Method for manufacturing a preparation for stimulating animal bodies out of drone bee larvae

FIELD: veterinary science.

SUBSTANCE: the present innovation deals with medicinal preparations for increasing total and specific stimulation of animal body. The suggested preparation should be obtained out of drone bee larvae of different age. From the cells of drone bee brood one should cut off convex wax caps with a special knife for opening honeycombs, then due to light knocking one should remove larvae out of the cells into a cuvette to be put into vials. Larvae should be frozen and homogenized, moreover, as a stabilizing medium one should apply 5%-glucose solution at 1:10 ratio at addition of 5%-chloroform solution or 2%-salicylic acid followed by sublimation to be finally poured into vials per 5 ml. Moreover, sublimation should be fulfilled in vacuum chamber at temperature of shelves' heating up to 30-45 C. At achieving preparation temperature of 20-22 C shelves' temperature should be decreased up to 25 C to perform final heating for 22-24 h. The method provides increased total and specific stimulation of animal body due to applying cheaper and more available biostimulant.

EFFECT: more simplified method of manufacturing.

3 ex

 

The technical field to which the invention relates.

The invention relates to veterinary medicine, in particular to medicines to improve General and specific stimulation of the animal organism.

The level of technology

Known method of preparing tissue suspensions from parenchymatous organs (liver, spleen, testes, kidneys, and so on), which can be easily processed. After petisteho preservation at a temperature of 2-4°With, the fabric is washed with boiled water, weighed, ground in a sterile homogenizer and additionally pound in a porcelain mortar-homogenizer with a gradual addition of saline solution (1 g of tissue 2-3 ml). Thus prepared, the mass leave for two hours at room temperature and then for 30 minutes put on a water bath at 60-80°C. thereafter, the mass is filtered through 2-3 layers of sterile gauze and the filtrate poured into ampoules or vials. The sealed ampoules and vials stoppered and autoclave for 60 minutes at 120°. Then the vials poured paraffin or wax, paste labels (see Iaalay. Tissue therapy in veterinary medicine. M: Symptoms, 1960, s).

The disadvantage of this method is the high cost of materials, complexity and multi-stage.

A known method for preparation of the biostimulator for feeding the tics, providing an introduction to the basic diet of the biostimulator, prepared by cooling at a temperature of 2-4°C for 4-5 days, dilution with water in a five-fold volume and cooling during the day, followed by autoclaving the resulting mass. At the same time as the biostimulant use egg mass, and then introduce it in the diet in quantities of 0.2-0.3 ml per 1 kg body weight every 6 to 7 days (see A.S. USSR №1530163, class. And 23 To 1/16).

The disadvantage of this method is not sufficiently high effect of increasing the General and the specific resistance of the organism.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is the method of preparation of the drug for stimulation of the human body of the larvae of drones, including the collection of larvae, drones, their homogenization with the addition of a stabilizing environment (see "Harvesting larvae of drones, Vigliadiw, Maikovich, Institute of beekeeping, W. Beekeeping, No. 3, 2003, pp.52-54).

The disadvantage of this method is the short retention time of the drug.

Disclosure of inventions

The technical result that can be achieved using the proposed method is to increase General and specific stimulation of the animals, getting more cheap and available BIOS is emulator, simplification of the method of preparation.

The technical result is achieved by the method of preparation of the drug to stimulate the animals of the larvae of drones, including the collection of larvae, their homogenization with the addition of a stabilizing environment, while before homogenization larvae drones freeze at temperatures (-4; -5° (C) for 12-14 hours, then thawed to a temperature of +18°and homogenized for 3-5 minutes, while as a stabilizing environment of the use of 5% glucose solution at a ratio of 1:10 with the addition of a 5% solution of chloroform or 2% salicylic acid with subsequent sublimation, moreover, the sublimation is carried out in a vacuum chamber at a temperature of heated shelves up to 30°C-45°while when the temperature of the drug 20°-22°With the temperature of the shelves is reduced to 25°and conduct final drying for 22-24 hours.

