Method for treating exacerbations in case of multiple sclerosis

FIELD: medicine, neurology.

SUBSTANCE: the method deals with detecting an interferon status followed by medicinal therapy. Moreover, additionally one should determine patient's body area, and at observed degree I of interferon system suppression it is necessary to prescribe "Antilympholin Kz" preparation as medicinal therapy intravenously by drops at course dosage being (0.5-0.6)g x S, where S - patient's body area, every other day, and thioctacide preparation at the dosage of 300-600 mg intravenously by drops daily at a 6-8-d-long course. In case of degrees II and III of interferon system suppression one should prescribe "Antilympholin Kz" at course dosage being 0.4-0.5 g/sq. m and 0.3 -.4 g/sq. m, as for thioctacide - it should be applied at the dosage of 600-900 mg and 900-1200 mg, correspondingly. The method enables to selectively affect the immune system efficiently due to combined prescription of an immunimodulator and a preparation that accelerates its introduction at suppressing the activity of proinflammatory cytokines that prolongs disease remission due to preventing the development of immunological disorders.

EFFECT: higher efficiency of therapy.

3 ex

 

The invention relates to medicine, namely, neurology, and is intended for the treatment of acute exacerbations of multiple sclerosis. Using the proposed method it is possible to increase the efficiency of treatment of acute exacerbations of multiple sclerosis and reduce the risk of permanent disability.

Multiple sclerosis (PC) is a chronic progressive disease of the nervous system, characterized by the occurrence of multiple lesions in the Central nervous system. Studies conducted in our country and abroad, have identified a high proportion of PC. In the pathogenesis of multiple sclerosis are essential congenital disorders of immunity, increased permeability of the blood-brain barrier, the contact of immune cells with the basic myelin protein, antibody to myelin basic protein, the destruction of the myelin sheaths of nerve fibers in the Central and peripheral nervous system.

There is a method of treatment of acute exacerbations of multiple sclerosis cyclophosphamide. The drug was administered intravenously at a dose of 3 to 5 mg/kg (200 to 400 mg) per day for 10-14 days under the control of the blood (Neretin VA et al. "Immunosuppressive therapy of multiple sclerosis", Methodical recommendations, M., MONICA, 1992, p.14-15).

The disadvantages of this method are poor tolerability of the drug the patient, high risk of complications (hematological, infectious, and changes in the gastro-intestinal tract and liver). Cyclophosphamide is a derivative of mustard gas, refers to almyroudis compounds, the mechanism of action is to join their groups to the DNA, which leads to the destruction of molecules and cytotoxic effect. The drug acts on malagelada lymphocytes and causes inhibition of humoral effect and non-specific inflammatory reactions, thus not providing a selective action on the immune cell system and contributing to the progression of unwanted immunological disorders.

As a prototype of the selected method for the treatment of multiple sclerosis immunomodulatory drugs on the basis of the analysis of interferon status of the patient: pre-determine interferon status with the subsequent appointment of drug therapy. Determine the activity of interferon in the serum of the patient and in samples of whole blood incubated in medium RPMI - 1640 in the presence of standard inductors synthesis of interferons, by titration of the samples in cell culture and subsequent incubation with vesicular stomatitis virus (Application for invention of the Russian Federation No. 98105888, IPC G 01 N 33/68, publ. 2000).

The disadvantage of this method is the low efficiency is ity of treatment due to the lack of accounting for the activity of the autoimmune process, the lack of definition of boundaries of interferon status indicators for the purpose of different types of pathogenetic therapy of multiple sclerosis and as a consequence of the deepening of the autoimmune process in multiple sclerosis.

The problem posed by the authors, is to eliminate these drawbacks by eliminating unwanted immunological disorders by selective action on the immune cell system, based on the activity of the autoimmune process, clearly defining the boundaries of interferon status indicators, allowing you to assign adequate pathogenetic therapy depending on disease activity.

