Preparation applied for predicting subclinical mastitis in cattle
FIELD: veterinary science.
SUBSTANCE: the suggested preparation consists of sulfonol and an indicator as neutral red, methylene blue and it additionally contains caustic potash at the following ratio of components, weight%: technical sulfonol 4.75-4.77, neutral red 0.001-0.003, methylene blue 0.001-0.003, caustic potash 0.09-0.11, water - the rest. This preparation enables to predict subclinical mastitis in cattle at earlier stage of disease.
EFFECT: higher accuracy and efficiency of diagnostics.
1 ex, 1 tbl
The invention relates to veterinary medicine and can be used for the diagnosis of subclinical mastitis in cows.
Know the diagnostic tool subclinical mastitis by determination of lysozyme in milk. It is based on the ability of lysozyme milk to retard the growth of the test organism (Staphylococcus aureus strain VM). The zone of growth inhibition of judge titer of lysozyme in the milk and the health of the mammary gland (3).
The use of this diagnostic tool in the conditions of economy practically difficult to implement, since it requires a specially equipped laboratory and certain skills in microbiological research requires culture of the organism.
Known means of determining the amount of chloride in milk as an indicator of the presence of subclinical mastitis. In the milk of a healthy cow contains 0.08 to 0.14% of chlorides. When mastitis their number in the contents of the udder increases. In tubes make 5 ml of 1.5%solution of silver nitrate, 2 drops of a 10%aqueous solution of hromovokislyj potassium and add 1 ml of the investigated milk. The contents of the tubes are mixed. If in case milk, the quantity of chlorides greater than 0.14%of the mixture acquires a yellow color (2).
The disadvantage of this tool is the fact that milk is not always created necessary to determine the concentration is s ions of chlorine.
Know the diagnostic tool subclinical mastitis, based on the determination of chlorine-sugar numbers (the ratio of chloride and lactose). Lactose (milk sugar) is determined by iodometric method (5).
The disadvantage of this tool is that it is a chloro-sugar number can be changed and other diseases, including when ketosis, and thereby lead to distorted results.
Know the diagnostic tool subclinical mastitis by measuring the pH of milk. If the milk from a healthy animal has a pH average of 6.3 or 6.9, under pathological conditions, the reaction medium is shifted to the alkaline side - 7.0 and above. To do this, on a white porcelain plate put 2-4 drops of the investigated milk and add to them 1-2 drops of 0.2%alcohol (65 ° alcohol) solution bromthymol blue. Staining of milk in yellow or greenish-yellow color is taken as an indicator of normal milk; blue-green or green-yellow staining of the sample is a sign of subclinical mastitis (4).
The disadvantage of this diagnostic tool is that the secret of breast cancer has a high buffer capacity and neutralizes hydrogen ions, preventing the display of the changed pH.
Known diagnosis of subclinical mastitis based on the detection of blood pigments in the milk. For e the CSOs in test tube add 5 ml of 3% hydrogen peroxide solution and 2 ml of saturated solution of benzidine in glacial acetic acid. The contents of the tube thoroughly shaken and then dropwise introduced into the test tube and analyzed milk (2-10 drops). The number of drops of milk, made in a test tube, in which a positive reaction, it is possible to judge the amount of blood pigments in the milk. With the positive reaction the contents of the tubes initially acquires a greenish color, but after 5-10 minutes it becomes dark blue. Negative reaction - content light at the bottom of the tube appears whitish flocculent precipitate (1, 4).
The disadvantage of this tool is that in the initial stages of subclinical mastitis reaction to blood pigments are not always a reliable sign of an emerging disease, while in some physiological and pathological conditions of the udder, not associated with mastitis, they will be positive.
Known diagnosis of subclinical mastitis, based on the combined application of surfactants and indicators. Based on this principle is used such a drug as dimaston chosen as a prototype. Diagnosis of these funds is based on the formation of a clot from a somatic cell, the number of which increases in milk during mastitis, and change its color (change of pH and as a consequence changed the e color indicator). In the diagnosis of mastitis 5% solution of dimattina reaction take into account the following indicators: negative reaction (no mastitis) - by mixing 1 ml of a 5%solution of dimattina with 1 ml of milk forms a homogeneous liquid, color - orange, orange-red. Doubtful reaction - traces of jelly formation, color - red. A positive reaction is clearly visible clot that is half or completely ejected from the crescents stick with stirring, color: crimson, crimson, crimson (3).
