Method for reducing artificial electromagnetic radiation action hazard

FIELD: medicine.

SUBSTANCE: method involves forming electromagnetic wave directed towards biological object having predominating right polarization in the electromagnetic oscillation-emitting source system.

EFFECT: reduced risk of harmful action upon biological object objects.

3 tbl

 

The technical field

The invention relates to medicine and medical engineering, electrical engineering, communication technology and can be used to reduce the risk of impact on living organism artificial electromagnetic radiation (EMR), causing significant disturbances in the physiology and functions of the organism, i.e. representing a danger to the health and life of man.

The level of technology

Known methods of protecting the person from artificial AMY and devices for their implementation, based on the creation of sources of electromagnetic fields, compensating fluctuations of the electromagnetic fields of artificial origin.

For example, known is a device for balancing the ambient electromagnetic field, which as a countervailing source of oscillations of the electromagnetic field using several sources of electromagnetic fields, balancing the harmful radiation from the screens of cathode ray tubes (CRT) and vehicles with engines. It can be installed in vehicles, and balancing the electromagnetic field of radiation sources should be not less than three (SU, 1718710, 1992).

The specified device and implemented in the it method does not protect against other types of radiation unnatural origin (TV and radio stations, radar installation is key, cell phones and so on).

The known method and device for protecting humans from artificial AMY by restoring the function of the thymus gland, which consists in human exposure to low frequency electromagnetic field, in which the frequency of oscillation of the electromagnetic field corresponds to the oscillation frequency of the thymus gland, while the current value of the intensity of exposure to govern in accordance with the current value of the voltage between the points of projection of the thymus person (EN, 2138304, 1999).

However, this method and apparatus are characterized by great complexity. Analogues close to the claimed method was not found.

The invention

The aim of the invention is to provide a simple and convenient method of reducing the risk of exposure to the ELECTROMAGNETIC radiation on biological objects.

The technical result achieved by the invention is to reduce the risk of exposure to biological objects of artificial AMY.

The mentioned technical result is achieved by forming affecting living organism AMY, predominant right polarization spatial patterns (PPP).

Biological object (BO) is an open system, receiving electromagnetic waves (EMW), coming from sources of natural and artificial origin, and f is maruosa own EMR. The range of the electric wave that can be perceived BO, covers the entire spectrum of ELECTROMAGNETIC radiation.

It is known that numerous functional and organic changes in the life and development of biological systems due to complex perturbations in the external physico-chemical environment, the source of which are cosmic influences. The main role of the EMP [1, 2].

The disease can be seen as a violation of the spatial-temporal organization of systems and subsystems BO, caused by various disturbances in the external environment, which can be attributed disharmonizing AMY, preventing the normal functioning of the body.

The easiest way to reduce the adverse effects of artificial AMY on BO - formation of spatio-temporal patterns AMY, not introduce disharmony in BO. At the same time or spectral characteristics of the artificial AMY almost impossible to harmonize with the temporal organization of life processes BO, because the spectral characteristics of the artificial AMY are formed on the basis of specific technical tasks.

Reduction not in the picture effect AMY associated with its spatial structure is based on the following.

Discovered by Louis Pasteur disymmetry living organisms due to homochirality to usevich biopolymer structures: nucleic acids contain only L-isomers of sugars, enzymes are built from only the L-isomers of amino acids [3]. Homochirality or chiral purity is a distinctive property of all living things on Earth. The manifestation of rightism-leftism is expressed in a helical (spiral) the distribution of atoms in chemical compounds - right or left.

Vernadsky was first pointed out the stark differences that characterize proviso and leftism in relation to energy phenomena AMY, and their relationship with the manifestation of dissymmetry in living matter [4].

Living matter, characterized by a predominant molecular dosimetria expressed in the spin in the right spiral crystals and molecules essential for life connection of proteins, sugars, etc. that selectively responds to the coherent EMR with different PPS. The simplest spatial structure is an ordered coherent radiation is generated at the linear and elliptical polarizations. Elliptically polarized AMY characterized by a right or left asymmetry, defined by the direction of the twist of the helix formed by the end of the vector of electric field intensity in the propagation of EMW [5]. A linearly polarized electromagnetic wave is characterized by a double asymmetry (left and right), as it can be present as a result of the interaction of waves with right and left circular polarization [6].

Polarization spatial is the first structure propositional EMW to the greatest extent consistent with the prevailing spatial structure of fixed for the life of BO connections.