For this purpose, the method of treatment of an exacerbation of multiple sclerosis, including the determination of the interferon status with subsequent drug therapy, proposed to further define the area of the patient's body, as drug therapy to prescribe the drug "Antilepton KZ" intravenous drip in the course dose (0.5 to 0.6)g×S, where S is the area of the patient's body, through the day, and thioctacid in doses of 300-600 mg/drip daily at the rate of 6-8 days when I registered the degree of inhibition of the interferon system, II and III degree of inhibition of the interferon system similar to appoint "Antilepton KS" course dose of 0.4-0.5 g/m2and 0.3-0.4 g/m2and thioctacid in e is ze 600-900 900-1200 mg and mg, respectively.

Simultaneous application of Antilymphoma KS" and thioctacid allows you to accelerate the introduction of the drug "Antilepton short circuit and increase its effect by suppressing the activity protivovospolitelnyh cytokines.

In patients with active course of multiple sclerosis, continuing after a course of immunosuppressive therapy with glucocorticoids, pre investigate interferon status and subsequent prescribed drug therapy (drug "Antilepton KZ", contributing to increasing levels of anti-inflammatory cytokine α-interferon) with regard to the definition advanced area of the patient's body. In addition, to accelerate the introduction of the drug "Antilepton short circuit and increase its effect by inhibition of apoptosis and activity of proinflammatory cytokines at the same time prescribe the drug thioctacid. When I degree of inhibition of the interferon system (α-interferon 160-640 IU/ml and γ-interferon 32-128 u/ml) prescribe the drug "Antilepton PL/drip through the day in the amount of 3-4 injections at the dose rate of 0.5-0.6 g/m2and at the same time introducing/drip thioctacid in doses of 300-600 mg daily, with grade II (α-interferon 80-160 IU/ml and γ-interferon 16-32 units/ml)- drug "Antilepton short circuit" in the course dose of 0.4-0.5 g/m2and thioctacid dose of 600-900 mg, III degree (α-interferon <8 units/ml and γ -interferon <16 u/ml) - drug "Antilepton short circuit" in exchange dose of 0.3-0.4 g/m2and thioctacid dose of 900-1200 mg

The major difference of the proposed method from the prototype is the definition of the boundaries of the interferon status indicators taking into account the activity of the pathological process that allows you to choose the optimal treatment method as that of the original reduced sharply depressed, and in a satisfactory condition interferon system, to exclude a significant decrease of these indices in patients with PC, thus preventing further progression of immunological disorders.

Example 1

Patient M., born in 1975, IB. 15295. Height 172 cm, weight 75 kg, body surface area 1.9 m2.

Diagnosed with multiple sclerosis, spinal form, the condition, the aggravation.

In the anamnesis of disease, 3 of deterioration, the present deterioration in the course of one month.

Neurological deficit at admission is on a scale FS=8 points, EDSS=3 points.

Immunological tests: the average level before treatment α-IFN - 240 u/ml, average γ-IFN - 48 u/ml (I degree of inhibition of interferon status), after treatment α-IFN - 480 u/ml, γ-IFN - 64 u/ml

Treatment: to calculate the dose of the drug "Antilepton KZ" pre-refinable by nomograms area of the skin of the patient: for a person with whom the OST 172 cm and weight 75 kg, it is S=1.9 m 2then taking into account the extent of inhibition of interferon system (patient she first degree) calculated dose rate of the drug at the rate of 0.6 g/m2(0,6×1,9=1,14 g). At the same time introduced daily/drip thioctacid when I degree of inhibition of interferon system in doses of 300-600 mg

Thus, we introduce a drug "Antilepton short circuit" in the course dose of 1.14 g/drip for 3 the introduction of a day and/drip thioctacid at a dose of 300 mg daily.

After treatment of neurological deficit scale FS=6 points, EDSS=2 points. For dynamic monitoring of the patient within 6 months of relapse of the disease was not observed.

Example 2

Patient S., born in 1963, IB. 6654. Height 168 cm, weight 70 kg, body surface area 1.8 m2. Diagnosed with multiple sclerosis, spinal form, the condition, the aggravation.

