Method for preparing complex immune modulator

FIELD: veterinary science.

SUBSTANCE: the present innovation deals with cooling selected fertilized uninfected eggs at 2-4 C up to 7 d followed by homogenization, diluting with physiological solution of egg mass, bactericidal treatment, conservation with phenol solution up to its concentration being 0.3% at subsequent filtration of target product. Moreover, egg mass should be diluted with physiological solution at the ratio of 1:5, then comes thermal treatment upon water bath at 70-80 C for 1 h and before conservation one should cool the target product in bactericidal chamber under the lamp with ultraviolet irradiation for 30-40 min. The method provides increased total and specific body resistance, it enables to obtain cheaper and more acceptable biostimulant and simplified way for preparing.

EFFECT: higher efficiency.

4 ex, 3 tbl

 

The technical field to which the invention relates.

The invention relates to veterinary medicine, in particular to medicines to improve General and specific resistance of the organism of animals by applying a non-specific stimulant drugs have regulatory effects on the immune system - the immune modulators.

The level of technology

Known biostimulant-placenta, the active principle from the placenta of women. The drug is manufactured by the new technology, without heat treatment using the sterilization effect of cavitation and the newly discovered chemical reagents (see the New generation of tissue preparations - PAN// Theoretical and practical aspects of the emergence and development of animal disease and protection of their health in modern conditions". The international conference. Voronezh, October 3-4, 2002, conference proceedings. - Volume 1. - Voronezh, 2000. - 295 S.).

The disadvantage of this method of preparation of the biostimulator is the high cost, complexity and multi-stage cooking.

There is also known a method of preparation of biostimulant for poultry feeding, providing an introduction to the basic diet of the biostimulator, prepared by cooling at a temperature of 2-4°C for 4-5 days, dilution with water five times the volume and sedimentation during the day followed and what carlaviruses obtained mass at the same time as the biostimulant use egg, and then introduce it in the diet in quantities of 0.2-0.3 ml per 1 kg body weight every 6-7 days (see A.S. USSR №150163, class. And 23 To 1/16).

The disadvantage of this method of cooking biostimulant for feeding birds is not sufficiently high effect of improving the General and the specific resistance of the organism.

The closest in technical essence and the achieved positive effect to the claimed method is "a Method of preparation of the biostimulator embryonic (Stambha)". The method consists in the following. Carry out selection of fresh uninfected eggs, then incubated for 9 days at 5, 6, 7 day eggs irradiated for 30 seconds at a modulation frequency of 9 Hz and radiation power of 9 mW, then the eggs cool for 6-7 days, spend homogenization, diluted egg mass 1:10 saline solution, mix thoroughly, add to the mixture of 5% phenol solution so that the concentration of phenol was 0.3%, the resulting solution was filtered and poured into sterile vials, closed and stored in a refrigerator at a temperature of 2-6°With (patent 2197251 RF, MKI 7 a 61 K 35/12).

The disadvantage of this method is the high cost of materials, complexity, and multiple, high energy costs and the cost of preparation is the same.

Disclosure of inventions

The aim of the invention is to create a comprehensive immune modulator (KIM), which helps to intensify the treatment and prevention of a number of diseases and pathological conditions of animals, accompanied by a decrease of immunobiological properties of the body, and provides cooking cheap and effective immunomodulator, in which the substrate includes a fertilized chicken egg. Used rational methods, aimed at preserving the original properties of the substrate, and obtaining a number of biologically active substances.

The technical result that can be achieved using the proposed method, is to increase the General and the specific resistance of the organism, getting more cheap and available biostimulant, simplification of the method of preparation. The technical result is achieved by using the method of preparation of the complex immune modulator, comprising cooling the selected fertilized uninfected eggs at a temperature of 2-4°up to 7 days, homogenization, dilution with saline egg masses, bactericidal treatment, the preservation solution of phenol to its concentration of 0.3%, followed by filtration of the target product, characterized in that the egg mass is diluted with ideologicheskim solution in the ratio 1:5, then conduct heat treatment in a water bath at a temperature of 70-80°C, within 1 hour, and before conservation target product is cooled in a germicidal chamber under a lamp with UV irradiation for 30-40 minutes.

The essence of the proposed method

In the first stage, eggs are taken from successful in infectious diseases management. Use eggs of medium size, about the same weight, no visible damage and contamination of the shell. In the second stage, place eggs in a dark cold room at a temperature of 2-4°With 5-7 days, which leads to the slow death of the embryo to the formulation of nutrients in adverse conditions. Remove eggs from refrigerator compartment, open, inspect the yolk for the presence of the embryo, note volume, hold homogenization. The resulting homogenized diluted with 0.9% saline solution in a ratio of 1:5. Maintain the mixture in a water bath at a temperature of 70-80°C for 1 hour. Homogenized placed in a germicidal chamber and incubated for 30-40 minutes. Then filtered and poured into sterile vials. For conservation using 0.5% phenol solution until its concentration of 0.3%.