Below is a list of the most recurrent biological effects of artificial AMY:

- effects on growing cells, for example, the increase in the rate of cancer cell division;

- increased incidence of cancer;

- violation of embryonic development;

- mental abnormalities as a result of changes neurochemistry laboratory;

the increase in other degenerative diseases (such as Parkinson's disease and others) [7].

The devastating effects of artificial ELECTROMAGNETIC radiation can be significantly reduced by forming affecting BO EMW propositional spatial structure.

We obtain a reduction of the damaging effects of artificial AMY on BO explained as follows.

In the formation of chiral purity BO in the process of evolution was part of the global space and geophysical factors.

Asymmetry is observed in a variety of classes cosmic phenomena.

Therefore, the effect of EMW with faculty, meet the corresponding spatial structure of the physical environmental factors related to the evolution BO, makes less disharmony in the functioning of BO.

Materials

In the materials of the results of biological studies to assess the risk of exposure to EMR with different spatial polarization the second structure.

The results of the study confirm that propositione AMY is safer to BO compared with linear and leopoldinum AMY.

Information confirming the possibility of carrying out the invention

The use of elliptically polarized AMY in radio systems and communication systems is well known [5].

They are used in radar, satellite television, communication systems, etc.

Technical implementation of well-designed and simple (for example, a spiral antenna, turnstile antenna with a phase shift of 90%).

One of the proposed options for the use of the invention is a mobile communication system. Currently, the main source of artificial AMY, superior one-two orders of magnitude the level of radiation of microwave ovens and the signal from radio and television stations, are cell phones and mobile communication systems.

The situation is complicated by the fact that cell phone antennas are in close proximity to the human head.

The formation propositional AMY at the output antenna of mobile phones (in contrast to the currently used antenna with linear polarization) will significantly reduce their adverse effects on humans.

The achievement of this result is confirmed by the experiment is antolino. The experiments were carried out on mice CBA and peripheral human blood.

Biological objects were subjected to irradiation of ELECTROMAGNETIC radiation with a frequency of 900 MHz at three different types of polarization spatial patterns (linear, left and propositione AMY).

It is established that propositione AMY's safer for human health compared with linear and leopoldinum AMY.

RISK ASSESSMENT of EXPOSURE to ELECTROMAGNETIC RADIATION WITH DIFFERENT POLARIZATION SPATIAL STRUCTURE ON BIOLOGICAL OBJECTS

Assessing the impact of electromagnetic radiation (EMR) with different spatial polarization structure on biological objects was performed in three models:

1. Evaluation of the cytotoxic effect on lymphocytes in human peripheral blood in vitro.

2. Evaluation of humoral immunity in mice of CBA.

3. Evaluation of phagocytic activity of neutrophils in the peripheral blood in mice CBA immunized with sheep erythrocytes.

Evaluation of the cytotoxic effect of

In the experiment we used peripheral blood lymphocytes of 10 voluntary donors: 6 women and 4 men aged 20-35 years.

Each donor was taken in 3 ml of blood.

The cells in sterile conditions were identified on the gradient fillarray with a density of 1.077. After that lymphocytes twice tivali medium 199.

Cells transferred into the culture medium, consisting of 4 parts of RPMI-1640 medium with glutamine, and 1 part inactivated bovine serum. In the prepared medium was added heparin (concentration in environment 1.5 U/ml), penicillin and gentamicin sulfate.

Lymphocyte culture was divided into 4 equal parts. Three samples were subjected to EMP pulse on laboratory microwave installation within 60 seconds on the 2nd and 3rd day of cultivation. The samples were irradiated in the mode of linearly and elliptically polarized pulsed microwave radiation (left and right polarization), modulated frequency sound range with flux density average power of 5 mW/cm2pulse power flux-density 28 W/cm2, SAR 2 W/kg and the coefficient of ellipticity of the polarization characteristics of the microwave emitter of 0.7. The frequency of the microwave radiation of 900 MHz in the UHF range. The fourth part of the culture of lymphocytes from each donor were subjected to spurious radiation.

Lymphocytes were cultured at 37°within 72 hours.

To assess the viability of the cells lymphocytes were stained with a mixture of dyes Acridine orange (0,008%) and ethidium bromide (0,025%).

Ethidium Bromide stains the nuclei of dead cells in red. Acridine orange stains live metabolizing cells in green color.

For the procedures of the painting lymphocyte culture was centrifuged, then added to the working solution of the dyes.

