Preparation applied for predicting subclinical mastitis in cattle

FIELD: veterinary science.

SUBSTANCE: the suggested preparation consists of sulfonol and an indicator as neutral red, methylene blue and it additionally contains caustic potash at the following ratio of components, weight%: technical sulfonol 4.75-4.77, neutral red 0.001-0.003, methylene blue 0.001-0.003, caustic potash 0.09-0.11, water - the rest. This preparation enables to predict subclinical mastitis in cattle at earlier stage of disease.

EFFECT: higher accuracy and efficiency of diagnostics.

1 ex, 1 tbl

 

The invention relates to veterinary medicine and can be used for the diagnosis of subclinical mastitis in cows.

Know the diagnostic tool subclinical mastitis by determination of lysozyme in milk. It is based on the ability of lysozyme milk to retard the growth of the test organism (Staphylococcus aureus strain VM). The zone of growth inhibition of judge titer of lysozyme in the milk and the health of the mammary gland (3).

The use of this diagnostic tool in the conditions of economy practically difficult to implement, since it requires a specially equipped laboratory and certain skills in microbiological research requires culture of the organism.

Known means of determining the amount of chloride in milk as an indicator of the presence of subclinical mastitis. In the milk of a healthy cow contains 0.08 to 0.14% of chlorides. When mastitis their number in the contents of the udder increases. In tubes make 5 ml of 1.5%solution of silver nitrate, 2 drops of a 10%aqueous solution of hromovokislyj potassium and add 1 ml of the investigated milk. The contents of the tubes are mixed. If in case milk, the quantity of chlorides greater than 0.14%of the mixture acquires a yellow color (2).

The disadvantage of this tool is the fact that milk is not always created necessary to determine the concentration is s ions of chlorine.

Know the diagnostic tool subclinical mastitis, based on the determination of chlorine-sugar numbers (the ratio of chloride and lactose). Lactose (milk sugar) is determined by iodometric method (5).

The disadvantage of this tool is that it is a chloro-sugar number can be changed and other diseases, including when ketosis, and thereby lead to distorted results.

Know the diagnostic tool subclinical mastitis by measuring the pH of milk. If the milk from a healthy animal has a pH average of 6.3 or 6.9, under pathological conditions, the reaction medium is shifted to the alkaline side - 7.0 and above. To do this, on a white porcelain plate put 2-4 drops of the investigated milk and add to them 1-2 drops of 0.2%alcohol (65 alcohol) solution bromthymol blue. Staining of milk in yellow or greenish-yellow color is taken as an indicator of normal milk; blue-green or green-yellow staining of the sample is a sign of subclinical mastitis (4).

The disadvantage of this diagnostic tool is that the secret of breast cancer has a high buffer capacity and neutralizes hydrogen ions, preventing the display of the changed pH.

Known diagnosis of subclinical mastitis based on the detection of blood pigments in the milk. For e the CSOs in test tube add 5 ml of 3% hydrogen peroxide solution and 2 ml of saturated solution of benzidine in glacial acetic acid. The contents of the tube thoroughly shaken and then dropwise introduced into the test tube and analyzed milk (2-10 drops). The number of drops of milk, made in a test tube, in which a positive reaction, it is possible to judge the amount of blood pigments in the milk. With the positive reaction the contents of the tubes initially acquires a greenish color, but after 5-10 minutes it becomes dark blue. Negative reaction - content light at the bottom of the tube appears whitish flocculent precipitate (1, 4).

The disadvantage of this tool is that in the initial stages of subclinical mastitis reaction to blood pigments are not always a reliable sign of an emerging disease, while in some physiological and pathological conditions of the udder, not associated with mastitis, they will be positive.

Known diagnosis of subclinical mastitis, based on the combined application of surfactants and indicators. Based on this principle is used such a drug as dimaston chosen as a prototype. Diagnosis of these funds is based on the formation of a clot from a somatic cell, the number of which increases in milk during mastitis, and change its color (change of pH and as a consequence changed the e color indicator). In the diagnosis of mastitis 5% solution of dimattina reaction take into account the following indicators: negative reaction (no mastitis) - by mixing 1 ml of a 5%solution of dimattina with 1 ml of milk forms a homogeneous liquid, color - orange, orange-red. Doubtful reaction - traces of jelly formation, color - red. A positive reaction is clearly visible clot that is half or completely ejected from the crescents stick with stirring, color: crimson, crimson, crimson (3).

The advantage of these tools is that they can be used in any sector, not taking the cost of expensive equipment.

The disadvantages of these drugs are that, firstly, their composition does not take into account the high buffering capacity of milk and the indicator light starts to change its color with a significant shift in the reaction environment; secondly, the indicators included in their composition, such as phenol red in dimattina, begins to change its color when pH=7.2 and above, while in subclinical mastitis change in pH, indicating the onset of the disease occurs with 7.0 and above.