The implementation of the invention

Examples of specific performance of the proposed method

Example 1. At the first stage eggs on beraut of successful in infectious diseases management. Use eggs of medium size, about the same weight, no visible damage and contamination of the shell. Cooling selected fertilized uninfected eggs at a temperature of 2-4°C for 2-3 days, homogenization, dilution with saline egg masses in the ratio 1:3, heat treatment in an autoclave at a temperature of 120°and a pressure of 1.5 ATM. If the shutter speed in the refrigerator for 2-3 days after the heat treatment of the mass in the autoclave, the mass of curled, was not fit for further use.

Example 2. In the first stage, eggs are taken from successful in infectious diseases management. Use eggs of medium size, about the same weight, no visible damage and contamination of the shell. Cooling selected fertilized uninfected eggs at a temperature of 2-4°C for 5-7 days, homogenization, dilution with saline egg masses in the ratio 1:3, heat treatment in a water bath at a temperature of 70-80°C, within 1 hour, cooled in a germicidal chamber under a lamp with UV irradiation for 40 minutes. Conservation is carried out with 0.5%phenol solution until its concentration is 0.1% of the mass fraction of the egg mass. A number of biologically active substances in the resulting preparation was very low TC. egg white folded and left on the filter. The concentration of phenol is low, the crops on the positive sterility.

Example 3. In the first stage, eggs are taken from successful in infectious diseases management. Use eggs of medium size, about the same weight, no visible damage and contamination of the shell. This method includes cooling the selected fertilized uninfected eggs at a temperature of 2-4°up to 7 days, homogenization, dilution with saline egg masses, bactericidal treatment, the preservation solution of phenol to its concentration of 0.3%, followed by filtration of the target product, characterized in that the egg mass is diluted with a physiological solution at a ratio of 1:5, and then conducting heat treatment in a water bath at a temperature of 70-80°C, within 1 hour, and before conservation target product is cooled in a germicidal chamber under a lamp with UV irradiation for 30-40 minutes.

This method is most efficient, as the concentration of active substances is the greatest, the drug is safe for a given concentration of the preservative when used on animals.

Example 4. In the first stage, eggs are taken from successful in infectious diseases management. Use eggs of medium size, about the same weight, without the of dimych damage and contamination of the shell. The difference between this method is to cool selected fertilized uninfected eggs at a temperature of 2-4°up to 7 days, homogenization, dilution with saline egg mass in a ratio of 1:7, thermal, bactericidal treatment, filtration. thermal treatment is carried out in a water bath at a temperature of 70-80°C, for one hour, cooled in a germicidal chamber under a lamp with UV irradiation for 30-40 minutes, conservation is carried out with 0.5% phenol solution until its concentration of 1%, and filtering.

With the introduction of this drug in animals, the injection sites appeared infiltrates, the drug showed no irritant effect on the skin of the animal, due to the high concentration of phenol. As a result of lower concentrations of the drug, its effectiveness has decreased markedly.

The results of the test drug, KIM animals

In the manufacture of an experimental batch conducted a biological sample on its harmlessness. Test conducted on laboratory mice and rabbits.

The results of the test drug KIM on laboratory animals

Development and application of drugs and bio-stimulants used to improve immunobiological reactivity in animals as prevention and as part of various therapeutic schemes are with resnow task.

Biological testing of the drug was carried out in the laboratory on white mice. Were used in the experiment two groups of five mice: the first group was experienced, and the second served as a control. Animals were housed under identical conditions and feeding. Weighing of animals were carried out: before putting on the experience and every two days. Monitoring a physiological condition of the animals was carried out daily. Mouse experimental group was inheritability drug (KIM) at a dose of 0.5 ml/bird. The weighing results we obtained the following results: in the experimental group received increases in body weight, on average, 25% more than in animals of the control group. The results of the use of the drug KIM on the mink are given in table 1.

20
Table 1

The results of the drug KIM on the mink
Age and sex groupNumber of goalsDose, mg/kgMultiplicity ofNote
Adult animals:

Males


20


0,1


3-fold h/C 3days
- improvement of the appetite; the increase in the number of coatings during heat and rapid recovery after him; improving the quality of sperm.
Female0,13-fold h/C 3 days- reduction of cases of cannibalism; improving lactation; improving the safety of young and jigging; rapid recovery of the females after lactational depletion
Young:

Newborn


20


0.01 ml/goal


3-fold h/C 3 days
notes a good appetite; increase hurry masses; fast

schooling to independent feeding.
30-45 days200.05 ml/kg3-fold h/C 3 daysnotes a good appetite; increased body weight; rapid habituation to independent feeding.
45-60 days200.05 ml/kg3-fold h/C 3 daysnotes a good appetite; increased body weight; rapid habituation to self-supply; reduction of cannibalism; reduced body response to vaccination.

