Method for producing of protein feed additive from raw plant material and apparatus for performing the same

FIELD: feed mill industry.

SUBSTANCE: method involves treating sunflower oilcake with catholyte; removing treated solution; extracting protein; filtering; drying sediment; grinding; simultaneously with treatment of sunflower oilcake with catholyte, providing treatment of soya with anolyte, with catholyte and anolyte circulating at equal velocities. Apparatus has two chambers connected with each other through semi-permeable partition. Each of said chambers is equipped with electrodes and dc source. Apparatus is further equipped with collecting chamber and pumps with pipelines.

EFFECT: increased efficiency of method and apparatus, reduced production time and decreased costs of additives.

1 dwg, 1 tbl

 

The invention relates to agriculture, in particular to the preparation of feed from plant products.

A known method for separating protein from sunflower meal by extraction of the protein in an alkaline solution, passing the extract through ultrafiltrate membrane, and precipitation of the protein with acid, all operations were carried out in an inert atmosphere (Sources of food protein/ Under. edit Vincitori. M.: Kolos, 1979. S).

However, this method cannot be used for industrial production of proteins from the meal, as its implementation requires expensive equipment (block ultrafiltration, a device for passing an inert gas), all this considerably increases the cost of the technology and the end product. In addition, it is doubtful that under these conditions it is possible to get protein from a meal with a high yield. This precludes the possibility of using this technology to obtain a protein concentrate from sunflower meal used in feed production.

A method of obtaining protein from sunflower meal, including extraction of protein from sunflower meal solution containing sodium chloride, then with a solution containing sodium hydroxide, separating the insoluble precipitate, precipitation with hydrochloric acid, drying spray enoy the dryer the air, and insoluble sludge cake is dried and used for animal feed. (Technology of food industry / edited Kowalska L.P. M.: Kolos, 1997. S, 463).

However, in the known method of protein meal is extracted in stages: first, an aqueous solution of sodium chloride and then with sodium hydroxide, resulting in large losses when using several extractants in connection with multi-stage process and requires a large number of technological solutions. In addition, the use of aggressive reagents complicates the use of these methods in production. One option to exclude corrosive chemicals technology is the using of electroactivated water.

The known method of using of electroactivated water for processing biological objects, such as hatching eggs (Method. recom. on the application of electroactivated water in the production of broiler meat / edited Vigilance, Vgos. - Zagorsk, 1990. P.34. - prototype), including the processing of biological object electroactivated mineralized water in the cathode zone of the diaphragm electrolyzer by immersing it in the solution, pH of which is within 9,5-11,0, followed by immersion in the anolyte with a pH value from 2.0 to 1.2 and aging in the liquid.

However, in the world is " a way Catolica cannot be processed biological object without removing it from the cathode zone of the diaphragm electrolyzer, because this violates the biological quality of the object. At the same time, in our case the biological object can be processed directly in the diaphragm cell, which reduces the number of technological operations. In addition, for our biological object, there is no need to use such high values of pH, as in the prototype. This reduces processing time and reduce the necessary electricity. With the reduction of the processing time is reduced, the temperature of the working solution, the increase of which adversely affect the extraction of the protein in solution, as it causes its coagulation.

A method of obtaining protein concentrate, comprising processing a biological object electroactivated mineralized water in the cathode zone of the diaphragm electrolyzer, as a biological object using belaustegui raw materials, such as meal or sunflower meal, and processed within 2-4 min to pH 7-8, then remove the treated solution from the electrolytic cell, extracted protein within 40-60 min, filtered and bring the extract solution of mineral acid to the isoelectric point of proteins, and the resulting precipitate is dried and crushed (Patent RF №2195836, class. And 23 K 1/00, 10.01.2003, bull. # 1 - prototype).

However, in the known method is used Odie is a source of raw materials, which does not allow to obtain high-quality and balanced amino acid composition additive. In addition, the high cost of energy per unit of mass production make it expensive. As a result of receiving supplements of the proposed method produces large amounts of waste (sewage), not used in production that pollutes the environment and also increases the cost of the additive.

A device for processing straw for animal feed. The device comprises a receptacle containing an alkaline environment, the capacity with acidic and located between a semi-permeable partition. The electrodes are respectively vessels with acidic and alkaline medium and is connected to the constant current source. Elastic cameras installed using rails to the opposite electrodes of the tank walls. The front walls of the chamber are movable with the possibility of reciprocating motion in the direction of the semi-permeable walls. Straw is loaded into the vessel with the alkaline medium, remove, allow to drain and immerse in a container of sour environment. At the same time in the tank with the alkaline medium placed a new batch of straw. While watching sustaining environments in containers by increasing or decreasing the elastic cameras with movable rails fixed to the vertical supports connected the carriages, moved on the guides by turning the screws (A.S. USSR №1400594, MCI And 23 To 1/12, bull. No. 21 from 06.07.1988).