The method of preparation of the drug to stimulate the animals from larvae drones

The drug is made from larvae drones of different ages. Which cells of the drone brood is cut convex wax caps, using the knife for printing process. Then gently tap the beat out their larvae from cells to clean the cell (pan), collected in the tank (volume of 50-200 ml), then larvae drones freeze at temperatures(-4; -5° (C) for 12-14 hours, then thawed to a temperature of +18°and homogenized for 3-5 minutes, while as a stabilizing environment of the use of 5% glucose solution at a ratio of 1:10 with the addition of a 5% solution of chloroform or 2% salicylic acid with subsequent sublimation, poured into 5 ml vials, and the sublimation is carried out in a vacuum chamber at a temperature of heated shelves up to 30°C-45°while when the temperature of the drug 20°-22°With the temperature of the shelves is reduced to 25°and produce a final drying for 22-24 hours.

The implementation of the invention

Examples of specific performance experiences of carrying out the method of preparation of the drug to stimulate the animals of the larvae of drones.

Experience No. 1. With cells of the drone brood is cut convex wax caps, then gently tapping beat their larvae from cells to clean the cell (pan), collected in the tank (volume of 50-200 ml). When this refrigerator with loaded proven, for example, 9-shelf bookcase pre-cooled to -20°S, cassettes drug is establish on the shelves of the bookcases, the temperature sensors of the drug set in the cassette, brought from the filling of the latter. After download install the freezing temperature (-30° (C) and include fridge, freezer etc is the W hold for 5-10 hours, then the product is thawed to a temperature of +18°and homogenize for 1-2 min, at the same time as stabilizing environment of the use of 5% glucose solution at a ratio of 1:10 with the addition of a 5% solution of chloroform followed by sublimation and sublimation is carried out in a vacuum chamber at a temperature of heated shelves to (20°25°C), while when the temperature of the drug 15-18°the temperature was lowered to 15°and conduct final drying for 15-20 hours.

The resulting preparation can not withstand the prescribed retention period is 2 years, when the use of animals does not give a high effect of improving General and specific stimulation of the organism.

Experience No. 2. Conduct similar experience No. 1, but the freezing is carried out at a temperature (-4; -5° (C) for 12-14 hours, then thawed to a temperature of +18°and homogenized for 3-5 minutes, while as a stabilizing environment of the use of 5% glucose solution at a ratio of 1:10 with the addition of 2% salicylic acid, with subsequent sublimation, poured into 5 ml vials, and the sublimation is carried out in a vacuum chamber at a temperature of heated shelves up to 30°C-45°While when the temperature of the drug 20°-22°With the temperature of the shelves is reduced to 25°and conduct final drying for 22-24 hours.

The resulting preparation represents the t of themselves loose, light brown paste which is prepared tablets that dissolve easily in sterile saline or distilled water, which is injected intramuscularly or give into the animal.

The resulting preparation in these parameters can withstand the prescribed retention period is 2 years and when using animals, for example pigs, increased immune system and increased the weight, and the mink litter.

Experience No. 3. Conduct similar experience No. 1, but the freezing is carried out at a temperature (-5° (C) for 15-20 hours, followed by thawing to a temperature of +18°and homogenization within 6-10 minutes. As a stabilizing environment of the use of 5% glucose solution at a ratio of 1:10 with the addition of a 5% solution of chloroform or 2% salicylic acid with subsequent sublimation and sublimation is carried out in a vacuum chamber at a temperature of heated shelves to 46°-55°while when the temperature of the preparation 35°S-40°C, the temperature of the shelves is reduced to 30°and conduct final drying for 25-30 hours.

The resulting preparation in these parameters was too crumbly and not packaged in pills, prescribed retention period has not passed, when fed to animals not given a high effect of improving General and specific stimulation of the organism.

The proposed image is a buy compared to the prototype and other known technical solution has the following advantages:

- the drug has the effect of tissue therapy;

- improves General and specific stimulation of the body;

- simplified method of manufacture;

- cheaper to manufacture;

the drug can be administered p/,/m and inward.

- has a shelf life of 2 years.

The method of preparation of the drug to stimulate the animals of the larvae of drones, including the collection of larvae, drones, their homogenization with the addition of a stabilizing medium, characterized in that before homogenization larvae drones freeze at temperatures (-4; -5°C)for 12-14 h, and then defrost in 3-5 min to +18°With, at the same time as stabilizing environment of the use of 5%glucose solution at a ratio of 1:10 with the addition of a 5%solution of chloroform or 2%salicylic acid with subsequent sublimation and sublimation is carried out in a vacuum chamber at a temperature of heated shelves up (+30° (C)-(+45° (C)when the temperature reaches of the drug (+20° (C)-(+22° (C) the temperature of the shelves is reduced to +25°and produce a final drying for 22-24 hours



 

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