Egg treatment method (versions)

FIELD: poultry farming.

SUBSTANCE: method involves withdrawing material from multiplicity of live eggs; analyzing withdrawn material for identifying of eggs having predetermined characteristic; treating in proper manner.

EFFECT: increased efficiency by providing the possibility of analyzing withdrawn material and performing proper treatment.

58 cl, 54 dwg

 

The present invention relates to methods and devices for processing eggs.

Establishing the differences between the eggs of poultry ("eggs") on the basis of some notable quality is well known and has long been used in practice in poultry production on an industrial basis. "Ovasapyan" (check end-to-end probing) is a conventional name for one such technical reception, this term has its roots in the original validation of the egg with light from a candle. Although eggshell seems opaque under most lighting conditions, in fact the eggs a little Shine. Accordingly, if the egg is placed before the light, you can see its contents.

Here are the shops poultry one purpose of Boscoreale eggs is the recognition and then the Department of live eggs (i.e. eggs from which you want to display in the incubator live poultry) from inanimate eggs (e.g., unfertilized eggs, dead eggs, rotten eggs, empty eggs etc). In U.S. patent No. 4955728 and 4914672 described device for Boscoreale that uses infrared detectors and infrared radiation emitted from the egg, to identify live eggs. In U.S. patent No. 4671652 described device for Boscoreale, in which multiple source the light and the respective photodetectors are installed in the matrix and in which to identify live eggs eggs pass between the light sources and photodetectors.

After identification of the live bird eggs can be treated with drugs, nutrients, hormones and/or other beneficial substances, while the embryos are still in the egg (i.e. in ovo). Introduction in ovo various substances in avian eggs are used with the aim of reducing morbidity and mortality after hatching, increasing the potential growth rate or the possible magnitude that is growing in the birds and even the influence on sex determination of the embryo. The introduction of vaccines in live eggs effectively used for immunization of birds in ovo. In poultry production on an industrial basis additionally, it is desirable to manipulate the embryo in ovo to enter in the developing embryo of a foreign nucleic acid molecule (i.e. create a bird with transgenic mutation) or to enter foreign cells (i.e. create a chimeric bird).

The introduction of the virus in ovo can be used for reproduction of a specific virus, intended for use in the preparation of vaccines. Examples of substances that are used or proposed for introduction in ovo include vaccines, antibiotics and vitamins. Examples of therapeutic agents for administration in ovo and ways of administration in ovo described in U.S. patent No. 4458630 and U.S. patent No. 5028421.

Improved methods of injection of eggs containing an embryo, you can use the ü for sampling eggs, including embryonic and Nazaraliev substances. In addition, other applications may need to enter the sensor inside the egg containing an embryo to obtain therefrom information, for example as described in U.S. patent No. 6244214.

In industrial incubator workshops during the incubation, the eggs are usually contained in the pads for laying eggs. At the chosen moment of time, usually on the eighteenth day of incubation, eggs are removed from the incubator. Unsuitable eggs (namely dead eggs, rotten eggs, empty and unfertilized eggs, identify, and remove, live eggs treated (for example, are vaccinated) and then transferred into containers for incubation.

When managing an incubator workshops may be desirable to divide the birds on the basis of various characteristics, such as gender, disease, genetic characteristics, etc. for Example, may need to be vaccinated birds the males of a particular vaccine, and birds to be vaccinated females other vaccines. The sexual division of birds during insertion may be important also for other reasons. For example, turkeys usually divided by gender due to the difference in the rate of growth and the nutritional needs of male and female turkeys. In the production of hens or table eggs it is desirable to keep only females. In the production of broilers is desirable to separate the birds on the basis of polads win in the efficiency of feed utilization, improve the uniformity of processing and reduce production costs.

Unfortunately, conventional methods of determining the sex of birds can be expensive, laborious, time consuming and usually require trained people with specialized skills. Conventional methods of determining the sex of birds include sex determination feathers, kloachnye method of sex determination and the establishment of gender by DNA or blood. Paul feathers, you can determine approximately three thousand (3000) chickens per hour, at a cost of approximately 0.7 to 2.5 cents per chick. Kloachnye method of sex determination, you can determine the gender of approximately fifteen hundred (1500) chickens per hour, at a cost of approximately 3.6 to 4.8 cents per chick. Establishing a floor on DNA or blood performed by analyzing a small sample of blood taken from birds.

It may be desirable to identify the sex of the birds, as well as other characteristics of birds to breeding. Sex identification for breeding can significantly reduce costs for the various partners in farming on an industrial basis. Although using conventional methods Boscoreale can to some extent effectively distinguish between viable and non-viable eggs, these conventional methods Boscoreale unable to reliably determine the sex and other characteristics out unhatched birds.

PR is accepting of the foregoing, embodiments of the present invention provide methods of processing eggs that have identifiable characteristics (e.g., gender), in which the material (for example, allantoin fluid, amnion, yolk, shell, fiber, fabric embryo Solopova shell and/or blood etc) is extracted from each of the set of live eggs, the excavated material is analyzed in order to identify eggs having some characteristic, and then the eggs identified as having this characteristic, processes accordingly. For example, a method of processing eggs based on the field, in accordance with the variants of the implementation of thethe present invention includes identifying live eggs among many eggs by extracting allantoine fluid from the eggs identified as live eggs, detection of the presence of estrogenic composition in allantoine fluid extracted from each of the living eggs, to identify each floor of the living eggs, detection of color change allantoine fluid to identify the sex of each egg and then selectively introducing a vaccine into live eggs in accordance with the floor.

In accordance with the variants of implementation of the present invention removing allantoine fluid from eggs includes the location of each of the live eggs, essentially, in izontally orientation, therefore the allantois of each egg is forced to unite and increase allantoine bag under the top part of the shell of each egg, the introduction of a probe (e.g., needles) in each egg through the shell of the egg and directly into the enlarged allantoine bag and removing the sample allantoine liquid from the allantois of each egg through each probe. In accordance with the variants of implementation of the present invention the detection of the presence of estrogenic composition in allantoine fluid includes distribution allantoine liquid, extracted from living eggs into appropriate vessels and the distribution of the biosensor in vessels where the biosensor is configured to chemical reaction with estrogenic composition in allantoine fluid and change the color allantoine liquid.

In accordance with the variants of implementation of the present invention the selective introduction of the vaccine in living eggs in accordance with the floor includes the introduction of the second vaccine live eggs identified as males, and the introduction of the second vaccine live eggs identified as females. Alternatively, the selective introduction of the vaccine in living eggs in accordance with the floor includes the introduction of vaccines in live eggs identified as having the same gender.

In accordance with other options to implement the Oia of the present invention the material, retrieved from eggs can be analysed with the aim of identifying one or more pathogens inside each egg. Eggs identified as having one or more pathogens, subsequently removed from the rest of the living eggs.

In accordance with other variants of implementation of the present invention on the material extracted from eggs, it is possible to conduct genetic research.

In accordance with the variants of implementation of the present invention is provided an automated sorting system on the floor, which consists of three independent modules, connected through a network. The first module is a module sampling allantoine fluid. Strip to remove eggs from the incubator eggs, usually at 15, 16 or 17 day 21 day incubation cycle, and is served on a conveyor belt. Optical sensor automatically identifies live eggs, and eggs (or just live eggs, all eggs) are transferred into the matrix nests for eggs. Each nest for eggs configured to change the position of the respective eggs in its lateral position and place the egg in the center. Then in each egg is injected the needle to a depth of approximately five to six millimeters (5-6 mm) at the place near the midpoint of the eggs, and extracted allatoona liquid (for example, when listello 20 µl). The sample fluid from each egg is placed in the appropriate cell is equipped with a bar code template for analysis. The cells in the template can be located in the same periodic structure as the matrix (periodic structure) gaskets for eggs, in accordance with the variants of implementation of the present invention. Each needle for sampling subjected sanitized before use to sample material from another egg.

The position of the eggs change through nests for eggs in the vertical position, and then return in with a bar code strip for eggs. After laying the egg is usually returned to the incubator eggs. Templates for analysis containing a selected material (e.g., allantoin liquid) from eggs laid for processing, and a processor for processing data in the network verifies the bar codes of each strip for eggs and template for analysis.

The second module is an automated module analysis. The operator loads a set of templates for analysis, contains selected material from eggs (for example, allantoin liquid) in the module analysis. Inside the module analysis each template for the analysis moves through the conveyor system under distribution head, which distributes in advance is definitely the certain number, for example, approximately 75 μl of reagent (for example, marking biosensor cellular basis LiveSensors™, LifeSensors, Inc., Malvern, PA (Pennsylvania) in each corresponding cell. Then each template for the analysis progresses through the chamber with a controlled environment within a predetermined period of time (for example, approximately 3.5 hours). Each template for the analysis moves through the conveyor system under another distribution head which dispenses a predetermined quantity of coloring substrate (e.g. substrate on the basis of ONPG-O-nitrophenyl-beta-D-galactopyranoside) in each cell. After that, each template for the analysis progresses through the chamber with a controlled artificial climate within a predetermined period of time (for example, approximately 45 minutes)to provide the opportunity to show the color inside each cell.

Then the camera on the CCD (charge-coupled devices) scans each cell to determine the sex of the relevant eggs, selected material which is in the cell. This information is stored using a data processor in the network. In accordance with the variants of implementation of the present invention after the reagent (for example, marking biosensor cellular basis LiveSensors™) inside each of the second cell is destroyed (for example, using heat and/or chemical treatment) to remove each template for analysis.

The third module is a module for processing and sorting eggs. In accordance with the variants of implementation of the present invention is equipped with a bar code strip to remove eggs from the incubator the eggs towards the end of the 21-day incubation cycle (for example, 18 or 19 day etc) and placed on a conveyor system. In accordance with the variants of implementation of the present invention, the processor processing data in the network determines which eggs are males and which are females, on the basis of pre-stored information. Then eggs males Vaccinium vaccine selected for males and eggs in females Vaccinium vaccine selected for females. In accordance with the variants of implementation of the present invention for eggs of males and females you can use a separate device vaccination. After vaccination eggs are sorted by sex and transferred to containers for incubation of eggs of a certain sex. After that, the containers for incubation moved into incubators for hatching. In accordance with the variants of implementation of the present invention, the eggs of the same sex may be rejected and not vaccinated or transferred into containers for incubation.

In accordance with options assests the of the present invention, the eggs are first divided by gender (or other characteristic), and then treated. For example, eggs can be sorted by sex, and then the eggs of males and females be treated separately.

In accordance with the variants of implementation of the present invention are identified estrogenic compounds present in allantoine fluid embryos in females, but not present in embryos males. Avian embryos can be sorted by sex on the basis of the presence of estrogenic compounds in allantoine fluid embryos of females between the thirteenth and eighteenth days (13-18) incubation in embryos of broiler producers of broilers, turkeys and laying hens and regardless of age or species groups of animals.

Embodiments of the present invention can increase production efficiency, saving space incubation (for example, not bringing chickens identified before excretion as males), saving at vaccination, providing the possibility of reducing the share of physical labor and increasing the processing speed of incubator workshop. For example, using embodiments of the present invention when sorting by sex and vaccination can be achieved productivity between about twenty thousand and thirty thousand (20000 to 30,000 eggs per hour and with a degree of accuracy greater than ninety-eight percent (98%). P the floor because eggs are known prior to vaccination, can be obtained savings in the cost of vaccination, particularly when it is desirable to vaccinate only concrete floor. In addition, embodiments of the present invention can easily be performed even by unskilled workers.

Below the invention will be described in more detail with the involvement of the drawings, where:

figure 1 is a graphical chart of the operations for processing eggs in accordance with the variants of implementation of the present invention;

figure 2 is a graphical chart of the operations for identifying live eggs among many eggs in accordance with the variants of implementation of the present invention;

figure 3 is a graphical chart of the operations for Boscoreale eggs in accordance with the variants of implementation of the present invention;

figure 4 is a graphical chart of the operations for the spectral Boscoreale eggs in accordance with the variants of implementation of the present invention;

figure 5 illustrates the approximate spectra for three eggs subjected to the operations of spectral Boscoreale in figure 4;

6 is a graphical chart of the operations for light and heat Boscoreale eggs in accordance with the variants of implementation of the present invention;

7 is a graphical chart of the operations for extracting material from eggs in accordance with Varian the AMI implementation of the present invention;

Fig is a schematic illustration of the eggs in an inclined orientation and illustrating the Association of the allantois under the upper shell eggs;

figure 9 is a graphical chart of the operations for analyzing the extracted material eggs with the aim of identifying the characteristics of eggs in accordance with the variants of implementation of the present invention;

figa-10B are flowcharts of operations for selective processing of eggs on the basis of the identified characteristics in accordance with the variants of implementation of the present invention;

11 is a block diagram of systems and methods for processing eggs in accordance with the variants of implementation of the present invention;

Fig is a schematic illustration of the architecture of the tools top-level management system for processing eggs in accordance with the variants of implementation of the present invention inside an incubator workshop, in which control point extraction material, item analysis, items processing and sorting eggs, respectively, are separate PLCs (programmable logic controllers);

figa-13D represent a more detailed illustration of the architecture of the tools top-level management system for processing eggs in accordance with the variants of the implementation of this breath is retene inside an incubator workshop, in which control point extraction material (module sampling), module analysis module and move, respectively, are separate PLC;

Fig is a side view in vertical section of a device for the extraction of material (also referred to as a module sampling) from a variety of eggs in accordance with the variants of implementation of the present invention;

Fig represents an enlarged view of the device for the extraction of material on Fig illustrating the moving device and the two devices sampling on opposite sides of the device, movement;

Fig is a top view of conveyor systems gaskets for eggs and nests for eggs devices retrieve material on Fig, taken along the lines 16-16;

