Diagnosis method for distinguishing the cases of therapeutically resistant reactive depression
SUBSTANCE: method involves evaluating patient state using Hamilton scale, considering leukocyte formula of general clinical blood analysis to determine leukocyte and monocyte number and determining whole blood infrared spectroscopy absorption spectrum values in infrared analyzer within 30 s in the bandwidth of 3085-2832 cm-1 and 1543-1425 cm-1, respectively. Mean value of depression intensity being equal to 19.0±2.1 points, mean absorption values being equal to 40.5±3.2% and 43.4±2.9% in the bandwidth of 3085-2832 cm-1 and 1543-1425 cm-1, respectively in combination with immunity characteristics like monocytosis>5% and lymphocytosis>25% leukocyte formula of general clinical blood analysis being observed, therapeutically resistant reactive depression cases are to be diagnosed.
EFFECT: high accuracy of diagnosis.
The invention relates to medicine, namely to psychiatry and neurology. Depression is one of the most common disorders in psychiatric and somatic practice. More than one-third of patients with depression find resistance to timoanalepticheskoe therapy than due to the need to identify ways that timely and accurate predictions of refractoriness to therapy.
The method improves the accuracy of diagnosis of resistance in reactive depression by determining the biochemical and immunological markers are predictors of refractoriness to antidepressant therapy. During testing identifies persons with pathobiochemical and immunological signs of disorders of the nervous system, which will continue to occur or already manifest at the time of the survey depressive symptoms that are resistant to modern antidepressant psychopharmacological agents. The method provides high accuracy of definition of persons with specified symptoms to clarify the dynamics of the situation in the clinic.
As a prototype of the present invention applicants propose a clinical method for the diagnosis of treatment-resistant depression on the Hamilton depression rating scale [thesis for the academic Art. CMN Pokrovsky became popular Therapy re istotnie depression and metabolic disorders phospholipids (clinical pathobiochemistry aspect). - Tver, 1999. - 25 S.].
Determine the severity of depression on the Hamilton depression rating scale calculates the amount of points. Depression is considered resistant if for two consecutive courses adequate monotherapy pharmacologically different (according to the chemical structure and biochemical effects) antidepressants after 6-8 weeks to acknowledge the absence or lack of clinical effect. During this period, again assessing depression on the Hamilton depression rating scale, and by reducing the number of points less than 50% from baseline classify depression as treatment-resistant. Adequate treatment of reactive depression includes the appointment of a tranquilizer and antidepressant according to clinical indications and characteristics of the spectrum of its psychotropic, neurotropic and somatotropic activity, the use of an appropriate range of doses with growing up with the ineffectiveness of therapy two weeks before maximum or parenteral introduction of and compliance with the terms of course duration of at least 3-4 weeks. This clinical diagnosis method standardizes the group resistant to timoanalepticheskoe therapy of depressed patients, and offers a practical physician procedure and provides continuity in the patient's treatment.
The disadvantages of the prototype, VZG the poison authors, following:
1. The method is subjective, depends largely on the level of specialist training.
2. Cannot identify the intimate neurobiochemical resistance mechanisms of reactive depression therapy.
3. Requires a long time.
In contrast to the above method of the prototype, the proposed new method reveals the average results for a cohort of treatment-resistant patients with reactive depression. Determine the severity of depressive symptoms on the Hamilton depression rating scale (21), which for reactive treatment-resistant depression (TRD) is the average 19,0±score of 2.1. Count leukocyte formula of the General analysis of blood, diagnostically important data monocytosis > 5% and limfozitoza > 25%. Identify indicators absorption (%) infrared spectrum of whole blood in the range 3085-2832 cm-1- 40,5±3.2% and in the range 1543-1425 cm-1- 43,4±2,9%, while the norm is in the range 3085-2832 cm-1- 25,6±0,6%, in the range 1543-1425 cm-1- 29,1±1,1% (table. 1).