The aim of the invention is a means of diagnosis of subclinical mastitis in cows, which allows to detect this disease at an earlier stage.

This objective is achieved in that the development tools is iagnostic subclinical mastitis using features of the udder secretions in this disease contain an increased number of somatic cells and have a pH above 7.0 and. This tool contains (wt.%): the sulfonic acid technical 4,75-4,77, neutral red 0,001-0,003, methylene blue 0,001-0,003, caustic potassium 0,09-0,11 to reduce the buffering capacity of milk.

To indicate an increased number of somatic cells in milk using their property to form a gel-like clot upon contact with the surface-active agent (surfactant). Surfactants, destroying the nucleus of somatic cells, forms complexes with proteins and their displaces DNA from the bound (protein) in the free polymer state in which it is due to the peculiar structure of the macromolecule (elongated) is viscous.

As the surfactant used the sulfonic acid technical. From him prepare for a solution, 100 g of which contains 5 grams of powder. The solution is used after 3-day exposure.

To register a change of pH, we chose the indicator, consisting of equal parts of a 0.1%aqueous solution of neutral red and 0.1%solution of methylene blue. This mixture differs clear color transition when changing pH. In the acidic environment, it has a blue-purple color, and at pH=7.0 and above it goes to green.

The method of preparation of the reagent (reagent is referred to Trinity mastitis test (TMT): the indicator is mixed with solution of sulfonic acid 1:20 add potassium hydroxide 0.1 g per 100 g of solution.

Comparable analysis claimed the defense and well-known diagnostic tools allows you to identify the signs, distinguish the proposed solution from the prototype: synergistic use of a mixture of caustic potash, which allows to reduce the high buffering capacity of milk, and the indicator that registers due to this, the earlier the change in pH value.

Thus, the claimed means of diagnosis of subclinical mastitis in cattle meets the criterion of "novelty."

The authors offer for the diagnosis of subclinical mastitis to use the reagent in the following way: in the hole petty-control plate of each quarter to milk 1 ml of milk last portion (alveolar milk). Then the analyzed milk mixed with 1 ml of the reagent in the well dairy-control plate.

Accounting reactions lead after 4 seconds according to the degree of the resulting jelly (clot) and color change of the indicator.

The reaction is:

negative (no mastitis), if the hole is homogeneous liquid violet;

is doubtful if in the hole marked traces of jelly formation, which under stirring with a glass or wooden wand reaches for it in the form of thin fibers, the color of the mixture - violet;

positive if they form a thick slime that when mixing easily thrown out of a hole with a glass rod, the color of the mixture - violet, or if formed Guta SG shall flow, and the mixture was painted a greenish or bright green color.

Example:

for the implementation of the proposed means and method for the diagnosis of subclinical mastitis was held scientific-production experience.

In OJSC "Shelkovskoy" Sysert district of the Sverdlovsk region was prosledovala 100 cows of black-motley breed dimosthenous breakdown, which identified 8 animals with subclinical mastitis, or 8%. Then those same cows were examined for the presence of subclinical mastitis TMT reagent. Research them fully confirmed the results dimosthenous samples. Additionally it was revealed 7 goals. The total number of animals with subclinical mastitis, totaled 15 goals or 15%.

1. The results of studies on subclinical mastitis

The number of animals, goalPatients cows dimosthenous breakdown, goalPatients cows breakdown "TMT", goalAdditionally, patients breakdown "TMT", goal
1008157

Milk from sick cows were brought to the laboratory, where it was divided into E. coli Str. uberis, St. epidermidis - agents of subclinical mastitis.

Thus, for the diagnosis of subclinical mastitis the cattle can apply our proposed tool, allowing to increase the detection sensitivity, to reduce the cost of preventive and curative work to increase productivity.

The drug is a foaming liquid green. Shelf life is 1 year.

The list of references

1. Alexeev, I., Mirolyubov, M.G. Experience of the fight against mastitis in dairy complexes. - Cheboksary Chuvash publishing house, 1984. - 48 S.

2. Balushkin, CD, Medvedev, GP Obstetrics and bioengineering of animal reproduction: a Textbook, 2nd ed., revised and enlarged extra - Mn.: Uraji, 2001. - 869 S.

3. Iwakura, A.I. System of measures to combat mastitis cows. - M:. Rosagropromizdat, 1991. - 240 S.

4. Ilina A.I., Pospelov A.I. Disease of the udder. L.-M., Symptoms, 1961, 152 S.

5. Kartashova, V.M., Iwakura, A.I. Mastitis cows. - M.: Agropromizdat, 1988. - 256 S.

Tool for the diagnosis of subclinical mastitis in cattle, containing the sulfonic acid and the indicator, wherein the quality indicator is used neutral red, methylene blue, and it additionally contains potassium hydroxide in the following ratio, wt.%:

The sulfonic acid technical4,75-4,77
Neutral red0,001-0,003
Methylene blue0,01-0,003
Caustic potash0,09-0,11
WaterRest



 

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