Method for aseptic obtaining the sperm in farm animals

FIELD: animal science.

SUBSTANCE: the present innovation deals with sanitary-hygienic preparation of exercising area, equipment and animals before sampling the sperm in breeding animals. One should clean the floor in exercising area with catholyte at pH being not less than 11.0 and redox potential - not less than -600 mV; air and floor in exercising area, special equipment for animals as service ramps, artificial vaginas and sperm-collecting reservoirs should be disinfected with anolyte at pH being not more than 2.5, redox potential - not less than 1100 mV and active chlorine content of not less than 0.03%; one should wash animals skin, scrotum and prepuce with anolyte at pH being 7.0±0.5 and redox potential of not less than 900 mV. Moreover, one should treat the air in exercising area with anolytic aerosol at liquid drops diameter being not more than 50 mcm at exposure of not less than 30 min, as for artificial vaginas and sperm-collecting reservoirs they should be kept in anolyte for 30-60 min. The innovation provides to obtain high-quality sperm in farm animals.

EFFECT: higher quality of disinfection.

1 ex

 

The invention relates to the field of animal husbandry, specifically to artificial insemination of farm animals.

The known method aseptic obtain semen of farm animals at the stations of artificial insemination of animals, which includes the complex of preventive measures aimed at preparing the arena premises, equipment used in the capture of the sperm, and the animals themselves before taking sperm and designed to ensure that then high-quality, not contaminated by pathogenic microorganisms sperm and prevention of infectious animal diseases - prototype [1].

In the known method the location of the arena, where they take the sperm, moisturize spray subdesignation solutions, for example furatsilina. Before you begin the air in the arena sterilized bactericidal lamps. If the space station infected spore-forming and UV-resistant microbes, mold, they are processed in pairs formalin. Equipment used in the capture of sperm from manufacturers (machines for Zadok and others), cleaned and disinfected with a solution of caustic soda. Artificial vaginas are sterilized by autoclaving or boiling in distilled water or, as an exception, handle 96°th alcohol; spermaparty article is realizou together with vaginas. The skin, the scrotum and prepuce animals washed with water and wipe dry.

A method of obtaining a sperm has a number of significant drawbacks. First, it is the heterogeneity of the used disinfectants, which complicates efforts to capture sperm. The use of caustic soda, furatsilina, formalin and other chemicals do not give the necessary disinfectant effect, and, in addition, they are expensive and unsafe to use. Bacterial efficiency of UV lamps is not high enough, and they have no memory, because UV light has a bactericidal effect on microorganisms only during operation of the lamps. Moreover, the range of small lamps, and their effectiveness decreases dramatically with distance. Sterilization artificial Vagin and spermogrammu process is energy-intensive, difficult to use, requiring special equipment.

The technical result of the invention is to improve the effectiveness of sanitary measures for obtaining high-quality sperm, the unification of the applicable funds, excluding chemicals and reducing the cost.

This is achieved by a complex of preventive measures sanitary and hygienic nature in preparation for taking the sperm from the producers of the implementation is given with the use of electrochemically activated water. The floors of the arena wash Catolica with a pH of at least 11.0 in units and oxidation-reduction potential (ORP) of not less than -600 mV. Disinfection of floors and air arena, machines for Zadok animals, artificial vaginas and spermogrammu spend acidic anolyte with a pH of no more than 2.5 units, the redox potential of not less than 1100 mV and content of active chlorine of not less than 0.03%. The skin, the scrotum and prepuce animals washed neutral anolyte with a pH of 7.0±0.5 units and ORP at least 900 mV. While the air of the arena is treated with an aerosol of the anolyte with the diameter of the liquid droplets is not more than 50 μm when the exposure is not less than 30 minutes Disinfection artificial Vagin and spermogrammu is carried out by keeping the anolyte within 30-60 minutes

The quality parameters of Catolica (pH and ORP) selected with regard to its detergent properties, which decreases when the parameters below are considerably worse, which does not allow to produce high-quality washing the floors of the arena. Raising options are valid, especially given the different degree of contamination of the sexes in practical terms. So is limited to only the lower limit of quality indicators Catolica.