The method is as follows. Clinical assessment is carried out in accordance with the ICD-10 criteria for a protracted reactive depression and dysthymia. Determine the severity of depressive symptoms on the Hamilton depression rating scale (21), which sostav the em average 19,0± 2.1 points.
Modern research shows that the immune system by organ-specific immune responses humoral and cell type directly involved in the regulation of nervous systems. In particular, revealed the interactions immunological and clinical parameters in depressive disorders. However it is noted that with the increase in the limitations of mental illness, the role of the immune system in the pathogenesis of depression, and therefore increases the risk of immunodeficiency.
In order to identify immunological markers of resistance to depression therapy leukocyte count formula General clinical blood analysis. Material for General clinical blood analysis is capillary whole blood.
Prepared smear is fixed with methyl alcohol for 5 min, and then stained by Romanovsky - Giemsa. Paint standard, incorporates Azur II, water-soluble yellow eosin, methyl alcohol and glycerin. The working solution of the paints is prepared by dilution of 3 drops of finish paint 1.0 ml of distilled water. The duration of the painting 40 minutes. Stained smears examined under a microscope with oil immersion system (eyepiece 10, the lens 90). To count leukocyte use 11-key counter, record the number of each type of leukocytes in percent.
Detected in the cohort of treatment-resistant patients with reactive depression the average performance of monocytosis > 5% indicate tension phagocytic immunity, lymphocytosis > 25% shows the defect cell component of the immune system, which confirms the heterogeneity and multi-level neurobiochemistry of resistance mechanisms of depression to timoanalepticheskoe therapy.
The definition of resistance in depression, the authors carried out by blood tests by infrared spectroscopy method (X) on the hardware-software complex "Icarus" 9/1 (patent No. 2137126 from 10.09.1999, certification No. 5745). The material for the X - integral capillary blood volume of 0.1 ml, measured by the pipette is placed in the original cell and subjected to spectrophotometrically on agriculture "Icarus" 9/1, 9 optical channels with wavelengths of from 3500 to 930 cm-1within 30 seconds the Study was conducted on all 9 channels, however, the most significant were the key figures in the ranges of channels 3085-2832 cm-1and 1543-1425 cm-1. The device records the percentage transmittance of the optical medium, which is a value inversely proportional to the extinction of the solution. Output information is the absorption index (%) infrared spectrum radiation in any given interval of the spectrum in use is e 30 C. The data obtained in the study of blood in the infra-red spectrum analyzer "Icarus" 9/1, obrabatyvat by the standard method and identify the average results for a cohort of treatment-resistant patients with reactive depression. In the ranges X 3085-2832 cm-1and 1543-1425 cm-1identify indicators absorption and calculate the average absorption. Normal averages absorption in the range X 3085-2832 cm-1- 25,6±0,6, in the range 1543 -1425 cm-1- 29,1±1,1. In treatment-resistant depression is the average absorption in the range X 3085-2832 cm-1- 40,5±3,2%, in the range 1543 -1425 cm-1- 43,4±2,9%.
Change indicators infrared spectrum of blood (in %, M±m) and indicators of immunity in patients with reactive treatment-resistant depression.
