - and combination preparations-eubiotics and dairy products, including bifidobacteria species B.bifidum, B.lonqum, B.adolescentis, B.breve (patent 2336886, patent 2411008, France; patent 637297, Switzerland; patent 2134179, Pat the t 2421066, Germany; patent 05227946, patent 0430642, Japan; patent 4645667, USA).

In our country for the preparation of bacterial preparations and medical products-dietary use of domestic strains of bifidobacteria species B.bifidum No. 1; 791; PMBC S-1257, Ac-1578; B.lonqum VM; B.adolescentis MC-42, G 7513; .breve 79-119, B.infantis 79-43 (copyright certificate №343588, RF patent 1347221, patent 849779, RF patent 2108383, RF patent 2152993, patent 863639, RF patent 2120991, RF patent 2078814, RF patent 2111248).

The quality of medicinal products and drugs is largely due to the properties of the producer strain - specific activity and adaptability. A large range of biologically active and technologically advanced strains of bifidobacteria makes possible the development of new mono - and integrated products for medical nutrition and bakpreparatov, capable of providing high colonization resistance of the human intestine, to restore the normal microflora and reduce the incidence of various intestinal infections.

The present invention is to obtain a strain of microorganism of the species B.bifidum 79-92 with high biological activity, resistance to environmental conditions, with a stabilizing kislotoobrazovanie in the whole temperature range development of cells proliferating on artificial nutrient media.

Strain Bifidobacterium bifidum 79-92 kind of the len of the contents of the intestines of healthy infant and deposited in the Museum of microorganisms state mniam them. Hingamisega No. 184.

The inventive strain can be used as biologically active substances in the production of fermented food products, such as kvass, beer, etc., non-fermented foods, such as juices, drinks, etc., starter cultures for the preparation of dairy products and biologically active additives to food. Also, the strain of Bifidobacterium bifidum 79-92 can be widely used for the production of hygienic and cosmetic products, such as: creams, lotions, wipes, Soaps, powders, tampons, nourishing masks, gels, feminine sanitary pads, skin milk, various powders, etc.

In addition, the strain of Bifidobacterium B.bifidum 79-92 can be used in the production of bacterial preparations, in the form of candles, oral, and other tools.

The technical result of the claimed invention to provide a strain Bifidobacterium B.bifidum 79-92, which can be used as biologically active substances in the composition of fermented and non-fermented food starters, hygiene and cosmetics, dietary supplements and bacterial preparations. Strain Bifidobacterium B.bifidum 79-92 has biologically and technologically important properties, such as acid-forming activity and its ability to increase microbial biomass in short the e terms of cultivation. This will allow it to be used in the production of various probiotic means of ensuring the normalization of microbiocenosis of the human body, including the gastrointestinal and urogenital tracts, skin and mucous membranes of the skin, and broaden the Arsenal of similar means.

Cultural and morphological properties of the strain.

Cells are gr+ polymorphic sticks with bifurcation or thickening 1-2 ends, in a liquid nutrient medium to grow over the entire height of the volume, except for the zone of aerobiosis. Separately grown colonies on hepatic nutrient medium have cometabolism form colonies. Logarithmic growth phase ends for 12-24 hours.

Physical and biochemical properties of the strain.

Non-sporulating, still gone anaerobic. The optimum temperature for growing 37-39 degrees Celsius. Catalase does not form, gelatine does not dilute. Sprayway glucose with the formation of acetic and L + lactic acid, without the formation of gas, sakissa environment growing to a pH of 4.1-3.9 to. Ferments lactose, fructose, galactose, sucrose, maltose, variabelno: melibiose. Not ferments sorbitol, arabinose, salicin, Inositol, inulin, cellobiose, xylose, mannose, beckons. The strain increases microbial mass and increase hydrolysate-dairy medium (GM), Bifidum environment (BF), producing acids (table).

The accumulation of microbial biomass (CFU/ml lg10) and the activity of acid generating (in degrees Turner) strain 79-92 in the dynamics of development
NUTRITIONALTIME of CULTIVATION, BIOLOGICAL ACTIVITY
WEDNESDAY12 hours24 hours48 h
SOMESOMESOME
GM7,8768,61107,2140
BF8,0908,81008,0128

The maximum strain accumulates microbial mass to 12-24 hours, reaching in 1 ml lg 8-9 microbial cells that are actively producing acid.

Thus, the proposed strain .bifidum 79-92 tech as actively propagated in nutrient media with the accumulation of biomass production in short, 12-24 hours, timing of cultivation with a high concentration of bifidobacteria; has acid-forming activity. Fermented, unfermented food, hygiene, cosmetics, dietary supplements and bacterial p is aparati, containing strain .bifidum 79-92, can be widely used in food, medical, biotechnology and cosmetic industries.

