Preparation for decreasing protein deficiency in body and method for preparing such preparation

FIELD: medicine, biomolecular pharmacology.

SUBSTANCE: invention relates to the preparation that represents the product of biological and chemical treatment of vegetable biomass or its waste by using microorganisms. The parent raw represents carbon-containing biomass of vegetable origin wherein carbohydrate moiety represents 5% of the total biomass, not less. Biomass is mixed with water with addition of lactic acid microorganisms taken as a standard ferment with the parent content 0.2-0.5% per 1 l of an aqueous mixture. The mixture is incubated at 40°C for 6 h, not less, under condition of maintaining pH value at the level 6.75 ± 0.05 by addition of ammonium hydroxide. The prepared paste-like mass can be used as the assimilable protein precursor. Also, invention involves characterization of a method for preparing the preparation that involves treatment of the parent vegetable biomass with lactic acid microorganisms and fractional addition of ammonium hydroxide to provide pH value 6.75 ± 0.05. The final product represents the mass comprising the protein assimilable precursors. Invention provides the additional source of assimilable protein precursors.

EFFECT: improved preparing method, valuable properties of preparation.

6 cl, 7 ex

 

The invention relates to the field of biomolecular pharmacology, namely the creation of a drug that can reduce protein deficiency in the body of a mammal, as well as to a method for producing such a product.

For normal growth and development of a mammal, including a human body needs proteins of plant and animals. First he gets with plant food, the second with the animal. According to the world Health organization (who), the food more than half the population of our planet cannot be considered satisfactory because of the lack of protein. Global shortages of food protein is equal to 1.5 million tons per year. This problem is caused, including, and impaired ecosystem habitat of mikroorganizmov [1].

To resolve the shortage of food and fodder protein (protein fasting) it is necessary to increase the productivity of crop and livestock production through environmentally friendly products. Plants, absorbing energy from sunlight (photosynthesis), turn it into the energy of chemical bonds, providing a relatively simple environmental substances (carbon dioxide, water, mineral salts) synthesized "de Novo" carbohydrates and amino acids, and from them build their cellular proteins.

Animals can synthesize amino acids and carbohydrates are "de Novo" is not given. Both they get eaten by them from the plant is th. The main source of amino acids for the synthesis of animal protein are amino acids and some other nitrogen-containing substances (amino sugar, aminoalcohols, nitrogenous bases, some ammonium salts of organic acids and other substances).

Nitrogen-containing substances of animal feed that can be included in the composition of animal protein, referred to as "crude protein".

To determine the quantitative content of crude protein in the stern on the percentage of elementary nitrogen multiplied by 6,25.

If you have a low content of crude protein in the feed ration of the animal - "protein starvation", produced little animal protein, animal slowly grows and develops, weakly resists the adverse effects of the environment, often ill and dying compared to those animals that receive crude protein in a physiologically obuslovennom normal.

Usually to improve livestock productivity and ensure a high level of average daily liveweight gain in the feed is injected rich in protein components (peas, beans, soy, yeast, meat and/or fish meal and other) These components of the road and often scarce, sometimes dangerous (meat and bone meal may be infected with a virus, "mad cow disease").

In some cases, to feed cattle BBO is Yat urea (carbamide), because it can be transformed by microorganisms of the digestive tract in amino acids and thus to increase the overall pool of amino acids in the animal body (see, for example, patent RF №2097985, 1997). But add it to the daily feed ration is possible only in small quantities - about 150 g per head. Overdose leads to poisoning and sometimes death of the animal.

Feed scarcity in agriculture is forcing scientists and practitioners to find new, unconventional sources of recharge. One of them is wood and waste generated during harvesting and processing. The products of partial hydrolysis of wood can be successfully used to replace part of the volume of traditional feedstuffs in diets of ruminants (see RF patent №2088106, 1997).

In France, Canada and other countries have created a major installation of continuous action, which by processing of plant biomass vapor pressure (30 ATM) is food "ProCell". Preparation and use of plant protein feed used in Russia for quite a long time [2].

In many countries of the world are grown an enormous amount of plant mass, in which the carbohydrate content is much higher than the protein content. For example, the ratio in grapes 43:1; apples 30:1; berries 10-15:1, potatoes, turnips, Swedes and other corn is the fruit of about 10:1, sugar cane 25:1. Such vegetation can not effectively be used as animal feed, as well as protein and nutritious foods because of the low content of digestible protein. Processing biomass of these and other plants using special technology could produce products with the desired ratio of carbohydrates and protein for feed and food purposes.

The conversion of carbohydrates from plant matter into digestible by the body of a mammal precursor protein can turn them into full-fledged, meet the requirements of intensive livestock feed, and if additional cleaning and food product.