The advantage of these tools is that they can be used in any sector, not taking the cost of expensive equipment.
The disadvantages of these drugs are that, firstly, their composition does not take into account the high buffering capacity of milk and the indicator light starts to change its color with a significant shift in the reaction environment; secondly, the indicators included in their composition, such as phenol red in dimattina, begins to change its color when pH=7.2 and above, while in subclinical mastitis change in pH, indicating the onset of the disease occurs with 7.0 and above.
The aim of the invention is a means of diagnosis of subclinical mastitis in cows, which allows to detect this disease at an earlier stage.
This objective is achieved in that the development tools is iagnostic subclinical mastitis using features of the udder secretions in this disease contain an increased number of somatic cells and have a pH above 7.0 and. This tool contains (wt.%): the sulfonic acid technical 4,75-4,77, neutral red 0,001-0,003, methylene blue 0,001-0,003, caustic potassium 0,09-0,11 to reduce the buffering capacity of milk.
To indicate an increased number of somatic cells in milk using their property to form a gel-like clot upon contact with the surface-active agent (surfactant). Surfactants, destroying the nucleus of somatic cells, forms complexes with proteins and their displaces DNA from the bound (protein) in the free polymer state in which it is due to the peculiar structure of the macromolecule (elongated) is viscous.
As the surfactant used the sulfonic acid technical. From him prepare for a solution, 100 g of which contains 5 grams of powder. The solution is used after 3-day exposure.
To register a change of pH, we chose the indicator, consisting of equal parts of a 0.1%aqueous solution of neutral red and 0.1%solution of methylene blue. This mixture differs clear color transition when changing pH. In the acidic environment, it has a blue-purple color, and at pH=7.0 and above it goes to green.
The method of preparation of the reagent (reagent is referred to Trinity mastitis test (TMT): the indicator is mixed with solution of sulfonic acid 1:20 add potassium hydroxide 0.1 g per 100 g of solution.
Comparable analysis claimed the defense and well-known diagnostic tools allows you to identify the signs, distinguish the proposed solution from the prototype: synergistic use of a mixture of caustic potash, which allows to reduce the high buffering capacity of milk, and the indicator that registers due to this, the earlier the change in pH value.
Thus, the claimed means of diagnosis of subclinical mastitis in cattle meets the criterion of "novelty."
The authors offer for the diagnosis of subclinical mastitis to use the reagent in the following way: in the hole petty-control plate of each quarter to milk 1 ml of milk last portion (alveolar milk). Then the analyzed milk mixed with 1 ml of the reagent in the well dairy-control plate.
Accounting reactions lead after 4 seconds according to the degree of the resulting jelly (clot) and color change of the indicator.
The reaction is:
negative (no mastitis), if the hole is homogeneous liquid violet;
is doubtful if in the hole marked traces of jelly formation, which under stirring with a glass or wooden wand reaches for it in the form of thin fibers, the color of the mixture - violet;
positive if they form a thick slime that when mixing easily thrown out of a hole with a glass rod, the color of the mixture - violet, or if formed Guta SG shall flow, and the mixture was painted a greenish or bright green color.
for the implementation of the proposed means and method for the diagnosis of subclinical mastitis was held scientific-production experience.
In OJSC "Shelkovskoy" Sysert district of the Sverdlovsk region was prosledovala 100 cows of black-motley breed dimosthenous breakdown, which identified 8 animals with subclinical mastitis, or 8%. Then those same cows were examined for the presence of subclinical mastitis TMT reagent. Research them fully confirmed the results dimosthenous samples. Additionally it was revealed 7 goals. The total number of animals with subclinical mastitis, totaled 15 goals or 15%.
1. The results of studies on subclinical mastitis
|The number of animals, goal||Patients cows dimosthenous breakdown, goal||Patients cows breakdown "TMT", goal||Additionally, patients breakdown "TMT", goal|
Milk from sick cows were brought to the laboratory, where it was divided into E. coli Str. uberis, St. epidermidis - agents of subclinical mastitis.
Thus, for the diagnosis of subclinical mastitis the cattle can apply our proposed tool, allowing to increase the detection sensitivity, to reduce the cost of preventive and curative work to increase productivity.
The drug is a foaming liquid green. Shelf life is 1 year.
