Encapsulation of polypeptides in starch matrix
FIELD: biotechnology, peptides, genetic engineering.
SUBSTANCE: invention relates to constructing nucleic acid molecule comprising functional in starch-containing plant tissues promoter, fragment encoding transit peptide for translocation of useful peptide into amyloplast, fragment encoding useful peptide, region encapsulating into starch and terminator. In insertion into plant genome DNA molecule expresses hybrid polypeptide comprising the desirable protein encapsulated into starch matrix. Prepared vegetable material can be used, for example, in manufacturing fodders for mammals, fishes and poultries. Also, invention can be used in food industry.
EFFECT: valuable properties of nucleic acid and polypeptides.
15 cl, 19 dwg, 9 tbl, 7 ex
FIELD: biotechnology, in particular method for construction and production of mutant transglutaminases (MTG).
SUBSTANCE: invention relates to method for construction and production of mutant transglutaminases based on space structure of transglutaminase obtained from Streptoverticillium mobaraense, as well as to mutant MTG obtained by said method. Also disclosed are method for MTG modification based on space structure and modified transglutaminase with enhanced reactivity relative to substrate. Method of present invention makes it possible to predict MTG binding site to substrate on the base of space structure that is determined by MNG crystal X-ray analysis, and to design mutant transglutaminases by replacement, insertion or deletion of amino acid residues disposed on transglutaminase substrate-binding site.
EFFECT: new method for production and modification of mutant transglutaminases.
6 cl, 60 dwg, 5 ex
FIELD: biotechnology, biochemistry, microbiology.
SUBSTANCE: the strain Bacillus circulans B-65 a producer of cyclodextrin glucanotransferase is isolated and selected form the soil sample by culturing in nutrient medium with amylolytic activity 12.17 U/ml and cyclodextrinogenic activity up to 0.530 U/ml. Cyclodextrin glucanotransferase isolated from B. circulans B-65 shows the high degree for conversion of starch to cyclodextrins and this enzyme is specific for formation of β-cyclodextrin. Invention can be used in food industry for preparing cyclodextrins and cyclodextrin glucanotransferase used in different branches of industry.
EFFECT: improved isolating method, valuable properties of strain and enzymes.
9 cl, 8 tbl, 2 ex
FIELD: gene engineering, in particular plant selection.
SUBSTANCE: chimera gene encoding protoporphyrinogen oxydase is introduced into plant.
EFFECT: plants transformed by chimera gene and generation thereof with herbicide resistance.
16 cl, 2 tbl, 27 ex
FIELD: biotechnology, agriculture.
SUBSTANCE: invention relates to a system used for eradication of vegetable cells. Invention involves construction of the biological functional expression vector that carries a chimeric gene comprising promoter associated functionally with a nucleotide sequence that encodes the combination of α- and β-domains of maize inactivating mature protein RIP. Then method involves carrying out the transformation of the plant. Invention provides effective removal of required cells in the plant organism.
EFFECT: improved and valuable properties of system.
21 cl, 15 dwg, 3 tbl, 21 ex
FIELD: genetic engineering, agriculture.
SUBSTANCE: invention relates to a method for preparing transgenic plants. Method involves selection of the strain of the required plant, preparing mother plants and carrying out the multiplication of the vegetable material for preparing leaf explants. Then method involves making a vector for transfer of genetic material expressing the end protein that enhances resistance of plants against phytopathogens followed by the agrobacterial transformation of explants with agrobacterium Agrobacterium tumifaciens and the following selection of transgenic tissue and multiplication of transformants. At the transformation stage method involves stage-by-stage co-cultivation of explants with steps amount taken in the range from 2 to 5 and wherein leaf disks with the ratio of cut length to the area disk is in the range 0.1-0.2 mm/mm2 are used as explants. Invention provides preparing transgenic plants showing the enhanced resistance against phytopathogens and also to enhance frequency of the transient expression, frequency in formation of transgenic tissues, frequency in regeneration of transgenic sprouts, part of direct transformants and to reduce frequency of somaclonal alterations.
EFFECT: improved preparing method, valuable properties of transgenic plants.
5 cl, 9 dwg, 12 tbl, 16 ex
FIELD: biotechnology, in particular production of genetically modified plants with altered level of one metabolite secondary product.
SUBSTANCE: claimed method includes selection of nuclear acid sequence encoding enzyme, which is absent in secondary metabolite process, but is capable to modify utilization of intermediate substrate in secondary metabolite process connected with plant nutrient profile. Then recombinant molecule is constructed, which contains abovementioned sequence, to transform plant cell. From this cell genetically modified plant is regenerated. Obtained plant is useful in animal feed preparation.
EFFECT: plants with high nutrient value and improved quality.
35 cl, 33 dwg, 22 ex
FIELD: biotechnology, in particular isolated DNA molecule providing plant disease resistance and method for providing of disease resistance to plants.
SUBSTANCE: DNA molecular containing N1M1 is isolated. Recombinant vector including active in plant promoter functionally bonded with said DNA is constructed and plant is transformed by this vector.
EFFECT: decreased technological charges and increased land productivity.
9 cl, 37 dwg, 18 tbl, 10 ex
SUBSTANCE: protein substances are produced from cultivation medium unlike prior art where they are produced by reprocessing of plant tissue. Application of moss protonema as plant tissue makes it possible to isolate therefrom heterologous proteins in active form without degradation of productive tissues and cells.
EFFECT: improved method for production of protein substances.
FIELD: genetic engineering, biotechnology, biochemistry, agriculture, food industry, medicine.
SUBSTANCE: invention relates to the transformation of plant with nucleic acid encoding enzyme Δ6-desaturase in C. elegans that results to preparing a plant with enhanced content of gamma-linolenic acid and resistance to cold. Desaturase extracted from the plant can be used for preparing a drug used for treatment of disorder in body associated with deficiency of gamma-linolenic acid in it.
EFFECT: valuable biological properties of genes and desaturases.
36 cl, 9 dwg, 2 ex
FIELD: biotechnology, molecular biology, biochemistry.
SUBSTANCE: invention relates to regulatory sequences. Method involves isolation of DNA molecule with nucleotide sequence SEQIDNO:2 or SEQIDNO:3 that is necessary for expression of the required encoding sequence. Then vector comprising any of indicated sequences and the required sequence is constructed followed by transformation a plant with the prepared vector. Invention provides preparing transgenic plants with regulating expression of the required gene.
EFFECT: improved preparing method.
19 cl, 1 tbl, 6 ex
FIELD: genetic engineering, pharmaceutical and medical-biological industry.
SUBSTANCE: invention proposes a chimeric sequence of nucleic acid encoding a fused polypeptide able to bind with the vessel endothelium growth factor (VEGF) and to inhibit its specific mitogenic effect. The fused polypeptide molecule comprises immunoglobulin-like domain 2 of VEGF-receptor Flt1, immunoglobulin-like domain 3 of VEGF-receptor Flk 1 or Flt4 and multimerizing component represented by either domain Fc IgG or heave chain IgG. By expression of the proposed chimeric sequence or its two successively joined copies in a host-cell a monomer or dimer of the fused polypeptide are prepared, respectively, that can be used for suppression of VEGF activity in mammals, in particular, in humans. New VEGF inhibitors differ from the known one by the improved pharmacokinetics.
EFFECT: improved preparing method, valuable biological properties of polypeptide.
23 cl, 67 dwg, 1 tbl, 35 ex