Composition for plant treatment

FIELD: agriculture.

SUBSTANCE: claimed composition contains arachidonic acid and urea in ratio of 1:1-1:8 and additionally lower C1-C4-alkohol as solvent. More particularly composition contains (mg/1 ml of solvent): arachidonic acid 0.01-1.00 and urea 0.01-3.00. Moreover composition optionally contains ionol as antioxidant in ratio arachidonic acid/ionol of 1:1.

EFFECT: composition for plant treatment with improved effectiveness.

3 cl, 9 tbl, 7 ex

 

The invention relates to agriculture, namely crop production, and relates to compositions containing a combination of different substances that have a regulating effect on biochemical processes in plant cells, leading to the increase of drought and frost resistance of plants.

The proposed composition consists mainly of two known for its application in plant substances - urea (carbamide) and arachidonic acid (AA).

First of substances (urea) is widely used not only as a nitrogen fertilizer. A known fact about the phytotoxicity of urea itself (Agriculture abroad, 1981, No. 2, p.32) allows its use as a means of protection of plants against various diseases, such as scab of Apple and pear (Mycology and Phytopathology, 1981, t.15, issue 1, p.48-52).

In combination with other chemical compounds urea network effects, unusually for private use. Thus, an aqueous solution of urea and sodium silicate when spraying barley warns him lodging (SU 965413, a 01 N 33/04, 1981).

Arachidonic acid is less well known than urea, but it is recently widely used in agricultural and garden crops. On the one hand, it has a pronounced recostitutionalized (EN 2076598, A 01 N 25/02, 1994), and on the other causes of plant resistance to viral pathogens (EN 2072779, a 01 N 37/06, 1993) or fungal and bacterial diseases (DAN SSSR, 1989, t, No. 5, s-1265).

As in the case of urea, arachidonic acid is used in combination with other substances. So, together with a fungicide TMTD (tetramethylthiuramdisulphide) it forms a combination that increases the resistance of seedlings of sugar beet to the pathogens of root borer (SU 1605962 And 01 1/00, 1988).

Closest to the claimed is a composition for treatment of plants, increasing immunization of plants against various phytopathogens comprising as active substance arachidonic acid in a mixture of other fatty acids and urea as the target additive as a solid carrier of this mixture (EN 2075933, a 01 N 37/06, 1994). The weight percentage ratio between the mixture of fatty acids, including arachidonic acid, and urea for this song constantly and is respectively 1:13,8. If you take into account what actually arachidonic acid is only part of the mixture of fatty acids, its correlation with urea guaranteed more than 1 to 13,8.

A disadvantage of known composition is the inability to increase drought and frost resistance of plants.

The task of the invention is the creation of more effective com is osili for processing plants, with complex effects on plants.

The technical result of the claimed invention is the ability to increase drought and frost resistance of plants while maintaining the effects of increasing the yield and protection from diseases.

This technical result is achieved in that the composition for treatment of plants, comprising arachidonic acid and urea, which additionally contains an organic solvent arachidonic acid and urea, taken in a ratio of between 1:1 to 1:8, in the following ratio of ingredients, in mg/ml:

Arachidonic acid0,01-10
Urea0.01 to 80
Organic solventrest

And also the fact that as an organic solvent it contains lower alcohols with 1-4 carbon atoms.

And the fact that it further comprises an antioxidant in relation to arachidonic acid 1:1.

And as an antioxidant it contains BHT.

Figure 1 shows the table 1 - evaluation of the effect of the claimed composition on the indicator of drought tolerance in tomatoes;

Figure 2 table 2 presents data on the influence of various compositions on drought tolerance of winter wheat;

On Fig presents table 3 - the data of frost resistance of winter wheat when spraying in the tillering stage of the claimed composition, pattern, and water (control);

4 shows table 4 - effect of the claimed composition on the germination of cucumber seeds at different temperatures;

Figure 5 presents table 5 - the effect of the claimed composition on the frost resistance of plants cucumber;

Figure 6 presents table 6 - the effect of the claimed composition on the yield of potatoes;

Figure 7 presents table 7 - the effect of the claimed composition on tuber of potato diseases.