However, the known device does not allow to obtain high-quality Supplement because of the poor quality of raw materials and the inability to use the materials with higher protein content. In addition, the design complexity increases the cost of the additive and complicates its use in practice.

A device for processing straw for animal feed, including two tanks with alkaline and acidic environments, and it is provided with a located between the two containers semi-permeable partition, and each tank is provided with electrodes and a source of constant voltage, opposite poles of which are connected with electrodes (A.S. USSR №1161065, MCI And 23 To 1/12, bull. No. 22 of 15.06.1985 - prototype).

However, the known device does not allow to obtain high-quality Supplement with a balanced amino acid composition, as this device does not involve the combination of different raw materials. In addition, the use of this design does not provide consolidation of technological solutions, which increases the amount of aggressive waste. Low performance design does not allow simultaneous processing of multiple types of raw materials, and therefore increases the time and cost of the resulting add the I.

Known methods and devices do not allow to obtain high-quality protein feed additive, a balanced amino acid composition, low-cost at low production time and with a small amount of aggressive waste.

Technical solution to the problem is to improve the quality and improving amino acid additive, reducing its cost, and exit of proteins due to the simultaneous extraction of various raw materials in the cells.

This object is achieved in that in the method of obtaining protein feed additives of plant materials, including processing of sunflower meal by Catolica, removing the resulting solution from the cathode side diaphragm electrolyzer, protein extraction, filtration, bringing extract to the isoelectric point of proteins, sludge drying, grinding, and processing of sunflower meal by Catolica carried out to a pH of 9-11, simultaneously treated with anolyte soybeans to a pH of 3-5 in the anode zone of the diaphragm electrolyzer, while the catholyte and the anolyte circulating at the same speed, and the extraction was carried out for 7 minutes under the action of electric current, then turn off the electric current and the extraction is carried out another 30 minutes, the obtained protein solution of sunflower meal and soybean combine.

The claimed method implements the I through the device, includes two cameras with electrolytes, United semipermeable wall, each chamber is provided with electrodes and a source of constant voltage, opposite poles of which are connected with the electrodes, and the device is equipped with two cameras for the extraction of plant materials, appropriate meal and soybean team camera and pumps and piping for circulating the working fluid connected to the chambers of the electrolyzer and the team camera for extracts.

The claimed method of obtaining protein feed additives of plant materials is part of protein-enriched raw materials that improve the quality characteristics of the concentrate, in other settings working solutions, which increases the yield of proteins due to more complete extraction and reduces the amount of toxic wastewater.

The inventive device for producing a protein feed additives of plant materials differ in other parameters of the design that enables simultaneous processing of several types of raw materials, higher performance and lower energy intensity.

These differences allow to draw a conclusion on the conformity of the proposed technical solutions to the criterion "novelty".

The features distinguishing the claimed technical solution to the prototype, aimed at achieving post is certain tasks and not revealed by the study of this and related areas of science and technology, therefore, meet the criterion of "inventive step".

The method of obtaining protein feed additives of plant materials is as follows.

The method of obtaining protein feed additives was carried out in the laboratory of the Department of biotechnology, biochemistry and Biophysics of the Kuban state agrarian University. Sunflower meal and soybean seeds were crushed in a laboratory mill "Pirouette". In the anode and cathode side diaphragm electrolyzer of ALHA-008 was filled with tap water and applied constant electric current. Due to the forced circulation both technological solution (anolyte and catholyte) processed raw material weighing 100 g of the solution of Catolica circulating through sunflower meal, and anolyte through soy flour, ensured the transition of proteins from raw materials in a soluble state. Maximum extraction was achieved when the 7-minute processing of sunflower meal by Catolica with a pH of 10, and soy flour analyte with a pH of 4. Processing of raw materials was carried out simultaneously. Next, the electric current was cut off and carried out the extraction of 30 minutes. Then the protein solutions were combined, providing a neutralization process solutions that resulted in proteins that are located in the solution to the isoelectric point, they moved into an insoluble state. The precipitated protein CTD is ranged by filtration, was dried in a drying Cabinet and was ground in a laboratory mill. The yield of protein supplements of soybean and sunflower meal 15,1%.

The obtained protein feed additive of sunflower meal and soy flour did not contain toxic compounds. The content of protein, some amino acids, as well as the amount of energy and protein yield in the proposed addition is presented in the table.