Fig is a side view in vertical section of the device of the extraction material Fig illustrating the lateral movement device moving the eggs between the two conveyor systems gaskets for eggs and nests for eggs;

figa illustrates loading of incoming strips for eggs on the incoming conveyor system gaskets for eggs and loading of empty pads for eggs on the output conveyor system gaskets for eggs. Figa also illustrates the incoming strip for eggs, installed inside the field Ovasapyan the I device extraction material Fig;

figv illustrates the movement of incoming strips for eggs on the incoming conveyor system gaskets for the eggs to the area of a gripping device, where the device is moving eggs egg moves from the incoming strips for eggs in nests for eggs;

figs illustrates many eggs placed inside the set nests for eggs after moving from the incoming strip for eggs through the device transferring the eggs;

fig.18D illustrates the movement of nests for the eggs to the location where the configured sampling device for extracting material from eggs that are installed inside the nests for eggs;

Fig is a perspective view of part of the matrix nests for eggs, configured to receive eggs, essentially in a vertical orientation and movement of eggs, essentially in a horizontal orientation in accordance with the variants of implementation of the present invention;

Fig represents an enlarged perspective view of the nests for eggs in the matrix Fig;

Fig is a top view of the nest for eggs pig, taken along the lines 21-21;

Fig is a side view in vertical section of a nest for eggs pig, taken along the lines 22-22;

Fig is a side view of the device of the location of eggs in accordance with alternative options for implementation of the present invention, p is item egg, essentially in a horizontal position;

Fig illustrates the device location eggs pig, in which: egg turn, essentially in a vertical orientation, with an element for orientation;

Fig is a partial top view of the device layout eggs pig, taken along lines 25-25 and illustrating the inclined upper ends of the first and second areas;

Fig is a partial end view of the device layout eggs pig, taken along lines 26-26;

Fig is a top view of the lifting head of the unit move the eggs Fig illustrating a matrix manifold blocks and suction cups, in which the matrix is in the extended configuration;

Fig is a top view of the lifting head pig and on which the matrix manifold blocks and the suction cups is compressed in the first direction;

Fig is a side view in vertical section of the lifting head pig, taken along lines 29-29;

Fig represents an enlarged side view of one of the flexible suction lifting head pig, which is configured to move the eggs in accordance with the variants of implementation of the present invention;

Fig is a side view of the head sampling to extract material from the egg in accordance with variants of the OS the implement of the present invention;

Fig is a side view in section of a nest for the eggs inside illustrated by Fig matrix with egg, installed it, essentially in a horizontal position, and illustrating the head sampling in contacting relation with egg;

Fig is a side view of the many heads of sampling for one of the four sampling devices for Fig, in which each cylinder of the sample is in contact with the shell of the egg within the nest for eggs to extract material from the egg, and on which the needle sampling within each head sample is in the retracted;

Fig illustrates head sampling on Fig where the needle sampling are in the first extended position, pierce the shell of each respective eggs and are in position to extract material from each respective egg;

Fig illustrates head sampling on Fig, in which needle sampling are in the second extended position for placement of the material extracted from the relevant eggs into appropriate vessels for samples in the template for analysis;

figa illustrates one of the heads of sampling on Fig with item bias, illustrated by dashed line;

figv illustrates the head taking the specimen is in figa, in which the power of the air displacement in the lower half of the cylinder head sampling overcome so that the needle sampling is in the first extended position, pierced the shell of the egg and is set to extract the material from the egg;

figs illustrates the cylinder sampling on FIGU, where the power offset bias element is overcome so that the needle sampling is in the second extended position and prepared for placement of the material extracted from the eggs in the vessel for samples and subsequent sanitation;

fig.36D illustrates an example of disinfectant fountain, which can be used for sterilization of the corresponding needle sampling in accordance with the variants of implementation of the present invention;

Fig is a top view of a matrix of heads of sampling on Fig, taken along the lines 37-37 and illustrating the locking plate in accordance with the variants of implementation of the present invention, which is configured to maintain each head sampling in the vertical locked position relative to the corresponding eggs when removing material from the egg;

figa is a top view of the locking plates on Fig in accordance with one embodiment of the present invention;

figv p is ecstasy top view of the locking plates in accordance with an alternative embodiment of the present invention;

figa is a side view of the head sample of the matrix Fig illustrating the blocking plate in the linked position relative to the head sampling;

figv illustrates the cylinder sampling on figa, in which the locking plate is moved to the right and engages with the head of the sampling, in order to pin down the head of the sampling to the two stationary plates;

figs illustrates the cylinder sampling on figa, in which the blocking plate attaches to the head of the sampling to the two stationary plates so that the vertical movement of the head sample is limited;

Fig is a top view of the tray of samples, with multiple vessels for samples, configured to receive material to be extracted from the eggs, in accordance with the variants of implementation of the present invention;

Fig represents an enlarged partial top view of the tray of samples on Fig illustrating the material extracted from eggs placed within the respective receptacles of the sample tray of samples;

figa-42V represent the top of the system processing trays for samples in accordance with the variants of implementation of the present invention and illustrating the sample trays that are moved relative to the device taking Bristow on Fig;

Fig-44 are block diagrams of systems and methods for analyzing material extracted from a variety of eggs, to identify eggs having one or more features in accordance with the variants of implementation of the present invention;

Fig is a top view of the tray for samples in which each vessel was analyzed material eggs to detect the visible display characteristics of the respective egg;

Fig is a side view in vertical section of the device analysis for analysis of material extracted from the eggs contained within a set of trays for samples, in accordance with the variants of implementation of the present invention;

Fig is a side view in vertical section of the sorting device in accordance with the variants of implementation of the present invention;

Fig is a top view of a sorting device according Fig, taken along the lines 48-48;

Fig is a top view of the device bookmarks and injection, which is intended for use in connection with the sorting device according to Fig in accordance with the variants of implementation of the present invention;

Fig is a top view of the device of the bookmark, which is intended for use in connection with the sorting of ustroistvom Fig and processing device in accordance with the variants of implementation of the present invention;

Fig is a side view in vertical section of the device of bookmarks on Fig;

Fig is a perspective view of item processing and sorting in accordance with other variants of implementation of the present invention;

Fig is a top view of conveyor systems gaskets for eggs and nests for eggs devices retrieve material on Fig, taken along the lines 16-16, which includes the unit of analysis for analyzing material extracted from a variety of eggs, in accordance with the variants of implementation of the present invention;

Fig is a schematic representation of the device analysis Fig.

Below the present invention will be charged more fully with reference to the accompanying drawings showing preferred embodiments of the invention. However, this invention can be realized in many different forms and should not be construed as limited here formulated options for implementation; rather, these embodiments of chosen so that this disclosure is thorough and complete and fully convey the specialists in this field of technology scope of the invention. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as is usually ponimaesh the specialists in the field of technology to which this invention belongs. The terminology used here in the description of the invention presented only for the purpose of describing particular embodiments only and is not intended to limit the invention.

In the description of the invention and the attached claims forms in the singular are intended to include plural forms unless the context clearly indicates otherwise.

All mentioned publications, applications, patents and other background information are given here by reference.

The terms "bird" and "bird", as used here, include males or females of any avian species, but primarily intended to cover poultry, which are commercially bred to obtain eggs or meat. Accordingly, the terms "bird" and "bird", in particular, are intended to cover chickens, turkeys, ducks, geese, quails and pheasants. The term "in ovo (in the egg), as used here, refers to birds that are contained inside the egg before hatching. The present invention can be implemented with any type of eggs of birds, including, but not limited to, eggs, chickens, turkeys, ducks, geese, quails and pheasants.

Used herein, the term "injecting" and "injection" embrace the ways of introduction of the device (about the a rule of the elongated device in the egg or embryo, including how the release or discharge of a substance into an egg or embryo, methods of removal of substance (i.e. sample) from an egg or embryo and/or ways of introducing the device detector in the egg or embryo.

Used herein, the term "allatoona fluid" encompasses allantoin fluid with presence of other materials, eggs, or without them. For example, the term "allatoona liquid" may include a mixture of blood and allantoine liquid.

The term "predetermined location", as used here, indicates the fixed position or depth inside the egg. For example, the device may be introduced into the egg to set the depth and/or at a set place in the egg. In alternative embodiments, the implementation of the introduction can be performed on the basis of information obtained from eggs, for example, in relation to the position of the embryo or subliminally cavity inside the egg.

Methods and apparatus in accordance with the variants of implementation of the present invention can be used to identify one or more characteristics of the egg at any time during its period of embryonic development (also referred to as the incubation period). Embodiments of the present invention is not limited to a particular day during the period of embryonic development.

Figure 1 illustrates the HVL of the ordinary processing of live eggs, based on the identified characteristics, in accordance with the variants of implementation of the present invention. Initially identifies live eggs among many eggs under incubation (block 1000). For example, the eggs will otoscopic to determine which eggs are real eggs. From every living eggs extracted material (block 2000), and the extracted material is analyzed to determine one or more characteristics: for example, gender, faecal pathogens, genetic markers associated with the health of birds or productivity, nutritional, endocrine or immune indicators or factors, etc. of the respective eggs (block 3000). Live eggs are selectively processed based on the identified one or more characteristics (4000 block). Each of these operations are described in detail below.

Figure 2 identifying live eggs among many eggs (block 1000) can contain a variety of techniques, including, but not limited to, conventional Ovasapyan (block 1100), the spectral Ovasapyan (block 1200) and a combination of light and heat Boscoreale (block 1300). Embodiments of the present invention can use any method of determining whether an egg is a living embryo, and is not limited ways described here.

Figure 3 on Ichnya techniques Boscoreale include measurement of the opacity of eggs for visible light, infrared radiation and/or other electromagnetic radiation (block 1110) and then identifying live eggs, using the measured values of opacity (block 1120). Exemplary methods and apparatus of Boscoreale described in U.S. patent No. 4955728, 4914672 and U.S. patent No. 4671652, referred to here as a reference. Ordinary methods of Boscoreale should be understandable to experts in the art and need not be described here.

Figure 4 the spectral Ovasapyan (block 1200) includes the irradiation of eggs light at wavelengths in the visible and in the infrared range unit (1210) and then receiving light passing through the egg, the detector is placed next to the egg (block 1220). For example, an egg can be irradiated with light at wavelengths of from about three hundred nanometers to approximately one thousand one hundred nanometers (300 - 1100 nm). The intensity of the received light for eggs is determined at selected wavelengths (block 1230), and the generated spectrum, which displays the intensity of light at wavelengths of visible and infrared ranges (block 1240). Then generated for the eggs spectrum is compared to a spectrum associated with a live egg to determine whether an egg is a living egg (block 1250).

Figure 5 shows the three spectrum for the three relevant eggs, voskoboinik with the aid of the completion techniques spectral Boscoreale. Wavelength in nanometers (nm) is plotted on the X-axis, and the unit of light intensity plotted on the y-axis. Spectrum 2 is associated with the unfertilized egg. Range 3 is associated with prematurely dead egg. Range 4 is connected with a live egg. The spectral Ovasapyan described in application for U.S. patent No. 09/742167 registered 20 December 2000

Figure 6 light and thermal Ovasapyan (block 1300) includes the measurement of the opacity of eggs (block 1310), the temperature measurement eggs (block 1320), and using the measured values of opacity and temperature to determine whether an egg is a living egg (block 1330). Light and thermal Ovasapyan described in application for U.S. patent No. 09/563218, registered on may 2, 2000

With reference to Fig.7 below will be described the operations for the extraction of material from living eggs (block 2000) in accordance with the variants of implementation of the present invention. A lot of live eggs are essentially in a horizontal orientation so that the allantois of each egg inevitably merged inside allantoinase bag under the top section of the shell of each egg (block 2100). The term "essentially horizontal orientation", as used here, means that the egg is located so that its longitudinal axis is oriented at an angle between approximately ten degrees (10°) and bring the flax hundred eighty degrees (180° ) relative to the vertical, where the vertical angle of zero degrees (0°) is defined by a wide end of the egg as directed vertically upward position. The probe (for example, needle etc) is injected into each egg through the shell of the egg and directly into allantoin bag under the top section of the shell of the egg (block 2200). Fig illustrates the Association of the allantois 16 in eggs 1 at the top side of the egg due to non-vertical orientation of the eggs (e.g., long axis And oriented at an angle between approximately 10° and approximately 180°).

As should be known to experts in the art, during the final stages of incubation, the allantois usually exists as a relatively thin layer under the internal skorlupoy shell eggs and essentially surrounds where the embryo. At a later stage eggs with developing embryos (in the third and fourth quarters), the allantois can be difficult for accurate insertion of the needle or probe. In accordance with the variants of implementation of the present invention, the eggs are oriented essentially horizontally so that the allantois could reliably be targeted in ovo. By changing the position of the eggs, essentially in a horizontal orientation, the availability of the allantois grows. See, for example, U.S. patent No. 6176199 and U.S. patent No. 5699751, referred to here as the level of technology.

As should be clear to experts in the art, the size of the allantois is associated with the stage of embryonic development of eggs, subject to injecting; thus, the depth of insertion necessary to achieve the allantois may vary depending on the stage of development of the eggs, as well as species and varieties used bird eggs. Depth of insertion should be large enough to accommodate the sampling device within the allantois, but not large enough to pierce the amnion or embryonic. In accordance with the variants of implementation of the present invention using a needle with a blunt tip can help to minimize the possibility of puncture of the amnion or embryonic.

The exact location and angle of insertion of the sampling device inside the egg is a matter of choice and may be in any area of the eggs. The orientation of the device sampling will depend on the orientation of the eggs, the equipment available to extract the material, and the purpose of extraction of the material.