In the Russian Federation developed the scheme of complex processing of wood to increase output of products, digested by microorganisms. Wood is subjected to alkaline hydrolysis in an aqueous solution of NaOH at 175°C for 2 h while the dissolution occurs approximately 59% limnogeology complex, the cellulose residue and liquor containing organic acids and carbonyl compounds. Cellulose hydrolyzing the residue with sulfuric acid. This is followed by neutralization of the alkaline liquor acid hydrolysate, Department of lignin, the crystallization of sodium sulfate by adding alcohol afuraldehyde fracc and and separating it from the solution. The solvent is distilled off, and the substrate is diluted with water to a 1%concentration of monosaccharides and organic acids and add nutrient salts [3].

The closest analogue to this development can be considered patent RF №2084175, 1997 disclosed In the patent technology of production of animal feed from waste plant. However, this development cannot be used as a technology for preparing a drug that can reduce the deficit of protein that is essential for mammals.

The technical task of the present invention is a drug that can reduce and/or eliminate the deficiency of protein in the body of a mammal, and a method of obtaining such a drug.

The technical problem is solved by the fact that developed the drug to reduce protein deficiency in the body, which is a product of biological and chemical processing of plant biomass or waste with the participation of microorganisms, while the feedstock is carbohydrate-containing biomass of plant origin, in which the carbohydrate portion is not less than 5% of the total weight of the feedstock, biomass is mixed with water and treated with lactic bacteria, taken in the form of a standard yeast (e.g., MT) when the initial content of 0.2 to 5% for each liter of water mixture. Incubus is by the mixture under physiological conditions is not less than 6 hours while maintaining the pH level of 6.75± 0,05 by adding ammonium hydroxide. The resulting pasty mass can be utilized by the body of a mammal as digestible precursor protein, called protein.

As a source of raw materials can be used biomass is selected from the group: silo dust grain crops, sugar beet pulp, grape pulp, crushed seed shells, waste milk production, chopped straw, shredded branches of non-toxic plants, watermelon and melon peel, stalks, and cobs of corn, cotton bolls.

Another object of the invention is a method of producing the drug, which is as follows.

The method of producing drug for the reduction of protein deficiency in the body by biological and chemical processing of plant biomass or waste with the participation of microorganisms in the quality of raw materials used carbohydrate-containing product in which the carbohydrate part of it is not less than 5%, raw materials are crushed and mixed with water, the treatment is carried out lactic acid bacteria, taken as a standard starter culture, such as MT, with the initial content of the leaven of 0.2 to 5.0% on each litre of water mix, incubate the mixture at physiological conditions for at least 6 hours, maintaining the pH level of 6.75±0,05 making the ammonium hydroxide, PEFC is the stabilization of the specified pH making hydroxide ammonium stop, and water mixture is evaporated to obtain a pasty mass, which represents the precursor protein, digestible, if necessary, the mass is dried for storage.

When processing coarse initial biomass more ground and to make the ICD is subjected to acid hydrolysis when heated. Acid hydrolysis is carried out at a pH of 1.0, a temperature of 90±0,5°C for a 2.0±0,1 hours using concentrated sulphuric acid.

When processing coarse initial biomass can be applied and alkaline hydrolysis, for example, wood is subjected to alkaline hydrolysis in an aqueous solution of NaOH at 175°C for 2 h, while the dissolution occurs about 60% limnogeology complex.

The invention is illustrated by the following examples.

Example 1 (experiment with dietary sugar)

In a glass cuvette with a volume of 3000 ml contributed 2.0 liters water, 100 grams of refined sugar (food), after dilution were incubated at a temperature of 40°together with a suspension of dry sourdough lactic acid bacteria MT (producer - Moscow experimental dairy plant). The cuvette was placed in a water bath with T°C=40°. the pH content of the cell - 6,95. Took a sample to determine the % SP and % amino acids. After 2 hours the pH of the cell to 6.0. The total amount of amino acids=0,66%.

Next, in small portions was added 10%solution of ammonia, aderiva pH of 6.75. The total amount of added ammonia solution and 170 ml. of the resulting preparation in the form of a suspension in a quantity of 50 ml were transferred to the laboratory feeds and feed additives research Institute feed them. Virvalasi to determine the % content of SP.

The results of the analysis: the concentration of SP in solution at normal humidity and 4.4%.

Conclusion: In this experiment, 100 g of sugar obtained 88 g of crude protein.

Note: Theoretically, 100 g of sugar you would get: 97,2 g SP., i.e. the practical release of SP from theoretical amounted to 90.5 per cent.

When thickening the resulting suspension to 40% of the original is obtained pasty mass containing 29.8% of the JV, which corresponds to 74.5% of the JV on the dry substance.