The claimed composition was prepared by serial dilution in an organic solvent, for example in 96%ethanol, urea, antioxidant and arachidonic acid in a respective weight ratio of components (the antioxidant in the amount of arachidonic acid). As the organic solvent can be used, and other lower alcohols with 1-4 carbon atoms, such as isopropyl or butyl. The presence in the composition of the antioxidant ionol (equivalent) - allows you to bind oxygen, present in a few percent of water in ethanol. Ethanol due to the very small amount of water it retains structural, and hence biologically active characteristics of arachidonic acid and mocovi the s for a long period of storage (especially with antioxidant). For both substances, the presence of water in the composition means spoilage: arachidonic acid is due to the oxidation of double bonds and urea due to hydrolysis in water to ammonia and carbon dioxide. In addition, the ethanol in comparison with many other solvents least dangerous for everyday use. In addition, the use of a fluid as a carrier of low doses (mg) of active substances is preferable solid carriers from the standpoint of simplicity and economy of their packaging in the media, as well as achieve the accuracy of this packaging.

For a better understanding of the invention the following are specific examples of its implementation.

In the manufacture of the claimed composition using arachidonic acid with a purity not less than 90% (see, for example, Reagents for biochemistry and research in the field of natural Sciences, Sigma, 2002-2003, s), urea mark 4 according to GOST 6691 and ethyl alcohol rectified 96% according to GOST 5962. Arachidonic acid is produced from marine raw materials, animal or microbial (biotechnology) origin by known techniques (see, for example, RU 2076598).

Example 1.

Trials on drought resistance of tomato varieties "New Kuban".

The working solution compositions are prepared using their cultivation with thorough stirring in pure water. When first preparing the composite dose of 1 ml on the basis of which canola and the weight hanging arachidonic acid and urea. Then these doses dissolved in 1 liter of water. As control is water, and the reference composition krezacin (0,05% working solution).

Tomato seeds in gauze bags put in cups with solutions of the claimed composition, water and krezacin and incubated for 1 hour at a temperature of 23-25°C. After 1 hour, the seeds are washed three times in water, dried and spread on Petri dishes of 40 pieces each. Pour 10 ml of water and put in a thermostat for germination at 25°C. After 72 hours conducting a first measurement of the length of the seedlings. Next cups with spouts heated at 42°C for 6 hours in thermostat. Then all of the cups again put in a thermostat at 25°and germinated for 48 hours, followed by a second measurement of the length of the sprouts.

The development of plants under normal conditions and drought resistance is determined as a percentage relative to control length of seedlings.

The results are presented in table 1, Fig.1.

As can be seen from table 1, figure 1, under normal temperature conditions the plants grow almost the same as in the investigated range of weight ratios of arachidonic acid and urea, and compared them with the reference.

However, after exposure of plants to high temperatures, the picture changes. Identified optimal from the point of view of the improvement for which acousticity range of weight ratios of arachidonic acid and urea, namely from 1:1 to 1:8, and significantly increases the difference between this range with the performance standard and the more control that indicates the high efficiency of the proposed composition for improving the drought tolerance of plants. At the same time and experienced the composition ratio of arachidonic acid to the solvent 0.005 mg/ml, which is below the claimed 0.01 mg/ml, show almost the same results as the control, i.e. it is not effective for enhancing drought tolerance.

Example 2.

Test drought tolerance of wheat varieties "MHP".

The inventive composition in an optimum range of weight ratios of arachidonic acid and urea from example 1, the composition of urea and arachidonic acid in dry form without solvent, krezacin (standard) and water (control) was used for pre-treatment of wheat seeds.

Aqueous working solutions were prepared analogously to example 1. Dry compositions on the basis of urea and arachidonic acid was prepared as follows. 1 g sample of urea was applied alcohol solution of an appropriate number of arachidonic acid. Then hinge the urea was placed in a one litre flask rotary evaporator and drove the alcohol under vacuum. Next, from the dry sub-samples were weighed using analytical balance required for experiments dose to the positions of urea and arachidonic acid.

Seeds were soaked for 30 minutes For all variants of the experience. Soaked seeds were put to germinate in Petri dishes on filter paper of 50 pieces. After three days of germination the seedlings were heated in a thermostat at a temperature of 40-42°C for 1 hour, after which germination continued.