|The boundaries of the spectral region, cm-1||Absorption in % $ OK||Treatment-resistant reactive depression||Components, as defined by the IRS in the blood||Indicators of immunity in treatment-resistant reactive depression|
|3085-2832||25,6±0,6||40,5±3,2**||phospholipids, cholesterol esters, triglycerides||The content of lymphocytes more than 25% *||The content of monocytes more than 5% *|
|1543-1425||29,1±1,1||43,4±2,9**||methylene and methyl groups contained in all components of membranes|
|Note: *-differences from the norm are significant, p <0,05; ** -differences from the norm are significant, p <0,001|
Diagnosis of reactive treatment-resistant depression using X blood of patients based on the detection of high performance infra-red radiation absorption on the channels where the tested chemical bond characteristic of phospholipids and virtually all components of biological membranes (3085-2832 cm-1and 1543-1425 cm-1) [Kesler I. Methods of infrared spectroscopy in chemical analysis. - M.: Mir. - 1964. - 257 C.]. Significant improvements absorption of IR radiation blood of patients in these ranges indicates the increase in the concentration of sphingomyelin, phosphatidylserine and other phospholipid fractions in the blood. Detected with X increasing the concentration of sphingomyelin and other fractions of phospholipids in the blood of patients with reactive resistant depression is associated with severe damage cleto is different membranes. The fact that when mental illness changes the content of phospholipids in the blood, it has been proved in several studies [Morkovkina V.M., Cartelised A.V. Patagonia schizophrenia. - M.: Medicine - 1988. - 255 S.]. It is known that phospholipids are the major structural and functional component of biological membranes, are directly involved in the processes of the transmembrane transfer of all metabolites involved in the processes of excitability of the cell, their energy metabolism, protein biosynthesis, and also act as second messengers. In addition, they act as antioxidants, preventing excessive degree of free oxidation of biologically active substances, as well as proektiruemij pathological effects of peroxides on vital structures of the body. The importance of these points are particularly obvious, as is proved by the intensification of the processes of lipid peroxidation (LPO) in different Genesis of mental illness. It was found that chronic emotional stress, underlying depressive suffering, leads to a state of metabolic intracellular hypoxia due to the predominance of the processes of glycolysis over oxidative phosphorylation, i.e. anaerobism on tissue respiration. Under these conditions, impaired utilization of glucose (glycogen), h is about in turn leads to blocking the formation of amerosport of sphingosine - ceramide. Thus due to the activation of specific transferring enzyme produces increased amounts CM (part of which is spent on increasing the synthesis of the FE and FS), which is gipolipidemiceski source of energy depot in the conditions of intracellular hypoxia. You should also take into account the interconversion of phosphatidylethanolamine and phosphatidylserine by decarboxylation reactions of the latter. This reaction proceeds predominantly in the mitochondrial membranes, which confirms the data exclusively about the importance of aminecontaining phospholipids in the processes of oxidative phosphorylation [Racer E. Bioenergetic mechanisms: new perspectives /Ed. Skulachev V.P.), 1979. - 217 C.]. Thus, detected using infrared spectroscopy, the change in optical density of the blood of patients with treatment-resistant reactive depression in the optical spacing characteristic of phospholipids and other components of biological membranes, associated with the characteristic depression of hypoxic processes and as a consequence pronounced changes in biomembranes. Due to the above identified significant improvements absorption of infrared radiation by the blood of patients in the specified ranges, showing the typical resistant Dept is ASCII increasing the concentration of SFM, FAA and FS in the blood, can be used to diagnose reactive treatment-resistant depression.
Study included 75 patients with reactive depression, 40 women and 35 men from 22 to 50 years, average age 35±3.6 years. The results timoanalepticheskoe therapy patients were divided into responders (36 patients), found a positive trend, and the responders (39 patients), have significant positive shifts in the process of therapy is not marked. Clinical studies met the ICD-10 criteria, supplemented by depression scale M Hamilton (21 issues). The control was a group of 50 healthy individuals of comparable age and sex. Laboratory studies included a leukocyte count of clinical blood analysis, combined with an average absorption (%) infrared spectrum of whole blood in the range 3085-2832 cm-1and in the range 1543-1425 cm-1.
The advantages of this method for the diagnosis of treatment-resistant reactive depression:
1. The proposed method allows to assess the dynamics of paired species metabolism in organ, tissue, cellular and subcellular levels.
2. When used in the clinic will discover pathobiochemical and immunological markers of treatment resistance reactive depr the hurt.
3. The use of the claimed method will allow you to control biochemical and immunological levels of the patient's condition, objectivesare clinical assessment of monitoring the effectiveness of treatment.