Example 2 (experiment with cheese whey)

In a glass with a capacity of 2 litres, containing 1 kg of cheese whey (pH 4,6; acidity 80°T, lactose - 4.1 percent), introduced 100 g of sugar, the experiment was performed as in example 1.

A glass of whey, sugar and yeast was placed in a water bath. Measured pH. pH 4.5. With stirring was added 5.6 ml of 10% solution of ammonia to bring the pH of the resulting suspension to 6.75.

Then during the whole process was added 10% solution of ammonia in small portions to maintain the pH level of 6.75±0,05.

By the end of the digestion process to the original volume of the sugar solution in serum was added 265 ml of 10% rest the RA ammonia.

Experimental fluid in a quantity of 50 ml was transferred to the analysis in the research Institute of feed them. Williams. The analysis showed that the content of SP in the sample is equal to 13.2%. When the concentration of the resulting suspension via vacuum evaporator to 40% in the obtained mass was contained 29.7% of the JV, calculated on the dry matter - sampled at 74.25% of the JV.

Example 3 (experiment with silo waste)

In a beaker with 800 ml contributed 25 g of the Elevator wastes 4th category (respiratory dust). Added 300 ml of water and 2 ml of concentrated sulfuric acid, bringing the pH to 1.0. The beaker was placed in a water bath at a temperature of 90°C. After 2 hours exposure was cooled to 40°C. Sulfuric acid was neutralized with a 10% solution of ammonia in water, bringing the pH to 6.75. Made sourdough ICD. Measured pH. pH of 4.8. Brought the pH up to 6.75, adding 0.2 ml of a solution of ammonia in water. As a souring content of glass making small portions of 10%ammonia solution, maintaining the pH level of 6.75±0,05. Total volume of 10%ammonia solution consumed for the neutralization formed during lactic acid fermentation was 9 ml. Resulting in the end of the process mushy mass was dried at a temperature of 50°With, brought to constant weight equal to 25 g, to grind to powder. Then 10 g of the obtained product and 10 g of the original silo waste sent to a laboratory soot nicesly evaluation and feed livestock research Institute feed them. Williams.

The analysis results show that the content of SP increased from 5.4% in the original product to 21.4% in the treated product, and the total content of amino acids with 26,92 g/kg to 44,72 g/kg, respectively.

Example 4 (experiment with the sugar beet pulp)

Dry sugar beet pulp in the amount of 25 g was placed in a glass beaker with a capacity of 500 ml, was added 300 ml of water and 2 ml of concentrated sulfuric acid, bringing the pH content of up to 1.0. The beaker was placed in a water bath with a temperature of 90°C, after 2 hours exposure was cooled to 40°and placed in a water bath at a temperature of 40°C. was Added a 10% solution of ammonia to bring the pH up to 6.75. Made sourdough ICD (Dairy pilot plant, Moscow). Measured pH. the pH of 4.9. Added 50 ml of milk. Brought the pH content of the glass to 6.75, adding 0.3 ml of 10% solution of ammonia in water. As a souring the contents of the glass made in small portions a solution of ammonia. Total ammonia solution consumed for the neutralization produced during the fermentation of lactic acid was 63 ml

Resulting in the end of the process pasty mass was dried at a temperature of 50°With, brought to constant weight 25 g, to grind to powder. Then 10 g of the obtained product and 10 g of original beet pulp was sent to the laboratory animal evaluation and feed agricultural alive is the shaft at the research Institute of feed them. Williams. The results of the analysis showed that the content of SP increased from 8.4% in the source to 47.1% in the resulting product.

Example 5 (experiment with potatoes)

The potato tuber to grind using a laboratory homogenizer. Then, 160 g of the resulting mass was transferred into a glass with a capacity of 0.8 liters. Added 350 ml of water, was added 2 ml of concentrated sulfuric acid to bring the pH of the solution to 1.0. Placed in a water bath at 90°C. After 2 hours exposure of the contents of the glass was cooled to 40°C. Made a ferment, as in example 4, was added 0.5 ml of 10% solution of ammonia in water to bring the pH up to 6.75±0,05. Then as a souring the contents of the glass and lowering the pH to 6.5) was added 10% solution of ammonia in an amount necessary to bring the pH up to 6.75±0,05. For the whole process to the point where the formation of lactic acid was stopped, the pH titrated mixture has ceased to decline, was spent 40 ml of 10% solution of ammonia in water. The resulting mass is dried, brought to constant weight 28 g and grind to a powder. Then 10 g of the obtained powder was transferred to the analyses in the research Institute of feed them. Williams for determining the content of crude protein and amino acids in the obtained sample.

The results of the analysis: 45.5% of the JV, including 6,47% of the total amino acid content.