During germination was determined by the height of shoots and length of roots of 7-day-old seedlings, dry weight of seedlings. Drought tolerance of plants is estimated by the difference of these indicators for all variants of the experience.

The results are shown in table 2, figure 2.

From the table 2, figure 2 shows that plants treated with the proposed composition of the optimal weight ratio of arachidonic acid and urea, after high temperature stress retain the intensity of growth processes is significantly higher than the plants treated in other variants, including variants of dry compositions arachidonic acid and urea in the range of weight ratios of the claimed composition. The latter figures are on the level of control and inferior to the benchmark. This fact suggests that the anti-stress effect is manifested only in compositions arachidonic acid and urea in an organic solvent.

Example 3.

The test for resistance to frost. Winter wheat cultivar "Spartano".

Dry seed is and wheat sown in trays with soil and sand of 100 pieces. Blooming plants at the age of 25 days are sprayed with aqueous solutions of the claimed composition, 0.01% krezacin as a reference and just water (control). The volume of solutions for spraying expect from field norm (400 l/ha) 30 ml per box. In 3 days after treatment the plants are subjected to hardening in the beginning within 7 days at a temperature of +2°and then one day when -5°C. Next, the plants are dug and proparaguay in the refrigerator at -10°C, -13°C, -16°and -19°C. Duration of exposure at each temperature for one day. After thawing during the day the plants are planted in boxes and 4 weeks count the number of surviving and new growth of plants. Frost estimate the percentage of the number of surviving plants from the number of plants frozen at each temperature. Aqueous working solutions of the claimed composition is prepared analogously to example 1.

The test results presented in table 3, figure 3.

The data of table 3, figure 3 shows a high-stress activity of the inventive compositions in an optimum range of weight ratios of arachidonic acid and urea, i.e., the ability to substantially increase the frost resistance of plants, including in comparison with arachidonic acid (15 mg/ml) claimed above.

Example 4.

Ispitanika frost. Cucumber is a sort of hybrid of the zodiac.

Cucumber seeds are soaked in aqueous solutions of the claimed composition, the dry composition of urea and arachidonic acid. As a reference used krezacin, and control is soaking in water. Aqueous solutions were prepared analogously to example 1. Time of seed soaking for 1 hour. We studied the germination of cucumber seeds at different temperatures.

The results are presented in table 4, figure 4.

Also studied the effect of 4-hour temperature drops to -2°With death and Tripolitania of a cucumber.

The data are shown in table 5, figure 5.

The composition of the claimed composition: arachidonic acid 0.01 mg/ml, urea 0.05 mg/ml, ethanol 1 ml of the Composition dry composition: arachidonic acid 0.01 mg, urea 0,05 mg

As can be seen from the data of tables 4 and 5, figure 4 and 5, the inventive composition is more effective in stimulating the germination of seeds under different temperature and protect them from destruction and tripolidine at low temperatures compared to the reference standard and control, and dry compositions of urea and arachidonic acid.

Example 5. A test to determine the effect of the claimed composition on yield and reducing the incidence of potato varieties "Nevsky".

Scheme of the experiment.

Two - time processing of the claimed composition (pre-tubers and obrycki is the use of vegetative plants in the budding stage).

- Two-time processing krezacin (reference).

- Dual treatment with water (control).

The tubers of the potato just before landing was treated with an aqueous solution composition comprising 0.3 mg arachidonic acid, 0.15 mg of urea in 1 ml of ethanol. The composition was dissolved in 10 l of water, which went to process 1 tonne of tubers.

Potato crops in the budding stage were sprayed with an aqueous solution of the composition at the rate of 10 mg of arachidonic acid per 1 hectare Composition for this included 10 mg of arachidonic acid and 80 mg of urea in 1 ml of ethanol. The rate of flow of aqueous solution of 400 l/ha

Krezacin (reference). Treatment of tubers (0,05%). Spraying of plants (0,004%).

Test data are shown in tables 6 and 7, 6 and 7.

As can be seen from tables 6 and 7, 6 and 7 of the proposed arrangement not only greatly increases productivity, but also reduces the incidence of fungal and bacterial diseases that says about the current composition of the known qualities of arachidonic acid and urea, as plant growth stimulators and their means of protection from various diseases.