Lymphocytes were stained for 10 min at a temperature of 36°C. the Paint washed twice with medium 199. The sections were analyzed using a fluorescence microscope LUMAM AND-2. Analyzed at least 500 cells for each mode of exposure. To viable attributed cells with green fluorescence in the cytoplasm. A compromise was attributed cells with green fluorescence in the cytoplasm and the red fluorescence of the nucleus. Dead cells considered in the case of the red glow of the nucleus and the absence of green fluorescence in the cytoplasm.

Evaluation of humoral immunity

Assessment of the impact of ELECTROMAGNETIC radiation with different polarization spatial structure on the humoral immune response was assessed by the content of antibody productive cells (AFC) in the spleen of animals using the method proposed by Jerne, N., Nordin, A. (1963) modified by Lamparski NN. (1969).

Method local hemolysis in a monolayer of red blood cells allows us to quantitatively assess the immunological activity of the spleen cells of animals. This method allows the quantitative study of complex formation of b-lymphocyte antibodies in vivo and in vitro.

In the experiments used male mice of CBA at the age of 70-80 days, weighing 22-24 g

Was formed 4 experimental groups of 10 animals each:

<> 1. Impact on animals linearly polarized AMY.

2. Impact levabyarezhnaya AMY.

3. Impact propositional AMY.

4. Control.

Animals were subjected to EMR within 7 days prior to immunization within 5 days after immunization for 60 seconds on a daily basis. Mice were irradiated in a cylindrical cartridge of glass 2-3 animals in the mode of linearly and elliptically polarized pulsed microwave radiation (left and right polarization), modulated frequency sound range with flux density average power of 5 mW/cm2pulse power flux-density 28 W/cm2, SAR 2 W/kg and the coefficient of ellipticity of the polarization characteristics of the microwave emitter of 0.7. The frequency of the microwave radiation of 900 MHz in the UHF range.

The intensity of the humoral immune response was assessed at 5 days after immunization of mice with sheep erythrocytes (EB) (intraperitoneal injection of 1x108EB).

On the 5th day after immunization, the mice were scored by the method of dislocation of the cervical vertebrae was removed spleen. From a sample of the spleen (40 mg) of the above-described method of preparing a suspension of cells in 4 ml of chilled medium 199 and counted the number of nucleated elements in the camera Goryaeva. A suspension of splenocytes were mixed in equal volumes (0.1 ml) with 10% suspension of DL (this was taken the same EB, which immunis the listed animals) and complement (dilution 1:5) pre-exhausted DL. The obtained mixture was filled in a special chamber made of a subject and cover glasses. The chamber was sealed with vaseline, put in a humid atmosphere, and incubated in a thermostat at a temperature of 37°C for 90 minutes.

During incubation of plasma cells synthesize antibodies, which diffuse in the environment and are fixed on the erythrocytes. In the presence of complement occurs lysis of erythrocytes. Formed zone of hemolysis - large bright spots (sometimes called plaques)that are visible to the naked eye. In the center of each zone is AOK. In General, the number of KLA corresponds to the number of zones of hemolysis. The severity of the immune response was assessed by the number AOK 1 million splenocytes and on the body.

Evaluation of phagocytic activity of neutrophils in the peripheral blood in mice immunized with sheep red blood cells

Assessment of the impact of EMI with different spatial polarization structure on the phagocytic activity of neutrophils in the peripheral blood was assessed by the ability of neutrophils to phagocytose granules latex.

In our experiments we used female mice CBA at the age of 70-80 days, weighing 18-20 g

Was formed 4 experimental groups of 7 animals each:

1. Impact on animals linearly polarized AMY.

2. Impact levabyarezhnaya e is I.

3. Impact propositional AMY.

4. Control.

Animals were subjected to EMR within 7 days prior to immunization within 5 days after immunization for 60 seconds on a daily basis. Mice were irradiated in a cylindrical cartridge of glass 2-3 animals in the mode of linearly and elliptically polarized pulsed microwave radiation (left and right polarization), modulated frequency sound range with flux density average power of 5 mW/cm2pulse power flux-density 28 W/cm2, SAR 2 W/kg and the coefficient of ellipticity of the polarization characteristics of the microwave emitter of 0.7. The frequency of the microwave radiation of 900 MHz in the UHF range.

Phagocytic activity of neutrophils in the peripheral blood was determined in mice immunized with sheep erythrocytes (EB).

Mice were senzibilizirani intraperitoneal introduction of 1x107EB. After 96 hours under the skin of the right rear foot was introduced allowing the dose of antigen in the amount of 1x108DL in 20 μl of sterile saline. 24 hours after the introduction of the resolving dose was determined phagocytic activity of neutrophils.