Accepted as anolyte to disinfect floors and air in the arena and equipment is optimal at pH more than 2.5, the redox potential of less than 1100 mV and the content of active chlorine less than 0.03% of the bactericidal effect of the indoor pool is severely reduced, not providing a disinfecting effect, and at the opposite values bactericidal at high energy is small, and therefore not economically feasible.

PH values and, accordingly, the redox potential of the anolyte for disinfection manufacturers selected from the conditions to prevent can be unpleasant sensations (burning) of animals in the case of acidic anolyte. AFP more than 900 mV does not impair the properties of the anolyte, and the less this value is significantly reduced its disinfecting effect, which is undesirable.

Treatment of air in the arena in aerosol form and the specified dispersion allows readjustment within a relatively short time (30 min). While this time is sufficient for the destruction of sanitary-indicative microflora (bacteria, coliform Bacillus, Staphylococcus and others).

Time-keeping artificial Vagin and spermogrammu in the anolyte set based on the capabilities of disinfection and sterilization) glass, metal, plastic and rubber products. For time less than 30 min, it is impossible to achieve this result, and to withstand more than 60 min is not necessary, because this is enough time for disinfection and sterilisation products from any material.

The method is as follows. Prior work in the area of Manet is a, where do they take the sperm, produce cleaning of floors from mechanical impurities, wash floors with tap water, then jet Catolica a given quality with the help of special equipment (demashin CHATTER, ADP and others) and wash off with a water hose. Then disinfect floors coarse-Sol or jet of acidic anolyte necessary qualities and conduct sanitation air superfine aerosol anolyte for at least 30 minutes at the same time wash the anolyte machines for Zadok animals. The anolyte of the same quality (sour) disinfect the artificial vagina and spermaparty processing within 30-60 minutes sanitization animals spend payom neutral anolyte. Electroactivated water is prepared using a flow diaphragm electrochemical activator, such as type STEL.

An example of using the method. For insemination in the Volgograd region were carried out sanitary-and-hygienic measures in the selection of sperm from bulls of the proposed method. The experiment lasted for 2.5 months. Evaluation of the results of experiments made in comparison with existing farming techniques in the implementation of preventive measures according to the Instructions of the Ministry of agriculture of the USSR [1] - prototype. Activated water (catholyte and anolyte) prigotovlu the Ali apparatus STEL-4H-60.

The experiments showed that the application of electrochemically activated water at the station has increased the quality of disinfection cleaning of floors and equipment, rehabilitation of the air and animals: almost never had to re-processing, as is the case under existing conditions. The costs of preventive measures decreased by 75-80%.

Thus, the proposed method:

- to improve the quality of disinfection, which ensures the production of quality sperm;

- eliminate the cost of purchasing and shipping of chemicals, their preparation in the economy;

- significantly (several times) to reduce the cost of carrying out sanitary-hygienic measures on reorganization;

- to improve the working conditions of personnel and to increase productivity.

Sources of information

1. Instructions on organization and technology work stations and enterprises artificial insemination of farm animals. The Ministry of agriculture of the USSR. - M.: Kolos, 1981. - P.24-30.

1. The way aseptic obtain semen of farm animals, including a range of preventive measures sanitary character of the building of the arena, equipment and animals and taking sperm from manufacturers, characterized in that the floor is of Ania wash Catolica with a pH of at least 11.0 in units and oxidation-reduction potential of not less than -600 mV, the air and the floors in the room of the arena, machines for Zadok animals, artificial vaginas and spermaparty disinfect the anolyte with a pH of no more than 2.5 units, the redox potential of 1100 mV and content of active chlorine of not less than 0.03%, and washing the skin, scrotum and prepuce animals spend the anolyte with a pH of 7.0±0.5 units and oxidation-reduction potential of not less than 900 MB.

2. The method according to claim 1, characterized in that the air of the arena is treated with an aerosol of the anolyte with the diameter of the liquid droplets is not more than 50 μm when the exposure is not less than 30 minutes

3. The method according to claim 1, characterized in that the disinfection of artificial Vagin and spermogrammu is carried out by keeping the anolyte within 30-60 minutes



 

Same patents:

FIELD: equipment for purifying of air drawn into house by oxidizing hazardous chemical substances therein at high temperature with the use of catalyst.