The list of references
1. Alexeev, I., Mirolyubov, M.G. Experience of the fight against mastitis in dairy complexes. - Cheboksary Chuvash publishing house, 1984. - 48 S.
2. Balushkin, CD, Medvedev, GP Obstetrics and bioengineering of animal reproduction: a Textbook, 2nd ed., revised and enlarged extra - Mn.: Uraji, 2001. - 869 S.
3. Iwakura, A.I. System of measures to combat mastitis cows. - M:. Rosagropromizdat, 1991. - 240 S.
4. Ilina A.I., Pospelov A.I. Disease of the udder. L.-M., Symptoms, 1961, 152 S.
5. Kartashova, V.M., Iwakura, A.I. Mastitis cows. - M.: Agropromizdat, 1988. - 256 S.
Tool for the diagnosis of subclinical mastitis in cattle, containing the sulfonic acid and the indicator, wherein the quality indicator is used neutral red, methylene blue, and it additionally contains potassium hydroxide in the following ratio, wt.%:
|The sulfonic acid technical||4,75-4,77|
FIELD: medicinal microbiology.
SUBSTANCE: method involves stage-by-stage immunization of rabbits with lipase from Pseudomonas sp. and preparing anti-lipase antibodies that are immobilized on polymeric microspheres. Then in the reaction of volume agglomeration in analyzed hemolytic strains of choleraic vibrios the property to produce lipase in interaction with anti-lipase antibodies immobilized on polymeric carrier is detected and the positive reaction is found confirming atoxigenic property of the analyzed strain being toxigenic - non-hemolytic strains don't show such ability but show the negative response reaction. Method provides reducing time for detection. Invention can be used in laboratory diagnosis of extreme danger infections.
EFFECT: improved method for detection.
4 cl, 1 tbl, 3 ex
FIELD: medicine, immunology.
SUBSTANCE: method involves determination of thiol-disulfide ratio value (TDR) in blood sample after its incubation with a single dose of antigen in the specific foodstuff. Then in 30 min, 1 and 3 h after onset of incubation 2-, 4- and 8-fold dose of antigen is added repeatedly and the value TDR is determined in interval for 30 min, 1, 3 and 24 h of incubation. Then the curve for dependence of the TDR value from incubation time is plotted and the value AUC (areas under curve) for dependence of the TDR value of the incubation time is calculated. Invention provides the development of a method allowing carrying out assay of allergy, nutritional intolerance or usefulness of foodstuff. Invention can be used in assay of effect of foodstuff on the body.
EFFECT: improved assay method.
4 cl, 3 tbl, 2 ex
SUBSTANCE: method involves evaluating patient state using Hamilton scale, considering leukocyte formula of general clinical blood analysis to determine leukocyte and monocyte number and determining whole blood infrared spectroscopy absorption spectrum values in infrared analyzer within 30 s in the bandwidth of 3085-2832 cm-1 and 1543-1425 cm-1, respectively. Mean value of depression intensity being equal to 19.0±2.1 points, mean absorption values being equal to 40.5±3.2% and 43.4±2.9% in the bandwidth of 3085-2832 cm-1 and 1543-1425 cm-1, respectively in combination with immunity characteristics like monocytosis>5% and lymphocytosis>25% leukocyte formula of general clinical blood analysis being observed, therapeutically resistant reactive depression cases are to be diagnosed.
EFFECT: high accuracy of diagnosis.
SUBSTANCE: method involves evaluating patient state using Hamilton scale, considering leukocyte formula of general clinical blood analysis for determining infrared spectroscopy absorption spectrum values within 30 s in the bandwidth of 3085-2832 cm-1 and 1543-1425 cm-1, respectively. Mean value of depression intensity being equal to 21.0±2.8 points, mean absorption values being equal to 32.3±1.8% and 35.0±2.2% in the bandwidth of 3085-2832 cm-1 and 1543-1425 cm-1, respectively and normal lymphocyte and monocyte content being observed, reactive depression is to be diagnosed. Mean absorption values being equal to 40.5±3.2% and 43.4±2.9% in the bandwidth of 3085-2832 cm-1 and 1543-1425 cm-1, respectively in combination with immunity characteristics like monocytosis>5% and lymphocytosis>25% leukocyte formula of general clinical blood analysis being observed, therapeutically resistant reactive depression cases are to be diagnosed.