In the history of the disease - 4 exacerbation of multiple sclerosis, when the last patient received hormone therapy with a positive effect, however, after the drug was the emergence of new symptoms.

Neurological deficit at admission is on a scale FS=7 points on the EDSS scale=2 points.

Immunological examination: medium α-IFN in the IRL before treatment - 120 IU/ml after treatment - 320 u/ml, average γ-IFN in the IRL before treatment 24 IU/ml after treatment 32 IU/ml

It was conducted by the prohibited treatment: to calculate the dose of the drug "Antilepton KZ" pre-specify normograms area of the skin of the patient: for a person with height is 168 cm and weight 70 kg it is 1.8 m 2then taking into account the extent of inhibition of interferon system (patient registered with II degree) calculate the exchange rate the dose rate of 0.5 g/m2(0,5×1,8=0.9 g). At the same time introduced daily/drip thioctacid with II degree of inhibition of interferon system in the dose of 600-900 mg

Thus, we introduce a drug "Antilepton KZ" 0.9 g on the course/drip N.3 and/drip thioctacid at a dose of 900 mg daily.

After treatment of neurological deficit scale FS=5 points, on a scale EDSS=1 point, i.e. positive neurological dynamics, kept for 1 year.

Example 3

Patient J., born in 1972, IB. 21914. Height 170 cm, weight 73 kg, body surface area of 1.85 m2. Diagnosed with multiple sclerosis, spinal form, the secondary having a progressive course.

Medical history: has 2 exacerbation of the pathological process, after which the disease has been steadily progressing. The patient received prednisolone, against which there was a positive dynamics. However, if you cancel hormonal therapy neurological symptoms again increased.

Neurological deficit at admission is on a scale FS=15 points, EDSS=6 points. Immunological examination: medium α-IFN in the IRL before treatment - 80 IU/ml after treatment - 320 u/ml, average γ-IFN in the IRL before treatment 6 u/ml, after treatment - 24 IU/ml

Treatment: to calculate the dose of the drug "Antilepton KZ" pre-specify normograms area of the skin of the patient: for a person with a growth of 170 cm and a weight of 73 kg 1,85 m2then taking into account the extent of inhibition of interferon system (patient it III degree) calculated dose rate of the drug at the rate of 0.3 g/m2(0,3×1,85=0,555 g). At the same time introduced daily/drip thioctacid III-degree of inhibition of interferon system in the dose of 900-1200 mg

Thus was introduced the drug "Antilepton KZ" 0.56 g on the course/drip N.3 and/drip thioctacid at a dose of 1200 mg daily.

After treatment of neurological deficit scale FS=11 points, EDSS=5 points. Re-rate the above treatment was performed to the patient after 6 months due to symptoms of exacerbation of multiple sclerosis.

The present invention can effectively improve social rehabilitation of the patient, to improve the quality of life while reducing the economic costs associated with the disability of the patient and expenses for medications, increase the duration of remission.

Treatment of acute exacerbations of multiple sclerosis, including the determination of the interferon status with subsequent drug therapy, otlichalis the same time, what additionally is determined by the size of the patient's body, as the drug therapy prescribed drug "Antilepton KZ" intravenous drip in the course dose (0.5 to 0.6)g×S, where S is the area of the patient's body, through the day and thioctacid in doses of 300-600 mg intravenously daily for a course of 6-8 days when I registered the degree of inhibition of the interferon system, II and III degree of inhibition of the interferon system similar to appoint "Antilepton short circuit" in the course dose of 0.4-0.5 and 0.3-0.4 g/m2and thioctacid dose and 600-900 900-1200 mg, respectively.



 

Same patents:

FIELD: organic chemistry, biochemistry, medicine, pharmacy.

SUBSTANCE: invention relates to new substituted 1,3-dioxo-2,3-dihydro-1H-pyrrolo[3,4-c]quinolines of the general formula (1)

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9 cl, 19 sch, 7 tbl, 25 ex

FIELD: organic chemistry, chemical technology, medicine, pharmacy.