4. The diagnostic method is easy to use, suitable for use in the clinical setting.
CLINICAL EXAMPLE. Patient N., 43 years. Case history No. 615. Diagnosis: Dysthymia. The disease is associated with long objectively insoluble stressful situation. Clinically detects signs of a depressive episode moderate degree. The severity of depression scale M Hamilton is 21.5 points. Clinical blood analysis revealed lymphocytosis - 27% and monocytosis - 6%. A blood sample was investigated X in the range 3085-2832 cm-1the rate of absorption of 42.8%, in the range 1543-1425 cm-1absorption - of 45.7%. On the basis of the obtained data revealed treatment-resistant reactive depression.
Method for the diagnosis of treatment-resistant reactive depression, including the assessment of the condition of the patient on the Hamilton depression rating scale, characterized in that reveal the presence of monocytosis and limfozitoza, within 30 to determine the performance of infrared absorption spectroscopy of blood in the infra-red spectrum analyzer in the range 3085-2832 cm-1and 1543-1425 cm-1R is schityvat average absorption and with the average severity of depression 19,0±score of 2.1, the value of the average absorption 40,5±3,2% and 43.4±2.9% in the ranges 3085-2832 cm-1and 1543-1425 cm-1accordingly, in combination with indicators of immunity in the form of monocytosis >5% and limfozitoza >25% in the leukocyte formula common blood diagnosed with treatment-resistant reactive depression.
SUBSTANCE: method involves evaluating patient state using Hamilton scale, considering leukocyte formula of general clinical blood analysis for determining infrared spectroscopy absorption spectrum values within 30 s in the bandwidth of 3085-2832 cm-1 and 1543-1425 cm-1, respectively. Mean value of depression intensity being equal to 21.0±2.8 points, mean absorption values being equal to 32.3±1.8% and 35.0±2.2% in the bandwidth of 3085-2832 cm-1 and 1543-1425 cm-1, respectively and normal lymphocyte and monocyte content being observed, reactive depression is to be diagnosed. Mean absorption values being equal to 40.5±3.2% and 43.4±2.9% in the bandwidth of 3085-2832 cm-1 and 1543-1425 cm-1, respectively in combination with immunity characteristics like monocytosis>5% and lymphocytosis>25% leukocyte formula of general clinical blood analysis being observed, therapeutically resistant reactive depression cases are to be diagnosed.
EFFECT: high accuracy of diagnosis in differentiating cases showing positive changes as response to pharmacotherapy from therapeutically resistant cases.
FIELD: analytical methods in biology.
SUBSTANCE: invention relates to chemical analysis methods for biological materials and, in particular, concerns a method for determining acetylcholine in biological specimens consisting in incubation of a specimen, centrifugation, take-off of supernatant, preparation of alkaline hydroxylamine reagent by adding equal volume of 3.5 n NaOH to hydroxylamine chloride solution, mixing of thus prepared reagent with test specimen, and addition of iron chloride solution followed by measurement of optical density. Invention is characterized by that, before addition of 3.5 n NaOH, hydroxylamine chloride solution is mixed with 10-14% solution of Na2CO3, incubation of specimen is performed after the latter is mixed with alkaline hydroxylamine reagent at 1:2 ratio for 90-120 min at 33-37°C, centrifugation is preceded by adding aqueous hydrochloric acid solution, and 0.74 M iron chloride solution is added to taken off supernatant.
EFFECT: increased determination accuracy.
2 tbl, 3 ex
FIELD: veterinary medicine, biochemistry.
SUBSTANCE: the method deals with treating the smears with buffer mixture followed by washing with distilled water, drying, additional dyeing with 0.5%-methylene blue followed by washing, drying, detecting enzymatic activity by the quantity of dyed granules. The innovation enables to widen technological possibilities in the field of immunological investigations.
EFFECT: higher accuracy of detection.
5 dwg, 1 ex, 1 tbl
FIELD: veterinary medicine, biochemistry.
SUBSTANCE: the method deals with treating the smears with buffer mixture followed by incubation in darkness for 2 h, washing with distilled water, drying, additional dyeing with 0.5%-methylene blue followed by washing, drying, detecting enzymatic activity by the quantity of dyed granules. The innovation enables to widen technological possibilities in the field of immunological investigations.