EFFECT: high accuracy of diagnosis in differentiating cases showing positive changes as response to pharmacotherapy from therapeutically resistant cases.
FIELD: analytical methods in biology.
SUBSTANCE: invention relates to chemical analysis methods for biological materials and, in particular, concerns a method for determining acetylcholine in biological specimens consisting in incubation of a specimen, centrifugation, take-off of supernatant, preparation of alkaline hydroxylamine reagent by adding equal volume of 3.5 n NaOH to hydroxylamine chloride solution, mixing of thus prepared reagent with test specimen, and addition of iron chloride solution followed by measurement of optical density. Invention is characterized by that, before addition of 3.5 n NaOH, hydroxylamine chloride solution is mixed with 10-14% solution of Na2CO3, incubation of specimen is performed after the latter is mixed with alkaline hydroxylamine reagent at 1:2 ratio for 90-120 min at 33-37°C, centrifugation is preceded by adding aqueous hydrochloric acid solution, and 0.74 M iron chloride solution is added to taken off supernatant.
EFFECT: increased determination accuracy.
2 tbl, 3 ex
FIELD: veterinary medicine, biochemistry.
SUBSTANCE: the method deals with treating the smears with buffer mixture followed by washing with distilled water, drying, additional dyeing with 0.5%-methylene blue followed by washing, drying, detecting enzymatic activity by the quantity of dyed granules. The innovation enables to widen technological possibilities in the field of immunological investigations.
EFFECT: higher accuracy of detection.
5 dwg, 1 ex, 1 tbl
FIELD: veterinary medicine, biochemistry.
SUBSTANCE: the method deals with treating the smears with buffer mixture followed by incubation in darkness for 2 h, washing with distilled water, drying, additional dyeing with 0.5%-methylene blue followed by washing, drying, detecting enzymatic activity by the quantity of dyed granules. The innovation enables to widen technological possibilities in the field of immunological investigations.
EFFECT: higher accuracy of detection.
5 dwg, 1 ex, 1 tbl
FIELD: veterinary, biochemistry.
SUBSTANCE: claimed method includes smear preparation, fixing thereof, washing, embedding and incubation in medium containing buffer solution, distillated water, naphthylophosphate and neutral fast blue dye, followed by washing, addition dyeing and estimation of qualitative coloration.
EFFECT: enhanced processing capability; decreased efforts.
5 dwg, 1 tbl, 2 ex
SUBSTANCE: method involves determining averaged MMPI-pattern, vegetative regulation tendency, central hemodynamics type and prostaglandin concentration in blood serum. The averaged MMPI-pattern being increased, sympathetic vegetative regulation tendency being increased, blood circulation being of hypokinetic type and prostacyclin content being low with thromboxane content being high, EPH-gestosis is to be predicted.
EFFECT: high accuracy of early-stage prognosis.
3 dwg, 4 tbl
SUBSTANCE: the present innovation deals with treating cerebral diseases, Parkinson's disease being among them. One should measure biochemical and immunological blood values of a patient, the values of MAO-B thrombocytes activity, the values of erythrocytes activity, Cu/Zn - TFD, the level of neuromediators of glutamate and taurine in blood plasma and circulating immune complexes (CIC) in blood serum. To perform the impact with laser irradiation it is necessary to choose the patients with all, several or only one value in ranges given below: MAO-B 90-250 nM/mg or MAO-B 0-47 nM/mg, at the norm of 73+7 nM/mg, Cu/Zn - TFD 1.2-2.5 U/mg or Cu/Zn - TFD 0-0.8 U/mg, at the norm of 1.5 ± 0.05 U/mg, CIC low-molecular fractions 100-300 c.u., at the norm of 80 ± 5.0 c.u., CIC average-molecular fractions 48-150 c.u., at the norm of 39 ± 2 c.u., affected ratio of glutamate to taurine. Impact should be carried out intravenously with the help of a light guide at the power at its end being 1 mW and wave length of 632.8 daily for 5 d, every seance lasts for 20 min, then medicinal therapy should be fulfilled without laser impact by repeating the course of laser irradiation in a year. The method enables to increase the quality in treating Parkinson's disease due to stimulating the system of antiradical tissue protection, increased protective body reserves and correction of immune disorders.
EFFECT: higher efficiency of therapy.
FIELD: medicine, gastroenterology.