The ability of strain .bifidum 79-92 to increase the biomass in the nutrient medium in a short period of cultivation, acid-forming activity, as well as other useful for the human body properties, making possible the use of the strain in the various corrective means of therapeutic and prophylactic purposes.

Example 1. The use of strain .bifidum 79-92 in the composition of dietary supplements.

1 passage: lyophilized strain .bifidum 79-92 in an amount of 0.1 g dry biomass contribute in tubes with nutrient medium and titrated by the method of ten-fold dilutions up to 10-9. Incubated at a temperature of 37-38 degrees Celsius before the formation of visible growth in the last dilutions.

2 passage: a grown culture of 10-7-9dilutions up to 10% contribute to the nutrient medium and cultured at a temperature of 37-38 degrees Celsius within 12-24 h before formation of the dense growth of the visible area of aerobiosis.

3 passage: the biomass in an amount up to 10% of the volume contribute to the accumulation and cultivate 12-24 h at 37-38 degrees Celsius to obtain a biomass content of live bifidobacteria not less than 108/ml.

The result is biologically aktivno the additive, which is a liquid concentrate with a high, not less than 108/ml, content of bifidobacteria.

Example 2. The use of strain Century 79-92 for the preparation of fermented and non-fermented foods.

1 passage: lyophilized strain .bifidum 79-92 in the amount of 0.1 g dry biomass contribute in tubes with nutrient medium and titrated by the method of ten-fold dilutions up to 10-9. Incubated at 37-38 degrees Celsius before the formation of visible growth in the last dilutions.

2 passage: a grown culture of 10-7-9dilutions up to 10% contribute to the nutrient medium and cultured at a temperature of 37-38 degrees Celsius within 12-24 h before formation of the dense growth with a highly visible area of aerobiosis.

3 passage: the biomass in an amount up to 10% of the volume contribute to the accumulation and cultivate 12-24 h at 37-38 degrees Celsius to obtain a biomass content of live bifidobacteria not less than 108/ml.

Obtaining a fermented or non-fermented food: grown biomass bring in food raw materials (milk, juices, beer, kvass and other), stirred and poured into the vessel. The result is a product with a concentration of live bifidobacteria not less than 106/ml.

Example 3. The use of strain .bifidum 79-92 to obtain bacterial preparations.

Cultures of the strain of the 1st or 2nd passage (example 1) contribute to the nutrient medium, cultivated at a temperature of 37-38 degrees Celsius to obtain the biomass of bifidobacteria with the contents of living cells is not less than 108in 1 ml. To the obtained biomass add a protective environment drying (saharso-gelatin medium, skim milk). The resulting suspension is poured into ampoules, vials and other containers, frozen at a temperature of minus 35 to 45 degrees Celsius and lyophilizers from the frozen state in a vacuum drying apparatus. The resulting preparation contains not less than 107living cells of bifidobacteria. The content of bifidobacteria is consistent with the scientific and technical documentation on similar bifidobakterii drug - Bifidumbacterin dry (FS 42 132 g - 94) and is sufficient to use the funds received as a drug-eubiotics.

Example 4. The use of strain Century 79-92 to get hygienic and cosmetic products.

The culture of the strain of the 1st or 2nd passage (example 1) contribute to the nutrient medium, cultured at a temperature of 37-38 degrees Celsius to obtain the biomass of bifidobacteria with the contents of living cells is not less than 108in 1 ml.

The resulting biomass of bifidobacteria strain .bifidum 79-92 introduced into the hygienic or cosmetic products.

Strain Bifidobacterium bifidum 79-92 (collection No. 184 state collection of ICRI the flora of man GCNM state mniam health Ministry to them. Hingamisega)used to obtain fermented milk, fermented and non-fermented food starters, hygiene and cosmetics, dietary supplements and bacterial preparations.



 

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EFFECT: valuable properties of strain.

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1 tbl

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1 tbl

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EFFECT: valuable biological and medicinal properties of polypeptide.

3 dwg, 4 ex

FIELD: microbiology, biotechnology, and food, cosmetic and medicinal industries.

SUBSTANCE: the strain Bifidobacterium bifidum 79-36 is isolated from the health nurse baby content, and deposited in the museum of microorganisms GU MNIIEM named for G. N. Gabrichevskiy. The strain multiplies actively in nutrient medium and accumulated industrial biomass for short culturing period (18-24 h) with high concentration of bifidobacteria and shows acid-forming activity. Using the invention allows preparing fermented and non-fermented foodstuffs, biologically active supplements, bacterial preparations, hygienic and cosmetic agents. Their using provides normalization of human body microbiocenosis, among the, gastroenteric and urogenital tracts, cutaneous and mucosa tissues.

EFFECT: valuable properties of strain.

1 tbl, 4 ex

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