Blood sampling in mice was performed after decapitate.

Neutrophils were isolated by gradient ficol-urographine with density gradients 1,089 - for macrophages (neutrophils and monocytes) and 1,077 - lymphocytes.

Stoichiometric is silt washed twice with medium 199. Next, 1 ml of cell suspension was placed in a test tube-boots, in which at the bottom put the cover glass. For the adhesion of suspended cells were incubated in a thermostat at 37°within hours.

Thrice washed cells adhered on the glass medium 199. After laundering was added 1 ml of medium 99 and 100 µl of the working solution of latex, shook, was placed in a thermostat at 37°C. After 30 minutes incubation, the slides were washed from excess latex.

The preparations were dried and fixed in formaldehyde vapor for 20 minutes.

Cells were stained with safranin for 10 minutes.

Analyzed the content of granules latex 100 neutrophils. Expected percentage of active cells (with latex granules in the cytoplasm) and the intensity of phagocytosis as the average number of latex particles captured 1 by neutrophil.

RESULTS

Evaluation of the cytotoxic effect of EMI with different polarization spatial structure

Various adverse effects may lead to cell death. One of the most sensitive to the action of adverse factors in human peripheral blood lymphocytes. Numerous studies have shown the increase of interphase (apoptotic) death of lymphocytes, for example, when ionizing effects. In our research we actualizations lymphocytes in human peripheral blood when exposed to cells AMY with different spatial polarization structure.

As a result of the research it was revealed that linearly polarized AMY leads to a significant reduction in the frequency of viable lymphocytes. Thus, if the control frequency of viable cells was 87.8%, linearly polarized AMY led to decline by 12% (table 1). Effects on lymphocytes levabyarezhnaya AMY also resulted in a significant reduction in the frequency of viable cells, but these changes were less pronounced. Propositione AMY, in contrast to linearly polarized and levabyarezhnaya exposure did not change the frequency of viable cells, and it actually match the control level. It should be noted that the frequency of viable cells when exposed prepolarization AMY was significantly different from that of the index in effect on the human lymphocytes linearly polarized AMY.

The revealed changes was determined by the increase in the frequency of cell death by exposure to linearly polarized and levabyarezhnaya AMY (table 1). Frequency compromise cells was significantly longer in both cases, and the proportion of dead cells in the case of exposure to linearly polarized AMY was 1.4 times higher than in the control. In all cases, the impact propositional AMY was distinguished from the others I did experienced the effects. And if the share of the compromise of cells this effect was somewhat larger than in the control, the frequency of dead cells on the contrary was even slightly lower than the control values. When analyzing these parameters, the effect propositional AMY advantageously significantly differed from the effect of linearly polarized ELECTROMAGNETIC radiation.

Thus, the analysis of the obtained data it is possible to come to the following conclusion:

1. Linearly polarized AMY leads to an increase in the frequency of deaths of peripheral blood lymphocytes in vitro.

2. Levabyarezhnaya AMY leads to an increase in the frequency of deaths of peripheral blood lymphocytes in vitro, but this effect is less pronounced compared to the effect of linearly polarized ELECTROMAGNETIC radiation.

3. Propositione AMY does not increase the frequency of death of lymphocytes in human peripheral blood in vitro, and this parameter is significantly different from the action of linearly polarized ELECTROMAGNETIC radiation.

Evaluation of EMI with different polarization spatial structure on the immune system

The immune system is one of the most sensitive to the action of a wide range of adverse factors. We evaluated the influence of EMI with different spatial polarization structure on indicators of humoral immunity (determining the number of AFC in the spleen) and phagocytic activity of neutrophi the s peripheral blood.

As a result of the research it was revealed that linearly polarized and levabyarezhnaya AMY lead to hyperactivate the antibody productions that were manifested in the increase in the concentration of AFC in the spleen (number AOK 106nucleated cells). This effect levabyarezhnaya AMY exceeded the action of linearly polarized radiation. Thus, the concentration of the KLA when exposed levabyarezhnaya AMY exceeded targets approximately 1.7 times, and the linearly polarized AMY 1.6 times (table 2). The absolute number of AFC in the spleen when exposed levabyarezhnaya AMY 40% was higher than in control, and was again led to a more pronounced effect compared with the effect of linearly polarized ELECTROMAGNETIC radiation.