Embodiments of the present invention is not limited to removing material from the allantois or from areas near the upper surface of the eggs. Removing material from the allantois, as described here, offered just as one example of a possible variant is now implementation of the present invention. Embodiments of the present invention is not limited to removing allantoine fluid. From the egg, you can extract and analyze a variety of materials (e.g., amnion, yolk, shell, fiber, fabric embryo Solopova shell and/or blood etc) to identify one or more characteristics as described below. In addition, before removing the material from the eggs do not need to refocus essentially in a horizontal position. The material can be extracted from eggs with virtually any orientation.

From Fig.7, it is clear that the sample allantoine fluid is extracted from the allantois of each egg (block 2300). Then eggs reorienting essentially in a vertical position for more convenient manipulation (block 2400) and move to another location for further processing (block 2500).

Figure 9 describes the operation for analyzing the material extracted from each of the living eggs, to determine one or more characteristics of eggs, such as gender (block 3000) in accordance with the variants of implementation of the present invention. Material type allantoine fluid extracted from each egg, distribute to the appropriate vessels for samples in the template (block 3100). The sensor, which is configured for chemical interaction with the material of the egg and the formation of p is daumas detection signals (for example, electromagnetic signals, fluorescent signals, fluorescent signals, signals conductivity, colorimetric signals, pH-signals (signals pH) and so on), distribute to the appropriate vessels for samples (3200 block). In each of the relevant vessel is possible to add a coloring substrate, for example, the substrate on the basis of ONPG), which is configured to change the color of the material in response to a chemical reaction between the material of the eggs and biosensor (block 3300).

Then determined by the presence of characteristic eggs (block 3400). For example, the color change may indicate that in allantoine liquid in an appropriate vessel for the samples of the present estrogenic compounds, thereby indicating the floor of the respective eggs, from which was made to sample allantoine fluid. The operation represented by block 3400, are designed to enable the detection of electromagnetic signals produced inside the vessels for samples that provide an indication of the presence of the characteristic eggs. In accordance with other variants of implementation of the present invention the operations represented by block 3400, are designed to enable the detection of pathogens in the material of the eggs.

On the material of the eggs in containers for samples, you can perform one or more of Ni is sustained fashion analysis (section 3500). For example, the material can conduct genetic analysis.

On figa-10B shows the operation for selective processing of live eggs on the basis of the identified characteristics (4000 block) in accordance with the variants of implementation of the present invention. Not the basis of the identified characteristics of each egg you can enter in ovo one or more substances (unit 4100). For example, in accordance with the floor eggs can be entered into the eggs of the vaccine. In addition, eggs identified as males, you can enter the first vaccine, and eggs identified as females, you can enter the second vaccine. In addition, in accordance with the identified characteristics of live eggs can be sorted (block 4200). For example, if the identified feature is a floor, the eggs of males can be separated from the eggs of females.

Sorting can be performed before, after or instead of injection in ovo or other process or treatment. As illustrated in figv, the operations of blocks 4100 and 4200 on figa can be performed in reverse order. For example, the first eggs can be sorted in accordance with the floor, and then, on the basis of gender, to be injected by one or more substances (e.g., males can be vaccinated with one substance, and females can be vaccinated different substance and/or at another time).

Figure 11 shows with the relevant options for the implementation of the present invention, the system 10 processing of eggs, designed for handling eggs. Illustrated system includes a sorting machine 12, which is configured to identify live eggs among many eggs 1 coming in laying 5 eggs. The sorting machine 12 operatively connected with the control device 20 that controls the sorting machine 12 and stores information about each egg 1 (for example, whether the egg is alive, infertile eggs, dead, rotten and so on). As described above, the sorting machine 12 may include a system of ordinary Boscoreale, the system spectral Boscoreale, Boscoreale, which uses a combination of light and heat Boscoreale, or any other device/technique to identify live eggs and/or dead eggs, unfertilized eggs, rotten eggs, and so on). In order to provide an opportunity for the operator to interact with the managing device 20, preferably provided by the device 22 for the operator with the system (e.g., display).

Paragraph 30 of the extraction material (also referred to as a module sampling), paragraph 40 handling eggs and paragraph 50 sorting eggs installed downstream relative to the sorting machine 12 and each operatively connected with a control device 20. Paragraph 60 of the analysis quickly is outinen with the managing device 20. Paragraph 30 of the extraction material is configured to extract material, type allantoine liquid selected from eggs. The material extracted from each eggs, is analyzed using 60 analysis with the aim of identifying one or more characteristics of each of the eggs or for diagnostic or other purposes. For example, by analyzing extracted from the egg material may be identified sex of each egg. Alternatively, it is possible to detect the presence of pathogens and/or the removed material can be various genetic studies.

Paragraph 40 of the handle is formed for processing the selected eggs, for example, by inoculation substance processing (e.g., vaccines, nutrients etc). Paragraph 40 of the processing may include at least one reservoir 42 for content processing substances, be inserted in the selected eggs. The control unit 20 produces a signal selective processing for eggs (or group of eggs) on the basis of the characteristics of eggs (or group of eggs), identified by paragraph 60 of the analysis. For example, eggs, identified as females, can be injected specific vaccine with 40 processing after receiving the signal processing of upravlyaushih the device 20.

Paragraph 50 of the sort generated for sorting eggs on the basis of the identified characteristics. The control unit 20 produces a signal selective sorting for eggs (or groups of eggs on the basis of the characteristics of eggs (or group of eggs) identified by paragraph 60 of the analysis. For example, eggs, identified as males, can be placed in the first compartment for removing and eggs identified as females, can be placed in the second compartment for removal.

Paragraph 60 of the analysis formed to perform various tests on the material to be extracted from the eggs, with the aim of identifying one or more characteristics (e.g., gender) of each egg. With paragraph 60 of analysis you can perform various tests. The present invention is not limited to identification of the sex of the eggs.

The controller 20 preferably includes a processor or other suitable programmable or non-programmable circuit, including the appropriate software. The control device 20 may also include other devices that are suitable for the control point 30 of the extraction material, paragraph 40 handling eggs, paragraph 50 sorting eggs and paragraph 60 of the analysis. Suitable devices, circuits and software for the implementation of Council the managing device 20 will be obvious to experts in the art upon reading the above and subsequent descriptions and descriptions of U.S. patent No. 5745228 and 4955728.

The device 22 operator system may be any appropriate user connection device and preferably includes a touch screen and/or a keyboard. The device 22 for the operator with the system may provide an opportunity for the user to retrieve various information from the control device 20, to set the various parameters and/or to program/reprogram the controller 20. The device 22 for the operator with the system may include other peripheral devices such as a printer, and connect to the computer network.

In accordance with alternative ways of implementing the present invention one or more points of the system described relative to 11, can be controlled using individual programmable logic controllers (PLC). Data can be mutually transferred from the PLC to the control device database of the Central computer for storage and back. For example, it is possible to provide a Central database for storing information such as gender (as well as other identifiable characteristics) of processed eggs. Control device database of the Central computer is arranged to respond to individual PLCs when they request data or send data. For a database d is antralnogo computer is not required to directly manage the various items under the control of the respective PLC.

On Fig presents an illustration of the architecture management tools top-level variant of implementation of the present invention inside an incubator workshop, which uses individual PLC to control various points incubator workshop in accordance with the variants of implementation of the present invention. In the illustrated embodiment, the multiple PLC 70A, 70b, 70C control point 30 of the extraction material, paragraph 60 analysis and paragraphs 40, 50 processing and sorting, respectively. Each PLC 70A, 70b, 70C are connected to the server 72 via a local area network (LAN). The server 72 is in communication with the database (which may be local, remote, or a combination) and write/retrieve data in the database and from the database in response to requests from individual PLC 70A, 70b, 70C. The server 72 is able to communicate with remote devices over a communication network such as the Internet 90.

In the illustrated embodiment, the LAN is a wireless LAN and PLC 70A, 70b, 70C communicate with the server 72 via a bridge circuit 71A, 71b, s workgroups wireless LAN. However, it should be clear that it is possible to use a LAN of any type, including LAN network with a wired connection. For example, on figa-13D illustrates an implementation option LAN with wired tie the Y.

In the illustrated embodiment, the PLC 70A is designed to control paragraph 30 of the extracted material to remove material from a variety of eggs, as described above. PLC 70A is also designed to control subsystem 74 detector for detection of live/dead (for example, the sorting machine 12, 11), the controller 75 of the device with the stepping movement of the two platforms, which manages the placement tray of samples for reception of material to be extracted from the eggs, the device 77 reading a bar code strips for eggs and device 78 reading a barcode tray of samples for analysis. In accordance with the variants of implementation of the present invention barcodes are used to track the eggs within an incubator workshop. For example, bar codes are placed on the strips for eggs and read over various periods of time during processing inside an incubator workshop. Other options for implementation include instead of barcode labels, RFID (radio frequency identification) and promptly printed/plotted IDs or gaskets for eggs, either directly on the eggs.

PLC 70b is designed to control paragraph 60 of conducting analysis to identify one or more characteristics of each of the eggs, as described above. PLC 70b also is designed to control subsystem 60 reader analyses (for example, system cameras on the CCD which scans each vessel for samples in the template for analysis to determine the sex of the relevant eggs, selected material which is in the vessel), the controller 81 of the device with the step of moving the reader analyses, the controller 82 of the device with the stepping movement of the distributor of the substrate, the controller 83 of the device with the stepping movement of the distributor yeast and device 84 reading a barcode analyses. In addition, the PLC 70b may be configured to paragraph 60 of the analysis, which is directly connected to paragraph 30 of the extraction material or is a stand-alone device.

PLC 70C designed to control point 40 processing and paragraph 50 of the sort, as described above. In addition, the PLC 70 C controls the device 85 reading a bar code strips for eggs, which identifies strip for eggs passing through the points 40, 50 processing and sorting.

Figa-13D represent a more detailed illustration of the architecture of the tools top-level management system for processing eggs in accordance with the variants of implementation of the present invention inside an incubator shop where individual PLCs are used to control point extraction material (module takes the image of the standard module analysis module and move accordingly. Illustrated on figa-13D variant implementation uses an implementation option LAN with a wired connection, in which the system server (figa) communicates with the module sampling (sigv), module testing (figs) and module move (pigv) (and manages).

Below the point of extraction of the material.

On Fig-17 shows 30 extraction of material designed to extract material from a variety of eggs, in accordance with the variants of implementation of the present invention. Paragraph 30 of the extraction material includes a frame 100 with the incoming conveyor system 102 gaskets for eggs and output conveyor system 104 gaskets for eggs, passing, respectively, along opposite sides 100A, 100b frame 10C, as illustrated in Fig. Paragraph 30 of the extraction material also includes sorting machine 12 (Fig), which was designed with the purpose of identifying live eggs among many eggs, the platform 110 nests for eggs, moving image mounted on the frame 100, the device 130 move eggs, system 150 manipulating trays for samples, a set of four devices 160 sampling system and disinfectants (not shown) for disinfecting parts of the sampling device.

the bound conveyor system 102 gaskets for eggs formed for transportation of incoming strips for 5 eggs 1 through the sorting machine 12, and the device 130 move the eggs. As will be described below, in accordance with the embodiment of the present invention the living eggs are removed from the incoming strips 5 for eggs. Inanimate eggs remain inside of incoming strips 5 for eggs and are carried away by the incoming conveyor system 102 gaskets for eggs to eliminate or other processing. Output conveyor system 104 gaskets for eggs, in accordance with the embodiment of the present invention, designed for transporting strips 7 for eggs from which it learned the material in the incubator for incubation and/or at subsequent points of treatment and/or sorting.

Embodiments of the present invention is not limited to the removal of live eggs from the incoming strip 5 for eggs. For example, from the incoming strip 5 for eggs, you can remove all the eggs and place in the matrix of nests for eggs. Live eggs can be separated from non-living eggs by paragraph 50 of the sort (11). For example, using 50 sorting in the container for removal can be transferred only live eggs.

In the incoming conveyor system 102 for eggs, you can use tape and/or other components of the conveyor system, which provide the possibility of light passing through a portion of it to facilitate Boscoreale and sorting machine 12. Specialists in this field of technology is known and conveyor system gaskets for eggs, and it is not necessary to describe here. In addition, embodiments of the present invention is not limited to the illustrated orientation, configuration and/or directions of movement of incoming and output conveyor systems 102, 104. The incoming and outgoing strip for eggs can be moved in different directions on different devices according to the invention, and they may have different configuration and orientation.

Although eggs are traditionally transferred into strips for eggs, you can use any way to move multiple eggs to the sorting machine 12 to identify live eggs. Eggs can pass through the sorting machine 12 by one or sorting machine 12 can be constructed so that through the sorting machine 12 could be held at the same time a lot of eggs (i.e. eggs inside the strip).

In accordance with the variants of implementation of the present invention in fact you can use the incoming and outgoing strip 5, 7 for eggs of any type. Strip can contain any number of rows, for example, seven rows of eggs, where six and seven rows are the most acceptable. In addition, eggs in adjacent rows may be parallel to each other, as in "rectangular" pad, or may be staggered, as in "offset" pad. Examples of appropriate commercial gaskets for eggs include, but are not limited to, the gasket "chick master 54"strip "JAMESWAY 42" strip "JAMESWAY 84" (in each case, the number specifies the number of eggs, which carries the strip). Strip for eggs known to experts in the art and will not be described here further.

In addition, the configuration matrix of the eggs from the incoming strips 5 for eggs may differ from the configuration of the output pads 7 for eggs. The device 130 move the eggs are designed to adapt to different configurations of the matrix of eggs from different gaskets for eggs, as described below.