Example 6 (experiment with straw)

In a beaker with 800 ml of the bore 25 g chopped straw of winter wheat. Added 300 ml of water and 2 ml of concentrated sulfuric acid, bringing the pH to 1.0. The beaker was placed in a water bath at a temperature of 90°C. After 2 hours exposure was cooled to 40°C. Sulfuric acid was neutralized with a 10% solution of ammonia in water, bringing the pH to 6.75. Made sourdough ICD. Measured pH. pH of 4.8. Brought the pH up to 6.75, adding 0.2 ml of a solution of ammonia in water 10%concentration. As a souring content of glass making small portions of 10% ammonia solution, maintaining the pH level of 6.75±0,05.

Resulting in the end of the process mushy mass was dried at a temperature of 50°With, brought to constant weight equal to 25 g, to grind to powder. Then 10 g of the obtained product and 10 g of the original straw of winter wheat was sent to the laboratory of the Institute of animal feed them. Williams. The analysis results show that the content of SP increased from 3.2% in the original product to 14.3% in the finished product.

Example 7 (serum+molasses)

In a glass with a capacity of 2 litres, containing 1 kg of cheese whey (pH of 4.6, acidity 80°T, lactose content of 4.1%, protein - 0,6%SV - 5,2%) made 154 g of molasses (DM content of 80%, sucrose - 43%, a pH of 6.7) was heated to 90°C, after 15 minutes, cooled to 40°after which he made a 20 ml fermentation ICD (Moscow experimental plant). A glass of whey, molasses and yeast was placed in a water BA is th at a temperature of 38° C. Measured pH. pH 4.5. With stirring was added 6 ml of an aqueous solution of ammonia to bring the pH of the mixture to 6.75. Then during the whole process was added 10% solution of ammonia in small portions to maintain the pH of the suspension at the level of 6.75±0,05. By the end of the fermentation was added 183 ml of ammonia solution. The result is a 94.2 g JV.

Thus, a drug that can reduce and/or eliminate "protein hunger and technologies for preparation is an important step for the development of a huge plant biomass that exists on the planet, which can be successfully used as an additional source of digestible protein precursors to solve the problems of the world shortage of protein.

References

1. Vmichael and other "environmental Health: practice evaluation", Moscow, 2000

2. "The production and application of food processing waste wood", Moscow, "Forest industry", 1978

3. Minankov, Dgupanovsky "Theoretical bases of technology of microbiological production", Moscow, Agropromizdat", 1990

1. Medication to reduce protein deficiency in the body, which is a product of biological and chemical treatment of biomass or waste with the participation of microorganisms, characterized in that the feedstock is carbohydrate-containing biome is soy vegetable or animal origin, while the carbohydrate part is not less than 5% of the total initial biomass, the biomass is mixed with water with the addition of a starter culture of lactic acid bacteria during its initial content of 0.2 to 5.0% for each liter of the aqueous mixture, incubated the mixture at 40°With not less than 6 hours while maintaining the pH level of 6.75±0,05 by introducing ammonium hydroxide, the resulting pasty mass is a digestible precursor protein.

2. The preparation according to claim 1, characterized in that the feedstock used, the biomass is selected from the group: silo dust grain crops, sugar beet pulp, grape pulp, crushed seed shells, chopped straw, shredded branches of non-toxic plants, watermelon and melon peel, stalks, and cobs of corn, cotton bolls, waste milk production.

3. The method of producing drug for the reduction of protein deficiency in the body by biological and chemical treatment of biomass or waste with the participation of microorganisms, characterized in that the feedstock use of carbohydrate-containing biomass of plant or animal origin, while the carbohydrate part of it is not less than 5%, raw materials, if necessary, crushed and mixed with water, the treatment is carried out lactic acid bacteria (ICD), taken in the form of the leaven of the PR is the initial content of the leaven of 0.01 to 5.0% for each liter of the aqueous mixture, incubate the mixture at 40°With not less than 6 hours, maintaining the pH level of 6.75±0,05 making ammonium hydroxide, after stabilization of the specified pH of the aqueous mixture is evaporated to obtain a pasty mass, which represents the absorbed precursor protein, optionally dried for storage.

4. The method according to claim 3, characterized in that the processing of the coarse initial biomass additionally crushed and before submitting ICB is subjected to acid hydrolysis when heated.

5. The method according to claim 4, characterized in that the acid hydrolysis is carried out at a pH of 1.0, a temperature of 90±0,5°C for a 2.0±0,1 h using concentrated sulphuric acid.

6. The method according to claim 3, characterized in that the processing of the initial biomass is conducted additionally alkaline hydrolysis conditions at temperatures above 100°C.



 

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