SUBSTANCE: invention describes derivatives of benzodiazepine of the general formula (I)

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EFFECT: improved method for preparing, valuable medicinal properties of compounds.

10 cl, 1 tbl, 173 ex

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42 cl, 106 ex

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EFFECT: pharmaceutical composition for apoptosis treatment and investigation.

6 cl, 3 dwg, 8 ex, 1 tbl

FIELD: pharmaceutical chemistry, medicine.

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EFFECT: higher efficiency of therapy.

2 cl, 6 dwg, 8 ex, 5 tbl

FIELD: organic chemistry, biochemistry, biology.

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EFFECT: improved preparing method, valuable medicinal and biochemical properties of composition.

7 cl, 1 dwg, 2 tbl, 5 ex

FIELD: pharmacology.

SUBSTANCE: one should apply glycosaminoglycanes at average molecular weight being 2400 D to prepare pharmaceutical composition for treating senile feeble-mindedness and neurological cerebral lesions induced due to sudden attack or trauma. The innovation suggested increases the number of medicinal preparations of necessary indication.

EFFECT: higher efficiency of application.

7 cl, 2 dwg, 8 tbl

FIELD: medicine, pharmacy.

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EFFECT: improved and valuable medicinal properties of agent.

6 tbl

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52
O- or -O-CR52
CR52
O-, or -O-CR52
=CR52
O-; Z represents oxygen atom (O) or sulfur atom (S); each R1 represents independently hydrogen atom (H) or (C1-C6)-alkyl; each R2 represents independently hydrogen atom (H) or (C1-C6)-alkyl, (C1-C3)-fluoroalkyl; each R4 represents independently hydrogen atom (H) or (C1-C6)-alkyl; each R5 represents hydrogen atom (H) or (C1-C6)-alkyl; n = 2, 3 or 4. Also, invention describes a method for preparing compound by cl. 1 with enantiomeric excess above 80% and relates to pharmaceutical composition for enhancing the synaptic response mediated by AMPA-receptors based on compounds by cl. 1. Pharmaceutical composition is useful for treatment of schizophrenia, schizophrenia-like behavior or depression in humans in necessary for carrying out such treatment based on compounds by cl. 1 wherein this pharmaceutical composition is useful for the memory improvement and comprising compound by cl. 1. Invention provides preparing new compounds eliciting useful biological properties.

EFFECT: valuable medicinal properties of compounds.

107 cl, 2 dwg, 2 tbl, 10 ex

FIELD: organic chemistry, medicine, pharmacy.

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EFFECT: valuable medicinal properties of compounds.

16 cl, 9 ex

FIELD: medicine, immunology.

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EFFECT: improved and effective method for sanitation.

2 cl, 2 tbl, 5 ex

FIELD: medicine, immunology, pharmacy.

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EFFECT: improved method for preparing, valuable medicinal properties of preparation.

11 cl, 1 tbl, 8 ex

FIELD: medicine, oncology.

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EFFECT: higher efficiency of inhibition.

9 dwg, 2 tbl

FIELD: medicinal biotechnology, immunology.

SUBSTANCE: invention relates to producing medicinal biological preparations. Invention relates to a method for enhancing specific activity of immunoglobulins of class G. Method involves freezing immunoglobulin G-containing fractions to temperature from -20°C to -196°C and subjected for gamma-irradiation in doses 5-50 kGr with power of absorbed dose 60-110 Gr/min. method provides elevating the specific activity of immunoglobulin G, increasing titer, avidity and reducing anti-complementary activity.

EFFECT: improved method for elevating activity of antibodies.

3 tbl, 3 dwg, 4 ex

FIELD: immunology, biotechnology, medicine.

SUBSTANCE: invention relates to antiidiotypical monoclonal antibody or fragment thereof for BSW17 antibody effecting on LgE Cε3-region bonding to high affinity LgE receptor. Amino acid sequence is as described in specification. antiidiotypical antibody is useful as pharmaceutical composition ingredient for LgE-mediated disease treatment. Invention make in possible to prevent allergic disorders and inflammations due to inhibiting interaction between LgE Cε3-region with high affinity receptor by claimed antibody.