Illustrated platform 110 nests for eggs includes first, second and third sets of slots 112 for eggs that are located adjacent the respective first, second and third matrices a, 113b and s. Illustrated platform 110 nests for eggs slidable mounted on the frame 100 between the incoming and output conveyor systems 102, 104, and can move relative to the device 130 move the eggs of each of the four illustrated device 160 sampling in the direction indicated by the arrows A1. Platform 110 nests for eggs is designed to move in such a way that, when one matrix nests (for example, a, or 113b or s) location is on under device 130 move eggs, another matrix nests (for example, a, or 113b or s) is located under one of the devices 160 sampling, as will be described in detail below.

Although the device for extracting material from eggs is shown with three matrices a, 113b, s nests for eggs and four devices 160 sampling, in accordance with the variants of implementation of the present invention it may have one or more matrices of the slots 112 and one or more devices 160 sampling. For example, a device for extracting material from eggs in accordance with the variants of implementation of the present invention may have one matrix of slots 112 and one device 160 sampling.

On Fig shows a detailed enlarged scale lifting cylinder 132 illustrated device 130 move and two of the devices 160 sampling Fig on opposite sides of the device 130 move. Illustrated lifting cylinder 132 includes an extensible and retractable matrix manifold blocks and suction cups 137, which are supported in a common rectangular frame 138. Potemkim head 132 designed to be lifted from the many eggs from a matrix of slots 112 and placing the eggs inside facing strips 7 for eggs.

Illustrated platform 110 nests for eggs includes many of elongated rods 118, which simultaneously control the controlled drive unit 122, which moves the elongated rods 118 between retracted and released positions (indicated by the arrow And2) within their respective slots 112, to change the provisions of the eggs from the horizontal to the vertical, as will be described below. Each device 160 sampling includes a matrix of heads 162 sampling, which are designed to extract the material from the eggs inside the nest 112 for eggs. Each head bribe samples designed essentially for vertical movement (indicated by arrows And3) relative to the platform 110 nests for eggs, as will be described below.

Fig is a side view in vertical section of the device of the extraction material Fig illustrating two lifting heads 132, 134 device 130 move the eggs. As shown, the lifting head 132, 134 are designed for lateral movement (indicated by arrows And4between the incoming and output conveyor systems 102, 104 gaskets for eggs and the platform 110 nests for eggs.

Figa-18D illustrate the progress of the eggs through the point 30 of the extracted material. Figa illustrates loading of incoming strips 5 for eggs, which contain many eggs 1 on the incoming conveyor system 102 gaskets for eggs and loading of empty pads 7 for eggs on the output envelopes the policy system 104 gaskets for eggs. Figa also illustrates the incoming strip 5 for eggs containing many eggs 1 located in the area of Boscoreale (i.e. under the sorting machine 12 shown in Fig) device 3D extraction of the material.

Figv illustrates the movement of the incoming strip 5 for eggs on the incoming conveyor system gaskets for eggs from Boscoreale in the region of the grasp. 3 the area of a gripping device that moves the head 134 of the eggs is designed to capture multiple eggs 1 from strips 5 for eggs and placing eggs 1 inside the matrix of slots 112 on the moving platform 110 nests for eggs. Blank facing strip 7 for eggs is located next to the matrix of slots 112.

Figs illustrates many eggs 1 placed within a set of 112 nests for eggs after their transfer from the incoming strips 5 for eggs. For simplicity of illustration eggs 1 shows inside the slots 112, essentially in a vertical orientation. However, as will be described below, the position of the eggs 1 changes the slots 112 for eggs, essentially, to a horizontal orientation before removing material from the egg 1. Socket 112 for eggs are also designed to change the position of the eggs after the material of which the extracted essentially vertical orientation, to move the eggs in the facing strip 7 for eggs.

Eggs 1', which drew m is a material predetermined, transferred to the facing strip for eggs. Facing strip 7, which are eggs that have just took the samples, after that can be placed in the incubator for incubation in accordance with the usual procedures while waiting for the results from paragraph 60 analysis (11). When the analysis results obtained and the characteristics of each of the eggs identified (e.g., gender), eggs can be moved from the incubator to one or more points 40 of the handle (11) and/or paragraph 53 sorting 11). In accordance with the variants of implementation of the present invention, described below, paragraph 60 of the analysis can be connected with paragraph 30 of the extraction material and can be configured for fast analysisone material extracted from eggs. Spacers for eggs from which the extracted material can be held in one or more modules accumulation instead of returning to incubator to transport the item (items) processing/sorting.

Fig.18D illustrates the moving platform 110 nests for eggs in the direction indicated by the arrow A1to the location where the matrix 112 nests for eggs, containing eggs 1 located under one of the devices 160 sampling (Fig).

Fig illustrates a portion of an exemplary matrix of slots 112, which can be placed on illustrisimo the platform 110 nests for eggs. Each slot 112 is designed to receive eggs, essentially in a vertical orientation and movement of eggs, essentially in the horizontal and centered orientation.

An enlarged perspective view of the socket 112 in the illustrated partial matrix Fig shown in Fig and is indicative for each slot in the partial matrix. Illustrated slot 112 includes an inclined, arcuate surface 114 that defines a reservoir for receiving eggs. Illustrated arcuate surface 114 of the slot 112 has an inclined upper portion of a, the lower portion (or bottom) 114b and opposite side areas of a, 115b.

The arcuate surface 114 nests can be in General a concave configuration between the opposite lateral areas a, 115b. In General concave configuration of the arcuate surface 114 helps to keep the egg, essentially, and centered position on the arcuate surface 114. The upper portion of a the arcuate surface is designed to receive the end of a vertically oriented egg and ensure shedding eggs to the lower section 114b arcuate surface so that the egg is mounted on the lower section 114b arcuate surface, essentially in an inclined orientation.

Embodiments of the present invention is not limited to the illustrated socket 112 and the and illustrated by the configuration of the arcuate surface 114. The arcuate surface 114 of the slot 112 may be essentially smooth, continuous arcuate surface. Alternatively, the arcuate surface 114 may include many flat adjacent surfaces located so as to form a generally arcuate configuration. In addition, the arcuate surface of the socket may be essentially flat configuration between the opposite lateral areas a, 115b.

Nests for eggs, which are designed to receive eggs, essentially in a vertical orientation, movements eggs, essentially in a horizontal orientation and reorientation of eggs, essentially in a vertical orientation to retrieve described in application for U.S. patent No. 09/835990.

Each illustrated socket 112 also includes a pair of elongated retaining levers 119, attached to the socket 112 at a distance from each other along respective side portions a, 115b arcuate surface, as illustrated. Each of the illustrated elongated lever 119 has a corresponding end a, which is attached to the socket 112 using fasteners 120, and an opposite free end 119b. Fasteners 120 may be of various known fixing devices, including, but not limited to, threaded fasteners (such as screws, bolts etc.) and nerushimoye fasteners (e.g. rivets, tapering pins, not narrowed pins etc). Alternatively, the retaining levers 119 can be attached to the socket 112 glue or attached to the socket 112 for eggs by welding, brazing, soldering, soft soldering, or various other known methods.

The retaining levers 119 help prevent it from rolling or falling eggs with the arcuate surface 114 of the socket. In addition, the retaining levers 119 to help stabilize the egg, which changes the position, essentially, from a horizontal position to an essentially vertical position, as described below. Holding the lever 119 is designed to bend outward, as shown in Fig to accommodate large eggs, while at the same time provide support for a narrow eggs. In addition to the toga, holding the levers 119 help to put the egg in the centre sideways on the arcuate surface 114 of the socket so that the long axis of the egg was not aligned with the long axis of the slot, while the egg is essentially in a horizontal position.

Embodiments of the present invention is not limited to the illustrated retaining levers 119. The retaining levers can have various configurations and can be attached to the socket 112 in different location is the third and configurations. Moreover, for some embodiments of the present invention holding the levers may not be required.

Each slot 112 is attached to the platform 110 nests through the fixing device, including, but not limited to, threaded fasteners (e.g. screws, bolts, etc. and nerushimoye fasteners (e.g. rivets, tapering pins, not narrowed pins etc). Alternatively, each slot 112 can be attached to the platform 110 nests glue or attached to the platform 110 nests by welding, brazing, soldering, soft soldering, or various other known methods. Fig illustrates threaded through holes 121 in the slot 112, which are made for the threaded coupling respective threaded fasteners (not shown) for the mounting slot 112 of the platform 110 nests in accordance with the variants of implementation of the present invention.

Many through-holes 116 pass through each slot 112 and terminate at the respective bores 117 in the arcuate surface 114, as illustrated. An elongated rod 118, which serve as an element for orientation, is designed for reciprocating movement between a retracted position and a released position within each bore otverstia. In the released position, the elongated rods 118 for each slot 112 push horizontal egg (or, otherwise, is inclined relative to the vertical) on the lower section 114b arcuate surface to a vertical orientation so that the egg can be removed from the slot 112 through the device 130 move the eggs.

Embodiments of the present invention is not limited to the illustrated elongated rods 118 or orientation of elongated rods 118 relative to each slot 112. Elements for orientation can have various configurations and can be installed inside the socket 112 for reciprocating movement between retracted and released positions in a variety of ways and in different orientations.

As shown in Fig, elongated rods 118 are located in the matrix and simultaneously controlled by a drive mechanism 122, which moves the elongated rods 118 between retracted and released positions within their respective slots 112. When the matrix of rods 118 is retracted, the eggs inside the slots 112 are essentially horizontal orientation, as described above. When the rod 118 is moved to a released position, the rods pass up through the slot, as described above, and cause the eggs to move generally in a vertical orientation. The actuator 122 is La movement of rod 118 between retracted and released positions can be controlled pneumatically, hydraulically, magnetically, and/or you can use Electromechanical actuators.

Fig-26 illustrate the socket 212 for eggs, which can be used in accordance with other variants of implementation of the present invention and which is designed to change the position of the eggs from a vertically oriented position to a horizontal position and then back to a vertically oriented position according to the alternative implementation of the present invention. Shows the slot 212 has first and second sections a, 220b, which determine the capacity for the reception of the eggs. Illustrated first section a has a pair of opposite, spaced at a distance elements 222, 224 with inclined upper ends a, a. Each inclined upper end a, a has the sloped surface 226, 228. Illustrated second section 220b has a pair of opposite, spaced at a distance elements 232, 234 with inclined upper ends a, 234a. Each inclined upper end a, 234a has a beveled inside surface 236, 238.

Inclined upper ends a, 234a of the second section 220b is made to receive the end of a vertically oriented egg and ensure slip eggs down so that the egg is located on the first and second sections a, 220b, essentially, in an inclined orientation. Configuration Naklo the data of the upper ends a, A, a, 234a of the first and second sections a, 220C helps to keep the egg in the nest 212, essentially, in a Central position.

The second section 220b serves as an element for orientation and constructed for reciprocating movement between a retracted position (Fig) and a released position (Fig). In the released position, the second section 220b leads horizontal egg (or, otherwise, is inclined relative to the vertical) inside the socket 212 to a vertical orientation.

As shown in Fig, the device 130 move the eggs of paragraph 30 of the extraction of the material depicted on Fig includes first and second adjacent lifting heads 132, 134, which work in tandem. The first lifting cylinder 134 is designed for simultaneous rising of many, essentially vertically oriented egg 1 from the incoming strip 5 for eggs on the incoming conveyor system 102 gaskets for eggs and placing many eggs 1 inside the first matrix of slots 112. Eggs are usually installed inside of the incoming strip for eggs with the blunt end of the egg facing essentially upward. The first lifting cylinder 134 can be controlled to capture selected eggs 1 from the incoming strip 5 for eggs. For example, the first lifting cylinder 134 may be utilized to capture live eggs identified by the sorting machine 12.

Adjacent the second lifting cylinder 132 is designed for simultaneous lifting and removal of many eggs 1 from a variety of slots 112 of the platform 110 nests for eggs and placing eggs 1 inside facing strip 7 for eggs on the output conveyor system 104 gaskets for eggs. Eggs 1 reorienting essentially in a vertical orientation to facilitate their removal from the slots 112. Eggs are usually placed inside a facing strip for eggs with a blunt end, aimed, essentially, upwards.

Illustrated platform 110 nests for eggs moving image is mounted on the frame 100 and is movable relative to the first and second lifting heads 134, 132 so that the first, second, or third matrix a, 113b, s slots 112 for eggs can be installed under device 130 move the eggs at any given time, to lifting heads 132, 134 could place the eggs inside the slots 112 or removing them therefrom, as described above.

Moving the configuration of the platform 110 nests for eggs enables one matrix nests to take eggs from one of the winding heads 132, 134, while the other matrix nests installed under the appropriate device 160 sampling so that the eggs can be retrieved material, as will be described below. The use of multiple matrices nests for eggs along with back-Postup the positive movement of the platform nests for eggs increases processing performance.

On Fig-29 each lifting cylinder 132, 134 illustrated device 130 move eggs includes an extensible and retractable matrix manifold blocks 136 and the suction cups 137, which are supported essentially rectangular frame 138. Illustrated frame 138 includes opposite side elements 139a, 139b, passing along the first direction L1and opposite end elements 140A, 140b, which are along the second direction of the L2which is essentially perpendicular to the direction L1.

Each manifold block 136 and vacuum suction Cup 137 is supported using the appropriate cross beam 142 which, as shown, passes between the side elements 140A, 140b. The middle one of the transverse beams is fixed between the side panels 140A, 140b. Cross beam 142 on either side of the recorded medium cross beam moving image supported by the frame 138 and configured to move along the second direction of the L2. The adjacent cross beam 142 is connected through a pair of restraining elements 143.

The drive elements 144A, 144b are connected with beams 142, as shown, and are used for folding and extending of the matrix manifold blocks 136 and the suction cups 137 along the second direction of the L2. Each of when adnych elements 144A, 144b is controlled by a drive mechanism 145, which is in communication with the control device (e.g. PLC 70A on Fig). The actuator 145 can be controlled pneumatically, hydraulically, magnetically, and/or you can use Electromechanical actuators.