EFFECT: higher accuracy of detection.
5 dwg, 1 ex, 1 tbl
FIELD: veterinary, biochemistry.
SUBSTANCE: claimed method includes smear preparation, fixing thereof, washing, embedding and incubation in medium containing buffer solution, distillated water, naphthylophosphate and neutral fast blue dye, followed by washing, addition dyeing and estimation of qualitative coloration.
EFFECT: enhanced processing capability; decreased efforts.
5 dwg, 1 tbl, 2 ex
SUBSTANCE: method involves determining averaged MMPI-pattern, vegetative regulation tendency, central hemodynamics type and prostaglandin concentration in blood serum. The averaged MMPI-pattern being increased, sympathetic vegetative regulation tendency being increased, blood circulation being of hypokinetic type and prostacyclin content being low with thromboxane content being high, EPH-gestosis is to be predicted.
EFFECT: high accuracy of early-stage prognosis.
3 dwg, 4 tbl
SUBSTANCE: the present innovation deals with treating cerebral diseases, Parkinson's disease being among them. One should measure biochemical and immunological blood values of a patient, the values of MAO-B thrombocytes activity, the values of erythrocytes activity, Cu/Zn - TFD, the level of neuromediators of glutamate and taurine in blood plasma and circulating immune complexes (CIC) in blood serum. To perform the impact with laser irradiation it is necessary to choose the patients with all, several or only one value in ranges given below: MAO-B 90-250 nM/mg or MAO-B 0-47 nM/mg, at the norm of 73+7 nM/mg, Cu/Zn - TFD 1.2-2.5 U/mg or Cu/Zn - TFD 0-0.8 U/mg, at the norm of 1.5 ± 0.05 U/mg, CIC low-molecular fractions 100-300 c.u., at the norm of 80 ± 5.0 c.u., CIC average-molecular fractions 48-150 c.u., at the norm of 39 ± 2 c.u., affected ratio of glutamate to taurine. Impact should be carried out intravenously with the help of a light guide at the power at its end being 1 mW and wave length of 632.8 daily for 5 d, every seance lasts for 20 min, then medicinal therapy should be fulfilled without laser impact by repeating the course of laser irradiation in a year. The method enables to increase the quality in treating Parkinson's disease due to stimulating the system of antiradical tissue protection, increased protective body reserves and correction of immune disorders.
EFFECT: higher efficiency of therapy.
FIELD: medicine, oncology.
SUBSTANCE: invention relates to a method for assay of efficacy on post-operative intrathecal chemotherapy of patients with intracerebral malignant tumors. Method involves determination of total protein level in cerebrospinal fluid before and after carrying out chemotherapy in autoliquor. Increase of the level of total protein by 5.6-6.3 times indicates on effectiveness of the post-operative chemotherapy. Method provides the objective evaluating dynamic of cytolysis of tumor cells that is the reliable method for estimation of efficacy of indicated treatment.
EFFECT: enhanced and improved method for treatment.
FIELD: veterinary medicine.
SUBSTANCE: the suggested method deals with sampling smears, their treatment, fixation, pouring and incubation in medium consisted of buffer solution, naphthyl phosphate and neutral strong blue B dyestuff, additional dyeing with neutral red dyestuff followed by qualitative dyeing. The innovation enables to decrease financial expenses and time.
EFFECT: higher accuracy of detection.
5 dwg, 1 ex, 1 tbl
FIELD: medicine, oncology.
SUBSTANCE: the present innovation deals with biochemical trials in the field of oncology. Moreover, before the onset of autohemotherapy in patients one should detect the content of thyroid hormones -triiodothyronine and thyroxine, and hypophysial hormone - thyreotropin. At its value ranged 166-408 one should predict efficiency of therapy, and at coefficient value ranged 34-106 - the absence or low efficiency of therapy. The present method enables to detect sensitivity of tumor to autohemochemotherapy before its onset, avoid application of inefficient medicinal remedies and their possible toxic impact and prevent groundless expenses of expensive preparations.