Thus, the results of the above examples confirm the effectiveness of the aggregate components of the claimed compositions and ranges of their relationships to enhance drought tolerance and cold hardiness is of astani.

As you might expect, the effect of the claimed composition based on previously unknown ability of the composition, including arachidonic acid, urea and an organic solvent, taken in the claimed proportions to influence certain biochemical processes in plant cells, such as transformed cell water in a colloidal state, and thereby to protect the plant from various temperature stress conditions.

Example 6. The influence of composition on the germination and early growth phase of seedlings of cucumber.

The working solution compositions were prepared by dissolving weighed arachidonic acid (AA) 3 mg, urea - 9 mg of ionol 3 mg in 96%ethanol. Then by the method of dilution of the initial solution was obtained alcohol solution of the composition with the desired ratio of components in mg per 1 ml solvent: 0.01 mg AK + 0.01 mg urea + 0.01 mg of ionol; 0.03 mg AK + 0.09 mg urea + 0.03 mg of ionol, and 0.40 mg AK + 3,00 mg urea + 0,40 mg of ionol; 1.00 mg AK - 3,00 mg urea + 1.00 mg of ionol (the ratio of arachidonic acid to urea respectively 1:1, 1:3 and 1:8). In option 4, the solution contained 0.03 mg AK + 0.09 mg of urea, but without BHT. 1 ml of the alcohol solution was diluted in 1 l of water, getting a working solution. The benchmark was the drug El-1. In the working solution and soaked the seeds of cucumber variety Nightingale for 30 minutes, then were germinated and the settlement of the e-counting germinated seeds were grown seedlings up to 14 days of age under optimal conditions. The experimental data presented in table 8, Fig. As can be seen from the data, the composition stimulated the germination of cucumber, increasing the germination rate by 25% in comparison with the control. The composition was more effective than standard. Under the action of the composition increased stem diameter of seedlings (41-42%), was significantly increased wet and dry weight (3 times more than in the reference version).

Example 7. The effect of the claimed composition on growth, development, yield and resistance of potato cultivars and Santa to diseases.

Potatoes early and medium varieties of Santa (Agrico, the Netherlands) were grown in common for the Central non-Chernozem zone management.

Seed tubers after treatment were planted in pre-cut ridges scheme 70×35 cm planting Depth of 8-10 cm, the rate of planting 2.6 t/ha planting density of tubers based 41 thousand units per 1 ha Total area of field experimental plots: 50 m2the repetition of four -, hosted - indonesiafree. Preparation of the solution of the preparation described in example 6, except option 3 BHT.

The scheme of experiment:

1. The control without treatment

2. The benchmark. El-1. Treatment of tubers before planting, the fertilizer consumption rate of 1.4 ml/t, the flow rate of the working solution of 10 l/t; double spraying: 1st phase full shoots, 2nd in the phase of budding and early flowering, the consumption of a sterile 10 ml/ha, the flow of work is his solution of 300 l/ha

3. A mixture of arachidonic acid + urea + BHT at the ratios of arachidonic acid to urea of 1:1, 1:3 and 1:8 (see table 9, figure 9). Treatment of tubers before planting, consumption of the drug 10 ml/t, the flow rate of the working solution of 10 l/t; spraying of plants in the budding stage, consumption of the drug 300 ml/ha solution consumption 300 l/ha

1. Composition for improving drought - and frost-resistant plants while maintaining the effects of increasing the yield and protection from diseases, including arachidonic acid and urea, characterized in that it further comprises an organic solvent is a lower alcohol with 1 to 4 carbon atoms, arachidonic acid and urea taken in the ratio of from 1:1 to 1:8, in the following ratio of ingredients, in mg per 1 ml of organic solvent:

Arachidonic acid0,01-1,00
Ureafrom 0.01 to 3.00

2. The composition according to claim 1, characterized in that it further comprises an antioxidant in relation to arachidonic acid 1:1.

3. The composition according to claim 2, characterized in that as an antioxidant it contains BHT.



 

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16 cl, 1 tbl, 4 ex

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2 cl, 2 tbl, 1 ex

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