SUBSTANCE: apparatus has cylinder made from refractory electrically isolating ceramics and provided with nichrome incandescent filaments of high resistivity attached to cylinder, fan with electronic control circuit equipped with thermal sensor, switch for switching-on and switching-off of fan engine or regulator and safety device. Apparatus is further provided with housing having cover equipped with detachable filter-sieve and perforated cooling plate. Incandescent filaments are arranged in drum cavity in single plane in equally spaced relation with one another and attached to cylinder through channels in its wall defining grids successively arranged transverse to its axis. Grids are connected in series with supply mains for heating air in grid spacings to temperature of at least 750°C. Cylinder is located in sleeve manufactured from refractory dielectric of low heat-conductivity. Window is provided in sleeve bottom and has diameter equal to cylinder inner diameter.

EFFECT: improved air medium in house owing to increased quality of air purified from hazardous chemical substances contained therein, in particular, from carbon dioxide, nitrogen dioxide, and sulfur dioxide, and reduced consumption of power.

9 cl, 19 dwg

The invention relates to the field of electrical devices used for sterilization of liquid and gaseous media

The invention relates to medical equipment, namely concerns the removal of suspended particles from the air and destroying microorganisms

FIELD: medical engineering.

SUBSTANCE: method involves the following stages: a) applying 0.5-100% aqueous solution of enzymeless cleaning composition concentrate having at least 10% of at least one alkanolamine; b) treating with solution at room temperature to boiling temperature of the solution; c) rinsing. The cleaning composition concentrate is to be applied at stage a) as aqueous solution of 0.5-20% strength, preferentially of 0.5-10% strength, better of 1-5% strength. Treatment at b) stage is carried out at 55° C, preferentially at 35-50° C, better at 40 to 50° C with treatment time being equal to 2-30 min at stage b), preferentially 10 s to 10 min long, better 30 s to 5 min long. The method optionally includes thermal disinfection.

EFFECT: high quality of cleaning; preserved surface safety.

15 cl, 1 tbl

FIELD: medicine, in particular, medicinal materials used for disinfecting treatment of hands, surfaces, injection area or skin portion in the area adjoining to donor's elbow flexure part.

SUBSTANCE: disinfecting diaper is manufactured from absorbing material impregnated with liquid disinfecting composition and has sizes of (135-145) mm x (165-175) mm. Diaper is made from material composed of viscose, polyester or mixture of viscose and polyester. Thickness of material from which diaper is manufactured is 23-25 g/m2 and its absorbing capacity is 9.5-10.0 g/g. Diaper is impregnated with the use of 4.0-5.0 ml of liquid disinfecting composition including alkyl dimethyl benzyl ammonium chloride, alkyl dimethyl(ethylbenzyl) ammonium chloride, glycerol, ethyl alcohol, and water, with components being used in predetermined ratio. According to another version, diaper sized (55-65) mm x (71-81) mm is made of material having thickness of 55-65 g/m2; its absorbing capacity is 10.5-11.5 g/g. Said diaper is impregnated with the use of 1.0-2.0 ml of liquid disinfecting composition.

EFFECT: reduced contamination of environment with treated material, reduced production costs, simplified manufacture process and increased efficiency.

7 cl, 6 ex

Disinfectant // 2252035

FIELD: veterinary science, veterinary disinfection.

SUBSTANCE: for disinfecting the objects of veterinary inspection one should apply a disinfectant that contains 30%-aqueous solution of acetic acid, alkyldimethylbenzylammonium chloride, sodium carbonate, boric acid and trisodium phosphate at a certain ratio of components. The suggested preparation is of wide range of action towards bacteria, viruses and fungi and at concentration of 0.3-1.0% at exposure of 3 h causes 100% lethality of Escherichia coli, Streptococci and Staphylococci.

EFFECT: higher efficiency of disinfection.

1 ex, 1 tbl

FIELD: veterinary science, veterinary disinfection.

SUBSTANCE: the suggested disinfectant should be applied for sanitation of animal and poultry objects. It contains acetic acid, sodium monochloroacetate, sodium dichloroacetate, sodium acetate, sodium glycolate, sodium chloride and water at their certain ratio. At concentration of 0.3-1.0% at exposure of 3 h this preparation causes 100% lethality in sanitary-representative microorganisms.