SUBSTANCE: in case of increased heart rate frequency against initial level at swallowing moment by 16% and less one should diagnose gastroesophageal reflux disease. The innovation being a quick-test diagnostic technique enables to indirectly detect gastroesophageal reflux disease.
EFFECT: higher accuracy and efficiency of diagnostics.
2 dwg, 1 ex, 2 tbl
SUBSTANCE: method involves calculating frostbite severity index (FSI) in conditional units so that any finger volume is assumed to be equal to 1 unit. Injury expanded to middle 1/3 of metacarpal bone in hand and metatarsal bones in foot is assumed to be equivalent to 10 units. 40 units large value is assigned to the case of the whole hand and a half of foot being injured. A victim being admitted in adynamic general cooling state, the calculated index value is incremented by 15 units. Stuporous state being the case, 30 units are added. Convulsive state being the case, 45 units are added. Optimum volume of infusion therapy is calculated from formula V= (FSI x M x h):3+1000,0, where M is the patient weight in kg; h is the patient height in m.
EFFECT: high accuracy of method; improved blood circulation conditions in injured; reduced endogenous intoxication in the organism.
FIELD: medicine, infectology.
SUBSTANCE: the suggested innovation deals with analyzing anamnestic, clinical and laboratory data to detect the values of diagnostic criteria for prognostically valuable signs in points according to special scale followed by calculating the sum of points to predict the result of disease by the value of this sum. Thus, the innovation enables to predict the result of disease in 1 mo of inspection already, and in 6 mo it demonstrates coincidence of prediction in 95% patients. This method could be applied at in-patient stage of therapy and during out-patient stage, as well.
EFFECT: higher accuracy and efficiency of prediction.
2 cl, 4 ex, 6 tbl
FIELD: medicine, therapy, rheumatology.
SUBSTANCE: one should detect mineral density of bony tissue in lumbar vertebral department, in column femoris; from patient's life anamnesis one should detect risk factors for osseous fractures: duration of menopause, duration and index of disease severity degree, systemic manifestations of rheumatoid arthritis (RA), solitude, dosage and duration for the intake of glucocortico-steroid (GCS) preparations to establish their gradations and digital values: no risk factor - the sign has got digital value "0", if risk factor is available - "1". Then it is necessary to detect prognostic coefficients F1 and F2 by the following formulas: F1 = -38.72 - 7.27*X1 + 9.77*X2 + 6.30*X3 +4.25*X4 + 7.41*X5 + 9.66*X6 + 32.97*X7 + 3.62*X8 + 8.96*X9 + 38.04*X10 - 0.55*X11 - 0.50*X12; F2 = -5.41 - 4.32*X1 + 3.22*X2 + 2.91*X3 + 3.28*X4 + 3.24*X5 +3.93*X6 + 7.43*X7 +1.35*X8 + 1.08*X9 + 9.07*x10 - 0.11*X11 - 0.19*X12, where X1-12 - gradations and digital values of risk factors (X1 - age is above 45, X2 - postmenopause is above 5 years, X3 - RA duration is above 10 years, X4 - severity degree index is above 5 points, X5 - RA systemic manifestations, X6 - solitude, X7 - intake of GCS preparations is above 2 years, X8 - cumulative dosage of GCS is above 5000 mg, X9 - mineral density of bony tissue in lumbar vertebral department by T-criterion is below -2.5, X10 - mineral density of bony tissue in collum femoris by T-criterion is below -2.5, X11 - mineral density of bony tissue in collum femoris is below 0.700 g/sq. cm, X12 - mineral density of bony tissue in lumbar vertebral department is below 0.900 g/sq. cm). At F1≥F2 one should predict high risk for fracture possibility, and at F1<F2 - low risk for fracture chance. The innovation enables to take into consideration the sum of common and specific risk factors.
EFFECT: higher accuracy of prediction.
4 ex, 1 tbl
FIELD: medicine, functional diagnostics, clinical geriatrics.