EFFECT: higher efficiency and accuracy of prediction.
2 ex, 1 tbl
SUBSTANCE: method involves placing 8-10 blood serum drops on Petri cup bottom, covering with the second Petri cup, placing into vacuum chamber under pressure of 0.1-0.5 mm of mercury column, T=+18-20°C, drying during 1-1.5 h and studying with microscope in passing light. Spiral aggregates of chain-oriented or rarefied one being detected, hyper-beta 2-microglobulinemia cases are to be diagnosed.
EFFECT: simplified diagnosis method.
FIELD: medicine, neurology.
SUBSTANCE: one should study subpopulation composition of blood formic elements - erythrocytes and their morphodensitometric parameters - integral value of local membranous curvature, index of spicularity and erythrocytic surface area. Chronic cerebral ischemia stage I should be diagnosed at values of percentage content of discocytes ranged 60-66.2±0.8%, spherocytes - 0.7-11%, leptocytes - 0.1-2.2%, echinodiscocytes - 0.4-4.6%, stomatocytes - 5.8-2.6%, integral value of local membranous curvature being above 4.0±1.3 up to 12.5±1.5, index of spicularity being above 0.1 up to 0.15, surface area being above 24.35-26.976. The second stage should be diagnosed at values of percentage content of discocytes ranged 48-59%, echinodiscocytes - above 4.6-9.3%, discospiculocytes - 0.3-0.4%, spiculocytes - 0.8-4.2%, integral value of local membranous curvature ranged 12.5±1.5 up to 34.0±, index of spicularity being above 0.15 up to 0.3, erythrocytic surface area being above 26.976 up to 30.12. The innovation enables to objectively subdivide early stages of chronic cerebral ischemia.
EFFECT: higher accuracy of diagnostics.
4 dwg, 3 ex, 2 tbl
SUBSTANCE: one should register the values of pH and Redox potential (Red) of liquid means in bio-objects simultaneously. It is necessary to plot a graph ▵pH/▵Red according to fluctuations of variation values in measured parameters at their temporal diagram to transfer it into a columnar one where the height of every column is proportional to the area of this graph between neighboring measurements, correspondingly. According to the ratio of area sums of positive columns to that of negative ones during a certain period of time ( from a minute- to a year-long ones) one should conclude upon a state in a bio-object. The value ranged 0.5-2 is considered to be a standard. The method enables to provide efficient control for a two-phase systemic process.
EFFECT: higher efficiency and accuracy of diagnostics.
1 cl, 9 dwg, 5 ex, 1 tbl
SUBSTANCE: as initial material one should apply urine of pregnant women at late terms of pregnancy or that of gestosis-suffering pregnant women at late terms of pregnancy. Saline protein fractioning should be carried out with ammonium sulfate of 40% saturation, this solution should be exposed for 8-10 h followed by centrifuging at 7000 rot./min for 2025 min. Residue diluted with 0.15 M sodium chloride solution should be salted out due to dialysis against 0.025 M phosphate buffer at pH - 7.4. The method provides more simplified technique and shortened terms for obtaining the preparation desired.
EFFECT: higher efficiency.
1 cl, 1 dwg, 6 ex
FIELD: veterinary science.
SUBSTANCE: the method deals with growing bacterial mass of Salmonella followed by its deposition, treating with 12%-sodium chloride solution, autoclaving at 121 C for 20 min, supplementing detergent, sedimentation due to centrifuging at 8-9 thousand rot./min for 30-35 min followed by applying supernatant (lipopolysaccharide-protein complex) to sensitize ram's formalinized erythrocytes. The method is considered to be economical, available and enables to obtain active and specific erythrocytic diagnostic kit.
EFFECT: higher efficiency.
FIELD: medicine, pulmonology.
SUBSTANCE: one should evaluate granulocyte-bound lymphocytes (GBL) based upon GBL level consisted of 1 lymphocyte and 2 granulocytes (GBL2), differential diagnostics should be performed due to applying discriminant equation based upon measured GBL2 value. The method has been applied in 22 patients with chronic pneumonia: correct diagnosis was determined in 85% cases.
