Method and means for carrying out specific immunotherapy of allergic disease patients having sensitization to gnat stings

FIELD: medicine.

SUBSTANCE: allergen is taken as aqueous salt solution of protein polysaccharide complex of 10000 PNU activity produced by extracting dry degreased gnat of Aedes aegypti with alkaline buffer solution at pH of 8.0-8.2 with following cleaning as means for specific immunotherapy means applicable to allergic disease patients having sensitization to gnat stings. Method involves applying allergen of 10000 PNU concentration in 10-fold dilution introduced to a patient beginning from a dose of 0.2 ml with 1-2 days long pause. Supporting doses of the allergen are administered to the patients before gnat flight season with 10-12 days long pauses.

EFFECT: high effectiveness of the allergen and treatment method.

3 cl

 

The invention relates to pharmaceutical industry and relates to preparation for specific immunotherapy (SIT).

Known allergen of the mosquito species Aedes aegypti for the diagnosis of susceptibility to mosquito bites. Allergen derived from dry fatless mosquitoes extraction alkaline buffer solution at pH 8.0 to 8.2, followed by centrifugation at 6000 rpm for 30 minutes at 2-8°C. the Allergen is a transparent liquid from light yellow to brown, 1 ml of which the content of protein nitrogen is 10000+2000 PNU/ml

The present invention is the use of specified allergens for specific immunotherapy of patients with allergic diseases with hypersensitivity to mosquito bites.

To achieve the objectives of the proposed group of inventions, United by a common inventive concept.

The proposed application of the allergen, which is an aqueous saline solution of protein-polysaccharide complex with an activity of 10,000 PNU, obtained by extraction of dry fat-free mosquito species Aedes aegypti alkaline buffer solution at pH 8.0 to 8.2, followed by purification as a means for specific immunotherapy of patients with allergic diseases with hypersensitivity to mosquito bites. A str is about allergen specific immunotherapy of patients with allergic diseases, with sensitization to mosquito bites allergen concentration of 10,000 PNU/ml in tenfold dilutions, by introducing the patient increasing doses of specific allergens from the bodies of mosquitoes, starting with a dose of 0.2 ml with an interval of predominantly 1-2 days. The main goal of SIT is to reduce hypersensitivity of the patient to the allergen mosquitoes to prevent allergic reactions to mosquito bites during flight insects. Patients before the season departure of mosquitoes can be conducted introduction maintenance doses of allergen with an interval of 10-12 days. To conduct SITH prepare a tenfold dilution of the allergen (initial concentration is 10,000 PNU/ml). The minimum concentration of the drug when conducting SIT is determined individually for each patient based on the results of skin Prick-titration tenfold dilutions of the allergen (10,000 PNU/ml to 0.001 PNU/ml). The initial dose of the allergen when conducting SIT corresponds to 0.2 ml of the drug concentration corresponding to skin titer - the minimum concentration of allergen, the first giving slabopolojitelen (+) reaction at Prick-titration. The schema may be modified for individual patients according to their individual sensitivity to the drug. The interval between injections can be increased depending on the tolerability of the drug to patients (local reactions in the form of the ISU is remii, itching and swelling). The introduction of supporting doses with an interval of 1 to 12 days before the season departure of mosquitoes.

Therapeutic drug-allergen from mosquitoes of the species Aedes aegypti is when SIT secure, if for all (100%) of the examined patients there is no General (fever, headache, hyperthermia etc) and local giperergicakie reaction (larger than the top, provided the established scheme accounting reactions) when conducting SIT this allergen.

Laboratory analyses of blood, urine should also confirm the absence of acute and chronic toxicity of therapeutic doses of the allergen from the mosquito species Aedes aegypti.

The results of the SITH allergen of the mosquito species Aedes aegypti was evaluated at the end of the season departure of mosquitoes. Clinical manifestations in the bite of mosquitoes at the end of the season was evaluated on the basis of objective examinations of patients the doctor-allergist (not less than 2 times in a season of flight of mosquitoes). The severity of the clinical manifestations of the bites were registered by a conventional 5-point scale: excellent effect - 3 points, good - 2, satisfactory - 1. Therapeutic drug - allergen from mosquitoes of the species Aedes aegypti was found to be effective, there has been a decrease in clinical manifestations of Allergy to mosquito bites or complete absence of these symptoms (1 to 3 points). therapeutic effect was not less than 60% of patients. The effectiveness of the applications of the drug were also assessed by the level of synthesis of specific IgG antibodies by ELISA. Increased titers of specific IgG-unit-antibodies in samples of blood sera of patients with sensitization to mosquito bites after the course SIT testifies to the effectiveness of allergen.

To study therapeutic value of the allergen from the mosquito species Aedes aegypti when SIT was tested on 30 patients with Allergy to mosquito bites in age from 18 to 50 years. The efficiency of the SITH allergen from mosquitoes was evaluated according to common criteria evaluation: excellent effect, good, satisfactory, unsatisfactory. Improvement was noted in all treated patients. Excellent results were obtained in 30% of patients, good in 40%, satisfactory - 30%. Unsatisfactory (no effect) no results found. The application of the allergen from mosquitoes to SIT patients allergic diseases with IgE-mediated allergies helped to identify high efficiency allergen and the schemes of the drug (70%). When pharmacotherapy is unable to receive a specified treatment effect.

1. The application of the allergen, which is an aqueous saline solution of protein-polysaccharide complex with an activity of 10,000 PNU, obtained by extraction of dry fat-free mosquito species Aedes aegypti alkaline buffer solution at pH 8.0 to 8.2 with subsequent treatment, as a means for specific immunate the FIPA patients with allergic diseases, with hypersensitivity to mosquito bites.

2. Method specific immunotherapy of patients with allergic diseases with hypersensitivity to mosquito bites, characterized by the fact that they use the tool according to claim 1, the allergen concentration of 10,000 PNU/ml in tenfold dilution is administered to the patient, starting with a dose of 0.2 ml with an interval of predominantly 1-2 days.

3. The method according to claim 2, in which before the season departure of mosquitoes can be entered maintenance dose of allergen with an interval of 10-12 days.



 

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