Fig illustrates a matrix manifold blocks 136 and the suction cups 137 in the extended configuration, and Fig illustrates a matrix manifold blocks 136 and the suction cups 137 in a folded configuration. On Fig restrictive elements 143 are not shown for clarity. Expandable and collapsible nature of the matrix manifold blocks 136 and the suction cups 137 for each lifting head 132, 134 provides an opportunity to raise many (or "grip") eggs from gaskets and matrices nests for eggs of different sizes and configurations to insert in them.

In accordance with the variants of implementation of the present invention, the matrix manifold blocks 136 and the suction cups 137 can grow and develop in two directions. For example, the specific structure of the incoming strip for eggs can provide one inch (1") between adjacent eggs in a row and one inch (1") between adjacent rows. Matrix 112 nests for eggs in the platform 110 nests for eggs may have a different configuration. For example, the matrix nests for eggs can only provide half inch (0,5") between the adjacent eggs in a row and a half inches (1.5 inch) between adjacent rows. Similarly facing gasket eggs can be configured as a matrix, which differs from the configuration matrix nests for eggs. Matrix, which is able to expand and develop in two directions, can adapt to these differences in the matrices pads and nests for eggs.

The configuration matrix of each lifting head 132, 134 can be adjusted by means of the control device, such as a Central controller (PLC) or a separate controller (PLC) (e.g., PLC 70A on Fig) so that eggs could migrate between the strips for eggs and nests of different sizes and/or configurations of matrices. Each lifting cylinder 132, 134 also preferably easily removed, as the host, to facilitate cleaning.

On Fig each manifold block 136 includes a face area a and internal penetration hole 144 which terminates in nozzle 149 extending from the end section a. Internal penetration hole 144 of each manifold block 136 is in fluid communication environment with a vacuum source (not shown) and the air source through the respective vacuum and air lines connected to the corresponding sockets on the top of each manifold block 136, as should be clear to experts in the given field of technology. Each manifold block 136 and Waco is MNA sucker 137 are preferably in fluid communication environment with a separate source of vacuum, to enable selective movement of the eggs.

Flexible vacuum suction Cup 137 is attached to each respective nozzle 149 manifold block. Each flexible vacuum suction Cup 137 is designed to capture and hold the eggs are fixed thereto by the way, when within the flexible suction Cup 137 provide a vacuum through the appropriate internal penetration hole 144, and the corresponding release of the egg, when the vacuum inside the corresponding inner bore holes 144 cancelled. You can serve the air from the air source into the internal bore hole 144 to facilitate the removal of eggs from a flexible suction 137.

For lifting heads 132, 134 device 130 move the eggs you can use different lifting suction device type. In addition, in accordance with the variants of implementation of the present invention can use any suitable method for transferring the eggs from laying in a matrix of nests for eggs and die nests for egg laying.

Each device 160 sampling device 30 extraction of material on Fig includes a matrix or set of 161 heads 162 sampling. Each cylinder 162 sampling designed to extract material from the egg and put the extracted material inside the corresponding vessel of the 152 samples on the tray 150 for samples (Fig). Each device 160 sampling in illustrated by Fig embodiment, is fixed, and the platform 110 nests moves relative to it, as described above. Accordingly, when the set of 112 nests containing eggs 1 located under the device 160 sampling, each cylinder 162 sampling performed with the possibility of removal of material from the respective eggs 1 and the subsequent distribution of the extracted material in an appropriate vessel 152 to the sample tray 150 for samples.

On Fig each cylinder 162 sampling according to the illustrated variant implementation includes an elongated body 163 having opposite first and second ends a, 163b, and an elongated bore hole (guide device) 164, which takes place between them. Elongated needle 165 is placed inside the elongated passing hole 164 and are able to move between the retracted position and the first and second released positions. The sharp end 166 of the needle 165 is contained within bore holes 164, when the needle 165 is retracted, and the sharp end 166 of the needle 165 protrudes from the first end a housing when the needle 165 is in the first and second released positions. The needle 165, when it is in the first extended position, is adapted to pierce holes through skorl the PU eggs and extract material (for example, allantoine liquid) from the egg. The needle 165, when it is in the second extended position, is adapted to release the extracted material eggs in an appropriate vessel for the sample tray of samples, as described below.

Needle 165 may be a syringe needle having a pointed configuration of the end connectors shell. In accordance with the variants of implementation of the present invention the pointed end 166 of the needle may have a beveled or blunted configuration to facilitate puncturing holes through the shell of the egg. In accordance with options for the implementation of the present invention, the needle 165 may have a hole formed in the side part thereof, instead of a pointed end 166 to help prevent clogging of the lumen of the needle caused by puncturing holes through the shell of the egg. Needle 165 heads for sampling in accordance with the variants of implementation of the present invention, in particular, adapted to extract allantoin fluid from the eggs.

As is well known to specialists in this field of technology, allantoine liquid represents excretory nutrient medium for nitrogen metabolites avian embryo. Allatoona liquid begins to form about a 5 day incubation. It reaches a maximum size of approximately 13 days of incubation and C is the decrease in volume with continued incubation, due to the moisture loss and resorption of fluid, but is still present in significant quantities on the 13th day of incubation.

Allatoona liquid separated from the egg shell internal and external skorlupoy shells and chorioallantoic membranes. Although allatoona fluid surrounds the entire periphery of the eggs with the developing embryo, allantoine liquid accumulates at the top of the eggs just under the membrane covering the air cavity. Accumulation allantoine liquid at the top of the eggs due to gravity and offset dense embryo and the yolk SAC. Attempt to accurately sample allantoine fluid through the top of the egg while the egg is located vertically, can be difficult due to the variability of the airspace from egg to egg. You can use gravity to combine allantoine fluid in a localized location. When the egg is rotated along its longitudinal axis, allantoine liquid is merged into the top side of the egg, right under the shell. The location of the egg along its longitudinal axis leads to easier access to allantoine liquid.

Removing material type allantoine liquid eggs can be performed in various ways in accordance with the variants of implementation of the present invention. For example, if only the live eggs are initially placed inside the slots 112 of the platform 110 nests for eggs, sampling will be conducted of all eggs. However, if inside the slots 112 of the platform 110 nests for eggs placed inanimate eggs, sampling will be made only from fresh eggs. Alternatively, in the shell of all eggs, including inanimate eggs, can pierce holes, but the material will be taken only from living eggs. In accordance with alternative options for implementation of each cylinder 162 sample may contain a biosensor or other device for analyzing material eggs (for example, allantoin liquid) in situ (in place). As will be described below, according to other variants of implementation of the present invention the removing material of the egg and the analysis of the extracted material can be accomplished in one and the same sampling device.

Each cylinder 162 sampling illustrated in Fig variant implementation also includes an element 166 alignment. Illustrated element 168 alignment includes a section 169 of the housing, which is movable attached to the first end a head housing and sampling. Two pairs of opposite wheels a, 170b are installed on opposite end sections a, 171b section 169 of the housing.

As illustrated in Fig, 1 egg is held in place inside the socket element 112 168 alignment is when the head 162 of sampling is provided in contact with the egg inside the socket 112. Element 168 alignment adjusts the position of the egg and places it in the center inside the socket 112. In the illustrated embodiment, opposite the wheels a, 170b are in contact with the shell of the egg along with the first end a head housing sample.

Embodiments of the present invention is not limited to illustrated on Fig configuration of the head sample. For example, the head of the sampling may be an element of levelling without a pair of opposite wheels a, 170b. In addition, embodiments of the present invention can use the alignment elements having different shapes, sizes and configurations.

The actions of the head of the sampling is shown in Fig-35. Fig is a side view of the many heads 162 sampling for one of the four illustrated by Fig devices 160 sampling. Each head sample is in contact with the egg shell 1 within the slot 112 for eggs before removing material from the egg 1, and the needle 165 sampling within each head sampling is retracted. In addition, the illustrated actuator 180, moving the lever 182 through the drive piston 181 from the first position to the second position, as indicated by the arrow a5. The lever 182 is connected with a blocking place the Incas 185 heads, sampling, which a moving image is placed between the fixed plates 186 and 187. As will be described below, the locking plates 185 are designed to maintain each head 162 sampling in the vertical locked position relative to the corresponding eggs 1 inside the socket 112, when eggs 1 remove the material.

On Fig lever 182 is moved into the second position so that the blocking plate 185 separated by distance in the locked position, to limit the vertical movement of the heads 162 sampling. Needle 165 sampling extended to the first released position and pierced the shell of each respective eggs. In the first position of the needle 165 sampling are in position to extract material (for example, allantoin liquid) of each respective entity.

On Fig lever 182 is moved back to the first position so that the blocking plate 185 not restrict the vertical movement of the heads 162 sampling. Needle 165 sampling extended to the second released position and are in a position to distribute the material extracted from the relevant eggs into the corresponding receptacles 152 for samples in the matrix 150 for samples. The second released position provides sufficient clearance for the head 162 of the sampling and/or element 168 exp is vnimanie for to needle 165 could reach 152 vessels for samples in the tray 150 for samples and to needle 165 could reach a cleansing nozzle or other device that produces a disinfectant the fluid in the needle 165.

Embodiments of the present invention is not limited to heads of sampling, in which needles are first and second released position. According to a possible alternative implementation of the needle can be moved from venutolo position only one released position to extract material from eggs. For distribution of the extracted material in the vessel for sample tray for samples, you can move up to the needle. Similarly, you can move up to the needle cleansing nozzle or other device.

The movement of the needle 165 sampling inside the head 162 of sampling is illustrated in more detail figa-36C. Each cylinder 162 sampling includes a bias element (e.g., spring) 190, as shown in figa. Moving each needle 165 sampling of venutolo provisions in the first and second released position due to air pressure (or the pressure of the other fluid), which is provided from a compressed air source (or source of another fluid). To move the needle 165 sampling of venutolo position in the first floor released the Appendix (pigv) is the pressure of air (or other fluid) at the level sufficient to overcome the force of the air displacement in the lower half of the head 162 of sampling (for example, 28 psi (pounds per square inch)), but not sufficient to overcome the combined force air displacement in the lower half of the head sampling and bias element 190. To move the needle 165 sampling of venutolo position to a second released polecenia (figs) is the pressure of air (or other fluid) through one or more nozzles (not shown) in the cylinder 162 sampling at a level sufficient to overcome the combined force air displacement in the lower half of the head sampling and bias element 190 (e.g., 75 psi).

In the illustrated embodiment, the bias element 190 is designed to push the needle 165 sample released from the second position to the first released position when the air pressure inside the lower half of the head 162 of sampling is reduced. The air pressure in the lower half of the head 162 of sampling is increased to move the needle 165 sampling in stenothoe position. Bias element 190 may have various shapes, configurations and/or sizes and is not limited to the specific embodiment.

In the illustrated embodiment, air is supplied through the nozzle 192 in each cylinder 62 sampling for drying the outer sections each corresponding needle 165 sampling after the disinfection of each respective needle 165 sampling.

On fig.36D shows an example of disinfectant fountain 200 that can be used for sterilization of the corresponding needle 165 sampling in accordance with the variants of implementation of the present invention. Illustrated fountain 200 has formed in it the hole 201, which is intended to receive the corresponding needle 165 sampling. Disinfecting fluid is supplied to the fountain from the source through the supply pipe 202. The fountain 200 includes one or more nozzles (not shown), which are designed for spraying needle 165 sample of the disinfectant fluid medium. In accordance with the variants of implementation of the present invention provided with a matrix of fountains 200 so that the needle 165 sampling from the corresponding matrix heads 162 sampling can be omitted in appropriate fountains 200 Ambassador put the extracted material eggs in the vessels of the sample tray of samples. However, embodiments of the present invention is not limited to the illustrated disinfectant fountain 200. For filing a disinfection fluid in needle sampling can be applied disinfection systems using different types of devices.

On Fig 38A-38B and 39A-39C referred to in the subsequent description of the blocking plates 85. Fig is a top view of a matrix heads 162 sampling, taken along the lines 37-37 on Fig, which illustrates the locking plates 185. Illustrated locking plates 185 include many holes 300 formed there in a matrix structure matrix heads 162 sampling. Each cylinder 162 sampling is performed so that the slidable positioned within corresponding holes 300, and is designed to move freely in the vertical direction, when the locking plates 185 are not locked in the statement.

Within each illustrated openings has a pair of elastic arms 302, which is designed for the application bias force to the corresponding cylinder 162 sampling, when the blocking plate 185 is moved to the locked position. Elastic arms 302 are designed to prevent clogging of the device because of one head sampling, which is somewhat larger than the other heads of sampling, and prevent other heads of sampling from locking in place. In the illustrated on figa embodiment, the locking plates 185 are removed from each other when mixed in a locked position. However, embodiments of the present invention is not limited to Il is yustiruemye locking plates 135 or their direction of movement.

File illustrates the locking plates 185' according to other variants of implementation of the present invention. Illustrated locking plates 185' include many holes 300 formed there in a matrix structure matrix heads 162 sampling. Each cylinder 162 sampling is performed so that the slidable positioned within corresponding holes 300, and is designed to move freely in the vertical direction, when the locking plates 185' are not in the locked position. In the illustrated on FIGU embodiment, the locking plates 185' are also removed from each other when moving in the locked position.

Within each illustrated openings has a pair of elastic arms 302', the control unit 303 and the spring 304, connected to the elastic levers 302', which are designed for applying bias force to the control unit 303. When the locking plates 185' move relative to the fixed upper and lower plates, elastic levers 302' hook corresponding cylinder, sampling, and springs 304 impose a bias force to the block 303, which limits the vertical movement of the head sample. As in the embodiment according figa, elastic levers 302' designed for PR is to prevent clogging of the device because of one head sampling which is somewhat larger than the other heads of sampling, and prevent other heads of sampling from locking in place.