SUBSTANCE: one should register functional values of different systems: cardio-vascular, respiratory, autonomic and muscular, calculate the deviation of absolute value in every tested parameter (X1) against normative value (X0), values for each concrete parameter should be taken out of table 1. Then one should calculate the deviation of function value against standardized value (%) by taking into consideration that X0 being equal to 3δ corresponds to 100%-measurement of the parameter, then one should calculate the value for the stability of systemic organization of functions (VSSOF) which is equal to the sum of deviation squares of all relative values of tested parameters referred to the number of tested parameters. If VSSOP is below 920 one should detect relative norm, at VSSOF ranged 920-1380 - I degree of polyfunctional insufficiency manifestation, and at VSSOF ranged 1380-1840 - II degree, and VSSOF above 1840 - III degree. The method enables to improve evaluation of complex functional disorders based upon detection of functional state in senile patients with polymorbidity at conducting medico-social examination and to control the efficiency of restorative therapy.
EFFECT: decreased functional disorders.
SUBSTANCE: method involves carrying out infrared saliva spectroscopy and determining mean values of infrared radiation pass band in frequency ranges of 3085-2832 cm-1, 2120-1880 cm-1, 1600-1535 cm-1, 1543-1425 cm-1, 1430-1210 cm-1, 1127-1057 cm-1. Mean value in the range of 1600-1535 cm-1 being greater than 35.9, in the range of 1543-1425 cm-1 being greater than 57.9, in the range of 1430-1210 cm-1 being greater than 54.5, compensated chronic tonsillitis is considered to be the case. The mean value in the range of 1600-1535 cm-1 being less than 32.5, in the range of 1543-1425 cm-1 being less than 65.2, compensated chronic tonsillitis is to be diagnosed. The mean value in the range of 1600-1535 cm-1 being less than 35.9, in the range of 3085-2832 cm-1 being greater than 72.8, compensated chronic tonsillitis is to be diagnosed. The mean value in the range of 1600-1535 cm-1 being less than 35.9, in the range of 1127-1057 cm-1 being greater than 51.7, compensated chronic tonsillitis is to be diagnosed. The mean value in the range of 1600-1535 cm-1 being less than 35.9, in the range of 2120-1880 cm-1 being greater than 79.7, compensated chronic tonsillitis is to be diagnosed. The mean value in the range of 1600-1535 cm-1 being less than 35.9, in the range of 2120-1880 cm-1 being less than 79.7, decompensated chronic tonsillitis is to be diagnosed.
EFFECT: high reliability and simplicity of differential diagnosis.
SUBSTANCE: method involves fills questionnaire in early postoperative period according to scheme 1, doctor builds a map for estimating life quality of surgical patient in early postoperative period according to scheme 2 using the obtained data. Next to it, clinically significant changes are determined between control points on the map according to scheme 2. The changes being 5-10 points heavy, weakly expressed differences are found to be the case. The value being 10-20 points heavy, the differences are found to be of moderate severity degree. The value being greater than 20 points, the differences are found to be of marked severity in the early postoperative period.
EFFECT: high accuracy in determining general life quality dynamics.
FIELD: veterinary medicine.
SUBSTANCE: method involves separating oviduct from uterus. The oviduct is plunged into reservoir filled with liquid. Air is introduced with overpressure through oviduct infundibulum. Oviduct obstruction is estimated from air discharge rate from the uterine end and pressure drop degree.
EFFECT: high accuracy of diagnosis; simplified instruments used.
1 dwg, 1 tbl
FIELD: medicine; medical engineering.
SUBSTANCE: method involves creating electrostatic field around human body. Field intensity is to provide luminescence of cotton fabric in front of biologically active points having increased electric conductivity. Luminous sites positions are fixed relative to human body outline in dark chamber on the background of illuminated screen. The positions are compared to biologically active point pattern maps. The luminous cotton fabric areas are filmed with digital photographic apparatus or video camera. The luminous cotton fabric areas positions comparison to biologically active point pattern maps is carried out by fitting map scales and superimposing the images. Biologically active points of increased electric conductivity are determined from four sides. Device has dark chamber, electrodes, constant voltage source, unit for moving positive electrode, unit for fixing luminous cotton fabric areas and highlightable background screen. The screen is positioned at a distance from the positive electrode to have enough space for placing human body.
EFFECT: high speed in determining meridian states of biologically active points on human body.
11 cl, 1 dwg
SUBSTANCE: method involves applying chromato-mass-spetrometric techniques for determining small and large intestine bioptate fatty acids content. The number of microbial cells is determined from chromato-mass-spetrogram peak areas. The calculated proportions are interpreted for diagnosing irritated intestine syndrome.
EFFECT: high accuracy of diagnosis.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl