Lipid structure-phase conversions in aqueous solutions

FIELD: medicine, pharmacology, biology, agriculture.

SUBSTANCE: method relates to lipid structure-phase conversions in aqueous solutions, which represent important component information recording/sensing on the level of brain synaptic membranes. To perform lipid structure-phase conversions in aqueous solutions in presence of additives lipid aqueous solution and additive aqueous solution are divided by water layer being separated from said solution by membranes which are permeable only for water molecules. Lipid components of animal, plant cells and surfactants of lipid origin are used as lipids, and water-soluble substances of inorganic, organic or biological origin are used as additives.

EFFECT: method for investigation of various substance effects on lipid structure-phase conversions in aqueous solutions.

4 cl, 5 ex, 1 dwg

 

The invention relates to structural-phase transformations of lipids in aqueous solutions, which are an important element of the functioning mechanism of the recording/reading information on the level of synaptic membranes of the brain, and can be used in medicine, pharmacology, biology, and agriculture.

You know the change of structural-phase transformations of lipids in aqueous solutions (phase transition temperature, square hysteresis and others) under the action β-(4-hydroxy, 3,5-ditretbutyl-)-phenylpropionic acid, adrenocorticotropic hormone, added directly to the solution of lipids (Kazarman P.A., Arkhipova GV Biophysics, 2002, T, No. 6, s-1133).

An object of the invention is the implementation of structural-phase transformations of lipids in aqueous solutions under the action of additives, which are separated from the solutions of the lipid layer of water, concluded between permeable only to water molecules membranes.

The technical result is achieved in that in the method of implementation of the structural-phase transitions of lipids in aqueous solution under the action of additives aqueous solution of lipids and aqueous solution additives separated by a layer of water, which is separated from these solutions permeable only to water molecules membranes, and then determine the parameter of structural-phase transformations, such as PFC. In kacestvenaja use of lipid components of cell membranes of animals, plants, surface-active substances (surfactants) lipid nature. As additives used water-soluble inorganic substances, organic or biological origin.

Structural-phase transformations in aqueous solutions of lipids under the influence of the additives depend on the influence of the latter on the structure of water. The continuum concept is water as a uniform grid of H-bonds of water molecules in the entire space of the device, where there are different energy H-bond, and supplements strengthen or weaken collective intermolecular interactions of water, which affects the structural-phase transformations of lipids, even through the layer of water separating the solution of the additive from a solution of lipid permeable only to water molecules membranes.

The method is as follows.

In the vessel 1 of the device shown in the drawing, was placed a solution of lipid or mixtures thereof with other substances. In vessel 2 is placed aqueous solution of the additive. In the vessel 3, separated from the vessels 1 and 2 two diaphragms 4, put water. Use pure distilled water, or pyrophoric (without bacteria), or water. To eliminate the hydraulic effect of levels of water and fluids in the vessels should be the same. Then in the vessel 1 observe structural-phase transformations of lipids to change the critical concentration is yellowbrown (ECR). Determination of CMC technically easier and more accurate than the determination of the temperature of phase transitions and hysteresis in the curves of phase transitions, which are not applicable for solving technical problems. An aqueous solution of lipids and aqueous solution of additives in the vessels 1 and 3 can move relative to the membrane 4.

To determine the temperature structural phase transition the device must be placed in the calorimeter cell, which represents a technical challenge.

The invention is illustrated by examples.

Example 1. In the same vessels 1 and 2 volume of 70 ml put the same number, 65 ml solutions of sodium dodecyl sulfate (DDS) of the same concentration (s). In the vessel 3, which is connected through a permeable only to water molecules LM membranes Thomapor-0,5 (4) vessels 1 and 2, put water. The diameter of pores of the membranes 4 (1-5)·10-3Ám. The membrane 4 is not passed hydrated sodium ions and DDS, but allow the water molecules. The device is made of stainless steel or titanium for the absence of interfering ions. The connection of the vessels 1, 2 and 3 are made on the threads so that the membrane 4 can be changed. To exclude the effects of structured water in premembrane layer on ECR DDS membrane 4 is made of the same diameter. The distance between the membranes 4 100 mm After the establishment of the adsorption equilibrium on the boundary of the solution with the air in which estorach vessels 1 and 2 by the method of the plates for each concentration DDS measure the surface tension (σ and the inflection dependence σ=f(ln C) determine the CMC VAT at 20±0,5°C. PFC spherical micelles DDS in vessels 1 and 2 is equal to 6,0·10-3mol/l CMC does not change, as the impact of DDS solutions in the vessels 1 and 2 to each other in the same way.

Example 2. In the vessel 1 is placed DDS solutions, and in the vessel 2 - NaCl solutions of the same concentration. In the vessel 3 pour distilled water. Once equilibrium has been established which monitor the achievement of a constant value of surface tension of fluids in the vessel 1, according to σ=f(ln C) determine the CMC DDS - 3,3-10-3mol/L. It is reduced compared to example 1. This parameter determines the structure of water changes, and micelles DDS rebuilt.

Example 3. Study the influence (4H9)4NI on ECR DDS, as in example 2, which was equal to 0.8·10-3mol/l, i.e. decreased.

Example 4. Learn about the impact of β-(4-hydroxy, 3,5-ditretbutyl-)-phenylpropionic acid CMC DDS, as in example 2, which was equal to 0.2·10-3mol/l, i.e. has also changed.

Example 5. Learn about the impact of β-(4-hydroxy, 3,5-ditretbutyl-)-phenylpropionic acid on MCF depletability. It decreases from 5·10-10mol/l without the addition of 0.1·10-10mol/l with the addition of β-(4-hydroxy, 3,5-ditretbutyl-)-phenylpropionic acid. KCM lipids and lepidopter what's surfactants in examples 3-5 decreases, therefore, occur the structural-phase transformations.

Thus, additives such as NaCl, (C4H9)4NI β-(4-hydroxy, 3,5-ditretbutyl-)-phenylpropionic acid through the layer of water carry out structural-phase transformations in aqueous solutions of lipids and change the structure of water. KCM is a parameter characterizing the structural-phase transformations in solutions of lipid components of cell membranes of animals, plants, surface-active substances of lipid nature.

1. Method for making structural-phase transitions of lipids in aqueous solution in the presence of additives, characterized in that the aqueous solution of lipids and aqueous solution additives divide the water layer separated from these solutions permeable only to water molecules membranes.

2. The method according to claim 1, characterized in that the lipids used lipid components of cell membranes of plants, animals and surfactant lipid nature.

3. The method according to claim 1, characterized in that the additives used water-soluble inorganic substances, organic or biological origin.

4. The method according to claim 1, characterized in that aqueous solutions of lipids and additives are stationary or may move relative to the membrane.



 

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