EFFECT: higher efficiency of disinfection.

2 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: the suggested disinfectant contains glutaric aldehyde, didecyldimethylammonium chloride, ethoxylated alcohol, propylene glycol and water at certain ratios. The method to treat objects, those of medicinal indications, among them, deals with treating an object with the above-mentioned disinfectant followed by keeping disinfectant on the object during the time and at temperature to provide disinfection and/or sterilization of the object followed by washing disinfectant off. The present innovation provides hermetic nature of elements and connective elements of flexible and rigid endoscopes, soldered and glued sites in the course of disinfection.

EFFECT: higher efficiency of application.

10 cl, 3 ex

Disinfectant // 2249466

FIELD: medicine.

SUBSTANCE: the present innovation deals with disinfecting and sterilizing different objects. The suggested disinfecting preparation contains aqueous solution of oxidants obtained due to treating in anode chamber and low-molecular alcohol. The latter should be introduced into the above-mentioned solution of oxidants at the quantity of 0.1-5.0 volumetric % or after processing in anode chamber before application or - into initial solution before processing in anode chamber. The suggested preparation is of increased biocide activity at no increase in concentration of active substances.

EFFECT: more broadened functional capacities.

3 cl, 5 ex, 1 tbl

FIELD: chemical methods for production of silver ion-containing disinfectant solution.

SUBSTANCE: silver concentration is carried out by chemical reaction and simultaneous dissolution in distilled water at room temperature of composition containing (g/l): powder citric acid 1.0-5.0; silver oxide 0.3-1.5; hydrogen peroxide 1.5-7.5; and balance: distilled water. Time of silver concentration is limited by reaction time.

EFFECT: non-toxic high stable disinfectant aqueous solution useful both in field conditions and stationary domestic objects.

2 cl, 3 ex

Disinfecting agent // 2246953

FIELD: sanitary and hygienic materials.

SUBSTANCE: water-based disinfecting composition contains water-soluble cyclodextrin-iodine inclusion complex and/or derivatives thereof, iodine, iodide ion source, organic solvent, and, if necessary, surfactant and/or functional additive, all in specified proportions. Agent possesses wide spectrum of antimicrobial effects at low concentrations. It does not irritates skin, shows no toxicity, and is stable upon storage within a wide temperature range.

EFFECT: increased choice of high-efficiency disinfecting agents.

19 cl, 2 tbl

FIELD: animal science, veterinary science.

SUBSTANCE: the suggested composition for disinfecting objects of animal science includes dispersion dyestuff and active substance as conservant-disinfectant FH and, additionally, cationic surface active substance at certain quantitative ratios of components. Moreover, disinfecting composition contains either alkyldimethylbenzylammonium chloride, or cetylpiridinium chloride or hexidine chloride as cationic surface active substance. This innovation provides increased terms of residual biocidal (bactericidal and fungicidal) activity.

EFFECT: higher efficiency of disinfection.

1 cl, 11 ex

FIELD: animal science, veterinary science.

SUBSTANCE: the suggested composition includes either chalk or slaked lime, additionally, it may contain conservant-disinfectant FX at certain ratios of components. The innovation provides increased terms of residual biocidal (bactericidal and fungicidal) activity.

EFFECT: higher efficiency of disinfection.

8 ex

Disinfecting agent // 2261089

FIELD: medicine.

SUBSTANCE: invention relates to a disinfecting agent involving ethyl alcohol and an active additive that comprises additionally acetaldehyde, diethyl phthalate and glycerol and/or propylene glycol in the following ratio of components, wt.-%: ethyl alcohol, 69-94; active additive as measured for the main substance 0.0004-0.12; acetaldehyde, 0.5-1.0; diethyl phthalate, 0.08-0.20; glycerol, 0.00-0.15, and/or propylene glycol, 0.00-0.0001, and water, the balance. The disinfecting agent comprises catamine AB or biopag D as an active additive as measure for the main substance in the amount 0.025-0.12, or benzyl benzoate (3-5 mg/l) and menthol (1-3 mg/l). The disinfecting agent can be used as a cutaneous antiseptic agent (in the presence of glycerol and/or propylene glycol as components) and disinfecting agent for treatment of surfaces. Invention provides the effective treatment of surfaces in infectious of bacterial, viral and fungal etiology. Agent doesn't irritate hand skin.