EFFECT: new agent for allergic and inflammation disorder treatment.

7 cl, 32 dwg, 5 tbl, 10 ex

FIELD: veterinary science.

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EFFECT: higher efficiency of correction.

3 tbl

FIELD: medicine, pharmaceutics, pharmacology.

SUBSTANCE: one should apply mammalian anti-HBP-antibodies. The ways are being suggested to identify monoclonal antibody bound, at least, with one epitope upon native HBP (heparin-binding protein) and methods to detect whether a mammal produces HBR being bound with a monoclonal antibody and, also, the kits for the above-mentioned purpose. The present innovation provides the opportunity to apply the mentioned antibodies in preventing and treating disorders associated with bradykinin releasing.

EFFECT: higher efficiency of application.

25 cl, 11 dwg, 3 ex, 1 tbl

FIELD: pharmaceutics.

SUBSTANCE: the present innovation deals with cryoprotective ointment containing recombinant interferon-α2. The suggested cryoprotective ointment contains recombinant interferon-α2, glycerol, polyethylene glycol 300-6000, polyglucin, buffered 0.02%-Trilon B solution at pH of 5.5-7.0 and ointment foundation at a certain content of components per 1.0 g ointment. Additionally, cryoprotective ointment could contain glycine 3,7-bis(dimethylamino)phenothiazonium chloride, dry immunoglobulin preparation or dry immunoglobulin preparation for enteral application. Ointment foundation of cryoprotective ointment could contain water-free lanolin, Vaseline and Vaseline oil, at the following ratio of components: 2.5;3.5:1 - 6.5:0.5:1. The innovation provides maximal safety of recombinant interferon-α2 activity in cryoprotective ointment at multiple alteration of positive and negative environmental temperature and at keeping cryoprotective ointment under these conditions.

EFFECT: higher efficiency of application.

8 cl, 8 ex

FIELD: medicine and immunology, in particular treatment and prevention immunodeficiency conditions and diseases associated with bacterial or viral aggression.

SUBSTANCE: claimed method includes administration to a patient immunoglobulin drug (e.g., pharmaceutical composition containing 6-12 % of specific heterologous secreted immunoglobulin A, isolated from milk or foremilk of immunized ungulates). Administration is performed parenterally wherein single dose is at least 10 IU/kg of patient weight for treatment or at least 5 IU/kg for prophylaxis; or perorally in dose of 0.2-0.5 g and/or topically one-two times per day for 1-5 days. Method of present invention makes it possible to decrease dose of administrating immunoglobulin due to prolonged retention of its high titers in body fluids.

EFFECT: enlarged range of application and assortment of immunoglobulin drugs.

4 cl, 5 ex

FIELD: microbiology and immunology, in particular immunodiagnosis.

SUBSTANCE: atypical strain of melioidose Burkholderia pseudomallei-111-6-1 with altered phenotype defected with respect to synthesis of 8 antigen and acting as immunosuppressor is used as antigen for animal immunization. Immune serum is obtained after 2 immunization cycles of animal-producer with titer in gel immunodiffusion reaction not less than 1:128.

EFFECT: immune serum with increased specific activity.

2 tbl, 2 ex

FIELD: organic chemistry, medicine, pharmacology, biochemistry, pharmacy.

SUBSTANCE: invention proposes poly-(para-dihydroxy-para-phenylene)-thiosulfoacid sodium salt of the general formula:

wherein n = 2-6 as a substance regulating cell metabolism and eliciting anti-hypoxic properties. Also, invention proposes a pharmaceutical composition based on the indicated compound. Applying the compound provides the expressed anti-hypoxic effect. Invention can be used in cosmetology and food industry and relates to preparations regulating metabolism in separated cells and their consortium in biological tissues.

EFFECT: valuable biological and medicinal properties of compound.

4 cl, 10 tbl, 4 dwg, 6 ex

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