EFFECT: higher efficiency of application.
FIELD: medicine, diagnostics.
SUBSTANCE: one should study blood components to detect anticoagulant-fibrinolytic activity. Moreover, patient's blood should be sampled: in whole blood one should detect the presence of affected erythrocytes and evaluate the quantity of thrombocytes, in plasma it is necessary to study the activity of antithrombin III, XIIa-dependent fibrinolysis, the content of soluble fibrin-monomeric complexes, in blood serum of the sample taken one should detect the concentration of urea, creatinine, sodium, albumin, total cholesterol and the activity of aspartate aminotransferase, moreover, one should calculate integral value of renal-hepatic deficiency, to put corresponding point for the degree of parameters under testing, then one should calculate integral value of disseminated intravascular clotting (IVDIC) and at its value being 6.3 U and more DIC-syndrome should be diagnosed, moreover, at IVDIC value ranged 6.3-10.1 U it is possible to diagnose latent DIC-syndrome, at 10.2-14.6 - subacute DIC-syndrome and at 14.7 and higher - acute DIC-syndrome should be concluded.
EFFECT: higher accuracy and efficiency of diagnostics.
4 ex, 2 tbl
FIELD: analytical methods in medicine.
SUBSTANCE: method comprises mixing blood sample to be examined with anticoagulant, placing resulting solution into vertically oriented capillary, and measuring, with equal time intervals, height of erythrocyte-free plasma layer. More specifically, blood with anticoagulant is poured by means of automatic dispensing means into hematocrit capillary, lower end of which is tightly sealed, capillary is placed vertically in centrifuge pocket, and measurement of the height of erythrocyte-free plasma layer is performed, with equal time intervals, at angular velocity not higher than 50 ppm over a specified period of time. From data obtained, one determines maximum erythrocyte sedimentation velocity value and plots corresponding graph. Proposed device contains pockets for vertically positioning capillaries with test blood dose and measuring means as well as above-mentioned centrifuge having removable rotor mounted horizontally on motor shaft and control block enabling operation of centrifuge in step-running mode and in continuous-rotation mode. Rotor is provided with peripherally arranged pockets for accommodating at least 90 capillaries.
EFFECT: increased determination reliability.
7 cl, 4 dwg, 1 tbl
FIELD: medicine, phthisiology.
SUBSTANCE: one should detect the activity of myeloperoxidase (MP) and the content of cationic proteins (CP) in blood phagocytes and at CP content being above 105 c.u. at normal and increased MP activity one should diagnose sarcoidosis, and at CP content being below 105 c.u and decreased MP activity - pulmonary tuberculosis. The innovation provides more simplified way for differential diagnostics.
EFFECT: higher accuracy of diagnostics.
2 ex, 2 tbl
FIELD: medicine, in particular radiobiology.
SUBSTANCE: sheep native erythrocytes are sequentially treated with glutaric aldehyde, human blood serum albumin and 1 % cesium chloride solution. Claimed marker makes it possible to detect cesium nuclide in body of subject being irradiated by low doses over long period after irradiation.
EFFECT: specific marker for detection of cesium nuclide in blood after effecting of low-dose radiation.
3 ex, 3 tbl
SUBSTANCE: invention relates to laboratory methods for blood analysis. Plasma is dropped in copper sulfate solution with density 1.023 g/cm3, not above, and time for drop falling on bottom of graduated cylinder with column height 243 mm is measured. The blood plasma density value is calculated by the formula:
wherein is the unknown blood plasma density (g/cm3); is copper sulfate solution density measured by areometer (g/cm3); t is average falling time of plasma drop in the copper sulfate solution (as seconds); 0.260130126 and 0.00290695 are correction coefficients. Temperature of plasma and copper sulfate solution is 20oC. Method is simple and suitable and allows carrying out analysis of small volumes of blood plasma and to reduce analysis time.
EFFECT: improved assay method.