EFFECT: improved and valuable properties of agent.

4 ex

FIELD: chemical technology.

SUBSTANCE: invention relates to a method for preparing disinfecting agent as derivative of polyhexamethylene guanidine. Method involves thermal polycondensation reaction of hexamethylenediamine and guanidine hydrochloride for two stages. At the first stage hexamethylenediamine is added to guanidine hydrochloride melt up to the molar ratio = 1:1 and mixture is subjected for control thermostatically up to ceasing ammonia evolving. At the second stage hexamethylenediamine excess is added to formed melt of polyhexamethylene guanidine chloride that is taken as measured its molar ratio with guanidine hydrochloride = 2:n wherein n means degree of polymerization that is calculated based on molecular mass of terminal groups of polyhexamethylene guanidine derivative obtained at the first stage by the following formula: n = [%Cl]*Mk/(35.45-[%Cl]*177.678) wherein %Cl means the relative content of chlorine by mass in derivative of polyhexamethylene guanidine; Mk means molecular mass of terminal groups of polyhexamethylene guanidine derivative and at Mk = 17.034 the relative content of Cl is determined in reaction mixture before addition of hexamethylenediamine excess, and at Mk = 215.382 the relative content of Cl is calculated in the composite of polyhexamethylene guanidine derivative formed at the second stage. Then the reaction mixture is subjected for control thermostatically at temperature 165-200°C up to ceasing ammonia evolving. The end product is prepared as polymer-analog of polyhexamethylene guanidine chloride. Invention provides reducing yield of the end product in water-insoluble fraction and to enhance bactericidal activity of disinfecting agent. Invention can be used in medicine and veterinary science, in treatment of natural and sewage waters, in processes for leather and fur tanning and in industrial and domestic conditions wherein bactericidal preparations are required.

EFFECT: improved preparing method.

1 tbl, 2 ex

FIELD: medicine, hygiene.

SUBSTANCE: invention relates to a disinfecting agent. Interpolymeric agent comprises the following ratio of components, wt.-%: polyguanidine base, 0.3-0.5; chlorine-containing polymer, 3.1-4.0; ethyl alcohol, 7-10; nefras, the balance. Polyalkylene guanidine base or polyoxyalkylene guanidine, or polyalkylene biguanide is used as a polyguanidine base. Mixture of chlorosulfonated polyethylene with chlorinated polyvinyl chloride taken in the ratio = (7.3-10.1):1 is used as a chlorine-containing polymer. Agent can be used for preventive prolonged disinfection in compartment with many people amount, for example, in medicine institutions, in objects of criminal-executive system, evacuation points, in apartments for culture-domestic aims, in homes, veterinary, food branch, trade, transport. Invention provides enhancing strengthening indices of polymeric cover and to expand field of its applying.

EFFECT: valuable properties of agent.

4 cl, 6 tbl, 14 ex

FIELD: medicine, in particular disinfectant for domestic and industrial compartments, surfaces, tools, transport.

SUBSTANCE: claimed composition contains mixture of tetramethylenediethylene tetramine with silver ninrate, stabilizer and silica gel. Tetramethylenediethylene tetramine is obtained by reaction of at 50-70°C for 60-80 min in absence of alkali, then formed solution is cooled to 18-20°C for 12-18 h.

EFFECT: disinfectant with improved quality, higher bactericide and virulicide action; method with increased yield.

2 cl, 4 tbl, 6 ex

FIELD: veterinary science, veterinary disinfection.

SUBSTANCE: the suggested disinfectant should be applied for sanitation of animal and poultry objects. It contains acetic acid, sodium monochloroacetate, sodium dichloroacetate, sodium acetate, sodium glycolate, sodium chloride and water at their certain ratio. At concentration of 0.3-1.0% at exposure of 3 h this preparation causes 100% lethality in sanitary-representative microorganisms.

EFFECT: higher efficiency of disinfection.

2 ex, 1 tbl

Up!