Embodiments of the present invention is not limited to the illustrated locking plates 185 on figa-38B. You can also use a blocking plate having a different configuration. In addition, you can use other ways to limit the movement of the heads of sampling (for example, see U.S. patent No. 5136979).

The movement of the locking plates 185 shown in figa-39C. On figa, as illustrated, the blocking plate 185 is in the unlocked position, and the cylinder 162 sampling is free for vertical movement within the holes 300 a locking plates 185 and corresponding holes a, a in the upper and lower fixed plates 186, 187. On FIGU blocking plate is moved to the locked position (indicated by arrows A6), so that the blocking plate 185 pushes the head 162 of the sample in the direction of the upper and lower stationary plates 186, 187. On pigs head 162 sampling jams relatively stationary upper and lower plates 186, 187 blocking plate 185 so that the vertical movement of the head 162 sampling is limited.

Turning now to Fig, mark is m, it illustrates an exemplary tray 151 for samples containing many blood vessels 152 for samples formed there in various matrices. Each vessel 152 for samples intended for receiving the sample material extracted from the eggs, such as allatoona liquid. In accordance with the variants of implementation of the present invention can be used trays for samples with different configurations and matrix vessels for samples. Sample trays can be manufactured from different materials and using different technologies. The present invention is not limited to the illustrated tray 150 for samples.

Fig represents an enlarged partial top view of the tray of samples on Fig illustrating the material extracted from eggs placed within the respective receptacles of the sample tray of samples. Material extracted from eggs, you can distribute within their respective receptacles 152 for samples of the tray 150 for samples under various schemes of the distribution. For example, as illustrated in Fig, the material of the eggs with a particular strip can be placed inside the first vessel 152a in the first row of the group of vessels. The material of the eggs in the next strip can be placed in the second vessel 152b in the first row, etc. distribution Circuits preferably run with the help of the panel is engaged in the device (for example, PLC 70A on Fig).

Figa-42V represent the top of the system 150 manipulating trays for samples in accordance with the variants of implementation of the present invention and illustrating the trays 151 for samples transported (as indicated by the arrows And7) relative to the device 160 sampling Fig (i.e. underneath). Because each device 160 sampling illustrated device extraction material Fig still, the system 150 manipulating trays for samples intended for the movement of vessels 152 for samples under appropriate heads 162 sampling so that the material extracted from the eggs may be placed within the respective vessels for samples. After the vessel 152 to the sample tray 151 samples have been extracted from the egg material, the trays 151 to unload samples (either manually or automatically) and extracted from the egg material allow to dry. As soon as the tray 151 for the sample is unloaded, the system 150 manipulating trays for samples moved back to receive a new tray 151 for samples loaded by the operator.

Although not shown, the system disinfectants preferably provided with the illustrated device 30 extraction of material on Fig. For example, disinfectants may be Opera is actively connected to the heads 162 sampling each device 160 sampling and is intended for injection of disinfectant fluid through the head 162 sampling and around their outer sides, including extra long needle 165 and through holes 164 for the needles. For example, illustrated in fig.36D the fountain 200, which is designed to summarize the disinfectant fluid to the needle 165 sampling. Disinfecting fluid preferably is served on every part of the head 162 sampling, which comes in contact with the egg, after distribution of material extracted from the eggs within the vessel 152 for samples in the tray 150 sampling. Means for drying each of the head 162 of the sampling needle 165 and bore holes 164 are provided, preferably, after the filing them in disinfectant fluid. For example, it is possible to provide a system for directing air into each cylinder 162 sampling needle 165 and the penetration hole 164. In the illustrated on figa-36C embodiment, the drying air supplied through the nozzle 192.

Examples of systems disinfecting fluid, which are designed to provide a disinfection fluid and which can be used in accordance with the variants of implementation of the present invention described in U.S. patent No. 5176101 and reissued U.S. patent 35973.

Embodiments of the present invention is not limited to the illustrated device 30 extraction of material on Fig or exact process described above. Each of the components of the clients (device 130 move eggs, platform 110 nests for eggs, the device 160 sampling, conveyor systems 102, 104 gaskets for eggs) can work in various ways, until the material extracted from the eggs can be identified as coming from that particular egg.

With reference to Fig-46 will be described below paragraph 60 analysis and how to use paragraph 60 analysis to determine characteristics of eggs in accordance with the variants of implementation of the present invention. Illustrated paragraph 60 of the analysis is intended for processing multiple trays for samples containing material extracted from eggs, as described above, to determine one or more characteristics of the eggs.

On Fig-44 region 410 storage is designed to receive and store multiple trays for samples containing material extracted from a variety of eggs within a predetermined period of time. Then, each tray of samples is transferred from the area 410 of the storage area 420 of the application of the biosensor (e.g., yeast), where the biosensor is added to the vessels for samples in each tray of samples. After that, each tray of samples takes place in the region 430 of the application of coloring, where a coloring substrate (for example, OPNG-substrate) is added to the vessels for samples in each tray of samples. In General, the BIOS is rubbish and a coloring substrate is added to the dried material (for example, allantoine liquid), extracted from egg to cause a chemical reaction that can change the color of dried material on the basis of the characteristics (e.g., gender) eggs. After passage of a predetermined period of time each tray of samples moved to the area 450 "read", and the color of the material in each vessel for samples analyzed to determine characteristics. For example, if you want to define characteristic is gender, the material extracted from the eggs of the females may have a color that is easily distinguished from the color of the eggs the male. Before you can remove the trays for samples, it is preferable to destroy biosensor using region 460 decontamination.

In accordance with the variants of implementation of the present invention, paragraph 60 of analysis is particularly suited for determining the sex of the eggs. The operator loads a set of templates for samples containing material (for example, allantoin liquid), extracted from eggs, in paragraph 60 of the analysis. Inside the module 60 analysis of each pattern for the sample is moved by a conveyor system under distribution head which dispenses a predetermined number (for example, approximately 75 μl of the reagent (for example, marking biosensor cellular basis LiveSensors™, LifeSensors, Inc., Malvern, PA) in ka is every appropriate vessel for samples. Then each pattern for the sample is moved through the chamber with a controlled environment within a predetermined period of time (for example, approximately 3.5 hours). Each template for the sample is moved by a conveyor system under anotherdistribution head which dispenses a predetermined quantity of coloring substrate (e.g. substrate on the basis of ONPG) in each vessel for samples. Then each pattern for the sample is moved through the chamber with a controlled environment within a predetermined period of time (for example, approximately 45 minutes)to enable the development of staining within each cell.

Marking biosensor cellular basis LiveSensors™ used for determination of estrogenic compounds in allantoine fluid. An exemplary biosensor for the detection of cell-based LiveSensors™ is a genetically modified yeast transformed with expressionism a yeast vector for the human estrogen receptor, gene apomictical which contains the stimulant with elements of the reactions of estrogen associated with β-galactosidase of E. coli. B the presence of estrogen receptor estrogen is associated with elements of the reactions of estrogen and initiates transcription of the gene-obvestila. Concentrate the radio estrogen in allantoine fluid correlated with the level of induction of gene obvestila. The activity of the gene product of obvestila, β-galactosidase, measured using the substrate on the basis of ONPG, which gives yellow colorimetric signal. Marking biosensor cellular basis LiveSensors™ can determine femtomolar levels of estrogen. The strain of yeast marker biosensor cellular basis LiveSensors™ contains the same strain that is commonly used in the baking industry, Saccharomyces cerevisiae. Marking biosensor cellular basis LiveSensors™ can distinguish between embryos of males and females when using only about four microlitres (4 ál) allantoine liquid.

More precisely, the extracted allatoona liquid contains conjugates of estradiol, which is broken down by an enzyme (glucuronidase), secreted by the yeast during the initial cultivation allantoine fluid/sensor yeast. The presence of "free" estradiol easy system induces gene obvestila in yeast, producing Beta-galactosidase. Then Beta-galactosidase interacts with the substrate on the basis of ONPG added after cultivation allantoine fluid/sensor yeast, with the purpose of the formation of the dye signal.

In accordance with alternative ways of implementing the present invention, the yeast can be induced to selected what I GFP instead of Beta-galactosidase, which itself is fluorescent and does not require adding a colorimetric substrate.

The color of the material in each vessel for the samples can be determined in several ways. One method may include the lighting of the extracted material with white light and the use of cameras on the CCD (charge-coupled devices), which scans each vessel for samples and electronically filters out all colored signals except the signal with a specific color (for example, yellow, pink etc), that identifies the floor (e.g., females). Each tray of samples is preferably transparent, and the extracted material inside each tray for the sample is illuminated from below. The camera on the CCD can be configured to count the number of pixels (the minimum elements of the image color in the appropriate vessel for the samples to determine whether the number of pixels specified threshold. If so, then on the CCD camera can provide a digital signal signifying the presence of females in this location. This information is stored by the processor for processing data in the network.

Fig depicts the analysis using the detection biosensor cellular basis LiveSensors™ for various amounts of allantoin fluid (i.e. 4, 10, 20 µl).under each vessel for the samples indicated the intensity of the color (for example, yellow), measured on the CCD camera, in pixels. As shown, the females have a more intense yellow color than the males.

In accordance with the variants of implementation of the present invention, then the reagent (for example, marking biosensor cellular basis LiveSensors™) inside each cell is destroyed (for example by heating and/or chemical treatment) in region 460 decontamination prior to removal of each pattern for samples.

In accordance with the variants of implementation of the present invention, use of marker biosensor cellular basis LiveSensors™extracted from eggs specimen material, type allantoine fluid may contain more than approximately twenty percent (20%) impurities in the blood. In addition, the temperature of cultivation can range up to about five degrees Celsius (±5° (C), and the cultivation of samples can vary up to thirty minutes or more. In addition, samples, extracted from eggs, can be maintained within certain periods of time (e.g. over night) before the initiation of the processes analyzed in accordance with the variants of implementation of the present invention.

Another method may include the lighting of the extracted material with white light and the use of a matrix of photodiodes with filters. Each photodiode will be issued to the te signal, based on the color intensity, which he perceives.

Embodiments of the present invention is not limited to the techniques for analyzing yeast-based. In addition, embodiments of the present invention is not limited to identification of floor eggs. For analysis of material extracted from the eggs, you can use different techniques of analysis with the aim of identifying the different characteristics of eggs (e.g., gender, faecal pathogens, genetic markers associated with health or productivity of birds). For example, for the detection of estrogen in the material of the eggs, you can use the systems and methods based on antibodies (for example, commercial systems and methods of test for pregnancy). In addition, systems based on antibodies can be used to identify pathogens (such as Salmonella and Marek's disease). As another example, we can use the analysis of the ROC (the reaction of polymer chains) to determine the presence/absence of the W chromosome in the material of the eggs. In addition, ROC analysis can be used to identify different genetic features/defects in material eggs. Accordingly, the modules of the analysis can be such as to facilitate the identification of pathogenic organisms and genetic the WMD analysis of avian eggs.

On Fig illustrated device 60 point analysis in accordance with the variants of implementation of the present invention, designed for analysis of material extracted from the eggs contained within vessels for samples in many trays 151 for samples. As illustrated, the device 60 includes a number of chambers or areas, which are connected via a conveyor system designed for transporting trays for samples sequentially through the area. The field is preferably maintained at predetermined temperature levels and humidity. You can also use additional control of external conditions. For example, air can be pumped out of the device 60 through the fan 416 with a certain flow rate and can be filtered using a filtration system HEPA ("delay particles high performance").

As shown in Fig, many trays 151 for samples loaded with trucks 405 in the region 410 storage. Region 410 of the storage includes a first closed loop conveyor system 411, which is adapted for transporting multiple trays for samples at a distance from each other up to the 420 application of biosensor within a predetermined period of time. In the upper part of the storage area, each of the top tray is La samples on the first closed loop conveyor system arrives in region 420 of the application of the biosensor and the valves (not shown), intended for distribution biosensor (e.g., yeast) into corresponding receptacles of the sample tray of samples.

After the biosensor is distributed in vessels for samples tray of samples, the tray for the sample is transported through the second closed loop conveyor system 412 down towards the field 430 of the application of the coloring substrate. In the lower part of the second closed loop conveyor system 412 the bottom of each tray of samples arrives in region 430 of the application of the coloring of the substrate and under the valves (not shown), which is designed to distribute coloring substrate (e.g. substrate on the basis of ONPG) into corresponding receptacles of the sample tray of samples.

After coloring the substrate is distributed in vessels for samples of tray designs, tray for samples transported through a third closed-loop conveyor systems 413 down towards the field of 450 reading. In the lower part of the third closed-loop conveyor systems 413 the bottom of each tray of samples arrives in the region of 450 reading and one or more cameras on the CCD 415, which are designed to "read" the color of the extracted material in each vessel for samples as described above. Then biosensor in each vessel for samples destroyed by distribution the Oia in chemical product through the distribution head 417.

Paragraph 40 of the processing shown in 11 variants of implementation may be designed to selectively processing eggs any desired suitable way. In particular, it is assumed that paragraph 40 of the processing is injected into live eggs processing substance. As used here, the term "processing agent" refers to a substance that is injected into an egg to achieve the desired result. Chuck substances include, but are not limited to, vaccines, antibiotics, vitamins, viruses and immunomodulating substances. Vaccines intended for use in ovo in the fight against avian diseases when breeding birds are commercially available. Typically, the processing substance dispersed in a fluid (e.g. liquid or emulsion) or a solid substance dissolved in the fluid or suspended in a fluid environment.