Propositione AMY did not modify the immunological reactivity of animals. And in all cases statistically significantly differed from the effects of both linear and levabyarezhnaya AMY.

In the evaluation of phagocytic immunity have been identified, in General, similar results. And in this case, linearly and levabyarezhnaya AMY led to hyperactively immune reactivity. These impacts caused an increase in the frequency of active neutrophils in 1.6 and 1.5 times, respectively (table 3). Increased phagocytic activity of neutrophils was evident in led is the increase in the number of latex particles per 1 neutrophil. In this case, registered a twofold increase in the case of exposure to linearly polarized AMY. In the evaluation of phagocytic activity of neutrophils effect linearly polarized AMY was more pronounced than when exposed to EMR from the left polarization.

Prepolarization AMY did not lead to changes in the phagocytic activity of neutrophils and significantly differed from the linear and levabyarezhnaya radiation when assessing the frequency of active neutrophils. In addition, significant differences were recorded when comparing the average number of latex particles per 1 neutrophil in group with right - and linearly polarized ELECTROMAGNETIC radiation.

Thus, when analyzing the influence of EMI with different polarization spatial structure of the immunological reactivity can come to the following conclusion:

1. Linear and levabyarezhnaya AMY leads to hyperactively immune reactivity.

2. Propositione EMP does not affect the immune reactivity.

When considering the identified effects from the point of view of danger to human health can be concluded:

1. Propositione AMY is safer for human health compared with linear and leopoldinum AMY.

Table 1
The cytotoxic effect of EMI with different spatial polarization structure in the lymphocytes of peripheral blood in vitro
Experimental impactThe frequency of cells%
Viable cells
Control87,8±1,2
Linearly polarized AMY* 75,7±2,2
Levabyarezhnaya AMY* 80,5±2,6
Propositione AMY+86,1±1,4
Compromise cell
Control7,9±1,1
Linearly polarized AMY* 18,3±2,2
Levabyarezhnaya AMY* 14,5±2,3
Propositione AMY+10,2±1,2
Dead cells
Control4,3±0,3
Linearly polarized AMY* 6,0±0,7
Levabyarezhnaya AMY5,0±1,0
Propositione AMY+3,7±0,6
Note: * - significant difference from control, P≤0,05; + reliable differences from groups with linearly polarized AMY, R≤0,05.
Table 2
The influence of EMI with different spatial polarization structure on the amount of antibody productive of spleen cells from CBA mice
Experimental impactQty AOK 106Askthe absolute quantity of the KLA, ×104
Control749±579,75±0,59
Linearly polarized AMY* 1182±13412,16±1,11
Levabyarezhnaya AMY*1254±110* 13,64±1,03
Propositione AMY+769±62+8,70±0,72
Note: * - significant difference from control, P≤0,05; + reliable differences from groups with linearly polarized AMY, R≤0,05.

Table 3
The influence of EMI with different spatial polarization structure on the phagocytic activity of neutrophils in the peripheral blood of mice immunized with sheep red blood cells
Experimental impactFrequency of active neutrophilsThe average number of latex particles per 1 neutrophil
Control23,8±3,31,02±0,29
Linearly polarized AMY* 38,5±3,5* 2,03±0,36
Levabyarezhnaya AMY* 35,0±2,91,75±0,35
Propositione AMY+22,8±2,9+1,05±0,30
Note: * - significant difference from control, P≤0,05; + reliable differences from groups with linearly polarized AMY, R≤0,05.

Bibliography

1. Chizhevsky Appellee echo of solar storms. M: Idea, 1976

2. Demishkan A.S. Electromagnetic fields and wildlife. M.: Nauka, 1968

3. As he destroyed the mirror symmetry of the biosphere. In the world of science (Scientific American). Ed. in the Russian language. M.: Mir, 1989, No. 10.

4. Vernadsky V.I. ABOUT pravicne and leftism. Problems of biogeochemistry: Proceedings. biogeochemical laboratory. M: NAU is a, 1980, Vol.16.

5. Antenna elliptical polarization. Theory and practice: Sat. articles. M: Foreign literature, 1961

6. Nicholas CENTURIES Electrodynamics and wave propagation. M.: Nauka, 1973

7. Description of the invention to the patent of the Russian Federation RU 2138304 C1, 27.09.99.

The way to reduce the risk of impact on biological objects of artificial electromagnetic radiation, characterized in that the radiating system source of electromagnetic waves is formed in the direction of the biological object, an electromagnetic wave with a dominant right polarization.



 

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