Table

The chemical composition of the additive, as well as its output and energy intensity for protein
IndexConcentration
The energy intensity for protein, kW5800
The output of the additive, %15,1
Protein, %70,1
Lysine, mg/kg2,01
Methionine + cysteine, mg/kg1,21

As can be seen from the table, the proposed addition of the protein level is high, and the cost of energy is negligible. In addition, obtained by the proposed method Supplement balanced in essential amino acids lysine and methionine due to the fact that as raw material for its preparation is used several sources. Soy contains high content of lysine and low in methionine, sunflower seeds the opposite is from. Thanks to the combination of proteins from this raw material is balancing protein on these indicators. So, derived from sunflower meal concentrate lysine amounted to only 1.3 mg/kg, which is below 0.7 mg/kg concentrate on the proposed method. In addition, unlike the prototype, upon receipt of protein supplements on the proposed method generated waste water is neutral in acidity and viscotoxin for the environment. In addition, they can be reused for extraction. The proposed technology is simplified in comparison with the prototype, as it does not require input of mineral acids, which also increases the environmentally friendly way to obtain protein Supplement.

To obtain protein feed additives of plant materials treated sunflower meal solution Catolica has a pH of 9-11. If the value of the pH is less than 9, the solubility of protein meal in the working solution will be low, which will reduce the output of the additive. If the pH value is more than 11%, the result will be a process of saponification of the residual oils and non-enzymatic oxidation of chlorogenic acid, which significantly degrades the quality of the final product. On this basis, the optimum value of pH is 10% Anolyte obtained after the treatment the processing of soybeans, should have a pH of 3-5% When the value of pH less than 3 increases the processing time and energy consumption will be overstated. Increasing the pH above 5% leads to the decrease of the solubility of the protein in the working solution. On this basis, the optimum value of pH is 4 units

The invention is illustrated in the drawing, which shows a device for implementing the method of obtaining protein feed additives of plant materials.

The device consists of two cubic container, the anode 1 and cathode 2 connected through a semi-permeable membrane 3, which is placed carbon electrodes 4, 5 and 6 to the power source, respectively. The cathode tank 1 is connected with the reaction vessel for processing of sunflower meal 7 through line 8 for circulating pump Catolica by a pump 9. Extracted by Catolica belaustegui solution passing through the filter 10, a pump 11 is supplied by pipeline 12 in the tank for neutralization and precipitation of protein 13. When this anode tank 2 is connected with the reaction vessel for processing soybeans 14 through line 15 for circulating pump anolyte by a pump 16. Extracted the anolyte belaustegui solution passing through the filter 17, a pump 18 is supplied via the pipe 19 into the tank for neutrons is the mobilization and deposition of protein 13 conical shape.

The device operates as follows.

In tanks 1 and 2 is fed tap water, and the electrodes 4 and 5, direct voltage. When this occurs the formation of solutions of Catolica and anolyte, which simultaneously through a system of pipes 8 and 15 by means of pumps 9 and 16 circulate through the tank 7 and 14, containing sunflower meal and soybean meal, respectively. In the circulation of fluids through the substrate, transition proteins in a soluble state. When the solutions necessary parameters on pH supply DC stop and stand until the end of extraction. Upon completion of the process solutions Catolica and anolyte containing dissolved protein is filtered through a filter 10 and 17. Then with the help of pumps 11 and 18 through pipelines 12 and 19 solutions are combined in the vessel 13 to neutralize the solution and deposition of protein in stocke. Then the precipitate is separated and dried.

The proposed design of the device allows simultaneous processing of multiple substrates (soybean meal and sunflower meal). When this extraction capacity, which is the dissolution of the protein that are spatially separated from the electrolysis of the device, which prevents the sticking of insoluble parts of the substrate to the electrodes and the formation of local overheating. The availability of capacity for extraction, DG who is the merger of the two solutions, containing dissolved protein, provides the neutralization of working solutions and the precipitation of the protein.

1. The method of obtaining protein feed additives of plant materials, including processing of sunflower meal by Catolica, removing the resulting solution from the cathode side diaphragm electrolyzer, protein extraction, filtration, bringing extract to the isoelectric point of proteins, sludge drying, grinding, characterized in that the processing of sunflower meal by Catolica carried out to a pH of 9-11, simultaneously treated with anolyte soybeans to a pH of 3-5 in the anode zone of the diaphragm electrolyzer, while the catholyte and the anolyte circulating at the same speed, and the extraction was carried out for 7 min under the action of electric current, then turn off the electric current and the extraction is carried out for another 30 min, the obtained protein solution of sunflower meal and soybean combine.

2. The device for implementing the method of obtaining protein feed additives of plant materials, including two cameras with electrolytes, United semipermeable wall, each chamber is provided with electrodes and a source of constant voltage, opposite poles of which are connected with the electrodes, characterized in that the device has two cameras for the extraction of plant material, respectively meal and soybeans, sborn the second chamber and pumps and piping for circulating the working fluid, connected to the chambers of the electrolyzer and the team camera for extracts.



 

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