The preferred item 40 processing for use in accordance with the variants of implementation of the present invention is an automated injection system INOVOJECT® (Embrex, Inc., Research Triangle Park, North Carolina (North Carolina)). However, suitable for use in accordance with the variants of implementation of the present invention is any device injection in ovo capable podshoev is taken, as described here, to the control device. Suitable injection device is preferably designed to work in conjunction with commercial devices accessory trucks or strips for eggs, examples of which are described above.

On Fig-51 illustrates sorting and moving eggs 1' until further processing in accordance with the variants of implementation of the present invention. According pig point 500 sorting includes a closed loop conveyor system 502 and a pair of moving heads, 504, 506, operatively associated with it. Eggs with identified characteristics (e.g., floors) are placed on a conveyor system 502 at one end a gasket or other holding containers and move along the conveyor system in the direction indicated by the arrow A8. Moving head 504 includes a matrix of suction cups 137, as described above with reference to Fig-29, which are designed for simultaneous uplift of the many eggs from the conveyor system 502 and placed the eggs on the first conveyor belt 508 (Fig). Moving head 506 includes a matrix of suction cups 137 which are adapted for simultaneous uplift of the many eggs from the conveyor system 501 and placed the eggs on the second conveyor belt 510 (Fig).

Each moving head 504, 506 may be which is designed to selectively lifting the eggs from the conveyor system 502 based on the characteristics of the eggs (e.g., the floor). For example, moving the head 504 may be intended only for lifting the eggs of males, while moving the head 506 is designed for lifting only the eggs of females. Moving heads 504, 506 and conveyor system 502 is preferably controlled by the computer (for example, PLC 70C on Fig).

As shown in Fig moving head 504, 506 are designed to move in the direction indicated by the arrows And9so that eggs can be interfered with on the appropriate conveyor belt 508, 510. The direction of movement of conveyor belt 508, 510 is also indicated by the arrows A9.

According pig each conveyor belt 508, 510 operatively associated with the respective device 520 bookmarks. Each device 520 bookmarks designed to guide and maintain the eggs in a predetermined position for processing (for example, injection etc). Each illustrated device 520 bookmark includes a closed conveyor 522, having a multitude of parallel rollers 524, which are rotating way connected with their ends to the drive mechanism (for example, chains etc). Rollers 524 move in the direction indicated by the arrows And9while rotation in the clockwise direction, as seen in Fig. Under the action of translations and rotations of rollers 524 eggs 1' on Igusa in the direction the arrow And9(with their narrow ends, generally perpendicular to the direction of the arrow And9and entered in the corresponding channels 528, and then into the corresponding receiving Cup 530, with their narrow ends facing down, as shown in Fig. The receiving Cup 530 is installed on a closed conveyor system 540, which moves the Cup in the direction indicated by arrows A9. The example device 520 bookmarks are described in U.S. patent No. 3592327.

Each receiving Cup 530 transfers the corresponding egg 1' in paragraph 40 of the processing type of the automated injection system INOVOJECT®. For example, in the illustrated on Fig embodiment eggs 1' within the corresponding receiving cups 530 transported through the relevant paragraphs 40, 50 of the handle and move around. Each paragraph 40 of the handle contains a set of drawers injections, which are designed for the introduction of substances into eggs 1'. Paragraph 50 move installed downstream of each paragraph 40 of the handle and adapted to move eggs 1' in the appropriate containers (not shown).

The device of the bookmark in accordance with the variants of implementation of the present invention may have various configurations and is not limited to the illustrated implementation options. The device can bookmark with erati different number of channels and may include receiving cups of various sizes and/or configurations. In addition, you can use different types of rollers and conveyor systems without restrictions.

On Fig illustrates the points 40, 50 processing and sorting/moving according to other variants of implementation of the present invention. When the gasket 7 eggs 1' after the sample is transported through the point 43 processing control device 20 (11) selectively produces the signal injection point 40 treatment to those injected eggs 1'which have been identified as having a particular characteristic. As should be obvious to experts in the art, the generation of a selective signal injection can be obtained using different approaches, including the generation of a signal, which causes selected to be injected eggs, or the generation of a signal that does not allow injecting not selected eggs.

In the illustrated embodiment uses a pair of injection points 41, 42, such as an automated injection system INOVOJECT®. The first injection point 41 contains the first set of drawers injections, which are designed for the introduction of substances into eggs, 1', is identified as having the first characteristic. The second injection point 42 contains the first set of drawers injections which are adapted to put what I substances in eggs 1', identified as having the second characteristic. For example, if the identified feature is a floor, the first injection point 41 can administer the vaccine or other substance in eggs of males, and the second injection point 42 can administer the vaccine or other substance in eggs of females.

Paragraph 50 of the sorting/moving can be installed downstream relative to paragraph 40 of the processing. The control unit 20 generates a selective signal removal, to make a point 50 sort/move to remove the eggs that have identified various characteristics (e.g. gender). Paragraph 50 of the sort/move can use the lifting device of the suction type, as described above relative to the lifting heads 132, 134 of the device 30 of the extraction material. You can also use any other appropriate means for removing eggs, such devices are well-known specialists in this field of technology.

In the illustrated embodiment, sorted eggs identified in accordance with the floor. The eggs of males suffer with pads 7 for eggs in appropriate containers 51 and eggs females carry with pads 7 for eggs in appropriate containers 52. Any inanimate eggs can be left in the pads 7 for eggs for further processing or removal.

Paragraph 50 of the sorting/p the motion preferably operates automatically and robotvision. Alternatively, selected eggs can be identified in the device 22 pairing operator with the system, the optional label and delete it manually.

In accordance with the variants of implementation of the present invention, the eggs can be sorted on the basis of viability, the content of pathogenic organisms and/or genetic analysis. For example, eggs, which contain disease-causing microorganisms can be removed from normalna together and do not transfer to the incubator, thus preventing horizontal transmission of disease agents.

System in accordance with the variants of implementation of the present invention can provide valuable information for those engaged in poultry production on an industrial basis. For example, the identification and compilation (compilation) classes embryonic mortality can provide feedback on the management of the breeding herd, the terms of manipulation and incubation of eggs. Knowledge of the number of viable eggs and sex can provide accurate prediction of the result and to rationalize and optimize logistics. Identification identification of pathogenic organisms and compile data can help in treating the disease. Breeders can use the identification of genetic markers. Identification of pittel the different elements inside the egg can be used for optimization of feed rations and feeding regimes. The identification of proteins or small molecules can be used for tracking or prediction or optimization of productivity or immunity. In addition, you can use the information from the embodiments of the present invention for tracking components eggs and then linking them with the productivity of birds and to use this information to develop new products.

In accordance with the variants of implementation of the present invention can be constructed item retrieval of material to perform various methods of analysis to determine characteristics of the eggs. Fig-54 illustrate the module 600, which is intended for connection to the device 30 of the extraction material Fig. Approximate module 600 for analyzing material in accordance with the variants of implementation of the present invention and, in particular, using the procedure described below, the competitive analysis of antibodies produced by the Corporation Luminex Corporation, Austin (Austin), Texas.

The instantiated module 600 is designed to extract small samples of material extracted from eggs, from the respective vessels for samples and feed them into the system reader for analysis. The tray 151 for samples with many vessels 152 for samples that contain material extracted from eggs, preferably BB which is illustrated in module 600 of the system 150 manipulating trays for samples.

The procedure of competitive analysis antibodies used in the module 600 and based on the antibody associated with painted inside "granules". The instantiated module 600 may be adapted to manipulate at the same time any number of trays 151 for samples. For each tray 151 for samples module 600 includes a pointing device for liquids, which is designed to extract small samples of the respective vessels for samples in the tray 151 for samples and feed them into the system reader.

In particular, if the material that was extracted from eggs, is allantoin fluid module 600 takes allantoin liquid and mixes it with microspheres of polystyrene or pellets (supplied in Luminex, Inc., Austin, Texas)that are associated with molecules of estradiol. To the mixture of granules/allantoin liquid add labeled using fluorescence antibody of antisdruciolo and mix. Then this mixture is incubated at room temperature in the dark for 15-30 minutes. Some amount of the mixture (for example, 50 - 60 µl) of the extract and the results of the analysis provide analyzer (Luminex, Inc., Austin, Texas), in which to determine the fluorescence signal of laser are used.

This procedure tests based on competitive inhibition. Competition for labeled and with the use of fluorescence antibody antisdruciolo is established between estradiol, associated with the granules, and estradiol in the sample of the allantois. If the sample allantois obtained from embryo females and contains estradiol, estradiol in the sample competes for labeled using fluorescence antibody, and the granules will involve a lesser amount of antibodies. Signal analysis, depending on the number of antibodies bound to the granules will be lower from the sample obtained from females (inhibition (suppression) of fluorescent signal). If the sample allantois obtained from embryo is male and does not contain estradiol, competition from estradiol in the sample is much smaller and more pellets will be bound antibody. The more antibodies bound to the granules, the better the signal.

In accordance with the variants of implementation of the present invention associated granules and the antibody may be already present inside the blood vessels to the sample tray of samples. By eliminating the extra steps add pellets and antibodies extracted with allantoine fluid-time analysis can be reduced, which may be advantageous from a commercial point of view.

According pig illustrated module 600 includes a system 602 manipulating patterns, high-performance system 604 readout for the analysis of samples, the control unit 606 and the system 608 supply and discharge of the fluid./p>

The above description is illustrative for the present invention and should not be construed as limiting thereof. Although there have been described several examples of embodiments of this invention, specialists in the art can easily understand that these options exercise can do a lot of modification without material departure from the new provisions and without loss of the advantages of this invention. Accordingly, all such modifications are intended to include in the scope of this invention as defined in the claims. Therefore it should be understood that the above description is illustrative for the present invention and should not be construed as limited to the specific disclosed variants of implementation and that modifications of the disclosed embodiments, as well as other embodiments of, intended for inclusion in the scope of the claims appended claims. The invention defined in the subsequent claims, with equivalents of the claims to be included in it.

1. The method of processing eggs, having an identifiable characteristic, which remove material from each of multiple eggs, analyze the material extracted from each egg to identify eggs having PR is the sign, and selectively process the eggs identified as having this feature.

2. The method according to claim 1, which additionally identify live eggs among the eggs before retrieving material and extract material from eggs identified as live eggs.

3. The method according to claim 2, wherein identifying live eggs includes Ovasapyan eggs.

4. The method according to claim 2, wherein to identify live eggs cover each egg with light from a light source, and light includes light at wavelengths in the visible and in the infrared range, take the light passing through each egg, in the detector, installed next to each egg, determine the light intensity received at the selected one of the wavelengths in the visible and infrared ranges for each egg, generate a spectrum for each egg, which displays the intensity of light at multiple wavelengths of visible and infrared ranges, and comparing the generated spectrum for each egg with a spectrum associated with a live egg, to identify live eggs.

5. The method according to claim 4, wherein when the lighting of each egg with light cover each egg with light at wavelengths in the range from approximately 300 nm to approximately 1100 nm.

6. The method according to claim 2, wherein when identifying live eggs measure the opacity of the eggs, measure the temperature of the eggs, and the IDA is difficiult live eggs, using measured values of opacity and temperature.

7. The method according to claim 1, wherein when removing material from the eggs extracted from eggs allantoin fluid, amnion, yolk, shell, fiber, fabric embryo Solopova shell and/or blood.

8. The method according to claim 7, wherein when removing material from the eggs have each egg, essentially in a horizontal orientation, the probe is introduced into each egg through the shell and extract the sample allantoine liquid from the allantois of each egg through each probe.

9. The method of claim 8, wherein the location of each egg, essentially in a horizontal orientation includes the location of each egg so that the longitudinal axis of each egg is oriented at an angle between approximately 10 degrees and approximately 180 degrees from the vertical, and the vertical angle of zero degrees is defined by the blunt end of the egg, which is directed vertically upward position.

10. The method of claim 8, wherein optionally change the position of each of the eggs from the essentially horizontal orientation to an essentially vertical orientation after allatoona liquid derived from it, and move essentially vertically oriented egg in another location.

11. The method of claim 8, wherein the analyzing of the material extracted from each of the eggs is, to identify one or more characteristics of each egg includes detection of the presence of estrogenic composition in the extracted allantoine liquid.

12. The method according to claim 11, wherein when identifying the presence of estrogenic composition distribute allantoin liquid extracted from eggs, in appropriate vessels, distribute biosensor in the vessels, and the biosensor is designed to chemically interact with estrogenic composition in allantoine fluid and change the color signal allantoine fluid, and detect signal changes color allantoine the fluid inside the vessels.

13. The method according to claim 11, wherein when identifying the presence of estrogenic composition distribute allantoin liquid extracted from eggs, in appropriate vessels, each vessel contains a biosensor, is designed to chemically interact with estrogenic composition in allantoine fluid and change the color signal allantoine fluid, and detect signal changes color allantoine the fluid inside the vessels.

14. The method according to claim 1, wherein the analyzing of the material extracted from each egg to identify one or more characteristics of each egg includes identifying the sex of each egg and in which the selective processing of live eggs includes selective introduction of HAC the ins eggs of a certain sex.

15. The method according to item 13, wherein optionally enter the first vaccine in eggs identified as male eggs, and enter the second vaccine in eggs identified as the eggs of the female sex.

16. The method according to claim 1, wherein to analyze the material extracted from each egg to identify one or more characteristics of each egg to identify the sex of each egg and wherein in the processing of live eggs take eggs identified as having the same gender.

17. The method according to claim 1, wherein to analyze the material extracted from each egg to identify one or more characteristics of each egg to identify one or more pathogens inside each egg and wherein in the processing of live eggs removing eggs identified as having one or more pathogens.

18. The method according to claim 1, wherein when analyzing the material extracted from each egg to identify one or more characteristics of each of the eggs perform genetic analysis on each egg.