Research conducted on tribal enterprise "red guard" of Stavropol Krai in 2003 suggest that drug use KIM in a dose of 0.05 ml per 1 kg body weight three times in 7 days promotes an increase in blood boars producing hemoglobin 633 g/l, bactericidal activity of blood serum to 5.75%, lysozyme activity of serum of 3.97%. Increased activity of transamination enzymes ALT and ACT 0.07 and 0.08 µkat/l, alkalinity about 4.4%2the content of total protein in the blood 10.2 g/l, content γ-globulin on 6,27% compared with the control group.

The study of biochemical parameters of blood mink showed that the amount of hemoglobin in males of the experimental group, which used complex immunomodulator KIM, was greater than that of counterparts in the control group was 4.7 g/l For total protein content in serum was also the advantage of males, where medication used KIM to 3.5 g/L. On albumen fraction differences between groups were in favor of the animals of the experimental group. Gamma-globulin fraction of the animals of the experimental group outperformed the control by 1.8%.

All of the above speaks to the increasing resistance of boars manufacturers and male mink, and immunomoduliruyushhih properties of the drug, KIM.

Biochemical analysis of the drug is important as an indicator of their quality. According to many scientists, a number of compounds has a high biological potential and can cause major bio-stimulating function of the drug. These substances primarily amino acids. Aminoxy the pilot is part of the preparation are shown in table 2.

Table 2

The amino acid composition of the drug, KIM
Amino acidsMg %
Interchangeable
Aspartic acidof 5.4
Serine3,4
Glutamic acid10,0
Alanine7,6
Terezin3,2
Only29,6
Irreplaceable
Trianonthe 3.8
Glycinea 4.9
Valine6,2
Metionin1,5
Isoleucine5,2
Leucine9,2
Phenylalanine5,2
Histidine3,7
Lysine3,3
Arginine4,0
Only47,0

To characterize the biological value of this drug are such concept as amino acid index, which determines the amino acid usefulness, it is equal to the ratio of essential amino acids to interchangeable. In biologically valuable in the societies he is above 1, amino acid index (Kim)=47,0/29,6=1,59.

In preparation KIM contains the following hormones (table 3)

Table 3

The content of hormones in the medication KIM
Name the hormonesNumber
TSH (thyroid stimulating hormone), mmed/l0,09
FSH (follicle stimulating hormone), mIU/ml1,5
Free T4 (L-thyroxine), pmol/l0,2
LH (luteinising hormone), mIU/ml2,2
Progesterone, nmol/l>100,0
Testosterone, nmol/l5,8
Cornisa, nmol/l0,1
Prolactin, mIU/ml75,0
STH (somatotropic hormone), mmed/l10,0
DHEAS (dehydroepiandrosterone), µg/ml0,26
ACTH (adrenocorticotropic hormone), PG/mg0,9

The quantitative composition of the drug

100 ml of the preparation contains:

20 ml of biologically active egg mass;

- 79,7 ml of sodium chloride 0.9 per cent;

to 0.3 ml of phenol as preservative.

This drug is used in fur farming to reduce immunodeficieny States, is provided with the growth and shedding puppies. Also, this product is well proven in the preparation of animals for mating and seleniu.

Thus, immunomodulator has a high effect of increasing the General and the specific resistance of the organism.

The present invention in comparison with the prototype and other known technical solution has the following advantages:

the biostimulant obtained by the present invention has the effect of tissue therapy, protein gidrolizatami, organotherapy, nonspecific proteinuria and bactericidal effect;

- cooking method allows not only to obtain the drug with the effect of tissue and other types of therapy, but also allows you to keep useful properties of eggs: high amino acid composition, vitamins and hormones, high immune indicators, activation of metabolism;

cheaper production;

- basic techniques, drugs, equipment used in the preparation of KIM easily accessible.

The method of preparation of an immune modulator, comprising cooling the selected fertilized uninfected eggs at a temperature of 2-4°up to 7 days, homogenization, dilution with saline egg masses, bactericidal treatment, the preservation solution of phenol to his 0,3%concentration, with subsequent filter is a function of the target product, characterized in that the egg mass is diluted with a physiological solution at a ratio of 1:5, and then conducting heat treatment in a water bath at a temperature of 70-80°C for 1 hour before conservation target product is cooled in a germicidal chamber under a lamp with UV irradiation for 30-40 minutes



 

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