19. The method of processing eggs, having an identifiable characteristic, which is the floor at which identify live eggs among many eggs, remove material from eggs identified as live eggs, analyze the material extracted from each of the living eggs for ID is tificatio each floor of the living eggs and selectively injected vaccines in live eggs in accordance with the floor.

20. The method according to claim 19, which further sorted live eggs in accordance with the floor to selective introduction of vaccines in live eggs.

21. The method according to claim 19, which further sorted live eggs in accordance with the floor after selective injection of vaccines in live eggs.

22. The method according to claim 19, wherein when identifying live eggs will otoscopic eggs.

23. The method according to claim 19, wherein when identifying live eggs cover each egg with light from a light source in which light includes light at wavelengths in the visible and infrared ranges, take the light passing through each egg, in the detector, installed next to each egg, determine the light intensity received at the selected one of the wavelengths of visible and infrared ranges for each egg, generate a spectrum for each egg, which displays the intensity of light at multiple wavelengths of visible and infrared ranges, and comparing the generated spectrum for each egg with spectrum associated with a live egg, to identify live eggs.

24. The method according to claim 19, wherein when the lighting of each egg with light cover each egg with light at wavelengths from about 300 nm to about 1100 nm.

25. The method according to claim 19, wherein when identifying live eggs measure opacity sets the eggs, measure the temperature of many eggs and identify live eggs, using measured values of opacity and temperature.

26. The method according to claim 19, wherein when removing material from the egg extract allantoin fluid, amnion, yolk, shell, protein, fetal tissue, Solopova shell and/or the blood of the eggs.

27. The method according to p, wherein when analyzing the material extracted from each of the living eggs, for identifying the sex of every living eggs determine the presence of estrogenic composition in allantoine fluid extracted from each of the living eggs.

28. The method according to p, wherein when removing material from the eggs have each of fresh eggs, essentially in a horizontal orientation, the probe is introduced into each egg through the shell and extract the sample allantoine liquid from the allantois of each egg through each probe.

29. The method according to p, wherein when the location of each living eggs in essentially horizontal orientation feature each of live eggs in such a way that the longitudinal axis of each egg is oriented at an angle between approximately 10 degrees and approximately 180 degrees from the vertical, and the vertical angle of zero degrees is defined by the blunt end of the egg, which is directed vertically upward position.

30. The method according to p in which optionally change aout the position of each of the live eggs from, essentially horizontal orientation to an essentially vertical orientation after allatoona liquid from them extracted, and move essentially vertically oriented live eggs to another location.

31. The method according to item 27, wherein when determining the presence of estrogenic composition distribute allantoin liquid, extracted from living eggs into appropriate vessels, distribute biosensor in the vessels, and the biosensor is selected in such a way that chemically interact with estrogenic composition in allantoine fluid and change the color signal allantoine fluid, and detect signal changes color allantoine the fluid inside the vessels.

32. The method according to item 27, wherein when determining the presence of estrogenic composition distribute allantoin liquid extracted from eggs, in appropriate vessels, each vessel contains a biosensor, is designed to chemically interact with estrogenic composition in allantoine fluid and change the color allantoine fluid, and detect signal changes color allantoine the fluid inside the vessels.

33. The method according to claim 19, wherein when determining the presence of estrogenic composition distribute allantoin liquid extracted from eggs, in appropriate vessels, each vessel contains the BIOS is nsor, designed for chemical interaction with estrogenic composition in allantoine fluid, and produce a detectable signal, and detect a signal produced within one or more vessels.

34. The method of processing eggs, having an identifiable characteristic, which is the floor at which identify live eggs among the eggs, remove allantoine the liquid from the eggs identified as live eggs, thus have every one of the living eggs, essentially in a horizontal orientation, whereby the allantois of each egg is combined and increases allantoine bag under the top part of the shell of each egg, the probe is introduced into each egg through the shell of the egg and directly into the enlarged allantoine bag and extract the sample allantoine liquid from the allantois of each egg through each probe, to detect the presence of estrogenic composition in allantoine liquid extracted from every living eggs, to identify each floor of the living eggs, assigning allantoin liquid, extracted from living eggs into appropriate vessels, distribute biosensor in the vessels, and the biosensor is selected so that chemically interact with estrogenic composition in allantoine fluid and change the color signal allantoine fluid, and to detect changes color signal al is antinoi the fluid inside the vessels, and selectively injected the vaccine into live eggs in accordance with the floor.

35. The method according to clause 34, which further sorted live eggs in accordance with the floor.

36. The method according to clause 34, wherein when identifying live eggs will otoscopic each egg.

37. The method according to clause 34, wherein when the selective introduction of a vaccine in the living eggs of the same sex enter the first vaccine live eggs identified as male eggs, and enter the second vaccine live eggs identified as the eggs of the female sex.

38. The method according to clause 34, wherein when the selective introduction of a vaccine in living eggs in accordance with the floor injected the vaccine into live eggs identified as having the same gender.

39. The method of processing eggs, having an identifiable characteristic, which is the pathogenic microorganisms which remove material from each of the eggs, analyze the material extracted from each egg to identify eggs having one or more pathogens, and remove the eggs identified as having one or more pathogens.

40. The method according to § 39, which further identifies live eggs among the eggs, and extract material from eggs identified as live eggs.

41. The method of processing eggs, having an identifiable characteristic, which is BALTNET the priori microorganisms, at which remove material from each of the eggs, analyze the material extracted from each egg to identify the sex of each egg, analyze the material extracted from each egg to identify eggs having one or more pathogens, and remove the eggs identified as having one or more pathogens.

42. The method according to paragraph 41, wherein additionally identify live eggs among the eggs before retrieving material and extract material from eggs identified as live eggs.

43. The method according to paragraph 41, wherein optionally selectively injected vaccine in eggs according to their sex.

44. The method according to item 43, wherein optionally enter the first vaccine in eggs identified as male eggs, and enter the second vaccine in eggs identified as the eggs of the female sex.

45. The method according to paragraph 41, wherein optionally remove eggs identified as eggs, have the same floor.

46. The method of processing eggs, having an identifiable characteristic, which is the pathogenic microorganisms which remove material from each of the eggs, carry out genetic analysis of material extracted from each of the eggs, analyze the material extracted from each egg to identify the sex of each egg, analyze the material, sveceny from each of the eggs, to identify eggs having one or more pathogens, and remove the eggs identified as having one or more pathogens.

47. The method according to item 46, wherein additionally identify live eggs among the eggs before retrieving material and extract material from eggs identified as live eggs.

48. The method according to item 46, wherein optionally selectively injected vaccine in eggs according to their sex.

49. The method according to p, which additionally is selectively injected vaccines in eggs according to their sex.

50. The method according to item 46, wherein optionally remove eggs, identificirane as eggs of the same sex.

51. The method of processing eggs, having an identifiable characteristic, which is the pathogenic microorganisms which remove material from each of the eggs, carry out genetic analysis of material extracted from each of the eggs, analyze the material extracted from each egg to identify eggs having one or more pathogens, and remove the eggs identified as having one or more pathogens.

52. The method according to § 51, which further identifies live eggs among the eggs before retrieving material and extract material from living eggs.

53. The way obrabotke, having an identifiable characteristic, which is the floor at which remove material from each of the eggs, carry out genetic analysis of material extracted from each of the eggs, analyze the material extracted from each egg to identify the sex of each egg.

54. The method according to item 53, wherein additionally identify live eggs among the eggs before retrieving material and extract material from eggs identified as live eggs.

55. The method according to item 53, wherein optionally selectively injected vaccine in eggs according to their sex.

56. The method according to § 55, at which optionally enter the first vaccine in eggs identified as male eggs, and enter the second vaccine in eggs identified as the eggs of the female sex.

57. The method according to § 55, at which optionally remove eggs with the same gender.

58. The method of processing eggs, having an identifiable characteristic, which is the genetic traits, which identifies live eggs among the eggs, remove the material from eggs identified as live eggs, and conduct genetic analysis of material extracted from each of the eggs.



 

Same patents:

FIELD: poultry industry.

SUBSTANCE: claimed method includes treatment of incubating egg shell with mitomin in concentration of 0.3-0.5 % and in amount of 2.2-2.5 ml/120-150 eggs.

EFFECT: increased egg hatching, decreased mortality; daily chickens with improved biochemical blood characteristics.

5 ex, 5 tbl

FIELD: poultry science.

SUBSTANCE: incubation eggs should be once treated with ultraviolet lamp for 20 sec or ozonized, then sprayed with 0.01-0.3%-succinic acid solution at 1.2-1.5 ml/120-150 eggs to increased the brood in chickens, decrease lethality and increase body weight in chickens.

EFFECT: higher efficiency of stimulation.

6 ex, 6 tbl

FIELD: poultry science.

SUBSTANCE: one should irradiate eggs before incubation with the light of He-Ne laser LHN-104, wave length being 632.8 nm, power density at eggs surface being 50 mW/sq.cm/sec, with red light of fluorescent lamp DNESG-500, wave length being 630-650 nm, power at eggs surface being 23.1 erg and with ultraviolet from DRT-400 lamp at wave length being 185-400 nm, average dosage at eggs surface being 20 merg in 3-min-long exposures.

EFFECT: higher resistance to pullorosis in poultry.

4 dwg, 4 tbl

The invention relates to agriculture, in particular to poultry

The invention relates to poultry

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The invention relates to poultry and veterinary medicine and can be used as an additional component of stimulation hatchability of Chicks in the incubator

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The invention relates to agriculture, namely the industrial poultry to poultry meat breeds

FIELD: poultry science.

SUBSTANCE: one should irradiate eggs before incubation with the light of He-Ne laser LHN-104, wave length being 632.8 nm, power density at eggs surface being 50 mW/sq.cm/sec, with red light of fluorescent lamp DNESG-500, wave length being 630-650 nm, power at eggs surface being 23.1 erg and with ultraviolet from DRT-400 lamp at wave length being 185-400 nm, average dosage at eggs surface being 20 merg in 3-min-long exposures.

EFFECT: higher resistance to pullorosis in poultry.

4 dwg, 4 tbl

FIELD: poultry science.

SUBSTANCE: incubation eggs should be once treated with ultraviolet lamp for 20 sec or ozonized, then sprayed with 0.01-0.3%-succinic acid solution at 1.2-1.5 ml/120-150 eggs to increased the brood in chickens, decrease lethality and increase body weight in chickens.

EFFECT: higher efficiency of stimulation.

6 ex, 6 tbl

FIELD: poultry industry.

SUBSTANCE: claimed method includes treatment of incubating egg shell with mitomin in concentration of 0.3-0.5 % and in amount of 2.2-2.5 ml/120-150 eggs.

EFFECT: increased egg hatching, decreased mortality; daily chickens with improved biochemical blood characteristics.

5 ex, 5 tbl

FIELD: poultry farming.

SUBSTANCE: method involves withdrawing material from multiplicity of live eggs; analyzing withdrawn material for identifying of eggs having predetermined characteristic; treating in proper manner.

EFFECT: increased efficiency by providing the possibility of analyzing withdrawn material and performing proper treatment.

58 cl, 54 dwg

FIELD: poultry farming.

SUBSTANCE: incubation eggs are treated with aqueous solution of edible salt, with sea salt being additionally introduced, said components being used in the following ratio, g/l: NaCl 2.5-3.5; sea salt 0.3-0.5. Method involves preparing aqueous solution of edible salt by double distillation of sodium chloride solution and adding sea salt during repeated distillation procedure; treating incubation eggs by aerosol applying process, with following exposing for 30-60 min.

EFFECT: increased efficiency in treatment of eggs.

3 cl, 1 tbl, 1 ex

Laser beams scanner // 2265997

FIELD: optics.

SUBSTANCE: scanner has mirrors. Ten mirrors are mounted onto truncated cone of scanner at different angles to light source for scanning laser beams at direction of lightening chamber and for increase in exposure of processing.

EFFECT: reduced electric energy consumption.

1 dwg

FIELD: radiation technology.

SUBSTANCE: unit has gas-discharge lamp ДНЕСГ-500, helium-neon laser ЛГН-104, ultraviolet lamp ДРТ-400, germicidal lamps БУВ-15. Germicidal lamps БУВ-30 are placed in cassette of optical filter of gas-discharge lamp ДНЕСГ-500 and under transporting mechanism for irradiation of total surfaces of incubation eggs to improve germicidal effect.

EFFECT: improved quality of disinfection; improved farming-biological efficiency.

3 dwg

FIELD: optics; laser engineering.

SUBSTANCE: assembly has gas-discharge lamp ДНЕСГ-500, helium-neon laser ЛГН-104, mercury-quartz lamp ДРТ-400 and germicidal lamps БУВ-15 as well as cassettes of optical filters of lamps ДНЕСГ--500, ДРТ-400, БУВ-15. There are also three germicidal lamps БУВ-30 disposed at angle of 120° to incubation eggs and IR laser "Uzor" of cassette of optical filter of lamp БУВ-30. Cassettes of optical filters of lamps ДНЕСГ-500, БУВ-30, ДРТ-400, БУВ-15 are decompressed at the direction of radiant chamber for complex influence onto incubation eggs with laser beams, ozone and ionized air and for disinfecting eggs at all the sides.

EFFECT: reduced energy consumption; improved productivity; higher vital capacity.

5 dwg

FIELD: agricultural engineering.

SUBSTANCE: installation has gas discharge lamp, mercury-quartz lamp, germicidal lamps, and helium-neon laser. Installation is furnished with infrared laser for deep heating of incubation eggs by way of multiple-mode pulsed laser radiation of proximate infrared spectral region.

EFFECT: increased vitality and productivity of birds.

2 dwg

FIELD: agricultural engineering.

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EFFECT: increased efficiency in protecting of lightening equipment from settling of dust thereon and reduced consumption of power.

2 dwg

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